Miro cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Miro
Entrez Gene ID	42845
Full Name	Mitochondrial Rho
Synonyms	B682,CG5410,DmMiro,Dmel\CG5410,Q8IMX7-1,Q8IMX7-2,dMiro,dmiro,miro
General protein information	Preferred Names Mitochondrial Rho Names CG5410 gene product from transcript CG5410-RF CG5410-PC CG5410-PD CG5410-PE CG5410-PF Miro-PC Miro-PD Miro-PE Miro-PF mitochondrial Rho mitochondrial Rho-GTPase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95D8-95D9

mRNA and Protein(s)

mRNA	Protein	Name
NM_170111.3	NP_732936.1	mitochondrial Rho, isoform D
NM_142948.4	NP_651205.2	mitochondrial Rho, isoform E
NM_001275966.1	NP_001262895.1	mitochondrial Rho, isoform F
NM_170114.3	NP_732939.2	mitochondrial Rho, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	RHOT1	NP_001028738.1
Mus musculus (house mouse)	Rhot1	NP_001156827.1
Danio rerio (zebrafish)	rhot1a	NP_997869.1
Caenorhabditis elegans (roundworm)	miro-1	NP_500620.1
Gallus gallus (chicken)	RHOT1	NP_001006208.1
Danio rerio (zebrafish)	LOC100535268	XP_003198159.1
Arabidopsis thaliana (thale cress)	MIRO2	NP_567139.1
Pan troglodytes (chimpanzee)	RHOT1	XP_511399.3
	LOC717901	XP_002800404.1
Xenopus tropicalis (tropical clawed frog)	LOC100492251	XP_004918458.1
Bos taurus (cattle)	RHOT1	NP_001039547.1
Canis lupus familiaris (dog)	RHOT1	XP_003435351.1
Rattus norvegicus (Norway rat)	Rhot1	NP_001100496.1
Drosophila melanogaster (fruit fly)	Miro	NP_732936.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007998	XP_317471.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A new mode of mitochondrial sorting in polarized cell growth, whereby bi-directional mitochondrial transport undertaken exclusively by dynein is regulated by Milton in the anterograde direction and by a Miro-dependent switch to the retrograde direction.
Title: A new mode of mitochondrial transport and polarized sorting regulated by Dynein, Milton and Miro.

we found that mitochondrial fission played a functional role in high-calcium induced necrosis, and a LOF vimar mutant rescued the mitochondrial fission defect and cell death.
Title: Vimar Is a Novel Regulator of Mitochondrial Fission through Miro.

Both conditions impaired NSC lineage progression. Ca(2) mito homeostasis is influenced by Polo-mediated phosphorylation of a conserved residue in Miro
Title: Polo Kinase Phosphorylates Miro to Control ER-Mitochondria Contact Sites and Mitochondrial Ca(2+) Homeostasis in Neural Stem Cell Development.

The results of this study showed that dMiro's N-terminal GTPase domain is critically required for viability, mitochondrial size, and the distribution of mitochondria out of the neuronal soma.
Title: Miro's N-terminal GTPase domain is required for transport of mitochondria into axons and dendrites.

PINK1-mediated phosphorylation of Miro inhibits synaptic growth and protects dopaminergic neurons in Drosophila.
Title: PINK1-mediated phosphorylation of Miro inhibits synaptic growth and protects dopaminergic neurons in Drosophila.

The following Miro gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Miro cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05879	NM_170111.3 Latest version!	Drosophila melanogaster mitochondrial Rho, transcript variant D (Miro), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05880	NM_170114.3 Latest version!	Drosophila melanogaster mitochondrial Rho, transcript variant C (Miro), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05881	NM_142948.4 Latest version!	Drosophila melanogaster mitochondrial Rho, transcript variant E (Miro), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa05881	NM_001275966.1 Latest version!	Drosophila melanogaster mitochondrial Rho, transcript variant F (Miro), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Miro ( NM_170111.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732936.1 Drosophila melanogaster mitochondrial Rho, transcript variant D (Miro), mRNA.

