Nsf2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nsf2
Entrez Gene ID	41694
Full Name	N-ethylmaleimide-sensitive factor 2
Synonyms	CG33101,CG9931,DmNSF2,Dmel\CG33101,EC3-11,NSF-2,NSF2,Nsf-2,dNSF,dNSF-2,dNSF2,dNsf2,dnsf2,lotus
General protein information	Preferred Names N-ethylmaleimide-sensitive factor 2 Names CG33101 gene product from transcript CG33101-RC CG33101-PA CG33101-PB CG33101-PC N-ethylmaleimide sensitive factor 2 NEM-sensitive fusion protein 2 Nsf2-PA Nsf2-PB Nsf2-PC lotus
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87F15-87F15

mRNA and Protein(s)

mRNA	Protein	Name
NM_176499.2	NP_788676.1	N-ethylmaleimide-sensitive factor 2, isoform A
NM_001300390.1	NP_001287319.1	N-ethylmaleimide-sensitive factor 2, isoform C
NM_001300389.1	NP_001287318.1	N-ethylmaleimide-sensitive factor 2, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NSF	XP_511626.3
Canis lupus familiaris (dog)	NSF	XP_548044.2
Mus musculus (house mouse)	Nsf	NP_032766.2
Drosophila melanogaster (fruit fly)	comt	NP_001259506.1
Saccharomyces cerevisiae (baker's yeast)	SEC18	NP_009636.3
Gallus gallus (chicken)	NSF	NP_001019627.1
Danio rerio (zebrafish)	nsfa	NP_001037793.1
Neurospora crassa	NCU03387	XP_956834.1
Xenopus tropicalis (tropical clawed frog)	nsf	NP_001072788.1
Bos taurus (cattle)	NSF	XP_002696103.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012684	XP_307148.3
Schizosaccharomyces pombe (fission yeast)	sec18	NP_594690.1
Magnaporthe oryzae (rice blast fungus)	MGG_02418	XP_003709204.1
	NSF	XP_002800596.1
Caenorhabditis elegans (roundworm)	nsf-1	NP_001076603.1
Caenorhabditis elegans (roundworm)	nsf-1	NP_001076604.1
Homo sapiens (human)	NSF	NP_006169.2
Rattus norvegicus (Norway rat)	Nsf	NP_068516.1
Drosophila melanogaster (fruit fly)	Nsf2	NP_788676.1
Kluyveromyces lactis	KLLA0C14520g	XP_452850.1
Eremothecium gossypii	AGOS_AER169C	NP_985027.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CCM3 and GCKIII prevent tube dilation in Drosophila trachea; tube dilation is suppressed by reduction of NSF2
Title: Focal defects in single-celled tubes mutant for Cerebral cavernous malformation 3, GCKIII, or NSF2.

Interaction of cytoskeleton genes with NSF2-induced neuromuscular junction overgrowth suggests that NSF may function near the top of the actin regulatory pathway.
Title: Interaction of cytoskeleton genes with NSF2-induced neuromuscular junction overgrowth.

These data are consistent with a model in which actin filaments promote vesicle mobility and suggest that N-ethylmaleimide sensitive factor participates in establishing or maintaining this population of actin.
Title: Synaptic vesicle mobility and presynaptic F-actin are disrupted in a N-ethylmaleimide-sensitive factor allele of Drosophila.

NSF2(E/Q) larval synapses indicate the NMJ overgrowth phenotype is present at the embryonic neuromuscular synapse. At the ultrastructural level, considerable alterations in the number and distribution of synapses and active zones were found.
Title: Disruption of synaptic development and ultrastructure by Drosophila NSF2 alleles.

genetic analysis of suppressors of NSF2 neuromuscular junction overgrowth
Title: A genetic screen for suppressors of Drosophila NSF2 neuromuscular junction overgrowth.

