Gyc89Da cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gyc89Da
Entrez Gene ID	42001
Full Name	Guanylyl cyclase at 89Da
Synonyms	CG14885,Dmel\CG14885,GYCb,Gyc-89Da
General protein information	Preferred Names Guanylyl cyclase at 89Da Names CG14885 gene product from transcript CG14885-RB CG14885-PB CG14885-PC Gyc89Da-PB Gyc89Da-PC guanylyl cyclase at 89Da
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B17-89B17

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043253.1	NP_001036718.1	guanylyl cyclase at 89Da, isoform C
NM_001043254.2	NP_001036719.1	guanylyl cyclase at 89Da, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004564	XP_313866.5
Drosophila melanogaster (fruit fly)	Gyc-89Db	NP_650551.1
Drosophila melanogaster (fruit fly)	Gyc-89Da	NP_001036718.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gyc-89Da only forms an active guanylyl cyclase when co-expressed with Gyc-88E.
Title: Comparison of the properties of the five soluble guanylyl cyclase subunits in Drosophila melanogaster.

The following Gyc89Da gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gyc89Da cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05130	NM_001043253.1 Latest version!	Drosophila melanogaster guanylyl cyclase at 89Da, transcript variant C (Gyc89Da), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa05130	NM_001043254.2 Latest version!	Drosophila melanogaster guanylyl cyclase at 89Da, transcript variant B (Gyc89Da), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gyc89Da ( NM_001043253.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036718.1 Drosophila melanogaster guanylyl cyclase at 89Da, transcript variant C (Gyc89Da), mRNA.