Price & Availability↑

CloneID	OFa05879
Clone ID Related Accession (Same CDS sequence)	NM_170111.3
Accession Version	NM_170111.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial Rho, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170111.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGACAGT ACACGGCGTC GCAGCGCAAG AATGTTAGGA TCCTGCTCGT CGGCGACGCC 
GGGGTGGGTA AGACGTCGTT GATTCTGTCT CTGGTCAGTG AGGAGTACCC GGAGGAGGTT 
CCGCCTAGGG CTGAGGAGAT TACCATTCCG GCGAACGTGA CGCCCGAGCA GGTGCCCACC 
AGCATAGTTG ACTTCTCGGC CGTGGAGCAG TCGGAGGATG CCCTGGCCGC CGAAATTAAC 
AAGGCGCACG TGGTGTGCAT AGTGTACGCC GTGGACGATG ACGACACTTT GGATCGGATC 
ACATCCCATT GGCTGCCGCT CGTCCGGGCC AAGTGCAATC CCTCCCTGGA CGGCGAGGGT 
GATGCCGAAG CGGAGGCGGA AGGAGACACA CAACGAGAGC CCATTCGGAA GCCAATCGTT 
CTGGTGGGTA ACAAAATAGA CCTCATCGAG TATTCCACCA TGGACAGCGT GCTGGCCATC 
ATGGAAGACT ACCCCGAGAT TGAGAGTTGC GTGGAGTGCT CTGCCAAGTC GCTGCACAAC 
ATCTCCGAGA TGTTTTACTA CGCCCAGAAG GCTGTGCTGC ACCCGACTTC TCCCCTGTAT 
ATGATGGAAG AACAGGAGCT CACATCCGCC TGCAAGAAGT CGCTGGTGCG CATTTTCAAG 
ATCTGCGACA TTGACGGGGA CAATCTGTTG AACGATTACG AGCTGAATTT ATTCCAGCGA 
CGCTGTTTCA ACACACCACT CCAGCCGCAA ATCCTTGATG AGGTGAAGGC CGTGATACAG 
AAAAATGTGC CCGATGGCAT ATACAACGAT GCGGTCACCC TGAAGGGCTT CCTCTTCTTA 
CACTGCCTTT TCATTCAGCG GGGAAGGAAC GAGACAACCT GGGCGGTGTT ACGGCGCTTT 
GGCTATAACG ACCAGTTGGA GATGTGCCAG GAGTATCTTA GGCCACCGCT GAAAATACCG 
CCTGGCAGCA GCACAGAACT CTCGCACCGC GGACAGCAGT TTCTGATTGC CGTGTTTGAG 
CGCTATGATC GCGATGGCGA CGGAGCCTTA TCACCTGAGG AGCACAAGAT GCTCTTCAGC 
ACATGCCCGG CTGCTCCGTG GTCCTACTCC ACCGACATAC GCAAGTCCTG CCCGATCAAC 
GAGACAACTG GATGGGTGAC ACTGCACGGG TGGCTATGTC GGTGGACACT GATGACGCTG 
ATTGATGTGG TCAAAACAAT GGAGTATTTG GCCTATTTGG GCTTCAATGT TCATGAAAAC 
GACAGCCAGT TGGCGGCAAT TCACGTAACT CGAGAGCGCC GCATCGATTT GGCCAAGCGC 
CAAAGCAGTC GGTCCGTTTA CAAGTGTCAT GTGATTGGAC CAAAGGGATC AGGAAAGACT 
GGAATGTGCA GGGGATTCCT GGTGGAGGAT ATGCACAAAC TAATCGGAAA AGAGTTTAAA 
ACGAATGTGG TTAATTGCAT CAACTCTGTG CAGGTGTATG GCCAGGAAAA GCACCTCATT 
CTGCGTGACA TAGACGTCAG GCATGCCCTC GATCCCCTCC AGCCACAAGA AGTCAATTGC 
GATGTTGCCT GCCTAGTCTA CGACTCATCT AATCCCCGTT CCTTTGAGTA CGTGGCCCGC 
ATCTACATCA AGTATTATGC GGAGAGCAAG ATTCCAGTGA TGATAGTCGG CACCAAGTGC 
GACATGGACG AACGCCGGCA GGACTATCTA ATGCAGCCCT CTGAATTCTG TGACAAGTAC 
AAGTTGCTGC CTCCGCATCT CTTCAGCCTG AAAACCAACA AAAAAGAACT GTATACCAAG 
CTGGCCACAA TGGCAGCGTT TCCTCACCTG AGGCAATTCG GCCTGATGAC GGAGGACCCC 
AAGCTGTGGT TAAAGGCGGG ACTAGGAGTG GCCGCAGCTA CCATGCTGGG ATTTATAGTG 
CTGAAAACGA TAAGTGCTGC CGGAGCCCAC ACCCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732936.1
CDS	183..2141
Translation	MGQYTASQRKNVRILLVGDAGVGKTSLILSLVSEEYPEEVPPRAEEITIPANVTPEQVPTSIVDFSAVEQSEDALAAEINKAHVVCIVYAVDDDDTLDRITSHWLPLVRAKCNPSLDGEGDAEAEAEGDTQREPIRKPIVLVGNKIDLIEYSTMDSVLAIMEDYPEIESCVECSAKSLHNISEMFYYAQKAVLHPTSPLYMMEEQELTSACKKSLVRIFKICDIDGDNLLNDYELNLFQRRCFNTPLQPQILDEVKAVIQKNVPDGIYNDAVTLKGFLFLHCLFIQRGRNETTWAVLRRFGYNDQLEMCQEYLRPPLKIPPGSSTELSHRGQQFLIAVFERYDRDGDGALSPEEHKMLFSTCPAAPWSYSTDIRKSCPINETTGWVTLHGWLCRWTLMTLIDVVKTMEYLAYLGFNVHENDSQLAAIHVTRERRIDLAKRQSSRSVYKCHVIGPKGSGKTGMCRGFLVEDMHKLIGKEFKTNVVNCINSVQVYGQEKHLILRDIDVRHALDPLQPQEVNCDVACLVYDSSNPRSFEYVARIYIKYYAESKIPVMIVGTKCDMDERRQDYLMQPSEFCDKYKLLPPHLFSLKTNKKELYTKLATMAAFPHLRQFGLMTEDPKLWLKAGLGVAAATMLGFIVLKTISAAGAHTR

Target ORF information:

RefSeq Version	NM_170111.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial Rho, transcript variant D (Miro), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170111.3

ORF Insert Sequence:

ATGGGACAGT ACACGGCGTC GCAGCGCAAG AATGTTAGGA TCCTGCTCGT CGGCGACGCC 
GGGGTGGGTA AGACGTCGTT GATTCTGTCT CTGGTCAGTG AGGAGTACCC GGAGGAGGTT 
CCGCCTAGGG CTGAGGAGAT TACCATTCCG GCGAACGTGA CGCCCGAGCA GGTGCCCACC 
AGCATAGTTG ACTTCTCGGC CGTGGAGCAG TCGGAGGATG CCCTGGCCGC CGAAATTAAC 
AAGGCGCACG TGGTGTGCAT AGTGTACGCC GTGGACGATG ACGACACTTT GGATCGGATC 
ACATCCCATT GGCTGCCGCT CGTCCGGGCC AAGTGCAATC CCTCCCTGGA CGGCGAGGGT 
GATGCCGAAG CGGAGGCGGA AGGAGACACA CAACGAGAGC CCATTCGGAA GCCAATCGTT 
CTGGTGGGTA ACAAAATAGA CCTCATCGAG TATTCCACCA TGGACAGCGT GCTGGCCATC 
ATGGAAGACT ACCCCGAGAT TGAGAGTTGC GTGGAGTGCT CTGCCAAGTC GCTGCACAAC 
ATCTCCGAGA TGTTTTACTA CGCCCAGAAG GCTGTGCTGC ACCCGACTTC TCCCCTGTAT 
ATGATGGAAG AACAGGAGCT CACATCCGCC TGCAAGAAGT CGCTGGTGCG CATTTTCAAG 
ATCTGCGACA TTGACGGGGA CAATCTGTTG AACGATTACG AGCTGAATTT ATTCCAGCGA 
CGCTGTTTCA ACACACCACT CCAGCCGCAA ATCCTTGATG AGGTGAAGGC CGTGATACAG 
AAAAATGTGC CCGATGGCAT ATACAACGAT GCGGTCACCC TGAAGGGCTT CCTCTTCTTA 
CACTGCCTTT TCATTCAGCG GGGAAGGAAC GAGACAACCT GGGCGGTGTT ACGGCGCTTT 
GGCTATAACG ACCAGTTGGA GATGTGCCAG GAGTATCTTA GGCCACCGCT GAAAATACCG 
CCTGGCAGCA GCACAGAACT CTCGCACCGC GGACAGCAGT TTCTGATTGC CGTGTTTGAG 
CGCTATGATC GCGATGGCGA CGGAGCCTTA TCACCTGAGG AGCACAAGAT GCTCTTCAGC 
ACATGCCCGG CTGCTCCGTG GTCCTACTCC ACCGACATAC GCAAGTCCTG CCCGATCAAC 
GAGACAACTG GATGGGTGAC ACTGCACGGG TGGCTATGTC GGTGGACACT GATGACGCTG 
ATTGATGTGG TCAAAACAAT GGAGTATTTG GCCTATTTGG GCTTCAATGT TCATGAAAAC 
GACAGCCAGT TGGCGGCAAT TCACGTAACT CGAGAGCGCC GCATCGATTT GGCCAAGCGC 
CAAAGCAGTC GGTCCGTTTA CAAGTGTCAT GTGATTGGAC CAAAGGGATC AGGAAAGACT 
GGAATGTGCA GGGGATTCCT GGTGGAGGAT ATGCACAAAC TAATCGGAAA AGAGTTTAAA 
ACGAATGTGG TTAATTGCAT CAACTCTGTG CAGGTGTATG GCCAGGAAAA GCACCTCATT 
CTGCGTGACA TAGACGTCAG GCATGCCCTC GATCCCCTCC AGCCACAAGA AGTCAATTGC 
GATGTTGCCT GCCTAGTCTA CGACTCATCT AATCCCCGTT CCTTTGAGTA CGTGGCCCGC 
ATCTACATCA AGTATTATGC GGAGAGCAAG ATTCCAGTGA TGATAGTCGG CACCAAGTGC 
GACATGGACG AACGCCGGCA GGACTATCTA ATGCAGCCCT CTGAATTCTG TGACAAGTAC 
AAGTTGCTGC CTCCGCATCT CTTCAGCCTG AAAACCAACA AAAAAGAACT GTATACCAAG 
CTGGCCACAA TGGCAGCGTT TCCTCACCTG AGGCAATTCG GCCTGATGAC GGAGGACCCC 
AAGCTGTGGT TAAAGGCGGG ACTAGGAGTG GCCGCAGCTA CCATGCTGGG ATTTATAGTG 
CTGAAAACGA TAAGTGCTGC CGGAGCCCAC ACCCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Miro ( NM_170114.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732939.2 Drosophila melanogaster mitochondrial Rho, transcript variant C (Miro), mRNA.

Price & Availability↑

CloneID	OFa05880
Clone ID Related Accession (Same CDS sequence)	NM_170114.3
Accession Version	NM_170114.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial Rho, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170114.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGACAGT ACACGGCGTC GCAGCGCAAG AATGTTAGGA TCCTGCTCGT CGGCGACGCC 
GGGGTGGGTA AGACGTCGTT GATTCTGTCT CTGGTCAGTG AGGAGTACCC GGAGGAGGTT 
CCGCCTAGGG CTGAGGAGAT TACCATTCCG GCGAACGTGA CGCCCGAGCA GGTGCCCACC 
AGCATAGTTG ACTTCTCGGC CGTGGAGCAG TCGGAGGATG CCCTGGCCGC CGAAATTAAC 
AAGGCGCACG TGGTGTGCAT AGTGTACGCC GTGGACGATG ACGACACTTT GGATCGGATC 
ACATCCCATT GGCTGCCGCT CGTCCGGGCC AAGTGCAATC CCTCCCTGGA CGGCGAGGGT 
GATGCCGAAG CGGAGGCGGA AGGAGACACA CAACGAGAGC CCATTCGGAA GCCAATCGTT 
CTGGTGGGTA ACAAAATAGA CCTCATCGAG TATTCCACCA TGGACAGCGT GCTGGCCATC 
ATGGAAGACT ACCCCGAGAT TGAGAGTTGC GTGGAGTGCT CTGCCAAGTC GCTGCACAAC 
ATCTCCGAGA TGTTTTACTA CGCCCAGAAG GCTGTGCTGC ACCCGACTTC TCCCCTGTAT 
ATGATGGAAG AACAGGAGCT CACATCCGCC TGCAAGAAGT CGCTGGTGCG CATTTTCAAG 
ATCTGCGACA TTGACGGGGA CAATCTGTTG AACGATTACG AGCTGAATTT ATTCCAGCGA 
CGCTGTTTCA ACACACCACT CCAGCCGCAA ATCCTTGATG AGGTGAAGGC CGTGATACAG 
AAAAATGTGC CCGATGGCAT ATACAACGAT GCGGTCACCC TGAAGGGCTT CCTCTTCTTA 
CACTGCCTTT TCATTCAGCG GGGAAGGAAC GAGACAACCT GGGCGGTGTT ACGGCGCTTT 
GGCTATAACG ACCAGTTGGA GATGTGCCAG GAGTATCTTA GGCCACCGCT GAAAATACCG 
CCTGGCAGCA GCACAGAACT CTCGCACCGC GGACAGCAGT TTCTGATTGC CGTGTTTGAG 
CGCTATGATC GCGATGGCGA CGGAGCCTTA TCACCTGAGG AGCACAAGAT GCTCTTCAGC 
ACATGCCCGG CTGCTCCGTG GTCCTACTCC ACCGACATAC GCAAGTCCTG CCCGATCAAC 
GAGACAACTG GATGGGTGAC ACTGCACGGG TGGCTATGTC GGTGGACACT GATGACGCTG 
ATTGATGTGG TCAAAACAAT GGAGTATTTG GCCTATTTGG GCTTCAATGT TCATGAAAAC 
GACAGCCAGT TGGCGGCAAT TCACGTAACT CGAGAGCGCC GCATCGATTT GGCCAAGCGC 
CAAAGCAGTC GGTCCGTTTA CAAGTGTCAT GTGATTGGAC CAAAGGGATC AGGAAAGACT 
GGAATGTGCA GGGGATTCCT GGTGGAGGAT ATGCACAAAC TAATCGGAAA AGAGTTTAAA 
ACGAATGTGG TTAATTGCAT CAACTCTGTG CAGGTGTATG GCCAGGAAAA GCACCTCATT 
CTGCGTGACA TAGACGTCAG GCATGCCCTC GATCCCCTCC AGCCACAAGA AGTCAATTGC 
GATGTTGCCT GCCTAGTCTA CGACTCATCT AATCCCCGTT CCTTTGAGTA CGTGGCCCGC 
ATCTACATCA AGTATTATGC GGAGAGCAAG ATTCCAGTGA TGATAGTCGG CACCAAGTGC 
GACATGGACG AACGCCGGCA GGACTATCTA ATGCAGCCCT CTGAATTCTG TGACAAGTAC 
AAGTTGCTGC CTCCGCATCT CTTCAGCCTG AAAACCAACA AAAAAGAACT GTATACCAAG 
CTGGCCACAA TGGCAGCGTT TCCTCGCTTC CAGGCCGCCT GGATACTGTT CTACAAGCAC 
AGTCACCTGA GGCAATTCGG CCTGATGACG GAGGACCCCA AGCTGTGGTT AAAGGCGGGA 
CTAGGAGTGG CCGCAGCTAC CATGCTGGGA TTTATAGTGC TGAAAACGAT AAGTGCTGCC 
GGAGCCCACA CCCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732939.2
CDS	183..2180
Translation	MGQYTASQRKNVRILLVGDAGVGKTSLILSLVSEEYPEEVPPRAEEITIPANVTPEQVPTSIVDFSAVEQSEDALAAEINKAHVVCIVYAVDDDDTLDRITSHWLPLVRAKCNPSLDGEGDAEAEAEGDTQREPIRKPIVLVGNKIDLIEYSTMDSVLAIMEDYPEIESCVECSAKSLHNISEMFYYAQKAVLHPTSPLYMMEEQELTSACKKSLVRIFKICDIDGDNLLNDYELNLFQRRCFNTPLQPQILDEVKAVIQKNVPDGIYNDAVTLKGFLFLHCLFIQRGRNETTWAVLRRFGYNDQLEMCQEYLRPPLKIPPGSSTELSHRGQQFLIAVFERYDRDGDGALSPEEHKMLFSTCPAAPWSYSTDIRKSCPINETTGWVTLHGWLCRWTLMTLIDVVKTMEYLAYLGFNVHENDSQLAAIHVTRERRIDLAKRQSSRSVYKCHVIGPKGSGKTGMCRGFLVEDMHKLIGKEFKTNVVNCINSVQVYGQEKHLILRDIDVRHALDPLQPQEVNCDVACLVYDSSNPRSFEYVARIYIKYYAESKIPVMIVGTKCDMDERRQDYLMQPSEFCDKYKLLPPHLFSLKTNKKELYTKLATMAAFPRFQAAWILFYKHSHLRQFGLMTEDPKLWLKAGLGVAAATMLGFIVLKTISAAGAHTR

