bsk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bsk
Entrez Gene ID	44801
Full Name	basket
Synonyms	BSK,Bsk,CG5680,D-JNK,D-junk,DBSK/JNK,DJNK,DJNK/bsk,Dmel\CG5680,JNK,JNK/SAPK,Jnk,Junk,SAPKa,dJNK,jnk,pJNK
General protein information	Preferred Names basket Names CG5680 gene product from transcript CG5680-RB CG5680-PB CG5680-PE CG5680-PF JNK JUN kinase Jun N-terminal kinase Jun NH2-terminal kinase Jun-N terminal kinase Jun-N-terminal kinase Jun-kinase bsk-PB bsk-PE bsk-PF c-Jun N-terminal kinase c-Jun aminoterminal kinase c-Jun-N-terminal kinase pJNK
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31B1-31B1\|2-33 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_164900.3	NP_723541.1	basket, isoform B
NM_001169461.3	NP_001162932.1	basket, isoform E
NM_001169459.1	NP_001162930.1	basket, isoform F

Pathways from BioSystems

Homologies

	MAPK8	XP_001108815.1
Canis lupus familiaris (dog)	MAPK8	XP_005637524.1
Rattus norvegicus (Norway rat)	Mapk8	NP_446281.1
Xenopus tropicalis (tropical clawed frog)	mapk8	NP_001123415.1
Homo sapiens (human)	MAPK8	NP_001265476.1
Bos taurus (cattle)	MAPK8	NP_001179903.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009461	XP_310236.3
Pan troglodytes (chimpanzee)	MAPK8	XP_001136927.1
Gallus gallus (chicken)	MAPK8	XP_001233169.1
Danio rerio (zebrafish)	mapk8a	NP_001103859.1
Drosophila melanogaster (fruit fly)	bsk	NP_001162930.1
Caenorhabditis elegans (roundworm)	jnk-1	NP_001021270.1
Mus musculus (house mouse)	Mapk8	NP_057909.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased expression of the Drosophila JNK basket in the setting of reduced cindr expression was found to result in even more severe apoptosis, whilst ectopic death was found to be reduced if retinas were heterozygous for basket.
Title: JNK is antagonized to ensure the correct number of interommatidial cells pattern the Drosophila retina.

Data show that JNK signalling inhibits the growth of losers, while JAK/STAT signalling promotes competition-induced winner cell proliferation.
Title: Chronic activation of JNK JAK/STAT and oxidative stress signalling causes the loser cell status.

Here we uncover a cell non-autonomous requirement for the Epidermal growth factor receptor (Egfr) pathway in the lateral epidermis for sustained dpp expression in the LE. Specifically, we demonstrate that Egfr pathway activity in the lateral epidermis prevents expression of the gene scarface (scaf), encoding a secreted antagonist of JNK signaling
Title: Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure.

n addition to significantly increasing the number of JNK target genes identified so far, our results reveal that the LE is a highly heterogeneous morphogenetic organizer, sculpted through crosstalk between JNK, segmental and AP signalling. This fine-tuning regulatory mechanism is essential to coordinate morphogenesis and dynamics of tissue sealing
Title: Signalling crosstalk at the leading edge controls tissue closure dynamics in the Drosophila embryo.

aken together, these results reveal that inactivation of Rpd3 independently regulates JNK and Yki activities and that both Hippo and JNK signaling pathways contribute to Rpd3 RNAi-induced apoptosis.
Title: Loss of histone deacetylase HDAC1 induces cell death in Drosophila epithelial cells through JNK and Hippo signaling.

