COMP cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	COMP
Entrez Gene ID	100033911
Full Name	cartilage oligomeric matrix protein
General protein information	Preferred Names cartilage oligomeric matrix protein Names cartilage oligomeric matrix protein
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081856.1	NP_001075325.1	cartilage oligomeric matrix protein precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Annotation of the Protein Coding Regions of the Equine Genome.
Hestand MS, Kalbfleisch TS, Coleman SJ, Zeng Z, Liu J, Orlando L, MacLeod JN
PloS one10(6)e0124375(2015)

Proteomic analysis of tendon extracellular matrix reveals disease stage-specific fragmentation and differential cleavage of COMP (cartilage oligomeric matrix protein).
Dakin SG, Smith RK, Heineg?rd D, ?nnerfjord P, Khabut A, Dudhia J
The Journal of biological chemistry289(8)4919-27(2014 Feb)

Immunolocalization of collagens (I and III) and cartilage oligomeric matrix protein in the normal and injured equine superficial digital flexor tendon.
S?dersten F, Hultenby K, Heineg?rd D, Johnston C, Ekman S
Connective tissue research54(1)62-9(2013)

Increased cartilage oligomeric matrix protein concentrations in equine digital flexor tendon sheath synovial fluid predicts intrathecal tendon damage.
Smith MR, Wright IM, Minshall GJ, Dudhia J, Verheyen K, Heineg?rd D, Smith RK
Veterinary surgery : VS40(1)54-8(2011 Jan)

A preliminary study on the effect of wounding on transforming growth factor-?1 and cartilage oligomeric matrix protein concentrations in the skin of horses.
Dart AJ, Dart CM, Dudhia J, Perkins N, Canfield P, Smith RK
Veterinary surgery : VS40(1)59-65(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The aims of this study were to investigate the proteomic composition of injured tendons during early and late disease stages to identify disease-specific cleavage patterns of the extracellular matrix protein cartilage oligomeric matrix protein (COMP).
Title: Proteomic analysis of tendon extracellular matrix reveals disease stage-specific fragmentation and differential cleavage of COMP (cartilage oligomeric matrix protein).

The present results suggest that not only type III collagen but also cartilage oligomeric matrix protein is involved in the repair and remodeling processes of the digital flexor tendon.
Title: Immunolocalization of collagens (I and III) and cartilage oligomeric matrix protein in the normal and injured equine superficial digital flexor tendon.

COMP concentrations in digital flexor tendon sheath synovial fluid were significantly greater than those in normal horses with noninfected tenosynovitis caused by intrathecal tendon/ligament tearing, but not by other lesions.
Title: Increased cartilage oligomeric matrix protein concentrations in equine digital flexor tendon sheath synovial fluid predicts intrathecal tendon damage.

Within the limitations of the study design, production of COMP during healing of skin wounds does not appear to be influenced by wound type or anatomic site, nor does it appear to be correlated with TGF-beta1 concentrations.
Title: A preliminary study on the effect of wounding on transforming growth factor-?1 and cartilage oligomeric matrix protein concentrations in the skin of horses.

The present study indicates that dynamic in vivo compression at high load and frequency lowers matrix content of COMP in the articular cartilage of the third carpal bone.
Title: Ultrastructural immunolocalization of cartilage oligomeric matrix protein (COMP) in the articular cartilage on the equine third carpal bone in trained and untrained horses.

The following COMP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COMP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHb00101	NM_001081856.1 Latest version!	Equus caballus cartilage oligomeric matrix protein (COMP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COMP ( NM_001081856.1 ) cDNA ORF clone, Equus caballus(horse) -> NP_001075325.1 Equus caballus cartilage oligomeric matrix protein (COMP), mRNA.

Price & Availability↑

CloneID	OHb00101
Clone ID Related Accession (Same CDS sequence)	NM_001081856.1
Accession Version	NM_001081856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-28
Organism	Equus caballus(horse)
Product	cartilage oligomeric matrix protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF325902.1. ##Evidence-Data-START## Transcript exon combination :: AF325902.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2743238, SAMN05591755 [ECO:0000348] ##Evidence-Data-END##

