NEFM cDNA ORF clone, Egretta garzetta(little egret)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFM
Entrez Gene ID	104123542
Full Name	neurofilament medium
Gene Type	protein-coding
Organism	Egretta garzetta(little egret)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009635805.1	XP_009634100.1	neurofilament medium polypeptide

Pathways from BioSystems
Gene Ontology

The following NEFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEg04802	XM_009635805.1 Latest version!	Egretta garzetta neurofilament, medium polypeptide (NEFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFM ( XM_009635805.1 ) cDNA ORF clone, Egretta garzetta(little egret) -> XP_009634100.1 Egretta garzetta neurofilament, medium polypeptide (NEFM), mRNA.

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CloneID	OEg04802
Clone ID Related Accession (Same CDS sequence)	XM_009635805.1
Accession Version	XM_009635805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-15
Organism	Egretta garzetta(little egret)
Product	neurofilament medium polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009259194.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Egretta garzetta Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGACCAGCG ACAGCACCTC AAATGCTTAT GGACTGAAGA AACTAGATGG ACACAAGAGG 
AGAAAACAAG AGACTACACT GCACGACAGG AAGCACGCCG GGCGGGCTCA GCTGAGCGAC 
GCCTACGAGC AGGAGCTGCG GGAGCTGCGG GGAGGCCTGG AGCAGGTGAG CCACGAGAAG 
GCGCAGATCC AGCTGGACTC GGAGCACATC GAGGAGGACA TCCAGCGCCT GCGGGAGCGC 
TTCGAGGATG AGGCGCGGCT GCGCGACGAG ACGGAGGCCA CCATCCGCGC CCTGCGCAAG 
GAGATGGAGG AGGCCTCGCT GATGCGGGCG GAGCTGGACA AGAAGGTGCA GTCGCTGCAG 
GACGAGGTGG CTTTCCTGCG GGGCAACCAT GAGGAGGAGG TGGCCGAGCT GCTGGCGCAG 
CTCCAGGCGT CCCATGCCAC GGTGGAGAGG AAGGACTACC TGAAGACCGA CCTCACCACG 
GCGCTGAAGG AGATCCGCGC CCAGCTGGAG TGCCAGTCCG ACCACAACAT GCACCAGGCT 
GAGGAGTGGT TCAAGTGCCG CTACGCCAAG CTGACCGAGG CGGCCGAGCA GAACAAGGAG 
GCCATCCGCT CCGCCAAGGA GGAGATCGCC GAGTACCGCC GGCAGCTGCA GTCCAAGAGC 
ATCGAGCTGG AGTCGGTGCG TGGCACCAAG GAGTCGCTGG AGCGGCAGCT CAGTGACATT 
GAGGAACGCC ACAACAACGA CCTCACCACC TACCAGGACA CGATTCATCA GCTGGAGAAC 
GAGCTTAGAG GAACAAAGTG GGAAATGGCA CGTCACTTGA GGGAATACCA GGACCTCCTC 
AATGTCAAGA TGGCCCTGGA TATTGAAATT GCTGCATACA GGAAGCTACT GGAGGGCGAA 
GAGACAAGAT TCAGTGCCTT CTCTGGAAGC ATTACCGGAC CCATATTCAC ACACAGACAA 
