dbt cDNA ORF clone, Esox lucius(northern pike)

Gene
Clones
gRNAs

Summary

Gene Symbol	dbt
Entrez Gene ID	105011373
Full Name	dihydrolipoamide branched chain transacylase E2
Gene Type	protein-coding
Organism	Esox lucius(northern pike)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010871353.3	XP_010869655.1	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X2
XM_010871352.2	XP_010869654.1	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X1

Pathways from BioSystems
Gene Ontology

The following dbt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dbt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEs14876	XM_010871352.2 Latest version!	Esox lucius dihydrolipoamide branched chain transacylase E2 (dbt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OEs14877	XM_010871353.3 Latest version!	Esox lucius dihydrolipoamide branched chain transacylase E2 (dbt), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dbt ( XM_010871352.2 ) cDNA ORF clone, Esox lucius(northern pike) -> XP_010869654.1 Esox lucius dihydrolipoamide branched chain transacylase E2 (dbt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEs14876
Clone ID Related Accession (Same CDS sequence)	XM_010871352.2
Accession Version	XM_010871352.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-09
Organism	Esox lucius(northern pike)
Product	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_025975.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 30, 2017 this sequence version replaced XM_010871352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Esox lucius Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG TTACTAGAGG CTCATTCACG TTAATGAGAC GTTTGCGGTT ATCATCGCAA 
CTCTCAGTAC ATCGGTACAA GCGTTGCTGC TCCAGGGTCC AAACTGGCCG ACCAGACTAC 
ACCCTCCATC CCCACTCCGT CTTCATCTCT CAACTGTGCA GCGCTAGGTT TTTCCACACT 
AGTTATGTCA AACCTGGACC AATTCTACCA TTCAAACTCT CAGATATTGG CGAGGGTATC 
ATGGAGGTGA CAGTGAAGGA ATGGTATGTT AAGGAGGGAG ACAAAGTCTC ACAGTTTGAC 
AGCATCTGTG AGGTACAGAG TGACAAAGCG TCTGTCACCA TCACAAGCCG CTTTGACGGT 
GTCATAAAAA AGCTGTACTA TGAAGTGGAC GACACTGCCT TAGTGGGGAC ACCGCTGGTG 
GACATCGAGA CAGAGTCCGG TCCGGAGGTG GCCCATGAGG AGGATGTCGT GGAGACCCCA 
GCTATGTCAC ACGATGAGCA CACACACCAG GAAATCAAAG GCCACAAGAC CCAAGCCACC 
CCGGCCGTCC GTCGCTTAGC CATGGAGAAC AATATCAAGC TAAGTGAAGT GGTCGGAACC 
GGGAGAGATG GGAGGATCCT CAAAGAGGAC ATCCTCAACT TCCTGGCGAA ACAGACTGGG 
GCAATCTTAC CACCGACTCC ATTCCATGAG ATCCAGCCCC CTCCACCGGC AGTGTCTACT 
CTTTCCTCAG CCACCAAGCC CAAGGCGAAA ACCCCAAGTG TCCCACTGGC TGTTATTTCC 
AGGCCTGTCT TCACGGGCAA GGACAGGACT GAACCCCTGA AAGGTTTCCA CAAAGCAATG 
GTGAAGACCA TGACGGCAGC CTTGAAGATC CCCCACTTTG GTTATAAAGA TGAAGTGGAT 
TTGACAAGGC TGGTGCAGCT GCGCAACGAG CTGAAGGGCC TCTCAGAGGC TCGTGGGGTC 
AAGCTCAGCT TCATGCCCTT CTTCATCAAG GCTGCATCTC TTGGTCTTCT TCATTTCCCC 
ATCCTCAACG CCTCCGTGGA CGAGGCTGTT CAGAACATCA CCTACAAGGC CTCCCACAAC 
ATTGGCTTGG CAATGGACAC CAGCCAGGGC CTGTTGGTGC CCAATGTGAA GAATGTGCAG 
ACACTGAGTG TGTTTGAGAT TGCCATGGAG CTCAACCGCA TGCAGAGCCT GGGAGCCACG 
GGCCAGCTGG GCACGCCAGA CCTCACCGGC GGCACCTTCA CCCTCTCCAA CATTGGATCG 
ATCGGAGGCA CATATGCAAA GCCAGTAATT CTGCCTCCTG AGGTGGCGAT TGGAGCTCTG 
GGAAAGATCC AGGTGCTGCC CAGGTTTAAC TTGAGGGACG AGGTGGTGAA GGCTCATATC 
ATGAACGTCA GCTGGTCGGC GGACCACCGA ATCATCGACG GGGCCACAAT GGCACGGTTC 
TCAAACCTGT GGAGAGGCTT CCTGGAAAAC CCGGCGTCCA TGATTCTGGA CCTAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010869654.1
CDS	73..1572
Translation	MAAVTRGSFTLMRRLRLSSQLSVHRYKRCCSRVQTGRPDYTLHPHSVFISQLCSARFFHTSYVKPGPILPFKLSDIGEGIMEVTVKEWYVKEGDKVSQFDSICEVQSDKASVTITSRFDGVIKKLYYEVDDTALVGTPLVDIETESGPEVAHEEDVVETPAMSHDEHTHQEIKGHKTQATPAVRRLAMENNIKLSEVVGTGRDGRILKEDILNFLAKQTGAILPPTPFHEIQPPPPAVSTLSSATKPKAKTPSVPLAVISRPVFTGKDRTEPLKGFHKAMVKTMTAALKIPHFGYKDEVDLTRLVQLRNELKGLSEARGVKLSFMPFFIKAASLGLLHFPILNASVDEAVQNITYKASHNIGLAMDTSQGLLVPNVKNVQTLSVFEIAMELNRMQSLGATGQLGTPDLTGGTFTLSNIGSIGGTYAKPVILPPEVAIGALGKIQVLPRFNLRDEVVKAHIMNVSWSADHRIIDGATMARFSNLWRGFLENPASMILDLK

