wapl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wapl
Entrez Gene ID	31187
Full Name	wings apart-like
Synonyms	C204,CG3707,Dmel\CG3707,EG:87B1.2,N2,RNA D,RNAD,Wapl,l(1)2Dd,l(1)2Df,l(1)C204,l(1)N2,l(1)N5[72],l74,pasc,wap
General protein information	Preferred Names wings apart-like Names CG3707 gene product from transcript CG3707-RC CG3707-PA CG3707-PB CG3707-PC parallel sister chromatids protein wapl-PA wapl-PB wapl-PC wings-apart-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2D4-2D5\|1-0.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297875.1	NP_001284804.1	wings apart-like, isoform C
NM_080303.3	NP_525042.2	wings apart-like, isoform A
NM_166931.2	NP_726796.2	wings apart-like, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings suggests that the mutant Wapl-AG might exert its effects through changes in cohesin binding; Wapl-AG was found to increase the stability of cohesin binding to polytene chromosomes
Title: Wapl antagonizes cohesin binding and promotes Polycomb-group silencing in Drosophila.

Study shows that DNA replication and cohesin acetylation promote binding of Sororin to cohesin, and that Sororin displaces Wapl from its binding partner Pds5; in the absence of Wapl, Sororin becomes dispensable for cohesion.
Title: Sororin mediates sister chromatid cohesion by antagonizing Wapl.

Nipped-B, Pds5, and the Wapl protein that interacts with Pds5 all play unique roles in cohesin chromosome binding.
Title: Dosage-sensitive regulation of cohesin chromosome binding and dynamics by Nipped-B, Pds5, and Wapl.

