bmp2k cDNA ORF clone,

Gene
Clones
gRNAs

The following bmp2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmp2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORe49016	XM_028805601.1 Latest version!	Erpetoichthys calabaricus BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORe49017	XM_028805602.1 Latest version!	Erpetoichthys calabaricus BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmp2k ( XM_028805601.1 ) cDNA ORF clone, -> XP_028661434.1 Erpetoichthys calabaricus BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORe49016
Clone ID Related Accession (Same CDS sequence)	XM_028805601.1
Accession Version	XM_028805601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-20
Organism	Erpetoichthys calabaricus(reedfish)
Product	BMP-2-inducible protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041400.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Erpetoichthys calabaricus Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TTTCTCGTAT GCCAAAGTCG GAGAGCGGTG GCCTGGGGGG AGGATGCGGT 
TCCGCTTCGG GCTCCGGAGT CACCAGCTAC ATCGGGAGGG TGTTTGCTGT GGGCCGGTAC 
CAGGTCACTG TGGAGGAACT CATTGCCGAA GGTGGATTTT CTATTGTGTT CCTTGCTCGG 
ACCCATAGTG GAGTACGCTG TGCTTTAAAA CGAATGTATG TCAACAGTAT ACCTGATGTT 
AATATCTGCA AGCGGGAGAT AACAATTATG AAAGAGCTTT CTGGTCACAA GAATATAGTG 
GGATATTTAG ACTCTGCCAT TACTACAGTT GGCGACAGTG TCTGGGAGGT GCTTATACTC 
ATGGAGTTTT GTAAAGCAGG TCAAGTTGTG AAGCAAATGA ATCAAAGGCT GCAAACTGGG 
TTCACTGAGG CTGAAATCTT AAAAATATTC TGTGACATCT GTGAAGCTGT TGCGAGGTTA 
CATCAGTGCA AAACTCCAAT CATTCACAGG GATCTCAAGG TTGAAAACAT TTTATTAAAT 
GATCATGGCC ACTATGTACT TTGTGACTTT GGTAGCGCGA CCCACAAAGT TCTTAATCCA 
CAGAAGGATG GAGTTACCAT TGTAGAAGAT GAAATAAAAA AATACACAAC ACTGTCATAC 
AGGGCTCCAG AAATGATAAA TCTTTATGGA GGAAAGTCAA TTACTACCAA GGCTGATATA 
TGGGCACTTG GCTGCTTGTT GTACAAGCTT TGTTTTTTTA CCCTCCCATT TGGGGAGAGT 
CAAGTTGCAA TCTGTGATGG AACATTTACT ATCCCAGATA ATTCCAAATA TTCTTCTAAA 
CTTCACTGTT TAATTCGATA TATGCTTGAA CCAGATGTAG AAAGAAGGCC AGATGTCTAC 
CAAGTTTCAT ATTTTACTTT TAAGATGGCT GGAAGAGAGT GTCCTGTGCC AAACATTTCT 
AACTCTGCTA TCCCATCATT TCTTCCGGAT CCCATGACTG CCAGTGAGGC AGCTGCTAAA 
AAAAGCACGA CGAAGGCCAG AATAACTGAA TCAGTAGGAC CAACAGAAAC TTCAATTGCA 
CCAAGACAGA GACCAAAGGC TGCTTCCAAT ACGTCAAACA CAAATGTGTT GCCAATTCAA 
AACTCTGTAA CTCCAGTGAA AATGTCTGTT TCTGCTGGAA ATATTAGCAA TGGACAACAA 
GCTTTTCAGG GGCCTGGAAC TGGGCATTGC ACACCTCAAT CTCAGGGTAT CAGTCAACAG 
