IL1B cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL1B
Entrez Gene ID	100034237
Full Name	interleukin 1, beta
Synonyms	IL-1B
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001082526.1	NP_001075995.1	interleukin-1 beta

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Annotation of the Protein Coding Regions of the Equine Genome.
Hestand MS, Kalbfleisch TS, Coleman SJ, Zeng Z, Liu J, Orlando L, MacLeod JN
PloS one10(6)e0124375(2015)

Interleukin-1? inhibits synthesis of 5-lipooxygenase in lipopolysaccharide-stimulated equine whole blood.
Mangal D, Uboh CE, Jiang Z, Soma LR
Prostaglandins & other lipid mediators1089-22(2014 Jan)

Effects of in vitro exposure to autologous blood and serum on expression of interleukin-8, interleukin-1?, and chemokine (C-X-C motif) ligand 2 in equine primary bronchial epithelial cell cultures.
Ainsworth DM, Reyner CL
American journal of veterinary research73(2)296-301(2012 Feb)

Effects of in vitro exposure to hay dust on expression of interleukin-23, -17, -8, and -1beta and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells from horses susceptible to recurrent airway obstruction.
Reyner CL, Wagner B, Young JC, Ainsworth DM
American journal of veterinary research70(10)1277-83(2009 Oct)

Influence of different semen extenders and seminal plasma on PMN migration and on expression of IL-1beta, IL-6, TNF-alpha and COX-2 mRNA in the equine endometrium.
Palm F, Walter I, Budik S, Kolodziejek J, Nowotny N, Aurich C
Theriogenology70(5)843-51(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased PGE2 production led to reduction in 5-LO products in LPS-treated equine whole blood via IL-1b.
Title: Interleukin-1? inhibits synthesis of 5-lipooxygenase in lipopolysaccharide-stimulated equine whole blood.

Results suggested that chemokine expression by cultured equine BECs following exposure to pulmonary hemorrhage conditions may contribute to the development of inflammatory airway disease in horses.
Title: Effects of in vitro exposure to autologous blood and serum on expression of interleukin-8, interleukin-1?, and chemokine (C-X-C motif) ligand 2 in equine primary bronchial epithelial cell cultures.

The acute pulmonary neutrophilia characteristic of recurrent airway obstruction was not associated with an increase in expression of chemokines in pulmonary mononuclear cells from disease-susceptible horses.
Title: Effects of in vitro exposure to hay dust on expression of interleukin-23, -17, -8, and -1beta and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells from horses susceptible to recurrent airway obstruction.

The effects of semen extender and seminal plasma on the expression of inflammatory modulators in the endometrium of mares are reported.
Title: Influence of different semen extenders and seminal plasma on PMN migration and on expression of IL-1beta, IL-6, TNF-alpha and COX-2 mRNA in the equine endometrium.

This study examined effects of in vitro exposure to solutions of hay dust, lipopolysaccharides, or beta-glucan on cytokine expression in pulmonary mononuclear cells isolated from healthy horses and horses with recurrent airway obstruction.
Title: Effects of in vitro exposure to hay dust on expression of interleukin-17, -23, -8, and -1beta and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells isolated from horses chronically affected with recurrent airway disease.

The following IL1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHb00078	NM_001082526.1 Latest version!	Equus caballus interleukin 1, beta (IL1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL1B ( NM_001082526.1 ) cDNA ORF clone, Equus caballus(horse) -> NP_001075995.1 Equus caballus interleukin 1, beta (IL1B), mRNA.

Price & Availability↑

CloneID	OHb00078
Clone ID Related Accession (Same CDS sequence)	NM_001082526.1
Accession Version	NM_001082526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Equus caballus(horse)
Product	interleukin-1 beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U92481.1. ##Evidence-Data-START## Transcript exon combination :: U92481.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SRS332426, SRS332427 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCAG TACCCGACAC CAGTGACATG ATGACTTACT GCAGCGGCAA TGAGAATGAC 
CTGTTCTTTG AGGAGGATGG CCCAAAACAG ATGAAGGGCA GCTTCCAAGA CCTGGACCTC 
AGCTCCATGG GCAATGGGGG CATCCAGCTT CAATTCTCCC ACCAACTTTA CAACAAGACT 
TTCAAGCATG TCGTGTCAAT CATTGTGGCT ATGGAGAAGC TGAAGAAGAT ACCCGTTCCC 
TGCTCACAGG CCTTCCAGGA TGATGACTTG AGGAGCCTCT TTTCTGTCAT CTTTGAAGAA 
GAACCCATCA TCTGTGACAA CTGGGATGAT GATTATGTGT GTGATGCAGC TGTGCATTCA 
GTGAACTGCA GACTCCGGGA CATATACCAT AAATCCCTGG TGCTGTCCGG TGCATGTGAG 
CTGCAGGCTG TCCACCTCAA CGGAGAGAAT ACAAACCAAC AAGTGGTGTT CTGCATGAGC 
TTTGTGCAAG GAGAAGAAGA GACTGACAAG ATACCTGTGG CCTTGGGCCT CAAGGAAAAG 
AACCTGTACC TGTCTTGTGG GATGAAAGAT GGGAAGCCCA CCCTACAGCT GGAGACAGTA 
GACCCCAATA CTTACCCAAA GAGGAAAATG GAAAAGCGAT TTGTCTTCAA CAAGATGGAA 
ATCAAGGGCA ACGTGGAATT TGAGTCTGCA ATGTACCCCA ACTGGTACAT CAGCACCTCT 
CAAGCAGAAA AAAAGCCTGT CTTCCTAGGA AATACCAGAG GCGGCCGGGA CATAACTGAC 
TTCATCATGG AAATCACCTC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075995.1
CDS	62..868
Translation	MAAVPDTSDMMTYCSGNENDLFFEEDGPKQMKGSFQDLDLSSMGNGGIQLQFSHQLYNKTFKHVVSIIVAMEKLKKIPVPCSQAFQDDDLRSLFSVIFEEEPIICDNWDDDYVCDAAVHSVNCRLRDIYHKSLVLSGACELQAVHLNGENTNQQVVFCMSFVQGEEETDKIPVALGLKEKNLYLSCGMKDGKPTLQLETVDPNTYPKRKMEKRFVFNKMEIKGNVEFESAMYPNWYISTSQAEKKPVFLGNTRGGRDITDFIMEITSA

