WWP1 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	WWP1
Entrez Gene ID	103108421
Full Name	WW domain containing E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016185659.1	XP_016041145.1	NEDD4-like E3 ubiquitin-protein ligase WWP1
XM_007517349.2	XP_007517411.1	NEDD4-like E3 ubiquitin-protein ligase WWP1

Pathways from BioSystems
Gene Ontology

The following WWP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WWP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr05092	XM_016185659.1 Latest version!	Erinaceus europaeus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEr05092	XM_007517349.2 Latest version!	Erinaceus europaeus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WWP1 ( XM_016185659.1 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_016041145.1 Erinaceus europaeus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEr05092
Clone ID Related Accession (Same CDS sequence)	XM_016185659.1 , XM_007517349.2
Accession Version	XM_016185659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006803994.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACTG CTTCTCCAAG GTCTGATACT AGTACTAACC ACAGTGGAAG GTTACAGTTA 
CAGGTAACTG TTTCTAGTGC CAAACTTAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA 
TATACAGAAG TAGTTGTAGA TGGAGAAATT AAGAAAACAT CAAAATCCAG TAGTTCTTCT 
AATCCAAAAT GGGATGAACA GCTACTTGTA AATGTGACCC CACAGACTAC TTTGGAATTT 
CGAGTTTGGA GTCATCACAC TTTAAAAGCA GACGCTTTGT TAGGAAAGGC AACAATAGAT 
TTGAAACAAG CTCTGTTATT ACACAACAGA AAATTGGAAA AAGTGAAAGA ACAGTTAAAA 
CTCTCTATGG AAAACAAGAA TGGCATAGTT CAAACTGGTG AATTAACAGT TGTGCTTGAT 
GGACTTGTAA TTGAACAAGA AAATCTGACA AACTGCAGCT CATCTCCAGC CAGACAAATA 
CAGCAAAATG GTGATGCCTT ACATGAAAAT GTAGAGTCTT CAACAAGGAC AGCTACCAGG 
TTGCCTATTG AAAGTACTAA TGAAGTAGAA AACCACGTAC CTGAAAACAC CCTTGTCCAA 
AACTCATGCT GTTCACATAT TGTTAATGGA GACAACACAT CTTCATCTCC GTCTCAGGTT 
GCTGCCAGAC CCAAAAGTAC ACCAGCTCCA AAACCACTCA CATCTGAGCC TGCCAATGAC 
ACTGTTAATG GAGAATCACC CTCATCTGCA CCAATTGATA ATGCTTCCTC TACGGGTACT 
GTAATAGTAT CTGAGGAAAC CGCCTTGTCT TCAAATTGCA CTACTACTAC TGTTGAAGAT 
CCTCCAGTTC AAGAAATACT GACTTCCTCA GAAAACAGTG AATGTATTCT GTCAAACAGT 
CCAGCATTTG GTTCTGAAGC TAGAAGTACG GTTGATTCTG CCATCTCTAG TTCTGAGAAT 