The following bsk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bsk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15210	NM_001169459.1 Latest version!	Drosophila melanogaster basket (bsk), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15210	NM_001169461.3 Latest version!	Drosophila melanogaster basket (bsk), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15210	NM_164900.3 Latest version!	Drosophila melanogaster basket (bsk), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bsk ( NM_164900.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723541.1 Drosophila melanogaster basket (bsk), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15210
Clone ID Related Accession (Same CDS sequence)	NM_164900.3 , NM_001169461.3 , NM_001169459.1
Accession Version	NM_164900.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	basket, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164900.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACAG CTCAGCACCA ACACTACACC GTCGAGGTGG GGGACACCAA CTTCACCATC 
CACAGTCGGT ACATTAATCT ACGGCCCATA GGATCAGGTG CCCAAGGAAT AGTATGCGCC 
GCTTACGATA CTATCACCCA GCAAAATGTG GCGATTAAGA AACTATCTCG ACCATTCCAA 
AATGTAACCC ATGCCAAGCG AGCATATAGG GAATTCAAGC TAATGAAGCT TGTTAACCAT 
AAAAACATAA TTGGCTTACT TAATGCTTTT ACGCCGCAAA GGAACTTGGA AGAGTTTCAG 
GATGTCTACC TGGTCATGGA GCTGATGGAC GCTAATCTCT GCCAGGTCAT CCAAATGGAC 
TTGGATCACG ACAGAATGTC CTATTTGCTT TATCAAATGT TGTGCGGAAT AAAACATTTA 
CACTCAGCAG GAATTATTCA CAGAGACTTA AAGCCATCCA ATATAGTTGT AAAGGCCGAC 
TGCACTCTAA AAATTTTGGA TTTCGGTCTG GCACGTACTG CAGGAACTAC CTTTATGATG 
ACTCCCTATG TGGTCACCCG ATATTACAGG GCTCCAGAGG TCATTTTGGG CATGGGATAC 
ACAGAGAACG TGGACATATG GTCCGTCGGT TGCATTATGG GCGAAATGAT ACGGGGCGGT 
GTGCTGTTTC CTGGAACGGA TCATATAGAT CAGTGGAATA AAATTATTGA GCAATTAGGT 
ACACCATCAC CCTCGTTTAT GCAACGGTTA CAGCCAACCG TCCGAAACTA TGTGGAAAAT 
CGTCCTAGAT ACACTGGATA CTCATTTGAT AGACTTTTCC CCGATGGCTT GTTTCCAAAT 
GATAATAATC AGAATAGCCG CCGGAAGGCT TCTGACGCTA GAAATCTTCT TAGTAAAATG 
CTAGTCATTG ATCCAGAGCA GCGGATATCT GTGGACGAGG CTTTGAAGCA TGAATATATC 
AACGTGTGGT ATGATGCTGA GGAAGTGGAT GCTCCCGCTC CGGAGCCATA TGATCACAGC 
GTGGACGAAA GGGAACACAC TGTGGAGCAG TGGAAGGAGC TAATCTATGA GGAAGTCATG 
GACTACGAAG CACACAATAC AAATAATAGA ACGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723541.1
CDS	271..1389
Translation	MTTAQHQHYTVEVGDTNFTIHSRYINLRPIGSGAQGIVCAAYDTITQQNVAIKKLSRPFQNVTHAKRAYREFKLMKLVNHKNIIGLLNAFTPQRNLEEFQDVYLVMELMDANLCQVIQMDLDHDRMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKADCTLKILDFGLARTAGTTFMMTPYVVTRYYRAPEVILGMGYTENVDIWSVGCIMGEMIRGGVLFPGTDHIDQWNKIIEQLGTPSPSFMQRLQPTVRNYVENRPRYTGYSFDRLFPDGLFPNDNNQNSRRKASDARNLLSKMLVIDPEQRISVDEALKHEYINVWYDAEEVDAPAPEPYDHSVDEREHTVEQWKELIYEEVMDYEAHNTNNRTR

Target ORF information:

RefSeq Version	NM_164900.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster basket (bsk), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164900.3

ORF Insert Sequence:

ATGACGACAG CTCAGCACCA ACACTACACC GTCGAGGTGG GGGACACCAA CTTCACCATC 
CACAGTCGGT ACATTAATCT ACGGCCCATA GGATCAGGTG CCCAAGGAAT AGTATGCGCC 
GCTTACGATA CTATCACCCA GCAAAATGTG GCGATTAAGA AACTATCTCG ACCATTCCAA 
AATGTAACCC ATGCCAAGCG AGCATATAGG GAATTCAAGC TAATGAAGCT TGTTAACCAT 
AAAAACATAA TTGGCTTACT TAATGCTTTT ACGCCGCAAA GGAACTTGGA AGAGTTTCAG 
GATGTCTACC TGGTCATGGA GCTGATGGAC GCTAATCTCT GCCAGGTCAT CCAAATGGAC 
TTGGATCACG ACAGAATGTC CTATTTGCTT TATCAAATGT TGTGCGGAAT AAAACATTTA 
CACTCAGCAG GAATTATTCA CAGAGACTTA AAGCCATCCA ATATAGTTGT AAAGGCCGAC 
TGCACTCTAA AAATTTTGGA TTTCGGTCTG GCACGTACTG CAGGAACTAC CTTTATGATG 
ACTCCCTATG TGGTCACCCG ATATTACAGG GCTCCAGAGG TCATTTTGGG CATGGGATAC 
ACAGAGAACG TGGACATATG GTCCGTCGGT TGCATTATGG GCGAAATGAT ACGGGGCGGT 
GTGCTGTTTC CTGGAACGGA TCATATAGAT CAGTGGAATA AAATTATTGA GCAATTAGGT 
ACACCATCAC CCTCGTTTAT GCAACGGTTA CAGCCAACCG TCCGAAACTA TGTGGAAAAT 
CGTCCTAGAT ACACTGGATA CTCATTTGAT AGACTTTTCC CCGATGGCTT GTTTCCAAAT 
GATAATAATC AGAATAGCCG CCGGAAGGCT TCTGACGCTA GAAATCTTCT TAGTAAAATG 
CTAGTCATTG ATCCAGAGCA GCGGATATCT GTGGACGAGG CTTTGAAGCA TGAATATATC 
AACGTGTGGT ATGATGCTGA GGAAGTGGAT GCTCCCGCTC CGGAGCCATA TGATCACAGC 
GTGGACGAAA GGGAACACAC TGTGGAGCAG TGGAAGGAGC TAATCTATGA GGAAGTCATG 
GACTACGAAG CACACAATAC AAATAATAGA ACGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bsk ( NM_001169461.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162932.1 Drosophila melanogaster basket (bsk), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa15210
Clone ID Related Accession (Same CDS sequence)	NM_164900.3 , NM_001169461.3 , NM_001169459.1
Accession Version	NM_001169461.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	basket, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169461.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACAG CTCAGCACCA ACACTACACC GTCGAGGTGG GGGACACCAA CTTCACCATC 
CACAGTCGGT ACATTAATCT ACGGCCCATA GGATCAGGTG CCCAAGGAAT AGTATGCGCC 
GCTTACGATA CTATCACCCA GCAAAATGTG GCGATTAAGA AACTATCTCG ACCATTCCAA 
AATGTAACCC ATGCCAAGCG AGCATATAGG GAATTCAAGC TAATGAAGCT TGTTAACCAT 
AAAAACATAA TTGGCTTACT TAATGCTTTT ACGCCGCAAA GGAACTTGGA AGAGTTTCAG 
GATGTCTACC TGGTCATGGA GCTGATGGAC GCTAATCTCT GCCAGGTCAT CCAAATGGAC 
TTGGATCACG ACAGAATGTC CTATTTGCTT TATCAAATGT TGTGCGGAAT AAAACATTTA 
CACTCAGCAG GAATTATTCA CAGAGACTTA AAGCCATCCA ATATAGTTGT AAAGGCCGAC 
TGCACTCTAA AAATTTTGGA TTTCGGTCTG GCACGTACTG CAGGAACTAC CTTTATGATG 
ACTCCCTATG TGGTCACCCG ATATTACAGG GCTCCAGAGG TCATTTTGGG CATGGGATAC 
ACAGAGAACG TGGACATATG GTCCGTCGGT TGCATTATGG GCGAAATGAT ACGGGGCGGT 
GTGCTGTTTC CTGGAACGGA TCATATAGAT CAGTGGAATA AAATTATTGA GCAATTAGGT 
ACACCATCAC CCTCGTTTAT GCAACGGTTA CAGCCAACCG TCCGAAACTA TGTGGAAAAT 
CGTCCTAGAT ACACTGGATA CTCATTTGAT AGACTTTTCC CCGATGGCTT GTTTCCAAAT 
GATAATAATC AGAATAGCCG CCGGAAGGCT TCTGACGCTA GAAATCTTCT TAGTAAAATG 
CTAGTCATTG ATCCAGAGCA GCGGATATCT GTGGACGAGG CTTTGAAGCA TGAATATATC 
AACGTGTGGT ATGATGCTGA GGAAGTGGAT GCTCCCGCTC CGGAGCCATA TGATCACAGC 
GTGGACGAAA GGGAACACAC TGTGGAGCAG TGGAAGGAGC TAATCTATGA GGAAGTCATG 
GACTACGAAG CACACAATAC AAATAATAGA ACGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162932.1
CDS	391..1509
Translation	MTTAQHQHYTVEVGDTNFTIHSRYINLRPIGSGAQGIVCAAYDTITQQNVAIKKLSRPFQNVTHAKRAYREFKLMKLVNHKNIIGLLNAFTPQRNLEEFQDVYLVMELMDANLCQVIQMDLDHDRMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKADCTLKILDFGLARTAGTTFMMTPYVVTRYYRAPEVILGMGYTENVDIWSVGCIMGEMIRGGVLFPGTDHIDQWNKIIEQLGTPSPSFMQRLQPTVRNYVENRPRYTGYSFDRLFPDGLFPNDNNQNSRRKASDARNLLSKMLVIDPEQRISVDEALKHEYINVWYDAEEVDAPAPEPYDHSVDEREHTVEQWKELIYEEVMDYEAHNTNNRTR

