EDEM3 cDNA ORF clone, Echinops telfairi(small Madagascar hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	EDEM3
Entrez Gene ID	101657839
Full Name	ER degradation enhancing alpha-mannosidase like protein 3
Gene Type	protein-coding
Organism	Echinops telfairi(small Madagascar hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004706676.1	XP_004706733.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
XM_004706675.1	XP_004706732.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1

Pathways from BioSystems
Gene Ontology

The following EDEM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDEM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEc13695	XM_004706675.1 Latest version!	Echinops telfairi ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEc13696	XM_004706676.1 Latest version!	Echinops telfairi ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDEM3 ( XM_004706675.1 ) cDNA ORF clone, Echinops telfairi(small Madagascar hedgehog) -> XP_004706732.1 Echinops telfairi ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEc13695
Clone ID Related Accession (Same CDS sequence)	XM_004706675.1
Accession Version	XM_004706675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-18
Organism	Echinops telfairi(small Madagascar hedgehog)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022111324.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Echinops telfairi Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAAG CCGGCGGCGG CCCGGGCTGG GGGTTCCCGG TCCCCCAGCG AGCGCCATGG 
AGCCTGGTGG CGGCGACGGC CGCGCTCTGC CTGGTGTCAG CCACGTCCGT GTGGCCGGCG 
GGGGCCGCGC CCATGAGTCG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG 
TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCC 
TTAACCTGCA GAGGTCGAGT TCGAGGCCAG GAGCCAAGTC GCGGTGATGT TGATGATGCC 
TTGGGAAAAT TTTCTCTGAC ACTGATTGAT TCTTTGGACA CTCTCGTGGT ACTAAATAAA 
ACTAAAGAAT TTGAAGATGC TGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT 
GTAGTTGTAT CCGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCTTTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT 
CTCCAAATGG CAAAGCAATT GGGTTACAAA CTTCTACCAG CTTTCAATAC TACCAGTGGC 
CTTCCTTATC CAAGAATTAA TTTAAAGTTT GGGATCCGAA AACCAGAAGC TCGAACAGGA 
ACTGAGACAG ATACCTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT TGCTGCTTTA 
AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GGAAAGCTCT TGATTTTCTC 
TGGGAAAAAA GACAACGGAG TAGTAACTTA GTGGGTGTGA CTATCAATAT TCACACTGGA 
GACTGGGTCA GAAAAGATAG TGGAGTTGGA GCAGGAATTG ATTCTTATTA TGAATATCTG 
TTGAAAGCCT ATGTCTTGCT TGGGGATGAC AGTTTTCTGG AAAGATTTAA TACACACTAT 
GACGCCATAA TGAGGTACAT TAGCCAGCCT CCTCTACTAC TTGACGTGCA TATCCACAAA 
CCAATGCTGA ACGCTCGGAC GTGGATGGAT GCTCTCCTTG CCTTTTTCCC AGGCTTACAG 
GTTTTAAAGG GAGATATTAG ACCTGCAATT GAAACACATG AAATGTTATA TCAAGTGATT 
AAAAAACATA ATTTTCTACC AGAGGCATTT ACAATAGATT TCAGGGTACA CTGGGCTCAA 
