POLD1 cDNA ORF clone, Equus asinus(ass)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLD1
Entrez Gene ID	106824168
Full Name	DNA polymerase delta 1, catalytic subunit
Gene Type	protein-coding
Organism	Equus asinus(ass)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014830333.1	XP_014685819.1	DNA polymerase delta catalytic subunit isoform X1
XM_014830334.1	XP_014685820.1	DNA polymerase delta catalytic subunit isoform X2

Pathways from BioSystems
Gene Ontology

The following POLD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEq30634	XM_014830333.1 Latest version!	Equus asinus polymerase (DNA directed), delta 1, catalytic subunit (POLD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OEq30635	XM_014830334.1 Latest version!	Equus asinus polymerase (DNA directed), delta 1, catalytic subunit (POLD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLD1 ( XM_014830333.1 ) cDNA ORF clone, Equus asinus(ass) -> XP_014685819.1 Equus asinus polymerase (DNA directed), delta 1, catalytic subunit (POLD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEq30634
Clone ID Related Accession (Same CDS sequence)	XM_014830333.1
Accession Version	XM_014830333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-01
Organism	Equus asinus(ass)
Product	DNA polymerase delta catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014638557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus asinus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCA AGCGGCGGCC AGGCCCGGGG CCTGGGGTGC CCCCGAAGCG GGCCCGGGGG 
GGCCTCTGGG ATGAGGACGA GCCGTCGGGG CCGTCCCAGT TCGAGGAGGA GCTGGCGCTG 
ATGGAGGAGC TGGAGGCGGA GCGCAGGCTG CAGGAGCAGG AGGAGGAGGA GCTGCAGTCG 
GCCCTGGAGG GGGCTGCGCA CGGGCACCTC TCCCAGACAG CCATAGACCC CCGCTGGCTG 
CGGCCCTCCC CACCTCCCCT GGACCCCCAG ACGGAGCCCC TCATCTTCCA GCAGTTGGAG 
ATCGACCATT ATGTGGGCCC AGCGCAGCCC CTGCCTGGGG CACCCACGCC CTCCCCGGGC 
TCCGTGCCCG TGCTCCGTGC CTTCGGGGTC ACCGACGAGG GCGTCTCTGT CTGCTGCCAC 
ATCCACGGCT TCGCACCCTA CTTCTACACC CCGGCGCCCC CTGGTTTCGG GCCCGAGCAC 
CTGAGCGACC TGCAGCGGGA GCTGAATGTG GCCATCAGCC GGGACCAGCG CGGCGGGAAG 
GAGCTGGCGG GGCCAGCTGT GCTGGCTGTA GAGCTGTGCT CCCGGGAGAG CATGTTCGGG 
TACCAAGGGC GTGGCCCCTC CCCGTTTCTG CGCATCACCC TCGCGCTGCC CCGCCTTGCG 
GCCCCCGCCC GCCGCCTCCT GGAGCAGGGC ATCCGTGTGG CAGGCCTGGG CACCCCCAGC 
TTCGCGCCCT ATGAGGCCAA CGTCGACTTT GAGATCCGGT TCATGGTGGA CACGGACATC 
GTCGGCTGCA ACTGGCTGGA GCTCCCGGCC GGGAAATACG TCCTGAGGCC GAAGGGGAAG 