ATGGTTCTCT CCGCCGCCCC CGTTCTCCTG CTCGCCCTGG CCGCCCTCGT GTCCAGCCAG 
GGGCAGACCC CGCTGGGTAC AGAACTGGGC CCACAGATGC TGCGCGAACT GCAAGAGACC 
AACGCGGCGC TGCAGGACGT GCGGGAGCTG CTGCGGCAGC AGGTCAAGGA GATCACGTTC 
CTGAAAAACA CGGTGATGGA GTGTGACGCG TGCGGGATGC AGCCTGCGCG CACCCCCCGC 
GTGAGCGTGC GGCCCCTAGC CCAGTGCGCG CCGGGCTCCT GCTTCCCTGG CGTGGCTTGT 
ACCCAGACGG CGAGCGGCGC GCGCTGCGGA CCCTGCCCCG CGGGCTTCAC GGGCAACGGC 
CCATACTGTG CCGACGTCAA CGAGTGCAAC GCCAATCCCT GCTTCCCTCG CGTCCGCTGC 
ATCAATACCA GCCCCGGTTT CCGCTGCGAG GCTTGCCCGC CCGGGTACAG CGGCCCCACC 
CACGAGGGCG TGGGGATGGC CTTTGCCAAG GCCAACAAGC AGGTTTGCAC GGATATTGAC 
GAGTGTGAGA CCGGGCAGCA TAACTGCGTC CCCAACTCCG TGTGCATCAA CACCCAGGGC 
TCCTTCCAGT GCGGCCCGTG CCAGCCCGGC TTCGTAGGCG ACCAGGCATC AGGCTGCCGT 
CCGCGCGCAC AGCGCTTCTG CCCCGACGGC ACGCCCAGCC CGTGCCACGA GAAGGCCGAC 
TGCGTCCTGG AGCGCGATGG CTCGCGATCG TGCGTGTGCG CCGTCGGCTG GGCCGGCAAC 
GGGCTCCTGT GTGGCCGCGA CACGGACTTG GACGGCTTCC CGGACGAGAA GCTGCGCTGC 
TCGGAGCGCC AGTGTCGCAA GGATAACTGC GTGACGGTAC CCAACTCAGG ACAGGAGGAC 
GCGGATCGCG ACGGCATCGG AGACGCCTGC GACACGGACG CCGACGGAGA CGGAGTCCCC 
AACGAGGGGG ACAACTGCCC GCTGGTGCGG AACCCAGACC AGCGTAACAC GGACGGCGAC 
AAGTGGGGCG ATGCATGCGA CAACTGCCGG TCCCAGAAGA ACGATGACCA GAAGGACACA 
GATCAGGACG GCCGAGGCGA CGCCTGCGAC GATGACATCG ACGGCGACCG GATCCGAAAT 
GCGGTGGACA ACTGCCCCAG GGTGCCCAAC TCAGACCAGA AAGACAGTGA TGGCGATGGT 
ATAGGGGATG TCTGTGACAA CTGTCCCCAG AAGAGCAACC CAGACCAGAG GGACGTGGAC 
CACGACTTCG TGGGAGACGC TTGTGACAGC GACCAAGACA AGGATGGGGA TGGGCACCAG 
GACTCTCGGG ACAATTGCCC CACAGTGCCC AACAGCGCCC AGCAGGACTC AGACAGCGAT 
GGTCAGGGTG ACGCCTGCGA CGAGGATGAC GACAACGACG GGGTCCCCGA CAGTCGGGAC 
AACTGCCGCC TGGTGCCCAA CCCGGGCCAG GAAGACGCTG ACCGGGACGG TGTGGGCGAC 
GTGTGCCAGG GCGACTTCGA CGCAGACAAG GTGGTGGACA AGATTGATGT GTGTCCGGAG 
AACGCCGAAG TCACCCTCAC CGACTTCCGG GCCTTCCAGA CGGTTGTGTT GGACCCCGAG 
GGCGACGCGC AAATAGACCC CAACTGGGTG GTGCTCAACC AGGGGATGGA GATCGTGCAA 
ACAATGAACA GCGACCCTGG CCTGGCTGTG GGTTACACGG CCTTCAATGG CGTGGACTTC 
GAAGGCACGT TCCACGTGAA TACGGTCACA GATGACGACT ACGCGGGCTT CATCTTTGGC 
TACCAGGACA GCTCTAGCTT CTACGTGGTC ATGTGGAAGC AGATGGAGCA GACGTATTGG 
CAGGCGAACC CCTTCCGAGC CGTAGCCGAG CCCGGCATCC AGCTGAAGGC CGTGAAGTCC 
TCCACAGGCC CTGGGGAGCA GCTGCGGAAT GCACTGTGGC ACACGGGGGA CACAGCATCA 
CAGGTGCGGC TGCTATGGAA GGACCCCCGC AACGTGGGCT GGAAGGACAA GACATCCTAC 
CGCTGGTTCC TACAACACCG GCCCCAAGTG GGCTACATCA GAGTGCGGTT CTATGAGGGC 
CCTGAGCTGG TGGCCGACAG CAACGTGGTC TTGGACACGA CCATGCGGGG CGGCCGCCTA 
GGAGTCTTCT GCTTCTCCCA GGAGAACATC ATCTGGGCCA ACCTGCGCTA CCGCTGCAAT 
GACACCATCC CCGAGGACTA CGAGATCCAG CGGTTGCTGC AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075325.1
CDS	35..2302
Translation	MVLSAAPVLLLALAALVSSQGQTPLGTELGPQMLRELQETNAALQDVRELLRQQVKEITFLKNTVMECDACGMQPARTPRVSVRPLAQCAPGSCFPGVACTQTASGARCGPCPAGFTGNGPYCADVNECNANPCFPRVRCINTSPGFRCEACPPGYSGPTHEGVGMAFAKANKQVCTDIDECETGQHNCVPNSVCINTQGSFQCGPCQPGFVGDQASGCRPRAQRFCPDGTPSPCHEKADCVLERDGSRSCVCAVGWAGNGLLCGRDTDLDGFPDEKLRCSERQCRKDNCVTVPNSGQEDADRDGIGDACDTDADGDGVPNEGDNCPLVRNPDQRNTDGDKWGDACDNCRSQKNDDQKDTDQDGRGDACDDDIDGDRIRNAVDNCPRVPNSDQKDSDGDGIGDVCDNCPQKSNPDQRDVDHDFVGDACDSDQDKDGDGHQDSRDNCPTVPNSAQQDSDSDGQGDACDEDDDNDGVPDSRDNCRLVPNPGQEDADRDGVGDVCQGDFDADKVVDKIDVCPENAEVTLTDFRAFQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQMEQTYWQANPFRAVAEPGIQLKAVKSSTGPGEQLRNALWHTGDTASQVRLLWKDPRNVGWKDKTSYRWFLQHRPQVGYIRVRFYEGPELVADSNVVLDTTMRGGRLGVFCFSQENIIWANLRYRCNDTIPEDYEIQRLLQA

