LOC108152913 cDNA ORF clone, Drosophila miranda

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108152913
Entrez Gene ID	108152913
Full Name	ecdysone-induced protein 74EF
Gene Type	protein-coding
Organism	Drosophila miranda

mRNA and Protein(s)

mRNA	Protein	Name
XM_017282590.1	XP_017138079.1	ecdysone-induced protein 74EF isoform X2
XM_017282589.1	XP_017138078.1	ecdysone-induced protein 74EF isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC108152913 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108152913 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODr82253	XM_017282589.1 Latest version!	Drosophila miranda ecdysone-induced protein 74EF (LOC108152913), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
ODr82259	XM_017282590.1 Latest version!	Drosophila miranda ecdysone-induced protein 74EF (LOC108152913), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108152913 ( XM_017282589.1 ) cDNA ORF clone, Drosophila miranda -> XP_017138078.1 Drosophila miranda ecdysone-induced protein 74EF (LOC108152913), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODr82253
Clone ID Related Accession (Same CDS sequence)	XM_017282589.1
Accession Version	XM_017282589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-15
Organism	Drosophila miranda
Product	ecdysone-induced protein 74EF isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030303.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Drosophila miranda Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTATGG TGCAACATTT GGTGGCTGCC ACGGCGCACA ATTTCGCCAG CCAGGCGGCA 
GCCCTAGCCA ATGTCTCGAG CTCCAGTTCG AGCTCTTCGC TGTCCTCCTC TTCATCCCTA 
TCCTCGTCAT TATCCTCCTC TTCGTCGTCG TCCTCCTCAT CCTCTTCGTC ATCCAGTTCG 
TCACTGTCCT CGGCCACGCC CACGCCTGTG GCATCACCAG CGGCAGCAGC TATGCCAGCT 
CCAGCAGAGG CCCCGCCAGC AGCAGGCGAG ACCCCAGAGG CAGAGGAGCC CATGGAGGAG 
GCTGCCAGCC AGGAAGAGCC CAAAACAGAG AAAGAGGAAC GCGATGAGCG TGAGAAGCTG 
GTGAAGCTGG AGAAGCAGGA ACGGGAACCA GAACCAGGTG CCATAGATGA TACCGAGTCA 
GGGTCGGCGC GGCAAAGTCC AAGTCCAGCT GCGTCTGCGT CTGCGTCCGC TGCCAAATCC 