ACCACACTGT GTCAGCTGGA GGCAGACGTG CTGTGGTCGG ACGTGATCAG TCACGCACCA 
GAAGGGCAGT GGCAGCGAAT CGCCCCCCTG CGCGTGCTCA GCTTCGACAT CGAGTGCGCT 
GGCCGCAAAG GCATCTTCCC CGAACCCGAG CGGGACCCTG TCATCCAGAT CTGCTCGCTG 
GGCCTGCGCT GGGGCGAGCC GGAGCCCTTC CTGCGCCTGG CGCTCACCCT GCGGCCCTGT 
GCTCCCATCC TGGGCGCCAA GGTGCAGAGC TACGAGCGGG AGGAGGAGCT GCTGCAGGCC 
TGGTCGGCCT TCATCCGCAC CATGGACCCC GACGTGATCA CCGGCTACAA CATCCAGAAC 
TTCGACCTTC CCTACCTCAT CTCCCGGGCC CAGACCCTCA AGGTGTCGTC CTTCCCCTTC 
CTGGGCCGCG TGTCTGGCCT CCGCTCCAAC ATCCGGGACT CGTCGTTCCA GTCGAAGCAG 
ACCGGCCGGC GGGACACCAA GGTGGTCAGC ATGGTGGGCC GCGTACAGAT GGACATGCTG 
CAGGTGCTGC TGCGGGAGTA CAAGCTCCGC TCCTACACAC TCAACGCCGT GAGCTTCCAC 
TTCCTGGGCG AGCAGAAGGA GGACGTGCAG CACAGCATCA TCACCGACCT GCAGAACGGG 
AACGAGCAGA CGCGCCGCCG CCTGGCCGTG TACTGCCTCA AGGACGCCTT CCTGCCACTG 
CGGCTGCTCG AGCGGCTCAT GGTGCTGGTG AACGCCGTGG AGATGGCGCG TGTCACCGGC 
GTGCCCCTCA GCTACCTGCT CAGCCGTGGC CAGCAGGTCA AGGTCGTGTC CCAGCTGCTG 
CGACAGGCCA TGCGCGAGGG GCTGGTGATG CCCGTGGTGA AGACGGAGGG CGGTGAGGAC 
TACACGGGGG CCACAGTCAT CGAGCCCCTC AAAGGGTACT ACGATGTCCC CATCGCCACC 
CTGGACTTCT CCTCGCTGTA CCCGTCCATC ATGATGGCCC ACAACCTGTG CTACACCACG 
CTCCTGCGCC CCGGGGCCGC CCAGAAACTG GGCCTGACCG AGGATCAGTT CATCAAGACG 
CCCACAGGGG ACGAGTTTGT GAAGACGTCG GTGCGGAAGG GGCTCCTGCC TCAGATCCTG 
GAGAACCTGC TCAGCGCCCG GAAGAGGGCC AAGGCCGAGC TGGCCAAGGA GACAGACCCC 
TTGCGGCGGC AGGTCTTGGA CGGGCGGCAG CTGGCACTCA AAGTGAGCGC CAACTCTGTG 
TACGGCTTCA CAGGCGCCCA GGTGGGCAAG CTGCCATGCC TGGAGATCTC ACAGAGCGTC 
ACCGGGTTCG GGCGCCAGAT GATTGAAAGA ACAAAGCAGC TGGTGGAGTC CAAGTACACG 
ACGGAGAACG GCTACAGCGC GAGCGCCAAG GTGGTGTACG GTGACACCGA CTCCGTCATG 
TGCCGATTCG GCGTCTCCTC GGTGGCGGAG GCCATGGCCC TAGGGCGGGA GGCCGCCGAC 
TGGGTGTCGG GCCACTTCCC CTCGCCCATC CGGCTGGAGT TTGAGAAGGT CTACTTCCCC 
TACCTGCTCA TCAGCAAGAA GCGCTACGCC GGCCTGCTCT TCTCCTCCCG GCCGGATGCC 
CATGACCGCA TGGACTGCAA GGGCCTGGAG GCCGTGCGCA GGGACAACTG CCCCCTAGTG 
GCCAACCTTG TCACCGCCTC GCTGCGCCGC CTGCTCATCG ACCGAGACCC CAATGGCGCC 
GTGGCCCACG CACAGGAGGT CATCTCGGAC CTGCTGTGCA ACCGCATCGA CATCTCCCAG 
CTGGTCATCA CCAAGGAGCT GACCCGCGCG GCCGCCGACT ACGCCGGCAA ACAGGCCCAC 
GTAGAGCTGG CCGAGAGGAT GAGGAAGCGG GACCCCGGGA GCGCGCCCAG CCTGGGCGAC 
CGTGTCCCCT ACGTGATCAT CGGTGCCGCC AAGGGCGTGG CTGCCTACAT GAAGTCCGAG 
GACCCCCCTC TTCGTGCTGG AGCACAGCCT GCCCATCGAC ACGCAGTACT GAGCAGCAGC 
TCGCCAAGCC ACTCCTGCGC ATCTTCGAGC CCATCCTGGG CGAGGGCCGC GCCGAGGCCG 