AATTCTACTT TTGAAGCAGC AAAATCGAGA CAGCCAGATG GTTATGTGGA ATCTTTACGT 
CAGCAGGCTG GAAATGTCAG CACAGAAACT TTACCTTCGG GGTGGGAACA AAGGAAAGAT 
CCTCATGGTA GAACCTATTA TGTGGATCAT AACACTCGAA CGACGACTTG GGAGAGGCCA 
CAACCTTTAC CTCCAGGTTG GGAAAGAAGA GTTGACGATC GTGGAAGGGT TTATTATGTG 
GATCATAATA CCAGAACTAC CACCTGGCAG CGGCCTACCA TGGAGTCTGT TCGAAATTTT 
GAACAGTGGC AGTCACAGCG GAACCAATTG CAGGGAGCTA TGCAACAGTT CAACCAACGA 
TACCTCTACT CGGCTTCAAT GTTAGCTGCA GAAAATGACC CATATGGGCC TTTGCCACCA 
GGCTGGGAAA AAAGAGTTGA TGCCACAGAC AGAGTTTACT TTGTGAATCA CAATACAAAA 
ACCACCCAGT GGGAGGATCC AAGAACACAA GGTTTACAGA ATGAAGAACC CCTTCCAGAA 
GGCTGGGAAA TTAGGTATAC TCGGGAAGGT GTTAGATATT TTGTTGATCA TAATACAAGA 
ACAACAACAT TCAAGGATCC ACGCAATGGG AAGTCATCTG TAACTAAAGG TGGTCCCCAA 
ATTGCTTATG AACGCAGCTT CAGATGGAAA CTTGCTCACT TCCGTTATTT GTGCCAGTCT 
AATGCGCTAC CAAGTCATGT AAAGATCAAT GTATCTCGGC AGACATTGTT TGAAGACTCC 
TTCCAACAGA TTATGGCATT AAAACCCTAT GACTTGAGGA GGAGATTATA TGTAATATTT 
AGAGGTGAAG AAGGACTTGA TTATGGTGGT CTAGCAAGAG AATGGTTTTT CTTGCTTTCA 
CATGAAGTTT TGAACCCCAT GTATTGCTTA TTTGAATATG CTGGCAAGAA CAACTACTGT 
CTACAAATAA ACCCAGCATC AACCATTAAT CCAGACCATC TTTCATATTT CTGTTTCATT 
GGTCGCTTTA TTGCTATGGC ATTATTCCAT GGAAAGTTTA TTGATACTGG CTTCTCTTTA 
CCATTCTACA AACGTATGCT AAGTAAAAAA CTGACTATTA AGGATTTGGA ATCTATTGAT 
ACTGAATTTT ATAACTCTCT TATCTGGATA AGAGACAATA ACATTGAAGA ATGTGGCCTA 
GAAATGTACT TTTCTGTTGA CATGGAGATT TTGGGAAAAG TCACTTCACA TGACCTGAAA 
TTAGGAGGTT CTAATATCCT GGTGACTGAG GAAAACAAAG ATGAATATAT TGGTTTAATG 
ACAGAATGGC GTTTTTCTCG AGGAGTCCAA GAACAGACCA AGGCTTTCCT TGATGGGTTT 
AATGAAGTTG TGCCCCTTCA ATGGCTACAG TACTTTGATG AAAAAGAATT GGAGGTTATG 
CTGTGTGGTA TGCAAGAAGT TGACTTGGCA GATTGGCAGA GAAATACTGT GTATCGACAT 
TATACAAGAA ACAGCAAGCA AATCATTTGG TTTTGGCAGT TTGTAAAAGA GACAGATAAT 
GAAGTAAGAA TGAGGCTATT GCAGTTTGTC ACTGGGACCT GCCGTTTACC TCTCGGAGGA 
TTTGCTGAGC TCATGGGAAG TAATGGGCCT CAAAAATTTT GCATTGAAAA AGTTGGCAAA 
GACACCTGGT TACCAAGAAG CCATACATGT TTTAATCGCT TGGATCTACC ACCATATAAG 
AGTTATGAAC AACTAAAAGA AAAACTTCTT TTTGCAATAG AAGAAACAGA AGGATTCGGA 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016041145.1
CDS	146..2914