CATAGAGTGC TGCAACAACT TCAACAAGGA GACTTGCGTC TTCAACAGCT TCAACAGTTG 
CATCATCATC ATCAGCAGCA GCAGCAAATG CAGCAAGCAT ATGTACAACA GCAGCATCCA 
CATGTTGCCC AACAGCAGTA TATACTTCAG CACCAGATGA TGATGTTACC GGCATACCAA 
CAACAAGCTC ATTATGCTGC AATGATGCAA CAATATCAGC ATGCAATTAT TCAGCAACAA 
CAGCAGCAAC AGCAGCAACA GTTTATCACC TCACCTCAAG AATTTTCAAA GTCTTTAGCT 
TTTTCGCCAG TTGTGTCAAG CCAACCTCAC ACAGCTGCAG ATGGATCTTT TAATAGCACA 
AGGACTGGCT TAATAGACAC CGATATCATT GGTGTCCCTT TGCAGAGTGT CAGTAATCCT 
CCAGATATGT CGGGGTGGAA TCCATTTGGA GAAGACAACT TCTCCAAGCT AACAGAAGAT 
GAACTTCTGG ATAGAGAATT TGACCTTCTT AGAGCAAATA AACCTGAAGA AAGAACCTGC 
CTAGACACTA AGCCGGAATC TCATCATTCA TCACACAACC CAGTTCAGGA GGACCTTTTT 
GGCTCTGTTC CCTTTGTTTC CACTCCAGGC TCCAGCTTAG AGCATCCAGA AGAGGCCACA 
GCAGAGTCAG GGGTTGTTGC AGAGAGCCAT GGAAAAGCAG AAAAGCAAGG CCAGCTGTCC 
AAAGAACATA AGGGAGGCAA AAAAAGTGGT CCTTCTGGGC AACAAACCCG AAGAGGAGGA 
GAGGACTCGG ACAGTGACTT TGAGTCAGAT CCTCCTTCTC CAAAAAGCAG TGAGGAAGAG 
GAGCAGGAAG ACGAAGAAGC CTTAAACAGT GAGCACGGGG AGTTTAACGA CAGCAATGAC 
ACTGAGCCAG AAAATTTTGG GCAGAGACCC CTTCTGATGG ATTCTGAGGA AGAGGTTGAG 
GATGAAGAGG AGGACAAGCG GAGCTCAGAC TCTGATATTG ACCTTAGGAA GAGAAAGCAG 
CCAGGAGTCG GCCTGGGAAC TGATCACGTG GTTGGTGGAA CTGGGAATCC TGCCAACAAC 
CAAATAATAA TGCCACAAGA CCCTGGTATG GAGGCAGGAG CCGCCTTAAA GTCAGAAGTG 
GATGTGTTTG GTGCTGTGCC TTTCCTTAGA ACTGAACAGA CCAAGACCTC TGCAGAGAAC 
ATGGACATAT TTTCTCAAGC ACCATTTCAG TCACCCACCA CTGAGCAAAA TATGGATGAA 
TTTGATGTAT TCACCAAAGC GCCATTTAAT AGAAGTTTAT CCAAACCTGG GAAACCAGGA 
GAAGCAGCAC TCTTCCAGTC CCCGCCAGTT TCTCCAGACA GCATAGATAT TTTTGGCTCA 
ATTCCTTTCC AGCCACCCCA ACAATTTCCT GATTCTGGCA ATAAAGAAGA CATTTTTGGG 
CAGGTGCCAT TTGAAGACTG TTCAACGCCT CAGCAGCAAA AAACAAAGCA GAGGAGTCTC 
CAAAAACTTT CCTCCAGACA AAGACGTACA AAACAAGAAA ATACAAGTGG AAACGGGAAG 
CGGCACCATG GCACTCCCAC CAGTGGCAGA AAAACCACAA AAACGAACTA TCGTACACCG 
GAGCGCGTAC GCAGACATAA GAAAGTGGGA AGACGAGATT CTCAGAGCAG CAATGAGTTT 
TTGAACACGT CAGACTCAAA AGAGAATATC AGTTTGGCGC CACCTGATTC GAAAGACAAA 
GGGCCATCGT TGCAGCCTGA AGAAACAATA CTGGACCTGT TTGGTGCAAA GCCATTCCAT 
CCACCAGAGT CTGGCAGACT CCCACAGCAC CAAGGTTTAG GAGACAACAG AGGAGATCTC 
AACTTGAGTG GTGGGAGGCC TAGACAAAGT TCACTCCATG GTTCGTTTCA TGGTGGAGAT 
GGGTTAAAAA CAGATGATTT TGGCGCCGTG CCCTTCACAG AACTTGTAGT ACAGAGTGCC 
CCCCAGCAGG CAGCGCAGGT TGAGCTTGAT CCTTTTGGAG CAGCTCCTTT TCCCTCTAAA 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028661434.1
CDS	736..4041