TGCTGCTGCG GGGGGACCAC ACGCGCTGCA AGACGGTGCT CACAGGCAAG GTGGGCGGCC 
TCCTGGCCTT CGCCCAGCGC CGCAGCTGCT GCATCGGCTG CCGCACGGTC CTCAGCCACC 
AGGGAGCCGT GTGCAAGTTC TGCCAGCCCC GGGAGTCGGA GCTCTACCAG AAGGAGGTGT 
CCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014685819.1
CDS	52..3180
Translation	MEGKRRPGPGPGVPPKRARGGLWDEDEPSGPSQFEEELALMEELEAERRLQEQEEEELQSALEGAAHGHLSQTAIDPRWLRPSPPPLDPQTEPLIFQQLEIDHYVGPAQPLPGAPTPSPGSVPVLRAFGVTDEGVSVCCHIHGFAPYFYTPAPPGFGPEHLSDLQRELNVAISRDQRGGKELAGPAVLAVELCSRESMFGYQGRGPSPFLRITLALPRLAAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYVLRPKGKTTLCQLEADVLWSDVISHAPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEELLQAWSAFIRTMDPDVITGYNIQNFDLPYLISRAQTLKVSSFPFLGRVSGLRSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMREGLVMPVVKTEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPGAAQKLGLTEDQFIKTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIERTKQLVESKYTTENGYSASAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPNGAVAHAQEVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIIGAAKGVAAYMKSEDPPLRAGAQPAHRHAVLSSSSPSHSCASSSPSWARAAPRPCCCGGTTRAARRCSQARWAASWPSPSAAAAASAAARSSATREPCASSASPGSRSSTRRRCPT

Target ORF information:

RefSeq Version	XM_014830333.1
Organism	Equus asinus(ass)
Definition	Equus asinus polymerase (DNA directed), delta 1, catalytic subunit (POLD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014830333.1

ORF Insert Sequence:

ATGGAGGGCA AGCGGCGGCC AGGCCCGGGG CCTGGGGTGC CCCCGAAGCG GGCCCGGGGG 
GGCCTCTGGG ATGAGGACGA GCCGTCGGGG CCGTCCCAGT TCGAGGAGGA GCTGGCGCTG 
ATGGAGGAGC TGGAGGCGGA GCGCAGGCTG CAGGAGCAGG AGGAGGAGGA GCTGCAGTCG 
GCCCTGGAGG GGGCTGCGCA CGGGCACCTC TCCCAGACAG CCATAGACCC CCGCTGGCTG 
CGGCCCTCCC CACCTCCCCT GGACCCCCAG ACGGAGCCCC TCATCTTCCA GCAGTTGGAG 
ATCGACCATT ATGTGGGCCC AGCGCAGCCC CTGCCTGGGG CACCCACGCC CTCCCCGGGC 
TCCGTGCCCG TGCTCCGTGC CTTCGGGGTC ACCGACGAGG GCGTCTCTGT CTGCTGCCAC 
ATCCACGGCT TCGCACCCTA CTTCTACACC CCGGCGCCCC CTGGTTTCGG GCCCGAGCAC 
CTGAGCGACC TGCAGCGGGA GCTGAATGTG GCCATCAGCC GGGACCAGCG CGGCGGGAAG 
GAGCTGGCGG GGCCAGCTGT GCTGGCTGTA GAGCTGTGCT CCCGGGAGAG CATGTTCGGG 
TACCAAGGGC GTGGCCCCTC CCCGTTTCTG CGCATCACCC TCGCGCTGCC CCGCCTTGCG 
GCCCCCGCCC GCCGCCTCCT GGAGCAGGGC ATCCGTGTGG CAGGCCTGGG CACCCCCAGC 
TTCGCGCCCT ATGAGGCCAA CGTCGACTTT GAGATCCGGT TCATGGTGGA CACGGACATC 
GTCGGCTGCA ACTGGCTGGA GCTCCCGGCC GGGAAATACG TCCTGAGGCC GAAGGGGAAG 