Target ORF information:

RefSeq Version	NM_170114.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial Rho, transcript variant C (Miro), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170114.3

ORF Insert Sequence:

ATGGGACAGT ACACGGCGTC GCAGCGCAAG AATGTTAGGA TCCTGCTCGT CGGCGACGCC 
GGGGTGGGTA AGACGTCGTT GATTCTGTCT CTGGTCAGTG AGGAGTACCC GGAGGAGGTT 
CCGCCTAGGG CTGAGGAGAT TACCATTCCG GCGAACGTGA CGCCCGAGCA GGTGCCCACC 
AGCATAGTTG ACTTCTCGGC CGTGGAGCAG TCGGAGGATG CCCTGGCCGC CGAAATTAAC 
AAGGCGCACG TGGTGTGCAT AGTGTACGCC GTGGACGATG ACGACACTTT GGATCGGATC 
ACATCCCATT GGCTGCCGCT CGTCCGGGCC AAGTGCAATC CCTCCCTGGA CGGCGAGGGT 
GATGCCGAAG CGGAGGCGGA AGGAGACACA CAACGAGAGC CCATTCGGAA GCCAATCGTT 
CTGGTGGGTA ACAAAATAGA CCTCATCGAG TATTCCACCA TGGACAGCGT GCTGGCCATC 
ATGGAAGACT ACCCCGAGAT TGAGAGTTGC GTGGAGTGCT CTGCCAAGTC GCTGCACAAC 
ATCTCCGAGA TGTTTTACTA CGCCCAGAAG GCTGTGCTGC ACCCGACTTC TCCCCTGTAT 
ATGATGGAAG AACAGGAGCT CACATCCGCC TGCAAGAAGT CGCTGGTGCG CATTTTCAAG 
ATCTGCGACA TTGACGGGGA CAATCTGTTG AACGATTACG AGCTGAATTT ATTCCAGCGA 
CGCTGTTTCA ACACACCACT CCAGCCGCAA ATCCTTGATG AGGTGAAGGC CGTGATACAG 
AAAAATGTGC CCGATGGCAT ATACAACGAT GCGGTCACCC TGAAGGGCTT CCTCTTCTTA 
CACTGCCTTT TCATTCAGCG GGGAAGGAAC GAGACAACCT GGGCGGTGTT ACGGCGCTTT 
GGCTATAACG ACCAGTTGGA GATGTGCCAG GAGTATCTTA GGCCACCGCT GAAAATACCG 
CCTGGCAGCA GCACAGAACT CTCGCACCGC GGACAGCAGT TTCTGATTGC CGTGTTTGAG 
CGCTATGATC GCGATGGCGA CGGAGCCTTA TCACCTGAGG AGCACAAGAT GCTCTTCAGC 
ACATGCCCGG CTGCTCCGTG GTCCTACTCC ACCGACATAC GCAAGTCCTG CCCGATCAAC 
GAGACAACTG GATGGGTGAC ACTGCACGGG TGGCTATGTC GGTGGACACT GATGACGCTG 
ATTGATGTGG TCAAAACAAT GGAGTATTTG GCCTATTTGG GCTTCAATGT TCATGAAAAC 
GACAGCCAGT TGGCGGCAAT TCACGTAACT CGAGAGCGCC GCATCGATTT GGCCAAGCGC 
CAAAGCAGTC GGTCCGTTTA CAAGTGTCAT GTGATTGGAC CAAAGGGATC AGGAAAGACT 
GGAATGTGCA GGGGATTCCT GGTGGAGGAT ATGCACAAAC TAATCGGAAA AGAGTTTAAA 
ACGAATGTGG TTAATTGCAT CAACTCTGTG CAGGTGTATG GCCAGGAAAA GCACCTCATT 
CTGCGTGACA TAGACGTCAG GCATGCCCTC GATCCCCTCC AGCCACAAGA AGTCAATTGC 
GATGTTGCCT GCCTAGTCTA CGACTCATCT AATCCCCGTT CCTTTGAGTA CGTGGCCCGC 
ATCTACATCA AGTATTATGC GGAGAGCAAG ATTCCAGTGA TGATAGTCGG CACCAAGTGC 
GACATGGACG AACGCCGGCA GGACTATCTA ATGCAGCCCT CTGAATTCTG TGACAAGTAC 
AAGTTGCTGC CTCCGCATCT CTTCAGCCTG AAAACCAACA AAAAAGAACT GTATACCAAG 
CTGGCCACAA TGGCAGCGTT TCCTCGCTTC CAGGCCGCCT GGATACTGTT CTACAAGCAC 
AGTCACCTGA GGCAATTCGG CCTGATGACG GAGGACCCCA AGCTGTGGTT AAAGGCGGGA 
CTAGGAGTGG CCGCAGCTAC CATGCTGGGA TTTATAGTGC TGAAAACGAT AAGTGCTGCC 
GGAGCCCACA CCCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Miro ( NM_142948.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651205.2 Drosophila melanogaster mitochondrial Rho, transcript variant E (Miro), mRNA.

