NFAT cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFAT
Entrez Gene ID	32321
Full Name	NFAT nuclear factor
Synonyms	CG11172,Dmel\CG11172,EP1335,MESR1,NF-AT5,NFAT5,Nfat,dNFAT
General protein information	Preferred Names NFAT nuclear factor Names CG11172 gene product from transcript CG11172-RA CG11172-PA CG11172-PB CG11172-PC CG11172-PD CG11172-PE CG11172-PG CG11172-PH NFAT homolog NFAT-PA NFAT-PB NFAT-PC NFAT-PD NFAT-PE NFAT-PG NFAT-PH Rel domain protein drosophila TonEBP misexpression suppressor of Ras 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12A9-12B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169276.3	NP_001162747.1	NFAT nuclear factor, isoform E
NM_001272597.2	NP_001259526.2	NFAT nuclear factor, isoform G
NM_001169274.2	NP_001162745.1	NFAT nuclear factor, isoform C
NM_001169275.2	NP_001162746.1	NFAT nuclear factor, isoform D
NM_001103504.2	NP_001096974.1	NFAT nuclear factor, isoform B
NM_001298290.1	NP_001285219.1	NFAT nuclear factor, isoform H
NM_078592.3	NP_511147.2	NFAT nuclear factor, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nur77 is a novel negative-feedback regulator of pulmonary artery smooth muscle cell proliferation through inhibition of the signal transducer and activator of transcription 3/Pim-1/nuclear factor of activated T cells axis
Title: Nur77 suppresses pulmonary artery smooth muscle cell proliferation through inhibition of the STAT3/Pim-1/NFAT pathway.

We propose that NFAT regulates pre-synaptic development and constrains long-term plasticity by dampening neuronal excitability
Title: NFAT regulates pre-synaptic development and activity-dependent plasticity in Drosophila.

The following NFAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01524	NM_078592.3 Latest version!	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa01525	NM_001103504.2 Latest version!	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa03538	NM_001169276.3 Latest version!	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa03554	NM_001169275.2 Latest version!	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa03558	NM_001169274.2 Latest version!	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa03558	NM_001298290.1 Latest version!	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa21461	NM_001272597.2 Latest version!	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFAT ( NM_078592.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511147.2 Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01524
Clone ID Related Accession (Same CDS sequence)	NM_078592.3
Accession Version	NM_078592.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NFAT nuclear factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078592.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTTTGA AATTCACAAA AACCAGTGGC GCTGGACCCT CGGCCGTACC GCTACTGACC 
ACCGCCCCCG CGACAGTCAC CAGTTCGGCG TCCATGCAGG CAACAACAAC AAGTTACGCG 
GCCCGTGGAA CAGGCATGAG AATGACCATG TCCACCAATT CCACAATGAG TGCGCGTATA 
CATCGAAAAG GATTTCGCAT ACCATCGAAA AGACAGCCGG GTAAGGGATT GCCCGGCAAG 
TTGCACACCA TAACCAGGAC GGGTCCTGGC AAAATGGTTC CTGGGAAGCG AATACCACAG 
CGACCCCATC CGCCGCCCTG CGACAACTCG AACGACAGTG GGCTGGGTTT CGATCAGCAC 
ACGGAGCTGA GATCCTCGGC AGGAGCTGGG GGCGTTACTG ATCCGGCGGC CAATGGAGGC 
AGTGGCTCCA ACTCTGGCCA GCGATCCAGT TTGCTGGTCA ATAGCGCTTT AACCAACACA 
GTGGCTGCTG CAGCAGCCGC AGCAGCTGCC GCGGTGGCCA GCAACACGTT GCAGCAGCAT 
CAGCAGCACC ATCAACAGCA GCAGCAGCAA CAGCAGCAAC AACAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAGCAGCA GCAACAACAT TCACCGCAGC AGCATTTAAT CAGAGCGATA 
CCTGTATCAA GATCGCGGAA CAACACGAAA GTGATGGCTC ACTGTCTGGA GGATCTGGAC 
CTAATATCGG CGTCCTCGGA GGACAGCTCG ACCATCGCAA CGCCGGCCGG TTTGGACGAG 
GACACGGGCT TTGTGAACGA CACCAAAACG ACGACAACCG GTGGCAATAG TAGTTCGGAT 
CCATTTGGAG GCGTCTTTCA GGCAACCGAG GCAGCCGTGG CAGCAGCCGT TAATGCCTTC 
AACTTGCAAC AGCAGCAGCA TTCGGTGCAA CAGCAACAGC ATCAACTCTT TCAGTACAAC 
GGATTGCTGT TGCAGCCGCA GCAGCAATTG CAGCAGCAAC AGTCGCATCA CCAACAACAA 
CAACAACAGC AACAGCATCA CCGCCATGGC AAGCATATCA AACGCAAGAA GCTCGAGTGC 
AACCAAGTGG AACTGGACAA CGATGATGCC TGCAGCGAGG ATGAGTTCAT TCGCAAGATT 
GCCAGTTCGG TAACGATGTC ACCCACGCTT GAGGCCACGC CCTCGCCGCC GCCCATTGTC 
GCCTCCGTTG CACCTGCCAC GACCATTGTT AGCCTTCTGC CGCTCATCAG CAGCGGCAAC 
AGCAACAACA ACAACAACAA CAACAACATT GAGACAAAAT TCATATCGGC CAGGAATGTC 
ACGCGTGTGG CGAACAAACG TCAACCCACT ACACCGCTGA ACAGCGTCGC CAGCTCCAAC 
GATGGCCAGG TGCAGCTGGA GATTTTGTCG CAGCCGGAGC AACAGCACCG GGCACGCTAT 
CAAACGGAGG GCAGCCGCGG TGCCGTCAAG GATCGCAGCG GCAATGGATT CCCGATCGTC 
CGACTGACCG GATACGATAA GGTTGCCGTC CTCCAGGTCT TCATCGGCAC GGACATTGGA 
CGCGTGGCAC CGCACATGTT CTATCAGGCC TGCAAGGTTG CCGGCAAGAA CTCGACGCAG 
TGCAACGAGA AGAAGGTCGA CGGCACCATG GTCATTGAGA TCGATTTCAA GCCCGAAACG 
GACATGACCA TCACATGCGA TTGCGTTGGA ATCTTGAAGG AACGCAATGT GGATGTGGAG 
CACCGATTTC CCGAGCATCT GGCGCAAAAG AACAAGAAGA AATCGACTCG CTGCCGGATG 
GTGTTCCGCA CCCAGCTGAC CCGTGACGAT GGCACCACCG AGACCCTCCA GGTCTGCTCG 
AATCCAATCA TCTGCACTCA ACCACCTGGC GTGCCGGAAA TATGCAAGAA ATCCTTAAAC 
TCATGTCCCG TTGATGGCGG CCTCGAGCTT TTCATTATCG GCAAGAACTT TCTGAAGGAC 
ACACACGTGG TGTTTCAGGA GACATACGAC AGCGTCAACG GTGACGATCC GGCCACGGAA 
ATTGCAGTGC GCCAGCAGCT CATCGGCGGT ACAGCCGCTC TTTGGGAACA GAGTGTTCTG 
CCGGATAAGG AGTACCTTCA CCAGACTCAT CTGATATGCA CGGTGCCACC GTATCTGCAC 
CAGAACATCC TGAAACCGGT CCAGGTTCAA GTGTCGATCG TCTCCAGCGG CAAGAAGAGC 
GAACCGCATA CGTTCACCTA CACGGCCAAG GGACAGTACA CGACGCTGGC GGCGGCCAGC 
ACGTTAAGTA ACACAATCCA CGCCCAAGAT GTGAGCGGTT TCATGGACAC CACATCAGCG 
TCCAATGCGA GTGGCTCCAG TGGTTGGAGT GCGGCAGGCG GTTCCGGCAA CAATACATCT 
ACAGGCGATA ATGTGGAGGC CAAACACGAG ATCGATTCGG GCATGATGCC TCCGCCCATT 
ACCACCCAAA TACCGATGGG CGTTCGACGC TCCTCGCTGC CCAGTGTCAC ACCCATGATC 
ACGGACCAAC AGCTGGTCCA TCTCAACGCA GTGGCGGCCA GTGCCGAGGC CCTGAAGACG 
GAACTCGACG ACAGCAACTC GCATAGCCCA CTGACCGGTG AATCCACACC GGATAGCCCC 
AACGCAGCCT TGCAGTATCA TCGCTTTGCA CGAAAGCCCA GCCTGGACAC CATTATGTAC 
GATCAGTCGA ACAGTCTGCC CGGTTTCCCG ACCGTTGTGG CGCCGGCTAC AGCTGCCGCC 
GTGGCCGCTG CAGTTGATAT TGATCCGACG GCGGTGGCCG TGGCGGTGGA ACTGGCCGTA 
AAAAATGAGA TTGTCAAGCA TGTCGTACAG CAGCACCAGC AGATGCAGGA GCAAATGCAC 
GAGCAGCAGC AGCAGCAACA GCAGCAGCAA CAACAACAAC AACAGCAGCA ACAACAACAA 
CCCCAACAGC AGCAGCAGCA ACAGGTGCAC AAGTTTATTG ACGAGCTGAC CAAATCCACA 
TCGGTGGTTT CCAGCAATGG TACCACCGAA CCAGCTCTGT TCACCACCTC GGCGGTGATC 
GATCATGCCC TGACCGATAT TCTGCAGGCC AAAGTTGGCA TTGCGCCGCC CAATGTGGTG 
CTGGAAAGGA GTTTGTCCCT AAGCTCCACA AATTCCAGCA GCTCCATGAG CGGCAGTGAG 
AGTTCGCCGA ATAGTTCACC TTTGACGCAG GATATCATAC TCAATTCAGA ACCGGCAGCA 
GCTCTGGCCG GAGCTGCTGC CTTGGGTGGC CCGGCGCCTG TCGATGTCAC CGGTGGTCTG 
TCCACCGATA TCATTATGAA TCCCGCCGTT TCACCATCGA CTATTTTGTG CTCCGCAAAT 
GGAGCGGCCA CTGCCGTAGT GCCGAATATA CTCGCACCTC ACCAAGTGAC CATGGCCAAC 
TCCATTTTGA ACGATATTGC CATGCAGCCG GAACCAACAC AGCAGGATGC AGCAGTAGCA 
GCCTTGGCGT TGAGCAACAT CATGATGAGT CCACCCACCG CGGCATCGGG TGTGGGAGTT 
GGTGACACAC TGCCACCAAC ACCAGCTACT ATGCAGCCAG AGGTCGCAGC CACTGCCACA 
TCCACTGCGG TCAGCAACAT GATTATTAAG GCGGCGGCGG ACTTTATAAC CACCCAGGAG 
CAGGAGCAAC ACCACTACCA TCACCACGGT CGCATCCACT CCCATTCGCC ACAGGCAGCT 
GTCGGAGTGA GCGGTAATCC TGCCGAGACG GCATCGGATC CATTGGTTGA CCTGCTATTG 
AACCACTCAA ACACACCAGA AACAGCGGCT GCTGCCGCTG CCGCAGTAGC TGTAGAAGCA 
GCCAACTTCC AGTCGATGAA TCACAGTCAC CACAGCCATC ATGGTCATCA TGGTCACAGT 
CACGGCCACG GCCATAGTCG CGTCACAGGA CACGTATCCG GGCATGTGAC CAGCCACCAT 
CGTGGTCACC ACTTGCCGGT GGTGCCGCCG ACACCACAAG AATCTCTGAT CGTTGCGCTG 
GCCAGCGAGA ATGCTCTGCA AAAGTCGGTG GCCACCGCCG CAGTGACCAC CAATGGAGCA 
GTGATGACGC AACAGGCATC TGCCCCGAAC ACAGCGGGTA GCATACTTCC GGCAGCGGTT 
GGAGCGGTAG CTGCGGCGGC CGCCGTGGCG GTGCAGCCGC CCATTCCCCA GGAGCTGACC 
ACGATGTCGG ATCAGGATCT GATCAGCTAC ATCAACCCGA GCACCTTTGA TCAGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511147.2
CDS	713..4972
Translation	MLLKFTKTSGAGPSAVPLLTTAPATVTSSASMQATTTSYAARGTGMRMTMSTNSTMSARIHRKGFRIPSKRQPGKGLPGKLHTITRTGPGKMVPGKRIPQRPHPPPCDNSNDSGLGFDQHTELRSSAGAGGVTDPAANGGSGSNSGQRSSLLVNSALTNTVAAAAAAAAAAVASNTLQQHQQHHQQQQQQQQQQQQQQQQQQQQQQQQQHSPQQHLIRAIPVSRSRNNTKVMAHCLEDLDLISASSEDSSTIATPAGLDEDTGFVNDTKTTTTGGNSSSDPFGGVFQATEAAVAAAVNAFNLQQQQHSVQQQQHQLFQYNGLLLQPQQQLQQQQSHHQQQQQQQQHHRHGKHIKRKKLECNQVELDNDDACSEDEFIRKIASSVTMSPTLEATPSPPPIVASVAPATTIVSLLPLISSGNSNNNNNNNNIETKFISARNVTRVANKRQPTTPLNSVASSNDGQVQLEILSQPEQQHRARYQTEGSRGAVKDRSGNGFPIVRLTGYDKVAVLQVFIGTDIGRVAPHMFYQACKVAGKNSTQCNEKKVDGTMVIEIDFKPETDMTITCDCVGILKERNVDVEHRFPEHLAQKNKKKSTRCRMVFRTQLTRDDGTTETLQVCSNPIICTQPPGVPEICKKSLNSCPVDGGLELFIIGKNFLKDTHVVFQETYDSVNGDDPATEIAVRQQLIGGTAALWEQSVLPDKEYLHQTHLICTVPPYLHQNILKPVQVQVSIVSSGKKSEPHTFTYTAKGQYTTLAAASTLSNTIHAQDVSGFMDTTSASNASGSSGWSAAGGSGNNTSTGDNVEAKHEIDSGMMPPPITTQIPMGVRRSSLPSVTPMITDQQLVHLNAVAASAEALKTELDDSNSHSPLTGESTPDSPNAALQYHRFARKPSLDTIMYDQSNSLPGFPTVVAPATAAAVAAAVDIDPTAVAVAVELAVKNEIVKHVVQQHQQMQEQMHEQQQQQQQQQQQQQQQQQQQPQQQQQQQVHKFIDELTKSTSVVSSNGTTEPALFTTSAVIDHALTDILQAKVGIAPPNVVLERSLSLSSTNSSSSMSGSESSPNSSPLTQDIILNSEPAAALAGAAALGGPAPVDVTGGLSTDIIMNPAVSPSTILCSANGAATAVVPNILAPHQVTMANSILNDIAMQPEPTQQDAAVAALALSNIMMSPPTAASGVGVGDTLPPTPATMQPEVAATATSTAVSNMIIKAAADFITTQEQEQHHYHHHGRIHSHSPQAAVGVSGNPAETASDPLVDLLLNHSNTPETAAAAAAAVAVEAANFQSMNHSHHSHHGHHGHSHGHGHSRVTGHVSGHVTSHHRGHHLPVVPPTPQESLIVALASENALQKSVATAAVTTNGAVMTQQASAPNTAGSILPAAVGAVAAAAAVAVQPPIPQELTTMSDQDLISYINPSTFDQL

Target ORF information:

RefSeq Version	NM_078592.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078592.3

ORF Insert Sequence:

ATGCTTTTGA AATTCACAAA AACCAGTGGC GCTGGACCCT CGGCCGTACC GCTACTGACC 
ACCGCCCCCG CGACAGTCAC CAGTTCGGCG TCCATGCAGG CAACAACAAC AAGTTACGCG 
GCCCGTGGAA CAGGCATGAG AATGACCATG TCCACCAATT CCACAATGAG TGCGCGTATA 
CATCGAAAAG GATTTCGCAT ACCATCGAAA AGACAGCCGG GTAAGGGATT GCCCGGCAAG 
TTGCACACCA TAACCAGGAC GGGTCCTGGC AAAATGGTTC CTGGGAAGCG AATACCACAG 
CGACCCCATC CGCCGCCCTG CGACAACTCG AACGACAGTG GGCTGGGTTT CGATCAGCAC 
ACGGAGCTGA GATCCTCGGC AGGAGCTGGG GGCGTTACTG ATCCGGCGGC CAATGGAGGC 
AGTGGCTCCA ACTCTGGCCA GCGATCCAGT TTGCTGGTCA ATAGCGCTTT AACCAACACA 
GTGGCTGCTG CAGCAGCCGC AGCAGCTGCC GCGGTGGCCA GCAACACGTT GCAGCAGCAT 
CAGCAGCACC ATCAACAGCA GCAGCAGCAA CAGCAGCAAC AACAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAGCAGCA GCAACAACAT TCACCGCAGC AGCATTTAAT CAGAGCGATA 
CCTGTATCAA GATCGCGGAA CAACACGAAA GTGATGGCTC ACTGTCTGGA GGATCTGGAC 
CTAATATCGG CGTCCTCGGA GGACAGCTCG ACCATCGCAA CGCCGGCCGG TTTGGACGAG 
GACACGGGCT TTGTGAACGA CACCAAAACG ACGACAACCG GTGGCAATAG TAGTTCGGAT 
CCATTTGGAG GCGTCTTTCA GGCAACCGAG GCAGCCGTGG CAGCAGCCGT TAATGCCTTC 
AACTTGCAAC AGCAGCAGCA TTCGGTGCAA CAGCAACAGC ATCAACTCTT TCAGTACAAC 
GGATTGCTGT TGCAGCCGCA GCAGCAATTG CAGCAGCAAC AGTCGCATCA CCAACAACAA 
CAACAACAGC AACAGCATCA CCGCCATGGC AAGCATATCA AACGCAAGAA GCTCGAGTGC 
AACCAAGTGG AACTGGACAA CGATGATGCC TGCAGCGAGG ATGAGTTCAT TCGCAAGATT 
GCCAGTTCGG TAACGATGTC ACCCACGCTT GAGGCCACGC CCTCGCCGCC GCCCATTGTC 
GCCTCCGTTG CACCTGCCAC GACCATTGTT AGCCTTCTGC CGCTCATCAG CAGCGGCAAC 
AGCAACAACA ACAACAACAA CAACAACATT GAGACAAAAT TCATATCGGC CAGGAATGTC 
ACGCGTGTGG CGAACAAACG TCAACCCACT ACACCGCTGA ACAGCGTCGC CAGCTCCAAC 
GATGGCCAGG TGCAGCTGGA GATTTTGTCG CAGCCGGAGC AACAGCACCG GGCACGCTAT 
CAAACGGAGG GCAGCCGCGG TGCCGTCAAG GATCGCAGCG GCAATGGATT CCCGATCGTC 
CGACTGACCG GATACGATAA GGTTGCCGTC CTCCAGGTCT TCATCGGCAC GGACATTGGA 
CGCGTGGCAC CGCACATGTT CTATCAGGCC TGCAAGGTTG CCGGCAAGAA CTCGACGCAG 
TGCAACGAGA AGAAGGTCGA CGGCACCATG GTCATTGAGA TCGATTTCAA GCCCGAAACG 
GACATGACCA TCACATGCGA TTGCGTTGGA ATCTTGAAGG AACGCAATGT GGATGTGGAG 
CACCGATTTC CCGAGCATCT GGCGCAAAAG AACAAGAAGA AATCGACTCG CTGCCGGATG 
GTGTTCCGCA CCCAGCTGAC CCGTGACGAT GGCACCACCG AGACCCTCCA GGTCTGCTCG 
AATCCAATCA TCTGCACTCA ACCACCTGGC GTGCCGGAAA TATGCAAGAA ATCCTTAAAC 
TCATGTCCCG TTGATGGCGG CCTCGAGCTT TTCATTATCG GCAAGAACTT TCTGAAGGAC 
ACACACGTGG TGTTTCAGGA GACATACGAC AGCGTCAACG GTGACGATCC GGCCACGGAA 
ATTGCAGTGC GCCAGCAGCT CATCGGCGGT ACAGCCGCTC TTTGGGAACA GAGTGTTCTG 
CCGGATAAGG AGTACCTTCA CCAGACTCAT CTGATATGCA CGGTGCCACC GTATCTGCAC 
CAGAACATCC TGAAACCGGT CCAGGTTCAA GTGTCGATCG TCTCCAGCGG CAAGAAGAGC 
GAACCGCATA CGTTCACCTA CACGGCCAAG GGACAGTACA CGACGCTGGC GGCGGCCAGC 
ACGTTAAGTA ACACAATCCA CGCCCAAGAT GTGAGCGGTT TCATGGACAC CACATCAGCG 
TCCAATGCGA GTGGCTCCAG TGGTTGGAGT GCGGCAGGCG GTTCCGGCAA CAATACATCT 
ACAGGCGATA ATGTGGAGGC CAAACACGAG ATCGATTCGG GCATGATGCC TCCGCCCATT 
ACCACCCAAA TACCGATGGG CGTTCGACGC TCCTCGCTGC CCAGTGTCAC ACCCATGATC 
ACGGACCAAC AGCTGGTCCA TCTCAACGCA GTGGCGGCCA GTGCCGAGGC CCTGAAGACG 
GAACTCGACG ACAGCAACTC GCATAGCCCA CTGACCGGTG AATCCACACC GGATAGCCCC 
AACGCAGCCT TGCAGTATCA TCGCTTTGCA CGAAAGCCCA GCCTGGACAC CATTATGTAC 
GATCAGTCGA ACAGTCTGCC CGGTTTCCCG ACCGTTGTGG CGCCGGCTAC AGCTGCCGCC 
GTGGCCGCTG CAGTTGATAT TGATCCGACG GCGGTGGCCG TGGCGGTGGA ACTGGCCGTA 
AAAAATGAGA TTGTCAAGCA TGTCGTACAG CAGCACCAGC AGATGCAGGA GCAAATGCAC 
GAGCAGCAGC AGCAGCAACA GCAGCAGCAA CAACAACAAC AACAGCAGCA ACAACAACAA 
CCCCAACAGC AGCAGCAGCA ACAGGTGCAC AAGTTTATTG ACGAGCTGAC CAAATCCACA 
TCGGTGGTTT CCAGCAATGG TACCACCGAA CCAGCTCTGT TCACCACCTC GGCGGTGATC 
GATCATGCCC TGACCGATAT TCTGCAGGCC AAAGTTGGCA TTGCGCCGCC CAATGTGGTG 
CTGGAAAGGA GTTTGTCCCT AAGCTCCACA AATTCCAGCA GCTCCATGAG CGGCAGTGAG 
AGTTCGCCGA ATAGTTCACC TTTGACGCAG GATATCATAC TCAATTCAGA ACCGGCAGCA 
GCTCTGGCCG GAGCTGCTGC CTTGGGTGGC CCGGCGCCTG TCGATGTCAC CGGTGGTCTG 
TCCACCGATA TCATTATGAA TCCCGCCGTT TCACCATCGA CTATTTTGTG CTCCGCAAAT 
GGAGCGGCCA CTGCCGTAGT GCCGAATATA CTCGCACCTC ACCAAGTGAC CATGGCCAAC 
TCCATTTTGA ACGATATTGC CATGCAGCCG GAACCAACAC AGCAGGATGC AGCAGTAGCA 
GCCTTGGCGT TGAGCAACAT CATGATGAGT CCACCCACCG CGGCATCGGG TGTGGGAGTT 
GGTGACACAC TGCCACCAAC ACCAGCTACT ATGCAGCCAG AGGTCGCAGC CACTGCCACA 
TCCACTGCGG TCAGCAACAT GATTATTAAG GCGGCGGCGG ACTTTATAAC CACCCAGGAG 
CAGGAGCAAC ACCACTACCA TCACCACGGT CGCATCCACT CCCATTCGCC ACAGGCAGCT 
GTCGGAGTGA GCGGTAATCC TGCCGAGACG GCATCGGATC CATTGGTTGA CCTGCTATTG 
AACCACTCAA ACACACCAGA AACAGCGGCT GCTGCCGCTG CCGCAGTAGC TGTAGAAGCA 
GCCAACTTCC AGTCGATGAA TCACAGTCAC CACAGCCATC ATGGTCATCA TGGTCACAGT 
CACGGCCACG GCCATAGTCG CGTCACAGGA CACGTATCCG GGCATGTGAC CAGCCACCAT 
CGTGGTCACC ACTTGCCGGT GGTGCCGCCG ACACCACAAG AATCTCTGAT CGTTGCGCTG 
GCCAGCGAGA ATGCTCTGCA AAAGTCGGTG GCCACCGCCG CAGTGACCAC CAATGGAGCA 
GTGATGACGC AACAGGCATC TGCCCCGAAC ACAGCGGGTA GCATACTTCC GGCAGCGGTT 
GGAGCGGTAG CTGCGGCGGC CGCCGTGGCG GTGCAGCCGC CCATTCCCCA GGAGCTGACC 
ACGATGTCGG ATCAGGATCT GATCAGCTAC ATCAACCCGA GCACCTTTGA TCAGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT ( NM_001103504.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096974.1 Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01525
Clone ID Related Accession (Same CDS sequence)	NM_001103504.2
Accession Version	NM_001103504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NFAT nuclear factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001103504.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTCA CCTACAATCA GTATAAACAT TATGAGTCCG GTTATCGGAT TCCATCTAAA 
ATGCATAATC TGAGCCATAA TAACAACAAT AATAGTAATA ATAATAATAA TGGGGGTGGC 
CACACCAACA ACAACAACAA TGGCAACAAT AGTGGCCATC ACTATGCCCA TCACAATAAT 
AGCAACAATG GCGGCAATAA CAACACAAAT TTCCAAGCCT ATCAGCATCA TTACAAAAGT 
AATTTCGGCA TGAGAATGAC CATGTCCACC AATTCCACAA TGAGTGCGCG TATACATCGA 
AAAGGATTTC GCATACCATC GAAAAGACAG CCGGGTAAGG GATTGCCCGG CAAGTTGCAC 
ACCATAACCA GGACGGGTCC TGGCAAAATG GTTCCTGGGA AGCGAATACC ACAGCGACCC 
CATCCGCCGC CCTGCGACAA CTCGAACGAC AGTGGGCTGG GTTTCGATCA GCACACGGAG 
CTGAGATCCT CGGCAGGAGC TGGGGGCGTT ACTGATCCGG CGGCCAATGG AGGCAGTGGC 
TCCAACTCTG GCCAGCGATC CAGTTTGCTG GTCAATAGCG CTTTAACCAA CACAGTGGCT 
GCTGCAGCAG CCGCAGCAGC TGCCGCGGTG GCCAGCAACA CGTTGCAGCA GCATCAGCAG 
CACCATCAAC AGCAGCAGCA GCAACAGCAG CAACAACAGC AGCAGCAGCA GCAACAACAA 
CAACAGCAGC AGCAGCAACA ACATTCACCG CAGCAGCATT TAATCAGAGC GATACCTGTA 
TCAAGATCGC GGAACAACAC GAAAGTGATG GCTCACTGTC TGGAGGATCT GGACCTAATA 
TCGGCGTCCT CGGAGGACAG CTCGACCATC GCAACGCCGG CCGGTTTGGA CGAGGACACG 
GGCTTTGTGA ACGACACCAA AACGACGACA ACCGGTGGCA ATAGTAGTTC GGATCCATTT 
GGAGGCGTCT TTCAGGCAAC CGAGGCAGCC GTGGCAGCAG CCGTTAATGC CTTCAACTTG 
CAACAGCAGC AGCATTCGGT GCAACAGCAA CAGCATCAAC TCTTTCAGTA CAACGGATTG 
CTGTTGCAGC CGCAGCAGCA ATTGCAGCAG CAACAGTCGC ATCACCAACA ACAACAACAA 
CAGCAACAGC ATCACCGCCA TGGCAAGCAT ATCAAACGCA AGAAGCTCGA GTGCAACCAA 
GTGGAACTGG ACAACGATGA TGCCTGCAGC GAGGATGAGT TCATTCGCAA GATTGCCAGT 
TCGGTAACGA TGTCACCCAC GCTTGAGGCC ACGCCCTCGC CGCCGCCCAT TGTCGCCTCC 
GTTGCACCTG CCACGACCAT TGTTAGCCTT CTGCCGCTCA TCAGCAGCGG CAACAGCAAC 
AACAACAACA ACAACAACAA CATTGAGACA AAATTCATAT CGGCCAGGAA TGTCACGCGT 
GTGGCGAACA AACGTCAACC CACTACACCG CTGAACAGCG TCGCCAGCTC CAACGATGGC 
CAGGTGCAGC TGGAGATTTT GTCGCAGCCG GAGCAACAGC ACCGGGCACG CTATCAAACG 
GAGGGCAGCC GCGGTGCCGT CAAGGATCGC AGCGGCAATG GATTCCCGAT CGTCCGACTG 
ACCGGATACG ATAAGGTTGC CGTCCTCCAG GTCTTCATCG GCACGGACAT TGGACGCGTG 
GCACCGCACA TGTTCTATCA GGCCTGCAAG GTTGCCGGCA AGAACTCGAC GCAGTGCAAC 
GAGAAGAAGG TCGACGGCAC CATGGTCATT GAGATCGATT TCAAGCCCGA AACGGACATG 
ACCATCACAT GCGATTGCGT TGGAATCTTG AAGGAACGCA ATGTGGATGT GGAGCACCGA 
TTTCCCGAGC ATCTGGCGCA AAAGAACAAG AAGAAATCGA CTCGCTGCCG GATGGTGTTC 
CGCACCCAGC TGACCCGTGA CGATGGCACC ACCGAGACCC TCCAGGTCTG CTCGAATCCA 
ATCATCTGCA CTCAACCACC TGGCGTGCCG GAAATATGCA AGAAATCCTT AAACTCATGT 
CCCGTTGATG GCGGCCTCGA GCTTTTCATT ATCGGCAAGA ACTTTCTGAA GGACACACAC 
GTGGTGTTTC AGGAGACATA CGACAGCGTC AACGGTGACG ATCCGGCCAC GGAAATTGCA 
GTGCGCCAGC AGCTCATCGG CGGTACAGCC GCTCTTTGGG AACAGAGTGT TCTGCCGGAT 
AAGGAGTACC TTCACCAGAC TCATCTGATA TGCACGGTGC CACCGTATCT GCACCAGAAC 
ATCCTGAAAC CGGTCCAGGT TCAAGTGTCG ATCGTCTCCA GCGGCAAGAA GAGCGAACCG 
CATACGTTCA CCTACACGGC CAAGGGACAG TACACGACGC TGGCGGCGGC CAGCACGTTA 
AGTAACACAA TCCACGCCCA AGATGTGAGC GGTTTCATGG ACACCACATC AGCGTCCAAT 
GCGAGTGGCT CCAGTGGTTG GAGTGCGGCA GGCGGTTCCG GCAACAATAC ATCTACAGGC 
GATAATGTGG AGGCCAAACA CGAGATCGAT TCGGGCATGA TGCCTCCGCC CATTACCACC 
CAAATACCGA TGGGCGTTCG ACGCTCCTCG CTGCCCAGTG TCACACCCAT GATCACGGAC 
CAACAGCTGG TCCATCTCAA CGCAGTGGCG GCCAGTGCCG AGGCCCTGAA GACGGAACTC 
GACGACAGCA ACTCGCATAG CCCACTGACC GGTGAATCCA CACCGGATAG CCCCAACGCA 
GCCTTGCAGT ATCATCGCTT TGCACGAAAG CCCAGCCTGG ACACCATTAT GTACGATCAG 
TCGAACAGTC TGCCCGGTTT CCCGACCGTT GTGGCGCCGG CTACAGCTGC CGCCGTGGCC 
GCTGCAGTTG ATATTGATCC GACGGCGGTG GCCGTGGCGG TGGAACTGGC CGTAAAAAAT 
GAGATTGTCA AGCATGTCGT ACAGCAGCAC CAGCAGATGC AGGAGCAAAT GCACGAGCAG 
CAGCAGCAGC AACAGCAGCA GCAACAACAA CAACAACAGC AGCAACAACA ACAACCCCAA 
CAGCAGCAGC AGCAACAGGT GCACAAGTTT ATTGACGAGC TGACCAAATC CACATCGGTG 
GTTTCCAGCA ATGGTACCAC CGAACCAGCT CTGTTCACCA CCTCGGCGGT GATCGATCAT 
GCCCTGACCG ATATTCTGCA GGCCAAAGTT GGCATTGCGC CGCCCAATGT GGTGCTGGAA 
AGGAGTTTGT CCCTAAGCTC CACAAATTCC AGCAGCTCCA TGAGCGGCAG TGAGAGTTCG 
CCGAATAGTT CACCTTTGAC GCAGGATATC ATACTCAATT CAGAACCGGC AGCAGCTCTG 
GCCGGAGCTG CTGCCTTGGG TGGCCCGGCG CCTGTCGATG TCACCGGTGG TCTGTCCACC 
GATATCATTA TGAATCCCGC CGTTTCACCA TCGACTATTT TGTGCTCCGC AAATGGAGCG 
GCCACTGCCG TAGTGCCGAA TATACTCGCA CCTCACCAAG TGACCATGGC CAACTCCATT 
TTGAACGATA TTGCCATGCA GCCGGAACCA ACACAGCAGG ATGCAGCAGT AGCAGCCTTG 
GCGTTGAGCA ACATCATGAT GAGTCCACCC ACCGCGGCAT CGGGTGTGGG AGTTGGTGAC 
ACACTGCCAC CAACACCAGC TACTATGCAG CCAGAGGTCG CAGCCACTGC CACATCCACT 
GCGGTCAGCA ACATGATTAT TAAGGCGGCG GCGGACTTTA TAACCACCCA GGAGCAGGAG 
CAACACCACT ACCATCACCA CGGTCGCATC CACTCCCATT CGCCACAGGC AGCTGTCGGA 
GTGAGCGGTA ATCCTGCCGA GACGGCATCG GATCCATTGG TTGACCTGCT ATTGAACCAC 
TCAAACACAC CAGAAACAGC GGCTGCTGCC GCTGCCGCAG TAGCTGTAGA AGCAGCCAAC 
TTCCAGTCGA TGAATCACAG TCACCACAGC CATCATGGTC ATCATGGTCA CAGTCACGGC 
CACGGCCATA GTCGCGTCAC AGGACACGTA TCCGGGCATG TGACCAGCCA CCATCGTGGT 
CACCACTTGC CGGTGGTGCC GCCGACACCA CAAGAATCTC TGATCGTTGC GCTGGCCAGC 
GAGAATGCTC TGCAAAAGTC GGTGGCCACC GCCGCAGTGA CCACCAATGG AGCAGTGATG 
ACGCAACAGG CATCTGCCCC GAACACAGCG GGTAGCATAC TTCCGGCAGC GGTTGGAGCG 
GTAGCTGCGG CGGCCGCCGT GGCGGTGCAG CCGCCCATTC CCCAGGAGCT GACCACGATG 
TCGGATCAGG ATCTGATCAG CTACATCAAC CCGAGCACCT TTGATCAGCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096974.1
CDS	380..4753
Translation	MRFTYNQYKHYESGYRIPSKMHNLSHNNNNNSNNNNNGGGHTNNNNNGNNSGHHYAHHNNSNNGGNNNTNFQAYQHHYKSNFGMRMTMSTNSTMSARIHRKGFRIPSKRQPGKGLPGKLHTITRTGPGKMVPGKRIPQRPHPPPCDNSNDSGLGFDQHTELRSSAGAGGVTDPAANGGSGSNSGQRSSLLVNSALTNTVAAAAAAAAAAVASNTLQQHQQHHQQQQQQQQQQQQQQQQQQQQQQQQQHSPQQHLIRAIPVSRSRNNTKVMAHCLEDLDLISASSEDSSTIATPAGLDEDTGFVNDTKTTTTGGNSSSDPFGGVFQATEAAVAAAVNAFNLQQQQHSVQQQQHQLFQYNGLLLQPQQQLQQQQSHHQQQQQQQQHHRHGKHIKRKKLECNQVELDNDDACSEDEFIRKIASSVTMSPTLEATPSPPPIVASVAPATTIVSLLPLISSGNSNNNNNNNNIETKFISARNVTRVANKRQPTTPLNSVASSNDGQVQLEILSQPEQQHRARYQTEGSRGAVKDRSGNGFPIVRLTGYDKVAVLQVFIGTDIGRVAPHMFYQACKVAGKNSTQCNEKKVDGTMVIEIDFKPETDMTITCDCVGILKERNVDVEHRFPEHLAQKNKKKSTRCRMVFRTQLTRDDGTTETLQVCSNPIICTQPPGVPEICKKSLNSCPVDGGLELFIIGKNFLKDTHVVFQETYDSVNGDDPATEIAVRQQLIGGTAALWEQSVLPDKEYLHQTHLICTVPPYLHQNILKPVQVQVSIVSSGKKSEPHTFTYTAKGQYTTLAAASTLSNTIHAQDVSGFMDTTSASNASGSSGWSAAGGSGNNTSTGDNVEAKHEIDSGMMPPPITTQIPMGVRRSSLPSVTPMITDQQLVHLNAVAASAEALKTELDDSNSHSPLTGESTPDSPNAALQYHRFARKPSLDTIMYDQSNSLPGFPTVVAPATAAAVAAAVDIDPTAVAVAVELAVKNEIVKHVVQQHQQMQEQMHEQQQQQQQQQQQQQQQQQQQPQQQQQQQVHKFIDELTKSTSVVSSNGTTEPALFTTSAVIDHALTDILQAKVGIAPPNVVLERSLSLSSTNSSSSMSGSESSPNSSPLTQDIILNSEPAAALAGAAALGGPAPVDVTGGLSTDIIMNPAVSPSTILCSANGAATAVVPNILAPHQVTMANSILNDIAMQPEPTQQDAAVAALALSNIMMSPPTAASGVGVGDTLPPTPATMQPEVAATATSTAVSNMIIKAAADFITTQEQEQHHYHHHGRIHSHSPQAAVGVSGNPAETASDPLVDLLLNHSNTPETAAAAAAAVAVEAANFQSMNHSHHSHHGHHGHSHGHGHSRVTGHVSGHVTSHHRGHHLPVVPPTPQESLIVALASENALQKSVATAAVTTNGAVMTQQASAPNTAGSILPAAVGAVAAAAAVAVQPPIPQELTTMSDQDLISYINPSTFDQL

