sqd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sqd
Entrez Gene ID	41666
Full Name	squid
Synonyms	BcDNA:GM03761,CG16901,CG17791,CR33328,Dmel\CG16901,Dmel_CR33328,HRP40,Hrb87,Hrb87Fb,Hrp40,RRM3,Rbp3,SQD,Sqd,Squid,fs(3)00048,hnRNP 1,hnRNP A,hrp40,l(3)EP3631,l(3)j4B4,l(3)j6E3,l(3)j8E7,rrm3
General protein information	Preferred Names squid Names CG16901 gene product from transcript CG16901-RE CG16901-PA CG16901-PB CG16901-PC CG16901-PD CG16901-PE RNA-binding protein 3 heterogeneous-nuclear-ribonucleoprotein-87Fb lethal (3) j4B4 sqd-PA sqd-PB sqd-PC sqd-PD sqd-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87F5-87F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_169528.2	NP_731825.1	squid, isoform B
NM_169529.3	NP_731826.1	squid, isoform A
NM_206481.2	NP_996203.1	squid, isoform D
NM_001260159.2	NP_001247088.1	squid, isoform E
NM_143952.2	NP_652209.1	squid, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	sqd	NP_001247088.1
Bos taurus (cattle)	HNRNPD	NP_001091533.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rbf physically and genetically interacts with Sqd.
Title: Sqd interacts with the Drosophila retinoblastoma tumor suppressor Rbf.

Data suggest that that Squid interacts with gurken mRNA in the nurse cell nuclei and, together with other factors, maintains gurken in a translationally silent state during its transport to the dorsal anterior region of the oocyte.
Title: Translational repression of gurken mRNA in the Drosophila oocyte requires the hnRNP Squid in the nurse cells.

sqd, cup, and PABP55B function together to regulate gurken translation.
Title: Squid, Cup, and PABP55B function together to regulate gurken translation in Drosophila.

Squid is required for the dynamic conversion of transport particles to sponge bodies.
Title: Drosophila Squid/hnRNP helps Dynein switch from a gurken mRNA transport motor to an ultrastructural static anchor in sponge bodies.

Loss of Sqd does not affect polarity in follicle cells, wings or eyes, indicating a specific role in the determination of MT polarity within the germline.
Title: The RNA-binding protein Squid is required for the establishment of anteroposterior polarity in the Drosophila oocyte.