Price & Availability↑

CloneID	OFa05881
Clone ID Related Accession (Same CDS sequence)	NM_142948.4 , NM_001275966.1
Accession Version	NM_142948.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial Rho, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142948.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGACAGT ACACGGCGTC GCAGCGCAAG AATGTTAGGA TCCTGCTCGT CGGCGACGCC 
GGGGTGGGTA AGACGTCGTT GATTCTGTCT CTGGTCAGTG AGGAGTACCC GGAGGAGGTT 
CCGCCTAGGG CTGAGGAGAT TACCATTCCG GCGAACGTGA CGCCCGAGCA GGTGCCCACC 
AGCATAGTTG ACTTCTCGGC CGTGGAGCAG TCGGAGGATG CCCTGGCCGC CGAAATTAAC 
AAGGCGCACG TGGTGTGCAT AGTGTACGCC GTGGACGATG ACGACACTTT GGATCGGATC 
ACATCCCATT GGCTGCCGCT CGTCCGGGCC AAGTGCAATC CCTCCCTGGA CGGCGAGGGT 
GATGCCGAAG CGGAGGCGGA AGGAGACACA CAACGAGAGC CCATTCGGAA GCCAATCGTT 
CTGGTGGGTA ACAAAATAGA CCTCATCGAG TATTCCACCA TGGACAGCGT GCTGGCCATC 
ATGGAAGACT ACCCCGAGAT TGAGAGTTGC GTGGAGTGCT CTGCCAAGTC GCTGCACAAC 
ATCTCCGAGA TGTTTTACTA CGCCCAGAAG GCTGTGCTGC ACCCGACTTC TCCCCTGTAT 
ATGATGGAAG AACAGGAGCT CACATCCGCC TGCAAGAAGT CGCTGGTGCG CATTTTCAAG 
ATCTGCGACA TTGACGGGGA CAATCTGTTG AACGATTACG AGCTGAATTT ATTCCAGCGA 
CGCTGTTTCA ACACACCACT CCAGCCGCAA ATCCTTGATG AGGTGAAGGC CGTGATACAG 
AAAAATGTGC CCGATGGCAT ATACAACGAT GCGGTCACCC TGAAGGGCTT CCTCTTCTTA 
CACTGCCTTT TCATTCAGCG GGGAAGGAAC GAGACAACCT GGGCGGTGTT ACGGCGCTTT 
GGCTATAACG ACCAGTTGGA GATGTGCCAG GAGTATCTTA GGCCACCGCT GAAAATACCG 
CCTGGCAGCA GCACAGAACT CTCGCACCGC GGACAGCAGT TTCTGATTGC CGTGTTTGAG 
CGCTATGATC GCGATGGCGA CGGAGCCTTA TCACCTGAGG AGCACAAGAT GCTCTTCAGC 
ACATGCCCGG CTGCTCCGTG GTCCTACTCC ACCGACATAC GCAAGTCCTG CCCGATCAAC 
GAGACAACTG GATGGGTGAC ACTGCACGGG TGGCTATGTC GGTGGACACT GATGACGCTG 
ATTGATGTGG TCAAAACAAT GGAGTATTTG GCCTATTTGG GCTTCAATGT TCATGAAAAC 
GACAGCCAGT TGGCGGCAAT TCACGTAACT CGAGAGCGCC GCATCGATTT GGCCAAGCGC 
CAAAGCAGTC GGTCCGTTTA CAAGTGTCAT GTGATTGGAC CAAAGGGATC AGGAAAGACT 
GGAATGTGCA GGGGATTCCT GGTGGAGGAT ATGCACAAAC TAATCGGAAA AGAGTTTAAA 
ACGAATGTGG TTAATTGCAT CAACTCTGTG CAGGTGTATG GCCAGGAAAA GCACCTCATT 
CTGCGTGACA TAGACGTCAG GCATGCCCTC GATCCCCTCC AGCCACAAGA AGTCAATTGC 
GATGTTGCCT GCCTAGTCTA CGACTCATCT AATCCCCGTT CCTTTGAGTA CGTGGCCCGC 
ATCTACATCA AGTATTATGC GGAGAGCAAG ATTCCAGTGA TGATAGTCGG CACCAAGTGC 
GACATGGACG AACGCCGGCA GGACTATCTA ATGCAGCCCT CTGAATTCTG TGACAAGTAC 
AAGTTGCTGC CTCCGCATCT CTTCAGCCTG AAAACCAACA AAAAAGAACT GTATACCAAG 
CTGGCCACAA TGGCAGCGTT TCCTCGCTTC CAGGCCGCCT GGATACTGTT CTACAAGCAC 
AGGTTGGTAC AGCTGTGGGA GTCGGCTCAC CTGAGGCAAT TCGGCCTGAT GACGGAGGAC 
CCCAAGCTGT GGTTAAAGGC GGGACTAGGA GTGGCCGCAG CTACCATGCT GGGATTTATA 
GTGCTGAAAA CGATAAGTGC TGCCGGAGCC CACACCCGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651205.2
CDS	183..2204
Translation	MGQYTASQRKNVRILLVGDAGVGKTSLILSLVSEEYPEEVPPRAEEITIPANVTPEQVPTSIVDFSAVEQSEDALAAEINKAHVVCIVYAVDDDDTLDRITSHWLPLVRAKCNPSLDGEGDAEAEAEGDTQREPIRKPIVLVGNKIDLIEYSTMDSVLAIMEDYPEIESCVECSAKSLHNISEMFYYAQKAVLHPTSPLYMMEEQELTSACKKSLVRIFKICDIDGDNLLNDYELNLFQRRCFNTPLQPQILDEVKAVIQKNVPDGIYNDAVTLKGFLFLHCLFIQRGRNETTWAVLRRFGYNDQLEMCQEYLRPPLKIPPGSSTELSHRGQQFLIAVFERYDRDGDGALSPEEHKMLFSTCPAAPWSYSTDIRKSCPINETTGWVTLHGWLCRWTLMTLIDVVKTMEYLAYLGFNVHENDSQLAAIHVTRERRIDLAKRQSSRSVYKCHVIGPKGSGKTGMCRGFLVEDMHKLIGKEFKTNVVNCINSVQVYGQEKHLILRDIDVRHALDPLQPQEVNCDVACLVYDSSNPRSFEYVARIYIKYYAESKIPVMIVGTKCDMDERRQDYLMQPSEFCDKYKLLPPHLFSLKTNKKELYTKLATMAAFPRFQAAWILFYKHRLVQLWESAHLRQFGLMTEDPKLWLKAGLGVAAATMLGFIVLKTISAAGAHTR