Price & Availability↑

CloneID	OFa05130
Clone ID Related Accession (Same CDS sequence)	NM_001043253.1 , NM_001043254.2
Accession Version	NM_001043253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 89Da, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATGGAA TGCTGTATGA GAGTGTGCAG CACTACGTGC AGGAGGAGTA TGGCGTGGAC 
ATTTGGCGGA AGGTGTGTCA CATCATCGAC TGCAAGCACA ATAGCTTCAA GACGCACCAG 
ATCTATCCGG ACAAGCTGAT GCCGGATATC GCCGAGGCTT TGTCCGCCTG CACGGGCGAG 
TCCTTCGACT TCTGCATGAA CTTCTTCGGG CGCTGTTTTG TGCGATTCTT CAGCAACTTC 
GGCTACGACA AGATGATCCG CTCCACCGGG CGATACTTCT GCGACTTCCT GCAGTCCATC 
GACAACATTC ACTTGATTAT GCGTTTCACC TATCCCAAGA TGAAGTCGCC CAGCATGCAG 
TTGACCAATA TGGATGATAA CGGAGCTGTG ATCCTATACA GAAGCAGTCG CACTGGAATG 
TCCAAGTACC TAATTGGGCA GATGACCGAG GTGGCCCGCG AGTTTTACGG CCTGGAGATC 
AAGGCTTACG TGATCGAGAG CCAGAATGAC ATCAGTGGAG GCACTGCTGG TCCCATCAAG 
CTCACGGATG GTCCCTTGAC CGTGATTGTG AAGTATCGTC TGGACTTTGA TAACCGGGAG 
TACATGGCCA AGCGCGTCAA TACGGAGGCC CATCCCTCAC AGCTCAAAAT GCCCACCGTG 
AAACTGGATG TGTTCCTCGA TCTTTTTCCC TTCACATTTG TGCTGAACCA CGACATGAAG 
ATCACGCATG CTGGTGAGAA GATCGTTGAG ACCTGGATTA TGCACAATCC AGGGGCCAAT 
CCAAAGAGCT TCATTGGCAC CCACGTAATG GACTTGTTCC AATGCCGTCG TCCGAAAGAC 
ACCACTATCG ACTGGGATAC CCTGATCCAG ATGAGAGCCG TGCTCTTTGA GTTCGAGTTG 
ATTCGCACGG GTCACAATCG CGCTGCCTAC GATGCTGTCT TAAACATGGA CTTTGAGAAC 
TATGACGAGA TGGACTTAAA CGAAGCCCAA ACTATGGCGC TGGCCAAGGC TCAGGAGTTC 
AGTGAATCCC ATCCGGTGGA TGATGATGAA TCTGCGAGAG AGGATGAGAT CGACCCTGCC 
ACTGGGGAAC GTCGATCTTC CCAGGGCTTG AGGTCCATCC TTTTGAAGGG ACAGATGTTC 
TACATCAAAG ATGTGGACTC GCTGATCTTC CTGTGCAGTC CTCTAATCGA AAATCTCGAT 
GAGCTGCATG GAATCGGTCT CTATTTGAAC GATCTTAATC CACATGGACT GAGTAGAGAG 
CTTGTGATGG CGGGATGGCA GCACTGCTCC AAGCTGGAGA TCATGTTTGA AAAGGAGGAG 
CAGCGATCGG ATGAGCTGGA AAAATCGCTG GAGCTGGCAG ATTCCTGGAA GCGGCAGGGG 
GATGAGCTGC TCTACTCCAT GATTCCGCGG CCCATTGCCG AAAGAATGCG ACTCAGCGAA 
GAACAGGTTT GCCAGAGTTT CGAGGAAGTG TCCGTCATCT TCCTCGAGGT GATGAACGTC 
TATGATGAAG GATTGAACAG CATTCAGGGA GCTATGCAGA CGGTGAACAC TTTGAACAAA 
GTATTTTCCG CTCTGGATGA GGAGATCATT TCCCCATTCG TTTACAAAGT GGAGACCGTG 
GGCATGGTTT ACATGGCTGT TTCGGGTGCT CCTGATGTGA ATCCCCTGCA CGCCGAACAC 
GCCTGCGATT TGGCCTTAAG GGTGATGAAA AAGTTCAAGG CTCACGATAT GGGGGACGTG 
GCTATTCGAG TTGGCATCAA TTCGGGACCT GTGGTGGCTG GCGTTGTGGG CCAAAAGGTT 
CCTCGATACT GTCTGTTTGG GGACACCGTT AACACCGCCT CGCGAATGGA GAGCAGCAGT 
GATCCCTGGA AGATCCAGCT ATCGAAGTAC ACCGGCGATA AAGTGCGCCA AGTGGGCTAC 
AAAGTGGAGT CGCGTGGTAC GGTCCAGGTC AAAGGCAAGG GCGACATGGA GACCTACTGG 
CTGCTGGAGG GTCCCGAGGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036718.1
CDS	232..2235
Translation	MYGMLYESVQHYVQEEYGVDIWRKVCHIIDCKHNSFKTHQIYPDKLMPDIAEALSACTGESFDFCMNFFGRCFVRFFSNFGYDKMIRSTGRYFCDFLQSIDNIHLIMRFTYPKMKSPSMQLTNMDDNGAVILYRSSRTGMSKYLIGQMTEVAREFYGLEIKAYVIESQNDISGGTAGPIKLTDGPLTVIVKYRLDFDNREYMAKRVNTEAHPSQLKMPTVKLDVFLDLFPFTFVLNHDMKITHAGEKIVETWIMHNPGANPKSFIGTHVMDLFQCRRPKDTTIDWDTLIQMRAVLFEFELIRTGHNRAAYDAVLNMDFENYDEMDLNEAQTMALAKAQEFSESHPVDDDESAREDEIDPATGERRSSQGLRSILLKGQMFYIKDVDSLIFLCSPLIENLDELHGIGLYLNDLNPHGLSRELVMAGWQHCSKLEIMFEKEEQRSDELEKSLELADSWKRQGDELLYSMIPRPIAERMRLSEEQVCQSFEEVSVIFLEVMNVYDEGLNSIQGAMQTVNTLNKVFSALDEEIISPFVYKVETVGMVYMAVSGAPDVNPLHAEHACDLALRVMKKFKAHDMGDVAIRVGINSGPVVAGVVGQKVPRYCLFGDTVNTASRMESSSDPWKIQLSKYTGDKVRQVGYKVESRGTVQVKGKGDMETYWLLEGPEG

Target ORF information:

RefSeq Version	NM_001043253.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 89Da, transcript variant C (Gyc89Da), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043253.1