The following sqd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sqd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04817	NM_143952.2 Latest version!	Drosophila melanogaster squid (sqd), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa04817	NM_001260159.2 Latest version!	Drosophila melanogaster squid (sqd), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08340	NM_169529.3 Latest version!	Drosophila melanogaster squid (sqd), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08379	NM_169528.2 Latest version!	Drosophila melanogaster squid (sqd), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08384	NM_206481.2 Latest version!	Drosophila melanogaster squid (sqd), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sqd ( NM_001260159.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247088.1 Drosophila melanogaster squid (sqd), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa04817
Clone ID Related Accession (Same CDS sequence)	NM_001260159.2 , NM_143952.2
Accession Version	NM_001260159.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	squid, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260159.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGA ACAAGCAAGT GGATACAGAA ATAAACGGCG AGGATTTCAC CAAGGACGTG 
ACTGCCGACG GTCCGGGATC TGAAAATGGT GATGCGGGCG CCGCCGGCTC GACCAACGGC 
AGTTCGGACA ACCAATCGGC CGCATCCGGC CAGCGGGACG ACGACAGGAA ACTGTTTGTC 
GGTGGTCTGA GCTGGGAAAC GACTGAGAAG GAACTCCGCG ATCACTTCGG CAAATATGGC 
GAGATCGAGA GCATCAATGT CAAGACAGAT CCCCAGACCG GTCGGTCCCG AGGATTCGCC 
TTCATCGTGT TTACAAACAC CGAGGCCATT GACAAAGTCA GCGCCGCGGA TGAGCACATA 
ATCAACAGCA AGAAGGTCGA TCCCAAGAAG GCCAAGGCCA GGCACGGCAA GATCTTTGTC 
GGCGGCCTCA CCACAGAGAT CAGCGATGAG GAGATTAAGA CCTACTTTGG ACAGTTCGGC 
AATATCGTCG AGGTGGAGAT GCCATTCGAC AAGCAAAAGT CGCAGCGCAA AGGATTCTGC 
TTCATCACCT TCGATTCGGA GCAGGTGGTG ACAGATTTGC TGAAGACGCC CAAGCAGAAG 
ATCGCCGGCA AGGAGGTCGA TGTTAAGCGT GCGACGCCCA AGCCGGAGAA CCAGATGATG 
GGCGGTATGC GTGGTGGACC ACGCGGTGGT ATGCGCGGCG GACGCGGTGG CTACGGAGGA 
CGAGGTGGCT ACAACAACCA GTGGGACGGA CAGGGATCAT ACGGCGGCTA TGGCGGCGGC 
TACGGCGGAT ATGGTGCCGG TGGTTATGGC GACTACTATG CCGGCGGCTA CTATAATGGA 
TATGACTACG GTTATGGCGG TGGCGGTTTT AACGGTGGCA AGCAGCGCGG AGGCGGTGGA 
CGCCAGCAGC GGCATCAGCC CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247088.1
CDS	386..1312
Translation	MAENKQVDTEINGEDFTKDVTADGPGSENGDAGAAGSTNGSSDNQSAASGQRDDDRKLFVGGLSWETTEKELRDHFGKYGEIESINVKTDPQTGRSRGFAFIVFTNTEAIDKVSAADEHIINSKKVDPKKAKARHGKIFVGGLTTEISDEEIKTYFGQFGNIVEVEMPFDKQKSQRKGFCFITFDSEQVVTDLLKTPKQKIAGKEVDVKRATPKPENQMMGGMRGGPRGGMRGGRGGYGGRGGYNNQWDGQGSYGGYGGGYGGYGAGGYGDYYAGGYYNGYDYGYGGGGFNGGKQRGGGGRQQRHQPY

Target ORF information:

RefSeq Version	NM_001260159.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster squid (sqd), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260159.2

ORF Insert Sequence:

ATGGCCGAGA ACAAGCAAGT GGATACAGAA ATAAACGGCG AGGATTTCAC CAAGGACGTG 
ACTGCCGACG GTCCGGGATC TGAAAATGGT GATGCGGGCG CCGCCGGCTC GACCAACGGC 
AGTTCGGACA ACCAATCGGC CGCATCCGGC CAGCGGGACG ACGACAGGAA ACTGTTTGTC 
GGTGGTCTGA GCTGGGAAAC GACTGAGAAG GAACTCCGCG ATCACTTCGG CAAATATGGC 
GAGATCGAGA GCATCAATGT CAAGACAGAT CCCCAGACCG GTCGGTCCCG AGGATTCGCC 
TTCATCGTGT TTACAAACAC CGAGGCCATT GACAAAGTCA GCGCCGCGGA TGAGCACATA 
ATCAACAGCA AGAAGGTCGA TCCCAAGAAG GCCAAGGCCA GGCACGGCAA GATCTTTGTC 
GGCGGCCTCA CCACAGAGAT CAGCGATGAG GAGATTAAGA CCTACTTTGG ACAGTTCGGC 
AATATCGTCG AGGTGGAGAT GCCATTCGAC AAGCAAAAGT CGCAGCGCAA AGGATTCTGC 
TTCATCACCT TCGATTCGGA GCAGGTGGTG ACAGATTTGC TGAAGACGCC CAAGCAGAAG 
ATCGCCGGCA AGGAGGTCGA TGTTAAGCGT GCGACGCCCA AGCCGGAGAA CCAGATGATG 
GGCGGTATGC GTGGTGGACC ACGCGGTGGT ATGCGCGGCG GACGCGGTGG CTACGGAGGA 
CGAGGTGGCT ACAACAACCA GTGGGACGGA CAGGGATCAT ACGGCGGCTA TGGCGGCGGC 
TACGGCGGAT ATGGTGCCGG TGGTTATGGC GACTACTATG CCGGCGGCTA CTATAATGGA 
TATGACTACG GTTATGGCGG TGGCGGTTTT AACGGTGGCA AGCAGCGCGG AGGCGGTGGA 
CGCCAGCAGC GGCATCAGCC CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqd ( NM_143952.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652209.1 Drosophila melanogaster squid (sqd), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04817
Clone ID Related Accession (Same CDS sequence)	NM_001260159.2 , NM_143952.2
Accession Version	NM_143952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	squid, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_143952.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGA ACAAGCAAGT GGATACAGAA ATAAACGGCG AGGATTTCAC CAAGGACGTG 
ACTGCCGACG GTCCGGGATC TGAAAATGGT GATGCGGGCG CCGCCGGCTC GACCAACGGC 
AGTTCGGACA ACCAATCGGC CGCATCCGGC CAGCGGGACG ACGACAGGAA ACTGTTTGTC 
GGTGGTCTGA GCTGGGAAAC GACTGAGAAG GAACTCCGCG ATCACTTCGG CAAATATGGC 
GAGATCGAGA GCATCAATGT CAAGACAGAT CCCCAGACCG GTCGGTCCCG AGGATTCGCC 
TTCATCGTGT TTACAAACAC CGAGGCCATT GACAAAGTCA GCGCCGCGGA TGAGCACATA 
ATCAACAGCA AGAAGGTCGA TCCCAAGAAG GCCAAGGCCA GGCACGGCAA GATCTTTGTC 
GGCGGCCTCA CCACAGAGAT CAGCGATGAG GAGATTAAGA CCTACTTTGG ACAGTTCGGC 
AATATCGTCG AGGTGGAGAT GCCATTCGAC AAGCAAAAGT CGCAGCGCAA AGGATTCTGC 
TTCATCACCT TCGATTCGGA GCAGGTGGTG ACAGATTTGC TGAAGACGCC CAAGCAGAAG 
ATCGCCGGCA AGGAGGTCGA TGTTAAGCGT GCGACGCCCA AGCCGGAGAA CCAGATGATG 
GGCGGTATGC GTGGTGGACC ACGCGGTGGT ATGCGCGGCG GACGCGGTGG CTACGGAGGA 
CGAGGTGGCT ACAACAACCA GTGGGACGGA CAGGGATCAT ACGGCGGCTA TGGCGGCGGC 
TACGGCGGAT ATGGTGCCGG TGGTTATGGC GACTACTATG CCGGCGGCTA CTATAATGGA 
TATGACTACG GTTATGGCGG TGGCGGTTTT AACGGTGGCA AGCAGCGCGG AGGCGGTGGA 
CGCCAGCAGC GGCATCAGCC CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652209.1
CDS	111..1037
Translation	MAENKQVDTEINGEDFTKDVTADGPGSENGDAGAAGSTNGSSDNQSAASGQRDDDRKLFVGGLSWETTEKELRDHFGKYGEIESINVKTDPQTGRSRGFAFIVFTNTEAIDKVSAADEHIINSKKVDPKKAKARHGKIFVGGLTTEISDEEIKTYFGQFGNIVEVEMPFDKQKSQRKGFCFITFDSEQVVTDLLKTPKQKIAGKEVDVKRATPKPENQMMGGMRGGPRGGMRGGRGGYGGRGGYNNQWDGQGSYGGYGGGYGGYGAGGYGDYYAGGYYNGYDYGYGGGGFNGGKQRGGGGRQQRHQPY

Target ORF information:

RefSeq Version	NM_143952.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster squid (sqd), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143952.2

ORF Insert Sequence:

ATGGCCGAGA ACAAGCAAGT GGATACAGAA ATAAACGGCG AGGATTTCAC CAAGGACGTG 
ACTGCCGACG GTCCGGGATC TGAAAATGGT GATGCGGGCG CCGCCGGCTC GACCAACGGC 
AGTTCGGACA ACCAATCGGC CGCATCCGGC CAGCGGGACG ACGACAGGAA ACTGTTTGTC 
GGTGGTCTGA GCTGGGAAAC GACTGAGAAG GAACTCCGCG ATCACTTCGG CAAATATGGC 
GAGATCGAGA GCATCAATGT CAAGACAGAT CCCCAGACCG GTCGGTCCCG AGGATTCGCC 
TTCATCGTGT TTACAAACAC CGAGGCCATT GACAAAGTCA GCGCCGCGGA TGAGCACATA 
ATCAACAGCA AGAAGGTCGA TCCCAAGAAG GCCAAGGCCA GGCACGGCAA GATCTTTGTC 
GGCGGCCTCA CCACAGAGAT CAGCGATGAG GAGATTAAGA CCTACTTTGG ACAGTTCGGC 
AATATCGTCG AGGTGGAGAT GCCATTCGAC AAGCAAAAGT CGCAGCGCAA AGGATTCTGC 
TTCATCACCT TCGATTCGGA GCAGGTGGTG ACAGATTTGC TGAAGACGCC CAAGCAGAAG 
ATCGCCGGCA AGGAGGTCGA TGTTAAGCGT GCGACGCCCA AGCCGGAGAA CCAGATGATG 
GGCGGTATGC GTGGTGGACC ACGCGGTGGT ATGCGCGGCG GACGCGGTGG CTACGGAGGA 
CGAGGTGGCT ACAACAACCA GTGGGACGGA CAGGGATCAT ACGGCGGCTA TGGCGGCGGC 
TACGGCGGAT ATGGTGCCGG TGGTTATGGC GACTACTATG CCGGCGGCTA CTATAATGGA 
TATGACTACG GTTATGGCGG TGGCGGTTTT AACGGTGGCA AGCAGCGCGG AGGCGGTGGA 
CGCCAGCAGC GGCATCAGCC CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqd ( NM_169529.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731826.1 Drosophila melanogaster squid (sqd), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa08340
Clone ID Related Accession (Same CDS sequence)	NM_169529.3
Accession Version	NM_169529.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	squid, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_169529.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGA ACAAGCAAGT GGATACAGAA ATAAACGGCG AGGATTTCAC CAAGGACGTG 
ACTGCCGACG GTCCGGGATC TGAAAATGGT GATGCGGGCG CCGCCGGCTC GACCAACGGC 
AGTTCGGACA ACCAATCGGC CGCATCCGGC CAGCGGGACG ACGACAGGAA ACTGTTTGTC 
GGTGGTCTGA GCTGGGAAAC GACTGAGAAG GAACTCCGCG ATCACTTCGG CAAATATGGC 
GAGATCGAGA GCATCAATGT CAAGACAGAT CCCCAGACCG GTCGGTCCCG AGGATTCGCC 
TTCATCGTGT TTACAAACAC CGAGGCCATT GACAAAGTCA GCGCCGCGGA TGAGCACATA 
ATCAACAGCA AGAAGGTCGA TCCCAAGAAG GCCAAGGCCA GGCACGGCAA GATCTTTGTC 
GGCGGCCTCA CCACAGAGAT CAGCGATGAG GAGATTAAGA CCTACTTTGG ACAGTTCGGC 
AATATCGTCG AGGTGGAGAT GCCATTCGAC AAGCAAAAGT CGCAGCGCAA AGGATTCTGC 
TTCATCACCT TCGATTCGGA GCAGGTGGTG ACAGATTTGC TGAAGACGCC CAAGCAGAAG 
ATCGCCGGCA AGGAGGTCGA TGTTAAGCGT GCGACGCCCA AGCCGGAGAA CCAGATGATG 
GGCGGTATGC GTGGTGGACC ACGCGGTGGT ATGCGCGGCG GACGCGGTGG CTACGGAGGA 
CGAGGTGGCT ACAACAACCA GTGGGACGGA CAGGGATCAT ACGGCGGCTA TGGCGGCGGC 
TACGGCGGAT ATGGTGCCGG TGGTTATGGC GACTACTATG CCGGCGGCTA CTATAATGGA 
TATGACTACG GTTATGGTAA ATACAACAAA CAACAAAGTA GTGCTCAAAA CAATTATTAC 
AATAACAACA CGTCGAGTAA TTACCATCAA AACAAGAACA ATAGCAACAA CTATCAGCAG 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731826.1
CDS	115..1080
Translation	MAENKQVDTEINGEDFTKDVTADGPGSENGDAGAAGSTNGSSDNQSAASGQRDDDRKLFVGGLSWETTEKELRDHFGKYGEIESINVKTDPQTGRSRGFAFIVFTNTEAIDKVSAADEHIINSKKVDPKKAKARHGKIFVGGLTTEISDEEIKTYFGQFGNIVEVEMPFDKQKSQRKGFCFITFDSEQVVTDLLKTPKQKIAGKEVDVKRATPKPENQMMGGMRGGPRGGMRGGRGGYGGRGGYNNQWDGQGSYGGYGGGYGGYGAGGYGDYYAGGYYNGYDYGYGKYNKQQSSAQNNYYNNNTSSNYHQNKNNSNNYQQF