The following Nsf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nsf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08365	NM_001300389.1 Latest version!	Drosophila melanogaster N-ethylmaleimide-sensitive factor 2 (Nsf2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa08365	NM_176499.2 Latest version!	Drosophila melanogaster N-ethylmaleimide-sensitive factor 2 (Nsf2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa08365	NM_001300390.1 Latest version!	Drosophila melanogaster N-ethylmaleimide-sensitive factor 2 (Nsf2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nsf2 ( NM_176499.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788676.1 Drosophila melanogaster N-ethylmaleimide-sensitive factor 2 (Nsf2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa08365
Clone ID Related Accession (Same CDS sequence)	NM_176499.2 , NM_001300390.1 , NM_001300389.1
Accession Version	NM_176499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	N-ethylmaleimide-sensitive factor 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_176499.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAATTG AAAAAGCCCA CCGGATGAGA GCGATCAAAT GCCCAACGGA TGAGCTGTCC 
CTGACGAACA AGGCGATTGT GAATGTGTCC GATTTCACGG AGGAGGTCAA ATATGTGGAC 
ATCTCGCCGG GCCCGGGGCT GCACTACATC TTCGCGCTGG AGAAGATCAG CGGGCCGGAG 
CTGCCGCTCG GCCACGTCGG ATTCTCGCTG GTCCAGCGCA AGTGGGCCAC GCTCTCCATC 
AACCAGGAAA TAGACGTGCG GCCGTACCGA TTCGATGCCA GCGCCGATAT CATCACACTG 
GTCTCCTTCG AGACGGACTT CCTGCAGAAG AAGACCACCA CGCAGGAGCC CTACGACTCC 
GACGAGATGG CCAAGGAGTT CCTCATGCAG TTCGCCGGAA TGCCGCTGAC CGTCGGCCAA 
ACGCTCGTCT TTCAGTTCAA AGACAAGAAA TTCCTGGGCC TCGCTGTAAA AACGCTGGAG 
GCAGTGGATC CCCGAACAGT GGGCGATTCC CTACCGAAGA CTCGGAACGT ACGATTCGGC 
CGCATCCTCG GTAACACGGT GGTGCAGTTC GAGAAGGCCG AAAACTCTGT GCTAAATCTG 
CAGGGTCGCT CCAAGGGTAA GATCGTGCGG CAGTCCATCA TAAATCCGGA CTGGGACTTT 
GGCAAGATGG GCATTGGTGG CCTAGACAAA GAGTTCAATG CGATTTTCCG ACGCGCCTTC 
GCCTCTCGTG TTTTTCCGCC GGAACTTGTC GAACAACTGG GCATCAAGCA CGTTAAGGGT 
ATTCTGTTGT ACGGACCACC AGGCACGGGA AAAACCCTGA TGGCCCGGCA AATAGGTACC 
ATGCTGAATG CCCGCGAACC CAAGATCGTG AATGGACCGC AGATCCTGGA CAAGTACGTG 
GGTGAATCAG AAGCCAATAT CCGGCGACTG TTTGCCGAGG CCGAGGAGGA GGAAAAGCGC 
TTGGGACCGA ACAGTGGACT GCACATCATC ATCTTCGACG AGATCGACGC CATTTGCAAG 