CCCTCTGTCA CAATAGCGTC CACTAAAATT CAGAAAACAA AAATTGAACC ACCAAAGCTG 
AAGGTCCAGC ACAAGTTTGT AGAAGAAATC ATTGAAGAGA CAAAGGTAGA GGATGAAAAG 
TCTGAAATGG AAGATGCCCT AGCAGCTATT GCAGAGGAAA TGGCAGCCAA GGCCCAGCAA 
GAAGAACAGG AGGAAGAAAA AGCAGAAGAA GCAGCTGCAG AGGAAGAAAT TGTTTCTGAG 
AAGGCTGCTG CAGAACAAGC AGCTGCACCT GAGGAAGAAG AGAAGGAGGA AGAGGAAGCA 
GAGGAGGAAG AAGAAGCTGC AAAATCTGAC GCAGCGGAAG AAGGAGGTTC TGAAAAAGAA 
GAAATAGAGG AAAAGGAAGA AGGGGAGGAG GCTGAGGAAG AGGGGGAAGA AGCTGAGGCC 
AAGGGCAAAG CTGAAGAGGT AGCAGCGAAG GTAGAGAAGG TCAAAACACC TCCCTCAAAG 
TCACCCCCCA AATCCCCCCC CAAATCCCCT GTAACGGAGC CGGCCAAGGC TGTCCAGAAA 
GAAACACCTG CAGAAGCAGG AAAAGAACAG AAGGTGGAGA AAGGTGTTGA GAAACCAGCC 
AAGGAGGAGG AGAAAGCAGC ATCTCCGGAG AAGCCAGCGA CACCAAAGGT AACCTCCCCG 
GAGAAGCCTG CAACCCCAGA GAAGCCTGCG ACGCCGGAGA AGCCTGCAAC CCCAGAGAAA 
GCAGTGACCC TGGAGAAGCT GGCCACCCCA GAAAAGCCCC GTTCTCCAGA AAAGCCAGCG 
ACCCCAGAAA AGCCCCGTTC TCCAGAAAAG CCAGTGACTC CGGAGAAGCC CCGTTCTCCA 
GAGAAGCCAG CCTCCCCGGT CAAAGATGAA AAGGCTGTGG TGGAGGAGAC CATCACTGTC 
ACAAAGGTAA CAAAAATTAG TGCAGAGGTA GAGAAGGAGT CCAGGAAAGA AGACATTGCA 
GTGAATGGTG AGGTGGAGGA GAAAAAGGGA GCAGAGGAAT CCAAAGAGAA GGAGGCTGAG 
GAGGAAGACA AGGGAGTTGT CACCAATGGC CTAGATGTGA GCCCAATTGA TGATAAGGGT 
GAGAAAATTG TAGTAACCAA AAAAGCAGAG AAAATCACTG AAGGTGGGGA CAGTACAACC 
ACATATATCA CCAAGTCAGT GACAGTCACT CAGAAGGTAG AGGAACATGA AGAAAGCTTT 
GAGGAGAAAT TAGTGTCCAC CAAGAAAGTG GAGAAAGTTA CTTCACATGC TGTAGTAAAA 
GAGATTAAGG AGACCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009634100.1
CDS	1..2241
Translation	MTSDSTSNAYGLKKLDGHKRRKQETTLHDRKHAGRAQLSDAYEQELRELRGGLEQVSHEKAQIQLDSEHIEEDIQRLRERFEDEARLRDETEATIRALRKEMEEASLMRAELDKKVQSLQDEVAFLRGNHEEEVAELLAQLQASHATVERKDYLKTDLTTALKEIRAQLECQSDHNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNNDLTTYQDTIHQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSAFSGSITGPIFTHRQPSVTIASTKIQKTKIEPPKLKVQHKFVEEIIEETKVEDEKSEMEDALAAIAEEMAAKAQQEEQEEEKAEEAAAEEEIVSEKAAAEQAAAPEEEEKEEEEAEEEEEAAKSDAAEEGGSEKEEIEEKEEGEEAEEEGEEAEAKGKAEEVAAKVEKVKTPPSKSPPKSPPKSPVTEPAKAVQKETPAEAGKEQKVEKGVEKPAKEEEKAASPEKPATPKVTSPEKPATPEKPATPEKPATPEKAVTLEKLATPEKPRSPEKPATPEKPRSPEKPVTPEKPRSPEKPASPVKDEKAVVEETITVTKVTKISAEVEKESRKEDIAVNGEVEEKKGAEESKEKEAEEEDKGVVTNGLDVSPIDDKGEKIVVTKKAEKITEGGDSTTTYITKSVTVTQKVEEHEESFEEKLVSTKKVEKVTSHAVVKEIKETE