CACCCTTTAA GGCCTGAGTT TGCAGAAAGC ACCTACTTCT TATATAAAGC TACCGGGGAT 
CCTTACTACC TTGAAGTAGG AAAAACACTT ATTGAAAATT TAAACAAATA TGCTAGAGTG 
CCTTGTGGAT TCGCTGCCAT GAAGGATGTT CGTACAGGAA GTCATGAGGA CAGAATGGAT 
TCTTTCTTTT TGGCTGAAAT GTTTAAATAC CTTTACCTAT TATTTGCGGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA TTACATTTTT ACCACAGAGG CTCATCTGTT ACCTCTGTGG 
CTCTCCACCA CAAATCAGAG CATCCCCAAG AAGAACACGA CTTCAGAATA TACAGAACTG 
GATGACAGTA ATTTTGATTG GACTTGTCCA AATACTCAGA TTCTCTTCCC TAATGACCCA 
TTGTATGCTC AGAGCATCCG TGAGCCCTTA AAAAATGTGG TGGACAAGAG CTGTCCTAGA 
GGCATCATTA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCTCCTCT GAGAGCCCGA 
GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCCTGA AGAAGATGGG GGTGAGTTTG 
ATTCACCTCA AAGACGGGAG AGTTCAGTTG GTCCAACATG CAATCCAAGC TGCAAGTTCC 
ATTGATGCTG AAGATGGGTT GAGGTTCATG CAGGAGATGA TTGAACTGTC AAGTCAGCAG 
CAGAAAGAAC AGCAGCTCCC CCCACGGGCT GTGCAGATTG TTTCCCACCC GTTTTTTGGC 
AGGGTCGTGT TGACCGCTGG GCCAGCTCAG TTTGGGCTGG ATCTGTCGAA GCATAAAGAG 
ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GCTCAGAACT TACCAACCCA 
GAAGCAGTGA TGGGGAAAAT TGCTCTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG 
GCGCGCAACA TCCAGAACGC TGGCGCCATT GGCGGCATTG TTATTGACGA CAATGAGGGG 
AGCAGCAGCG ATACCGCCCC TCTGTTCCAG ATGGCTGGGG ATGGGAAGGA CACAGACGAC 
ATCAAGATCC CCATGCTCTT CCTATTCAGC AAAGAAGGAA GCATCATCCT GGATGCTATT 
CGGGAATATG AGGACGTAGA AGTGCTCCTT TCTGATAAAG CAAAAGATCG AGCAGCAATC 
TTAAAAGGTA AAATGATTCC AAACTACATT ATTAATAGTA ATCCTGAGAT GGAAAGTGAA 
GAACAGCCAT CCTCTGAAAA TGATCCTCCA AATCAGAGTG CCGAACAGAT CATCACATCA 
AGTTCTCAGG ACCCTGATCT GGCTGAGCAA GCGGCTCCTG AAGAACGTGC TCTAAGCCCT 
CCCCCGGAAC CAGCGGGCGC ACCATCTCCA GCAGAGATGG ACAATGCTGC AAACATTTCC 
CCCTCCGAAC AGAATTCCGA CCCCACAGAA AACCACGAGA GCACAAGTCT CGGTGGTGAA 
TGTACAGATT TAGACGACCA ACCTCAAGAG CCATCAGAAA CGGAGGAAGA CTCCAATCCT 
AATGTTAGCT GGGGCCAAAA AGCCCAGCCT ATAGACTCCA TATTAGCAGA CTGGAATGAA 
GATATAGAAG CTTTTGAAAT GATGGAGAAA GATGAACTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004706732.1
CDS	1..2862
Translation	MSEAGGGPGWGFPVPQRAPWSLVAATAALCLVSATSVWPAGAAPMSREERQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTIDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNQSIPKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSIDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPYNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREYEDVEVLLSDKAKDRAAILKGKMIPNYIINSNPEMESEEQPSSENDPPNQSAEQIITSSSQDPDLAEQAAPEERALSPPPEPAGAPSPAEMDNAANISPSEQNSDPTENHESTSLGGECTDLDDQPQEPSETEEDSNPNVSWGQKAQPIDSILADWNEDIEAFEMMEKDEL

Target ORF information:

RefSeq Version	XM_004706675.1
Organism	Echinops telfairi(small Madagascar hedgehog)
Definition	Echinops telfairi ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004706675.1

ORF Insert Sequence:

ATGAGCGAAG CCGGCGGCGG CCCGGGCTGG GGGTTCCCGG TCCCCCAGCG AGCGCCATGG 
AGCCTGGTGG CGGCGACGGC CGCGCTCTGC CTGGTGTCAG CCACGTCCGT GTGGCCGGCG 
GGGGCCGCGC CCATGAGTCG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG 
TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCC 
TTAACCTGCA GAGGTCGAGT TCGAGGCCAG GAGCCAAGTC GCGGTGATGT TGATGATGCC 
TTGGGAAAAT TTTCTCTGAC ACTGATTGAT TCTTTGGACA CTCTCGTGGT ACTAAATAAA 
ACTAAAGAAT TTGAAGATGC TGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT 
GTAGTTGTAT CCGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCTTTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT 
CTCCAAATGG CAAAGCAATT GGGTTACAAA CTTCTACCAG CTTTCAATAC TACCAGTGGC 
CTTCCTTATC CAAGAATTAA TTTAAAGTTT GGGATCCGAA AACCAGAAGC TCGAACAGGA 
ACTGAGACAG ATACCTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT TGCTGCTTTA 
AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GGAAAGCTCT TGATTTTCTC 
TGGGAAAAAA GACAACGGAG TAGTAACTTA GTGGGTGTGA CTATCAATAT TCACACTGGA 
GACTGGGTCA GAAAAGATAG TGGAGTTGGA GCAGGAATTG ATTCTTATTA TGAATATCTG 
TTGAAAGCCT ATGTCTTGCT TGGGGATGAC AGTTTTCTGG AAAGATTTAA TACACACTAT 
GACGCCATAA TGAGGTACAT TAGCCAGCCT CCTCTACTAC TTGACGTGCA TATCCACAAA 
CCAATGCTGA ACGCTCGGAC GTGGATGGAT GCTCTCCTTG CCTTTTTCCC AGGCTTACAG 
GTTTTAAAGG GAGATATTAG ACCTGCAATT GAAACACATG AAATGTTATA TCAAGTGATT 
AAAAAACATA ATTTTCTACC AGAGGCATTT ACAATAGATT TCAGGGTACA CTGGGCTCAA 
CACCCTTTAA GGCCTGAGTT TGCAGAAAGC ACCTACTTCT TATATAAAGC TACCGGGGAT 
CCTTACTACC TTGAAGTAGG AAAAACACTT ATTGAAAATT TAAACAAATA TGCTAGAGTG 
CCTTGTGGAT TCGCTGCCAT GAAGGATGTT CGTACAGGAA GTCATGAGGA CAGAATGGAT 
TCTTTCTTTT TGGCTGAAAT GTTTAAATAC CTTTACCTAT TATTTGCGGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA TTACATTTTT ACCACAGAGG CTCATCTGTT ACCTCTGTGG 
CTCTCCACCA CAAATCAGAG CATCCCCAAG AAGAACACGA CTTCAGAATA TACAGAACTG 
GATGACAGTA ATTTTGATTG GACTTGTCCA AATACTCAGA TTCTCTTCCC TAATGACCCA 
TTGTATGCTC AGAGCATCCG TGAGCCCTTA AAAAATGTGG TGGACAAGAG CTGTCCTAGA 
GGCATCATTA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCTCCTCT GAGAGCCCGA 
GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCCTGA AGAAGATGGG GGTGAGTTTG 
ATTCACCTCA AAGACGGGAG AGTTCAGTTG GTCCAACATG CAATCCAAGC TGCAAGTTCC 
ATTGATGCTG AAGATGGGTT GAGGTTCATG CAGGAGATGA TTGAACTGTC AAGTCAGCAG 
CAGAAAGAAC AGCAGCTCCC CCCACGGGCT GTGCAGATTG TTTCCCACCC GTTTTTTGGC 
AGGGTCGTGT TGACCGCTGG GCCAGCTCAG TTTGGGCTGG ATCTGTCGAA GCATAAAGAG 
ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GCTCAGAACT TACCAACCCA 
GAAGCAGTGA TGGGGAAAAT TGCTCTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG 
GCGCGCAACA TCCAGAACGC TGGCGCCATT GGCGGCATTG TTATTGACGA CAATGAGGGG 
AGCAGCAGCG ATACCGCCCC TCTGTTCCAG ATGGCTGGGG ATGGGAAGGA CACAGACGAC 
ATCAAGATCC CCATGCTCTT CCTATTCAGC AAAGAAGGAA GCATCATCCT GGATGCTATT 
CGGGAATATG AGGACGTAGA AGTGCTCCTT TCTGATAAAG CAAAAGATCG AGCAGCAATC 
TTAAAAGGTA AAATGATTCC AAACTACATT ATTAATAGTA ATCCTGAGAT GGAAAGTGAA 