CAGGAGCTAG CGCCAGCCCA AGAAGAGATC AGCGCCCCAG CCGTCGCCGT CGCCGTCGTC 
GCGGATGAGG AGGAGGAGGA GACCGAATCT CTCGCACCGG ACTGCCGCGA GTTCAAAGTG 
CTGTACAACC ATCTGAGGCA GCAGCAGCAC CACCAATCCT CCCCAGACAA AACTCGCAGC 
ACCCTGGACG ATGTCTCCAA GATCCTGTGG GAGCGCAAGC AGCAGCTCCA GCGGAGCTCC 
GTCATTACCA GCAACGTGCA GGCCGCCGCC GCAACCCAGC TCCTGCCGCA GCCACAGCAC 
AGGGATCCCG AGGAAAGCCA GCAGCAGCCC ATGAGCGACA TCGAGGACGA AGAGACCCTC 
GAGGATGCGG ACGAGGCCGA GGCCGATGCG GATGTCGAGG CGGATGCAGA CGACGAGGAG 
CTCCTCGAGC AGTACCAGGA CGGCTACGAC TCGCCCCTGG ATCTGTCCCT GAGCAGCGCC 
ACCAGCGCCG CCGCTAATGC AGCAGCAGCC GCCTCTGCGG CCAGTCGCCG GCGGGGACGC 
ACCTACTCCG GCACGGAGTC GGACGATTCG GCCCAGTGCG AGCGGGCGAG GATGCGCCTC 
AAGCCGGAGC GGAAGGCGGA GCGTTCGGCG GCCTACAAGA AGAGCTTGAT GAAGCGATAC 
TATACCGAAA TTCCTATTGT CAAACAATCG ACGAGTCCAG CACCACAACA ACAACAGCAA 
CAGCAGCAGC AGCATCAGCA GCAACAGATC CTGCAGCAGC AGCAACACCA GCAGCAGCAG 
CACAACGGAA CCGCCTTTGG TGGTGGCACC ACACTGCTGC ACATCAAGAC GGAGCAGAAC 
CCGTTGCTCA CGCCGCTCCA GCAGCAGCAG CAGCAGCAGC AACAGCAACA GCAGCATAGC 
TTACATGGTG GTGCAACGGG CGGCGGCACC AATGGTGGTG GCAATAACGG GAACAACAAC 
AACAGTGCAC ACCAGCAGCA GCAACAACCA CTGGCCATAC CGCACCGGCC CCTGTTGCAC 
AATCTGCTCA GCGGCGGCGC CATCCACAAT CCACATCACA GGAACTACAC GACGGCCACA 
ACAGGCTCAT TTCCGCCCAG TCCCGCGGAT AGCGGCGTAT CCGATGTGGA CAGCTCGAGT 
TCCGGCGGCC AGCCCTGCGC CGACGAGCTG AAGGCGCGCC TGGGCATGCC CGCTGCCACG 
TCAGCATCGG CAGCGGCCGC AGCAGCAGCA GCCGCGGCGG CAGCGGCGCA CCTCCACACG 
GGCACCTTTC TGCATCCGAA TTTATACCAG AACAATGCGG CGAATTCTCT GAGGAACATA 
TGGAATCGCA GTGTTGGGGT GCCCGACAAT TACTATGGCA GCAGTGGAGG CGGTAGCGGC 
GGCGGCGTCG GCAATCCAGG GGCGCCCGGC AACCCCCCGA CCCCCAGCTA CCTGACAACG 
TCCTACTTCA ACGCACCGAC GCAGCAGCAG CGAAGCTCTG CCGTCAACGG CTATCATTCC 
CTGCACCAGC AGCAGCAGCA GCAGACGCAG CAGCATCAAT CCCAGCAGCA GCAGCAACAA 
GCGTACGCGC AGCAGCAGCA GCAACAGCAA CAGCAGCACC CGCACTCGCA GCTGGCGCCT 
CCCTCGCATC CACATCCGCA TCCACACCAA ACGCAGCAGC AGCACACGCA TTCCACAATC 
GCCAATGCAG CGGCGGCAGC AGCGGCGGCC TCCGTTGTGA GCAGCAGCAG CAGCGCGGTG 
GCAGCGGCCG CCATGCTTAG CGCCAGTGCA GCGGCGGCGG CCGCCTCGCA GAGCGTCATC 
