dpn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dpn
Entrez Gene ID	35800
Full Name	deadpan
Synonyms	44C,CG8704,DPN,Dmel\CG8704,Dpn,anon-EST:fe1B12,bHLHe50
General protein information	Preferred Names deadpan Names CG8704 gene product from transcript CG8704-RA CG8704-PA anon-fast-evolving-1B12 dpn-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44C2-44C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057575.3	NP_476923.1	deadpan

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

N activity is required to promote dpn transcription; only in R7 photoreceptor precursors does the removal of Ttk coincide with high N activity; and only in this cell does Dpn expression result
Title: R7 Photoreceptor Specification in the Developing Drosophila Eye: The Role of the Transcription Factor Deadpan.

Dpn contributes to the establishment of Cut expression.
Title: Deadpan contributes to the robustness of the notch response.

E(spl) and Dpn act together to maintain the neuroblasts in a self-renewing state, a process in which they are assisted by Notch, which sustains expression of the E(spl) subset
Title: bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and mediate Notch-induced overproliferation.

Data suggest that Dpn plays important roles in maintaining NB self-renewal and specification of type II NBs in larval brains and that Dpn and Notch function independently in regulating type II NB proliferation and specification.
Title: The bHLH repressor Deadpan regulates the self-renewal and specification of Drosophila larval neural stem cells independently of Notch.

In addition to dpn, Notch signaling must be regulating other genes during this process that act redundantly with dpn.
Title: The bHLH factor deadpan is a direct target of Notch signaling and regulates neuroblast self-renewal in Drosophila.

The following dpn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dpn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09270	NM_057575.3 Latest version!	Drosophila melanogaster deadpan (dpn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dpn ( NM_057575.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476923.1 Drosophila melanogaster deadpan (dpn), mRNA.

Price & Availability↑

CloneID	OFa09270
Clone ID Related Accession (Same CDS sequence)	NM_057575.3
Accession Version	NM_057575.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deadpan
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057575.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTACA AAAACGATAT TAATTCCGAC GACGATTTCG ACTGCTCCAA CGGCTATAGC 
GACAGCTATG GCTCCAATGG ACGCATGTCC AATCCCAATG GATTGTCAAA AGCAGAACTT 
AGAAAGACCA ACAAACCGAT TATGGAGAAA CGTCGCCGAG CTCGCATTAA TCACTGCCTC 
AACGAACTGA AGTCCCTTAT CCTGGAGGCG ATGAAAAAAG ACCCGGCTCG TCATACCAAA 
CTGGAGAAGG CCGACATACT TGAGATGACG GTGAAGCATC TGCAGTCGGT GCAGCGCCAG 
CAGCTCAACA TGGCCATCCA ATCGGATCCC AGCGTTGTCC AGAAGTTCAA GACGGGATTC 
GTTGAGTGCG CGGAGGAGGT GAACCGCTAT GTAAGCCAAA TGGATGGCAT CGATACCGGT 
GTTCGTCAGC GGCTCAGCGC CCATCTTAAC CAGTGTGCCA ATAGTCTGGA GCAGATCGGT 
TCGATGAGCA ACTTCAGCAA TGGATACCGT GGTGGCTTGT TTCCCGCCAC AGCTGTCACG 
GCTGCTCCCA CTCCGCTGTT TCCCTCGTTG CCGCAGGACT TGAACAACAA CAGCCGCACG 
GAGTCATCGG CTCCGGCCAT CCAAATGGGT GGACTTCAGC TAATTCCCTC CCGCCTGCCC 
TCGGGAGAGT TTGCCCTCAT CATGCCGAAC ACAGGTTCCG CTGCTCCGCC TCCGGGACCC 
TTTGCCTGGC CAGGATCTGC AGCGGGAGTC GCAGCTGGAA CAGCCAGTGC CGCCTTGGCC 
AGCATTGCCA ATCCCACACA TCTAAATGAC TATACACAGA GCTTCAGGAT GAGCGCGTTC 
AGCAAACCGG TCAATACCTC AGTTCCGGCC AATCTTCCGG AAAACCTCAT CCACACACTG 
CCCGGCCAAA CGCAGCTGCC GGTGAAGAAC AGCACAAGCC CACCGCTGAG CCCAATCTCC 
TCCATCTCCA GCCACTGCGA GGAGTCCCGG GCTGCCTCGC CCACCGTGGA TGTGATGAGC 
AAGCACAGTT TCGCCGGCGT CTTCTCAACT CCGCCACCAA CCAGCGCGGA GACCTCCTTC 
AACACCAGCG GATCCCTCAA CTTGAGTGCC GGAAGCCACG ACAGCAGCGG ATGCTCGCGA 
CCGCTGGCCC ACTTGCAGCA ACAGCAAGTG AGCTCCACCA GCGGAATCGC CAAGCGCGAC 
AGGGAGGCGG AGGCCGAGTC CAGCGACTGC TCCCTGGACG AGCCCTCGTC CAAGAAATTC 
CTAGCTGGCG CCATCGAAAA GTCGAGCTCC GCTTGGAGGC CGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476923.1
CDS	283..1590
Translation	MDYKNDINSDDDFDCSNGYSDSYGSNGRMSNPNGLSKAELRKTNKPIMEKRRRARINHCLNELKSLILEAMKKDPARHTKLEKADILEMTVKHLQSVQRQQLNMAIQSDPSVVQKFKTGFVECAEEVNRYVSQMDGIDTGVRQRLSAHLNQCANSLEQIGSMSNFSNGYRGGLFPATAVTAAPTPLFPSLPQDLNNNSRTESSAPAIQMGGLQLIPSRLPSGEFALIMPNTGSAAPPPGPFAWPGSAAGVAAGTASAALASIANPTHLNDYTQSFRMSAFSKPVNTSVPANLPENLIHTLPGQTQLPVKNSTSPPLSPISSISSHCEESRAASPTVDVMSKHSFAGVFSTPPPTSAETSFNTSGSLNLSAGSHDSSGCSRPLAHLQQQQVSSTSGIAKRDREAEAESSDCSLDEPSSKKFLAGAIEKSSSAWRPW