Target ORF information:

RefSeq Version	NM_001103504.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103504.2

ORF Insert Sequence:

ATGCGATTCA CCTACAATCA GTATAAACAT TATGAGTCCG GTTATCGGAT TCCATCTAAA 
ATGCATAATC TGAGCCATAA TAACAACAAT AATAGTAATA ATAATAATAA TGGGGGTGGC 
CACACCAACA ACAACAACAA TGGCAACAAT AGTGGCCATC ACTATGCCCA TCACAATAAT 
AGCAACAATG GCGGCAATAA CAACACAAAT TTCCAAGCCT ATCAGCATCA TTACAAAAGT 
AATTTCGGCA TGAGAATGAC CATGTCCACC AATTCCACAA TGAGTGCGCG TATACATCGA 
AAAGGATTTC GCATACCATC GAAAAGACAG CCGGGTAAGG GATTGCCCGG CAAGTTGCAC 
ACCATAACCA GGACGGGTCC TGGCAAAATG GTTCCTGGGA AGCGAATACC ACAGCGACCC 
CATCCGCCGC CCTGCGACAA CTCGAACGAC AGTGGGCTGG GTTTCGATCA GCACACGGAG 
CTGAGATCCT CGGCAGGAGC TGGGGGCGTT ACTGATCCGG CGGCCAATGG AGGCAGTGGC 
TCCAACTCTG GCCAGCGATC CAGTTTGCTG GTCAATAGCG CTTTAACCAA CACAGTGGCT 
GCTGCAGCAG CCGCAGCAGC TGCCGCGGTG GCCAGCAACA CGTTGCAGCA GCATCAGCAG 
CACCATCAAC AGCAGCAGCA GCAACAGCAG CAACAACAGC AGCAGCAGCA GCAACAACAA 
CAACAGCAGC AGCAGCAACA ACATTCACCG CAGCAGCATT TAATCAGAGC GATACCTGTA 
TCAAGATCGC GGAACAACAC GAAAGTGATG GCTCACTGTC TGGAGGATCT GGACCTAATA 
TCGGCGTCCT CGGAGGACAG CTCGACCATC GCAACGCCGG CCGGTTTGGA CGAGGACACG 
GGCTTTGTGA ACGACACCAA AACGACGACA ACCGGTGGCA ATAGTAGTTC GGATCCATTT 
GGAGGCGTCT TTCAGGCAAC CGAGGCAGCC GTGGCAGCAG CCGTTAATGC CTTCAACTTG 
CAACAGCAGC AGCATTCGGT GCAACAGCAA CAGCATCAAC TCTTTCAGTA CAACGGATTG 
CTGTTGCAGC CGCAGCAGCA ATTGCAGCAG CAACAGTCGC ATCACCAACA ACAACAACAA 
CAGCAACAGC ATCACCGCCA TGGCAAGCAT ATCAAACGCA AGAAGCTCGA GTGCAACCAA 
GTGGAACTGG ACAACGATGA TGCCTGCAGC GAGGATGAGT TCATTCGCAA GATTGCCAGT 
TCGGTAACGA TGTCACCCAC GCTTGAGGCC ACGCCCTCGC CGCCGCCCAT TGTCGCCTCC 
GTTGCACCTG CCACGACCAT TGTTAGCCTT CTGCCGCTCA TCAGCAGCGG CAACAGCAAC 
AACAACAACA ACAACAACAA CATTGAGACA AAATTCATAT CGGCCAGGAA TGTCACGCGT 
GTGGCGAACA AACGTCAACC CACTACACCG CTGAACAGCG TCGCCAGCTC CAACGATGGC 
CAGGTGCAGC TGGAGATTTT GTCGCAGCCG GAGCAACAGC ACCGGGCACG CTATCAAACG 
GAGGGCAGCC GCGGTGCCGT CAAGGATCGC AGCGGCAATG GATTCCCGAT CGTCCGACTG 
ACCGGATACG ATAAGGTTGC CGTCCTCCAG GTCTTCATCG GCACGGACAT TGGACGCGTG 
GCACCGCACA TGTTCTATCA GGCCTGCAAG GTTGCCGGCA AGAACTCGAC GCAGTGCAAC 
GAGAAGAAGG TCGACGGCAC CATGGTCATT GAGATCGATT TCAAGCCCGA AACGGACATG 
ACCATCACAT GCGATTGCGT TGGAATCTTG AAGGAACGCA ATGTGGATGT GGAGCACCGA 
TTTCCCGAGC ATCTGGCGCA AAAGAACAAG AAGAAATCGA CTCGCTGCCG GATGGTGTTC 
CGCACCCAGC TGACCCGTGA CGATGGCACC ACCGAGACCC TCCAGGTCTG CTCGAATCCA 
ATCATCTGCA CTCAACCACC TGGCGTGCCG GAAATATGCA AGAAATCCTT AAACTCATGT 
CCCGTTGATG GCGGCCTCGA GCTTTTCATT ATCGGCAAGA ACTTTCTGAA GGACACACAC 
GTGGTGTTTC AGGAGACATA CGACAGCGTC AACGGTGACG ATCCGGCCAC GGAAATTGCA 
GTGCGCCAGC AGCTCATCGG CGGTACAGCC GCTCTTTGGG AACAGAGTGT TCTGCCGGAT 
AAGGAGTACC TTCACCAGAC TCATCTGATA TGCACGGTGC CACCGTATCT GCACCAGAAC 
ATCCTGAAAC CGGTCCAGGT TCAAGTGTCG ATCGTCTCCA GCGGCAAGAA GAGCGAACCG 
CATACGTTCA CCTACACGGC CAAGGGACAG TACACGACGC TGGCGGCGGC CAGCACGTTA 
AGTAACACAA TCCACGCCCA AGATGTGAGC GGTTTCATGG ACACCACATC AGCGTCCAAT 
GCGAGTGGCT CCAGTGGTTG GAGTGCGGCA GGCGGTTCCG GCAACAATAC ATCTACAGGC 
GATAATGTGG AGGCCAAACA CGAGATCGAT TCGGGCATGA TGCCTCCGCC CATTACCACC 
CAAATACCGA TGGGCGTTCG ACGCTCCTCG CTGCCCAGTG TCACACCCAT GATCACGGAC 
CAACAGCTGG TCCATCTCAA CGCAGTGGCG GCCAGTGCCG AGGCCCTGAA GACGGAACTC 
GACGACAGCA ACTCGCATAG CCCACTGACC GGTGAATCCA CACCGGATAG CCCCAACGCA 
GCCTTGCAGT ATCATCGCTT TGCACGAAAG CCCAGCCTGG ACACCATTAT GTACGATCAG 
TCGAACAGTC TGCCCGGTTT CCCGACCGTT GTGGCGCCGG CTACAGCTGC CGCCGTGGCC 
GCTGCAGTTG ATATTGATCC GACGGCGGTG GCCGTGGCGG TGGAACTGGC CGTAAAAAAT 
GAGATTGTCA AGCATGTCGT ACAGCAGCAC CAGCAGATGC AGGAGCAAAT GCACGAGCAG 
CAGCAGCAGC AACAGCAGCA GCAACAACAA CAACAACAGC AGCAACAACA ACAACCCCAA 
CAGCAGCAGC AGCAACAGGT GCACAAGTTT ATTGACGAGC TGACCAAATC CACATCGGTG 
GTTTCCAGCA ATGGTACCAC CGAACCAGCT CTGTTCACCA CCTCGGCGGT GATCGATCAT 
GCCCTGACCG ATATTCTGCA GGCCAAAGTT GGCATTGCGC CGCCCAATGT GGTGCTGGAA 
AGGAGTTTGT CCCTAAGCTC CACAAATTCC AGCAGCTCCA TGAGCGGCAG TGAGAGTTCG 
CCGAATAGTT CACCTTTGAC GCAGGATATC ATACTCAATT CAGAACCGGC AGCAGCTCTG 
GCCGGAGCTG CTGCCTTGGG TGGCCCGGCG CCTGTCGATG TCACCGGTGG TCTGTCCACC 
GATATCATTA TGAATCCCGC CGTTTCACCA TCGACTATTT TGTGCTCCGC AAATGGAGCG 
GCCACTGCCG TAGTGCCGAA TATACTCGCA CCTCACCAAG TGACCATGGC CAACTCCATT 
TTGAACGATA TTGCCATGCA GCCGGAACCA ACACAGCAGG ATGCAGCAGT AGCAGCCTTG 
GCGTTGAGCA ACATCATGAT GAGTCCACCC ACCGCGGCAT CGGGTGTGGG AGTTGGTGAC 
ACACTGCCAC CAACACCAGC TACTATGCAG CCAGAGGTCG CAGCCACTGC CACATCCACT 
GCGGTCAGCA ACATGATTAT TAAGGCGGCG GCGGACTTTA TAACCACCCA GGAGCAGGAG 
CAACACCACT ACCATCACCA CGGTCGCATC CACTCCCATT CGCCACAGGC AGCTGTCGGA 
GTGAGCGGTA ATCCTGCCGA GACGGCATCG GATCCATTGG TTGACCTGCT ATTGAACCAC 
TCAAACACAC CAGAAACAGC GGCTGCTGCC GCTGCCGCAG TAGCTGTAGA AGCAGCCAAC 
TTCCAGTCGA TGAATCACAG TCACCACAGC CATCATGGTC ATCATGGTCA CAGTCACGGC 
CACGGCCATA GTCGCGTCAC AGGACACGTA TCCGGGCATG TGACCAGCCA CCATCGTGGT 
CACCACTTGC CGGTGGTGCC GCCGACACCA CAAGAATCTC TGATCGTTGC GCTGGCCAGC 
GAGAATGCTC TGCAAAAGTC GGTGGCCACC GCCGCAGTGA CCACCAATGG AGCAGTGATG 
ACGCAACAGG CATCTGCCCC GAACACAGCG GGTAGCATAC TTCCGGCAGC GGTTGGAGCG 
GTAGCTGCGG CGGCCGCCGT GGCGGTGCAG CCGCCCATTC CCCAGGAGCT GACCACGATG 
TCGGATCAGG ATCTGATCAG CTACATCAAC CCGAGCACCT TTGATCAGCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT ( NM_001169276.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162747.1 Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant E, mRNA.