Translation	MATASPRSDTSTNHSGRLQLQVTVSSAKLKRKKNWFGTAIYTEVVVDGEIKKTSKSSSSSNPKWDEQLLVNVTPQTTLEFRVWSHHTLKADALLGKATIDLKQALLLHNRKLEKVKEQLKLSMENKNGIVQTGELTVVLDGLVIEQENLTNCSSSPARQIQQNGDALHENVESSTRTATRLPIESTNEVENHVPENTLVQNSCCSHIVNGDNTSSSPSQVAARPKSTPAPKPLTSEPANDTVNGESPSSAPIDNASSTGTVIVSEETALSSNCTTTTVEDPPVQEILTSSENSECILSNSPAFGSEARSTVDSAISSSENNSTFEAAKSRQPDGYVESLRQQAGNVSTETLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDATDRVYFVNHNTKTTQWEDPRTQGLQNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_016185659.1
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016185659.1

ORF Insert Sequence:

ATGGCTACTG CTTCTCCAAG GTCTGATACT AGTACTAACC ACAGTGGAAG GTTACAGTTA 
CAGGTAACTG TTTCTAGTGC CAAACTTAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA 
TATACAGAAG TAGTTGTAGA TGGAGAAATT AAGAAAACAT CAAAATCCAG TAGTTCTTCT 
AATCCAAAAT GGGATGAACA GCTACTTGTA AATGTGACCC CACAGACTAC TTTGGAATTT 
CGAGTTTGGA GTCATCACAC TTTAAAAGCA GACGCTTTGT TAGGAAAGGC AACAATAGAT 
TTGAAACAAG CTCTGTTATT ACACAACAGA AAATTGGAAA AAGTGAAAGA ACAGTTAAAA 
CTCTCTATGG AAAACAAGAA TGGCATAGTT CAAACTGGTG AATTAACAGT TGTGCTTGAT 
GGACTTGTAA TTGAACAAGA AAATCTGACA AACTGCAGCT CATCTCCAGC CAGACAAATA 
CAGCAAAATG GTGATGCCTT ACATGAAAAT GTAGAGTCTT CAACAAGGAC AGCTACCAGG 
TTGCCTATTG AAAGTACTAA TGAAGTAGAA AACCACGTAC CTGAAAACAC CCTTGTCCAA 
AACTCATGCT GTTCACATAT TGTTAATGGA GACAACACAT CTTCATCTCC GTCTCAGGTT 
GCTGCCAGAC CCAAAAGTAC ACCAGCTCCA AAACCACTCA CATCTGAGCC TGCCAATGAC 
ACTGTTAATG GAGAATCACC CTCATCTGCA CCAATTGATA ATGCTTCCTC TACGGGTACT 
GTAATAGTAT CTGAGGAAAC CGCCTTGTCT TCAAATTGCA CTACTACTAC TGTTGAAGAT 
CCTCCAGTTC AAGAAATACT GACTTCCTCA GAAAACAGTG AATGTATTCT GTCAAACAGT 
CCAGCATTTG GTTCTGAAGC TAGAAGTACG GTTGATTCTG CCATCTCTAG TTCTGAGAAT 
AATTCTACTT TTGAAGCAGC AAAATCGAGA CAGCCAGATG GTTATGTGGA ATCTTTACGT 
CAGCAGGCTG GAAATGTCAG CACAGAAACT TTACCTTCGG GGTGGGAACA AAGGAAAGAT 
CCTCATGGTA GAACCTATTA TGTGGATCAT AACACTCGAA CGACGACTTG GGAGAGGCCA 
CAACCTTTAC CTCCAGGTTG GGAAAGAAGA GTTGACGATC GTGGAAGGGT TTATTATGTG 
GATCATAATA CCAGAACTAC CACCTGGCAG CGGCCTACCA TGGAGTCTGT TCGAAATTTT 
GAACAGTGGC AGTCACAGCG GAACCAATTG CAGGGAGCTA TGCAACAGTT CAACCAACGA 
TACCTCTACT CGGCTTCAAT GTTAGCTGCA GAAAATGACC CATATGGGCC TTTGCCACCA 
GGCTGGGAAA AAAGAGTTGA TGCCACAGAC AGAGTTTACT TTGTGAATCA CAATACAAAA 
ACCACCCAGT GGGAGGATCC AAGAACACAA GGTTTACAGA ATGAAGAACC CCTTCCAGAA 