GAACAGCCAT CCTCTGAAAA TGATCCTCCA AATCAGAGTG CCGAACAGAT CATCACATCA 
AGTTCTCAGG ACCCTGATCT GGCTGAGCAA GCGGCTCCTG AAGAACGTGC TCTAAGCCCT 
CCCCCGGAAC CAGCGGGCGC ACCATCTCCA GCAGAGATGG ACAATGCTGC AAACATTTCC 
CCCTCCGAAC AGAATTCCGA CCCCACAGAA AACCACGAGA GCACAAGTCT CGGTGGTGAA 
TGTACAGATT TAGACGACCA ACCTCAAGAG CCATCAGAAA CGGAGGAAGA CTCCAATCCT 
AATGTTAGCT GGGGCCAAAA AGCCCAGCCT ATAGACTCCA TATTAGCAGA CTGGAATGAA 
GATATAGAAG CTTTTGAAAT GATGGAGAAA GATGAACTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDEM3 ( XM_004706676.1 ) cDNA ORF clone, Echinops telfairi(small Madagascar hedgehog) -> XP_004706733.1 Echinops telfairi ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEc13696
Clone ID Related Accession (Same CDS sequence)	XM_004706676.1
Accession Version	XM_004706676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-18
Organism	Echinops telfairi(small Madagascar hedgehog)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022111324.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Echinops telfairi Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAAG CCGGCGGCGG CCCGGGCTGG GGGTTCCCGG TCCCCCAGCG AGCGCCATGG 
AGCCTGGTGG CGGCGACGGC CGCGCTCTGC CTGGTGTCAG CCACGTCCGT GTGGCCGGCG 
GGGGCCGCGC CCATGAGTCG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG 
TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCC 
TTAACCTGCA GAGGTCGAGT TCGAGGCCAG GAGCCAAGTC GCGGTGATGT TGATGATGCC 
TTGGGAAAAT TTTCTCTGAC ACTGATTGAT TCTTTGGACA CTCTCGTGGT ACTAAATAAA 
ACTAAAGAAT TTGAAGATGC TGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT 
GTAGTTGTAT CCGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCTTTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT 
CTCCAAATGG CAAAGCAATT GGGTTACAAA CTTCTACCAG CTTTCAATAC TACCAGTGGC 
CTTCCTTATC CAAGAATTAA TTTAAAGTTT GGGATCCGAA AACCAGAAGC TCGAACAGGA 
ACTGAGACAG ATACCTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT TGCTGCTTTA 
AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GGAAAGCTCT TGATTTTCTC 
TGGGAAAAAA GACAACGGAG TAGTAACTTA GTGGGTGTGA CTATCAATAT TCACACTGGA 
GACTGGGTCA GAAAAGATAG TGGAGTTGGA GCAGGAATTG ATTCTTATTA TGAATATCTG 
TTGAAAGCCT ATGTCTTGCT TGGGGATGAC AGTTTTCTGG AAAGATTTAA TACACACTAT 
GACGCCATAA TGAGGTACAT TAGCCAGCCT CCTCTACTAC TTGACGTGCA TATCCACAAA 
CCAATGCTGA ACGCTCGGAC GTGGATGGAT GCTCTCCTTG CCTTTTTCCC AGGCTTACAG 
GTTTTAAAGG GAGATATTAG ACCTGCAATT GAAACACATG AAATGTTATA TCAAGTGATT 
AAAAAACATA ATTTTCTACC AGAGGCATTT ACAATAGATT TCAGGGTACA CTGGGCTCAA 
CACCCTTTAA GGCCTGAGTT TGCAGAAAGC ACCTACTTCT TATATAAAGC TACCGGGGAT 
CCTTACTACC TTGAAGTAGG AAAAACACTT ATTGAAAATT TAAACAAATA TGCTAGAGTG 
CCTTGTGGAT TCGCTGCCAT GAAGGATGTT CGTACAGGAA GTCATGAGGA CAGAATGGAT 
TCTTTCTTTT TGGCTGAAAT GTTTAAATAC CTTTACCTAT TATTTGCGGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA TTACATTTTT ACCACAGAGG CTCATCTGTT ACCTCTGTGG 