Translation	MKKFSRMPKSESGGLGGGCGSASGSGVTSYIGRVFAVGRYQVTVEELIAEGGFSIVFLARTHSGVRCALKRMYVNSIPDVNICKREITIMKELSGHKNIVGYLDSAITTVGDSVWEVLILMEFCKAGQVVKQMNQRLQTGFTEAEILKIFCDICEAVARLHQCKTPIIHRDLKVENILLNDHGHYVLCDFGSATHKVLNPQKDGVTIVEDEIKKYTTLSYRAPEMINLYGGKSITTKADIWALGCLLYKLCFFTLPFGESQVAICDGTFTIPDNSKYSSKLHCLIRYMLEPDVERRPDVYQVSYFTFKMAGRECPVPNISNSAIPSFLPDPMTASEAAAKKSTTKARITESVGPTETSIAPRQRPKAASNTSNTNVLPIQNSVTPVKMSVSAGNISNGQQAFQGPGTGHCTPQSQGISQQHRVLQQLQQGDLRLQQLQQLHHHHQQQQQMQQAYVQQQHPHVAQQQYILQHQMMMLPAYQQQAHYAAMMQQYQHAIIQQQQQQQQQQFITSPQEFSKSLAFSPVVSSQPHTAADGSFNSTRTGLIDTDIIGVPLQSVSNPPDMSGWNPFGEDNFSKLTEDELLDREFDLLRANKPEERTCLDTKPESHHSSHNPVQEDLFGSVPFVSTPGSSLEHPEEATAESGVVAESHGKAEKQGQLSKEHKGGKKSGPSGQQTRRGGEDSDSDFESDPPSPKSSEEEEQEDEEALNSEHGEFNDSNDTEPENFGQRPLLMDSEEEVEDEEEDKRSSDSDIDLRKRKQPGVGLGTDHVVGGTGNPANNQIIMPQDPGMEAGAALKSEVDVFGAVPFLRTEQTKTSAENMDIFSQAPFQSPTTEQNMDEFDVFTKAPFNRSLSKPGKPGEAALFQSPPVSPDSIDIFGSIPFQPPQQFPDSGNKEDIFGQVPFEDCSTPQQQKTKQRSLQKLSSRQRRTKQENTSGNGKRHHGTPTSGRKTTKTNYRTPERVRRHKKVGRRDSQSSNEFLNTSDSKENISLAPPDSKDKGPSLQPEETILDLFGAKPFHPPESGRLPQHQGLGDNRGDLNLSGGRPRQSSLHGSFHGGDGLKTDDFGAVPFTELVVQSAPQQAAQVELDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_028805601.1
Organism	Erpetoichthys calabaricus(reedfish)
Definition	Erpetoichthys calabaricus BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028805601.1

ORF Insert Sequence:

ATGAAGAAAT TTTCTCGTAT GCCAAAGTCG GAGAGCGGTG GCCTGGGGGG AGGATGCGGT 
TCCGCTTCGG GCTCCGGAGT CACCAGCTAC ATCGGGAGGG TGTTTGCTGT GGGCCGGTAC 
CAGGTCACTG TGGAGGAACT CATTGCCGAA GGTGGATTTT CTATTGTGTT CCTTGCTCGG 
ACCCATAGTG GAGTACGCTG TGCTTTAAAA CGAATGTATG TCAACAGTAT ACCTGATGTT 
AATATCTGCA AGCGGGAGAT AACAATTATG AAAGAGCTTT CTGGTCACAA GAATATAGTG 
GGATATTTAG ACTCTGCCAT TACTACAGTT GGCGACAGTG TCTGGGAGGT GCTTATACTC 
ATGGAGTTTT GTAAAGCAGG TCAAGTTGTG AAGCAAATGA ATCAAAGGCT GCAAACTGGG 
TTCACTGAGG CTGAAATCTT AAAAATATTC TGTGACATCT GTGAAGCTGT TGCGAGGTTA 
CATCAGTGCA AAACTCCAAT CATTCACAGG GATCTCAAGG TTGAAAACAT TTTATTAAAT 
GATCATGGCC ACTATGTACT TTGTGACTTT GGTAGCGCGA CCCACAAAGT TCTTAATCCA 
CAGAAGGATG GAGTTACCAT TGTAGAAGAT GAAATAAAAA AATACACAAC ACTGTCATAC 
AGGGCTCCAG AAATGATAAA TCTTTATGGA GGAAAGTCAA TTACTACCAA GGCTGATATA 
TGGGCACTTG GCTGCTTGTT GTACAAGCTT TGTTTTTTTA CCCTCCCATT TGGGGAGAGT 
CAAGTTGCAA TCTGTGATGG AACATTTACT ATCCCAGATA ATTCCAAATA TTCTTCTAAA 
CTTCACTGTT TAATTCGATA TATGCTTGAA CCAGATGTAG AAAGAAGGCC AGATGTCTAC 
CAAGTTTCAT ATTTTACTTT TAAGATGGCT GGAAGAGAGT GTCCTGTGCC AAACATTTCT 
AACTCTGCTA TCCCATCATT TCTTCCGGAT CCCATGACTG CCAGTGAGGC AGCTGCTAAA 
