VPS35 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS35
Entrez Gene ID	103128562
Full Name	VPS35, retromer complex component
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007539443.1	XP_007539505.1	vacuolar protein sorting-associated protein 35

Pathways from BioSystems
Gene Ontology

The following VPS35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr02931	XM_007539443.1 Latest version!	Erinaceus europaeus VPS35 retromer complex component (VPS35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS35 ( XM_007539443.1 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007539505.1 Erinaceus europaeus VPS35 retromer complex component (VPS35), mRNA.

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CloneID	OEr02931
Clone ID Related Accession (Same CDS sequence)	XM_007539443.1
Accession Version	XM_007539443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	vacuolar protein sorting-associated protein 35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006803956.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACAA CACAGCAGTC ACCTCAGGAT GAGCAGGAGA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAGATG AAGAGATGCC TGGACAAAAA CAAGCTTATG 
GATGCTCTGA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA 
AAGAGCTACT ATGAACTTTA TATGGCTATT TCTGATGAAC TACACTACTT AGAAGTCTAC 
CTAACAGATG AATTTGCTAA AGGAAGAAAA GTGGCAGATC TGTATGAACT TGTACAATAT 
GCTGGAAATA TTATCCCAAG ACTTTATCTG TTGATCACAG TTGGAGTTGT ATATGTCAAG 
TCATTTCCTC AGTCCAGGAA AGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG 
CAACATCCCT TAAGAGGCCT TTTTCTTCGA AACTATCTTC TTCAGTGTAC TAGAAATATC 
TTACCTGATG AGGGAGAGCC AACAGATGAA GAAACAACTG GTGATATCAG TGATTCCATG 
GATTTTGTGC TACTTAACTT TGCAGAAATG AATAAGCTCT GGGTGCGAAT GCAACATCAG 
GGACATAGCC GAGATAGAGA AAAAAGAGAA AGAGAAAGAC AAGAGTTAAG AATTTTAGTG 
GGAACAAATT TGGTGCGCCT CAGTCAACTA GAAGGTGTAA ATGTGGAGCG TTACAAACAG 
ATCGTTTTGA CTGGCATATT GGAGCAAGTT GTAAATTGTA GGGATGCTCT GGCTCAAGAA 
TATCTCATGG AATGCATCAT TCAGGTGTTT CCTGATGAAT TCCATCTTCA AACTTTGAAT 
CCTTTTCTTC GGGCTTGTGC TGAATTACAC CAAAATGTAA ATGTGAAAAA TATAATCATT 
GCTTTAATTG ATAGATTAGC ATTATTTGCT CACCGTGAAG ATGGACCTGG AATTCCAACA 
GATATTAAAC TTTTTGACAT ATTTTCACAG CAGGTTGCTA CAGTAATACA GTCCAGACAA 
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TCATTAATCT TGCTATGAAG 
TGTTACCCAG ATCGTGTAGA TTATGTTGAT AAAGTCCTAG AAACAACAGT GGAAATATTT 
AACAAACTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA 
CTTTTGAAGA TACCTGTTGA CACTTATAAC AATATTTTAA CAGTCTTAAA ATTAAAACAT 
TTTCATCCAC TCTTTGAGTA CTTTGACTAT GAATCTAGAA AGAGCATGAG TTGTTATGTG 
CTCAGTAACG TTTTGGACTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA 
ATGAATTTAG TATCCACATT AATTCAGGAT CAGCCAGATC AACCTATAGA AGACCCTGAT 
CCAGAAGACT TTGCTGATGA GCAGAGCCTT GTGGGCAGAT TCATTCATCT TTTGCGGTCT 
GATGACCCTG ATCAGCAGTA CTTGATTTTA AACACTGCAC GGAAGCATTT TGGAGCTGGT 
GGAAATCAGC GGATTCGTTT CACATTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT 
TTTCGCTACA AAGAGAATTC TAAAGTGGAT GACAAGTGGG AAAAGAAATG CCAGAAGATT 
TTTTCATTTG CTCATCAGAC CATCAGTGCT TTGATCAAAG CAGAGTTGGC AGAATTGCCC 
TTAAGACTCT TTCTTCAAGG AGCTTTAGCT GCTGGAGAAA TTGGTTTTGA AAATCACGAA 
ACAGTAGCAT ATGAATTTAT GTCACAGGCA TTTTCTCTTT ATGAAGATGA AATCAGTGAT 
TCCAAAGCAC AGCTGGCTGC CATCACATTA ATCATTGGTA CTTTTGAAAG GATGAAGTGT 
TTCAGTGAAG AGAATCACGA GCCCTTGAGG ACTCAGTGTG CACTTGCTGC ATCAAAGCTT 
CTTAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CCCATCTTTT TTGGTCTGGC 
AGAAACACTG ACAAAAATGG GGAGGAGCTT CATGGAGGCA AAAGGGTAAT GGAATGCCTA 
AAGAAAGCTC TAAAAATAGC TAATCAATGC ATGGACCCCT CTCTACAAGT GCAACTTTTT 
ATAGAAATTC TGAACAGATA CATCTATTTT TATGAAAAAG AAAATGATGC AGTCACAATT 
CAGGTTTTGA ACCAACTTAT CCAAAAGATT CGAGAAGACC TCCCAAATCT TGAGTCCAGT 
GAAGAAACAG AGCAAATTAA CAAACATTTC CATAATACCC TGGAGCATTT GCGACTGAGG 
CGGGAATCAC CAGAATCTGA GGGGCCAATT TATGAAGGTC TTGTCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007539505.1
CDS	101..2491
Translation	MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPTDIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPIEDPDPEDFADEQSLVGRFIHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLVL