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CloneID	OFa03538
Clone ID Related Accession (Same CDS sequence)	NM_001169276.3
Accession Version	NM_001169276.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NFAT nuclear factor, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169276.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTCA CCTACAATCA GTATAAACAT TATGAGTCCG GTTATCGGAT TCCATCTAAA 
ATGCATAATC TGAGCCATAA TAACAACAAT AATAGTAATA ATAATAATAA TGGGGGTGGC 
CACACCAACA ACAACAACAA TGGCAACAAT AGTGGCCATC ACTATGCCCA TCACAATAAT 
AGCAACAATG GCGGCAATAA CAACACAAAT TTCCAAGCCT ATCAGCATCA TTACAAAAGT 
AATTTCGGCA TGAGAATGAC CATGTCCACC AATTCCACAA TGAGTGCGCG TATACATCGA 
AAAGGATTTC GCATACCATC GAAAAGACAG CCGGGTAAGG GATTGCCCGG CAAGTTGCAC 
ACCATAACCA GGACGGGTCC TGGCAAAATG GTTCCTGGGA AGCGAATACC ACAGCGACCC 
CATCCGCCGC CCTGCGACAA CTCGAACGAC AGTGGGCTGG GTTTCGATCA GCACACGGAG 
CTGAGATCCT CGGCAGGAGC TGGGGGCGTT ACTGATCCGG CGGCCAATGG AGGCAGTGGC 
TCCAACTCTG GCCAGCGATC CAGTTTGCTG GTCAATAGCG CTTTAACCAA CACAGTGGCT 
GCTGCAGCAG CCGCAGCAGC TGCCGCGGTG GCCAGCAACA CGTTGCAGCA GCATCAGCAG 
CACCATCAAC AGCAGCAGCA GCAACAGCAG CAACAACAGC AGCAGCAGCA GCAACAACAA 
CAACAGCAGC AGCAGCAACA ACATTCACCG CAGCAGCATT TAATCAGAGC GATACCTGTA 
TCAAGATTCA TATCGGCCAG GAATGTCACG CGTGTGGCGA ACAAACGTCA ACCCACTACA 
CCGCTGAACA GCGTCGCCAG CTCCAACGAT GGCCAGGTGC AGCTGGAGAT TTTGTCGCAG 
CCGGAGCAAC AGCACCGGGC ACGCTATCAA ACGGAGGGCA GCCGCGGTGC CGTCAAGGAT 
CGCAGCGGCA ATGGATTCCC GATCGTCCGA CTGACCGGAT ACGATAAGGT TGCCGTCCTC 
CAGGTCTTCA TCGGCACGGA CATTGGACGC GTGGCACCGC ACATGTTCTA TCAGGCCTGC 
AAGGTTGCCG GCAAGAACTC GACGCAGTGC AACGAGAAGA AGGTCGACGG CACCATGGTC 
ATTGAGATCG ATTTCAAGCC CGAAACGGAC ATGACCATCA CATGCGATTG CGTTGGAATC 
TTGAAGGAAC GCAATGTGGA TGTGGAGCAC CGATTTCCCG AGCATCTGGC GCAAAAGAAC 
AAGAAGAAAT CGACTCGCTG CCGGATGGTG TTCCGCACCC AGCTGACCCG TGACGATGGC 
ACCACCGAGA CCCTCCAGGT CTGCTCGAAT CCAATCATCT GCACTCAACC ACCTGGCGTG 
CCGGAAATAT GCAAGAAATC CTTAAACTCA TGTCCCGTTG ATGGCGGCCT CGAGCTTTTC 
ATTATCGGCA AGAACTTTCT GAAGGACACA CACGTGGTGT TTCAGGAGAC ATACGACAGC 
GTCAACGGTG ACGATCCGGC CACGGAAATT GCAGTGCGCC AGCAGCTCAT CGGCGGTACA 
GCCGCTCTTT GGGAACAGAG TGTTCTGCCG GATAAGGAGT ACCTTCACCA GACTCATCTG 
ATATGCACGG TGCCACCGTA TCTGCACCAG AACATCCTGA AACCGGTCCA GGTTCAAGTG 
TCGATCGTCT CCAGCGGCAA GAAGAGCGAA CCGCATACGT TCACCTACAC GGCCAAGGGA 
CAGTACACGA CGCTGGCGGC GGCCAGCACG TTAAGTAACA CAATCCACGC CCAAGCAATT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162747.1
CDS	380..2182
Translation	MRFTYNQYKHYESGYRIPSKMHNLSHNNNNNSNNNNNGGGHTNNNNNGNNSGHHYAHHNNSNNGGNNNTNFQAYQHHYKSNFGMRMTMSTNSTMSARIHRKGFRIPSKRQPGKGLPGKLHTITRTGPGKMVPGKRIPQRPHPPPCDNSNDSGLGFDQHTELRSSAGAGGVTDPAANGGSGSNSGQRSSLLVNSALTNTVAAAAAAAAAAVASNTLQQHQQHHQQQQQQQQQQQQQQQQQQQQQQQQQHSPQQHLIRAIPVSRFISARNVTRVANKRQPTTPLNSVASSNDGQVQLEILSQPEQQHRARYQTEGSRGAVKDRSGNGFPIVRLTGYDKVAVLQVFIGTDIGRVAPHMFYQACKVAGKNSTQCNEKKVDGTMVIEIDFKPETDMTITCDCVGILKERNVDVEHRFPEHLAQKNKKKSTRCRMVFRTQLTRDDGTTETLQVCSNPIICTQPPGVPEICKKSLNSCPVDGGLELFIIGKNFLKDTHVVFQETYDSVNGDDPATEIAVRQQLIGGTAALWEQSVLPDKEYLHQTHLICTVPPYLHQNILKPVQVQVSIVSSGKKSEPHTFTYTAKGQYTTLAAASTLSNTIHAQAI

Target ORF information:

RefSeq Version	NM_001169276.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169276.3

ORF Insert Sequence:

ATGCGATTCA CCTACAATCA GTATAAACAT TATGAGTCCG GTTATCGGAT TCCATCTAAA 
ATGCATAATC TGAGCCATAA TAACAACAAT AATAGTAATA ATAATAATAA TGGGGGTGGC 
CACACCAACA ACAACAACAA TGGCAACAAT AGTGGCCATC ACTATGCCCA TCACAATAAT 
AGCAACAATG GCGGCAATAA CAACACAAAT TTCCAAGCCT ATCAGCATCA TTACAAAAGT 
AATTTCGGCA TGAGAATGAC CATGTCCACC AATTCCACAA TGAGTGCGCG TATACATCGA 
AAAGGATTTC GCATACCATC GAAAAGACAG CCGGGTAAGG GATTGCCCGG CAAGTTGCAC 
ACCATAACCA GGACGGGTCC TGGCAAAATG GTTCCTGGGA AGCGAATACC ACAGCGACCC 
CATCCGCCGC CCTGCGACAA CTCGAACGAC AGTGGGCTGG GTTTCGATCA GCACACGGAG 
CTGAGATCCT CGGCAGGAGC TGGGGGCGTT ACTGATCCGG CGGCCAATGG AGGCAGTGGC 
TCCAACTCTG GCCAGCGATC CAGTTTGCTG GTCAATAGCG CTTTAACCAA CACAGTGGCT 
GCTGCAGCAG CCGCAGCAGC TGCCGCGGTG GCCAGCAACA CGTTGCAGCA GCATCAGCAG 
CACCATCAAC AGCAGCAGCA GCAACAGCAG CAACAACAGC AGCAGCAGCA GCAACAACAA 
CAACAGCAGC AGCAGCAACA ACATTCACCG CAGCAGCATT TAATCAGAGC GATACCTGTA 
TCAAGATTCA TATCGGCCAG GAATGTCACG CGTGTGGCGA ACAAACGTCA ACCCACTACA 
CCGCTGAACA GCGTCGCCAG CTCCAACGAT GGCCAGGTGC AGCTGGAGAT TTTGTCGCAG 
CCGGAGCAAC AGCACCGGGC ACGCTATCAA ACGGAGGGCA GCCGCGGTGC CGTCAAGGAT 
CGCAGCGGCA ATGGATTCCC GATCGTCCGA CTGACCGGAT ACGATAAGGT TGCCGTCCTC 
CAGGTCTTCA TCGGCACGGA CATTGGACGC GTGGCACCGC ACATGTTCTA TCAGGCCTGC 
AAGGTTGCCG GCAAGAACTC GACGCAGTGC AACGAGAAGA AGGTCGACGG CACCATGGTC 
ATTGAGATCG ATTTCAAGCC CGAAACGGAC ATGACCATCA CATGCGATTG CGTTGGAATC 
TTGAAGGAAC GCAATGTGGA TGTGGAGCAC CGATTTCCCG AGCATCTGGC GCAAAAGAAC 
AAGAAGAAAT CGACTCGCTG CCGGATGGTG TTCCGCACCC AGCTGACCCG TGACGATGGC 
ACCACCGAGA CCCTCCAGGT CTGCTCGAAT CCAATCATCT GCACTCAACC ACCTGGCGTG 
CCGGAAATAT GCAAGAAATC CTTAAACTCA TGTCCCGTTG ATGGCGGCCT CGAGCTTTTC 
ATTATCGGCA AGAACTTTCT GAAGGACACA CACGTGGTGT TTCAGGAGAC ATACGACAGC 
GTCAACGGTG ACGATCCGGC CACGGAAATT GCAGTGCGCC AGCAGCTCAT CGGCGGTACA 
GCCGCTCTTT GGGAACAGAG TGTTCTGCCG GATAAGGAGT ACCTTCACCA GACTCATCTG 
ATATGCACGG TGCCACCGTA TCTGCACCAG AACATCCTGA AACCGGTCCA GGTTCAAGTG 
TCGATCGTCT CCAGCGGCAA GAAGAGCGAA CCGCATACGT TCACCTACAC GGCCAAGGGA 
CAGTACACGA CGCTGGCGGC GGCCAGCACG TTAAGTAACA CAATCCACGC CCAAGCAATT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT ( NM_001169275.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162746.1 Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant D, mRNA.