Target ORF information:

RefSeq Version	NM_057575.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deadpan (dpn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057575.3

ORF Insert Sequence:

ATGGATTACA AAAACGATAT TAATTCCGAC GACGATTTCG ACTGCTCCAA CGGCTATAGC 
GACAGCTATG GCTCCAATGG ACGCATGTCC AATCCCAATG GATTGTCAAA AGCAGAACTT 
AGAAAGACCA ACAAACCGAT TATGGAGAAA CGTCGCCGAG CTCGCATTAA TCACTGCCTC 
AACGAACTGA AGTCCCTTAT CCTGGAGGCG ATGAAAAAAG ACCCGGCTCG TCATACCAAA 
CTGGAGAAGG CCGACATACT TGAGATGACG GTGAAGCATC TGCAGTCGGT GCAGCGCCAG 
CAGCTCAACA TGGCCATCCA ATCGGATCCC AGCGTTGTCC AGAAGTTCAA GACGGGATTC 
GTTGAGTGCG CGGAGGAGGT GAACCGCTAT GTAAGCCAAA TGGATGGCAT CGATACCGGT 
GTTCGTCAGC GGCTCAGCGC CCATCTTAAC CAGTGTGCCA ATAGTCTGGA GCAGATCGGT 
TCGATGAGCA ACTTCAGCAA TGGATACCGT GGTGGCTTGT TTCCCGCCAC AGCTGTCACG 
GCTGCTCCCA CTCCGCTGTT TCCCTCGTTG CCGCAGGACT TGAACAACAA CAGCCGCACG 
GAGTCATCGG CTCCGGCCAT CCAAATGGGT GGACTTCAGC TAATTCCCTC CCGCCTGCCC 
TCGGGAGAGT TTGCCCTCAT CATGCCGAAC ACAGGTTCCG CTGCTCCGCC TCCGGGACCC 
TTTGCCTGGC CAGGATCTGC AGCGGGAGTC GCAGCTGGAA CAGCCAGTGC CGCCTTGGCC 
AGCATTGCCA ATCCCACACA TCTAAATGAC TATACACAGA GCTTCAGGAT GAGCGCGTTC 
AGCAAACCGG TCAATACCTC AGTTCCGGCC AATCTTCCGG AAAACCTCAT CCACACACTG 
CCCGGCCAAA CGCAGCTGCC GGTGAAGAAC AGCACAAGCC CACCGCTGAG CCCAATCTCC 
TCCATCTCCA GCCACTGCGA GGAGTCCCGG GCTGCCTCGC CCACCGTGGA TGTGATGAGC 
AAGCACAGTT TCGCCGGCGT CTTCTCAACT CCGCCACCAA CCAGCGCGGA GACCTCCTTC 
AACACCAGCG GATCCCTCAA CTTGAGTGCC GGAAGCCACG ACAGCAGCGG ATGCTCGCGA 
CCGCTGGCCC ACTTGCAGCA ACAGCAAGTG AGCTCCACCA GCGGAATCGC CAAGCGCGAC 
AGGGAGGCGG AGGCCGAGTC CAGCGACTGC TCCCTGGACG AGCCCTCGTC CAAGAAATTC 
CTAGCTGGCG CCATCGAAAA GTCGAGCTCC GCTTGGAGGC CGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dpn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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