CAACCGGCGA CGTCGAGCGT CAGCTATGAT CTCTCCTACA TGCTGGAGCT GGGCGGCTTC 
CAGCAGCGGA AGGCGAAGAA GCCGCGCAAG CCGAAGCTGG AGATGGGGGT GAAGCGGCGC 
AGCCGGGAGG GCTCCACCAC CTACCTGTGG GAGTTCCTCC TGAAGCTGCT GCAGGATCGC 
GAGTACTGTC CTCGCTTCAT CAAGTGGACG AACCGGGAGA AGGGCGTCTT CAAGCTGGTC 
GACTCGAAGG CGGTGTCACG CCTCTGGGGC ATGCACAAGA ACAAGCCGGA CATGAACTAC 
GAGACGATGG GCCGTGCCCT ACGCTACTAC TACCAGCGCG GCATCCTTGC GAAGGTCGAT 
GGTCAGCGAC TGGTCTACCA GTTTGTCGAT GTGCCCAAGG ATATAGTTGA GATCGATTGC 
AATGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017138078.1
CDS	1107..3818
Translation	MIMVQHLVAATAHNFASQAAALANVSSSSSSSSLSSSSSLSSSLSSSSSSSSSSSSSSSSSLSSATPTPVASPAAAAMPAPAEAPPAAGETPEAEEPMEEAASQEEPKTEKEERDEREKLVKLEKQEREPEPGAIDDTESGSARQSPSPAASASASAAKSQELAPAQEEISAPAVAVAVVADEEEEETESLAPDCREFKVLYNHLRQQQHHQSSPDKTRSTLDDVSKILWERKQQLQRSSVITSNVQAAAATQLLPQPQHRDPEESQQQPMSDIEDEETLEDADEAEADADVEADADDEELLEQYQDGYDSPLDLSLSSATSAAANAAAAASAASRRRGRTYSGTESDDSAQCERARMRLKPERKAERSAAYKKSLMKRYYTEIPIVKQSTSPAPQQQQQQQQQHQQQQILQQQQHQQQQHNGTAFGGGTTLLHIKTEQNPLLTPLQQQQQQQQQQQQHSLHGGATGGGTNGGGNNGNNNNSAHQQQQQPLAIPHRPLLHNLLSGGAIHNPHHRNYTTATTGSFPPSPADSGVSDVDSSSSGGQPCADELKARLGMPAATSASAAAAAAAAAAAAAHLHTGTFLHPNLYQNNAANSLRNIWNRSVGVPDNYYGSSGGGSGGGVGNPGAPGNPPTPSYLTTSYFNAPTQQQRSSAVNGYHSLHQQQQQQTQQHQSQQQQQQAYAQQQQQQQQQHPHSQLAPPSHPHPHPHQTQQQHTHSTIANAAAAAAAASVVSSSSSAVAAAAMLSASAAAAAASQSVIQPATSSVSYDLSYMLELGGFQQRKAKKPRKPKLEMGVKRRSREGSTTYLWEFLLKLLQDREYCPRFIKWTNREKGVFKLVDSKAVSRLWGMHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVYQFVDVPKDIVEIDCNGV

Target ORF information:

RefSeq Version	XM_017282589.1
Organism	Drosophila miranda
Definition	Drosophila miranda ecdysone-induced protein 74EF (LOC108152913), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017282589.1

ORF Insert Sequence:

ATGATTATGG TGCAACATTT GGTGGCTGCC ACGGCGCACA ATTTCGCCAG CCAGGCGGCA 
GCCCTAGCCA ATGTCTCGAG CTCCAGTTCG AGCTCTTCGC TGTCCTCCTC TTCATCCCTA 
TCCTCGTCAT TATCCTCCTC TTCGTCGTCG TCCTCCTCAT CCTCTTCGTC ATCCAGTTCG 
TCACTGTCCT CGGCCACGCC CACGCCTGTG GCATCACCAG CGGCAGCAGC TATGCCAGCT 