AAAAGCACGA CGAAGGCCAG AATAACTGAA TCAGTAGGAC CAACAGAAAC TTCAATTGCA 
CCAAGACAGA GACCAAAGGC TGCTTCCAAT ACGTCAAACA CAAATGTGTT GCCAATTCAA 
AACTCTGTAA CTCCAGTGAA AATGTCTGTT TCTGCTGGAA ATATTAGCAA TGGACAACAA 
GCTTTTCAGG GGCCTGGAAC TGGGCATTGC ACACCTCAAT CTCAGGGTAT CAGTCAACAG 
CATAGAGTGC TGCAACAACT TCAACAAGGA GACTTGCGTC TTCAACAGCT TCAACAGTTG 
CATCATCATC ATCAGCAGCA GCAGCAAATG CAGCAAGCAT ATGTACAACA GCAGCATCCA 
CATGTTGCCC AACAGCAGTA TATACTTCAG CACCAGATGA TGATGTTACC GGCATACCAA 
CAACAAGCTC ATTATGCTGC AATGATGCAA CAATATCAGC ATGCAATTAT TCAGCAACAA 
CAGCAGCAAC AGCAGCAACA GTTTATCACC TCACCTCAAG AATTTTCAAA GTCTTTAGCT 
TTTTCGCCAG TTGTGTCAAG CCAACCTCAC ACAGCTGCAG ATGGATCTTT TAATAGCACA 
AGGACTGGCT TAATAGACAC CGATATCATT GGTGTCCCTT TGCAGAGTGT CAGTAATCCT 
CCAGATATGT CGGGGTGGAA TCCATTTGGA GAAGACAACT TCTCCAAGCT AACAGAAGAT 
GAACTTCTGG ATAGAGAATT TGACCTTCTT AGAGCAAATA AACCTGAAGA AAGAACCTGC 
CTAGACACTA AGCCGGAATC TCATCATTCA TCACACAACC CAGTTCAGGA GGACCTTTTT 
GGCTCTGTTC CCTTTGTTTC CACTCCAGGC TCCAGCTTAG AGCATCCAGA AGAGGCCACA 
GCAGAGTCAG GGGTTGTTGC AGAGAGCCAT GGAAAAGCAG AAAAGCAAGG CCAGCTGTCC 
AAAGAACATA AGGGAGGCAA AAAAAGTGGT CCTTCTGGGC AACAAACCCG AAGAGGAGGA 
GAGGACTCGG ACAGTGACTT TGAGTCAGAT CCTCCTTCTC CAAAAAGCAG TGAGGAAGAG 
GAGCAGGAAG ACGAAGAAGC CTTAAACAGT GAGCACGGGG AGTTTAACGA CAGCAATGAC 
ACTGAGCCAG AAAATTTTGG GCAGAGACCC CTTCTGATGG ATTCTGAGGA AGAGGTTGAG 
GATGAAGAGG AGGACAAGCG GAGCTCAGAC TCTGATATTG ACCTTAGGAA GAGAAAGCAG 
CCAGGAGTCG GCCTGGGAAC TGATCACGTG GTTGGTGGAA CTGGGAATCC TGCCAACAAC 
CAAATAATAA TGCCACAAGA CCCTGGTATG GAGGCAGGAG CCGCCTTAAA GTCAGAAGTG 
GATGTGTTTG GTGCTGTGCC TTTCCTTAGA ACTGAACAGA CCAAGACCTC TGCAGAGAAC 
ATGGACATAT TTTCTCAAGC ACCATTTCAG TCACCCACCA CTGAGCAAAA TATGGATGAA 
TTTGATGTAT TCACCAAAGC GCCATTTAAT AGAAGTTTAT CCAAACCTGG GAAACCAGGA 
GAAGCAGCAC TCTTCCAGTC CCCGCCAGTT TCTCCAGACA GCATAGATAT TTTTGGCTCA 
ATTCCTTTCC AGCCACCCCA ACAATTTCCT GATTCTGGCA ATAAAGAAGA CATTTTTGGG 
CAGGTGCCAT TTGAAGACTG TTCAACGCCT CAGCAGCAAA AAACAAAGCA GAGGAGTCTC 
CAAAAACTTT CCTCCAGACA AAGACGTACA AAACAAGAAA ATACAAGTGG AAACGGGAAG 
CGGCACCATG GCACTCCCAC CAGTGGCAGA AAAACCACAA AAACGAACTA TCGTACACCG 
GAGCGCGTAC GCAGACATAA GAAAGTGGGA AGACGAGATT CTCAGAGCAG CAATGAGTTT 
TTGAACACGT CAGACTCAAA AGAGAATATC AGTTTGGCGC CACCTGATTC GAAAGACAAA 
GGGCCATCGT TGCAGCCTGA AGAAACAATA CTGGACCTGT TTGGTGCAAA GCCATTCCAT 
CCACCAGAGT CTGGCAGACT CCCACAGCAC CAAGGTTTAG GAGACAACAG AGGAGATCTC 