CTCTCCACCA CAAATCAGAG CATCCCCAAG AAGAACACGA CTTCAGAATA TACAGAACTG 
GATGACAGTA ATTTTGATTG GACTTGTCCA AATACTCAGA TTCTCTTCCC TAATGACCCA 
TTGTATGCTC AGAGCATCCG TGAGCCCTTA AAAAATGTGG TGGACAAGAG CTGTCCTAGA 
GGCATCATTA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCTCCTCT GAGAGCCCGA 
GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCCTGA AGAAGATGGG GGTGAGTTTG 
ATTCACCTCA AAGACGGGAG AGTTCAGTTG GTCCAACATG CAATCCAAGC TGCAAGTTCC 
ATTGATGCTG AAGATGGGTT GAGGTTCATG CAGGAGATGA TTGAACTGTC AAGTCAGCAG 
CAGAAAGAAC AGCAGCTCCC CCCACGGGCT GTGCAGATTG TTTCCCACCC GTTTTTTGGC 
AGGGTCGTGT TGACCGCTGG GCCAGCTCAG TTTGGGCTGG ATCTGTCGAA GCATAAAGAG 
ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GCTCAGAACT TACCAACCCA 
GAAGCAGTGA TGGGGAAAAT TGCTCTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG 
GCGCGCAACA TCCAGAACGC TGGCGCCATT GGCGGCATTG TTATTGACGA CAATGAGGGG 
AGCAGCAGCG ATACCGCCCC TCTGTTCCAG ATGGCTGGGG ATGGGAAGGA CACAGACGAC 
ATCAAGATCC CCATGCTCTT CCTATTCAGC AAAGAAGGAA GCATCATCCT GGATGCTATT 
CGGGAATATG AGGACGTAGA AGTGCTCCTT TCTGATAAAG CAAAAGATCG AGATCCTGAG 
ATGGAAAGTG AAGAACAGCC ATCCTCTGAA AATGATCCTC CAAATCAGAG TGCCGAACAG 
ATCATCACAT CAAGTTCTCA GGACCCTGAT CTGGCTGAGC AAGCGGCTCC TGAAGAACGT 
GCTCTAAGCC CTCCCCCGGA ACCAGCGGGC GCACCATCTC CAGCAGAGAT GGACAATGCT 
GCAAACATTT CCCCCTCCGA ACAGAATTCC GACCCCACAG AAAACCACGA GAGCACAAGT 
CTCGGTGGTG AATGTACAGA TTTAGACGAC CAACCTCAAG AGCCATCAGA AACGGAGGAA 
GACTCCAATC CTAATGTTAG CTGGGGCCAA AAAGCCCAGC CTATAGACTC CATATTAGCA 
GACTGGAATG AAGATATAGA AGCTTTTGAA ATGATGGAGA AAGATGAACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004706733.1
CDS	192..3005
Translation	MSEAGGGPGWGFPVPQRAPWSLVAATAALCLVSATSVWPAGAAPMSREERQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTIDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNQSIPKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSIDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPYNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREYEDVEVLLSDKAKDRDPEMESEEQPSSENDPPNQSAEQIITSSSQDPDLAEQAAPEERALSPPPEPAGAPSPAEMDNAANISPSEQNSDPTENHESTSLGGECTDLDDQPQEPSETEEDSNPNVSWGQKAQPIDSILADWNEDIEAFEMMEKDEL

Target ORF information:

RefSeq Version	XM_004706676.1
Organism	Echinops telfairi(small Madagascar hedgehog)
Definition	Echinops telfairi ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004706676.1

ORF Insert Sequence:

ATGAGCGAAG CCGGCGGCGG CCCGGGCTGG GGGTTCCCGG TCCCCCAGCG AGCGCCATGG 
AGCCTGGTGG CGGCGACGGC CGCGCTCTGC CTGGTGTCAG CCACGTCCGT GTGGCCGGCG 
GGGGCCGCGC CCATGAGTCG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG 
TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCC 
TTAACCTGCA GAGGTCGAGT TCGAGGCCAG GAGCCAAGTC GCGGTGATGT TGATGATGCC 
TTGGGAAAAT TTTCTCTGAC ACTGATTGAT TCTTTGGACA CTCTCGTGGT ACTAAATAAA 
ACTAAAGAAT TTGAAGATGC TGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT 