Target ORF information:

RefSeq Version	NM_142948.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial Rho, transcript variant E (Miro), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142948.4

ORF Insert Sequence:

ATGGGACAGT ACACGGCGTC GCAGCGCAAG AATGTTAGGA TCCTGCTCGT CGGCGACGCC 
GGGGTGGGTA AGACGTCGTT GATTCTGTCT CTGGTCAGTG AGGAGTACCC GGAGGAGGTT 
CCGCCTAGGG CTGAGGAGAT TACCATTCCG GCGAACGTGA CGCCCGAGCA GGTGCCCACC 
AGCATAGTTG ACTTCTCGGC CGTGGAGCAG TCGGAGGATG CCCTGGCCGC CGAAATTAAC 
AAGGCGCACG TGGTGTGCAT AGTGTACGCC GTGGACGATG ACGACACTTT GGATCGGATC 
ACATCCCATT GGCTGCCGCT CGTCCGGGCC AAGTGCAATC CCTCCCTGGA CGGCGAGGGT 
GATGCCGAAG CGGAGGCGGA AGGAGACACA CAACGAGAGC CCATTCGGAA GCCAATCGTT 
CTGGTGGGTA ACAAAATAGA CCTCATCGAG TATTCCACCA TGGACAGCGT GCTGGCCATC 
ATGGAAGACT ACCCCGAGAT TGAGAGTTGC GTGGAGTGCT CTGCCAAGTC GCTGCACAAC 
ATCTCCGAGA TGTTTTACTA CGCCCAGAAG GCTGTGCTGC ACCCGACTTC TCCCCTGTAT 
ATGATGGAAG AACAGGAGCT CACATCCGCC TGCAAGAAGT CGCTGGTGCG CATTTTCAAG 
ATCTGCGACA TTGACGGGGA CAATCTGTTG AACGATTACG AGCTGAATTT ATTCCAGCGA 
CGCTGTTTCA ACACACCACT CCAGCCGCAA ATCCTTGATG AGGTGAAGGC CGTGATACAG 
AAAAATGTGC CCGATGGCAT ATACAACGAT GCGGTCACCC TGAAGGGCTT CCTCTTCTTA 
CACTGCCTTT TCATTCAGCG GGGAAGGAAC GAGACAACCT GGGCGGTGTT ACGGCGCTTT 
GGCTATAACG ACCAGTTGGA GATGTGCCAG GAGTATCTTA GGCCACCGCT GAAAATACCG 
CCTGGCAGCA GCACAGAACT CTCGCACCGC GGACAGCAGT TTCTGATTGC CGTGTTTGAG 
CGCTATGATC GCGATGGCGA CGGAGCCTTA TCACCTGAGG AGCACAAGAT GCTCTTCAGC 
ACATGCCCGG CTGCTCCGTG GTCCTACTCC ACCGACATAC GCAAGTCCTG CCCGATCAAC 
GAGACAACTG GATGGGTGAC ACTGCACGGG TGGCTATGTC GGTGGACACT GATGACGCTG 
ATTGATGTGG TCAAAACAAT GGAGTATTTG GCCTATTTGG GCTTCAATGT TCATGAAAAC 
GACAGCCAGT TGGCGGCAAT TCACGTAACT CGAGAGCGCC GCATCGATTT GGCCAAGCGC 
CAAAGCAGTC GGTCCGTTTA CAAGTGTCAT GTGATTGGAC CAAAGGGATC AGGAAAGACT 
GGAATGTGCA GGGGATTCCT GGTGGAGGAT ATGCACAAAC TAATCGGAAA AGAGTTTAAA 
ACGAATGTGG TTAATTGCAT CAACTCTGTG CAGGTGTATG GCCAGGAAAA GCACCTCATT 
CTGCGTGACA TAGACGTCAG GCATGCCCTC GATCCCCTCC AGCCACAAGA AGTCAATTGC 
GATGTTGCCT GCCTAGTCTA CGACTCATCT AATCCCCGTT CCTTTGAGTA CGTGGCCCGC 
ATCTACATCA AGTATTATGC GGAGAGCAAG ATTCCAGTGA TGATAGTCGG CACCAAGTGC 
GACATGGACG AACGCCGGCA GGACTATCTA ATGCAGCCCT CTGAATTCTG TGACAAGTAC 
AAGTTGCTGC CTCCGCATCT CTTCAGCCTG AAAACCAACA AAAAAGAACT GTATACCAAG 
CTGGCCACAA TGGCAGCGTT TCCTCGCTTC CAGGCCGCCT GGATACTGTT CTACAAGCAC 
AGGTTGGTAC AGCTGTGGGA GTCGGCTCAC CTGAGGCAAT TCGGCCTGAT GACGGAGGAC 
CCCAAGCTGT GGTTAAAGGC GGGACTAGGA GTGGCCGCAG CTACCATGCT GGGATTTATA 
GTGCTGAAAA CGATAAGTGC TGCCGGAGCC CACACCCGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Miro ( NM_001275966.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262895.1 Drosophila melanogaster mitochondrial Rho, transcript variant F (Miro), mRNA.