Target ORF information:

RefSeq Version	NM_001082526.1
Organism	Equus caballus(horse)
Definition	Equus caballus interleukin 1, beta (IL1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082526.1

ORF Insert Sequence:

ATGGCAGCAG TACCCGACAC CAGTGACATG ATGACTTACT GCAGCGGCAA TGAGAATGAC 
CTGTTCTTTG AGGAGGATGG CCCAAAACAG ATGAAGGGCA GCTTCCAAGA CCTGGACCTC 
AGCTCCATGG GCAATGGGGG CATCCAGCTT CAATTCTCCC ACCAACTTTA CAACAAGACT 
TTCAAGCATG TCGTGTCAAT CATTGTGGCT ATGGAGAAGC TGAAGAAGAT ACCCGTTCCC 
TGCTCACAGG CCTTCCAGGA TGATGACTTG AGGAGCCTCT TTTCTGTCAT CTTTGAAGAA 
GAACCCATCA TCTGTGACAA CTGGGATGAT GATTATGTGT GTGATGCAGC TGTGCATTCA 
GTGAACTGCA GACTCCGGGA CATATACCAT AAATCCCTGG TGCTGTCCGG TGCATGTGAG 
CTGCAGGCTG TCCACCTCAA CGGAGAGAAT ACAAACCAAC AAGTGGTGTT CTGCATGAGC 
TTTGTGCAAG GAGAAGAAGA GACTGACAAG ATACCTGTGG CCTTGGGCCT CAAGGAAAAG 
AACCTGTACC TGTCTTGTGG GATGAAAGAT GGGAAGCCCA CCCTACAGCT GGAGACAGTA 
GACCCCAATA CTTACCCAAA GAGGAAAATG GAAAAGCGAT TTGTCTTCAA CAAGATGGAA 
ATCAAGGGCA ACGTGGAATT TGAGTCTGCA ATGTACCCCA ACTGGTACAT CAGCACCTCT 
CAAGCAGAAA AAAAGCCTGT CTTCCTAGGA AATACCAGAG GCGGCCGGGA CATAACTGAC 
TTCATCATGG AAATCACCTC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Annotation of the Protein Coding Regions of the Equine Genome.
PloS one10(6)e0124375(2015)
Hestand MS,Kalbfleisch TS,Coleman SJ,Zeng Z,Liu J,Orlando L,MacLeod JN

Interleukin-1? inhibits synthesis of 5-lipooxygenase in lipopolysaccharide-stimulated equine whole blood.
Prostaglandins & other lipid mediators1089-22(2014 Jan)
Mangal D,Uboh CE,Jiang Z,Soma LR

Effects of in vitro exposure to autologous blood and serum on expression of interleukin-8, interleukin-1?, and chemokine (C-X-C motif) ligand 2 in equine primary bronchial epithelial cell cultures.
American journal of veterinary research73(2)296-301(2012 Feb)
Ainsworth DM,Reyner CL

Effects of in vitro exposure to hay dust on expression of interleukin-23, -17, -8, and -1beta and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells from horses susceptible to recurrent airway obstruction.
American journal of veterinary research70(10)1277-83(2009 Oct)
Reyner CL,Wagner B,Young JC,Ainsworth DM

Influence of different semen extenders and seminal plasma on PMN migration and on expression of IL-1beta, IL-6, TNF-alpha and COX-2 mRNA in the equine endometrium.
Theriogenology70(5)843-51(2008 Sep)
Palm F,Walter I,Budik S,Kolodziejek J,Nowotny N,Aurich C