ORF Insert Sequence:

ATGTATGGAA TGCTGTATGA GAGTGTGCAG CACTACGTGC AGGAGGAGTA TGGCGTGGAC 
ATTTGGCGGA AGGTGTGTCA CATCATCGAC TGCAAGCACA ATAGCTTCAA GACGCACCAG 
ATCTATCCGG ACAAGCTGAT GCCGGATATC GCCGAGGCTT TGTCCGCCTG CACGGGCGAG 
TCCTTCGACT TCTGCATGAA CTTCTTCGGG CGCTGTTTTG TGCGATTCTT CAGCAACTTC 
GGCTACGACA AGATGATCCG CTCCACCGGG CGATACTTCT GCGACTTCCT GCAGTCCATC 
GACAACATTC ACTTGATTAT GCGTTTCACC TATCCCAAGA TGAAGTCGCC CAGCATGCAG 
TTGACCAATA TGGATGATAA CGGAGCTGTG ATCCTATACA GAAGCAGTCG CACTGGAATG 
TCCAAGTACC TAATTGGGCA GATGACCGAG GTGGCCCGCG AGTTTTACGG CCTGGAGATC 
AAGGCTTACG TGATCGAGAG CCAGAATGAC ATCAGTGGAG GCACTGCTGG TCCCATCAAG 
CTCACGGATG GTCCCTTGAC CGTGATTGTG AAGTATCGTC TGGACTTTGA TAACCGGGAG 
TACATGGCCA AGCGCGTCAA TACGGAGGCC CATCCCTCAC AGCTCAAAAT GCCCACCGTG 
AAACTGGATG TGTTCCTCGA TCTTTTTCCC TTCACATTTG TGCTGAACCA CGACATGAAG 
ATCACGCATG CTGGTGAGAA GATCGTTGAG ACCTGGATTA TGCACAATCC AGGGGCCAAT 
CCAAAGAGCT TCATTGGCAC CCACGTAATG GACTTGTTCC AATGCCGTCG TCCGAAAGAC 
ACCACTATCG ACTGGGATAC CCTGATCCAG ATGAGAGCCG TGCTCTTTGA GTTCGAGTTG 
ATTCGCACGG GTCACAATCG CGCTGCCTAC GATGCTGTCT TAAACATGGA CTTTGAGAAC 
TATGACGAGA TGGACTTAAA CGAAGCCCAA ACTATGGCGC TGGCCAAGGC TCAGGAGTTC 
AGTGAATCCC ATCCGGTGGA TGATGATGAA TCTGCGAGAG AGGATGAGAT CGACCCTGCC 
ACTGGGGAAC GTCGATCTTC CCAGGGCTTG AGGTCCATCC TTTTGAAGGG ACAGATGTTC 
TACATCAAAG ATGTGGACTC GCTGATCTTC CTGTGCAGTC CTCTAATCGA AAATCTCGAT 
GAGCTGCATG GAATCGGTCT CTATTTGAAC GATCTTAATC CACATGGACT GAGTAGAGAG 
CTTGTGATGG CGGGATGGCA GCACTGCTCC AAGCTGGAGA TCATGTTTGA AAAGGAGGAG 
CAGCGATCGG ATGAGCTGGA AAAATCGCTG GAGCTGGCAG ATTCCTGGAA GCGGCAGGGG 
GATGAGCTGC TCTACTCCAT GATTCCGCGG CCCATTGCCG AAAGAATGCG ACTCAGCGAA 
GAACAGGTTT GCCAGAGTTT CGAGGAAGTG TCCGTCATCT TCCTCGAGGT GATGAACGTC 
TATGATGAAG GATTGAACAG CATTCAGGGA GCTATGCAGA CGGTGAACAC TTTGAACAAA 
GTATTTTCCG CTCTGGATGA GGAGATCATT TCCCCATTCG TTTACAAAGT GGAGACCGTG 
GGCATGGTTT ACATGGCTGT TTCGGGTGCT CCTGATGTGA ATCCCCTGCA CGCCGAACAC 
GCCTGCGATT TGGCCTTAAG GGTGATGAAA AAGTTCAAGG CTCACGATAT GGGGGACGTG 
GCTATTCGAG TTGGCATCAA TTCGGGACCT GTGGTGGCTG GCGTTGTGGG CCAAAAGGTT 
CCTCGATACT GTCTGTTTGG GGACACCGTT AACACCGCCT CGCGAATGGA GAGCAGCAGT 
GATCCCTGGA AGATCCAGCT ATCGAAGTAC ACCGGCGATA AAGTGCGCCA AGTGGGCTAC 
AAAGTGGAGT CGCGTGGTAC GGTCCAGGTC AAAGGCAAGG GCGACATGGA GACCTACTGG 
CTGCTGGAGG GTCCCGAGGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyc89Da ( NM_001043254.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036719.1 Drosophila melanogaster guanylyl cyclase at 89Da, transcript variant B (Gyc89Da), mRNA.