Target ORF information:

RefSeq Version	XM_010871352.2
Organism	Esox lucius(northern pike)
Definition	Esox lucius dihydrolipoamide branched chain transacylase E2 (dbt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010871352.2

ORF Insert Sequence:

ATGGCGGCCG TTACTAGAGG CTCATTCACG TTAATGAGAC GTTTGCGGTT ATCATCGCAA 
CTCTCAGTAC ATCGGTACAA GCGTTGCTGC TCCAGGGTCC AAACTGGCCG ACCAGACTAC 
ACCCTCCATC CCCACTCCGT CTTCATCTCT CAACTGTGCA GCGCTAGGTT TTTCCACACT 
AGTTATGTCA AACCTGGACC AATTCTACCA TTCAAACTCT CAGATATTGG CGAGGGTATC 
ATGGAGGTGA CAGTGAAGGA ATGGTATGTT AAGGAGGGAG ACAAAGTCTC ACAGTTTGAC 
AGCATCTGTG AGGTACAGAG TGACAAAGCG TCTGTCACCA TCACAAGCCG CTTTGACGGT 
GTCATAAAAA AGCTGTACTA TGAAGTGGAC GACACTGCCT TAGTGGGGAC ACCGCTGGTG 
GACATCGAGA CAGAGTCCGG TCCGGAGGTG GCCCATGAGG AGGATGTCGT GGAGACCCCA 
GCTATGTCAC ACGATGAGCA CACACACCAG GAAATCAAAG GCCACAAGAC CCAAGCCACC 
CCGGCCGTCC GTCGCTTAGC CATGGAGAAC AATATCAAGC TAAGTGAAGT GGTCGGAACC 
GGGAGAGATG GGAGGATCCT CAAAGAGGAC ATCCTCAACT TCCTGGCGAA ACAGACTGGG 
GCAATCTTAC CACCGACTCC ATTCCATGAG ATCCAGCCCC CTCCACCGGC AGTGTCTACT 
CTTTCCTCAG CCACCAAGCC CAAGGCGAAA ACCCCAAGTG TCCCACTGGC TGTTATTTCC 
AGGCCTGTCT TCACGGGCAA GGACAGGACT GAACCCCTGA AAGGTTTCCA CAAAGCAATG 
GTGAAGACCA TGACGGCAGC CTTGAAGATC CCCCACTTTG GTTATAAAGA TGAAGTGGAT 
TTGACAAGGC TGGTGCAGCT GCGCAACGAG CTGAAGGGCC TCTCAGAGGC TCGTGGGGTC 
AAGCTCAGCT TCATGCCCTT CTTCATCAAG GCTGCATCTC TTGGTCTTCT TCATTTCCCC 
ATCCTCAACG CCTCCGTGGA CGAGGCTGTT CAGAACATCA CCTACAAGGC CTCCCACAAC 
ATTGGCTTGG CAATGGACAC CAGCCAGGGC CTGTTGGTGC CCAATGTGAA GAATGTGCAG 
ACACTGAGTG TGTTTGAGAT TGCCATGGAG CTCAACCGCA TGCAGAGCCT GGGAGCCACG 
GGCCAGCTGG GCACGCCAGA CCTCACCGGC GGCACCTTCA CCCTCTCCAA CATTGGATCG 
ATCGGAGGCA CATATGCAAA GCCAGTAATT CTGCCTCCTG AGGTGGCGAT TGGAGCTCTG 
GGAAAGATCC AGGTGCTGCC CAGGTTTAAC TTGAGGGACG AGGTGGTGAA GGCTCATATC 
ATGAACGTCA GCTGGTCGGC GGACCACCGA ATCATCGACG GGGCCACAAT GGCACGGTTC 
TCAAACCTGT GGAGAGGCTT CCTGGAAAAC CCGGCGTCCA TGATTCTGGA CCTAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dbt ( XM_010871353.3 ) cDNA ORF clone, Esox lucius(northern pike) -> XP_010869655.1 Esox lucius dihydrolipoamide branched chain transacylase E2 (dbt), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEs14877
Clone ID Related Accession (Same CDS sequence)	XM_010871353.3
Accession Version	XM_010871353.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-09
Organism	Esox lucius(northern pike)