Target ORF information:

RefSeq Version	NM_169529.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster squid (sqd), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169529.3

ORF Insert Sequence:

ATGGCCGAGA ACAAGCAAGT GGATACAGAA ATAAACGGCG AGGATTTCAC CAAGGACGTG 
ACTGCCGACG GTCCGGGATC TGAAAATGGT GATGCGGGCG CCGCCGGCTC GACCAACGGC 
AGTTCGGACA ACCAATCGGC CGCATCCGGC CAGCGGGACG ACGACAGGAA ACTGTTTGTC 
GGTGGTCTGA GCTGGGAAAC GACTGAGAAG GAACTCCGCG ATCACTTCGG CAAATATGGC 
GAGATCGAGA GCATCAATGT CAAGACAGAT CCCCAGACCG GTCGGTCCCG AGGATTCGCC 
TTCATCGTGT TTACAAACAC CGAGGCCATT GACAAAGTCA GCGCCGCGGA TGAGCACATA 
ATCAACAGCA AGAAGGTCGA TCCCAAGAAG GCCAAGGCCA GGCACGGCAA GATCTTTGTC 
GGCGGCCTCA CCACAGAGAT CAGCGATGAG GAGATTAAGA CCTACTTTGG ACAGTTCGGC 
AATATCGTCG AGGTGGAGAT GCCATTCGAC AAGCAAAAGT CGCAGCGCAA AGGATTCTGC 
TTCATCACCT TCGATTCGGA GCAGGTGGTG ACAGATTTGC TGAAGACGCC CAAGCAGAAG 
ATCGCCGGCA AGGAGGTCGA TGTTAAGCGT GCGACGCCCA AGCCGGAGAA CCAGATGATG 
GGCGGTATGC GTGGTGGACC ACGCGGTGGT ATGCGCGGCG GACGCGGTGG CTACGGAGGA 
CGAGGTGGCT ACAACAACCA GTGGGACGGA CAGGGATCAT ACGGCGGCTA TGGCGGCGGC 
TACGGCGGAT ATGGTGCCGG TGGTTATGGC GACTACTATG CCGGCGGCTA CTATAATGGA 
TATGACTACG GTTATGGTAA ATACAACAAA CAACAAAGTA GTGCTCAAAA CAATTATTAC 
AATAACAACA CGTCGAGTAA TTACCATCAA AACAAGAACA ATAGCAACAA CTATCAGCAG 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqd ( NM_169528.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731825.1 Drosophila melanogaster squid (sqd), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08379
Clone ID Related Accession (Same CDS sequence)	NM_169528.2
Accession Version	NM_169528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	squid, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_169528.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGA ACAAGCAAGT GGATACAGAA ATAAACGGCG AGGATTTCAC CAAGGACGTG 
ACTGCCGACG GTCCGGGATC TGAAAATGGT GATGCGGGCG CCGCCGGCTC GACCAACGGC 
AGTTCGGACA ACCAATCGGC CGCATCCGGC CAGCGGGACG ACGACAGGAA ACTGTTTGTC 
GGTGGTCTGA GCTGGGAAAC GACTGAGAAG GAACTCCGCG ATCACTTCGG CAAATATGGC 
GAGATCGAGA GCATCAATGT CAAGACAGAT CCCCAGACCG GTCGGTCCCG AGGATTCGCC 
TTCATCGTGT TTACAAACAC CGAGGCCATT GACAAAGTCA GCGCCGCGGA TGAGCACATA 
ATCAACAGCA AGAAGGTCGA TCCCAAGAAG GCCAAGGCCA GGCACGGCAA GATCTTTGTC 
GGCGGCCTCA CCACAGAGAT CAGCGATGAG GAGATTAAGA CCTACTTTGG ACAGTTCGGC 
AATATCGTCG AGGTGGAGAT GCCATTCGAC AAGCAAAAGT CGCAGCGCAA AGGATTCTGC 
TTCATCACCT TCGATTCGGA GCAGGTGGTG ACAGATTTGC TGAAGACGCC CAAGCAGAAG 
ATCGCCGGCA AGGAGGTCGA TGTTAAGCGT GCGACGCCCA AGCCGGAGAA CCAGATGATG 
GGCGGTATGC GTGGTGGACC ACGCGGTGGT ATGCGCGGCG GACGCGGTGG CTACGGAGGA 
CGAGGTGGCT ACAACAACCA GTGGGACGGA CAGGGATCAT ACGGCGGCTA TGGCGGCGGC 
TACGGCGGAT ATGGTGCCGG TGGTTATGGC GACTACTATG CCGGCGGCTA CTATAATGGA 
TATGACTACG GTTATGACGG CTACGGTTAC GGCGGCGGCT TTGAGGGTAA CGGCTACGGC 
GGAGGCGGTG GTGGCAATAT GGGCGGCGGT CGCGGTGGAC CCCGCGGCGG CGGCGGCCCC 
AAAGGCGGTG GCGGTTTTAA CGGTGGCAAG CAGCGCGGAG GCGGTGGACG CCAGCAGCGG 
CATCAGCCCT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731825.1
CDS	386..1420
Translation	MAENKQVDTEINGEDFTKDVTADGPGSENGDAGAAGSTNGSSDNQSAASGQRDDDRKLFVGGLSWETTEKELRDHFGKYGEIESINVKTDPQTGRSRGFAFIVFTNTEAIDKVSAADEHIINSKKVDPKKAKARHGKIFVGGLTTEISDEEIKTYFGQFGNIVEVEMPFDKQKSQRKGFCFITFDSEQVVTDLLKTPKQKIAGKEVDVKRATPKPENQMMGGMRGGPRGGMRGGRGGYGGRGGYNNQWDGQGSYGGYGGGYGGYGAGGYGDYYAGGYYNGYDYGYDGYGYGGGFEGNGYGGGGGGNMGGGRGGPRGGGGPKGGGGFNGGKQRGGGGRQQRHQPY