Price & Availability↑

CloneID	OFa05130
Clone ID Related Accession (Same CDS sequence)	NM_001043253.1 , NM_001043254.2
Accession Version	NM_001043254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 89Da, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001043254.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATGGAA TGCTGTATGA GAGTGTGCAG CACTACGTGC AGGAGGAGTA TGGCGTGGAC 
ATTTGGCGGA AGGTGTGTCA CATCATCGAC TGCAAGCACA ATAGCTTCAA GACGCACCAG 
ATCTATCCGG ACAAGCTGAT GCCGGATATC GCCGAGGCTT TGTCCGCCTG CACGGGCGAG 
TCCTTCGACT TCTGCATGAA CTTCTTCGGG CGCTGTTTTG TGCGATTCTT CAGCAACTTC 
GGCTACGACA AGATGATCCG CTCCACCGGG CGATACTTCT GCGACTTCCT GCAGTCCATC 
GACAACATTC ACTTGATTAT GCGTTTCACC TATCCCAAGA TGAAGTCGCC CAGCATGCAG 
TTGACCAATA TGGATGATAA CGGAGCTGTG ATCCTATACA GAAGCAGTCG CACTGGAATG 
TCCAAGTACC TAATTGGGCA GATGACCGAG GTGGCCCGCG AGTTTTACGG CCTGGAGATC 
AAGGCTTACG TGATCGAGAG CCAGAATGAC ATCAGTGGAG GCACTGCTGG TCCCATCAAG 
CTCACGGATG GTCCCTTGAC CGTGATTGTG AAGTATCGTC TGGACTTTGA TAACCGGGAG 
TACATGGCCA AGCGCGTCAA TACGGAGGCC CATCCCTCAC AGCTCAAAAT GCCCACCGTG 
AAACTGGATG TGTTCCTCGA TCTTTTTCCC TTCACATTTG TGCTGAACCA CGACATGAAG 
ATCACGCATG CTGGTGAGAA GATCGTTGAG ACCTGGATTA TGCACAATCC AGGGGCCAAT 
CCAAAGAGCT TCATTGGCAC CCACGTAATG GACTTGTTCC AATGCCGTCG TCCGAAAGAC 
ACCACTATCG ACTGGGATAC CCTGATCCAG ATGAGAGCCG TGCTCTTTGA GTTCGAGTTG 
ATTCGCACGG GTCACAATCG CGCTGCCTAC GATGCTGTCT TAAACATGGA CTTTGAGAAC 
TATGACGAGA TGGACTTAAA CGAAGCCCAA ACTATGGCGC TGGCCAAGGC TCAGGAGTTC 
AGTGAATCCC ATCCGGTGGA TGATGATGAA TCTGCGAGAG AGGATGAGAT CGACCCTGCC 
ACTGGGGAAC GTCGATCTTC CCAGGGCTTG AGGTCCATCC TTTTGAAGGG ACAGATGTTC 
TACATCAAAG ATGTGGACTC GCTGATCTTC CTGTGCAGTC CTCTAATCGA AAATCTCGAT 
GAGCTGCATG GAATCGGTCT CTATTTGAAC GATCTTAATC CACATGGACT GAGTAGAGAG 
CTTGTGATGG CGGGATGGCA GCACTGCTCC AAGCTGGAGA TCATGTTTGA AAAGGAGGAG 