AACTTGAGTG GTGGGAGGCC TAGACAAAGT TCACTCCATG GTTCGTTTCA TGGTGGAGAT 
GGGTTAAAAA CAGATGATTT TGGCGCCGTG CCCTTCACAG AACTTGTAGT ACAGAGTGCC 
CCCCAGCAGG CAGCGCAGGT TGAGCTTGAT CCTTTTGGAG CAGCTCCTTT TCCCTCTAAA 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bmp2k ( XM_028805602.1 ) cDNA ORF clone, -> XP_028661435.1 Erpetoichthys calabaricus BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORe49017
Clone ID Related Accession (Same CDS sequence)	XM_028805602.1
Accession Version	XM_028805602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-20
Organism	Erpetoichthys calabaricus(reedfish)
Product	BMP-2-inducible protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041400.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Erpetoichthys calabaricus Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TTTCTCGTAT GCCAAAGTCG GAGAGCGGTG GCCTGGGGGG AGGATGCGGT 
TCCGCTTCGG GCTCCGGAGT CACCAGCTAC ATCGGGAGGG TGTTTGCTGT GGGCCGGTAC 
CAGGTCACTG TGGAGGAACT CATTGCCGAA GGTGGATTTT CTATTGTGTT CCTTGCTCGG 
ACCCATAGTG GAGTACGCTG TGCTTTAAAA CGAATGTATG TCAACAGTAT ACCTGATGTT 
AATATCTGCA AGCGGGAGAT AACAATTATG AAAGAGCTTT CTGGTCACAA GAATATAGTG 
GGATATTTAG ACTCTGCCAT TACTACAGTT GGCGACAGTG TCTGGGAGGT GCTTATACTC 
ATGGAGTTTT GTAAAGCAGG TCAAGTTGTG AAGCAAATGA ATCAAAGGCT GCAAACTGGG 
TTCACTGAGG CTGAAATCTT AAAAATATTC TGTGACATCT GTGAAGCTGT TGCGAGGTTA 
CATCAGTGCA AAACTCCAAT CATTCACAGG GATCTCAAGG TTGAAAACAT TTTATTAAAT 
GATCATGGCC ACTATGTACT TTGTGACTTT GGTAGCGCGA CCCACAAAGT TCTTAATCCA 
CAGAAGGATG GAGTTACCAT TGTAGAAGAT GAAATAAAAA AATACACAAC ACTGTCATAC 
AGGGCTCCAG AAATGATAAA TCTTTATGGA GGAAAGTCAA TTACTACCAA GGCTGATATA 
TGGGCACTTG GCTGCTTGTT GTACAAGCTT TGTTTTTTTA CCCTCCCATT TGGGGAGAGT 
CAAGTTGCAA TCTGTGATGG AACATTTACT ATCCCAGATA ATTCCAAATA TTCTTCTAAA 
CTTCACTGTT TAATTCGATA TATGCTTGAA CCAGATGTAG AAAGAAGGCC AGATGTCTAC 
CAAGTTTCAT ATTTTACTTT TAAGATGGCT GGAAGAGAGT GTCCTGTGCC AAACATTTCT 
AACTCTGCTA TCCCATCATT TCTTCCGGAT CCCATGACTG CCAGTGAGGC AGCTGCTAAA 
AAAAGCACGA CGAAGGCCAG AATAACTGAA TCAGTAGGAC CAACAGAAAC TTCAATTGCA 
CCAAGACAGA GACCAAAGGC TGCTTCCAAT ACGTCAAACA CAAATGTGTT GCCAATTCAA 
AACTCTGTAA CTCCAGTGAA AATGTCTGTT TCTGCTGGAA ATATTAGCAA TGGACAACAA 
GCTTTTCAGG GGCCTGGAAC TGGGCATTGC ACACCTCAAT CTCAGGGTAT CAGTCAACAG 
CATAGAGTGC TGCAACAACT TCAACAAGGA GACTTGCGTC TTCAACAGCT TCAACAGTTG 
CATCATCATC ATCAGCAGCA GCAGCAAATG CAGCAAGCAT ATGTACAACA GCATCCACAT 
GTTGCCCAAC AGCAGTATAT ACTTCAGCAC CAGATGATGA TGTTACCGGC ATACCAACAA 