Target ORF information:

RefSeq Version	NM_169528.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster squid (sqd), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169528.2

ORF Insert Sequence:

ATGGCCGAGA ACAAGCAAGT GGATACAGAA ATAAACGGCG AGGATTTCAC CAAGGACGTG 
ACTGCCGACG GTCCGGGATC TGAAAATGGT GATGCGGGCG CCGCCGGCTC GACCAACGGC 
AGTTCGGACA ACCAATCGGC CGCATCCGGC CAGCGGGACG ACGACAGGAA ACTGTTTGTC 
GGTGGTCTGA GCTGGGAAAC GACTGAGAAG GAACTCCGCG ATCACTTCGG CAAATATGGC 
GAGATCGAGA GCATCAATGT CAAGACAGAT CCCCAGACCG GTCGGTCCCG AGGATTCGCC 
TTCATCGTGT TTACAAACAC CGAGGCCATT GACAAAGTCA GCGCCGCGGA TGAGCACATA 
ATCAACAGCA AGAAGGTCGA TCCCAAGAAG GCCAAGGCCA GGCACGGCAA GATCTTTGTC 
GGCGGCCTCA CCACAGAGAT CAGCGATGAG GAGATTAAGA CCTACTTTGG ACAGTTCGGC 
AATATCGTCG AGGTGGAGAT GCCATTCGAC AAGCAAAAGT CGCAGCGCAA AGGATTCTGC 
TTCATCACCT TCGATTCGGA GCAGGTGGTG ACAGATTTGC TGAAGACGCC CAAGCAGAAG 
ATCGCCGGCA AGGAGGTCGA TGTTAAGCGT GCGACGCCCA AGCCGGAGAA CCAGATGATG 
GGCGGTATGC GTGGTGGACC ACGCGGTGGT ATGCGCGGCG GACGCGGTGG CTACGGAGGA 
CGAGGTGGCT ACAACAACCA GTGGGACGGA CAGGGATCAT ACGGCGGCTA TGGCGGCGGC 
TACGGCGGAT ATGGTGCCGG TGGTTATGGC GACTACTATG CCGGCGGCTA CTATAATGGA 
TATGACTACG GTTATGACGG CTACGGTTAC GGCGGCGGCT TTGAGGGTAA CGGCTACGGC 
GGAGGCGGTG GTGGCAATAT GGGCGGCGGT CGCGGTGGAC CCCGCGGCGG CGGCGGCCCC 
AAAGGCGGTG GCGGTTTTAA CGGTGGCAAG CAGCGCGGAG GCGGTGGACG CCAGCAGCGG 
CATCAGCCCT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqd ( NM_206481.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996203.1 Drosophila melanogaster squid (sqd), transcript variant D, mRNA.