CAGCGATCGG ATGAGCTGGA AAAATCGCTG GAGCTGGCAG ATTCCTGGAA GCGGCAGGGG 
GATGAGCTGC TCTACTCCAT GATTCCGCGG CCCATTGCCG AAAGAATGCG ACTCAGCGAA 
GAACAGGTTT GCCAGAGTTT CGAGGAAGTG TCCGTCATCT TCCTCGAGGT GATGAACGTC 
TATGATGAAG GATTGAACAG CATTCAGGGA GCTATGCAGA CGGTGAACAC TTTGAACAAA 
GTATTTTCCG CTCTGGATGA GGAGATCATT TCCCCATTCG TTTACAAAGT GGAGACCGTG 
GGCATGGTTT ACATGGCTGT TTCGGGTGCT CCTGATGTGA ATCCCCTGCA CGCCGAACAC 
GCCTGCGATT TGGCCTTAAG GGTGATGAAA AAGTTCAAGG CTCACGATAT GGGGGACGTG 
GCTATTCGAG TTGGCATCAA TTCGGGACCT GTGGTGGCTG GCGTTGTGGG CCAAAAGGTT 
CCTCGATACT GTCTGTTTGG GGACACCGTT AACACCGCCT CGCGAATGGA GAGCAGCAGT 
GATCCCTGGA AGATCCAGCT ATCGAAGTAC ACCGGCGATA AAGTGCGCCA AGTGGGCTAC 
AAAGTGGAGT CGCGTGGTAC GGTCCAGGTC AAAGGCAAGG GCGACATGGA GACCTACTGG 
CTGCTGGAGG GTCCCGAGGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036719.1
CDS	167..2170
Translation	MYGMLYESVQHYVQEEYGVDIWRKVCHIIDCKHNSFKTHQIYPDKLMPDIAEALSACTGESFDFCMNFFGRCFVRFFSNFGYDKMIRSTGRYFCDFLQSIDNIHLIMRFTYPKMKSPSMQLTNMDDNGAVILYRSSRTGMSKYLIGQMTEVAREFYGLEIKAYVIESQNDISGGTAGPIKLTDGPLTVIVKYRLDFDNREYMAKRVNTEAHPSQLKMPTVKLDVFLDLFPFTFVLNHDMKITHAGEKIVETWIMHNPGANPKSFIGTHVMDLFQCRRPKDTTIDWDTLIQMRAVLFEFELIRTGHNRAAYDAVLNMDFENYDEMDLNEAQTMALAKAQEFSESHPVDDDESAREDEIDPATGERRSSQGLRSILLKGQMFYIKDVDSLIFLCSPLIENLDELHGIGLYLNDLNPHGLSRELVMAGWQHCSKLEIMFEKEEQRSDELEKSLELADSWKRQGDELLYSMIPRPIAERMRLSEEQVCQSFEEVSVIFLEVMNVYDEGLNSIQGAMQTVNTLNKVFSALDEEIISPFVYKVETVGMVYMAVSGAPDVNPLHAEHACDLALRVMKKFKAHDMGDVAIRVGINSGPVVAGVVGQKVPRYCLFGDTVNTASRMESSSDPWKIQLSKYTGDKVRQVGYKVESRGTVQVKGKGDMETYWLLEGPEG

Target ORF information:

RefSeq Version	NM_001043254.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 89Da, transcript variant B (Gyc89Da), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043254.2

ORF Insert Sequence:

ATGTATGGAA TGCTGTATGA GAGTGTGCAG CACTACGTGC AGGAGGAGTA TGGCGTGGAC 
ATTTGGCGGA AGGTGTGTCA CATCATCGAC TGCAAGCACA ATAGCTTCAA GACGCACCAG 
ATCTATCCGG ACAAGCTGAT GCCGGATATC GCCGAGGCTT TGTCCGCCTG CACGGGCGAG 
TCCTTCGACT TCTGCATGAA CTTCTTCGGG CGCTGTTTTG TGCGATTCTT CAGCAACTTC 
GGCTACGACA AGATGATCCG CTCCACCGGG CGATACTTCT GCGACTTCCT GCAGTCCATC 
GACAACATTC ACTTGATTAT GCGTTTCACC TATCCCAAGA TGAAGTCGCC CAGCATGCAG 
TTGACCAATA TGGATGATAA CGGAGCTGTG ATCCTATACA GAAGCAGTCG CACTGGAATG 
TCCAAGTACC TAATTGGGCA GATGACCGAG GTGGCCCGCG AGTTTTACGG CCTGGAGATC 
AAGGCTTACG TGATCGAGAG CCAGAATGAC ATCAGTGGAG GCACTGCTGG TCCCATCAAG 
CTCACGGATG GTCCCTTGAC CGTGATTGTG AAGTATCGTC TGGACTTTGA TAACCGGGAG 
TACATGGCCA AGCGCGTCAA TACGGAGGCC CATCCCTCAC AGCTCAAAAT GCCCACCGTG 
AAACTGGATG TGTTCCTCGA TCTTTTTCCC TTCACATTTG TGCTGAACCA CGACATGAAG 
ATCACGCATG CTGGTGAGAA GATCGTTGAG ACCTGGATTA TGCACAATCC AGGGGCCAAT 
CCAAAGAGCT TCATTGGCAC CCACGTAATG GACTTGTTCC AATGCCGTCG TCCGAAAGAC 
ACCACTATCG ACTGGGATAC CCTGATCCAG ATGAGAGCCG TGCTCTTTGA GTTCGAGTTG 
ATTCGCACGG GTCACAATCG CGCTGCCTAC GATGCTGTCT TAAACATGGA CTTTGAGAAC 
TATGACGAGA TGGACTTAAA CGAAGCCCAA ACTATGGCGC TGGCCAAGGC TCAGGAGTTC 
AGTGAATCCC ATCCGGTGGA TGATGATGAA TCTGCGAGAG AGGATGAGAT CGACCCTGCC 
ACTGGGGAAC GTCGATCTTC CCAGGGCTTG AGGTCCATCC TTTTGAAGGG ACAGATGTTC 
TACATCAAAG ATGTGGACTC GCTGATCTTC CTGTGCAGTC CTCTAATCGA AAATCTCGAT 
GAGCTGCATG GAATCGGTCT CTATTTGAAC GATCTTAATC CACATGGACT GAGTAGAGAG 
CTTGTGATGG CGGGATGGCA GCACTGCTCC AAGCTGGAGA TCATGTTTGA AAAGGAGGAG 
CAGCGATCGG ATGAGCTGGA AAAATCGCTG GAGCTGGCAG ATTCCTGGAA GCGGCAGGGG 
GATGAGCTGC TCTACTCCAT GATTCCGCGG CCCATTGCCG AAAGAATGCG ACTCAGCGAA 
GAACAGGTTT GCCAGAGTTT CGAGGAAGTG TCCGTCATCT TCCTCGAGGT GATGAACGTC 
TATGATGAAG GATTGAACAG CATTCAGGGA GCTATGCAGA CGGTGAACAC TTTGAACAAA 
GTATTTTCCG CTCTGGATGA GGAGATCATT TCCCCATTCG TTTACAAAGT GGAGACCGTG 
GGCATGGTTT ACATGGCTGT TTCGGGTGCT CCTGATGTGA ATCCCCTGCA CGCCGAACAC 
GCCTGCGATT TGGCCTTAAG GGTGATGAAA AAGTTCAAGG CTCACGATAT GGGGGACGTG 
GCTATTCGAG TTGGCATCAA TTCGGGACCT GTGGTGGCTG GCGTTGTGGG CCAAAAGGTT 
CCTCGATACT GTCTGTTTGG GGACACCGTT AACACCGCCT CGCGAATGGA GAGCAGCAGT 
GATCCCTGGA AGATCCAGCT ATCGAAGTAC ACCGGCGATA AAGTGCGCCA AGTGGGCTAC 
AAAGTGGAGT CGCGTGGTAC GGTCCAGGTC AAAGGCAAGG GCGACATGGA GACCTACTGG 
CTGCTGGAGG GTCCCGAGGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gyc89Da cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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