ACCACACTGT GTCAGCTGGA GGCAGACGTG CTGTGGTCGG ACGTGATCAG TCACGCACCA 
GAAGGGCAGT GGCAGCGAAT CGCCCCCCTG CGCGTGCTCA GCTTCGACAT CGAGTGCGCT 
GGCCGCAAAG GCATCTTCCC CGAACCCGAG CGGGACCCTG TCATCCAGAT CTGCTCGCTG 
GGCCTGCGCT GGGGCGAGCC GGAGCCCTTC CTGCGCCTGG CGCTCACCCT GCGGCCCTGT 
GCTCCCATCC TGGGCGCCAA GGTGCAGAGC TACGAGCGGG AGGAGGAGCT GCTGCAGGCC 
TGGTCGGCCT TCATCCGCAC CATGGACCCC GACGTGATCA CCGGCTACAA CATCCAGAAC 
TTCGACCTTC CCTACCTCAT CTCCCGGGCC CAGACCCTCA AGGTGTCGTC CTTCCCCTTC 
CTGGGCCGCG TGTCTGGCCT CCGCTCCAAC ATCCGGGACT CGTCGTTCCA GTCGAAGCAG 
ACCGGCCGGC GGGACACCAA GGTGGTCAGC ATGGTGGGCC GCGTACAGAT GGACATGCTG 
CAGGTGCTGC TGCGGGAGTA CAAGCTCCGC TCCTACACAC TCAACGCCGT GAGCTTCCAC 
TTCCTGGGCG AGCAGAAGGA GGACGTGCAG CACAGCATCA TCACCGACCT GCAGAACGGG 
AACGAGCAGA CGCGCCGCCG CCTGGCCGTG TACTGCCTCA AGGACGCCTT CCTGCCACTG 
CGGCTGCTCG AGCGGCTCAT GGTGCTGGTG AACGCCGTGG AGATGGCGCG TGTCACCGGC 
GTGCCCCTCA GCTACCTGCT CAGCCGTGGC CAGCAGGTCA AGGTCGTGTC CCAGCTGCTG 
CGACAGGCCA TGCGCGAGGG GCTGGTGATG CCCGTGGTGA AGACGGAGGG CGGTGAGGAC 
TACACGGGGG CCACAGTCAT CGAGCCCCTC AAAGGGTACT ACGATGTCCC CATCGCCACC 
CTGGACTTCT CCTCGCTGTA CCCGTCCATC ATGATGGCCC ACAACCTGTG CTACACCACG 
CTCCTGCGCC CCGGGGCCGC CCAGAAACTG GGCCTGACCG AGGATCAGTT CATCAAGACG 
CCCACAGGGG ACGAGTTTGT GAAGACGTCG GTGCGGAAGG GGCTCCTGCC TCAGATCCTG 
GAGAACCTGC TCAGCGCCCG GAAGAGGGCC AAGGCCGAGC TGGCCAAGGA GACAGACCCC 
TTGCGGCGGC AGGTCTTGGA CGGGCGGCAG CTGGCACTCA AAGTGAGCGC CAACTCTGTG 
TACGGCTTCA CAGGCGCCCA GGTGGGCAAG CTGCCATGCC TGGAGATCTC ACAGAGCGTC 
ACCGGGTTCG GGCGCCAGAT GATTGAAAGA ACAAAGCAGC TGGTGGAGTC CAAGTACACG 
ACGGAGAACG GCTACAGCGC GAGCGCCAAG GTGGTGTACG GTGACACCGA CTCCGTCATG 
TGCCGATTCG GCGTCTCCTC GGTGGCGGAG GCCATGGCCC TAGGGCGGGA GGCCGCCGAC 
TGGGTGTCGG GCCACTTCCC CTCGCCCATC CGGCTGGAGT TTGAGAAGGT CTACTTCCCC 
TACCTGCTCA TCAGCAAGAA GCGCTACGCC GGCCTGCTCT TCTCCTCCCG GCCGGATGCC 
CATGACCGCA TGGACTGCAA GGGCCTGGAG GCCGTGCGCA GGGACAACTG CCCCCTAGTG 
GCCAACCTTG TCACCGCCTC GCTGCGCCGC CTGCTCATCG ACCGAGACCC CAATGGCGCC 
GTGGCCCACG CACAGGAGGT CATCTCGGAC CTGCTGTGCA ACCGCATCGA CATCTCCCAG 
CTGGTCATCA CCAAGGAGCT GACCCGCGCG GCCGCCGACT ACGCCGGCAA ACAGGCCCAC 
GTAGAGCTGG CCGAGAGGAT GAGGAAGCGG GACCCCGGGA GCGCGCCCAG CCTGGGCGAC 
CGTGTCCCCT ACGTGATCAT CGGTGCCGCC AAGGGCGTGG CTGCCTACAT GAAGTCCGAG 
GACCCCCCTC TTCGTGCTGG AGCACAGCCT GCCCATCGAC ACGCAGTACT GAGCAGCAGC 
TCGCCAAGCC ACTCCTGCGC ATCTTCGAGC CCATCCTGGG CGAGGGCCGC GCCGAGGCCG 
TGCTGCTGCG GGGGGACCAC ACGCGCTGCA AGACGGTGCT CACAGGCAAG GTGGGCGGCC 
