NEK1 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEK1
Entrez Gene ID	103117221
Full Name	NIMA related kinase 1
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007527234.2	XP_007527296.1	LOW QUALITY PROTEIN: serine/threonine-protein kinase Nek1

Pathways from BioSystems
Gene Ontology

The following NEK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr15300	XM_007527234.2 Latest version!	Erinaceus europaeus NIMA related kinase 1 (NEK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK1 ( XM_007527234.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007527296.1 Erinaceus europaeus NIMA related kinase 1 (NEK1), mRNA.

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CloneID	OEr15300
Clone ID Related Accession (Same CDS sequence)	XM_007527234.2
Accession Version	XM_007527234.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	LOW QUALITY PROTEIN: serine/threonine-protein kinase Nek1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007527234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAGAAGT ATGTTAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATCCTT 
GTTAAATCTA CAGAAGATGG CAGGCAATAT GTTATCAAGG AAATTAGTAT CTCAAGAATG 
TCCAGTAAAG AAAGGGAAGA GTCAAGGAGA GAAGTTGCAG TATTGGAAAA TATGAAACAT 
CCAAATATTG TCCAGTATAG AGAGTCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTATTTAAA CGAATAAATG CTCAGAAAGG CACTTTTTTC 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCATTGAA ACATGTACAT 
GATAGAAAAA TTCTCCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAAAGATGGG 
ACTGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAACTGGCT 
CGGACTTGCA TAGGTACTCC GTACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTGGGA TGTGTTCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGTAG TATGAAAAAC CTGGTACTGA AGATTATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAAGTTT TATAGCCAAA 
CGCATTGTAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAATCATTT 
CCCAAGTTTG GACCACAGCC AGCTAAAAGA CCAACTTCAG GACAGAGTTT GGCTTCTGTT 
GTGTCTGCTC AAAAAATCAC AAAGCCTTCT GCAAAATATG GAGTATCTTT AGGATATAAG 
AAGTATGTAG ATAAAAAATT ACTTGAGAAG AAACCACTCC AAAAACATAA ACAGGCCCAC 
CAAAGTCCAG TGAAGAAAGT GAATCCTGGA GAAGAAAGGA GGAAAATGTT TGAGGAAGCA 
AAAAAAAGGG GAAGGTTGGA ACTTACTGAA AAAGAAAAGA AACAAAAGGA TCAGATTAGT 
TTAATGAAGG CTGAAGGGAT GAGAAGACTA GAAAAGGAAC GGTTGGAGAG AATAAATAGG 
GCCAGAGAAC AAGGATGGAG AAATGTACTA AGTGCTGGTG GAAGTGGTGA AGTAAAGGCT 
CCCTTTTTTA TCAGTGGAGG ATCTGTGGCT CCACCTTTCT CTCCTCGAGG ACAGTATGAA 
CATTATCATG CTATTTTTGA CCAAATGCAG CAACAAAGAG AAAATAACGA AGCTAAGTGG 
AAAGGAGAAA TGCATGGTCG AAGATTTTAT GAAAGACTTC CTAAAGGGGC TACAAGTCAT 
CATCATAACC CTGATGCTGC TGCTACTAGA GAAAGTTTGA AAAGATTGAA GGCCATGTCT 
TACCAGNGCA ATGCAAACAG GCAAAAGGGA CATATATCTG TGGAAAGAGC TAAACAGGTG 
GAAGAATTCC TACAGAGAAA ACGGGAAGCA ATGCAAAATA AAGCACGAGC TGAAGGACAT 
ATGGGAATCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGTCCAGCTC TTCAAGAGGA 
GGGAAACCAA GAGACAAAGA GGAAGAGGTT TATCTGGCAA GACTGAGACA GATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAAATCAGA GCAAAACTTC GTGGTGAAAA GAAAGAAGCT 
GATAGTTCTG AAGGACAAGG GATTGAAGAG GCTGACATGA GACGTAAAAA AATTGAAATA 
TTTAAGGCCC AAGCAAATAC ACGTGCTGCT ATCTTGAAAG AACAATTAGA ACGAAAAAGA 
AAGGAAGCAT ATGAGAGAGA AAACAAAGTA TGGGAAGAGC ATTTAGTGGC TAAAGGAGTA 
AAGAATTCTG ATGTGCCTCC ACCCTGGGGA CAGCATGATA CAGGTGGTTC TCCATCAAAA 
CAGCTGGTGA AACCTGCTAT TTCTGTGACT TCTGCTTTGA AAGAAGTGGG TATGGACAGA 
AGTTTAACTG ATACCCAGGA AGTCTCAGAA GAAATGCAAA AGACAAATGT TGCTATCTCA 
AGCAAGAGGA AAATACTACG TAGGTTAAAT CAAAATCTTA AAGCTCAGGA AGATGAAAAA 
GGAAAGCAGT ATCATTCTGA TAGATGTGAG ATTATTCATG AAAATGCCAA AGAGCATGAG 
AAGGAAAAAC CTGTTTCGTC TGATCGCAAG AAATGGGAAG CAGGTGGTCA ACTTGTGATT 
CCTCTGGATG ACTTAACACT TGATACATCC TTCTCTGCAA GTGAAAATCA TACCATTGGA 
GAGGTTATTA AGTTGGATCC TAGTGAATCT CCAAGAAGAG TCTGGGGAAA AAGCCCTACA 
GAATCTGTCC TAAAGGTACT AGGAGAAGCT GAACTACAGC CTCAGACAGA ACTGTTAGAA 
AACACAGCCA TTAGAAGTGA AACTTCTCCT GATGAGGAAA AATATAAACC CTTAGTTACT 
GAAGAAGAAA AAATAAAGTG TGTTTCACTT GAAATGAACA TATCAGCTAC TGTCGATTAT 
CCTGTTGTGA CAAGTAGTCC AGAGGTTAAT GAGTTATCTT CACAGATATC ACTGCAACCA 
GATGGAAATT TGGATGAACC TGATGACTTG GAAACAGAAA TTCTACAAGA GCCAAGTAGA 
ACCAATAAAA ATGATAGCTT GCCATGCTTT GTTGATGTAT GGATTAATGA GGTGAAAGAA 
ACACAGGAAA CTGAATTGGA AGACAGGACT GACATTAACG AAAGTGAAGT ACCTGAGGAT 
GGAATTCCAA AGAATGTGAA TCAACTTAAT GATGTACCTG TAGAACCTGA AATAGATGAT 
TCTGAACACT CTAAATGCGA GATAGATAAA CCTGTGCAAC CAGAACCATT TTTCCATCAA 
GTGATTCATT CTGAACACTT GAACTTAGTC TCTCAGATTC AGTCAGTCCT GTGTTTGGCA 
GAAGAATCCT TTCCACTTCG ATCTCGCTCT GATTCACCAC CAAAAACCAA GAGCAAGAAT 
TCTCTGCTAA TTGGACTTTC AACTGGTCTG TTTGATGCAA ACAATCCAAA GATGTTGAGG 
ACATGTTCAC TTCCAGATCT CTCAAAATTA TTTAGAACTC TAATGGATGT ACCCACTGTA 
GGAGATGTAC AGCAAGACAA TCTTGAAATA GATGAAATTG GAGATGAACA CAGTAAAGAA 
GCTCCTTCAG ATTCTGAAGA CATTGTGTTT GAAGAAGCTG ATACAGATTT GCAAGAACTT 
CAGGCTTCCA TGGAGCAGTT ACTTAGGGAA CAACCTGGGG AAGAATATAG TGAGGAGGAA 
GAATCCATCT TAAAGAACAG TGATGTAGAA CAGAATGCAA ATAGGACACA TCTGGCAGAT 
GAAGATGACA ATCCCAGCAG TGAAAGTGCC TTAAACGAAG AATGGCACTC AGATAATAGT 
GATGGTGAGA TTACTAGTGA ATGTGAATAT GATAGTGTCT TTAATCACCT AGAGGAACTG 
AGACTTCAAC TGGAGCAGGA AATGGGCTTT GAAAAGTTCT TTGAGGTTTA TGAGAAAGTG 
AAGGCTATTC ATGAAGATGA AGATGAAAAT ATTGAGGCTT GTTCAACAGT AGTTCAGAAT 
ATTTTGGGAA ATGAACATAA GCATCTTTAT GCCAAGATTC TTCATTTAGT CATGGCAGAT 
GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007527296.1
CDS	201..4010