CCAGCAGAGG CCCCGCCAGC AGCAGGCGAG ACCCCAGAGG CAGAGGAGCC CATGGAGGAG 
GCTGCCAGCC AGGAAGAGCC CAAAACAGAG AAAGAGGAAC GCGATGAGCG TGAGAAGCTG 
GTGAAGCTGG AGAAGCAGGA ACGGGAACCA GAACCAGGTG CCATAGATGA TACCGAGTCA 
GGGTCGGCGC GGCAAAGTCC AAGTCCAGCT GCGTCTGCGT CTGCGTCCGC TGCCAAATCC 
CAGGAGCTAG CGCCAGCCCA AGAAGAGATC AGCGCCCCAG CCGTCGCCGT CGCCGTCGTC 
GCGGATGAGG AGGAGGAGGA GACCGAATCT CTCGCACCGG ACTGCCGCGA GTTCAAAGTG 
CTGTACAACC ATCTGAGGCA GCAGCAGCAC CACCAATCCT CCCCAGACAA AACTCGCAGC 
ACCCTGGACG ATGTCTCCAA GATCCTGTGG GAGCGCAAGC AGCAGCTCCA GCGGAGCTCC 
GTCATTACCA GCAACGTGCA GGCCGCCGCC GCAACCCAGC TCCTGCCGCA GCCACAGCAC 
AGGGATCCCG AGGAAAGCCA GCAGCAGCCC ATGAGCGACA TCGAGGACGA AGAGACCCTC 
GAGGATGCGG ACGAGGCCGA GGCCGATGCG GATGTCGAGG CGGATGCAGA CGACGAGGAG 
CTCCTCGAGC AGTACCAGGA CGGCTACGAC TCGCCCCTGG ATCTGTCCCT GAGCAGCGCC 
ACCAGCGCCG CCGCTAATGC AGCAGCAGCC GCCTCTGCGG CCAGTCGCCG GCGGGGACGC 
ACCTACTCCG GCACGGAGTC GGACGATTCG GCCCAGTGCG AGCGGGCGAG GATGCGCCTC 
AAGCCGGAGC GGAAGGCGGA GCGTTCGGCG GCCTACAAGA AGAGCTTGAT GAAGCGATAC 
TATACCGAAA TTCCTATTGT CAAACAATCG ACGAGTCCAG CACCACAACA ACAACAGCAA 
CAGCAGCAGC AGCATCAGCA GCAACAGATC CTGCAGCAGC AGCAACACCA GCAGCAGCAG 
CACAACGGAA CCGCCTTTGG TGGTGGCACC ACACTGCTGC ACATCAAGAC GGAGCAGAAC 
CCGTTGCTCA CGCCGCTCCA GCAGCAGCAG CAGCAGCAGC AACAGCAACA GCAGCATAGC 
TTACATGGTG GTGCAACGGG CGGCGGCACC AATGGTGGTG GCAATAACGG GAACAACAAC 
AACAGTGCAC ACCAGCAGCA GCAACAACCA CTGGCCATAC CGCACCGGCC CCTGTTGCAC 
AATCTGCTCA GCGGCGGCGC CATCCACAAT CCACATCACA GGAACTACAC GACGGCCACA 
ACAGGCTCAT TTCCGCCCAG TCCCGCGGAT AGCGGCGTAT CCGATGTGGA CAGCTCGAGT 
TCCGGCGGCC AGCCCTGCGC CGACGAGCTG AAGGCGCGCC TGGGCATGCC CGCTGCCACG 
TCAGCATCGG CAGCGGCCGC AGCAGCAGCA GCCGCGGCGG CAGCGGCGCA CCTCCACACG 
GGCACCTTTC TGCATCCGAA TTTATACCAG AACAATGCGG CGAATTCTCT GAGGAACATA 
TGGAATCGCA GTGTTGGGGT GCCCGACAAT TACTATGGCA GCAGTGGAGG CGGTAGCGGC 
GGCGGCGTCG GCAATCCAGG GGCGCCCGGC AACCCCCCGA CCCCCAGCTA CCTGACAACG 
TCCTACTTCA ACGCACCGAC GCAGCAGCAG CGAAGCTCTG CCGTCAACGG CTATCATTCC 
CTGCACCAGC AGCAGCAGCA GCAGACGCAG CAGCATCAAT CCCAGCAGCA GCAGCAACAA 