TCCTGGCCTT CGCCCAGCGC CGCAGCTGCT GCATCGGCTG CCGCACGGTC CTCAGCCACC 
AGGGAGCCGT GTGCAAGTTC TGCCAGCCCC GGGAGTCGGA GCTCTACCAG AAGGAGGTGT 
CCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLD1 ( XM_014830334.1 ) cDNA ORF clone, Equus asinus(ass) -> XP_014685820.1 Equus asinus polymerase (DNA directed), delta 1, catalytic subunit (POLD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEq30635
Clone ID Related Accession (Same CDS sequence)	XM_014830334.1
Accession Version	XM_014830334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-01
Organism	Equus asinus(ass)
Product	DNA polymerase delta catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014638557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus asinus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCA AGCGGCGGCC AGGCCCGGGG CCTGGGGTGC CCCCGAAGCG GGCCCGGGGG 
GGCCTCTGGG ATGAGGACGA GCCGTCGGGG CCGTCCCAGT TCGAGGAGGA GCTGGCGCTG 
ATGGAGGAGC TGGAGGCGGA GCGCAGGCTG CAGGAGCAGG AGGAGGAGGA GCTGCAGTCG 
GCCCTGGAGG GGGCTGCGCA CGGGCACCTC TCCCAGACAG CCATAGACCC CCGCTGGCTG 
CGGCCCTCCC CACCTCCCCT GGACCCCCAG ACGGAGCCCC TCATCTTCCA GCAGTTGGAG 
ATCGACCATT ATGTGGGCCC AGCGCAGCCC CTGCCTGGGG CACCCACGCC CTCCCCGGGC 
TCCGTGCCCG TGCTCCGTGC CTTCGGGGTC ACCGACGAGG GCGTCTCTGT CTGCTGCCAC 
ATCCACGGCT TCGCACCCTA CTTCTACACC CCGGCGCCCC CTGGTTTCGG GCCCGAGCAC 
CTGAGCGACC TGCAGCGGGA GCTGAATGTG GCCATCAGCC GGGACCAGCG CGGCGGGAAG 
GAGCTGGCGG GGCCAGCTGT GCTGGCTGTA GAGCTGTGCT CCCGGGAGAG CATGTTCGGG 
TACCAAGGGC GTGGCCCCTC CCCGTTTCTG CGCATCACCC TCGCGCTGCC CCGCCTTGCG 
GCCCCCGCCC GCCGCCTCCT GGAGCAGGGC ATCCGTGTGG CAGGCCTGGG CACCCCCAGC 
TTCGCGCCCT ATGAGGCCAA CGTCGACTTT GAGATCCGGT TCATGGTGGA CACGGACATC 
GTCGGCTGCA ACTGGCTGGA GCTCCCGGCC GGGAAATACG TCCTGAGGCC GAAGGGGAAG 
ACCACACTGT GTCAGCTGGA GGCAGACGTG CTGTGGTCGG ACGTGATCAG TCACGCACCA 
GAAGGGCAGT GGCAGCGAAT CGCCCCCCTG CGCGTGCTCA GCTTCGACAT CGAGTGCGCT 
GGCCGCAAAG GCATCTTCCC CGAACCCGAG CGGGACCCTG TCATCCAGAT CTGCTCGCTG 
GGCCTGCGCT GGGGCGAGCC GGAGCCCTTC CTGCGCCTGG CGCTCACCCT GCGGCCCTGT 
GCTCCCATCC TGGGCGCCAA GGTGCAGAGC TACGAGCGGG AGGAGGAGCT GCTGCAGGCC 
TGGTCGGCCT TCATCCGCAC CATGGACCCC GACGTGATCA CCGGCTACAA CATCCAGAAC 
TTCGACCTTC CCTACCTCAT CTCCCGGGCC CAGACCCTCA AGGTGTCGTC CTTCCCCTTC 
CTGGGCCGCG TGTCTGGCCT CCGCTCCAAC ATCCGGGACT CGTCGTTCCA GTCGAAGCAG 
ACCGGCCGGC GGGACACCAA GGTGGTCAGC ATGGTGGGCC GCGTACAGAT GGACATGCTG 
CAGGTGCTGC TGCGGGAGTA CAAGCTCCGC TCCTACACAC TCAACGCCGT GAGCTTCCAC 