Product	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_025975.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 30, 2017 this sequence version replaced XM_010871353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Esox lucius Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTTAT CATCGCAACT CTCAGTACAT CGGTACAAGC GTTGCTGCTC CAGGGTCCAA 
ACTGGCCGAC CAGACTACAC CCTCCATCCC CACTCCGTCT TCATCTCTCA ACTGTGCAGC 
GCTAGGTTTT TCCACACTAG TTATGTCAAA CCTGGACCAA TTCTACCATT CAAACTCTCA 
GATATTGGCG AGGGTATCAT GGAGGTGACA GTGAAGGAAT GGTATGTTAA GGAGGGAGAC 
AAAGTCTCAC AGTTTGACAG CATCTGTGAG GTACAGAGTG ACAAAGCGTC TGTCACCATC 
ACAAGCCGCT TTGACGGTGT CATAAAAAAG CTGTACTATG AAGTGGACGA CACTGCCTTA 
GTGGGGACAC CGCTGGTGGA CATCGAGACA GAGTCCGGTC CGGAGGTGGC CCATGAGGAG 
GATGTCGTGG AGACCCCAGC TATGTCACAC GATGAGCACA CACACCAGGA AATCAAAGGC 
CACAAGACCC AAGCCACCCC GGCCGTCCGT CGCTTAGCCA TGGAGAACAA TATCAAGCTA 
AGTGAAGTGG TCGGAACCGG GAGAGATGGG AGGATCCTCA AAGAGGACAT CCTCAACTTC 
CTGGCGAAAC AGACTGGGGC AATCTTACCA CCGACTCCAT TCCATGAGAT CCAGCCCCCT 
CCACCGGCAG TGTCTACTCT TTCCTCAGCC ACCAAGCCCA AGGCGAAAAC CCCAAGTGTC 
CCACTGGCTG TTATTTCCAG GCCTGTCTTC ACGGGCAAGG ACAGGACTGA ACCCCTGAAA 
GGTTTCCACA AAGCAATGGT GAAGACCATG ACGGCAGCCT TGAAGATCCC CCACTTTGGT 
TATAAAGATG AAGTGGATTT GACAAGGCTG GTGCAGCTGC GCAACGAGCT GAAGGGCCTC 
TCAGAGGCTC GTGGGGTCAA GCTCAGCTTC ATGCCCTTCT TCATCAAGGC TGCATCTCTT 
GGTCTTCTTC ATTTCCCCAT CCTCAACGCC TCCGTGGACG AGGCTGTTCA GAACATCACC 
TACAAGGCCT CCCACAACAT TGGCTTGGCA ATGGACACCA GCCAGGGCCT GTTGGTGCCC 
AATGTGAAGA ATGTGCAGAC ACTGAGTGTG TTTGAGATTG CCATGGAGCT CAACCGCATG 
CAGAGCCTGG GAGCCACGGG CCAGCTGGGC ACGCCAGACC TCACCGGCGG CACCTTCACC 
CTCTCCAACA TTGGATCGAT CGGAGGCACA TATGCAAAGC CAGTAATTCT GCCTCCTGAG 
GTGGCGATTG GAGCTCTGGG AAAGATCCAG GTGCTGCCCA GGTTTAACTT GAGGGACGAG 
GTGGTGAAGG CTCATATCAT GAACGTCAGC TGGTCGGCGG ACCACCGAAT CATCGACGGG 
GCCACAATGG CACGGTTCTC AAACCTGTGG AGAGGCTTCC TGGAAAACCC GGCGTCCATG 
ATTCTGGACC TAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010869655.1
CDS	269..1726
Translation	MRLSSQLSVHRYKRCCSRVQTGRPDYTLHPHSVFISQLCSARFFHTSYVKPGPILPFKLSDIGEGIMEVTVKEWYVKEGDKVSQFDSICEVQSDKASVTITSRFDGVIKKLYYEVDDTALVGTPLVDIETESGPEVAHEEDVVETPAMSHDEHTHQEIKGHKTQATPAVRRLAMENNIKLSEVVGTGRDGRILKEDILNFLAKQTGAILPPTPFHEIQPPPPAVSTLSSATKPKAKTPSVPLAVISRPVFTGKDRTEPLKGFHKAMVKTMTAALKIPHFGYKDEVDLTRLVQLRNELKGLSEARGVKLSFMPFFIKAASLGLLHFPILNASVDEAVQNITYKASHNIGLAMDTSQGLLVPNVKNVQTLSVFEIAMELNRMQSLGATGQLGTPDLTGGTFTLSNIGSIGGTYAKPVILPPEVAIGALGKIQVLPRFNLRDEVVKAHIMNVSWSADHRIIDGATMARFSNLWRGFLENPASMILDLK