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CloneID	OFa03554
Clone ID Related Accession (Same CDS sequence)	NM_001169275.2
Accession Version	NM_001169275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NFAT nuclear factor, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169275.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTTTGA AATTCACAAA AACCAGTGGC GCTGGACCCT CGGCCGTACC GCTACTGACC 
ACCGCCCCCG CGACAGTCAC CAGTTCGGCG TCCATGCAGG CAACAACAAC AAGTTACGCG 
GCCCGTGGAA CAGGCATGAG AATGACCATG TCCACCAATT CCACAATGAG TGCGCGTATA 
CATCGAAAAG GATTTCGCAT ACCATCGAAA AGACAGCCGG GTAAGGGATT GCCCGGCAAG 
TTGCACACCA TAACCAGGAC GGGTCCTGGC AAAATGGTTC CTGGGAAGCG AATACCACAG 
CGACCCCATC CGCCGCCCTG CGACAACTCG AACGACAGTG GGCTGGGTTT CGATCAGCAC 
ACGGAGCTGA GATCCTCGGC AGGAGCTGGG GGCGTTACTG ATCCGGCGGC CAATGGAGGC 
AGTGGCTCCA ACTCTGGCCA GCGATCCAGT TTGCTGGTCA ATAGCGCTTT AACCAACACA 
GTGGCTGCTG CAGCAGCCGC AGCAGCTGCC GCGGTGGCCA GCAACACGTT GCAGCAGCAT 
CAGCAGCACC ATCAACAGCA GCAGCAGCAA CAGCAGCAAC AACAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAGCAGCA GCAACAACAT TCACCGCAGC AGCATTTAAT CAGAGCGATA 
CCTGTATCAA GATTCATATC GGCCAGGAAT GTCACGCGTG TGGCGAACAA ACGTCAACCC 
ACTACACCGC TGAACAGCGT CGCCAGCTCC AACGATGGCC AGGTGCAGCT GGAGATTTTG 
TCGCAGCCGG AGCAACAGCA CCGGGCACGC TATCAAACGG AGGGCAGCCG CGGTGCCGTC 
AAGGATCGCA GCGGCAATGG ATTCCCGATC GTCCGACTGA CCGGATACGA TAAGGTTGCC 
GTCCTCCAGG TCTTCATCGG CACGGACATT GGACGCGTGG CACCGCACAT GTTCTATCAG 
GCCTGCAAGG TTGCCGGCAA GAACTCGACG CAGTGCAACG AGAAGAAGGT CGACGGCACC 
ATGGTCATTG AGATCGATTT CAAGCCCGAA ACGGACATGA CCATCACATG CGATTGCGTT 
GGAATCTTGA AGGAACGCAA TGTGGATGTG GAGCACCGAT TTCCCGAGCA TCTGGCGCAA 
AAGAACAAGA AGAAATCGAC TCGCTGCCGG ATGGTGTTCC GCACCCAGCT GACCCGTGAC 
GATGGCACCA CCGAGACCCT CCAGGTCTGC TCGAATCCAA TCATCTGCAC TCAACCACCT 
GGCGTGCCGG AAATATGCAA GAAATCCTTA AACTCATGTC CCGTTGATGG CGGCCTCGAG 
CTTTTCATTA TCGGCAAGAA CTTTCTGAAG GACACACACG TGGTGTTTCA GGAGACATAC 
GACAGCGTCA ACGGTGACGA TCCGGCCACG GAAATTGCAG TGCGCCAGCA GCTCATCGGC 
GGTACAGCCG CTCTTTGGGA ACAGAGTGTT CTGCCGGATA AGGAGTACCT TCACCAGACT 
CATCTGATAT GCACGGTGCC ACCGTATCTG CACCAGAACA TCCTGAAACC GGTCCAGGTT 
CAAGTGTCGA TCGTCTCCAG CGGCAAGAAG AGCGAACCGC ATACGTTCAC CTACACGGCC 
AAGGGACAGT ACACGACGCT GGCGGCGGCC AGCACGTTAA GTAACACAAT CCACGCCCAA 
GATGTGAGCG GTTTCATGGA CACCACATCA GCGTCCAATG CGAGTGGCTC CAGTGGTTGG 
AGTGCGGCAG GCGGTTCCGG CAACAATACA TCTACAGGCG ATAATGTGGA GGCCAAACAC 
GAGATCGATT CGGGCATGAT GCCTCCGCCC ATTACCACCC AAATACCGAT GGGCGTTCGA 
CGCTCCTCGC TGCCCAGTGT CACACCCATG ATCACGGACC AACAGCTGGT CCATCTCAAC 
GCAGTGGCGG CCAGTGCCGA GGCCCTGAAG ACGGAACTCG ACGACAGCAA CTCGCATAGC 
CCACTGACCG GTGAATCCAC ACCGGATAGC CCCAACGCAG CCTTGCAGTA TCATCGCTTT 
GCACGAAAGC CCAGCCTGGA CACCATTATG TACGATCAGT CGAACAGTCT GCCCGGTTTC 
CCGACCGTTG TGGCGCCGGC TACAGCTGCC GCCGTGGCCG CTGCAGTTGA TATTGATCCG 
ACGGCGGTGG CCGTGGCGGT GGAACTGGCC GTAAAAAATG AGATTGTCAA GCATGTCGTA 
CAGCAGCACC AGCAGATGCA GGAGCAAATG CACGAGCAGC AGCAGCAGCA ACAGCAGCAG 
CAACAACAAC AACAACAGCA GCAACAACAA CAACCCCAAC AGCAGCAGCA GCAACAGGTG 
CACAAGTTTA TTGACGAGCT GACCAAATCC ACATCGGTGG TTTCCAGCAA TGGTACCACC 
GAACCAGCTC TGTTCACCAC CTCGGCGGTG ATCGATCATG CCCTGACCGA TATTCTGCAG 
GCCAAAGTTG GCATTGCGCC GCCCAATGTG GTGCTGGAAA GGAGTTTGTC CCTAAGCTCC 
ACAAATTCCA GCAGCTCCAT GAGCGGCAGT GAGAGTTCGC CGAATAGTTC ACCTTTGACG 
CAGGATATCA TACTCAATTC AGAACCGGCA GCAGCTCTGG CCGGAGCTGC TGCCTTGGGT 
GGCCCGGCGC CTGTCGATGT CACCGGTGGT CTGTCCACCG ATATCATTAT GAATCCCGCC 
GTTTCACCAT CGACTATTTT GTGCTCCGCA AATGGAGCGG CCACTGCCGT AGTGCCGAAT 
ATACTCGCAC CTCACCAAGT GACCATGGCC AACTCCATTT TGAACGATAT TGCCATGCAG 
CCGGAACCAA CACAGCAGGA TGCAGCAGTA GCAGCCTTGG CGTTGAGCAA CATCATGATG 
AGTCCACCCA CCGCGGCATC GGGTGTGGGA GTTGGTGACA CACTGCCACC AACACCAGCT 
ACTATGCAGC CAGAGGTCGC AGCCACTGCC ACATCCACTG CGGTCAGCAA CATGATTATT 
AAGGCGGCGG CGGACTTTAT AACCACCCAG GAGCAGGAGC AACACCACTA CCATCACCAC 
GGTCGCATCC ACTCCCATTC GCCACAGGCA GCTGTCGGAG TGAGCGGTAA TCCTGCCGAG 
ACGGCATCGG ATCCATTGGT TGACCTGCTA TTGAACCACT CAAACACACC AGAAACAGCG 
GCTGCTGCCG CTGCCGCAGT AGCTGTAGAA GCAGCCAACT TCCAGTCGAT GAATCACAGT 
CACCACAGCC ATCATGGTCA TCATGGTCAC AGTCACGGCC ACGGCCATAG TCGCGTCACA 
GGACACGTAT CCGGGCATGT GACCAGCCAC CATCGTGGTC ACCACTTGCC GGTGGTGCCG 
CCGACACCAC AAGAATCTCT GATCGTTGCG CTGGCCAGCG AGAATGCTCT GCAAAAGTCG 
GTGGCCACCG CCGCAGTGAC CACCAATGGA GCAGTGATGA CGCAACAGGC ATCTGCCCCG 
AACACAGCGG GTAGCATACT TCCGGCAGCG GTTGGAGCGG TAGCTGCGGC GGCCGCCGTG 
GCGGTGCAGC CGCCCATTCC CCAGGAGCTG ACCACGATGT CGGATCAGGA TCTGATCAGC 
TACATCAACC CGAGCACCTT TGATCAGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162746.1
CDS	713..4345
Translation	MLLKFTKTSGAGPSAVPLLTTAPATVTSSASMQATTTSYAARGTGMRMTMSTNSTMSARIHRKGFRIPSKRQPGKGLPGKLHTITRTGPGKMVPGKRIPQRPHPPPCDNSNDSGLGFDQHTELRSSAGAGGVTDPAANGGSGSNSGQRSSLLVNSALTNTVAAAAAAAAAAVASNTLQQHQQHHQQQQQQQQQQQQQQQQQQQQQQQQQHSPQQHLIRAIPVSRFISARNVTRVANKRQPTTPLNSVASSNDGQVQLEILSQPEQQHRARYQTEGSRGAVKDRSGNGFPIVRLTGYDKVAVLQVFIGTDIGRVAPHMFYQACKVAGKNSTQCNEKKVDGTMVIEIDFKPETDMTITCDCVGILKERNVDVEHRFPEHLAQKNKKKSTRCRMVFRTQLTRDDGTTETLQVCSNPIICTQPPGVPEICKKSLNSCPVDGGLELFIIGKNFLKDTHVVFQETYDSVNGDDPATEIAVRQQLIGGTAALWEQSVLPDKEYLHQTHLICTVPPYLHQNILKPVQVQVSIVSSGKKSEPHTFTYTAKGQYTTLAAASTLSNTIHAQDVSGFMDTTSASNASGSSGWSAAGGSGNNTSTGDNVEAKHEIDSGMMPPPITTQIPMGVRRSSLPSVTPMITDQQLVHLNAVAASAEALKTELDDSNSHSPLTGESTPDSPNAALQYHRFARKPSLDTIMYDQSNSLPGFPTVVAPATAAAVAAAVDIDPTAVAVAVELAVKNEIVKHVVQQHQQMQEQMHEQQQQQQQQQQQQQQQQQQQPQQQQQQQVHKFIDELTKSTSVVSSNGTTEPALFTTSAVIDHALTDILQAKVGIAPPNVVLERSLSLSSTNSSSSMSGSESSPNSSPLTQDIILNSEPAAALAGAAALGGPAPVDVTGGLSTDIIMNPAVSPSTILCSANGAATAVVPNILAPHQVTMANSILNDIAMQPEPTQQDAAVAALALSNIMMSPPTAASGVGVGDTLPPTPATMQPEVAATATSTAVSNMIIKAAADFITTQEQEQHHYHHHGRIHSHSPQAAVGVSGNPAETASDPLVDLLLNHSNTPETAAAAAAAVAVEAANFQSMNHSHHSHHGHHGHSHGHGHSRVTGHVSGHVTSHHRGHHLPVVPPTPQESLIVALASENALQKSVATAAVTTNGAVMTQQASAPNTAGSILPAAVGAVAAAAAVAVQPPIPQELTTMSDQDLISYINPSTFDQL

Target ORF information:

RefSeq Version	NM_001169275.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169275.2

ORF Insert Sequence:

ATGCTTTTGA AATTCACAAA AACCAGTGGC GCTGGACCCT CGGCCGTACC GCTACTGACC 
ACCGCCCCCG CGACAGTCAC CAGTTCGGCG TCCATGCAGG CAACAACAAC AAGTTACGCG 
GCCCGTGGAA CAGGCATGAG AATGACCATG TCCACCAATT CCACAATGAG TGCGCGTATA 
CATCGAAAAG GATTTCGCAT ACCATCGAAA AGACAGCCGG GTAAGGGATT GCCCGGCAAG 
TTGCACACCA TAACCAGGAC GGGTCCTGGC AAAATGGTTC CTGGGAAGCG AATACCACAG 
CGACCCCATC CGCCGCCCTG CGACAACTCG AACGACAGTG GGCTGGGTTT CGATCAGCAC 
ACGGAGCTGA GATCCTCGGC AGGAGCTGGG GGCGTTACTG ATCCGGCGGC CAATGGAGGC 
AGTGGCTCCA ACTCTGGCCA GCGATCCAGT TTGCTGGTCA ATAGCGCTTT AACCAACACA 
GTGGCTGCTG CAGCAGCCGC AGCAGCTGCC GCGGTGGCCA GCAACACGTT GCAGCAGCAT 
CAGCAGCACC ATCAACAGCA GCAGCAGCAA CAGCAGCAAC AACAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAGCAGCA GCAACAACAT TCACCGCAGC AGCATTTAAT CAGAGCGATA 
CCTGTATCAA GATTCATATC GGCCAGGAAT GTCACGCGTG TGGCGAACAA ACGTCAACCC 
ACTACACCGC TGAACAGCGT CGCCAGCTCC AACGATGGCC AGGTGCAGCT GGAGATTTTG 
TCGCAGCCGG AGCAACAGCA CCGGGCACGC TATCAAACGG AGGGCAGCCG CGGTGCCGTC 
AAGGATCGCA GCGGCAATGG ATTCCCGATC GTCCGACTGA CCGGATACGA TAAGGTTGCC 
GTCCTCCAGG TCTTCATCGG CACGGACATT GGACGCGTGG CACCGCACAT GTTCTATCAG 
GCCTGCAAGG TTGCCGGCAA GAACTCGACG CAGTGCAACG AGAAGAAGGT CGACGGCACC 
ATGGTCATTG AGATCGATTT CAAGCCCGAA ACGGACATGA CCATCACATG CGATTGCGTT 
GGAATCTTGA AGGAACGCAA TGTGGATGTG GAGCACCGAT TTCCCGAGCA TCTGGCGCAA 
AAGAACAAGA AGAAATCGAC TCGCTGCCGG ATGGTGTTCC GCACCCAGCT GACCCGTGAC 
GATGGCACCA CCGAGACCCT CCAGGTCTGC TCGAATCCAA TCATCTGCAC TCAACCACCT 
GGCGTGCCGG AAATATGCAA GAAATCCTTA AACTCATGTC CCGTTGATGG CGGCCTCGAG 
CTTTTCATTA TCGGCAAGAA CTTTCTGAAG GACACACACG TGGTGTTTCA GGAGACATAC 
GACAGCGTCA ACGGTGACGA TCCGGCCACG GAAATTGCAG TGCGCCAGCA GCTCATCGGC 
GGTACAGCCG CTCTTTGGGA ACAGAGTGTT CTGCCGGATA AGGAGTACCT TCACCAGACT 
CATCTGATAT GCACGGTGCC ACCGTATCTG CACCAGAACA TCCTGAAACC GGTCCAGGTT 
CAAGTGTCGA TCGTCTCCAG CGGCAAGAAG AGCGAACCGC ATACGTTCAC CTACACGGCC 
AAGGGACAGT ACACGACGCT GGCGGCGGCC AGCACGTTAA GTAACACAAT CCACGCCCAA 
GATGTGAGCG GTTTCATGGA CACCACATCA GCGTCCAATG CGAGTGGCTC CAGTGGTTGG 
AGTGCGGCAG GCGGTTCCGG CAACAATACA TCTACAGGCG ATAATGTGGA GGCCAAACAC 
GAGATCGATT CGGGCATGAT GCCTCCGCCC ATTACCACCC AAATACCGAT GGGCGTTCGA 
CGCTCCTCGC TGCCCAGTGT CACACCCATG ATCACGGACC AACAGCTGGT CCATCTCAAC 
GCAGTGGCGG CCAGTGCCGA GGCCCTGAAG ACGGAACTCG ACGACAGCAA CTCGCATAGC 
CCACTGACCG GTGAATCCAC ACCGGATAGC CCCAACGCAG CCTTGCAGTA TCATCGCTTT 
GCACGAAAGC CCAGCCTGGA CACCATTATG TACGATCAGT CGAACAGTCT GCCCGGTTTC 
CCGACCGTTG TGGCGCCGGC TACAGCTGCC GCCGTGGCCG CTGCAGTTGA TATTGATCCG 
ACGGCGGTGG CCGTGGCGGT GGAACTGGCC GTAAAAAATG AGATTGTCAA GCATGTCGTA 
CAGCAGCACC AGCAGATGCA GGAGCAAATG CACGAGCAGC AGCAGCAGCA ACAGCAGCAG 
CAACAACAAC AACAACAGCA GCAACAACAA CAACCCCAAC AGCAGCAGCA GCAACAGGTG 
CACAAGTTTA TTGACGAGCT GACCAAATCC ACATCGGTGG TTTCCAGCAA TGGTACCACC 
GAACCAGCTC TGTTCACCAC CTCGGCGGTG ATCGATCATG CCCTGACCGA TATTCTGCAG 
GCCAAAGTTG GCATTGCGCC GCCCAATGTG GTGCTGGAAA GGAGTTTGTC CCTAAGCTCC 
ACAAATTCCA GCAGCTCCAT GAGCGGCAGT GAGAGTTCGC CGAATAGTTC ACCTTTGACG 
CAGGATATCA TACTCAATTC AGAACCGGCA GCAGCTCTGG CCGGAGCTGC TGCCTTGGGT 
GGCCCGGCGC CTGTCGATGT CACCGGTGGT CTGTCCACCG ATATCATTAT GAATCCCGCC 
GTTTCACCAT CGACTATTTT GTGCTCCGCA AATGGAGCGG CCACTGCCGT AGTGCCGAAT 
ATACTCGCAC CTCACCAAGT GACCATGGCC AACTCCATTT TGAACGATAT TGCCATGCAG 
CCGGAACCAA CACAGCAGGA TGCAGCAGTA GCAGCCTTGG CGTTGAGCAA CATCATGATG 
AGTCCACCCA CCGCGGCATC GGGTGTGGGA GTTGGTGACA CACTGCCACC AACACCAGCT 
ACTATGCAGC CAGAGGTCGC AGCCACTGCC ACATCCACTG CGGTCAGCAA CATGATTATT 
AAGGCGGCGG CGGACTTTAT AACCACCCAG GAGCAGGAGC AACACCACTA CCATCACCAC 
GGTCGCATCC ACTCCCATTC GCCACAGGCA GCTGTCGGAG TGAGCGGTAA TCCTGCCGAG 
ACGGCATCGG ATCCATTGGT TGACCTGCTA TTGAACCACT CAAACACACC AGAAACAGCG 
GCTGCTGCCG CTGCCGCAGT AGCTGTAGAA GCAGCCAACT TCCAGTCGAT GAATCACAGT 
CACCACAGCC ATCATGGTCA TCATGGTCAC AGTCACGGCC ACGGCCATAG TCGCGTCACA 
GGACACGTAT CCGGGCATGT GACCAGCCAC CATCGTGGTC ACCACTTGCC GGTGGTGCCG 
CCGACACCAC AAGAATCTCT GATCGTTGCG CTGGCCAGCG AGAATGCTCT GCAAAAGTCG 
GTGGCCACCG CCGCAGTGAC CACCAATGGA GCAGTGATGA CGCAACAGGC ATCTGCCCCG 
AACACAGCGG GTAGCATACT TCCGGCAGCG GTTGGAGCGG TAGCTGCGGC GGCCGCCGTG 
GCGGTGCAGC CGCCCATTCC CCAGGAGCTG ACCACGATGT CGGATCAGGA TCTGATCAGC 
TACATCAACC CGAGCACCTT TGATCAGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT ( NM_001169274.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162745.1 Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa03558
Clone ID Related Accession (Same CDS sequence)	NM_001169274.2 , NM_001298290.1
Accession Version	NM_001169274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NFAT nuclear factor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169274.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTCA CCTACAATCA GTATAAACAT TATGAGTCCG GTTATCGGAT TCCATCTAAA 
ATGCATAATC TGAGCCATAA TAACAACAAT AATAGTAATA ATAATAATAA TGGGGGTGGC 
CACACCAACA ACAACAACAA TGGCAACAAT AGTGGCCATC ACTATGCCCA TCACAATAAT 
AGCAACAATG GCGGCAATAA CAACACAAAT TTCCAAGCCT ATCAGCATCA TTACAAAAGT 
AATTTCGGCA TGAGAATGAC CATGTCCACC AATTCCACAA TGAGTGCGCG TATACATCGA 
AAAGGATTTC GCATACCATC GAAAAGACAG CCGGGTAAGG GATTGCCCGG CAAGTTGCAC 
ACCATAACCA GGACGGGTCC TGGCAAAATG GTTCCTGGGA AGCGAATACC ACAGCGACCC 
CATCCGCCGC CCTGCGACAA CTCGAACGAC AGTGGGCTGG GTTTCGATCA GCACACGGAG 
CTGAGATCCT CGGCAGGAGC TGGGGGCGTT ACTGATCCGG CGGCCAATGG AGGCAGTGGC 
TCCAACTCTG GCCAGCGATC CAGTTTGCTG GTCAATAGCG CTTTAACCAA CACAGTGGCT 
GCTGCAGCAG CCGCAGCAGC TGCCGCGGTG GCCAGCAACA CGTTGCAGCA GCATCAGCAG 
CACCATCAAC AGCAGCAGCA GCAACAGCAG CAACAACAGC AGCAGCAGCA GCAACAACAA 
CAACAGCAGC AGCAGCAACA ACATTCACCG CAGCAGCATT TAATCAGAGC GATACCTGTA 
TCAAGATTCA TATCGGCCAG GAATGTCACG CGTGTGGCGA ACAAACGTCA ACCCACTACA 
CCGCTGAACA GCGTCGCCAG CTCCAACGAT GGCCAGGTGC AGCTGGAGAT TTTGTCGCAG 
CCGGAGCAAC AGCACCGGGC ACGCTATCAA ACGGAGGGCA GCCGCGGTGC CGTCAAGGAT 
CGCAGCGGCA ATGGATTCCC GATCGTCCGA CTGACCGGAT ACGATAAGGT TGCCGTCCTC 
CAGGTCTTCA TCGGCACGGA CATTGGACGC GTGGCACCGC ACATGTTCTA TCAGGCCTGC 
AAGGTTGCCG GCAAGAACTC GACGCAGTGC AACGAGAAGA AGGTCGACGG CACCATGGTC 
ATTGAGATCG ATTTCAAGCC CGAAACGGAC ATGACCATCA CATGCGATTG CGTTGGAATC 
TTGAAGGAAC GCAATGTGGA TGTGGAGCAC CGATTTCCCG AGCATCTGGC GCAAAAGAAC 
AAGAAGAAAT CGACTCGCTG CCGGATGGTG TTCCGCACCC AGCTGACCCG TGACGATGGC 
ACCACCGAGA CCCTCCAGGT CTGCTCGAAT CCAATCATCT GCACTCAACC ACCTGGCGTG 
CCGGAAATAT GCAAGAAATC CTTAAACTCA TGTCCCGTTG ATGGCGGCCT CGAGCTTTTC 
ATTATCGGCA AGAACTTTCT GAAGGACACA CACGTGGTGT TTCAGGAGAC ATACGACAGC 
GTCAACGGTG ACGATCCGGC CACGGAAATT GCAGTGCGCC AGCAGCTCAT CGGCGGTACA 
GCCGCTCTTT GGGAACAGAG TGTTCTGCCG GATAAGGAGT ACCTTCACCA GACTCATCTG 
ATATGCACGG TGCCACCGTA TCTGCACCAG AACATCCTGA AACCGGTCCA GGTTCAAGTG 
TCGATCGTCT CCAGCGGCAA GAAGAGCGAA CCGCATACGT TCACCTACAC GGCCAAGGGA 
CAGTACACGA CGCTGGCGGC GGCCAGCACG TTAAGTAACA CAATCCACGC CCAAGATGTG 
AGCGGTTTCA TGGACACCAC ATCAGCGTCC AATGCGAGTG GCTCCAGTGG TTGGAGTGCG 
GCAGGCGGTT CCGGCAACAA TACATCTACA GGCGATAATG TGGAGGCCAA ACACGAGATC 
GATTCGGGCA TGATGCCTCC GCCCATTACC ACCCAAATAC CGATGGGCGT TCGACGCTCC 
TCGCTGCCCA GTGTCACACC CATGATCACG GACCAACAGC TGGTCCATCT CAACGCAGTG 
GCGGCCAGTG CCGAGGCCCT GAAGACGGAA CTCGACGACA GCAACTCGCA TAGCCCACTG 
ACCGGTGAAT CCACACCGGA TAGCCCCAAC GCAGCCTTGC AGTATCATCG CTTTGCACGA 
AAGCCCAGCC TGGACACCAT TATGTACGAT CAGTCGAACA GTCTGCCCGG TTTCCCGACC 
GTTGTGGCGC CGGCTACAGC TGCCGCCGTG GCCGCTGCAG TTGATATTGA TCCGACGGCG 
GTGGCCGTGG CGGTGGAACT GGCCGTAAAA AATGAGATTG TCAAGCATGT CGTACAGCAG 
CACCAGCAGA TGCAGGAGCA AATGCACGAG CAGCAGCAGC AGCAACAGCA GCAGCAACAA 
CAACAACAAC AGCAGCAACA ACAACAACCC CAACAGCAGC AGCAGCAACA GGTGCACAAG 
TTTATTGACG AGCTGACCAA ATCCACATCG GTGGTTTCCA GCAATGGTAC CACCGAACCA 
GCTCTGTTCA CCACCTCGGC GGTGATCGAT CATGCCCTGA CCGATATTCT GCAGGCCAAA 
GTTGGCATTG CGCCGCCCAA TGTGGTGCTG GAAAGGAGTT TGTCCCTAAG CTCCACAAAT 
TCCAGCAGCT CCATGAGCGG CAGTGAGAGT TCGCCGAATA GTTCACCTTT GACGCAGGAT 
ATCATACTCA ATTCAGAACC GGCAGCAGCT CTGGCCGGAG CTGCTGCCTT GGGTGGCCCG 
GCGCCTGTCG ATGTCACCGG TGGTCTGTCC ACCGATATCA TTATGAATCC CGCCGTTTCA 
CCATCGACTA TTTTGTGCTC CGCAAATGGA GCGGCCACTG CCGTAGTGCC GAATATACTC 
GCACCTCACC AAGTGACCAT GGCCAACTCC ATTTTGAACG ATATTGCCAT GCAGCCGGAA 
CCAACACAGC AGGATGCAGC AGTAGCAGCC TTGGCGTTGA GCAACATCAT GATGAGTCCA 
CCCACCGCGG CATCGGGTGT GGGAGTTGGT GACACACTGC CACCAACACC AGCTACTATG 
CAGCCAGAGG TCGCAGCCAC TGCCACATCC ACTGCGGTCA GCAACATGAT TATTAAGGCG 
GCGGCGGACT TTATAACCAC CCAGGAGCAG GAGCAACACC ACTACCATCA CCACGGTCGC 
ATCCACTCCC ATTCGCCACA GGCAGCTGTC GGAGTGAGCG GTAATCCTGC CGAGACGGCA 
TCGGATCCAT TGGTTGACCT GCTATTGAAC CACTCAAACA CACCAGAAAC AGCGGCTGCT 
GCCGCTGCCG CAGTAGCTGT AGAAGCAGCC AACTTCCAGT CGATGAATCA CAGTCACCAC 
AGCCATCATG GTCATCATGG TCACAGTCAC GGCCACGGCC ATAGTCGCGT CACAGGACAC 
GTATCCGGGC ATGTGACCAG CCACCATCGT GGTCACCACT TGCCGGTGGT GCCGCCGACA 
CCACAAGAAT CTCTGATCGT TGCGCTGGCC AGCGAGAATG CTCTGCAAAA GTCGGTGGCC 
ACCGCCGCAG TGACCACCAA TGGAGCAGTG ATGACGCAAC AGGCATCTGC CCCGAACACA 
GCGGGTAGCA TACTTCCGGC AGCGGTTGGA GCGGTAGCTG CGGCGGCCGC CGTGGCGGTG 
CAGCCGCCCA TTCCCCAGGA GCTGACCACG ATGTCGGATC AGGATCTGAT CAGCTACATC 
AACCCGAGCA CCTTTGATCA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162745.1
CDS	380..4126
Translation	MRFTYNQYKHYESGYRIPSKMHNLSHNNNNNSNNNNNGGGHTNNNNNGNNSGHHYAHHNNSNNGGNNNTNFQAYQHHYKSNFGMRMTMSTNSTMSARIHRKGFRIPSKRQPGKGLPGKLHTITRTGPGKMVPGKRIPQRPHPPPCDNSNDSGLGFDQHTELRSSAGAGGVTDPAANGGSGSNSGQRSSLLVNSALTNTVAAAAAAAAAAVASNTLQQHQQHHQQQQQQQQQQQQQQQQQQQQQQQQQHSPQQHLIRAIPVSRFISARNVTRVANKRQPTTPLNSVASSNDGQVQLEILSQPEQQHRARYQTEGSRGAVKDRSGNGFPIVRLTGYDKVAVLQVFIGTDIGRVAPHMFYQACKVAGKNSTQCNEKKVDGTMVIEIDFKPETDMTITCDCVGILKERNVDVEHRFPEHLAQKNKKKSTRCRMVFRTQLTRDDGTTETLQVCSNPIICTQPPGVPEICKKSLNSCPVDGGLELFIIGKNFLKDTHVVFQETYDSVNGDDPATEIAVRQQLIGGTAALWEQSVLPDKEYLHQTHLICTVPPYLHQNILKPVQVQVSIVSSGKKSEPHTFTYTAKGQYTTLAAASTLSNTIHAQDVSGFMDTTSASNASGSSGWSAAGGSGNNTSTGDNVEAKHEIDSGMMPPPITTQIPMGVRRSSLPSVTPMITDQQLVHLNAVAASAEALKTELDDSNSHSPLTGESTPDSPNAALQYHRFARKPSLDTIMYDQSNSLPGFPTVVAPATAAAVAAAVDIDPTAVAVAVELAVKNEIVKHVVQQHQQMQEQMHEQQQQQQQQQQQQQQQQQQQPQQQQQQQVHKFIDELTKSTSVVSSNGTTEPALFTTSAVIDHALTDILQAKVGIAPPNVVLERSLSLSSTNSSSSMSGSESSPNSSPLTQDIILNSEPAAALAGAAALGGPAPVDVTGGLSTDIIMNPAVSPSTILCSANGAATAVVPNILAPHQVTMANSILNDIAMQPEPTQQDAAVAALALSNIMMSPPTAASGVGVGDTLPPTPATMQPEVAATATSTAVSNMIIKAAADFITTQEQEQHHYHHHGRIHSHSPQAAVGVSGNPAETASDPLVDLLLNHSNTPETAAAAAAAVAVEAANFQSMNHSHHSHHGHHGHSHGHGHSRVTGHVSGHVTSHHRGHHLPVVPPTPQESLIVALASENALQKSVATAAVTTNGAVMTQQASAPNTAGSILPAAVGAVAAAAAVAVQPPIPQELTTMSDQDLISYINPSTFDQL