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEISISRMSSKEREESRREVAVLENMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGTFFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKSFIAKRIVKFLSPQLIAEEFCLKSFPKFGPQPAKRPTSGQSLASVVSAQKITKPSAKYGVSLGYKKYVDKKLLEKKPLQKHKQAHQSPVKKVNPGEERRKMFEEAKKRGRLELTEKEKKQKDQISLMKAEGMRRLEKERLERINRAREQGWRNVLSAGGSGEVKAPFFISGGSVAPPFSPRGQYEHYHAIFDQMQQQRENNEAKWKGEMHGRRFYERLPKGATSHHHNPDAAATRESLKRLKAMSYQnullNANRQKGHISVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRSSSSRGGKPRDKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEADSSEGQGIEEADMRRKKIEIFKAQANTRAAILKEQLERKRKEAYERENKVWEEHLVAKGVKNSDVPPPWGQHDTGGSPSKQLVKPAISVTSALKEVGMDRSLTDTQEVSEEMQKTNVAISSKRKILRRLNQNLKAQEDEKGKQYHSDRCEIIHENAKEHEKEKPVSSDRKKWEAGGQLVIPLDDLTLDTSFSASENHTIGEVIKLDPSESPRRVWGKSPTESVLKVLGEAELQPQTELLENTAIRSETSPDEEKYKPLVTEEEKIKCVSLEMNISATVDYPVVTSSPEVNELSSQISLQPDGNLDEPDDLETEILQEPSRTNKNDSLPCFVDVWINEVKETQETELEDRTDINESEVPEDGIPKNVNQLNDVPVEPEIDDSEHSKCEIDKPVQPEPFFHQVIHSEHLNLVSQIQSVLCLAEESFPLRSRSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVQQDNLEIDEIGDEHSKEAPSDSEDIVFEEADTDLQELQASMEQLLREQPGEEYSEEEESILKNSDVEQNANRTHLADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLQLEQEMGFEKFFEVYEKVKAIHEDEDENIEACSTVVQNILGNEHKHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_007527234.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus NIMA related kinase 1 (NEK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007527234.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATCCTT 
GTTAAATCTA CAGAAGATGG CAGGCAATAT GTTATCAAGG AAATTAGTAT CTCAAGAATG 
TCCAGTAAAG AAAGGGAAGA GTCAAGGAGA GAAGTTGCAG TATTGGAAAA TATGAAACAT 
CCAAATATTG TCCAGTATAG AGAGTCATTT GAAGAAAATG GCTCTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTATTTAAA CGAATAAATG CTCAGAAAGG CACTTTTTTC 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCATTGAA ACATGTACAT 
GATAGAAAAA TTCTCCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAAAGATGGG 
ACTGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAACTGGCT 
CGGACTTGCA TAGGTACTCC GTACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAC 
AACAATAAAA GTGACATTTG GGCTCTGGGA TGTGTTCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGTAG TATGAAAAAC CTGGTACTGA AGATTATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCTTATGAT CTCCGCAGTT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAAGTTT TATAGCCAAA 
CGCATTGTAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAATCATTT 
CCCAAGTTTG GACCACAGCC AGCTAAAAGA CCAACTTCAG GACAGAGTTT GGCTTCTGTT 
GTGTCTGCTC AAAAAATCAC AAAGCCTTCT GCAAAATATG GAGTATCTTT AGGATATAAG 
AAGTATGTAG ATAAAAAATT ACTTGAGAAG AAACCACTCC AAAAACATAA ACAGGCCCAC 
CAAAGTCCAG TGAAGAAAGT GAATCCTGGA GAAGAAAGGA GGAAAATGTT TGAGGAAGCA 
AAAAAAAGGG GAAGGTTGGA ACTTACTGAA AAAGAAAAGA AACAAAAGGA TCAGATTAGT 
TTAATGAAGG CTGAAGGGAT GAGAAGACTA GAAAAGGAAC GGTTGGAGAG AATAAATAGG 
GCCAGAGAAC AAGGATGGAG AAATGTACTA AGTGCTGGTG GAAGTGGTGA AGTAAAGGCT 
CCCTTTTTTA TCAGTGGAGG ATCTGTGGCT CCACCTTTCT CTCCTCGAGG ACAGTATGAA 