GGCTGGGAAA TTAGGTATAC TCGGGAAGGT GTTAGATATT TTGTTGATCA TAATACAAGA 
ACAACAACAT TCAAGGATCC ACGCAATGGG AAGTCATCTG TAACTAAAGG TGGTCCCCAA 
ATTGCTTATG AACGCAGCTT CAGATGGAAA CTTGCTCACT TCCGTTATTT GTGCCAGTCT 
AATGCGCTAC CAAGTCATGT AAAGATCAAT GTATCTCGGC AGACATTGTT TGAAGACTCC 
TTCCAACAGA TTATGGCATT AAAACCCTAT GACTTGAGGA GGAGATTATA TGTAATATTT 
AGAGGTGAAG AAGGACTTGA TTATGGTGGT CTAGCAAGAG AATGGTTTTT CTTGCTTTCA 
CATGAAGTTT TGAACCCCAT GTATTGCTTA TTTGAATATG CTGGCAAGAA CAACTACTGT 
CTACAAATAA ACCCAGCATC AACCATTAAT CCAGACCATC TTTCATATTT CTGTTTCATT 
GGTCGCTTTA TTGCTATGGC ATTATTCCAT GGAAAGTTTA TTGATACTGG CTTCTCTTTA 
CCATTCTACA AACGTATGCT AAGTAAAAAA CTGACTATTA AGGATTTGGA ATCTATTGAT 
ACTGAATTTT ATAACTCTCT TATCTGGATA AGAGACAATA ACATTGAAGA ATGTGGCCTA 
GAAATGTACT TTTCTGTTGA CATGGAGATT TTGGGAAAAG TCACTTCACA TGACCTGAAA 
TTAGGAGGTT CTAATATCCT GGTGACTGAG GAAAACAAAG ATGAATATAT TGGTTTAATG 
ACAGAATGGC GTTTTTCTCG AGGAGTCCAA GAACAGACCA AGGCTTTCCT TGATGGGTTT 
AATGAAGTTG TGCCCCTTCA ATGGCTACAG TACTTTGATG AAAAAGAATT GGAGGTTATG 
CTGTGTGGTA TGCAAGAAGT TGACTTGGCA GATTGGCAGA GAAATACTGT GTATCGACAT 
TATACAAGAA ACAGCAAGCA AATCATTTGG TTTTGGCAGT TTGTAAAAGA GACAGATAAT 
GAAGTAAGAA TGAGGCTATT GCAGTTTGTC ACTGGGACCT GCCGTTTACC TCTCGGAGGA 
TTTGCTGAGC TCATGGGAAG TAATGGGCCT CAAAAATTTT GCATTGAAAA AGTTGGCAAA 
GACACCTGGT TACCAAGAAG CCATACATGT TTTAATCGCT TGGATCTACC ACCATATAAG 
AGTTATGAAC AACTAAAAGA AAAACTTCTT TTTGCAATAG AAGAAACAGA AGGATTCGGA 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WWP1 ( XM_007517349.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007517411.1 Erinaceus europaeus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEr05092
Clone ID Related Accession (Same CDS sequence)	XM_016185659.1 , XM_007517349.2
Accession Version	XM_007517349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006803994.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007517349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACTG CTTCTCCAAG GTCTGATACT AGTACTAACC ACAGTGGAAG GTTACAGTTA 
CAGGTAACTG TTTCTAGTGC CAAACTTAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA 
TATACAGAAG TAGTTGTAGA TGGAGAAATT AAGAAAACAT CAAAATCCAG TAGTTCTTCT 
AATCCAAAAT GGGATGAACA GCTACTTGTA AATGTGACCC CACAGACTAC TTTGGAATTT 