TTCCTGGGCG AGCAGAAGGA GGACGTGCAG CACAGCATCA TCACCGACCT GCAGAACGGG 
AACGAGCAGA CGCGCCGCCG CCTGGCCGTG TACTGCCTCA AGGACGCCTT CCTGCCACTG 
CGGCTGCTCG AGCGGCTCAT GGTGCTGGTG AACGCCGTGG AGATGGCGCG TGTCACCGGC 
GTGCCCCTCA GCTACCTGCT CAGCCGTGGC CAGCAGGTCA AGGTCGTGTC CCAGCTGCTG 
CGACAGGCCA TGCGCGAGGG GCTGGTGATG CCCGTGGTGA AGACGGAGGG CGGTGAGGAC 
TACACGGGGG CCACAGTCAT CGAGCCCCTC AAAGGGTACT ACGATGTCCC CATCGCCACC 
CTGGACTTCT CCTCGCTGTA CCCGTCCATC ATGATGGCCC ACAACCTGTG CTACACCACG 
CTCCTGCGCC CCGGGGCCGC CCAGAAACTG GGCCTGACCG AGGATCAGTT CATCAAGACG 
CCCACAGGGG ACGAGTTTGT GAAGACGTCG GTGCGGAAGG GGCTCCTGCC TCAGATCCTG 
GAGAACCTGC TCAGCGCCCG GAAGAGGGCC AAGGCCGAGC TGGCCAAGGA GACAGACCCC 
TTGCGGCGGC AGGTCTTGGA CGGGCGGCAG CTGGCACTCA AAGTGAGCGC CAACTCTGTG 
TACGGCTTCA CAGGCGCCCA GGTGGGCAAG CTGCCATGCC TGGAGATCTC ACAGAGCGTC 
ACCGGGTTCG GGCGCCAGAT GATTGAAAGA ACAAAGCAGC TGGTGGAGTC CAAGTACACG 
ACGGAGAACG GCTACAGCGC GAGCGCCAAG GTGGTGTACG GTGACACCGA CTCCGTCATG 
TGCCGATTCG GCGTCTCCTC GGTGGCGGAG GCCATGGCCC TAGGGCGGGA GGCCGCCGAC 
TGGGTGTCGG GCCACTTCCC CTCGCCCATC CGGCTGGAGT TTGAGAAGGT CTACTTCCCC 
TACCTGCTCA TCAGCAAGAA GCGCTACGCC GGCCTGCTCT TCTCCTCCCG GCCGGATGCC 
CATGACCGCA TGGACTGCAA GGGCCTGGAG GCCGTGCGCA GGGACAACTG CCCCCTAGTG 
GCCAACCTTG TCACCGCCTC GCTGCGCCGC CTGCTCATCG ACCGAGACCC CAATGGCGCC 
GTGGCCCACG CACAGGAGGT CATCTCGGAC CTGCTGTGCA ACCGCATCGA CATCTCCCAG 
CTGGTCATCA CCAAGGAGCT GACCCGCGCG GCCGCCGACT ACGCCGGCAA ACAGGCCCAC 
GTAGAGCTGG CCGAGAGGAT GAGGAAGCGG GACCCCGGGA GCGCGCCCAG CCTGGGCGAC 
CGTGTCCCCT ACGTGATCAT CGGTGCCGCC AAGGGCGTGG CTGCCTACAT GAAGTCCGAG 
GTCAGGACCC CCCTCTTCGT GCTGGAGCAC AGCCTGCCCA TCGACACGCA GTACNNNNNN 
GAGCAGCAGC TCGCCAAGCC ACTCCTGCGC ATCTTCGAGC CCATCCTGGG CGAGGGCCGC 
GCCGAGGCCG TGCTGCTGCG GGGGGACCAC ACGCGCTGCA AGACGGTGCT CACAGGCAAG 
GTGGGCGGCC TCCTGGCCTT CGCCCAGCGC CGCAGCTGCT GCATCGGCTG CCGCACGGTC 
CTCAGCCACC AGGGAGCCGT GTGCAAGTTC TGCCAGCCCC GGGAGTCGGA GCTCTACCAG 
AAGGAGGTGT CCCACCTGAA CGCCCTGGAG GAGCGCTTCT CGCGCCTCTG GACCCAGTGC 
CAGCGCTGTC AGGGCAGCCT GCACGAGGAC GTCATCTGCA CCAGCCGGGA CTGCCCCATC 
TTCTACATGC GCAAGAAGGT GCGCAAGGAC CTGGAAGACC AGGAGCAGCT TTTGAGACGC 
TTCGGGCCCC CCGGCCCCGA GGCCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014685820.1
CDS	52..3381
