Ilp8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ilp8
Entrez Gene ID	39909
Full Name	Insulin-like peptide 8
Synonyms	CG14059,DILP8,Dilp8,Dmel\CG14059,dILP-8,dILP8,dilp8,dlip8
General protein information	Preferred Names Insulin-like peptide 8 Names CG14059 gene product from transcript CG14059-RB CG14059-PB Ilp8-PB drosophila insulin-like peptide 8
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 73E4-73E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_140692.3	NP_648949.2	Insulin-like peptide 8

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here we demonstrate that the Drosophila relaxin receptor homolog Lgr3, a leucine-rich repeat-containing G-protein-coupled receptor, is required for Dilp8-dependent growth coordination and developmental delay during the regeneration checkpoint; we demonstrate that Lgr3 activity in both the CNS and PG is necessary for NOS activation in the PG following damage
Title: Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration Is Mediated by Signaling Through the Relaxin Receptor Lgr3 in the Prothoracic Gland.

this study shows Lgr3 is a Dilp8 receptor
Title: A brain circuit that synchronizes growth and maturation revealed through Dilp8 binding to Lgr3.

Mutation of Lgr3 suppresses the delay in pupariation induced by imaginal disc growth perturbation or ectopic Dilp8 expression.
Title: Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing.

Leucine-rich repeat-containing G protein-coupled receptor 3 (Lgr3) mediates the checkpoint function of Dilp8 for entry into maturation. Two Lgr3-positive neurons in each brain lobe are required to induce a developmental delay upon overexpression of Dilp8.
Title: Drosophila Lgr3 Couples Organ Growth with Maturation and Ensures Developmental Stability.

JAK/STAT coordinates cell proliferation with Dilp8-mediated developmental delay in Drosophila disc regeneration
Title: During Drosophila disc regeneration, JAK/STAT coordinates cell proliferation with Dilp8-mediated developmental delay.

The following Ilp8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ilp8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18785	NM_140692.3 Latest version!	Drosophila melanogaster Insulin-like peptide 8 (Ilp8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ilp8 ( NM_140692.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648949.2 Drosophila melanogaster Insulin-like peptide 8 (Ilp8), mRNA.

Price & Availability↑

CloneID	OFa18785
Clone ID Related Accession (Same CDS sequence)	NM_140692.3
Accession Version	NM_140692.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Insulin-like peptide 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_140692.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTCAA AGTTGCATAT GTGCCGCTGG ATGCTGCTGG TCATCGGAGT CTGTTGCCTG 
ATGGGCAGCT CGTCGGGCAG CTTCTGCTCC CTGGAGCGGA TGAAGAAGTT CGCGATGGAG 
GCGTGCGAGC ACCTCTTTCA GGCGGACGAG GGTGCCCGCC GCGACAGAAG GTCCATCGAG 
TTCGCGCACC ACCATCTGAA TCGACTTGGA TCCGGCAAAA CGCACAACAA GCATCACTAC 
ATCAGCCGAA GCAGCTATCC AATGGGTGGC TACCTGAAGG TGACCCGGGA GCACTTCAAT 
CGCCTCAGCG AACTGGACAT CTTTCCGCGC TACAAGCCCA TCAAGCCGCA CCACGAAAAG 
AAGCATCGCT TCAAGAGGGA TCACTCGAGC AGGTCCTACA ACAACATTCC CTACTGCTGT 
CTAAACCAGT GCGAGGAGGA GTTCTTCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648949.2
CDS	184..636
Translation	MSSKLHMCRWMLLVIGVCCLMGSSSGSFCSLERMKKFAMEACEHLFQADEGARRDRRSIEFAHHHLNRLGSGKTHNKHHYISRSSYPMGGYLKVTREHFNRLSELDIFPRYKPIKPHHEKKHRFKRDHSSRSYNNIPYCCLNQCEEEFFC

Target ORF information:

RefSeq Version	NM_140692.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Insulin-like peptide 8 (Ilp8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140692.3

ORF Insert Sequence:

ATGAGTTCAA AGTTGCATAT GTGCCGCTGG ATGCTGCTGG TCATCGGAGT CTGTTGCCTG 
ATGGGCAGCT CGTCGGGCAG CTTCTGCTCC CTGGAGCGGA TGAAGAAGTT CGCGATGGAG 
GCGTGCGAGC ACCTCTTTCA GGCGGACGAG GGTGCCCGCC GCGACAGAAG GTCCATCGAG 
TTCGCGCACC ACCATCTGAA TCGACTTGGA TCCGGCAAAA CGCACAACAA GCATCACTAC 
ATCAGCCGAA GCAGCTATCC AATGGGTGGC TACCTGAAGG TGACCCGGGA GCACTTCAAT 
CGCCTCAGCG AACTGGACAT CTTTCCGCGC TACAAGCCCA TCAAGCCGCA CCACGAAAAG 
AAGCATCGCT TCAAGAGGGA TCACTCGAGC AGGTCCTACA ACAACATTCC CTACTGCTGT 
CTAAACCAGT GCGAGGAGGA GTTCTTCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ilp8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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