Target ORF information:

RefSeq Version	NM_001081856.1
Organism	Equus caballus(horse)
Definition	Equus caballus cartilage oligomeric matrix protein (COMP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081856.1

ORF Insert Sequence:

ATGGTTCTCT CCGCCGCCCC CGTTCTCCTG CTCGCCCTGG CCGCCCTCGT GTCCAGCCAG 
GGGCAGACCC CGCTGGGTAC AGAACTGGGC CCACAGATGC TGCGCGAACT GCAAGAGACC 
AACGCGGCGC TGCAGGACGT GCGGGAGCTG CTGCGGCAGC AGGTCAAGGA GATCACGTTC 
CTGAAAAACA CGGTGATGGA GTGTGACGCG TGCGGGATGC AGCCTGCGCG CACCCCCCGC 
GTGAGCGTGC GGCCCCTAGC CCAGTGCGCG CCGGGCTCCT GCTTCCCTGG CGTGGCTTGT 
ACCCAGACGG CGAGCGGCGC GCGCTGCGGA CCCTGCCCCG CGGGCTTCAC GGGCAACGGC 
CCATACTGTG CCGACGTCAA CGAGTGCAAC GCCAATCCCT GCTTCCCTCG CGTCCGCTGC 
ATCAATACCA GCCCCGGTTT CCGCTGCGAG GCTTGCCCGC CCGGGTACAG CGGCCCCACC 
CACGAGGGCG TGGGGATGGC CTTTGCCAAG GCCAACAAGC AGGTTTGCAC GGATATTGAC 
GAGTGTGAGA CCGGGCAGCA TAACTGCGTC CCCAACTCCG TGTGCATCAA CACCCAGGGC 
TCCTTCCAGT GCGGCCCGTG CCAGCCCGGC TTCGTAGGCG ACCAGGCATC AGGCTGCCGT 
CCGCGCGCAC AGCGCTTCTG CCCCGACGGC ACGCCCAGCC CGTGCCACGA GAAGGCCGAC 
TGCGTCCTGG AGCGCGATGG CTCGCGATCG TGCGTGTGCG CCGTCGGCTG GGCCGGCAAC 
GGGCTCCTGT GTGGCCGCGA CACGGACTTG GACGGCTTCC CGGACGAGAA GCTGCGCTGC 
TCGGAGCGCC AGTGTCGCAA GGATAACTGC GTGACGGTAC CCAACTCAGG ACAGGAGGAC 
GCGGATCGCG ACGGCATCGG AGACGCCTGC GACACGGACG CCGACGGAGA CGGAGTCCCC 
AACGAGGGGG ACAACTGCCC GCTGGTGCGG AACCCAGACC AGCGTAACAC GGACGGCGAC 
AAGTGGGGCG ATGCATGCGA CAACTGCCGG TCCCAGAAGA ACGATGACCA GAAGGACACA 
GATCAGGACG GCCGAGGCGA CGCCTGCGAC GATGACATCG ACGGCGACCG GATCCGAAAT 
GCGGTGGACA ACTGCCCCAG GGTGCCCAAC TCAGACCAGA AAGACAGTGA TGGCGATGGT 
ATAGGGGATG TCTGTGACAA CTGTCCCCAG AAGAGCAACC CAGACCAGAG GGACGTGGAC 
CACGACTTCG TGGGAGACGC TTGTGACAGC GACCAAGACA AGGATGGGGA TGGGCACCAG 
GACTCTCGGG ACAATTGCCC CACAGTGCCC AACAGCGCCC AGCAGGACTC AGACAGCGAT 
GGTCAGGGTG ACGCCTGCGA CGAGGATGAC GACAACGACG GGGTCCCCGA CAGTCGGGAC 
AACTGCCGCC TGGTGCCCAA CCCGGGCCAG GAAGACGCTG ACCGGGACGG TGTGGGCGAC 
GTGTGCCAGG GCGACTTCGA CGCAGACAAG GTGGTGGACA AGATTGATGT GTGTCCGGAG 
AACGCCGAAG TCACCCTCAC CGACTTCCGG GCCTTCCAGA CGGTTGTGTT GGACCCCGAG 