GCTCGCGGAT CCGTGGCCGG TAATAGTGGT GTCCACGACA CGGTCGTCAA TCAACTGCTA 
GCCAAGATCG ACGGCGTGGA GCAGTTGAAC AACATCCTGG TAATAGGAAT GACCAACCGG 
CGGGACATGA TCGATGAGGC CCTGCTGCGA CCTGGACGCT TGGAAGTGCA AATGGAGATC 
AGCTTGCCCA ACGAGCAGGG TCGCGTCCAG ATCCTGAATA TCCACACCAA GCGGATGCGT 
GACTTTAACA AGATAGCTAG CGATGTGGAC AACAATGAGA TCGCGGCCAA GACAAAGAAC 
TTCAGTGGCG CAGAGTTGGA GGGTCTGGTT AGAGCGGCCC AATCCACGGC CATGAATCGG 
TTAATAAAGG CGGACTCCAA GGTTCACGTG GACCCGGAGG CTATGGAAAA ACTGCGTGTT 
ACGCGAGCCG ACTTTCTGCA CGCATTGGAC AACGACATCA AGCCCGCCTT TGGAGCAGCC 
CAGGAGATGC TGGAAAATCT GCTGGCACGC GGAATTATAA ACTGGGGCCC ACCGGTCACC 
GAACTCCTGG AGGACGGAAT GCTCTCCGTA CAGCAGGCGA AGGCCACTGA GAGCTCTGGC 
CTGGTGTCTG TTCTCATCGA AGGAGCTCCC AACTCCGGCA AATCAGCGCT GGCCGCTAAT 
CTGGCCCAGT TGAGTGATTT CCCATTCGTT AAGGTCTGCT CCCCGGAAGA TATGGTAGGA 
TTTACGGAGT CAGCCAAGTG CCTGCACATC CGCAAGATCT TCGACGACGC CTATCGCTCC 
ACATTGAGCT GCATTGTGGT GGACAATGTG GAGCGATTGC TAGACTACGG TCCAATTGGA 
CCCAGGTACT CTAATCTCAC GCTGCAGGCG CTATTGGTTC TACTGAAAAA GCAACCGCCA 
AAGGGTAGAA AACTGCTGAT CCTGTGCACT TCCAGCCGGC GGGACGTTCT CGAGGAGATG 
GAAATGCTCT CGGCTTTCAC GTCCGTCCTG CATGTGTCCA ATCTCTCAAC TCCCGAGAAC 
GTGCTTGCCG TGCTGGATGA TTCTGATTTG TTTAGTCCCG AGGAGCTGCA GAGCATTGCC 
AGGAAAATGG CGGGTAAGCG ATTGTGTATC GGCATCAAGA AGCTGCTGGC TCTCATCGAT 
ATGATCCGGC AATCGGAGCC TCACCAGCGG GTTATCAAGT TCCTCAGCAA AATGGAGGAG 
GAGGGCGGTC TGGAAATGGA TAGGGTGCAA GGGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788676.1
CDS	80..2338
Translation	MSIEKAHRMRAIKCPTDELSLTNKAIVNVSDFTEEVKYVDISPGPGLHYIFALEKISGPELPLGHVGFSLVQRKWATLSINQEIDVRPYRFDASADIITLVSFETDFLQKKTTTQEPYDSDEMAKEFLMQFAGMPLTVGQTLVFQFKDKKFLGLAVKTLEAVDPRTVGDSLPKTRNVRFGRILGNTVVQFEKAENSVLNLQGRSKGKIVRQSIINPDWDFGKMGIGGLDKEFNAIFRRAFASRVFPPELVEQLGIKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANIRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKARGSVAGNSGVHDTVVNQLLAKIDGVEQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMRDFNKIASDVDNNEIAAKTKNFSGAELEGLVRAAQSTAMNRLIKADSKVHVDPEAMEKLRVTRADFLHALDNDIKPAFGAAQEMLENLLARGIINWGPPVTELLEDGMLSVQQAKATESSGLVSVLIEGAPNSGKSALAANLAQLSDFPFVKVCSPEDMVGFTESAKCLHIRKIFDDAYRSTLSCIVVDNVERLLDYGPIGPRYSNLTLQALLVLLKKQPPKGRKLLILCTSSRRDVLEEMEMLSAFTSVLHVSNLSTPENVLAVLDDSDLFSPEELQSIARKMAGKRLCIGIKKLLALIDMIRQSEPHQRVIKFLSKMEEEGGLEMDRVQGH