GTAGTTGTAT CCGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCTTTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT 
CTCCAAATGG CAAAGCAATT GGGTTACAAA CTTCTACCAG CTTTCAATAC TACCAGTGGC 
CTTCCTTATC CAAGAATTAA TTTAAAGTTT GGGATCCGAA AACCAGAAGC TCGAACAGGA 
ACTGAGACAG ATACCTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT TGCTGCTTTA 
AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GGAAAGCTCT TGATTTTCTC 
TGGGAAAAAA GACAACGGAG TAGTAACTTA GTGGGTGTGA CTATCAATAT TCACACTGGA 
GACTGGGTCA GAAAAGATAG TGGAGTTGGA GCAGGAATTG ATTCTTATTA TGAATATCTG 
TTGAAAGCCT ATGTCTTGCT TGGGGATGAC AGTTTTCTGG AAAGATTTAA TACACACTAT 
GACGCCATAA TGAGGTACAT TAGCCAGCCT CCTCTACTAC TTGACGTGCA TATCCACAAA 
CCAATGCTGA ACGCTCGGAC GTGGATGGAT GCTCTCCTTG CCTTTTTCCC AGGCTTACAG 
GTTTTAAAGG GAGATATTAG ACCTGCAATT GAAACACATG AAATGTTATA TCAAGTGATT 
AAAAAACATA ATTTTCTACC AGAGGCATTT ACAATAGATT TCAGGGTACA CTGGGCTCAA 
CACCCTTTAA GGCCTGAGTT TGCAGAAAGC ACCTACTTCT TATATAAAGC TACCGGGGAT 
CCTTACTACC TTGAAGTAGG AAAAACACTT ATTGAAAATT TAAACAAATA TGCTAGAGTG 
CCTTGTGGAT TCGCTGCCAT GAAGGATGTT CGTACAGGAA GTCATGAGGA CAGAATGGAT 
TCTTTCTTTT TGGCTGAAAT GTTTAAATAC CTTTACCTAT TATTTGCGGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA TTACATTTTT ACCACAGAGG CTCATCTGTT ACCTCTGTGG 
CTCTCCACCA CAAATCAGAG CATCCCCAAG AAGAACACGA CTTCAGAATA TACAGAACTG 
GATGACAGTA ATTTTGATTG GACTTGTCCA AATACTCAGA TTCTCTTCCC TAATGACCCA 
TTGTATGCTC AGAGCATCCG TGAGCCCTTA AAAAATGTGG TGGACAAGAG CTGTCCTAGA 
GGCATCATTA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCTCCTCT GAGAGCCCGA 
GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCCTGA AGAAGATGGG GGTGAGTTTG 
ATTCACCTCA AAGACGGGAG AGTTCAGTTG GTCCAACATG CAATCCAAGC TGCAAGTTCC 
ATTGATGCTG AAGATGGGTT GAGGTTCATG CAGGAGATGA TTGAACTGTC AAGTCAGCAG 
CAGAAAGAAC AGCAGCTCCC CCCACGGGCT GTGCAGATTG TTTCCCACCC GTTTTTTGGC 
AGGGTCGTGT TGACCGCTGG GCCAGCTCAG TTTGGGCTGG ATCTGTCGAA GCATAAAGAG 
ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GCTCAGAACT TACCAACCCA 
GAAGCAGTGA TGGGGAAAAT TGCTCTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG 
GCGCGCAACA TCCAGAACGC TGGCGCCATT GGCGGCATTG TTATTGACGA CAATGAGGGG 
AGCAGCAGCG ATACCGCCCC TCTGTTCCAG ATGGCTGGGG ATGGGAAGGA CACAGACGAC 
ATCAAGATCC CCATGCTCTT CCTATTCAGC AAAGAAGGAA GCATCATCCT GGATGCTATT 
CGGGAATATG AGGACGTAGA AGTGCTCCTT TCTGATAAAG CAAAAGATCG AGATCCTGAG 
ATGGAAAGTG AAGAACAGCC ATCCTCTGAA AATGATCCTC CAAATCAGAG TGCCGAACAG 
ATCATCACAT CAAGTTCTCA GGACCCTGAT CTGGCTGAGC AAGCGGCTCC TGAAGAACGT 
GCTCTAAGCC CTCCCCCGGA ACCAGCGGGC GCACCATCTC CAGCAGAGAT GGACAATGCT 
GCAAACATTT CCCCCTCCGA ACAGAATTCC GACCCCACAG AAAACCACGA GAGCACAAGT 
CTCGGTGGTG AATGTACAGA TTTAGACGAC CAACCTCAAG AGCCATCAGA AACGGAGGAA 
GACTCCAATC CTAATGTTAG CTGGGGCCAA AAAGCCCAGC CTATAGACTC CATATTAGCA 
GACTGGAATG AAGATATAGA AGCTTTTGAA ATGATGGAGA AAGATGAACT ATGA