Target ORF information:

RefSeq Version	XM_010871353.3
Organism	Esox lucius(northern pike)
Definition	Esox lucius dihydrolipoamide branched chain transacylase E2 (dbt), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010871353.3

ORF Insert Sequence:

ATGCGGTTAT CATCGCAACT CTCAGTACAT CGGTACAAGC GTTGCTGCTC CAGGGTCCAA 
ACTGGCCGAC CAGACTACAC CCTCCATCCC CACTCCGTCT TCATCTCTCA ACTGTGCAGC 
GCTAGGTTTT TCCACACTAG TTATGTCAAA CCTGGACCAA TTCTACCATT CAAACTCTCA 
GATATTGGCG AGGGTATCAT GGAGGTGACA GTGAAGGAAT GGTATGTTAA GGAGGGAGAC 
AAAGTCTCAC AGTTTGACAG CATCTGTGAG GTACAGAGTG ACAAAGCGTC TGTCACCATC 
ACAAGCCGCT TTGACGGTGT CATAAAAAAG CTGTACTATG AAGTGGACGA CACTGCCTTA 
GTGGGGACAC CGCTGGTGGA CATCGAGACA GAGTCCGGTC CGGAGGTGGC CCATGAGGAG 
GATGTCGTGG AGACCCCAGC TATGTCACAC GATGAGCACA CACACCAGGA AATCAAAGGC 
CACAAGACCC AAGCCACCCC GGCCGTCCGT CGCTTAGCCA TGGAGAACAA TATCAAGCTA 
AGTGAAGTGG TCGGAACCGG GAGAGATGGG AGGATCCTCA AAGAGGACAT CCTCAACTTC 
CTGGCGAAAC AGACTGGGGC AATCTTACCA CCGACTCCAT TCCATGAGAT CCAGCCCCCT 
CCACCGGCAG TGTCTACTCT TTCCTCAGCC ACCAAGCCCA AGGCGAAAAC CCCAAGTGTC 
CCACTGGCTG TTATTTCCAG GCCTGTCTTC ACGGGCAAGG ACAGGACTGA ACCCCTGAAA 
GGTTTCCACA AAGCAATGGT GAAGACCATG ACGGCAGCCT TGAAGATCCC CCACTTTGGT 
TATAAAGATG AAGTGGATTT GACAAGGCTG GTGCAGCTGC GCAACGAGCT GAAGGGCCTC 
TCAGAGGCTC GTGGGGTCAA GCTCAGCTTC ATGCCCTTCT TCATCAAGGC TGCATCTCTT 
GGTCTTCTTC ATTTCCCCAT CCTCAACGCC TCCGTGGACG AGGCTGTTCA GAACATCACC 
TACAAGGCCT CCCACAACAT TGGCTTGGCA ATGGACACCA GCCAGGGCCT GTTGGTGCCC 
AATGTGAAGA ATGTGCAGAC ACTGAGTGTG TTTGAGATTG CCATGGAGCT CAACCGCATG 
CAGAGCCTGG GAGCCACGGG CCAGCTGGGC ACGCCAGACC TCACCGGCGG CACCTTCACC 
CTCTCCAACA TTGGATCGAT CGGAGGCACA TATGCAAAGC CAGTAATTCT GCCTCCTGAG 
GTGGCGATTG GAGCTCTGGG AAAGATCCAG GTGCTGCCCA GGTTTAACTT GAGGGACGAG 
GTGGTGAAGG CTCATATCAT GAACGTCAGC TGGTCGGCGG ACCACCGAAT CATCGACGGG 
GCCACAATGG CACGGTTCTC AAACCTGTGG AGAGGCTTCC TGGAAAACCC GGCGTCCATG 
ATTCTGGACC TAAAATAA