Target ORF information:

RefSeq Version	NM_176499.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster N-ethylmaleimide-sensitive factor 2 (Nsf2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176499.2

ORF Insert Sequence:

ATGTCAATTG AAAAAGCCCA CCGGATGAGA GCGATCAAAT GCCCAACGGA TGAGCTGTCC 
CTGACGAACA AGGCGATTGT GAATGTGTCC GATTTCACGG AGGAGGTCAA ATATGTGGAC 
ATCTCGCCGG GCCCGGGGCT GCACTACATC TTCGCGCTGG AGAAGATCAG CGGGCCGGAG 
CTGCCGCTCG GCCACGTCGG ATTCTCGCTG GTCCAGCGCA AGTGGGCCAC GCTCTCCATC 
AACCAGGAAA TAGACGTGCG GCCGTACCGA TTCGATGCCA GCGCCGATAT CATCACACTG 
GTCTCCTTCG AGACGGACTT CCTGCAGAAG AAGACCACCA CGCAGGAGCC CTACGACTCC 
GACGAGATGG CCAAGGAGTT CCTCATGCAG TTCGCCGGAA TGCCGCTGAC CGTCGGCCAA 
ACGCTCGTCT TTCAGTTCAA AGACAAGAAA TTCCTGGGCC TCGCTGTAAA AACGCTGGAG 
GCAGTGGATC CCCGAACAGT GGGCGATTCC CTACCGAAGA CTCGGAACGT ACGATTCGGC 
CGCATCCTCG GTAACACGGT GGTGCAGTTC GAGAAGGCCG AAAACTCTGT GCTAAATCTG 
CAGGGTCGCT CCAAGGGTAA GATCGTGCGG CAGTCCATCA TAAATCCGGA CTGGGACTTT 
GGCAAGATGG GCATTGGTGG CCTAGACAAA GAGTTCAATG CGATTTTCCG ACGCGCCTTC 
GCCTCTCGTG TTTTTCCGCC GGAACTTGTC GAACAACTGG GCATCAAGCA CGTTAAGGGT 
ATTCTGTTGT ACGGACCACC AGGCACGGGA AAAACCCTGA TGGCCCGGCA AATAGGTACC 
ATGCTGAATG CCCGCGAACC CAAGATCGTG AATGGACCGC AGATCCTGGA CAAGTACGTG 
GGTGAATCAG AAGCCAATAT CCGGCGACTG TTTGCCGAGG CCGAGGAGGA GGAAAAGCGC 
TTGGGACCGA ACAGTGGACT GCACATCATC ATCTTCGACG AGATCGACGC CATTTGCAAG 
GCTCGCGGAT CCGTGGCCGG TAATAGTGGT GTCCACGACA CGGTCGTCAA TCAACTGCTA 
GCCAAGATCG ACGGCGTGGA GCAGTTGAAC AACATCCTGG TAATAGGAAT GACCAACCGG 
CGGGACATGA TCGATGAGGC CCTGCTGCGA CCTGGACGCT TGGAAGTGCA AATGGAGATC 
AGCTTGCCCA ACGAGCAGGG TCGCGTCCAG ATCCTGAATA TCCACACCAA GCGGATGCGT 
GACTTTAACA AGATAGCTAG CGATGTGGAC AACAATGAGA TCGCGGCCAA GACAAAGAAC 
TTCAGTGGCG CAGAGTTGGA GGGTCTGGTT AGAGCGGCCC AATCCACGGC CATGAATCGG 
TTAATAAAGG CGGACTCCAA GGTTCACGTG GACCCGGAGG CTATGGAAAA ACTGCGTGTT 
ACGCGAGCCG ACTTTCTGCA CGCATTGGAC AACGACATCA AGCCCGCCTT TGGAGCAGCC 
CAGGAGATGC TGGAAAATCT GCTGGCACGC GGAATTATAA ACTGGGGCCC ACCGGTCACC 
GAACTCCTGG AGGACGGAAT GCTCTCCGTA CAGCAGGCGA AGGCCACTGA GAGCTCTGGC 
CTGGTGTCTG TTCTCATCGA AGGAGCTCCC AACTCCGGCA AATCAGCGCT GGCCGCTAAT 
CTGGCCCAGT TGAGTGATTT CCCATTCGTT AAGGTCTGCT CCCCGGAAGA TATGGTAGGA 
TTTACGGAGT CAGCCAAGTG CCTGCACATC CGCAAGATCT TCGACGACGC CTATCGCTCC 
ACATTGAGCT GCATTGTGGT GGACAATGTG GAGCGATTGC TAGACTACGG TCCAATTGGA 
CCCAGGTACT CTAATCTCAC GCTGCAGGCG CTATTGGTTC TACTGAAAAA GCAACCGCCA 
AAGGGTAGAA AACTGCTGAT CCTGTGCACT TCCAGCCGGC GGGACGTTCT CGAGGAGATG 
GAAATGCTCT CGGCTTTCAC GTCCGTCCTG CATGTGTCCA ATCTCTCAAC TCCCGAGAAC 
GTGCTTGCCG TGCTGGATGA TTCTGATTTG TTTAGTCCCG AGGAGCTGCA GAGCATTGCC 
AGGAAAATGG CGGGTAAGCG ATTGTGTATC GGCATCAAGA AGCTGCTGGC TCTCATCGAT 
ATGATCCGGC AATCGGAGCC TCACCAGCGG GTTATCAAGT TCCTCAGCAA AATGGAGGAG 
GAGGGCGGTC TGGAAATGGA TAGGGTGCAA GGGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsf2 ( NM_001300390.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287319.1 Drosophila melanogaster N-ethylmaleimide-sensitive factor 2 (Nsf2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08365
Clone ID Related Accession (Same CDS sequence)	NM_176499.2 , NM_001300390.1 , NM_001300389.1
Accession Version	NM_001300390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	N-ethylmaleimide-sensitive factor 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAATTG AAAAAGCCCA CCGGATGAGA GCGATCAAAT GCCCAACGGA TGAGCTGTCC 