CGAGTTTGGA GTCATCACAC TTTAAAAGCA GACGCTTTGT TAGGAAAGGC AACAATAGAT 
TTGAAACAAG CTCTGTTATT ACACAACAGA AAATTGGAAA AAGTGAAAGA ACAGTTAAAA 
CTCTCTATGG AAAACAAGAA TGGCATAGTT CAAACTGGTG AATTAACAGT TGTGCTTGAT 
GGACTTGTAA TTGAACAAGA AAATCTGACA AACTGCAGCT CATCTCCAGC CAGACAAATA 
CAGCAAAATG GTGATGCCTT ACATGAAAAT GTAGAGTCTT CAACAAGGAC AGCTACCAGG 
TTGCCTATTG AAAGTACTAA TGAAGTAGAA AACCACGTAC CTGAAAACAC CCTTGTCCAA 
AACTCATGCT GTTCACATAT TGTTAATGGA GACAACACAT CTTCATCTCC GTCTCAGGTT 
GCTGCCAGAC CCAAAAGTAC ACCAGCTCCA AAACCACTCA CATCTGAGCC TGCCAATGAC 
ACTGTTAATG GAGAATCACC CTCATCTGCA CCAATTGATA ATGCTTCCTC TACGGGTACT 
GTAATAGTAT CTGAGGAAAC CGCCTTGTCT TCAAATTGCA CTACTACTAC TGTTGAAGAT 
CCTCCAGTTC AAGAAATACT GACTTCCTCA GAAAACAGTG AATGTATTCT GTCAAACAGT 
CCAGCATTTG GTTCTGAAGC TAGAAGTACG GTTGATTCTG CCATCTCTAG TTCTGAGAAT 
AATTCTACTT TTGAAGCAGC AAAATCGAGA CAGCCAGATG GTTATGTGGA ATCTTTACGT 
CAGCAGGCTG GAAATGTCAG CACAGAAACT TTACCTTCGG GGTGGGAACA AAGGAAAGAT 
CCTCATGGTA GAACCTATTA TGTGGATCAT AACACTCGAA CGACGACTTG GGAGAGGCCA 
CAACCTTTAC CTCCAGGTTG GGAAAGAAGA GTTGACGATC GTGGAAGGGT TTATTATGTG 
GATCATAATA CCAGAACTAC CACCTGGCAG CGGCCTACCA TGGAGTCTGT TCGAAATTTT 
GAACAGTGGC AGTCACAGCG GAACCAATTG CAGGGAGCTA TGCAACAGTT CAACCAACGA 
TACCTCTACT CGGCTTCAAT GTTAGCTGCA GAAAATGACC CATATGGGCC TTTGCCACCA 
GGCTGGGAAA AAAGAGTTGA TGCCACAGAC AGAGTTTACT TTGTGAATCA CAATACAAAA 
ACCACCCAGT GGGAGGATCC AAGAACACAA GGTTTACAGA ATGAAGAACC CCTTCCAGAA 
GGCTGGGAAA TTAGGTATAC TCGGGAAGGT GTTAGATATT TTGTTGATCA TAATACAAGA 
ACAACAACAT TCAAGGATCC ACGCAATGGG AAGTCATCTG TAACTAAAGG TGGTCCCCAA 
ATTGCTTATG AACGCAGCTT CAGATGGAAA CTTGCTCACT TCCGTTATTT GTGCCAGTCT 
AATGCGCTAC CAAGTCATGT AAAGATCAAT GTATCTCGGC AGACATTGTT TGAAGACTCC 
TTCCAACAGA TTATGGCATT AAAACCCTAT GACTTGAGGA GGAGATTATA TGTAATATTT 
AGAGGTGAAG AAGGACTTGA TTATGGTGGT CTAGCAAGAG AATGGTTTTT CTTGCTTTCA 
CATGAAGTTT TGAACCCCAT GTATTGCTTA TTTGAATATG CTGGCAAGAA CAACTACTGT 
CTACAAATAA ACCCAGCATC AACCATTAAT CCAGACCATC TTTCATATTT CTGTTTCATT 
GGTCGCTTTA TTGCTATGGC ATTATTCCAT GGAAAGTTTA TTGATACTGG CTTCTCTTTA 
CCATTCTACA AACGTATGCT AAGTAAAAAA CTGACTATTA AGGATTTGGA ATCTATTGAT 
ACTGAATTTT ATAACTCTCT TATCTGGATA AGAGACAATA ACATTGAAGA ATGTGGCCTA 
GAAATGTACT TTTCTGTTGA CATGGAGATT TTGGGAAAAG TCACTTCACA TGACCTGAAA 