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CloneID	OFa05881
Clone ID Related Accession (Same CDS sequence)	NM_142948.4 , NM_001275966.1
Accession Version	NM_001275966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial Rho, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGACAGT ACACGGCGTC GCAGCGCAAG AATGTTAGGA TCCTGCTCGT CGGCGACGCC 
GGGGTGGGTA AGACGTCGTT GATTCTGTCT CTGGTCAGTG AGGAGTACCC GGAGGAGGTT 
CCGCCTAGGG CTGAGGAGAT TACCATTCCG GCGAACGTGA CGCCCGAGCA GGTGCCCACC 
AGCATAGTTG ACTTCTCGGC CGTGGAGCAG TCGGAGGATG CCCTGGCCGC CGAAATTAAC 
AAGGCGCACG TGGTGTGCAT AGTGTACGCC GTGGACGATG ACGACACTTT GGATCGGATC 
ACATCCCATT GGCTGCCGCT CGTCCGGGCC AAGTGCAATC CCTCCCTGGA CGGCGAGGGT 
GATGCCGAAG CGGAGGCGGA AGGAGACACA CAACGAGAGC CCATTCGGAA GCCAATCGTT 
CTGGTGGGTA ACAAAATAGA CCTCATCGAG TATTCCACCA TGGACAGCGT GCTGGCCATC 
ATGGAAGACT ACCCCGAGAT TGAGAGTTGC GTGGAGTGCT CTGCCAAGTC GCTGCACAAC 
ATCTCCGAGA TGTTTTACTA CGCCCAGAAG GCTGTGCTGC ACCCGACTTC TCCCCTGTAT 
ATGATGGAAG AACAGGAGCT CACATCCGCC TGCAAGAAGT CGCTGGTGCG CATTTTCAAG 
ATCTGCGACA TTGACGGGGA CAATCTGTTG AACGATTACG AGCTGAATTT ATTCCAGCGA 
CGCTGTTTCA ACACACCACT CCAGCCGCAA ATCCTTGATG AGGTGAAGGC CGTGATACAG 
AAAAATGTGC CCGATGGCAT ATACAACGAT GCGGTCACCC TGAAGGGCTT CCTCTTCTTA 
CACTGCCTTT TCATTCAGCG GGGAAGGAAC GAGACAACCT GGGCGGTGTT ACGGCGCTTT 
GGCTATAACG ACCAGTTGGA GATGTGCCAG GAGTATCTTA GGCCACCGCT GAAAATACCG 
CCTGGCAGCA GCACAGAACT CTCGCACCGC GGACAGCAGT TTCTGATTGC CGTGTTTGAG 
CGCTATGATC GCGATGGCGA CGGAGCCTTA TCACCTGAGG AGCACAAGAT GCTCTTCAGC 
ACATGCCCGG CTGCTCCGTG GTCCTACTCC ACCGACATAC GCAAGTCCTG CCCGATCAAC 
GAGACAACTG GATGGGTGAC ACTGCACGGG TGGCTATGTC GGTGGACACT GATGACGCTG 
ATTGATGTGG TCAAAACAAT GGAGTATTTG GCCTATTTGG GCTTCAATGT TCATGAAAAC 
GACAGCCAGT TGGCGGCAAT TCACGTAACT CGAGAGCGCC GCATCGATTT GGCCAAGCGC 
CAAAGCAGTC GGTCCGTTTA CAAGTGTCAT GTGATTGGAC CAAAGGGATC AGGAAAGACT 
GGAATGTGCA GGGGATTCCT GGTGGAGGAT ATGCACAAAC TAATCGGAAA AGAGTTTAAA 
ACGAATGTGG TTAATTGCAT CAACTCTGTG CAGGTGTATG GCCAGGAAAA GCACCTCATT 
CTGCGTGACA TAGACGTCAG GCATGCCCTC GATCCCCTCC AGCCACAAGA AGTCAATTGC 
GATGTTGCCT GCCTAGTCTA CGACTCATCT AATCCCCGTT CCTTTGAGTA CGTGGCCCGC 
ATCTACATCA AGTATTATGC GGAGAGCAAG ATTCCAGTGA TGATAGTCGG CACCAAGTGC 
GACATGGACG AACGCCGGCA GGACTATCTA ATGCAGCCCT CTGAATTCTG TGACAAGTAC 
AAGTTGCTGC CTCCGCATCT CTTCAGCCTG AAAACCAACA AAAAAGAACT GTATACCAAG 
CTGGCCACAA TGGCAGCGTT TCCTCGCTTC CAGGCCGCCT GGATACTGTT CTACAAGCAC 
AGGTTGGTAC AGCTGTGGGA GTCGGCTCAC CTGAGGCAAT TCGGCCTGAT GACGGAGGAC 
CCCAAGCTGT GGTTAAAGGC GGGACTAGGA GTGGCCGCAG CTACCATGCT GGGATTTATA 
GTGCTGAAAA CGATAAGTGC TGCCGGAGCC CACACCCGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262895.1
CDS	1238..3259
Translation	MGQYTASQRKNVRILLVGDAGVGKTSLILSLVSEEYPEEVPPRAEEITIPANVTPEQVPTSIVDFSAVEQSEDALAAEINKAHVVCIVYAVDDDDTLDRITSHWLPLVRAKCNPSLDGEGDAEAEAEGDTQREPIRKPIVLVGNKIDLIEYSTMDSVLAIMEDYPEIESCVECSAKSLHNISEMFYYAQKAVLHPTSPLYMMEEQELTSACKKSLVRIFKICDIDGDNLLNDYELNLFQRRCFNTPLQPQILDEVKAVIQKNVPDGIYNDAVTLKGFLFLHCLFIQRGRNETTWAVLRRFGYNDQLEMCQEYLRPPLKIPPGSSTELSHRGQQFLIAVFERYDRDGDGALSPEEHKMLFSTCPAAPWSYSTDIRKSCPINETTGWVTLHGWLCRWTLMTLIDVVKTMEYLAYLGFNVHENDSQLAAIHVTRERRIDLAKRQSSRSVYKCHVIGPKGSGKTGMCRGFLVEDMHKLIGKEFKTNVVNCINSVQVYGQEKHLILRDIDVRHALDPLQPQEVNCDVACLVYDSSNPRSFEYVARIYIKYYAESKIPVMIVGTKCDMDERRQDYLMQPSEFCDKYKLLPPHLFSLKTNKKELYTKLATMAAFPRFQAAWILFYKHRLVQLWESAHLRQFGLMTEDPKLWLKAGLGVAAATMLGFIVLKTISAAGAHTR