GCGTACGCGC AGCAGCAGCA GCAACAGCAA CAGCAGCACC CGCACTCGCA GCTGGCGCCT 
CCCTCGCATC CACATCCGCA TCCACACCAA ACGCAGCAGC AGCACACGCA TTCCACAATC 
GCCAATGCAG CGGCGGCAGC AGCGGCGGCC TCCGTTGTGA GCAGCAGCAG CAGCGCGGTG 
GCAGCGGCCG CCATGCTTAG CGCCAGTGCA GCGGCGGCGG CCGCCTCGCA GAGCGTCATC 
CAACCGGCGA CGTCGAGCGT CAGCTATGAT CTCTCCTACA TGCTGGAGCT GGGCGGCTTC 
CAGCAGCGGA AGGCGAAGAA GCCGCGCAAG CCGAAGCTGG AGATGGGGGT GAAGCGGCGC 
AGCCGGGAGG GCTCCACCAC CTACCTGTGG GAGTTCCTCC TGAAGCTGCT GCAGGATCGC 
GAGTACTGTC CTCGCTTCAT CAAGTGGACG AACCGGGAGA AGGGCGTCTT CAAGCTGGTC 
GACTCGAAGG CGGTGTCACG CCTCTGGGGC ATGCACAAGA ACAAGCCGGA CATGAACTAC 
GAGACGATGG GCCGTGCCCT ACGCTACTAC TACCAGCGCG GCATCCTTGC GAAGGTCGAT 
GGTCAGCGAC TGGTCTACCA GTTTGTCGAT GTGCCCAAGG ATATAGTTGA GATCGATTGC 
AATGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108152913 ( XM_017282590.1 ) cDNA ORF clone, Drosophila miranda -> XP_017138079.1 Drosophila miranda ecdysone-induced protein 74EF (LOC108152913), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODr82259
Clone ID Related Accession (Same CDS sequence)	XM_017282590.1
Accession Version	XM_017282590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-15
Organism	Drosophila miranda
Product	ecdysone-induced protein 74EF isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030303.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Drosophila miranda Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTTA TCGACGATGC ACTGCTCTGG TGTCCGGATA ACGATGGTCG TCTTGTCGGT 
GGCTTAGATC TGGCAACATG CATAGCCGAT GATTCAGCGG TGAATGGAAC GGAAGGCATC 
AATCCCACAA TCCAATCGGC TGGCAATCCC AACAATCAAC AGCAACAGGG CGTAGGAATC 
GGCGGAATTG GAGGCTCTAC GAATATCGGA GTTGGATGTG GAGTTGGCGG GGAACTGAAT 
GGATCGGCGG CACGCAGCGT CAATGTGGTG GTGGAGCCGC TATGCGGCAG CGACTCCTCC 
GACGAGCTCT TCCGCAGCTT CTCCGAGAGC AACTTTGAGA TCGAGAGCCT GCTGTCCGAT 
TTGGCCACCG TTGAGGTGAA AGTCGAAAAT GAGGAGAACA ACAATAACGT CATCACTGCC 
GCCGATGTGG TGGACGACGA TGACTTTGCC AGCGTCGCCG CCGCCGTGGT GGCCGGCGAC 
GATTTGCTAG CCAAAGAGAA CGCCCAACTG AGCGCCCAGG GCCTGGTCGA CTCGGTGGCC 