CAAGCTCATT ATGCTGCAAT GATGCAACAA TATCAGCATG CAATTATTCA GCAACAACAG 
CAGCAACAGC AGCAACAGTT TATCACCTCA CCTCAAGAAT TTTCAAAGTC TTTAGCTTTT 
TCGCCAGTTG TGTCAAGCCA ACCTCACACA GCTGCAGATG GATCTTTTAA TAGCACAAGG 
ACTGGCTTAA TAGACACCGA TATCATTGGT GTCCCTTTGC AGAGTGTCAG TAATCCTCCA 
GATATGTCGG GGTGGAATCC ATTTGGAGAA GACAACTTCT CCAAGCTAAC AGAAGATGAA 
CTTCTGGATA GAGAATTTGA CCTTCTTAGA GCAAATAAAC CTGAAGAAAG AACCTGCCTA 
GACACTAAGC CGGAATCTCA TCATTCATCA CACAACCCAG TTCAGGAGGA CCTTTTTGGC 
TCTGTTCCCT TTGTTTCCAC TCCAGGCTCC AGCTTAGAGC ATCCAGAAGA GGCCACAGCA 
GAGTCAGGGG TTGTTGCAGA GAGCCATGGA AAAGCAGAAA AGCAAGGCCA GCTGTCCAAA 
GAACATAAGG GAGGCAAAAA AAGTGGTCCT TCTGGGCAAC AAACCCGAAG AGGAGGAGAG 
GACTCGGACA GTGACTTTGA GTCAGATCCT CCTTCTCCAA AAAGCAGTGA GGAAGAGGAG 
CAGGAAGACG AAGAAGCCTT AAACAGTGAG CACGGGGAGT TTAACGACAG CAATGACACT 
GAGCCAGAAA ATTTTGGGCA GAGACCCCTT CTGATGGATT CTGAGGAAGA GGTTGAGGAT 
GAAGAGGAGG ACAAGCGGAG CTCAGACTCT GATATTGACC TTAGGAAGAG AAAGCAGCCA 
GGAGTCGGCC TGGGAACTGA TCACGTGGTT GGTGGAACTG GGAATCCTGC CAACAACCAA 
ATAATAATGC CACAAGACCC TGGTATGGAG GCAGGAGCCG CCTTAAAGTC AGAAGTGGAT 
GTGTTTGGTG CTGTGCCTTT CCTTAGAACT GAACAGACCA AGACCTCTGC AGAGAACATG 
GACATATTTT CTCAAGCACC ATTTCAGTCA CCCACCACTG AGCAAAATAT GGATGAATTT 
GATGTATTCA CCAAAGCGCC ATTTAATAGA AGTTTATCCA AACCTGGGAA ACCAGGAGAA 
GCAGCACTCT TCCAGTCCCC GCCAGTTTCT CCAGACAGCA TAGATATTTT TGGCTCAATT 
CCTTTCCAGC CACCCCAACA ATTTCCTGAT TCTGGCAATA AAGAAGACAT TTTTGGGCAG 
GTGCCATTTG AAGACTGTTC AACGCCTCAG CAGCAAAAAA CAAAGCAGAG GAGTCTCCAA 
AAACTTTCCT CCAGACAAAG ACGTACAAAA CAAGAAAATA CAAGTGGAAA CGGGAAGCGG 
CACCATGGCA CTCCCACCAG TGGCAGAAAA ACCACAAAAA CGAACTATCG TACACCGGAG 
CGCGTACGCA GACATAAGAA AGTGGGAAGA CGAGATTCTC AGAGCAGCAA TGAGTTTTTG 
AACACGTCAG ACTCAAAAGA GAATATCAGT TTGGCGCCAC CTGATTCGAA AGACAAAGGG 
CCATCGTTGC AGCCTGAAGA AACAATACTG GACCTGTTTG GTGCAAAGCC ATTCCATCCA 
CCAGAGTCTG GCAGACTCCC ACAGCACCAA GGTTTAGGAG ACAACAGAGG AGATCTCAAC 
TTGAGTGGTG GGAGGCCTAG ACAAAGTTCA CTCCATGGTT CGTTTCATGG TGGAGATGGG 
TTAAAAACAG ATGATTTTGG CGCCGTGCCC TTCACAGAAC TTGTAGTACA GAGTGCCCCC 
CAGCAGGCAG CGCAGGTTGA GCTTGATCCT TTTGGAGCAG CTCCTTTTCC CTCTAAACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028661435.1
CDS	736..4038