Target ORF information:

RefSeq Version	NM_001275966.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial Rho, transcript variant F (Miro), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275966.1

ORF Insert Sequence:

ATGGGACAGT ACACGGCGTC GCAGCGCAAG AATGTTAGGA TCCTGCTCGT CGGCGACGCC 
GGGGTGGGTA AGACGTCGTT GATTCTGTCT CTGGTCAGTG AGGAGTACCC GGAGGAGGTT 
CCGCCTAGGG CTGAGGAGAT TACCATTCCG GCGAACGTGA CGCCCGAGCA GGTGCCCACC 
AGCATAGTTG ACTTCTCGGC CGTGGAGCAG TCGGAGGATG CCCTGGCCGC CGAAATTAAC 
AAGGCGCACG TGGTGTGCAT AGTGTACGCC GTGGACGATG ACGACACTTT GGATCGGATC 
ACATCCCATT GGCTGCCGCT CGTCCGGGCC AAGTGCAATC CCTCCCTGGA CGGCGAGGGT 
GATGCCGAAG CGGAGGCGGA AGGAGACACA CAACGAGAGC CCATTCGGAA GCCAATCGTT 
CTGGTGGGTA ACAAAATAGA CCTCATCGAG TATTCCACCA TGGACAGCGT GCTGGCCATC 
ATGGAAGACT ACCCCGAGAT TGAGAGTTGC GTGGAGTGCT CTGCCAAGTC GCTGCACAAC 
ATCTCCGAGA TGTTTTACTA CGCCCAGAAG GCTGTGCTGC ACCCGACTTC TCCCCTGTAT 
ATGATGGAAG AACAGGAGCT CACATCCGCC TGCAAGAAGT CGCTGGTGCG CATTTTCAAG 
ATCTGCGACA TTGACGGGGA CAATCTGTTG AACGATTACG AGCTGAATTT ATTCCAGCGA 
CGCTGTTTCA ACACACCACT CCAGCCGCAA ATCCTTGATG AGGTGAAGGC CGTGATACAG 
AAAAATGTGC CCGATGGCAT ATACAACGAT GCGGTCACCC TGAAGGGCTT CCTCTTCTTA 
CACTGCCTTT TCATTCAGCG GGGAAGGAAC GAGACAACCT GGGCGGTGTT ACGGCGCTTT 
GGCTATAACG ACCAGTTGGA GATGTGCCAG GAGTATCTTA GGCCACCGCT GAAAATACCG 
CCTGGCAGCA GCACAGAACT CTCGCACCGC GGACAGCAGT TTCTGATTGC CGTGTTTGAG 
CGCTATGATC GCGATGGCGA CGGAGCCTTA TCACCTGAGG AGCACAAGAT GCTCTTCAGC 
ACATGCCCGG CTGCTCCGTG GTCCTACTCC ACCGACATAC GCAAGTCCTG CCCGATCAAC 
GAGACAACTG GATGGGTGAC ACTGCACGGG TGGCTATGTC GGTGGACACT GATGACGCTG 
ATTGATGTGG TCAAAACAAT GGAGTATTTG GCCTATTTGG GCTTCAATGT TCATGAAAAC 
GACAGCCAGT TGGCGGCAAT TCACGTAACT CGAGAGCGCC GCATCGATTT GGCCAAGCGC 
CAAAGCAGTC GGTCCGTTTA CAAGTGTCAT GTGATTGGAC CAAAGGGATC AGGAAAGACT 
GGAATGTGCA GGGGATTCCT GGTGGAGGAT ATGCACAAAC TAATCGGAAA AGAGTTTAAA 
ACGAATGTGG TTAATTGCAT CAACTCTGTG CAGGTGTATG GCCAGGAAAA GCACCTCATT 
CTGCGTGACA TAGACGTCAG GCATGCCCTC GATCCCCTCC AGCCACAAGA AGTCAATTGC 
GATGTTGCCT GCCTAGTCTA CGACTCATCT AATCCCCGTT CCTTTGAGTA CGTGGCCCGC 
ATCTACATCA AGTATTATGC GGAGAGCAAG ATTCCAGTGA TGATAGTCGG CACCAAGTGC 
GACATGGACG AACGCCGGCA GGACTATCTA ATGCAGCCCT CTGAATTCTG TGACAAGTAC 
AAGTTGCTGC CTCCGCATCT CTTCAGCCTG AAAACCAACA AAAAAGAACT GTATACCAAG 
CTGGCCACAA TGGCAGCGTT TCCTCGCTTC CAGGCCGCCT GGATACTGTT CTACAAGCAC 
AGGTTGGTAC AGCTGTGGGA GTCGGCTCAC CTGAGGCAAT TCGGCCTGAT GACGGAGGAC 
CCCAAGCTGT GGTTAAAGGC GGGACTAGGA GTGGCCGCAG CTACCATGCT GGGATTTATA 
GTGCTGAAAA CGATAAGTGC TGCCGGAGCC CACACCCGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Miro cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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