Target ORF information:

RefSeq Version	NM_001169461.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster basket (bsk), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169461.3

ORF Insert Sequence:

ATGACGACAG CTCAGCACCA ACACTACACC GTCGAGGTGG GGGACACCAA CTTCACCATC 
CACAGTCGGT ACATTAATCT ACGGCCCATA GGATCAGGTG CCCAAGGAAT AGTATGCGCC 
GCTTACGATA CTATCACCCA GCAAAATGTG GCGATTAAGA AACTATCTCG ACCATTCCAA 
AATGTAACCC ATGCCAAGCG AGCATATAGG GAATTCAAGC TAATGAAGCT TGTTAACCAT 
AAAAACATAA TTGGCTTACT TAATGCTTTT ACGCCGCAAA GGAACTTGGA AGAGTTTCAG 
GATGTCTACC TGGTCATGGA GCTGATGGAC GCTAATCTCT GCCAGGTCAT CCAAATGGAC 
TTGGATCACG ACAGAATGTC CTATTTGCTT TATCAAATGT TGTGCGGAAT AAAACATTTA 
CACTCAGCAG GAATTATTCA CAGAGACTTA AAGCCATCCA ATATAGTTGT AAAGGCCGAC 
TGCACTCTAA AAATTTTGGA TTTCGGTCTG GCACGTACTG CAGGAACTAC CTTTATGATG 
ACTCCCTATG TGGTCACCCG ATATTACAGG GCTCCAGAGG TCATTTTGGG CATGGGATAC 
ACAGAGAACG TGGACATATG GTCCGTCGGT TGCATTATGG GCGAAATGAT ACGGGGCGGT 
GTGCTGTTTC CTGGAACGGA TCATATAGAT CAGTGGAATA AAATTATTGA GCAATTAGGT 
ACACCATCAC CCTCGTTTAT GCAACGGTTA CAGCCAACCG TCCGAAACTA TGTGGAAAAT 
CGTCCTAGAT ACACTGGATA CTCATTTGAT AGACTTTTCC CCGATGGCTT GTTTCCAAAT 
GATAATAATC AGAATAGCCG CCGGAAGGCT TCTGACGCTA GAAATCTTCT TAGTAAAATG 
CTAGTCATTG ATCCAGAGCA GCGGATATCT GTGGACGAGG CTTTGAAGCA TGAATATATC 
AACGTGTGGT ATGATGCTGA GGAAGTGGAT GCTCCCGCTC CGGAGCCATA TGATCACAGC 
GTGGACGAAA GGGAACACAC TGTGGAGCAG TGGAAGGAGC TAATCTATGA GGAAGTCATG 
GACTACGAAG CACACAATAC AAATAATAGA ACGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bsk ( NM_001169459.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162930.1 Drosophila melanogaster basket (bsk), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa15210
Clone ID Related Accession (Same CDS sequence)	NM_164900.3 , NM_001169461.3 , NM_001169459.1
Accession Version	NM_001169459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	basket, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACAG CTCAGCACCA ACACTACACC GTCGAGGTGG GGGACACCAA CTTCACCATC 
CACAGTCGGT ACATTAATCT ACGGCCCATA GGATCAGGTG CCCAAGGAAT AGTATGCGCC 
GCTTACGATA CTATCACCCA GCAAAATGTG GCGATTAAGA AACTATCTCG ACCATTCCAA 
AATGTAACCC ATGCCAAGCG AGCATATAGG GAATTCAAGC TAATGAAGCT TGTTAACCAT 
AAAAACATAA TTGGCTTACT TAATGCTTTT ACGCCGCAAA GGAACTTGGA AGAGTTTCAG 
GATGTCTACC TGGTCATGGA GCTGATGGAC GCTAATCTCT GCCAGGTCAT CCAAATGGAC 
TTGGATCACG ACAGAATGTC CTATTTGCTT TATCAAATGT TGTGCGGAAT AAAACATTTA 
CACTCAGCAG GAATTATTCA CAGAGACTTA AAGCCATCCA ATATAGTTGT AAAGGCCGAC 
TGCACTCTAA AAATTTTGGA TTTCGGTCTG GCACGTACTG CAGGAACTAC CTTTATGATG 
ACTCCCTATG TGGTCACCCG ATATTACAGG GCTCCAGAGG TCATTTTGGG CATGGGATAC 
ACAGAGAACG TGGACATATG GTCCGTCGGT TGCATTATGG GCGAAATGAT ACGGGGCGGT 
GTGCTGTTTC CTGGAACGGA TCATATAGAT CAGTGGAATA AAATTATTGA GCAATTAGGT 
ACACCATCAC CCTCGTTTAT GCAACGGTTA CAGCCAACCG TCCGAAACTA TGTGGAAAAT 
CGTCCTAGAT ACACTGGATA CTCATTTGAT AGACTTTTCC CCGATGGCTT GTTTCCAAAT 
GATAATAATC AGAATAGCCG CCGGAAGGCT TCTGACGCTA GAAATCTTCT TAGTAAAATG 
CTAGTCATTG ATCCAGAGCA GCGGATATCT GTGGACGAGG CTTTGAAGCA TGAATATATC 
AACGTGTGGT ATGATGCTGA GGAAGTGGAT GCTCCCGCTC CGGAGCCATA TGATCACAGC 
GTGGACGAAA GGGAACACAC TGTGGAGCAG TGGAAGGAGC TAATCTATGA GGAAGTCATG 
GACTACGAAG CACACAATAC AAATAATAGA ACGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162930.1
CDS	533..1651
Translation	MTTAQHQHYTVEVGDTNFTIHSRYINLRPIGSGAQGIVCAAYDTITQQNVAIKKLSRPFQNVTHAKRAYREFKLMKLVNHKNIIGLLNAFTPQRNLEEFQDVYLVMELMDANLCQVIQMDLDHDRMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKADCTLKILDFGLARTAGTTFMMTPYVVTRYYRAPEVILGMGYTENVDIWSVGCIMGEMIRGGVLFPGTDHIDQWNKIIEQLGTPSPSFMQRLQPTVRNYVENRPRYTGYSFDRLFPDGLFPNDNNQNSRRKASDARNLLSKMLVIDPEQRISVDEALKHEYINVWYDAEEVDAPAPEPYDHSVDEREHTVEQWKELIYEEVMDYEAHNTNNRTR