Translation	MEGKRRPGPGPGVPPKRARGGLWDEDEPSGPSQFEEELALMEELEAERRLQEQEEEELQSALEGAAHGHLSQTAIDPRWLRPSPPPLDPQTEPLIFQQLEIDHYVGPAQPLPGAPTPSPGSVPVLRAFGVTDEGVSVCCHIHGFAPYFYTPAPPGFGPEHLSDLQRELNVAISRDQRGGKELAGPAVLAVELCSRESMFGYQGRGPSPFLRITLALPRLAAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYVLRPKGKTTLCQLEADVLWSDVISHAPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEELLQAWSAFIRTMDPDVITGYNIQNFDLPYLISRAQTLKVSSFPFLGRVSGLRSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMREGLVMPVVKTEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPGAAQKLGLTEDQFIKTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIERTKQLVESKYTTENGYSASAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPNGAVAHAQEVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIIGAAKGVAAYMKSEVRTPLFVLEHSLPIDTQYnullnullEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAQRRSCCIGCRTVLSHQGAVCKFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW

Target ORF information:

RefSeq Version	XM_014830334.1
Organism	Equus asinus(ass)
Definition	Equus asinus polymerase (DNA directed), delta 1, catalytic subunit (POLD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014830334.1

ORF Insert Sequence:

ATGGAGGGCA AGCGGCGGCC AGGCCCGGGG CCTGGGGTGC CCCCGAAGCG GGCCCGGGGG 
GGCCTCTGGG ATGAGGACGA GCCGTCGGGG CCGTCCCAGT TCGAGGAGGA GCTGGCGCTG 
ATGGAGGAGC TGGAGGCGGA GCGCAGGCTG CAGGAGCAGG AGGAGGAGGA GCTGCAGTCG 
GCCCTGGAGG GGGCTGCGCA CGGGCACCTC TCCCAGACAG CCATAGACCC CCGCTGGCTG 
CGGCCCTCCC CACCTCCCCT GGACCCCCAG ACGGAGCCCC TCATCTTCCA GCAGTTGGAG 
ATCGACCATT ATGTGGGCCC AGCGCAGCCC CTGCCTGGGG CACCCACGCC CTCCCCGGGC 
TCCGTGCCCG TGCTCCGTGC CTTCGGGGTC ACCGACGAGG GCGTCTCTGT CTGCTGCCAC 
ATCCACGGCT TCGCACCCTA CTTCTACACC CCGGCGCCCC CTGGTTTCGG GCCCGAGCAC 
CTGAGCGACC TGCAGCGGGA GCTGAATGTG GCCATCAGCC GGGACCAGCG CGGCGGGAAG 
GAGCTGGCGG GGCCAGCTGT GCTGGCTGTA GAGCTGTGCT CCCGGGAGAG CATGTTCGGG 
TACCAAGGGC GTGGCCCCTC CCCGTTTCTG CGCATCACCC TCGCGCTGCC CCGCCTTGCG 
GCCCCCGCCC GCCGCCTCCT GGAGCAGGGC ATCCGTGTGG CAGGCCTGGG CACCCCCAGC 
TTCGCGCCCT ATGAGGCCAA CGTCGACTTT GAGATCCGGT TCATGGTGGA CACGGACATC 
GTCGGCTGCA ACTGGCTGGA GCTCCCGGCC GGGAAATACG TCCTGAGGCC GAAGGGGAAG 
ACCACACTGT GTCAGCTGGA GGCAGACGTG CTGTGGTCGG ACGTGATCAG TCACGCACCA 
GAAGGGCAGT GGCAGCGAAT CGCCCCCCTG CGCGTGCTCA GCTTCGACAT CGAGTGCGCT 
GGCCGCAAAG GCATCTTCCC CGAACCCGAG CGGGACCCTG TCATCCAGAT CTGCTCGCTG 
GGCCTGCGCT GGGGCGAGCC GGAGCCCTTC CTGCGCCTGG CGCTCACCCT GCGGCCCTGT 
GCTCCCATCC TGGGCGCCAA GGTGCAGAGC TACGAGCGGG AGGAGGAGCT GCTGCAGGCC 
TGGTCGGCCT TCATCCGCAC CATGGACCCC GACGTGATCA CCGGCTACAA CATCCAGAAC 