Target ORF information:

RefSeq Version	NM_001169274.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169274.2

ORF Insert Sequence:

ATGCGATTCA CCTACAATCA GTATAAACAT TATGAGTCCG GTTATCGGAT TCCATCTAAA 
ATGCATAATC TGAGCCATAA TAACAACAAT AATAGTAATA ATAATAATAA TGGGGGTGGC 
CACACCAACA ACAACAACAA TGGCAACAAT AGTGGCCATC ACTATGCCCA TCACAATAAT 
AGCAACAATG GCGGCAATAA CAACACAAAT TTCCAAGCCT ATCAGCATCA TTACAAAAGT 
AATTTCGGCA TGAGAATGAC CATGTCCACC AATTCCACAA TGAGTGCGCG TATACATCGA 
AAAGGATTTC GCATACCATC GAAAAGACAG CCGGGTAAGG GATTGCCCGG CAAGTTGCAC 
ACCATAACCA GGACGGGTCC TGGCAAAATG GTTCCTGGGA AGCGAATACC ACAGCGACCC 
CATCCGCCGC CCTGCGACAA CTCGAACGAC AGTGGGCTGG GTTTCGATCA GCACACGGAG 
CTGAGATCCT CGGCAGGAGC TGGGGGCGTT ACTGATCCGG CGGCCAATGG AGGCAGTGGC 
TCCAACTCTG GCCAGCGATC CAGTTTGCTG GTCAATAGCG CTTTAACCAA CACAGTGGCT 
GCTGCAGCAG CCGCAGCAGC TGCCGCGGTG GCCAGCAACA CGTTGCAGCA GCATCAGCAG 
CACCATCAAC AGCAGCAGCA GCAACAGCAG CAACAACAGC AGCAGCAGCA GCAACAACAA 
CAACAGCAGC AGCAGCAACA ACATTCACCG CAGCAGCATT TAATCAGAGC GATACCTGTA 
TCAAGATTCA TATCGGCCAG GAATGTCACG CGTGTGGCGA ACAAACGTCA ACCCACTACA 
CCGCTGAACA GCGTCGCCAG CTCCAACGAT GGCCAGGTGC AGCTGGAGAT TTTGTCGCAG 
CCGGAGCAAC AGCACCGGGC ACGCTATCAA ACGGAGGGCA GCCGCGGTGC CGTCAAGGAT 
CGCAGCGGCA ATGGATTCCC GATCGTCCGA CTGACCGGAT ACGATAAGGT TGCCGTCCTC 
CAGGTCTTCA TCGGCACGGA CATTGGACGC GTGGCACCGC ACATGTTCTA TCAGGCCTGC 
AAGGTTGCCG GCAAGAACTC GACGCAGTGC AACGAGAAGA AGGTCGACGG CACCATGGTC 
ATTGAGATCG ATTTCAAGCC CGAAACGGAC ATGACCATCA CATGCGATTG CGTTGGAATC 
TTGAAGGAAC GCAATGTGGA TGTGGAGCAC CGATTTCCCG AGCATCTGGC GCAAAAGAAC 
AAGAAGAAAT CGACTCGCTG CCGGATGGTG TTCCGCACCC AGCTGACCCG TGACGATGGC 
ACCACCGAGA CCCTCCAGGT CTGCTCGAAT CCAATCATCT GCACTCAACC ACCTGGCGTG 
CCGGAAATAT GCAAGAAATC CTTAAACTCA TGTCCCGTTG ATGGCGGCCT CGAGCTTTTC 
ATTATCGGCA AGAACTTTCT GAAGGACACA CACGTGGTGT TTCAGGAGAC ATACGACAGC 
GTCAACGGTG ACGATCCGGC CACGGAAATT GCAGTGCGCC AGCAGCTCAT CGGCGGTACA 
GCCGCTCTTT GGGAACAGAG TGTTCTGCCG GATAAGGAGT ACCTTCACCA GACTCATCTG 
ATATGCACGG TGCCACCGTA TCTGCACCAG AACATCCTGA AACCGGTCCA GGTTCAAGTG 
TCGATCGTCT CCAGCGGCAA GAAGAGCGAA CCGCATACGT TCACCTACAC GGCCAAGGGA 
CAGTACACGA CGCTGGCGGC GGCCAGCACG TTAAGTAACA CAATCCACGC CCAAGATGTG 
AGCGGTTTCA TGGACACCAC ATCAGCGTCC AATGCGAGTG GCTCCAGTGG TTGGAGTGCG 
GCAGGCGGTT CCGGCAACAA TACATCTACA GGCGATAATG TGGAGGCCAA ACACGAGATC 
GATTCGGGCA TGATGCCTCC GCCCATTACC ACCCAAATAC CGATGGGCGT TCGACGCTCC 
TCGCTGCCCA GTGTCACACC CATGATCACG GACCAACAGC TGGTCCATCT CAACGCAGTG 
GCGGCCAGTG CCGAGGCCCT GAAGACGGAA CTCGACGACA GCAACTCGCA TAGCCCACTG 
ACCGGTGAAT CCACACCGGA TAGCCCCAAC GCAGCCTTGC AGTATCATCG CTTTGCACGA 
AAGCCCAGCC TGGACACCAT TATGTACGAT CAGTCGAACA GTCTGCCCGG TTTCCCGACC 
GTTGTGGCGC CGGCTACAGC TGCCGCCGTG GCCGCTGCAG TTGATATTGA TCCGACGGCG 
GTGGCCGTGG CGGTGGAACT GGCCGTAAAA AATGAGATTG TCAAGCATGT CGTACAGCAG 
CACCAGCAGA TGCAGGAGCA AATGCACGAG CAGCAGCAGC AGCAACAGCA GCAGCAACAA 
CAACAACAAC AGCAGCAACA ACAACAACCC CAACAGCAGC AGCAGCAACA GGTGCACAAG 
TTTATTGACG AGCTGACCAA ATCCACATCG GTGGTTTCCA GCAATGGTAC CACCGAACCA 
GCTCTGTTCA CCACCTCGGC GGTGATCGAT CATGCCCTGA CCGATATTCT GCAGGCCAAA 
GTTGGCATTG CGCCGCCCAA TGTGGTGCTG GAAAGGAGTT TGTCCCTAAG CTCCACAAAT 
TCCAGCAGCT CCATGAGCGG CAGTGAGAGT TCGCCGAATA GTTCACCTTT GACGCAGGAT 
ATCATACTCA ATTCAGAACC GGCAGCAGCT CTGGCCGGAG CTGCTGCCTT GGGTGGCCCG 
GCGCCTGTCG ATGTCACCGG TGGTCTGTCC ACCGATATCA TTATGAATCC CGCCGTTTCA 
CCATCGACTA TTTTGTGCTC CGCAAATGGA GCGGCCACTG CCGTAGTGCC GAATATACTC 
GCACCTCACC AAGTGACCAT GGCCAACTCC ATTTTGAACG ATATTGCCAT GCAGCCGGAA 
CCAACACAGC AGGATGCAGC AGTAGCAGCC TTGGCGTTGA GCAACATCAT GATGAGTCCA 
CCCACCGCGG CATCGGGTGT GGGAGTTGGT GACACACTGC CACCAACACC AGCTACTATG 
CAGCCAGAGG TCGCAGCCAC TGCCACATCC ACTGCGGTCA GCAACATGAT TATTAAGGCG 
GCGGCGGACT TTATAACCAC CCAGGAGCAG GAGCAACACC ACTACCATCA CCACGGTCGC 
ATCCACTCCC ATTCGCCACA GGCAGCTGTC GGAGTGAGCG GTAATCCTGC CGAGACGGCA 
TCGGATCCAT TGGTTGACCT GCTATTGAAC CACTCAAACA CACCAGAAAC AGCGGCTGCT 
GCCGCTGCCG CAGTAGCTGT AGAAGCAGCC AACTTCCAGT CGATGAATCA CAGTCACCAC 
AGCCATCATG GTCATCATGG TCACAGTCAC GGCCACGGCC ATAGTCGCGT CACAGGACAC 
GTATCCGGGC ATGTGACCAG CCACCATCGT GGTCACCACT TGCCGGTGGT GCCGCCGACA 
CCACAAGAAT CTCTGATCGT TGCGCTGGCC AGCGAGAATG CTCTGCAAAA GTCGGTGGCC 
ACCGCCGCAG TGACCACCAA TGGAGCAGTG ATGACGCAAC AGGCATCTGC CCCGAACACA 
GCGGGTAGCA TACTTCCGGC AGCGGTTGGA GCGGTAGCTG CGGCGGCCGC CGTGGCGGTG 
CAGCCGCCCA TTCCCCAGGA GCTGACCACG ATGTCGGATC AGGATCTGAT CAGCTACATC 
AACCCGAGCA CCTTTGATCA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT ( NM_001298290.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285219.1 Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant H, mRNA.