CTGACGAACA AGGCGATTGT GAATGTGTCC GATTTCACGG AGGAGGTCAA ATATGTGGAC 
ATCTCGCCGG GCCCGGGGCT GCACTACATC TTCGCGCTGG AGAAGATCAG CGGGCCGGAG 
CTGCCGCTCG GCCACGTCGG ATTCTCGCTG GTCCAGCGCA AGTGGGCCAC GCTCTCCATC 
AACCAGGAAA TAGACGTGCG GCCGTACCGA TTCGATGCCA GCGCCGATAT CATCACACTG 
GTCTCCTTCG AGACGGACTT CCTGCAGAAG AAGACCACCA CGCAGGAGCC CTACGACTCC 
GACGAGATGG CCAAGGAGTT CCTCATGCAG TTCGCCGGAA TGCCGCTGAC CGTCGGCCAA 
ACGCTCGTCT TTCAGTTCAA AGACAAGAAA TTCCTGGGCC TCGCTGTAAA AACGCTGGAG 
GCAGTGGATC CCCGAACAGT GGGCGATTCC CTACCGAAGA CTCGGAACGT ACGATTCGGC 
CGCATCCTCG GTAACACGGT GGTGCAGTTC GAGAAGGCCG AAAACTCTGT GCTAAATCTG 
CAGGGTCGCT CCAAGGGTAA GATCGTGCGG CAGTCCATCA TAAATCCGGA CTGGGACTTT 
GGCAAGATGG GCATTGGTGG CCTAGACAAA GAGTTCAATG CGATTTTCCG ACGCGCCTTC 
GCCTCTCGTG TTTTTCCGCC GGAACTTGTC GAACAACTGG GCATCAAGCA CGTTAAGGGT 
ATTCTGTTGT ACGGACCACC AGGCACGGGA AAAACCCTGA TGGCCCGGCA AATAGGTACC 
ATGCTGAATG CCCGCGAACC CAAGATCGTG AATGGACCGC AGATCCTGGA CAAGTACGTG 
GGTGAATCAG AAGCCAATAT CCGGCGACTG TTTGCCGAGG CCGAGGAGGA GGAAAAGCGC 
TTGGGACCGA ACAGTGGACT GCACATCATC ATCTTCGACG AGATCGACGC CATTTGCAAG 
GCTCGCGGAT CCGTGGCCGG TAATAGTGGT GTCCACGACA CGGTCGTCAA TCAACTGCTA 
GCCAAGATCG ACGGCGTGGA GCAGTTGAAC AACATCCTGG TAATAGGAAT GACCAACCGG 
CGGGACATGA TCGATGAGGC CCTGCTGCGA CCTGGACGCT TGGAAGTGCA AATGGAGATC 
AGCTTGCCCA ACGAGCAGGG TCGCGTCCAG ATCCTGAATA TCCACACCAA GCGGATGCGT 
GACTTTAACA AGATAGCTAG CGATGTGGAC AACAATGAGA TCGCGGCCAA GACAAAGAAC 
TTCAGTGGCG CAGAGTTGGA GGGTCTGGTT AGAGCGGCCC AATCCACGGC CATGAATCGG 
TTAATAAAGG CGGACTCCAA GGTTCACGTG GACCCGGAGG CTATGGAAAA ACTGCGTGTT 
ACGCGAGCCG ACTTTCTGCA CGCATTGGAC AACGACATCA AGCCCGCCTT TGGAGCAGCC 
CAGGAGATGC TGGAAAATCT GCTGGCACGC GGAATTATAA ACTGGGGCCC ACCGGTCACC 
GAACTCCTGG AGGACGGAAT GCTCTCCGTA CAGCAGGCGA AGGCCACTGA GAGCTCTGGC 
CTGGTGTCTG TTCTCATCGA AGGAGCTCCC AACTCCGGCA AATCAGCGCT GGCCGCTAAT 
CTGGCCCAGT TGAGTGATTT CCCATTCGTT AAGGTCTGCT CCCCGGAAGA TATGGTAGGA 
TTTACGGAGT CAGCCAAGTG CCTGCACATC CGCAAGATCT TCGACGACGC CTATCGCTCC 
ACATTGAGCT GCATTGTGGT GGACAATGTG GAGCGATTGC TAGACTACGG TCCAATTGGA 
CCCAGGTACT CTAATCTCAC GCTGCAGGCG CTATTGGTTC TACTGAAAAA GCAACCGCCA 
AAGGGTAGAA AACTGCTGAT CCTGTGCACT TCCAGCCGGC GGGACGTTCT CGAGGAGATG 
GAAATGCTCT CGGCTTTCAC GTCCGTCCTG CATGTGTCCA ATCTCTCAAC TCCCGAGAAC 
GTGCTTGCCG TGCTGGATGA TTCTGATTTG TTTAGTCCCG AGGAGCTGCA GAGCATTGCC 
AGGAAAATGG CGGGTAAGCG ATTGTGTATC GGCATCAAGA AGCTGCTGGC TCTCATCGAT 
ATGATCCGGC AATCGGAGCC TCACCAGCGG GTTATCAAGT TCCTCAGCAA AATGGAGGAG 
GAGGGCGGTC TGGAAATGGA TAGGGTGCAA GGGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287319.1
CDS	80..2338
Translation	MSIEKAHRMRAIKCPTDELSLTNKAIVNVSDFTEEVKYVDISPGPGLHYIFALEKISGPELPLGHVGFSLVQRKWATLSINQEIDVRPYRFDASADIITLVSFETDFLQKKTTTQEPYDSDEMAKEFLMQFAGMPLTVGQTLVFQFKDKKFLGLAVKTLEAVDPRTVGDSLPKTRNVRFGRILGNTVVQFEKAENSVLNLQGRSKGKIVRQSIINPDWDFGKMGIGGLDKEFNAIFRRAFASRVFPPELVEQLGIKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANIRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKARGSVAGNSGVHDTVVNQLLAKIDGVEQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMRDFNKIASDVDNNEIAAKTKNFSGAELEGLVRAAQSTAMNRLIKADSKVHVDPEAMEKLRVTRADFLHALDNDIKPAFGAAQEMLENLLARGIINWGPPVTELLEDGMLSVQQAKATESSGLVSVLIEGAPNSGKSALAANLAQLSDFPFVKVCSPEDMVGFTESAKCLHIRKIFDDAYRSTLSCIVVDNVERLLDYGPIGPRYSNLTLQALLVLLKKQPPKGRKLLILCTSSRRDVLEEMEMLSAFTSVLHVSNLSTPENVLAVLDDSDLFSPEELQSIARKMAGKRLCIGIKKLLALIDMIRQSEPHQRVIKFLSKMEEEGGLEMDRVQGH