GCCAGTCTGG GCGATGGTGG CGACGCCAAT GGCCAGCAGG CGCTTCTGGC CTTCGGCTCC 
TCCAACTCGG CGGCGGCGGC GGCCGTGGCA GCTGCTGCAG CCGCTCTCTG CGGCGATCTG 
ATCAACAACA ATAGCAATGG AGGAGGAGGA GGCGGCGCAG TGGGTGGCGG TGACTGCTCC 
ACCAAGCTGG AGTACGCCTT GATGGGCGGC CAGCCTCATG CGGAGGAGCC CCGCTTCGTG 
ACCAGCGCCG CAGCGAATCC CCTGCTCGTG GAGAAGCTCA TGTCCAAGTG CCTAAACATC 
GAGAAGCGGC TGGATAAGAT GGGCGATACC GAAATTCCTA TTGTCAAACA ATCGACGAGT 
CCAGCACCAC AACAACAACA GCAACAGCAG CAGCAGCATC AGCAGCAACA GATCCTGCAG 
CAGCAGCAAC ACCAGCAGCA GCAGCACAAC GGAACCGCCT TTGGTGGTGG CACCACACTG 
CTGCACATCA AGACGGAGCA GAACCCGTTG CTCACGCCGC TCCAGCAGCA GCAGCAGCAG 
CAGCAACAGC AACAGCAGCA TAGCTTACAT GGTGGTGCAA CGGGCGGCGG CACCAATGGT 
GGTGGCAATA ACGGGAACAA CAACAACAGT GCACACCAGC AGCAGCAACA ACCACTGGCC 
ATACCGCACC GGCCCCTGTT GCACAATCTG CTCAGCGGCG GCGCCATCCA CAATCCACAT 
CACAGGAACT ACACGACGGC CACAACAGGC TCATTTCCGC CCAGTCCCGC GGATAGCGGC 
GTATCCGATG TGGACAGCTC GAGTTCCGGC GGCCAGCCCT GCGCCGACGA GCTGAAGGCG 
CGCCTGGGCA TGCCCGCTGC CACGTCAGCA TCGGCAGCGG CCGCAGCAGC AGCAGCCGCG 
GCGGCAGCGG CGCACCTCCA CACGGGCACC TTTCTGCATC CGAATTTATA CCAGAACAAT 
GCGGCGAATT CTCTGAGGAA CATATGGAAT CGCAGTGTTG GGGTGCCCGA CAATTACTAT 
GGCAGCAGTG GAGGCGGTAG CGGCGGCGGC GTCGGCAATC CAGGGGCGCC CGGCAACCCC 
CCGACCCCCA GCTACCTGAC AACGTCCTAC TTCAACGCAC CGACGCAGCA GCAGCGAAGC 
TCTGCCGTCA ACGGCTATCA TTCCCTGCAC CAGCAGCAGC AGCAGCAGAC GCAGCAGCAT 
CAATCCCAGC AGCAGCAGCA ACAAGCGTAC GCGCAGCAGC AGCAGCAACA GCAACAGCAG 
CACCCGCACT CGCAGCTGGC GCCTCCCTCG CATCCACATC CGCATCCACA CCAAACGCAG 
CAGCAGCACA CGCATTCCAC AATCGCCAAT GCAGCGGCGG CAGCAGCGGC GGCCTCCGTT 
GTGAGCAGCA GCAGCAGCGC GGTGGCAGCG GCCGCCATGC TTAGCGCCAG TGCAGCGGCG 
GCGGCCGCCT CGCAGAGCGT CATCCAACCG GCGACGTCGA GCGTCAGCTA TGATCTCTCC 
TACATGCTGG AGCTGGGCGG CTTCCAGCAG CGGAAGGCGA AGAAGCCGCG CAAGCCGAAG 
CTGGAGATGG GGGTGAAGCG GCGCAGCCGG GAGGGCTCCA CCACCTACCT GTGGGAGTTC 
CTCCTGAAGC TGCTGCAGGA TCGCGAGTAC TGTCCTCGCT TCATCAAGTG GACGAACCGG 
GAGAAGGGCG TCTTCAAGCT GGTCGACTCG AAGGCGGTGT CACGCCTCTG GGGCATGCAC 
AAGAACAAGC CGGACATGAA CTACGAGACG ATGGGCCGTG CCCTACGCTA CTACTACCAG 