Translation	MKKFSRMPKSESGGLGGGCGSASGSGVTSYIGRVFAVGRYQVTVEELIAEGGFSIVFLARTHSGVRCALKRMYVNSIPDVNICKREITIMKELSGHKNIVGYLDSAITTVGDSVWEVLILMEFCKAGQVVKQMNQRLQTGFTEAEILKIFCDICEAVARLHQCKTPIIHRDLKVENILLNDHGHYVLCDFGSATHKVLNPQKDGVTIVEDEIKKYTTLSYRAPEMINLYGGKSITTKADIWALGCLLYKLCFFTLPFGESQVAICDGTFTIPDNSKYSSKLHCLIRYMLEPDVERRPDVYQVSYFTFKMAGRECPVPNISNSAIPSFLPDPMTASEAAAKKSTTKARITESVGPTETSIAPRQRPKAASNTSNTNVLPIQNSVTPVKMSVSAGNISNGQQAFQGPGTGHCTPQSQGISQQHRVLQQLQQGDLRLQQLQQLHHHHQQQQQMQQAYVQQHPHVAQQQYILQHQMMMLPAYQQQAHYAAMMQQYQHAIIQQQQQQQQQQFITSPQEFSKSLAFSPVVSSQPHTAADGSFNSTRTGLIDTDIIGVPLQSVSNPPDMSGWNPFGEDNFSKLTEDELLDREFDLLRANKPEERTCLDTKPESHHSSHNPVQEDLFGSVPFVSTPGSSLEHPEEATAESGVVAESHGKAEKQGQLSKEHKGGKKSGPSGQQTRRGGEDSDSDFESDPPSPKSSEEEEQEDEEALNSEHGEFNDSNDTEPENFGQRPLLMDSEEEVEDEEEDKRSSDSDIDLRKRKQPGVGLGTDHVVGGTGNPANNQIIMPQDPGMEAGAALKSEVDVFGAVPFLRTEQTKTSAENMDIFSQAPFQSPTTEQNMDEFDVFTKAPFNRSLSKPGKPGEAALFQSPPVSPDSIDIFGSIPFQPPQQFPDSGNKEDIFGQVPFEDCSTPQQQKTKQRSLQKLSSRQRRTKQENTSGNGKRHHGTPTSGRKTTKTNYRTPERVRRHKKVGRRDSQSSNEFLNTSDSKENISLAPPDSKDKGPSLQPEETILDLFGAKPFHPPESGRLPQHQGLGDNRGDLNLSGGRPRQSSLHGSFHGGDGLKTDDFGAVPFTELVVQSAPQQAAQVELDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_028805602.1
Organism	Erpetoichthys calabaricus(reedfish)
Definition	Erpetoichthys calabaricus BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028805602.1

ORF Insert Sequence:

ATGAAGAAAT TTTCTCGTAT GCCAAAGTCG GAGAGCGGTG GCCTGGGGGG AGGATGCGGT 
TCCGCTTCGG GCTCCGGAGT CACCAGCTAC ATCGGGAGGG TGTTTGCTGT GGGCCGGTAC 
CAGGTCACTG TGGAGGAACT CATTGCCGAA GGTGGATTTT CTATTGTGTT CCTTGCTCGG 
ACCCATAGTG GAGTACGCTG TGCTTTAAAA CGAATGTATG TCAACAGTAT ACCTGATGTT 
AATATCTGCA AGCGGGAGAT AACAATTATG AAAGAGCTTT CTGGTCACAA GAATATAGTG 
GGATATTTAG ACTCTGCCAT TACTACAGTT GGCGACAGTG TCTGGGAGGT GCTTATACTC 
ATGGAGTTTT GTAAAGCAGG TCAAGTTGTG AAGCAAATGA ATCAAAGGCT GCAAACTGGG 
TTCACTGAGG CTGAAATCTT AAAAATATTC TGTGACATCT GTGAAGCTGT TGCGAGGTTA 
CATCAGTGCA AAACTCCAAT CATTCACAGG GATCTCAAGG TTGAAAACAT TTTATTAAAT 
GATCATGGCC ACTATGTACT TTGTGACTTT GGTAGCGCGA CCCACAAAGT TCTTAATCCA 
CAGAAGGATG GAGTTACCAT TGTAGAAGAT GAAATAAAAA AATACACAAC ACTGTCATAC 
AGGGCTCCAG AAATGATAAA TCTTTATGGA GGAAAGTCAA TTACTACCAA GGCTGATATA 
TGGGCACTTG GCTGCTTGTT GTACAAGCTT TGTTTTTTTA CCCTCCCATT TGGGGAGAGT 
CAAGTTGCAA TCTGTGATGG AACATTTACT ATCCCAGATA ATTCCAAATA TTCTTCTAAA 
CTTCACTGTT TAATTCGATA TATGCTTGAA CCAGATGTAG AAAGAAGGCC AGATGTCTAC 
CAAGTTTCAT ATTTTACTTT TAAGATGGCT GGAAGAGAGT GTCCTGTGCC AAACATTTCT 
AACTCTGCTA TCCCATCATT TCTTCCGGAT CCCATGACTG CCAGTGAGGC AGCTGCTAAA 
AAAAGCACGA CGAAGGCCAG AATAACTGAA TCAGTAGGAC CAACAGAAAC TTCAATTGCA 
CCAAGACAGA GACCAAAGGC TGCTTCCAAT ACGTCAAACA CAAATGTGTT GCCAATTCAA 