Target ORF information:

RefSeq Version	NM_001169459.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster basket (bsk), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169459.1

ORF Insert Sequence:

ATGACGACAG CTCAGCACCA ACACTACACC GTCGAGGTGG GGGACACCAA CTTCACCATC 
CACAGTCGGT ACATTAATCT ACGGCCCATA GGATCAGGTG CCCAAGGAAT AGTATGCGCC 
GCTTACGATA CTATCACCCA GCAAAATGTG GCGATTAAGA AACTATCTCG ACCATTCCAA 
AATGTAACCC ATGCCAAGCG AGCATATAGG GAATTCAAGC TAATGAAGCT TGTTAACCAT 
AAAAACATAA TTGGCTTACT TAATGCTTTT ACGCCGCAAA GGAACTTGGA AGAGTTTCAG 
GATGTCTACC TGGTCATGGA GCTGATGGAC GCTAATCTCT GCCAGGTCAT CCAAATGGAC 
TTGGATCACG ACAGAATGTC CTATTTGCTT TATCAAATGT TGTGCGGAAT AAAACATTTA 
CACTCAGCAG GAATTATTCA CAGAGACTTA AAGCCATCCA ATATAGTTGT AAAGGCCGAC 
TGCACTCTAA AAATTTTGGA TTTCGGTCTG GCACGTACTG CAGGAACTAC CTTTATGATG 
ACTCCCTATG TGGTCACCCG ATATTACAGG GCTCCAGAGG TCATTTTGGG CATGGGATAC 
ACAGAGAACG TGGACATATG GTCCGTCGGT TGCATTATGG GCGAAATGAT ACGGGGCGGT 
GTGCTGTTTC CTGGAACGGA TCATATAGAT CAGTGGAATA AAATTATTGA GCAATTAGGT 
ACACCATCAC CCTCGTTTAT GCAACGGTTA CAGCCAACCG TCCGAAACTA TGTGGAAAAT 
CGTCCTAGAT ACACTGGATA CTCATTTGAT AGACTTTTCC CCGATGGCTT GTTTCCAAAT 
GATAATAATC AGAATAGCCG CCGGAAGGCT TCTGACGCTA GAAATCTTCT TAGTAAAATG 
CTAGTCATTG ATCCAGAGCA GCGGATATCT GTGGACGAGG CTTTGAAGCA TGAATATATC 
AACGTGTGGT ATGATGCTGA GGAAGTGGAT GCTCCCGCTC CGGAGCCATA TGATCACAGC 
GTGGACGAAA GGGAACACAC TGTGGAGCAG TGGAAGGAGC TAATCTATGA GGAAGTCATG 
GACTACGAAG CACACAATAC AAATAATAGA ACGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bsk cDNA ORF clone, Drosophila melanogaster(fruit fly)

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