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CloneID	OFa03558
Clone ID Related Accession (Same CDS sequence)	NM_001169274.2 , NM_001298290.1
Accession Version	NM_001298290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NFAT nuclear factor, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTCA CCTACAATCA GTATAAACAT TATGAGTCCG GTTATCGGAT TCCATCTAAA 
ATGCATAATC TGAGCCATAA TAACAACAAT AATAGTAATA ATAATAATAA TGGGGGTGGC 
CACACCAACA ACAACAACAA TGGCAACAAT AGTGGCCATC ACTATGCCCA TCACAATAAT 
AGCAACAATG GCGGCAATAA CAACACAAAT TTCCAAGCCT ATCAGCATCA TTACAAAAGT 
AATTTCGGCA TGAGAATGAC CATGTCCACC AATTCCACAA TGAGTGCGCG TATACATCGA 
AAAGGATTTC GCATACCATC GAAAAGACAG CCGGGTAAGG GATTGCCCGG CAAGTTGCAC 
ACCATAACCA GGACGGGTCC TGGCAAAATG GTTCCTGGGA AGCGAATACC ACAGCGACCC 
CATCCGCCGC CCTGCGACAA CTCGAACGAC AGTGGGCTGG GTTTCGATCA GCACACGGAG 
CTGAGATCCT CGGCAGGAGC TGGGGGCGTT ACTGATCCGG CGGCCAATGG AGGCAGTGGC 
TCCAACTCTG GCCAGCGATC CAGTTTGCTG GTCAATAGCG CTTTAACCAA CACAGTGGCT 
GCTGCAGCAG CCGCAGCAGC TGCCGCGGTG GCCAGCAACA CGTTGCAGCA GCATCAGCAG 
CACCATCAAC AGCAGCAGCA GCAACAGCAG CAACAACAGC AGCAGCAGCA GCAACAACAA 
CAACAGCAGC AGCAGCAACA ACATTCACCG CAGCAGCATT TAATCAGAGC GATACCTGTA 
TCAAGATTCA TATCGGCCAG GAATGTCACG CGTGTGGCGA ACAAACGTCA ACCCACTACA 
CCGCTGAACA GCGTCGCCAG CTCCAACGAT GGCCAGGTGC AGCTGGAGAT TTTGTCGCAG 
CCGGAGCAAC AGCACCGGGC ACGCTATCAA ACGGAGGGCA GCCGCGGTGC CGTCAAGGAT 
CGCAGCGGCA ATGGATTCCC GATCGTCCGA CTGACCGGAT ACGATAAGGT TGCCGTCCTC 
CAGGTCTTCA TCGGCACGGA CATTGGACGC GTGGCACCGC ACATGTTCTA TCAGGCCTGC 
AAGGTTGCCG GCAAGAACTC GACGCAGTGC AACGAGAAGA AGGTCGACGG CACCATGGTC 
ATTGAGATCG ATTTCAAGCC CGAAACGGAC ATGACCATCA CATGCGATTG CGTTGGAATC 
TTGAAGGAAC GCAATGTGGA TGTGGAGCAC CGATTTCCCG AGCATCTGGC GCAAAAGAAC 
AAGAAGAAAT CGACTCGCTG CCGGATGGTG TTCCGCACCC AGCTGACCCG TGACGATGGC 
ACCACCGAGA CCCTCCAGGT CTGCTCGAAT CCAATCATCT GCACTCAACC ACCTGGCGTG 
CCGGAAATAT GCAAGAAATC CTTAAACTCA TGTCCCGTTG ATGGCGGCCT CGAGCTTTTC 
ATTATCGGCA AGAACTTTCT GAAGGACACA CACGTGGTGT TTCAGGAGAC ATACGACAGC 
GTCAACGGTG ACGATCCGGC CACGGAAATT GCAGTGCGCC AGCAGCTCAT CGGCGGTACA 
GCCGCTCTTT GGGAACAGAG TGTTCTGCCG GATAAGGAGT ACCTTCACCA GACTCATCTG 
ATATGCACGG TGCCACCGTA TCTGCACCAG AACATCCTGA AACCGGTCCA GGTTCAAGTG 
TCGATCGTCT CCAGCGGCAA GAAGAGCGAA CCGCATACGT TCACCTACAC GGCCAAGGGA 
CAGTACACGA CGCTGGCGGC GGCCAGCACG TTAAGTAACA CAATCCACGC CCAAGATGTG 
AGCGGTTTCA TGGACACCAC ATCAGCGTCC AATGCGAGTG GCTCCAGTGG TTGGAGTGCG 
GCAGGCGGTT CCGGCAACAA TACATCTACA GGCGATAATG TGGAGGCCAA ACACGAGATC 
GATTCGGGCA TGATGCCTCC GCCCATTACC ACCCAAATAC CGATGGGCGT TCGACGCTCC 
TCGCTGCCCA GTGTCACACC CATGATCACG GACCAACAGC TGGTCCATCT CAACGCAGTG 
GCGGCCAGTG CCGAGGCCCT GAAGACGGAA CTCGACGACA GCAACTCGCA TAGCCCACTG 
ACCGGTGAAT CCACACCGGA TAGCCCCAAC GCAGCCTTGC AGTATCATCG CTTTGCACGA 
AAGCCCAGCC TGGACACCAT TATGTACGAT CAGTCGAACA GTCTGCCCGG TTTCCCGACC 
GTTGTGGCGC CGGCTACAGC TGCCGCCGTG GCCGCTGCAG TTGATATTGA TCCGACGGCG 
GTGGCCGTGG CGGTGGAACT GGCCGTAAAA AATGAGATTG TCAAGCATGT CGTACAGCAG 
CACCAGCAGA TGCAGGAGCA AATGCACGAG CAGCAGCAGC AGCAACAGCA GCAGCAACAA 
CAACAACAAC AGCAGCAACA ACAACAACCC CAACAGCAGC AGCAGCAACA GGTGCACAAG 
TTTATTGACG AGCTGACCAA ATCCACATCG GTGGTTTCCA GCAATGGTAC CACCGAACCA 
GCTCTGTTCA CCACCTCGGC GGTGATCGAT CATGCCCTGA CCGATATTCT GCAGGCCAAA 
GTTGGCATTG CGCCGCCCAA TGTGGTGCTG GAAAGGAGTT TGTCCCTAAG CTCCACAAAT 
TCCAGCAGCT CCATGAGCGG CAGTGAGAGT TCGCCGAATA GTTCACCTTT GACGCAGGAT 
ATCATACTCA ATTCAGAACC GGCAGCAGCT CTGGCCGGAG CTGCTGCCTT GGGTGGCCCG 
GCGCCTGTCG ATGTCACCGG TGGTCTGTCC ACCGATATCA TTATGAATCC CGCCGTTTCA 
CCATCGACTA TTTTGTGCTC CGCAAATGGA GCGGCCACTG CCGTAGTGCC GAATATACTC 
GCACCTCACC AAGTGACCAT GGCCAACTCC ATTTTGAACG ATATTGCCAT GCAGCCGGAA 
CCAACACAGC AGGATGCAGC AGTAGCAGCC TTGGCGTTGA GCAACATCAT GATGAGTCCA 
CCCACCGCGG CATCGGGTGT GGGAGTTGGT GACACACTGC CACCAACACC AGCTACTATG 
CAGCCAGAGG TCGCAGCCAC TGCCACATCC ACTGCGGTCA GCAACATGAT TATTAAGGCG 
GCGGCGGACT TTATAACCAC CCAGGAGCAG GAGCAACACC ACTACCATCA CCACGGTCGC 
ATCCACTCCC ATTCGCCACA GGCAGCTGTC GGAGTGAGCG GTAATCCTGC CGAGACGGCA 
TCGGATCCAT TGGTTGACCT GCTATTGAAC CACTCAAACA CACCAGAAAC AGCGGCTGCT 
GCCGCTGCCG CAGTAGCTGT AGAAGCAGCC AACTTCCAGT CGATGAATCA CAGTCACCAC 
AGCCATCATG GTCATCATGG TCACAGTCAC GGCCACGGCC ATAGTCGCGT CACAGGACAC 
GTATCCGGGC ATGTGACCAG CCACCATCGT GGTCACCACT TGCCGGTGGT GCCGCCGACA 
CCACAAGAAT CTCTGATCGT TGCGCTGGCC AGCGAGAATG CTCTGCAAAA GTCGGTGGCC 
ACCGCCGCAG TGACCACCAA TGGAGCAGTG ATGACGCAAC AGGCATCTGC CCCGAACACA 
GCGGGTAGCA TACTTCCGGC AGCGGTTGGA GCGGTAGCTG CGGCGGCCGC CGTGGCGGTG 
CAGCCGCCCA TTCCCCAGGA GCTGACCACG ATGTCGGATC AGGATCTGAT CAGCTACATC 
AACCCGAGCA CCTTTGATCA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285219.1
CDS	405..4151
Translation	MRFTYNQYKHYESGYRIPSKMHNLSHNNNNNSNNNNNGGGHTNNNNNGNNSGHHYAHHNNSNNGGNNNTNFQAYQHHYKSNFGMRMTMSTNSTMSARIHRKGFRIPSKRQPGKGLPGKLHTITRTGPGKMVPGKRIPQRPHPPPCDNSNDSGLGFDQHTELRSSAGAGGVTDPAANGGSGSNSGQRSSLLVNSALTNTVAAAAAAAAAAVASNTLQQHQQHHQQQQQQQQQQQQQQQQQQQQQQQQQHSPQQHLIRAIPVSRFISARNVTRVANKRQPTTPLNSVASSNDGQVQLEILSQPEQQHRARYQTEGSRGAVKDRSGNGFPIVRLTGYDKVAVLQVFIGTDIGRVAPHMFYQACKVAGKNSTQCNEKKVDGTMVIEIDFKPETDMTITCDCVGILKERNVDVEHRFPEHLAQKNKKKSTRCRMVFRTQLTRDDGTTETLQVCSNPIICTQPPGVPEICKKSLNSCPVDGGLELFIIGKNFLKDTHVVFQETYDSVNGDDPATEIAVRQQLIGGTAALWEQSVLPDKEYLHQTHLICTVPPYLHQNILKPVQVQVSIVSSGKKSEPHTFTYTAKGQYTTLAAASTLSNTIHAQDVSGFMDTTSASNASGSSGWSAAGGSGNNTSTGDNVEAKHEIDSGMMPPPITTQIPMGVRRSSLPSVTPMITDQQLVHLNAVAASAEALKTELDDSNSHSPLTGESTPDSPNAALQYHRFARKPSLDTIMYDQSNSLPGFPTVVAPATAAAVAAAVDIDPTAVAVAVELAVKNEIVKHVVQQHQQMQEQMHEQQQQQQQQQQQQQQQQQQQPQQQQQQQVHKFIDELTKSTSVVSSNGTTEPALFTTSAVIDHALTDILQAKVGIAPPNVVLERSLSLSSTNSSSSMSGSESSPNSSPLTQDIILNSEPAAALAGAAALGGPAPVDVTGGLSTDIIMNPAVSPSTILCSANGAATAVVPNILAPHQVTMANSILNDIAMQPEPTQQDAAVAALALSNIMMSPPTAASGVGVGDTLPPTPATMQPEVAATATSTAVSNMIIKAAADFITTQEQEQHHYHHHGRIHSHSPQAAVGVSGNPAETASDPLVDLLLNHSNTPETAAAAAAAVAVEAANFQSMNHSHHSHHGHHGHSHGHGHSRVTGHVSGHVTSHHRGHHLPVVPPTPQESLIVALASENALQKSVATAAVTTNGAVMTQQASAPNTAGSILPAAVGAVAAAAAVAVQPPIPQELTTMSDQDLISYINPSTFDQL

Target ORF information:

RefSeq Version	NM_001298290.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298290.1

ORF Insert Sequence:

ATGCGATTCA CCTACAATCA GTATAAACAT TATGAGTCCG GTTATCGGAT TCCATCTAAA 
ATGCATAATC TGAGCCATAA TAACAACAAT AATAGTAATA ATAATAATAA TGGGGGTGGC 
CACACCAACA ACAACAACAA TGGCAACAAT AGTGGCCATC ACTATGCCCA TCACAATAAT 
AGCAACAATG GCGGCAATAA CAACACAAAT TTCCAAGCCT ATCAGCATCA TTACAAAAGT 
AATTTCGGCA TGAGAATGAC CATGTCCACC AATTCCACAA TGAGTGCGCG TATACATCGA 
AAAGGATTTC GCATACCATC GAAAAGACAG CCGGGTAAGG GATTGCCCGG CAAGTTGCAC 
ACCATAACCA GGACGGGTCC TGGCAAAATG GTTCCTGGGA AGCGAATACC ACAGCGACCC 
CATCCGCCGC CCTGCGACAA CTCGAACGAC AGTGGGCTGG GTTTCGATCA GCACACGGAG 
CTGAGATCCT CGGCAGGAGC TGGGGGCGTT ACTGATCCGG CGGCCAATGG AGGCAGTGGC 
TCCAACTCTG GCCAGCGATC CAGTTTGCTG GTCAATAGCG CTTTAACCAA CACAGTGGCT 
GCTGCAGCAG CCGCAGCAGC TGCCGCGGTG GCCAGCAACA CGTTGCAGCA GCATCAGCAG 
CACCATCAAC AGCAGCAGCA GCAACAGCAG CAACAACAGC AGCAGCAGCA GCAACAACAA 
CAACAGCAGC AGCAGCAACA ACATTCACCG CAGCAGCATT TAATCAGAGC GATACCTGTA 
TCAAGATTCA TATCGGCCAG GAATGTCACG CGTGTGGCGA ACAAACGTCA ACCCACTACA 
CCGCTGAACA GCGTCGCCAG CTCCAACGAT GGCCAGGTGC AGCTGGAGAT TTTGTCGCAG 
CCGGAGCAAC AGCACCGGGC ACGCTATCAA ACGGAGGGCA GCCGCGGTGC CGTCAAGGAT 
CGCAGCGGCA ATGGATTCCC GATCGTCCGA CTGACCGGAT ACGATAAGGT TGCCGTCCTC 
CAGGTCTTCA TCGGCACGGA CATTGGACGC GTGGCACCGC ACATGTTCTA TCAGGCCTGC 
AAGGTTGCCG GCAAGAACTC GACGCAGTGC AACGAGAAGA AGGTCGACGG CACCATGGTC 
ATTGAGATCG ATTTCAAGCC CGAAACGGAC ATGACCATCA CATGCGATTG CGTTGGAATC 
TTGAAGGAAC GCAATGTGGA TGTGGAGCAC CGATTTCCCG AGCATCTGGC GCAAAAGAAC 
AAGAAGAAAT CGACTCGCTG CCGGATGGTG TTCCGCACCC AGCTGACCCG TGACGATGGC 
ACCACCGAGA CCCTCCAGGT CTGCTCGAAT CCAATCATCT GCACTCAACC ACCTGGCGTG 
CCGGAAATAT GCAAGAAATC CTTAAACTCA TGTCCCGTTG ATGGCGGCCT CGAGCTTTTC 
ATTATCGGCA AGAACTTTCT GAAGGACACA CACGTGGTGT TTCAGGAGAC ATACGACAGC 
GTCAACGGTG ACGATCCGGC CACGGAAATT GCAGTGCGCC AGCAGCTCAT CGGCGGTACA 
GCCGCTCTTT GGGAACAGAG TGTTCTGCCG GATAAGGAGT ACCTTCACCA GACTCATCTG 
ATATGCACGG TGCCACCGTA TCTGCACCAG AACATCCTGA AACCGGTCCA GGTTCAAGTG 
TCGATCGTCT CCAGCGGCAA GAAGAGCGAA CCGCATACGT TCACCTACAC GGCCAAGGGA 
CAGTACACGA CGCTGGCGGC GGCCAGCACG TTAAGTAACA CAATCCACGC CCAAGATGTG 
AGCGGTTTCA TGGACACCAC ATCAGCGTCC AATGCGAGTG GCTCCAGTGG TTGGAGTGCG 
GCAGGCGGTT CCGGCAACAA TACATCTACA GGCGATAATG TGGAGGCCAA ACACGAGATC 
GATTCGGGCA TGATGCCTCC GCCCATTACC ACCCAAATAC CGATGGGCGT TCGACGCTCC 
TCGCTGCCCA GTGTCACACC CATGATCACG GACCAACAGC TGGTCCATCT CAACGCAGTG 
GCGGCCAGTG CCGAGGCCCT GAAGACGGAA CTCGACGACA GCAACTCGCA TAGCCCACTG 
ACCGGTGAAT CCACACCGGA TAGCCCCAAC GCAGCCTTGC AGTATCATCG CTTTGCACGA 
AAGCCCAGCC TGGACACCAT TATGTACGAT CAGTCGAACA GTCTGCCCGG TTTCCCGACC 
GTTGTGGCGC CGGCTACAGC TGCCGCCGTG GCCGCTGCAG TTGATATTGA TCCGACGGCG 
GTGGCCGTGG CGGTGGAACT GGCCGTAAAA AATGAGATTG TCAAGCATGT CGTACAGCAG 
CACCAGCAGA TGCAGGAGCA AATGCACGAG CAGCAGCAGC AGCAACAGCA GCAGCAACAA 
CAACAACAAC AGCAGCAACA ACAACAACCC CAACAGCAGC AGCAGCAACA GGTGCACAAG 
TTTATTGACG AGCTGACCAA ATCCACATCG GTGGTTTCCA GCAATGGTAC CACCGAACCA 
GCTCTGTTCA CCACCTCGGC GGTGATCGAT CATGCCCTGA CCGATATTCT GCAGGCCAAA 
GTTGGCATTG CGCCGCCCAA TGTGGTGCTG GAAAGGAGTT TGTCCCTAAG CTCCACAAAT 
TCCAGCAGCT CCATGAGCGG CAGTGAGAGT TCGCCGAATA GTTCACCTTT GACGCAGGAT 
ATCATACTCA ATTCAGAACC GGCAGCAGCT CTGGCCGGAG CTGCTGCCTT GGGTGGCCCG 
GCGCCTGTCG ATGTCACCGG TGGTCTGTCC ACCGATATCA TTATGAATCC CGCCGTTTCA 
CCATCGACTA TTTTGTGCTC CGCAAATGGA GCGGCCACTG CCGTAGTGCC GAATATACTC 
GCACCTCACC AAGTGACCAT GGCCAACTCC ATTTTGAACG ATATTGCCAT GCAGCCGGAA 
CCAACACAGC AGGATGCAGC AGTAGCAGCC TTGGCGTTGA GCAACATCAT GATGAGTCCA 
CCCACCGCGG CATCGGGTGT GGGAGTTGGT GACACACTGC CACCAACACC AGCTACTATG 
CAGCCAGAGG TCGCAGCCAC TGCCACATCC ACTGCGGTCA GCAACATGAT TATTAAGGCG 
GCGGCGGACT TTATAACCAC CCAGGAGCAG GAGCAACACC ACTACCATCA CCACGGTCGC 
ATCCACTCCC ATTCGCCACA GGCAGCTGTC GGAGTGAGCG GTAATCCTGC CGAGACGGCA 
TCGGATCCAT TGGTTGACCT GCTATTGAAC CACTCAAACA CACCAGAAAC AGCGGCTGCT 
GCCGCTGCCG CAGTAGCTGT AGAAGCAGCC AACTTCCAGT CGATGAATCA CAGTCACCAC 
AGCCATCATG GTCATCATGG TCACAGTCAC GGCCACGGCC ATAGTCGCGT CACAGGACAC 
GTATCCGGGC ATGTGACCAG CCACCATCGT GGTCACCACT TGCCGGTGGT GCCGCCGACA 
CCACAAGAAT CTCTGATCGT TGCGCTGGCC AGCGAGAATG CTCTGCAAAA GTCGGTGGCC 
ACCGCCGCAG TGACCACCAA TGGAGCAGTG ATGACGCAAC AGGCATCTGC CCCGAACACA 
GCGGGTAGCA TACTTCCGGC AGCGGTTGGA GCGGTAGCTG CGGCGGCCGC CGTGGCGGTG 
CAGCCGCCCA TTCCCCAGGA GCTGACCACG ATGTCGGATC AGGATCTGAT CAGCTACATC 
AACCCGAGCA CCTTTGATCA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFAT ( NM_001272597.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259526.2 Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa21461
Clone ID Related Accession (Same CDS sequence)	NM_001272597.2
Accession Version	NM_001272597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NFAT nuclear factor, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272597.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTTTGA AATTCACAAA AACCAGTGGC GCTGGACCCT CGGCCGTACC GCTACTGACC 
ACCGCCCCCG CGACAGTCAC CAGTTCGGCG TCCATGCAGG CAACAACAAC AAGTTACGCG 
GCCCGTGGAA CAGGCATGAG AATGACCATG TCCACCAATT CCACAATGAG TGCGCGTATA 
CATCGAAAAG GATTTCGCAT ACCATCGAAA AGACAGCCGG GTAAGGGATT GCCCGGCAAG 
TTGCACACCA TAACCAGGAC GGGTCCTGGC AAAATGGTTC CTGGGAAGCG AATACCACAG 
CGACCCCATC CGCCGCCCTG CGACAACTCG AACGACAGTG GGCTGGGTTT CGATCAGCAC 
ACGGAGCTGA GATCCTCGGC AGGAGCTGGG GGCGTTACTG ATCCGGCGGC CAATGGAGGC 
AGTGGCTCCA ACTCTGGCCA GCGATCCAGT TTGCTGGTCA ATAGCGCTTT AACCAACACA 
GTGGCTGCTG CAGCAGCCGC AGCAGCTGCC GCGGTGGCCA GCAACACGTT GCAGCAGCAT 
CAGCAGCACC ATCAACAGCA GCAGCAGCAA CAGCAGCAAC AACAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAGCAGCA GCAACAACAT TCACCGCAGC AGCATTTAAT CAGAGCGATA 
CCTGTATCAA GATTCATATC GGCCAGGAAT GTCACGCGTG TGGCGAACAA ACGTCAACCC 
ACTACACCGC TGAACAGCGT CGCCAGCTCC AACGATGGCC AGGTGCAGCT GGAGATTTTG 
TCGCAGCCGG AGCAACAGCA CCGGGCACGC TATCAAACGG AGGGCAGCCG CGGTGCCGTC 
AAGGATCGCA GCGGCAATGG ATTCCCGATC GTCCGACTGA CCGGATACGA TAAGGTTGCC 
GTCCTCCAGG TCTTCATCGG CACGGACATT GGACGCGTGG CACCGCACAT GTTCTATCAG 
GCCTGCAAGG TTGCCGGCAA GAACTCGACG CAGTGCAACG AGAAGAAGGT CGACGGCACC 
ATGGTCATTG AGATCGATTT CAAGCCCGAA ACGGACATGA CCATCACATG CGATTGCGTT 
GGAATCTTGA AGGAACGCAA TGTGGATGTG GAGCACCGAT TTCCCGAGCA TCTGGCGCAA 
AAGAACAAGA AGAAATCGAC TCGCTGCCGG ATGGTGTTCC GCACCCAGCT GACCCGTGAC 
GATGGCACCA CCGAGACCCT CCAGGTCTGC TCGAATCCAA TCATCTGCAC TCAACCACCT 
GGCGTGCCGG AAATATGCAA GAAATCCTTA AACTCATGTC CCGTTGATGG CGGCCTCGAG 
CTTTTCATTA TCGGCAAGAA CTTTCTGAAG GACACACACG TGGTGTTTCA GGAGACATAC 
GACAGCGTCA ACGGTGACGA TCCGGCCACG GAAATTGCAG TGCGCCAGCA GCTCATCGGC 
GGTACAGCCG CTCTTTGGGA ACAGAGTGTT CTGCCGGATA AGGAGTACCT TCACCAGACT 
CATCTGATAT GCACGGTGCC ACCGTATCTG CACCAGAACA TCCTGAAACC GGTCCAGGTT 
CAAGTGTCGA TCGTCTCCAG CGGCAAGAAG AGCGAACCGC ATACGTTCAC CTACACGGCC 
AAGGGACAGT ACACGACGCT GGCGGCGGCC AGCACGTTAA GTAACACAAT CCACGCCCAA 
GCAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259526.2
CDS	713..2401
Translation	MLLKFTKTSGAGPSAVPLLTTAPATVTSSASMQATTTSYAARGTGMRMTMSTNSTMSARIHRKGFRIPSKRQPGKGLPGKLHTITRTGPGKMVPGKRIPQRPHPPPCDNSNDSGLGFDQHTELRSSAGAGGVTDPAANGGSGSNSGQRSSLLVNSALTNTVAAAAAAAAAAVASNTLQQHQQHHQQQQQQQQQQQQQQQQQQQQQQQQQHSPQQHLIRAIPVSRFISARNVTRVANKRQPTTPLNSVASSNDGQVQLEILSQPEQQHRARYQTEGSRGAVKDRSGNGFPIVRLTGYDKVAVLQVFIGTDIGRVAPHMFYQACKVAGKNSTQCNEKKVDGTMVIEIDFKPETDMTITCDCVGILKERNVDVEHRFPEHLAQKNKKKSTRCRMVFRTQLTRDDGTTETLQVCSNPIICTQPPGVPEICKKSLNSCPVDGGLELFIIGKNFLKDTHVVFQETYDSVNGDDPATEIAVRQQLIGGTAALWEQSVLPDKEYLHQTHLICTVPPYLHQNILKPVQVQVSIVSSGKKSEPHTFTYTAKGQYTTLAAASTLSNTIHAQAI

Target ORF information:

RefSeq Version	NM_001272597.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NFAT nuclear factor (NFAT), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272597.2

ORF Insert Sequence:

ATGCTTTTGA AATTCACAAA AACCAGTGGC GCTGGACCCT CGGCCGTACC GCTACTGACC 
ACCGCCCCCG CGACAGTCAC CAGTTCGGCG TCCATGCAGG CAACAACAAC AAGTTACGCG 
GCCCGTGGAA CAGGCATGAG AATGACCATG TCCACCAATT CCACAATGAG TGCGCGTATA 
CATCGAAAAG GATTTCGCAT ACCATCGAAA AGACAGCCGG GTAAGGGATT GCCCGGCAAG 
TTGCACACCA TAACCAGGAC GGGTCCTGGC AAAATGGTTC CTGGGAAGCG AATACCACAG 
CGACCCCATC CGCCGCCCTG CGACAACTCG AACGACAGTG GGCTGGGTTT CGATCAGCAC 
ACGGAGCTGA GATCCTCGGC AGGAGCTGGG GGCGTTACTG ATCCGGCGGC CAATGGAGGC 
AGTGGCTCCA ACTCTGGCCA GCGATCCAGT TTGCTGGTCA ATAGCGCTTT AACCAACACA 
GTGGCTGCTG CAGCAGCCGC AGCAGCTGCC GCGGTGGCCA GCAACACGTT GCAGCAGCAT 
CAGCAGCACC ATCAACAGCA GCAGCAGCAA CAGCAGCAAC AACAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAGCAGCA GCAACAACAT TCACCGCAGC AGCATTTAAT CAGAGCGATA 
CCTGTATCAA GATTCATATC GGCCAGGAAT GTCACGCGTG TGGCGAACAA ACGTCAACCC 
ACTACACCGC TGAACAGCGT CGCCAGCTCC AACGATGGCC AGGTGCAGCT GGAGATTTTG 
TCGCAGCCGG AGCAACAGCA CCGGGCACGC TATCAAACGG AGGGCAGCCG CGGTGCCGTC 
AAGGATCGCA GCGGCAATGG ATTCCCGATC GTCCGACTGA CCGGATACGA TAAGGTTGCC 
GTCCTCCAGG TCTTCATCGG CACGGACATT GGACGCGTGG CACCGCACAT GTTCTATCAG 
GCCTGCAAGG TTGCCGGCAA GAACTCGACG CAGTGCAACG AGAAGAAGGT CGACGGCACC 
ATGGTCATTG AGATCGATTT CAAGCCCGAA ACGGACATGA CCATCACATG CGATTGCGTT 
GGAATCTTGA AGGAACGCAA TGTGGATGTG GAGCACCGAT TTCCCGAGCA TCTGGCGCAA 
AAGAACAAGA AGAAATCGAC TCGCTGCCGG ATGGTGTTCC GCACCCAGCT GACCCGTGAC 
GATGGCACCA CCGAGACCCT CCAGGTCTGC TCGAATCCAA TCATCTGCAC TCAACCACCT 
GGCGTGCCGG AAATATGCAA GAAATCCTTA AACTCATGTC CCGTTGATGG CGGCCTCGAG 
CTTTTCATTA TCGGCAAGAA CTTTCTGAAG GACACACACG TGGTGTTTCA GGAGACATAC 
GACAGCGTCA ACGGTGACGA TCCGGCCACG GAAATTGCAG TGCGCCAGCA GCTCATCGGC 
GGTACAGCCG CTCTTTGGGA ACAGAGTGTT CTGCCGGATA AGGAGTACCT TCACCAGACT 
CATCTGATAT GCACGGTGCC ACCGTATCTG CACCAGAACA TCCTGAAACC GGTCCAGGTT 
CAAGTGTCGA TCGTCTCCAG CGGCAAGAAG AGCGAACCGC ATACGTTCAC CTACACGGCC 
AAGGGACAGT ACACGACGCT GGCGGCGGCC AGCACGTTAA GTAACACAAT CCACGCCCAA 
GCAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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NFAT cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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