SPHK1 cDNA ORF clone, Ficedula albicollis(collared flycatcher)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPHK1
Entrez Gene ID	101818075
Full Name	sphingosine kinase 1
Gene Type	protein-coding
Organism	Ficedula albicollis(collared flycatcher)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005055946.2	XP_005056003.1	sphingosine kinase 1

Pathways from BioSystems
Gene Ontology

The following SPHK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPHK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFi06603	XM_005055946.2 Latest version!	Ficedula albicollis sphingosine kinase 1 (SPHK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPHK1 ( XM_005055946.2 ) cDNA ORF clone, Ficedula albicollis(collared flycatcher) -> XP_005056003.1 Ficedula albicollis sphingosine kinase 1 (SPHK1), mRNA.

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CloneID	OFi06603
Clone ID Related Accession (Same CDS sequence)	XM_005055946.2
Accession Version	XM_005055946.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-19
Organism	Ficedula albicollis(collared flycatcher)
Product	sphingosine kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_021689.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2016 this sequence version replaced XM_005055946.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ficedula albicollis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCCG AGGCAGATAT TGCCACCACT GTCTTCGTCA CTGAGAGACC CCACCACGCC 
CATGAGAAGG TGCGAGATGA GGACCTGTCG CAGTGGGACA CCTTGGTGGT CATGTCTGGG 
GACGGGCTGC TGTTCGAGGT GGTGAACGGG CTGATGGAGC GCCCAGACTG GAAGGAGGCC 
ATGAAGAAGC CGCTGTGCAT CCTGCCAGGA GGCTCTGGGA ATGCCCTGGC TGCCTCTATC 
AACTACTATG CAGGCTACGA CCATGTCGCC AAGAAAAAGC TGCTGACGAA TTGCACCTTC 
ATCCTGTGCA AGGGGCTGTA CACGCAGATG GACCTGGTGT CACTGAGCAC GGCCTCGGGC 
AAGCACTTCT TCTCCTTCCT GGGCTTTGGC TGGGGCTTCA TCTCGGACGT GGACATCGAC 
AGCGAGAAGT ACCGCTGGCT GGGCAGCGCC CGCTTCACCC TGGGCACGCT GCAGTGCCTG 
GCCAAGCTGC GGGTGTACCA GGGCCGCCTG TCCTACCTGC CCGTGGCCGC GGAGCAGGGC 
AGCTCCCCGG AGCCCCGGGA CCCCCGCGCG CCCCTCACCA ACGGCCACAT CCCGCAGGCC 
GCGGGGACGG GCGCGCCCGG CTCGCTGCCC CCCGACTCGC TGCTGGCGCC GCTGGGCCAG 
CCCGTGCCCG CGCATTGGAC CGTGGTCCCC GAGGAGGAGT TTGTGCTGGT CTATGCCATC 
TACCAGTCCC ACCTGGGCAC CAACCTGCTG ATGGCGCCGG CGGCCCGGCT GCACGACGGC 
GTCATCCACC TCTTCTACAT GAAGGCCGGC ATCAGCCGCG TGACGCTGCT GAAGCTCTTC 
CTGGCCATGT CCAGGGGAAC GCACCTGGAC TTGAACTGTC CCCACCTGTG CTACGTCCCC 
GTGCGGGCGT TCCGCCTGGA GCCGCGGGTG GCCGCGGGCA TCATGACCGT GGATGGGGAG 
GCGCTGGCCT GCGAGCCCGT GCAGGGCCAG GTCCATGCCC GCCTCTGCCG CGTCCTCAGC 
GGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005056003.1
CDS	108..1136
Translation	MLAEADIATTVFVTERPHHAHEKVRDEDLSQWDTLVVMSGDGLLFEVVNGLMERPDWKEAMKKPLCILPGGSGNALAASINYYAGYDHVAKKKLLTNCTFILCKGLYTQMDLVSLSTASGKHFFSFLGFGWGFISDVDIDSEKYRWLGSARFTLGTLQCLAKLRVYQGRLSYLPVAAEQGSSPEPRDPRAPLTNGHIPQAAGTGAPGSLPPDSLLAPLGQPVPAHWTVVPEEEFVLVYAIYQSHLGTNLLMAPAARLHDGVIHLFYMKAGISRVTLLKLFLAMSRGTHLDLNCPHLCYVPVRAFRLEPRVAAGIMTVDGEALACEPVQGQVHARLCRVLSGS

Target ORF information:

RefSeq Version	XM_005055946.2
Organism	Ficedula albicollis(collared flycatcher)
Definition	Ficedula albicollis sphingosine kinase 1 (SPHK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005055946.2

ORF Insert Sequence:

ATGCTGGCCG AGGCAGATAT TGCCACCACT GTCTTCGTCA CTGAGAGACC CCACCACGCC 
CATGAGAAGG TGCGAGATGA GGACCTGTCG CAGTGGGACA CCTTGGTGGT CATGTCTGGG 
GACGGGCTGC TGTTCGAGGT GGTGAACGGG CTGATGGAGC GCCCAGACTG GAAGGAGGCC 
ATGAAGAAGC CGCTGTGCAT CCTGCCAGGA GGCTCTGGGA ATGCCCTGGC TGCCTCTATC 
AACTACTATG CAGGCTACGA CCATGTCGCC AAGAAAAAGC TGCTGACGAA TTGCACCTTC 
ATCCTGTGCA AGGGGCTGTA CACGCAGATG GACCTGGTGT CACTGAGCAC GGCCTCGGGC 
AAGCACTTCT TCTCCTTCCT GGGCTTTGGC TGGGGCTTCA TCTCGGACGT GGACATCGAC 
AGCGAGAAGT ACCGCTGGCT GGGCAGCGCC CGCTTCACCC TGGGCACGCT GCAGTGCCTG 
GCCAAGCTGC GGGTGTACCA GGGCCGCCTG TCCTACCTGC CCGTGGCCGC GGAGCAGGGC 
AGCTCCCCGG AGCCCCGGGA CCCCCGCGCG CCCCTCACCA ACGGCCACAT CCCGCAGGCC 
GCGGGGACGG GCGCGCCCGG CTCGCTGCCC CCCGACTCGC TGCTGGCGCC GCTGGGCCAG 
CCCGTGCCCG CGCATTGGAC CGTGGTCCCC GAGGAGGAGT TTGTGCTGGT CTATGCCATC 
TACCAGTCCC ACCTGGGCAC CAACCTGCTG ATGGCGCCGG CGGCCCGGCT GCACGACGGC 
GTCATCCACC TCTTCTACAT GAAGGCCGGC ATCAGCCGCG TGACGCTGCT GAAGCTCTTC 
CTGGCCATGT CCAGGGGAAC GCACCTGGAC TTGAACTGTC CCCACCTGTG CTACGTCCCC 
GTGCGGGCGT TCCGCCTGGA GCCGCGGGTG GCCGCGGGCA TCATGACCGT GGATGGGGAG 
GCGCTGGCCT GCGAGCCCGT GCAGGGCCAG GTCCATGCCC GCCTCTGCCG CGTCCTCAGC 
GGCTCCTGA