Target ORF information:

RefSeq Version	NM_001300390.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster N-ethylmaleimide-sensitive factor 2 (Nsf2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300390.1

ORF Insert Sequence:

ATGTCAATTG AAAAAGCCCA CCGGATGAGA GCGATCAAAT GCCCAACGGA TGAGCTGTCC 
CTGACGAACA AGGCGATTGT GAATGTGTCC GATTTCACGG AGGAGGTCAA ATATGTGGAC 
ATCTCGCCGG GCCCGGGGCT GCACTACATC TTCGCGCTGG AGAAGATCAG CGGGCCGGAG 
CTGCCGCTCG GCCACGTCGG ATTCTCGCTG GTCCAGCGCA AGTGGGCCAC GCTCTCCATC 
AACCAGGAAA TAGACGTGCG GCCGTACCGA TTCGATGCCA GCGCCGATAT CATCACACTG 
GTCTCCTTCG AGACGGACTT CCTGCAGAAG AAGACCACCA CGCAGGAGCC CTACGACTCC 
GACGAGATGG CCAAGGAGTT CCTCATGCAG TTCGCCGGAA TGCCGCTGAC CGTCGGCCAA 
ACGCTCGTCT TTCAGTTCAA AGACAAGAAA TTCCTGGGCC TCGCTGTAAA AACGCTGGAG 
GCAGTGGATC CCCGAACAGT GGGCGATTCC CTACCGAAGA CTCGGAACGT ACGATTCGGC 
CGCATCCTCG GTAACACGGT GGTGCAGTTC GAGAAGGCCG AAAACTCTGT GCTAAATCTG 
CAGGGTCGCT CCAAGGGTAA GATCGTGCGG CAGTCCATCA TAAATCCGGA CTGGGACTTT 
GGCAAGATGG GCATTGGTGG CCTAGACAAA GAGTTCAATG CGATTTTCCG ACGCGCCTTC 
GCCTCTCGTG TTTTTCCGCC GGAACTTGTC GAACAACTGG GCATCAAGCA CGTTAAGGGT 
ATTCTGTTGT ACGGACCACC AGGCACGGGA AAAACCCTGA TGGCCCGGCA AATAGGTACC 
ATGCTGAATG CCCGCGAACC CAAGATCGTG AATGGACCGC AGATCCTGGA CAAGTACGTG 
GGTGAATCAG AAGCCAATAT CCGGCGACTG TTTGCCGAGG CCGAGGAGGA GGAAAAGCGC 
TTGGGACCGA ACAGTGGACT GCACATCATC ATCTTCGACG AGATCGACGC CATTTGCAAG 
GCTCGCGGAT CCGTGGCCGG TAATAGTGGT GTCCACGACA CGGTCGTCAA TCAACTGCTA 
GCCAAGATCG ACGGCGTGGA GCAGTTGAAC AACATCCTGG TAATAGGAAT GACCAACCGG 
CGGGACATGA TCGATGAGGC CCTGCTGCGA CCTGGACGCT TGGAAGTGCA AATGGAGATC 
AGCTTGCCCA ACGAGCAGGG TCGCGTCCAG ATCCTGAATA TCCACACCAA GCGGATGCGT 
GACTTTAACA AGATAGCTAG CGATGTGGAC AACAATGAGA TCGCGGCCAA GACAAAGAAC 
TTCAGTGGCG CAGAGTTGGA GGGTCTGGTT AGAGCGGCCC AATCCACGGC CATGAATCGG 
TTAATAAAGG CGGACTCCAA GGTTCACGTG GACCCGGAGG CTATGGAAAA ACTGCGTGTT 
ACGCGAGCCG ACTTTCTGCA CGCATTGGAC AACGACATCA AGCCCGCCTT TGGAGCAGCC 
CAGGAGATGC TGGAAAATCT GCTGGCACGC GGAATTATAA ACTGGGGCCC ACCGGTCACC 
GAACTCCTGG AGGACGGAAT GCTCTCCGTA CAGCAGGCGA AGGCCACTGA GAGCTCTGGC 
CTGGTGTCTG TTCTCATCGA AGGAGCTCCC AACTCCGGCA AATCAGCGCT GGCCGCTAAT 
CTGGCCCAGT TGAGTGATTT CCCATTCGTT AAGGTCTGCT CCCCGGAAGA TATGGTAGGA 
TTTACGGAGT CAGCCAAGTG CCTGCACATC CGCAAGATCT TCGACGACGC CTATCGCTCC 
ACATTGAGCT GCATTGTGGT GGACAATGTG GAGCGATTGC TAGACTACGG TCCAATTGGA 
CCCAGGTACT CTAATCTCAC GCTGCAGGCG CTATTGGTTC TACTGAAAAA GCAACCGCCA 
AAGGGTAGAA AACTGCTGAT CCTGTGCACT TCCAGCCGGC GGGACGTTCT CGAGGAGATG 
GAAATGCTCT CGGCTTTCAC GTCCGTCCTG CATGTGTCCA ATCTCTCAAC TCCCGAGAAC 
GTGCTTGCCG TGCTGGATGA TTCTGATTTG TTTAGTCCCG AGGAGCTGCA GAGCATTGCC 
AGGAAAATGG CGGGTAAGCG ATTGTGTATC GGCATCAAGA AGCTGCTGGC TCTCATCGAT 
ATGATCCGGC AATCGGAGCC TCACCAGCGG GTTATCAAGT TCCTCAGCAA AATGGAGGAG 
GAGGGCGGTC TGGAAATGGA TAGGGTGCAA GGGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsf2 ( NM_001300389.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287318.1 Drosophila melanogaster N-ethylmaleimide-sensitive factor 2 (Nsf2), transcript variant B, mRNA.