GGCGACGCGC AAATAGACCC CAACTGGGTG GTGCTCAACC AGGGGATGGA GATCGTGCAA 
ACAATGAACA GCGACCCTGG CCTGGCTGTG GGTTACACGG CCTTCAATGG CGTGGACTTC 
GAAGGCACGT TCCACGTGAA TACGGTCACA GATGACGACT ACGCGGGCTT CATCTTTGGC 
TACCAGGACA GCTCTAGCTT CTACGTGGTC ATGTGGAAGC AGATGGAGCA GACGTATTGG 
CAGGCGAACC CCTTCCGAGC CGTAGCCGAG CCCGGCATCC AGCTGAAGGC CGTGAAGTCC 
TCCACAGGCC CTGGGGAGCA GCTGCGGAAT GCACTGTGGC ACACGGGGGA CACAGCATCA 
CAGGTGCGGC TGCTATGGAA GGACCCCCGC AACGTGGGCT GGAAGGACAA GACATCCTAC 
CGCTGGTTCC TACAACACCG GCCCCAAGTG GGCTACATCA GAGTGCGGTT CTATGAGGGC 
CCTGAGCTGG TGGCCGACAG CAACGTGGTC TTGGACACGA CCATGCGGGG CGGCCGCCTA 
GGAGTCTTCT GCTTCTCCCA GGAGAACATC ATCTGGGCCA ACCTGCGCTA CCGCTGCAAT 
GACACCATCC CCGAGGACTA CGAGATCCAG CGGTTGCTGC AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Annotation of the Protein Coding Regions of the Equine Genome.
PloS one10(6)e0124375(2015)
Hestand MS,Kalbfleisch TS,Coleman SJ,Zeng Z,Liu J,Orlando L,MacLeod JN

Proteomic analysis of tendon extracellular matrix reveals disease stage-specific fragmentation and differential cleavage of COMP (cartilage oligomeric matrix protein).
The Journal of biological chemistry289(8)4919-27(2014 Feb)
Dakin SG,Smith RK,Heineg?rd D,?nnerfjord P,Khabut A,Dudhia J

Immunolocalization of collagens (I and III) and cartilage oligomeric matrix protein in the normal and injured equine superficial digital flexor tendon.
Connective tissue research54(1)62-9(2013)
S?dersten F,Hultenby K,Heineg?rd D,Johnston C,Ekman S

Increased cartilage oligomeric matrix protein concentrations in equine digital flexor tendon sheath synovial fluid predicts intrathecal tendon damage.
Veterinary surgery : VS40(1)54-8(2011 Jan)
Smith MR,Wright IM,Minshall GJ,Dudhia J,Verheyen K,Heineg?rd D,Smith RK

A preliminary study on the effect of wounding on transforming growth factor-?1 and cartilage oligomeric matrix protein concentrations in the skin of horses.
Veterinary surgery : VS40(1)59-65(2011 Jan)
Dart AJ,Dart CM,Dudhia J,Perkins N,Canfield P,Smith RK