AACTCTGTAA CTCCAGTGAA AATGTCTGTT TCTGCTGGAA ATATTAGCAA TGGACAACAA 
GCTTTTCAGG GGCCTGGAAC TGGGCATTGC ACACCTCAAT CTCAGGGTAT CAGTCAACAG 
CATAGAGTGC TGCAACAACT TCAACAAGGA GACTTGCGTC TTCAACAGCT TCAACAGTTG 
CATCATCATC ATCAGCAGCA GCAGCAAATG CAGCAAGCAT ATGTACAACA GCATCCACAT 
GTTGCCCAAC AGCAGTATAT ACTTCAGCAC CAGATGATGA TGTTACCGGC ATACCAACAA 
CAAGCTCATT ATGCTGCAAT GATGCAACAA TATCAGCATG CAATTATTCA GCAACAACAG 
CAGCAACAGC AGCAACAGTT TATCACCTCA CCTCAAGAAT TTTCAAAGTC TTTAGCTTTT 
TCGCCAGTTG TGTCAAGCCA ACCTCACACA GCTGCAGATG GATCTTTTAA TAGCACAAGG 
ACTGGCTTAA TAGACACCGA TATCATTGGT GTCCCTTTGC AGAGTGTCAG TAATCCTCCA 
GATATGTCGG GGTGGAATCC ATTTGGAGAA GACAACTTCT CCAAGCTAAC AGAAGATGAA 
CTTCTGGATA GAGAATTTGA CCTTCTTAGA GCAAATAAAC CTGAAGAAAG AACCTGCCTA 
GACACTAAGC CGGAATCTCA TCATTCATCA CACAACCCAG TTCAGGAGGA CCTTTTTGGC 
TCTGTTCCCT TTGTTTCCAC TCCAGGCTCC AGCTTAGAGC ATCCAGAAGA GGCCACAGCA 
GAGTCAGGGG TTGTTGCAGA GAGCCATGGA AAAGCAGAAA AGCAAGGCCA GCTGTCCAAA 
GAACATAAGG GAGGCAAAAA AAGTGGTCCT TCTGGGCAAC AAACCCGAAG AGGAGGAGAG 
GACTCGGACA GTGACTTTGA GTCAGATCCT CCTTCTCCAA AAAGCAGTGA GGAAGAGGAG 
CAGGAAGACG AAGAAGCCTT AAACAGTGAG CACGGGGAGT TTAACGACAG CAATGACACT 
GAGCCAGAAA ATTTTGGGCA GAGACCCCTT CTGATGGATT CTGAGGAAGA GGTTGAGGAT 
GAAGAGGAGG ACAAGCGGAG CTCAGACTCT GATATTGACC TTAGGAAGAG AAAGCAGCCA 
GGAGTCGGCC TGGGAACTGA TCACGTGGTT GGTGGAACTG GGAATCCTGC CAACAACCAA 
ATAATAATGC CACAAGACCC TGGTATGGAG GCAGGAGCCG CCTTAAAGTC AGAAGTGGAT 
GTGTTTGGTG CTGTGCCTTT CCTTAGAACT GAACAGACCA AGACCTCTGC AGAGAACATG 
GACATATTTT CTCAAGCACC ATTTCAGTCA CCCACCACTG AGCAAAATAT GGATGAATTT 
GATGTATTCA CCAAAGCGCC ATTTAATAGA AGTTTATCCA AACCTGGGAA ACCAGGAGAA 
GCAGCACTCT TCCAGTCCCC GCCAGTTTCT CCAGACAGCA TAGATATTTT TGGCTCAATT 
CCTTTCCAGC CACCCCAACA ATTTCCTGAT TCTGGCAATA AAGAAGACAT TTTTGGGCAG 
GTGCCATTTG AAGACTGTTC AACGCCTCAG CAGCAAAAAA CAAAGCAGAG GAGTCTCCAA 
AAACTTTCCT CCAGACAAAG ACGTACAAAA CAAGAAAATA CAAGTGGAAA CGGGAAGCGG 
CACCATGGCA CTCCCACCAG TGGCAGAAAA ACCACAAAAA CGAACTATCG TACACCGGAG 
CGCGTACGCA GACATAAGAA AGTGGGAAGA CGAGATTCTC AGAGCAGCAA TGAGTTTTTG 
AACACGTCAG ACTCAAAAGA GAATATCAGT TTGGCGCCAC CTGATTCGAA AGACAAAGGG 
CCATCGTTGC AGCCTGAAGA AACAATACTG GACCTGTTTG GTGCAAAGCC ATTCCATCCA 
CCAGAGTCTG GCAGACTCCC ACAGCACCAA GGTTTAGGAG ACAACAGAGG AGATCTCAAC 
TTGAGTGGTG GGAGGCCTAG ACAAAGTTCA CTCCATGGTT CGTTTCATGG TGGAGATGGG 
TTAAAAACAG ATGATTTTGG CGCCGTGCCC TTCACAGAAC TTGTAGTACA GAGTGCCCCC 
CAGCAGGCAG CGCAGGTTGA GCTTGATCCT TTTGGAGCAG CTCCTTTTCC CTCTAAACAA 
TAG