Target ORF information:

RefSeq Version	XM_009635805.1
Organism	Egretta garzetta(little egret)
Definition	Egretta garzetta neurofilament, medium polypeptide (NEFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009635805.1

ORF Insert Sequence:

ATGACCAGCG ACAGCACCTC AAATGCTTAT GGACTGAAGA AACTAGATGG ACACAAGAGG 
AGAAAACAAG AGACTACACT GCACGACAGG AAGCACGCCG GGCGGGCTCA GCTGAGCGAC 
GCCTACGAGC AGGAGCTGCG GGAGCTGCGG GGAGGCCTGG AGCAGGTGAG CCACGAGAAG 
GCGCAGATCC AGCTGGACTC GGAGCACATC GAGGAGGACA TCCAGCGCCT GCGGGAGCGC 
TTCGAGGATG AGGCGCGGCT GCGCGACGAG ACGGAGGCCA CCATCCGCGC CCTGCGCAAG 
GAGATGGAGG AGGCCTCGCT GATGCGGGCG GAGCTGGACA AGAAGGTGCA GTCGCTGCAG 
GACGAGGTGG CTTTCCTGCG GGGCAACCAT GAGGAGGAGG TGGCCGAGCT GCTGGCGCAG 
CTCCAGGCGT CCCATGCCAC GGTGGAGAGG AAGGACTACC TGAAGACCGA CCTCACCACG 
GCGCTGAAGG AGATCCGCGC CCAGCTGGAG TGCCAGTCCG ACCACAACAT GCACCAGGCT 
GAGGAGTGGT TCAAGTGCCG CTACGCCAAG CTGACCGAGG CGGCCGAGCA GAACAAGGAG 
GCCATCCGCT CCGCCAAGGA GGAGATCGCC GAGTACCGCC GGCAGCTGCA GTCCAAGAGC 
ATCGAGCTGG AGTCGGTGCG TGGCACCAAG GAGTCGCTGG AGCGGCAGCT CAGTGACATT 
GAGGAACGCC ACAACAACGA CCTCACCACC TACCAGGACA CGATTCATCA GCTGGAGAAC 
GAGCTTAGAG GAACAAAGTG GGAAATGGCA CGTCACTTGA GGGAATACCA GGACCTCCTC 
AATGTCAAGA TGGCCCTGGA TATTGAAATT GCTGCATACA GGAAGCTACT GGAGGGCGAA 
GAGACAAGAT TCAGTGCCTT CTCTGGAAGC ATTACCGGAC CCATATTCAC ACACAGACAA 
CCCTCTGTCA CAATAGCGTC CACTAAAATT CAGAAAACAA AAATTGAACC ACCAAAGCTG 
AAGGTCCAGC ACAAGTTTGT AGAAGAAATC ATTGAAGAGA CAAAGGTAGA GGATGAAAAG 
TCTGAAATGG AAGATGCCCT AGCAGCTATT GCAGAGGAAA TGGCAGCCAA GGCCCAGCAA 
GAAGAACAGG AGGAAGAAAA AGCAGAAGAA GCAGCTGCAG AGGAAGAAAT TGTTTCTGAG 
AAGGCTGCTG CAGAACAAGC AGCTGCACCT GAGGAAGAAG AGAAGGAGGA AGAGGAAGCA 
GAGGAGGAAG AAGAAGCTGC AAAATCTGAC GCAGCGGAAG AAGGAGGTTC TGAAAAAGAA 
GAAATAGAGG AAAAGGAAGA AGGGGAGGAG GCTGAGGAAG AGGGGGAAGA AGCTGAGGCC 
AAGGGCAAAG CTGAAGAGGT AGCAGCGAAG GTAGAGAAGG TCAAAACACC TCCCTCAAAG 
TCACCCCCCA AATCCCCCCC CAAATCCCCT GTAACGGAGC CGGCCAAGGC TGTCCAGAAA 
GAAACACCTG CAGAAGCAGG AAAAGAACAG AAGGTGGAGA AAGGTGTTGA GAAACCAGCC 
AAGGAGGAGG AGAAAGCAGC ATCTCCGGAG AAGCCAGCGA CACCAAAGGT AACCTCCCCG 
GAGAAGCCTG CAACCCCAGA GAAGCCTGCG ACGCCGGAGA AGCCTGCAAC CCCAGAGAAA 
GCAGTGACCC TGGAGAAGCT GGCCACCCCA GAAAAGCCCC GTTCTCCAGA AAAGCCAGCG 
ACCCCAGAAA AGCCCCGTTC TCCAGAAAAG CCAGTGACTC CGGAGAAGCC CCGTTCTCCA 
GAGAAGCCAG CCTCCCCGGT CAAAGATGAA AAGGCTGTGG TGGAGGAGAC CATCACTGTC 
ACAAAGGTAA CAAAAATTAG TGCAGAGGTA GAGAAGGAGT CCAGGAAAGA AGACATTGCA 
GTGAATGGTG AGGTGGAGGA GAAAAAGGGA GCAGAGGAAT CCAAAGAGAA GGAGGCTGAG 
GAGGAAGACA AGGGAGTTGT CACCAATGGC CTAGATGTGA GCCCAATTGA TGATAAGGGT 
GAGAAAATTG TAGTAACCAA AAAAGCAGAG AAAATCACTG AAGGTGGGGA CAGTACAACC 
ACATATATCA CCAAGTCAGT GACAGTCACT CAGAAGGTAG AGGAACATGA AGAAAGCTTT 
GAGGAGAAAT TAGTGTCCAC CAAGAAAGTG GAGAAAGTTA CTTCACATGC TGTAGTAAAA 
GAGATTAAGG AGACCGAATA A