CGCGGCATCC TTGCGAAGGT CGATGGTCAG CGACTGGTCT ACCAGTTTGT CGATGTGCCC 
AAGGATATAG TTGAGATCGA TTGCAATGGT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017138079.1
CDS	349..2784
Translation	MPFIDDALLWCPDNDGRLVGGLDLATCIADDSAVNGTEGINPTIQSAGNPNNQQQQGVGIGGIGGSTNIGVGCGVGGELNGSAARSVNVVVEPLCGSDSSDELFRSFSESNFEIESLLSDLATVEVKVENEENNNNVITAADVVDDDDFASVAAAVVAGDDLLAKENAQLSAQGLVDSVAASLGDGGDANGQQALLAFGSSNSAAAAAVAAAAAALCGDLINNNSNGGGGGGAVGGGDCSTKLEYALMGGQPHAEEPRFVTSAAANPLLVEKLMSKCLNIEKRLDKMGDTEIPIVKQSTSPAPQQQQQQQQQHQQQQILQQQQHQQQQHNGTAFGGGTTLLHIKTEQNPLLTPLQQQQQQQQQQQQHSLHGGATGGGTNGGGNNGNNNNSAHQQQQQPLAIPHRPLLHNLLSGGAIHNPHHRNYTTATTGSFPPSPADSGVSDVDSSSSGGQPCADELKARLGMPAATSASAAAAAAAAAAAAAHLHTGTFLHPNLYQNNAANSLRNIWNRSVGVPDNYYGSSGGGSGGGVGNPGAPGNPPTPSYLTTSYFNAPTQQQRSSAVNGYHSLHQQQQQQTQQHQSQQQQQQAYAQQQQQQQQQHPHSQLAPPSHPHPHPHQTQQQHTHSTIANAAAAAAAASVVSSSSSAVAAAAMLSASAAAAAASQSVIQPATSSVSYDLSYMLELGGFQQRKAKKPRKPKLEMGVKRRSREGSTTYLWEFLLKLLQDREYCPRFIKWTNREKGVFKLVDSKAVSRLWGMHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVYQFVDVPKDIVEIDCNGV

Target ORF information:

RefSeq Version	XM_017282590.1
Organism	Drosophila miranda
Definition	Drosophila miranda ecdysone-induced protein 74EF (LOC108152913), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017282590.1

ORF Insert Sequence:

ATGCCCTTTA TCGACGATGC ACTGCTCTGG TGTCCGGATA ACGATGGTCG TCTTGTCGGT 
GGCTTAGATC TGGCAACATG CATAGCCGAT GATTCAGCGG TGAATGGAAC GGAAGGCATC 
AATCCCACAA TCCAATCGGC TGGCAATCCC AACAATCAAC AGCAACAGGG CGTAGGAATC 
GGCGGAATTG GAGGCTCTAC GAATATCGGA GTTGGATGTG GAGTTGGCGG GGAACTGAAT 
GGATCGGCGG CACGCAGCGT CAATGTGGTG GTGGAGCCGC TATGCGGCAG CGACTCCTCC 
GACGAGCTCT TCCGCAGCTT CTCCGAGAGC AACTTTGAGA TCGAGAGCCT GCTGTCCGAT 
TTGGCCACCG TTGAGGTGAA AGTCGAAAAT GAGGAGAACA ACAATAACGT CATCACTGCC 
GCCGATGTGG TGGACGACGA TGACTTTGCC AGCGTCGCCG CCGCCGTGGT GGCCGGCGAC 
GATTTGCTAG CCAAAGAGAA CGCCCAACTG AGCGCCCAGG GCCTGGTCGA CTCGGTGGCC 
GCCAGTCTGG GCGATGGTGG CGACGCCAAT GGCCAGCAGG CGCTTCTGGC CTTCGGCTCC 
TCCAACTCGG CGGCGGCGGC GGCCGTGGCA GCTGCTGCAG CCGCTCTCTG CGGCGATCTG 