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CloneID	OFa08384
Clone ID Related Accession (Same CDS sequence)	NM_206481.2
Accession Version	NM_206481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	squid, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_206481.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCATTCG ACAAGCAAAA GTCGCAGCGC AAAGGATTCT GCTTCATCAC CTTCGATTCG 
GAGCAGGTGG TGACAGATTT GCTGAAGACG CCCAAGCAGA AGATCGCCGG CAAGGAGGTC 
GATGTTAAGC GTGCGACGCC CAAGCCGGAG AACCAGATGA TGGGCGGTAT GCGTGGTGGA 
CCACGCGGTG GTATGCGCGG CGGACGCGGT GGCTACGGAG GACGAGGTGG CTACAACAAC 
CAGTGGGACG GACAGGGATC ATACGGCGGC TATGGCGGCG GCTACGGCGG ATATGGTGCC 
GGTGGTTATG GCGACTACTA TGCCGGCGGC TACTATAATG GATATGACTA CGGTTATGAC 
GGCTACGGTT ACGGCGGCGG CTTTGAGGGT AACGGCTACG GCGGAGGCGG TGGTGGCAAT 
ATGGGCGGCG GTCGCGGTGG ACCCCGCGGC GGCGGCGGCC CCAAAGGCGG TGGCGGTTTT 
AACGGTGGCA AGCAGCGCGG AGGCGGTGGA CGCCAGCAGC GGCATCAGCC CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996203.1
CDS	1096..1632
Translation	MPFDKQKSQRKGFCFITFDSEQVVTDLLKTPKQKIAGKEVDVKRATPKPENQMMGGMRGGPRGGMRGGRGGYGGRGGYNNQWDGQGSYGGYGGGYGGYGAGGYGDYYAGGYYNGYDYGYDGYGYGGGFEGNGYGGGGGGNMGGGRGGPRGGGGPKGGGGFNGGKQRGGGGRQQRHQPY

Target ORF information:

RefSeq Version	NM_206481.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster squid (sqd), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206481.2

ORF Insert Sequence:

ATGCCATTCG ACAAGCAAAA GTCGCAGCGC AAAGGATTCT GCTTCATCAC CTTCGATTCG 
GAGCAGGTGG TGACAGATTT GCTGAAGACG CCCAAGCAGA AGATCGCCGG CAAGGAGGTC 
GATGTTAAGC GTGCGACGCC CAAGCCGGAG AACCAGATGA TGGGCGGTAT GCGTGGTGGA 
CCACGCGGTG GTATGCGCGG CGGACGCGGT GGCTACGGAG GACGAGGTGG CTACAACAAC 
CAGTGGGACG GACAGGGATC ATACGGCGGC TATGGCGGCG GCTACGGCGG ATATGGTGCC 
GGTGGTTATG GCGACTACTA TGCCGGCGGC TACTATAATG GATATGACTA CGGTTATGAC 
GGCTACGGTT ACGGCGGCGG CTTTGAGGGT AACGGCTACG GCGGAGGCGG TGGTGGCAAT 
ATGGGCGGCG GTCGCGGTGG ACCCCGCGGC GGCGGCGGCC CCAAAGGCGG TGGCGGTTTT 
AACGGTGGCA AGCAGCGCGG AGGCGGTGGA CGCCAGCAGC GGCATCAGCC CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sqd cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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