TTCGACCTTC CCTACCTCAT CTCCCGGGCC CAGACCCTCA AGGTGTCGTC CTTCCCCTTC 
CTGGGCCGCG TGTCTGGCCT CCGCTCCAAC ATCCGGGACT CGTCGTTCCA GTCGAAGCAG 
ACCGGCCGGC GGGACACCAA GGTGGTCAGC ATGGTGGGCC GCGTACAGAT GGACATGCTG 
CAGGTGCTGC TGCGGGAGTA CAAGCTCCGC TCCTACACAC TCAACGCCGT GAGCTTCCAC 
TTCCTGGGCG AGCAGAAGGA GGACGTGCAG CACAGCATCA TCACCGACCT GCAGAACGGG 
AACGAGCAGA CGCGCCGCCG CCTGGCCGTG TACTGCCTCA AGGACGCCTT CCTGCCACTG 
CGGCTGCTCG AGCGGCTCAT GGTGCTGGTG AACGCCGTGG AGATGGCGCG TGTCACCGGC 
GTGCCCCTCA GCTACCTGCT CAGCCGTGGC CAGCAGGTCA AGGTCGTGTC CCAGCTGCTG 
CGACAGGCCA TGCGCGAGGG GCTGGTGATG CCCGTGGTGA AGACGGAGGG CGGTGAGGAC 
TACACGGGGG CCACAGTCAT CGAGCCCCTC AAAGGGTACT ACGATGTCCC CATCGCCACC 
CTGGACTTCT CCTCGCTGTA CCCGTCCATC ATGATGGCCC ACAACCTGTG CTACACCACG 
CTCCTGCGCC CCGGGGCCGC CCAGAAACTG GGCCTGACCG AGGATCAGTT CATCAAGACG 
CCCACAGGGG ACGAGTTTGT GAAGACGTCG GTGCGGAAGG GGCTCCTGCC TCAGATCCTG 
GAGAACCTGC TCAGCGCCCG GAAGAGGGCC AAGGCCGAGC TGGCCAAGGA GACAGACCCC 
TTGCGGCGGC AGGTCTTGGA CGGGCGGCAG CTGGCACTCA AAGTGAGCGC CAACTCTGTG 
TACGGCTTCA CAGGCGCCCA GGTGGGCAAG CTGCCATGCC TGGAGATCTC ACAGAGCGTC 
ACCGGGTTCG GGCGCCAGAT GATTGAAAGA ACAAAGCAGC TGGTGGAGTC CAAGTACACG 
ACGGAGAACG GCTACAGCGC GAGCGCCAAG GTGGTGTACG GTGACACCGA CTCCGTCATG 
TGCCGATTCG GCGTCTCCTC GGTGGCGGAG GCCATGGCCC TAGGGCGGGA GGCCGCCGAC 
TGGGTGTCGG GCCACTTCCC CTCGCCCATC CGGCTGGAGT TTGAGAAGGT CTACTTCCCC 
TACCTGCTCA TCAGCAAGAA GCGCTACGCC GGCCTGCTCT TCTCCTCCCG GCCGGATGCC 
CATGACCGCA TGGACTGCAA GGGCCTGGAG GCCGTGCGCA GGGACAACTG CCCCCTAGTG 
GCCAACCTTG TCACCGCCTC GCTGCGCCGC CTGCTCATCG ACCGAGACCC CAATGGCGCC 
GTGGCCCACG CACAGGAGGT CATCTCGGAC CTGCTGTGCA ACCGCATCGA CATCTCCCAG 
CTGGTCATCA CCAAGGAGCT GACCCGCGCG GCCGCCGACT ACGCCGGCAA ACAGGCCCAC 
GTAGAGCTGG CCGAGAGGAT GAGGAAGCGG GACCCCGGGA GCGCGCCCAG CCTGGGCGAC 
CGTGTCCCCT ACGTGATCAT CGGTGCCGCC AAGGGCGTGG CTGCCTACAT GAAGTCCGAG 
GTCAGGACCC CCCTCTTCGT GCTGGAGCAC AGCCTGCCCA TCGACACGCA GTACNNNNNN 
GAGCAGCAGC TCGCCAAGCC ACTCCTGCGC ATCTTCGAGC CCATCCTGGG CGAGGGCCGC 
GCCGAGGCCG TGCTGCTGCG GGGGGACCAC ACGCGCTGCA AGACGGTGCT CACAGGCAAG 
GTGGGCGGCC TCCTGGCCTT CGCCCAGCGC CGCAGCTGCT GCATCGGCTG CCGCACGGTC 
CTCAGCCACC AGGGAGCCGT GTGCAAGTTC TGCCAGCCCC GGGAGTCGGA GCTCTACCAG 
AAGGAGGTGT CCCACCTGAA CGCCCTGGAG GAGCGCTTCT CGCGCCTCTG GACCCAGTGC 
CAGCGCTGTC AGGGCAGCCT GCACGAGGAC GTCATCTGCA CCAGCCGGGA CTGCCCCATC 
TTCTACATGC GCAAGAAGGT GCGCAAGGAC CTGGAAGACC AGGAGCAGCT TTTGAGACGC 
TTCGGGCCCC CCGGCCCCGA GGCCTGGTGA