ATCAACAACA ATAGCAATGG AGGAGGAGGA GGCGGCGCAG TGGGTGGCGG TGACTGCTCC 
ACCAAGCTGG AGTACGCCTT GATGGGCGGC CAGCCTCATG CGGAGGAGCC CCGCTTCGTG 
ACCAGCGCCG CAGCGAATCC CCTGCTCGTG GAGAAGCTCA TGTCCAAGTG CCTAAACATC 
GAGAAGCGGC TGGATAAGAT GGGCGATACC GAAATTCCTA TTGTCAAACA ATCGACGAGT 
CCAGCACCAC AACAACAACA GCAACAGCAG CAGCAGCATC AGCAGCAACA GATCCTGCAG 
CAGCAGCAAC ACCAGCAGCA GCAGCACAAC GGAACCGCCT TTGGTGGTGG CACCACACTG 
CTGCACATCA AGACGGAGCA GAACCCGTTG CTCACGCCGC TCCAGCAGCA GCAGCAGCAG 
CAGCAACAGC AACAGCAGCA TAGCTTACAT GGTGGTGCAA CGGGCGGCGG CACCAATGGT 
GGTGGCAATA ACGGGAACAA CAACAACAGT GCACACCAGC AGCAGCAACA ACCACTGGCC 
ATACCGCACC GGCCCCTGTT GCACAATCTG CTCAGCGGCG GCGCCATCCA CAATCCACAT 
CACAGGAACT ACACGACGGC CACAACAGGC TCATTTCCGC CCAGTCCCGC GGATAGCGGC 
GTATCCGATG TGGACAGCTC GAGTTCCGGC GGCCAGCCCT GCGCCGACGA GCTGAAGGCG 
CGCCTGGGCA TGCCCGCTGC CACGTCAGCA TCGGCAGCGG CCGCAGCAGC AGCAGCCGCG 
GCGGCAGCGG CGCACCTCCA CACGGGCACC TTTCTGCATC CGAATTTATA CCAGAACAAT 
GCGGCGAATT CTCTGAGGAA CATATGGAAT CGCAGTGTTG GGGTGCCCGA CAATTACTAT 
GGCAGCAGTG GAGGCGGTAG CGGCGGCGGC GTCGGCAATC CAGGGGCGCC CGGCAACCCC 
CCGACCCCCA GCTACCTGAC AACGTCCTAC TTCAACGCAC CGACGCAGCA GCAGCGAAGC 
TCTGCCGTCA ACGGCTATCA TTCCCTGCAC CAGCAGCAGC AGCAGCAGAC GCAGCAGCAT 
CAATCCCAGC AGCAGCAGCA ACAAGCGTAC GCGCAGCAGC AGCAGCAACA GCAACAGCAG 
CACCCGCACT CGCAGCTGGC GCCTCCCTCG CATCCACATC CGCATCCACA CCAAACGCAG 
CAGCAGCACA CGCATTCCAC AATCGCCAAT GCAGCGGCGG CAGCAGCGGC GGCCTCCGTT 
GTGAGCAGCA GCAGCAGCGC GGTGGCAGCG GCCGCCATGC TTAGCGCCAG TGCAGCGGCG 
GCGGCCGCCT CGCAGAGCGT CATCCAACCG GCGACGTCGA GCGTCAGCTA TGATCTCTCC 
TACATGCTGG AGCTGGGCGG CTTCCAGCAG CGGAAGGCGA AGAAGCCGCG CAAGCCGAAG 
CTGGAGATGG GGGTGAAGCG GCGCAGCCGG GAGGGCTCCA CCACCTACCT GTGGGAGTTC 
CTCCTGAAGC TGCTGCAGGA TCGCGAGTAC TGTCCTCGCT TCATCAAGTG GACGAACCGG 
GAGAAGGGCG TCTTCAAGCT GGTCGACTCG AAGGCGGTGT CACGCCTCTG GGGCATGCAC 
AAGAACAAGC CGGACATGAA CTACGAGACG ATGGGCCGTG CCCTACGCTA CTACTACCAG 
CGCGGCATCC TTGCGAAGGT CGATGGTCAG CGACTGGTCT ACCAGTTTGT CGATGTGCCC 
AAGGATATAG TTGAGATCGA TTGCAATGGT GTCTGA