Target ORF information:

RefSeq Version	XM_007539443.1
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus VPS35 retromer complex component (VPS35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007539443.1

ORF Insert Sequence:

ATGCCTACAA CACAGCAGTC ACCTCAGGAT GAGCAGGAGA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAGATG AAGAGATGCC TGGACAAAAA CAAGCTTATG 
GATGCTCTGA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA 
AAGAGCTACT ATGAACTTTA TATGGCTATT TCTGATGAAC TACACTACTT AGAAGTCTAC 
CTAACAGATG AATTTGCTAA AGGAAGAAAA GTGGCAGATC TGTATGAACT TGTACAATAT 
GCTGGAAATA TTATCCCAAG ACTTTATCTG TTGATCACAG TTGGAGTTGT ATATGTCAAG 
TCATTTCCTC AGTCCAGGAA AGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG 
CAACATCCCT TAAGAGGCCT TTTTCTTCGA AACTATCTTC TTCAGTGTAC TAGAAATATC 
TTACCTGATG AGGGAGAGCC AACAGATGAA GAAACAACTG GTGATATCAG TGATTCCATG 
GATTTTGTGC TACTTAACTT TGCAGAAATG AATAAGCTCT GGGTGCGAAT GCAACATCAG 
GGACATAGCC GAGATAGAGA AAAAAGAGAA AGAGAAAGAC AAGAGTTAAG AATTTTAGTG 
GGAACAAATT TGGTGCGCCT CAGTCAACTA GAAGGTGTAA ATGTGGAGCG TTACAAACAG 
ATCGTTTTGA CTGGCATATT GGAGCAAGTT GTAAATTGTA GGGATGCTCT GGCTCAAGAA 
TATCTCATGG AATGCATCAT TCAGGTGTTT CCTGATGAAT TCCATCTTCA AACTTTGAAT 
CCTTTTCTTC GGGCTTGTGC TGAATTACAC CAAAATGTAA ATGTGAAAAA TATAATCATT 
GCTTTAATTG ATAGATTAGC ATTATTTGCT CACCGTGAAG ATGGACCTGG AATTCCAACA 
GATATTAAAC TTTTTGACAT ATTTTCACAG CAGGTTGCTA CAGTAATACA GTCCAGACAA 
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TCATTAATCT TGCTATGAAG 
TGTTACCCAG ATCGTGTAGA TTATGTTGAT AAAGTCCTAG AAACAACAGT GGAAATATTT 
AACAAACTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA 
CTTTTGAAGA TACCTGTTGA CACTTATAAC AATATTTTAA CAGTCTTAAA ATTAAAACAT 
TTTCATCCAC TCTTTGAGTA CTTTGACTAT GAATCTAGAA AGAGCATGAG TTGTTATGTG 
CTCAGTAACG TTTTGGACTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA 
ATGAATTTAG TATCCACATT AATTCAGGAT CAGCCAGATC AACCTATAGA AGACCCTGAT 
CCAGAAGACT TTGCTGATGA GCAGAGCCTT GTGGGCAGAT TCATTCATCT TTTGCGGTCT 
GATGACCCTG ATCAGCAGTA CTTGATTTTA AACACTGCAC GGAAGCATTT TGGAGCTGGT 
GGAAATCAGC GGATTCGTTT CACATTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT 
TTTCGCTACA AAGAGAATTC TAAAGTGGAT GACAAGTGGG AAAAGAAATG CCAGAAGATT 
TTTTCATTTG CTCATCAGAC CATCAGTGCT TTGATCAAAG CAGAGTTGGC AGAATTGCCC 
TTAAGACTCT TTCTTCAAGG AGCTTTAGCT GCTGGAGAAA TTGGTTTTGA AAATCACGAA 
ACAGTAGCAT ATGAATTTAT GTCACAGGCA TTTTCTCTTT ATGAAGATGA AATCAGTGAT 
TCCAAAGCAC AGCTGGCTGC CATCACATTA ATCATTGGTA CTTTTGAAAG GATGAAGTGT 
TTCAGTGAAG AGAATCACGA GCCCTTGAGG ACTCAGTGTG CACTTGCTGC ATCAAAGCTT 
CTTAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CCCATCTTTT TTGGTCTGGC 
AGAAACACTG ACAAAAATGG GGAGGAGCTT CATGGAGGCA AAAGGGTAAT GGAATGCCTA 
AAGAAAGCTC TAAAAATAGC TAATCAATGC ATGGACCCCT CTCTACAAGT GCAACTTTTT 
ATAGAAATTC TGAACAGATA CATCTATTTT TATGAAAAAG AAAATGATGC AGTCACAATT 
CAGGTTTTGA ACCAACTTAT CCAAAAGATT CGAGAAGACC TCCCAAATCT TGAGTCCAGT 
GAAGAAACAG AGCAAATTAA CAAACATTTC CATAATACCC TGGAGCATTT GCGACTGAGG 
CGGGAATCAC CAGAATCTGA GGGGCCAATT TATGAAGGTC TTGTCCTTTA A