TTAGGAGGTT CTAATATCCT GGTGACTGAG GAAAACAAAG ATGAATATAT TGGTTTAATG 
ACAGAATGGC GTTTTTCTCG AGGAGTCCAA GAACAGACCA AGGCTTTCCT TGATGGGTTT 
AATGAAGTTG TGCCCCTTCA ATGGCTACAG TACTTTGATG AAAAAGAATT GGAGGTTATG 
CTGTGTGGTA TGCAAGAAGT TGACTTGGCA GATTGGCAGA GAAATACTGT GTATCGACAT 
TATACAAGAA ACAGCAAGCA AATCATTTGG TTTTGGCAGT TTGTAAAAGA GACAGATAAT 
GAAGTAAGAA TGAGGCTATT GCAGTTTGTC ACTGGGACCT GCCGTTTACC TCTCGGAGGA 
TTTGCTGAGC TCATGGGAAG TAATGGGCCT CAAAAATTTT GCATTGAAAA AGTTGGCAAA 
GACACCTGGT TACCAAGAAG CCATACATGT TTTAATCGCT TGGATCTACC ACCATATAAG 
AGTTATGAAC AACTAAAAGA AAAACTTCTT TTTGCAATAG AAGAAACAGA AGGATTCGGA 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007517411.1
CDS	253..3021
Translation	MATASPRSDTSTNHSGRLQLQVTVSSAKLKRKKNWFGTAIYTEVVVDGEIKKTSKSSSSSNPKWDEQLLVNVTPQTTLEFRVWSHHTLKADALLGKATIDLKQALLLHNRKLEKVKEQLKLSMENKNGIVQTGELTVVLDGLVIEQENLTNCSSSPARQIQQNGDALHENVESSTRTATRLPIESTNEVENHVPENTLVQNSCCSHIVNGDNTSSSPSQVAARPKSTPAPKPLTSEPANDTVNGESPSSAPIDNASSTGTVIVSEETALSSNCTTTTVEDPPVQEILTSSENSECILSNSPAFGSEARSTVDSAISSSENNSTFEAAKSRQPDGYVESLRQQAGNVSTETLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDATDRVYFVNHNTKTTQWEDPRTQGLQNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_007517349.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007517349.2

ORF Insert Sequence:

ATGGCTACTG CTTCTCCAAG GTCTGATACT AGTACTAACC ACAGTGGAAG GTTACAGTTA 
CAGGTAACTG TTTCTAGTGC CAAACTTAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA 
TATACAGAAG TAGTTGTAGA TGGAGAAATT AAGAAAACAT CAAAATCCAG TAGTTCTTCT 
AATCCAAAAT GGGATGAACA GCTACTTGTA AATGTGACCC CACAGACTAC TTTGGAATTT 
CGAGTTTGGA GTCATCACAC TTTAAAAGCA GACGCTTTGT TAGGAAAGGC AACAATAGAT 
TTGAAACAAG CTCTGTTATT ACACAACAGA AAATTGGAAA AAGTGAAAGA ACAGTTAAAA 
CTCTCTATGG AAAACAAGAA TGGCATAGTT CAAACTGGTG AATTAACAGT TGTGCTTGAT 
GGACTTGTAA TTGAACAAGA AAATCTGACA AACTGCAGCT CATCTCCAGC CAGACAAATA 
CAGCAAAATG GTGATGCCTT ACATGAAAAT GTAGAGTCTT CAACAAGGAC AGCTACCAGG 
TTGCCTATTG AAAGTACTAA TGAAGTAGAA AACCACGTAC CTGAAAACAC CCTTGTCCAA 
AACTCATGCT GTTCACATAT TGTTAATGGA GACAACACAT CTTCATCTCC GTCTCAGGTT 
GCTGCCAGAC CCAAAAGTAC ACCAGCTCCA AAACCACTCA CATCTGAGCC TGCCAATGAC 
ACTGTTAATG GAGAATCACC CTCATCTGCA CCAATTGATA ATGCTTCCTC TACGGGTACT 