CATTATCATG CTATTTTTGA CCAAATGCAG CAACAAAGAG AAAATAACGA AGCTAAGTGG 
AAAGGAGAAA TGCATGGTCG AAGATTTTAT GAAAGACTTC CTAAAGGGGC TACAAGTCAT 
CATCATAACC CTGATGCTGC TGCTACTAGA GAAAGTTTGA AAAGATTGAA GGCCATGTCT 
TACCAGNGCA ATGCAAACAG GCAAAAGGGA CATATATCTG TGGAAAGAGC TAAACAGGTG 
GAAGAATTCC TACAGAGAAA ACGGGAAGCA ATGCAAAATA AAGCACGAGC TGAAGGACAT 
ATGGGAATCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGTCCAGCTC TTCAAGAGGA 
GGGAAACCAA GAGACAAAGA GGAAGAGGTT TATCTGGCAA GACTGAGACA GATAAGATTG 
CAGAATTTCA ATGAGCGCCA ACAAATCAGA GCAAAACTTC GTGGTGAAAA GAAAGAAGCT 
GATAGTTCTG AAGGACAAGG GATTGAAGAG GCTGACATGA GACGTAAAAA AATTGAAATA 
TTTAAGGCCC AAGCAAATAC ACGTGCTGCT ATCTTGAAAG AACAATTAGA ACGAAAAAGA 
AAGGAAGCAT ATGAGAGAGA AAACAAAGTA TGGGAAGAGC ATTTAGTGGC TAAAGGAGTA 
AAGAATTCTG ATGTGCCTCC ACCCTGGGGA CAGCATGATA CAGGTGGTTC TCCATCAAAA 
CAGCTGGTGA AACCTGCTAT TTCTGTGACT TCTGCTTTGA AAGAAGTGGG TATGGACAGA 
AGTTTAACTG ATACCCAGGA AGTCTCAGAA GAAATGCAAA AGACAAATGT TGCTATCTCA 
AGCAAGAGGA AAATACTACG TAGGTTAAAT CAAAATCTTA AAGCTCAGGA AGATGAAAAA 
GGAAAGCAGT ATCATTCTGA TAGATGTGAG ATTATTCATG AAAATGCCAA AGAGCATGAG 
AAGGAAAAAC CTGTTTCGTC TGATCGCAAG AAATGGGAAG CAGGTGGTCA ACTTGTGATT 
CCTCTGGATG ACTTAACACT TGATACATCC TTCTCTGCAA GTGAAAATCA TACCATTGGA 
GAGGTTATTA AGTTGGATCC TAGTGAATCT CCAAGAAGAG TCTGGGGAAA AAGCCCTACA 
GAATCTGTCC TAAAGGTACT AGGAGAAGCT GAACTACAGC CTCAGACAGA ACTGTTAGAA 
AACACAGCCA TTAGAAGTGA AACTTCTCCT GATGAGGAAA AATATAAACC CTTAGTTACT 
GAAGAAGAAA AAATAAAGTG TGTTTCACTT GAAATGAACA TATCAGCTAC TGTCGATTAT 
CCTGTTGTGA CAAGTAGTCC AGAGGTTAAT GAGTTATCTT CACAGATATC ACTGCAACCA 
GATGGAAATT TGGATGAACC TGATGACTTG GAAACAGAAA TTCTACAAGA GCCAAGTAGA 
ACCAATAAAA ATGATAGCTT GCCATGCTTT GTTGATGTAT GGATTAATGA GGTGAAAGAA 
ACACAGGAAA CTGAATTGGA AGACAGGACT GACATTAACG AAAGTGAAGT ACCTGAGGAT 
GGAATTCCAA AGAATGTGAA TCAACTTAAT GATGTACCTG TAGAACCTGA AATAGATGAT 
TCTGAACACT CTAAATGCGA GATAGATAAA CCTGTGCAAC CAGAACCATT TTTCCATCAA 
GTGATTCATT CTGAACACTT GAACTTAGTC TCTCAGATTC AGTCAGTCCT GTGTTTGGCA 
GAAGAATCCT TTCCACTTCG ATCTCGCTCT GATTCACCAC CAAAAACCAA GAGCAAGAAT 
TCTCTGCTAA TTGGACTTTC AACTGGTCTG TTTGATGCAA ACAATCCAAA GATGTTGAGG 
ACATGTTCAC TTCCAGATCT CTCAAAATTA TTTAGAACTC TAATGGATGT ACCCACTGTA 
GGAGATGTAC AGCAAGACAA TCTTGAAATA GATGAAATTG GAGATGAACA CAGTAAAGAA 
GCTCCTTCAG ATTCTGAAGA CATTGTGTTT GAAGAAGCTG ATACAGATTT GCAAGAACTT 
CAGGCTTCCA TGGAGCAGTT ACTTAGGGAA CAACCTGGGG AAGAATATAG TGAGGAGGAA 
GAATCCATCT TAAAGAACAG TGATGTAGAA CAGAATGCAA ATAGGACACA TCTGGCAGAT 
GAAGATGACA ATCCCAGCAG TGAAAGTGCC TTAAACGAAG AATGGCACTC AGATAATAGT 
GATGGTGAGA TTACTAGTGA ATGTGAATAT GATAGTGTCT TTAATCACCT AGAGGAACTG 
AGACTTCAAC TGGAGCAGGA AATGGGCTTT GAAAAGTTCT TTGAGGTTTA TGAGAAAGTG 
AAGGCTATTC ATGAAGATGA AGATGAAAAT ATTGAGGCTT GTTCAACAGT AGTTCAGAAT 
ATTTTGGGAA ATGAACATAA GCATCTTTAT GCCAAGATTC TTCATTTAGT CATGGCAGAT 
GGAGCCTATC AAGAAGATAA TGATGAATAA