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CloneID	OFa08365
Clone ID Related Accession (Same CDS sequence)	NM_176499.2 , NM_001300390.1 , NM_001300389.1
Accession Version	NM_001300389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	N-ethylmaleimide-sensitive factor 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAATTG AAAAAGCCCA CCGGATGAGA GCGATCAAAT GCCCAACGGA TGAGCTGTCC 
CTGACGAACA AGGCGATTGT GAATGTGTCC GATTTCACGG AGGAGGTCAA ATATGTGGAC 
ATCTCGCCGG GCCCGGGGCT GCACTACATC TTCGCGCTGG AGAAGATCAG CGGGCCGGAG 
CTGCCGCTCG GCCACGTCGG ATTCTCGCTG GTCCAGCGCA AGTGGGCCAC GCTCTCCATC 
AACCAGGAAA TAGACGTGCG GCCGTACCGA TTCGATGCCA GCGCCGATAT CATCACACTG 
GTCTCCTTCG AGACGGACTT CCTGCAGAAG AAGACCACCA CGCAGGAGCC CTACGACTCC 
GACGAGATGG CCAAGGAGTT CCTCATGCAG TTCGCCGGAA TGCCGCTGAC CGTCGGCCAA 
ACGCTCGTCT TTCAGTTCAA AGACAAGAAA TTCCTGGGCC TCGCTGTAAA AACGCTGGAG 
GCAGTGGATC CCCGAACAGT GGGCGATTCC CTACCGAAGA CTCGGAACGT ACGATTCGGC 
CGCATCCTCG GTAACACGGT GGTGCAGTTC GAGAAGGCCG AAAACTCTGT GCTAAATCTG 
CAGGGTCGCT CCAAGGGTAA GATCGTGCGG CAGTCCATCA TAAATCCGGA CTGGGACTTT 
GGCAAGATGG GCATTGGTGG CCTAGACAAA GAGTTCAATG CGATTTTCCG ACGCGCCTTC 
GCCTCTCGTG TTTTTCCGCC GGAACTTGTC GAACAACTGG GCATCAAGCA CGTTAAGGGT 
ATTCTGTTGT ACGGACCACC AGGCACGGGA AAAACCCTGA TGGCCCGGCA AATAGGTACC 
ATGCTGAATG CCCGCGAACC CAAGATCGTG AATGGACCGC AGATCCTGGA CAAGTACGTG 
GGTGAATCAG AAGCCAATAT CCGGCGACTG TTTGCCGAGG CCGAGGAGGA GGAAAAGCGC 
TTGGGACCGA ACAGTGGACT GCACATCATC ATCTTCGACG AGATCGACGC CATTTGCAAG 
GCTCGCGGAT CCGTGGCCGG TAATAGTGGT GTCCACGACA CGGTCGTCAA TCAACTGCTA 
GCCAAGATCG ACGGCGTGGA GCAGTTGAAC AACATCCTGG TAATAGGAAT GACCAACCGG 
CGGGACATGA TCGATGAGGC CCTGCTGCGA CCTGGACGCT TGGAAGTGCA AATGGAGATC 
AGCTTGCCCA ACGAGCAGGG TCGCGTCCAG ATCCTGAATA TCCACACCAA GCGGATGCGT 
GACTTTAACA AGATAGCTAG CGATGTGGAC AACAATGAGA TCGCGGCCAA GACAAAGAAC 
TTCAGTGGCG CAGAGTTGGA GGGTCTGGTT AGAGCGGCCC AATCCACGGC CATGAATCGG 
TTAATAAAGG CGGACTCCAA GGTTCACGTG GACCCGGAGG CTATGGAAAA ACTGCGTGTT 
ACGCGAGCCG ACTTTCTGCA CGCATTGGAC AACGACATCA AGCCCGCCTT TGGAGCAGCC 
CAGGAGATGC TGGAAAATCT GCTGGCACGC GGAATTATAA ACTGGGGCCC ACCGGTCACC 
GAACTCCTGG AGGACGGAAT GCTCTCCGTA CAGCAGGCGA AGGCCACTGA GAGCTCTGGC 
CTGGTGTCTG TTCTCATCGA AGGAGCTCCC AACTCCGGCA AATCAGCGCT GGCCGCTAAT 
CTGGCCCAGT TGAGTGATTT CCCATTCGTT AAGGTCTGCT CCCCGGAAGA TATGGTAGGA 
TTTACGGAGT CAGCCAAGTG CCTGCACATC CGCAAGATCT TCGACGACGC CTATCGCTCC 
ACATTGAGCT GCATTGTGGT GGACAATGTG GAGCGATTGC TAGACTACGG TCCAATTGGA 
CCCAGGTACT CTAATCTCAC GCTGCAGGCG CTATTGGTTC TACTGAAAAA GCAACCGCCA 
AAGGGTAGAA AACTGCTGAT CCTGTGCACT TCCAGCCGGC GGGACGTTCT CGAGGAGATG 
GAAATGCTCT CGGCTTTCAC GTCCGTCCTG CATGTGTCCA ATCTCTCAAC TCCCGAGAAC 
GTGCTTGCCG TGCTGGATGA TTCTGATTTG TTTAGTCCCG AGGAGCTGCA GAGCATTGCC 
AGGAAAATGG CGGGTAAGCG ATTGTGTATC GGCATCAAGA AGCTGCTGGC TCTCATCGAT 
ATGATCCGGC AATCGGAGCC TCACCAGCGG GTTATCAAGT TCCTCAGCAA AATGGAGGAG 
GAGGGCGGTC TGGAAATGGA TAGGGTGCAA GGGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287318.1
CDS	80..2338
Translation	MSIEKAHRMRAIKCPTDELSLTNKAIVNVSDFTEEVKYVDISPGPGLHYIFALEKISGPELPLGHVGFSLVQRKWATLSINQEIDVRPYRFDASADIITLVSFETDFLQKKTTTQEPYDSDEMAKEFLMQFAGMPLTVGQTLVFQFKDKKFLGLAVKTLEAVDPRTVGDSLPKTRNVRFGRILGNTVVQFEKAENSVLNLQGRSKGKIVRQSIINPDWDFGKMGIGGLDKEFNAIFRRAFASRVFPPELVEQLGIKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANIRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKARGSVAGNSGVHDTVVNQLLAKIDGVEQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMRDFNKIASDVDNNEIAAKTKNFSGAELEGLVRAAQSTAMNRLIKADSKVHVDPEAMEKLRVTRADFLHALDNDIKPAFGAAQEMLENLLARGIINWGPPVTELLEDGMLSVQQAKATESSGLVSVLIEGAPNSGKSALAANLAQLSDFPFVKVCSPEDMVGFTESAKCLHIRKIFDDAYRSTLSCIVVDNVERLLDYGPIGPRYSNLTLQALLVLLKKQPPKGRKLLILCTSSRRDVLEEMEMLSAFTSVLHVSNLSTPENVLAVLDDSDLFSPEELQSIARKMAGKRLCIGIKKLLALIDMIRQSEPHQRVIKFLSKMEEEGGLEMDRVQGH

Target ORF information:

RefSeq Version	NM_001300389.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster N-ethylmaleimide-sensitive factor 2 (Nsf2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300389.1

ORF Insert Sequence:

ATGTCAATTG AAAAAGCCCA CCGGATGAGA GCGATCAAAT GCCCAACGGA TGAGCTGTCC 
CTGACGAACA AGGCGATTGT GAATGTGTCC GATTTCACGG AGGAGGTCAA ATATGTGGAC 
ATCTCGCCGG GCCCGGGGCT GCACTACATC TTCGCGCTGG AGAAGATCAG CGGGCCGGAG 
CTGCCGCTCG GCCACGTCGG ATTCTCGCTG GTCCAGCGCA AGTGGGCCAC GCTCTCCATC 
AACCAGGAAA TAGACGTGCG GCCGTACCGA TTCGATGCCA GCGCCGATAT CATCACACTG 
GTCTCCTTCG AGACGGACTT CCTGCAGAAG AAGACCACCA CGCAGGAGCC CTACGACTCC 
GACGAGATGG CCAAGGAGTT CCTCATGCAG TTCGCCGGAA TGCCGCTGAC CGTCGGCCAA 
ACGCTCGTCT TTCAGTTCAA AGACAAGAAA TTCCTGGGCC TCGCTGTAAA AACGCTGGAG 
GCAGTGGATC CCCGAACAGT GGGCGATTCC CTACCGAAGA CTCGGAACGT ACGATTCGGC 
CGCATCCTCG GTAACACGGT GGTGCAGTTC GAGAAGGCCG AAAACTCTGT GCTAAATCTG 
CAGGGTCGCT CCAAGGGTAA GATCGTGCGG CAGTCCATCA TAAATCCGGA CTGGGACTTT 
GGCAAGATGG GCATTGGTGG CCTAGACAAA GAGTTCAATG CGATTTTCCG ACGCGCCTTC 
GCCTCTCGTG TTTTTCCGCC GGAACTTGTC GAACAACTGG GCATCAAGCA CGTTAAGGGT 
ATTCTGTTGT ACGGACCACC AGGCACGGGA AAAACCCTGA TGGCCCGGCA AATAGGTACC 
ATGCTGAATG CCCGCGAACC CAAGATCGTG AATGGACCGC AGATCCTGGA CAAGTACGTG 
GGTGAATCAG AAGCCAATAT CCGGCGACTG TTTGCCGAGG CCGAGGAGGA GGAAAAGCGC 
TTGGGACCGA ACAGTGGACT GCACATCATC ATCTTCGACG AGATCGACGC CATTTGCAAG 
GCTCGCGGAT CCGTGGCCGG TAATAGTGGT GTCCACGACA CGGTCGTCAA TCAACTGCTA 
GCCAAGATCG ACGGCGTGGA GCAGTTGAAC AACATCCTGG TAATAGGAAT GACCAACCGG 
CGGGACATGA TCGATGAGGC CCTGCTGCGA CCTGGACGCT TGGAAGTGCA AATGGAGATC 
AGCTTGCCCA ACGAGCAGGG TCGCGTCCAG ATCCTGAATA TCCACACCAA GCGGATGCGT 
GACTTTAACA AGATAGCTAG CGATGTGGAC AACAATGAGA TCGCGGCCAA GACAAAGAAC 
TTCAGTGGCG CAGAGTTGGA GGGTCTGGTT AGAGCGGCCC AATCCACGGC CATGAATCGG 
TTAATAAAGG CGGACTCCAA GGTTCACGTG GACCCGGAGG CTATGGAAAA ACTGCGTGTT 
ACGCGAGCCG ACTTTCTGCA CGCATTGGAC AACGACATCA AGCCCGCCTT TGGAGCAGCC 
CAGGAGATGC TGGAAAATCT GCTGGCACGC GGAATTATAA ACTGGGGCCC ACCGGTCACC 
GAACTCCTGG AGGACGGAAT GCTCTCCGTA CAGCAGGCGA AGGCCACTGA GAGCTCTGGC 
CTGGTGTCTG TTCTCATCGA AGGAGCTCCC AACTCCGGCA AATCAGCGCT GGCCGCTAAT 
CTGGCCCAGT TGAGTGATTT CCCATTCGTT AAGGTCTGCT CCCCGGAAGA TATGGTAGGA 
TTTACGGAGT CAGCCAAGTG CCTGCACATC CGCAAGATCT TCGACGACGC CTATCGCTCC 
ACATTGAGCT GCATTGTGGT GGACAATGTG GAGCGATTGC TAGACTACGG TCCAATTGGA 
CCCAGGTACT CTAATCTCAC GCTGCAGGCG CTATTGGTTC TACTGAAAAA GCAACCGCCA 
AAGGGTAGAA AACTGCTGAT CCTGTGCACT TCCAGCCGGC GGGACGTTCT CGAGGAGATG 
GAAATGCTCT CGGCTTTCAC GTCCGTCCTG CATGTGTCCA ATCTCTCAAC TCCCGAGAAC 
GTGCTTGCCG TGCTGGATGA TTCTGATTTG TTTAGTCCCG AGGAGCTGCA GAGCATTGCC 
AGGAAAATGG CGGGTAAGCG ATTGTGTATC GGCATCAAGA AGCTGCTGGC TCTCATCGAT 
ATGATCCGGC AATCGGAGCC TCACCAGCGG GTTATCAAGT TCCTCAGCAA AATGGAGGAG 
GAGGGCGGTC TGGAAATGGA TAGGGTGCAA GGGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nsf2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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