GTAATAGTAT CTGAGGAAAC CGCCTTGTCT TCAAATTGCA CTACTACTAC TGTTGAAGAT 
CCTCCAGTTC AAGAAATACT GACTTCCTCA GAAAACAGTG AATGTATTCT GTCAAACAGT 
CCAGCATTTG GTTCTGAAGC TAGAAGTACG GTTGATTCTG CCATCTCTAG TTCTGAGAAT 
AATTCTACTT TTGAAGCAGC AAAATCGAGA CAGCCAGATG GTTATGTGGA ATCTTTACGT 
CAGCAGGCTG GAAATGTCAG CACAGAAACT TTACCTTCGG GGTGGGAACA AAGGAAAGAT 
CCTCATGGTA GAACCTATTA TGTGGATCAT AACACTCGAA CGACGACTTG GGAGAGGCCA 
CAACCTTTAC CTCCAGGTTG GGAAAGAAGA GTTGACGATC GTGGAAGGGT TTATTATGTG 
GATCATAATA CCAGAACTAC CACCTGGCAG CGGCCTACCA TGGAGTCTGT TCGAAATTTT 
GAACAGTGGC AGTCACAGCG GAACCAATTG CAGGGAGCTA TGCAACAGTT CAACCAACGA 
TACCTCTACT CGGCTTCAAT GTTAGCTGCA GAAAATGACC CATATGGGCC TTTGCCACCA 
GGCTGGGAAA AAAGAGTTGA TGCCACAGAC AGAGTTTACT TTGTGAATCA CAATACAAAA 
ACCACCCAGT GGGAGGATCC AAGAACACAA GGTTTACAGA ATGAAGAACC CCTTCCAGAA 
GGCTGGGAAA TTAGGTATAC TCGGGAAGGT GTTAGATATT TTGTTGATCA TAATACAAGA 
ACAACAACAT TCAAGGATCC ACGCAATGGG AAGTCATCTG TAACTAAAGG TGGTCCCCAA 
ATTGCTTATG AACGCAGCTT CAGATGGAAA CTTGCTCACT TCCGTTATTT GTGCCAGTCT 
AATGCGCTAC CAAGTCATGT AAAGATCAAT GTATCTCGGC AGACATTGTT TGAAGACTCC 
TTCCAACAGA TTATGGCATT AAAACCCTAT GACTTGAGGA GGAGATTATA TGTAATATTT 
AGAGGTGAAG AAGGACTTGA TTATGGTGGT CTAGCAAGAG AATGGTTTTT CTTGCTTTCA 
CATGAAGTTT TGAACCCCAT GTATTGCTTA TTTGAATATG CTGGCAAGAA CAACTACTGT 
CTACAAATAA ACCCAGCATC AACCATTAAT CCAGACCATC TTTCATATTT CTGTTTCATT 
GGTCGCTTTA TTGCTATGGC ATTATTCCAT GGAAAGTTTA TTGATACTGG CTTCTCTTTA 
CCATTCTACA AACGTATGCT AAGTAAAAAA CTGACTATTA AGGATTTGGA ATCTATTGAT 
ACTGAATTTT ATAACTCTCT TATCTGGATA AGAGACAATA ACATTGAAGA ATGTGGCCTA 
GAAATGTACT TTTCTGTTGA CATGGAGATT TTGGGAAAAG TCACTTCACA TGACCTGAAA 
TTAGGAGGTT CTAATATCCT GGTGACTGAG GAAAACAAAG ATGAATATAT TGGTTTAATG 
ACAGAATGGC GTTTTTCTCG AGGAGTCCAA GAACAGACCA AGGCTTTCCT TGATGGGTTT 
AATGAAGTTG TGCCCCTTCA ATGGCTACAG TACTTTGATG AAAAAGAATT GGAGGTTATG 
CTGTGTGGTA TGCAAGAAGT TGACTTGGCA GATTGGCAGA GAAATACTGT GTATCGACAT 
TATACAAGAA ACAGCAAGCA AATCATTTGG TTTTGGCAGT TTGTAAAAGA GACAGATAAT 
GAAGTAAGAA TGAGGCTATT GCAGTTTGTC ACTGGGACCT GCCGTTTACC TCTCGGAGGA 
TTTGCTGAGC TCATGGGAAG TAATGGGCCT CAAAAATTTT GCATTGAAAA AGTTGGCAAA 
GACACCTGGT TACCAAGAAG CCATACATGT TTTAATCGCT TGGATCTACC ACCATATAAG 
AGTTATGAAC AACTAAAAGA AAAACTTCTT TTTGCAATAG AAGAAACAGA AGGATTCGGA 
CAAGAATGA