The following wapl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wapl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00564	NM_166931.2 Latest version!	Drosophila melanogaster wings apart-like (wapl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00563	NM_080303.3 Latest version!	Drosophila melanogaster wings apart-like (wapl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa00563	NM_001297875.1 Latest version!	Drosophila melanogaster wings apart-like (wapl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wapl ( NM_166931.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726796.2 Drosophila melanogaster wings apart-like (wapl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00564
Clone ID Related Accession (Same CDS sequence)	NM_166931.2
Accession Version	NM_166931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wings apart-like, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166931.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGATGA CCATCGACGG AGCGGGAACG GGAGCAGCAT CTGCAGTGCC GGAGACACCA 
CTGCAGGAGG ACATACTGGC TGTGCTGCGA GGGGAAGTGC CACGGCTAAA TGGCAATACG 
GACCCGGAGC CAACCGAGGA GGAGGATCAA CAGCAACAGC CGAAGAGGGC CACGCGTGGC 
AGGGGCAGAA AGGCCAATAA CAATGTGGAT GTAACCCCAC CCGCCACGGA GACCAGAACC 
CGAGGTAGGG CCAAAGGAGC AGATGCGACC ACGGCTGCCA TATCGCCACC AACGGGCAAA 
AGAAACACGC GGGGCACCCG GGGCTCCAGA AAGGCCGAGC AGGAAGTCGA CATGGAAGTG 
GACGAAACGG CGATGACGAC GGTGCCAGCG AACGAGGAAC AGCTGGAACA GGCCACACTT 
CCACCGAGGA GAGGTCGCAA TGCTGCTGCC CGAGCCAATA ACAATAATTT GGCAAGCGTT 
AACAATAACA TTAATAAAAT AGCCGCCAAT TTGTCAGCCA AGGCCGAGGC CAGCCGACTA 
GCAGAAGGCG GAGTCGCGGG TGGAGCGGCA CGAAGCTATG GTCGGAAACG AAAGAACCAG 
CAGGTAACGC AGGTGCTACA GCAGGAGCCA GTGCCCGAAG AGCAGGAAAC TCCTGATGCT 
GAGGAGGAGC AGCCCACACC CGCCAAGATT CCGCACACAG ATCACAGGGA ACATTCGCCA 
GACCATGATC CGGATCCTGA TCCGGATGAA CTGTCGAACA ACTCAAACAA CTCCTCGTTG 
CAGCACGATG GGTCCTCCTC CTCGCCCCCA CCCCGCGATT TCAAGTTTAA GGATAAGTTT 
AAGCGAACAT TGACACTGGA CACACAGGGC GCGGCGAACG CCGGAGCGGG AGGAGCAGCA 
GCGGCGGCGC CACCTGAGTC CTCTGGCGAA CAGCGGGGCG CTGTCAAGCT GGTCATCTCA 
AAGAAGAAAG GCAGCATCTT CAAAAGCCGC GCTCTGGTGC CATCCGATCA AGCGGAACAG 
GCTACAGTGG CCAAGCGACA TCTGTACAAG CACAGTTGGG ATGCTGCGCT AGAAGCGAAT 
GGCGGTGGAA CCAACAGCGA TGCCAGCAAT GCCTCGGCAT CTGGCGTGGG CGTCGCTGGG 
GCGAAGGATC ATCTGCATCA TTTAGCGGCG GGCAAGTCCG ATGGTGATTT CGGTGACAGT 
CCGTCGTCGA ACAACAATGG CTCCTCCAGT GCGTGCAGCA GCGCATCCAC GTTGCGCGGC 
GATAGCCCGG CCCTCGGAAA GATCTCGCGA CTGGCGGGAA AACAGGGAGT ACCTGCCACC 
TCCACTAGTT CCGATGCCTT TGACCTGGAT TTGGAACCAA TTGCCGGAGA GCTTGACCTA 
GAGCGTAGTG CAGCTGGTGC TTCCGCTGGT GGAACAGGGG CAACGACAGG CGGAGGTGGA 
GCGACGGGCG GTGGCGGCCC CGTTCGGGTC GACCGCAAAA CTAAGGACTA CTATCCAGTG 
GTGCGTAACG TTAAGACGGC CCATCAAATT CAGGAGATCG GCGAGTACCA GGAAATGGAC 
GACGACGTCG AGTACATCCT GGACGCACTT CAGCCGCACA ATCCCCCGGC AACACGGTGT 
CTCTCCGCCC TTCAGCTAGC CGCCAAGTGT ATGATGCCCG CCTTCCGGAT GCACGTGCGT 
GCCCATGGGG TGGTCACCAA ATTTTTCAAG GCATTATCCG ACGCCAACAA GGACCTCAGC 
CTAGGCCTGT GCACCTCGGC CATCATGTAC ATTCTTTCCC AGGAGGGCCT CAACATGGAC 
CTGGATCGCG ACTCCTTGGA GCTGATGATC AACCTTCTGG AGGCGGACGG CGTGGGTGGC 
AGCACGGAGA CTGGGCACCC GGATAGAGCG GGCTACGACC GCAACAAGCA GAAGGTGCGC 
GAGTTGTGCG AGGAGATCAA GGCGCAGGGC AAGGGAACGC ATCTCAACGT TGATTCTCTG 
ACTGTGGGCA CGCTGGCAAT GGAAACGCTG CTATCGCTAA CATCCAAGCG CGCGGGCGAG 
TGGTTTAAAG AGGATCTGCG CAAGCTGGGT GGCCTGGAGC ACATTATCAA GACCATCTCG 
GACTTCTGCA GACCGGTGAT TGCCTGCGAC ACGGAGATTG ACTGGCAGCC GACGCTGCTG 
GATAACATGC AAACGGTGGC GCGTTGTCTG CGAGTCCTCG AAAACGTGAC GCAGCACAAT 
GAGACGAACC AGCGCTACAT GCTCACCTCT GGCCAGGGAA AAGCAGTGGA GACGCTTTGC 
CAACTGTACC GTCTTTGCAG CCGACAAATA ATGCTGCATC CTTCGGATGG TGGTGGCAGC 
AACAAGGAGC ATCCGGGTGT GGCTATGCGC GAGCTGCTGG TGCCGGTGCT CAAGGTACTG 
ATCAACCTGA CGCACACGTT CAACGAGGCG CAGCCATCGT TGGGAGCCGA GCTGCTAGGT 
CAAAGGGGCG ATGTGGTGGA GACGAGCTTC CGATTGCTGC TGCTCTCGGC CAACTACATT 
CCCGACCAAT GTGTCTTTGA GCTAAGCATA CTGGTTCTTA CACTGCTAAT CAATTTGTGC 
ATGCATACTG TGCCCAACCG GGCTGCTCTA ATGCAAGCTG CCGCTCCGGC AGAGTACGTA 
GCGGATAATC CACCAGCGCA GGGATCTGTG AGTGCACTGC AAGCTCTGCT TGAGTACTTC 
TACAAGTGCG AGGAGCTGGC TAGATTGGTG GAAAAGAACA CGGACGCCTT CCTCGAGAGC 
AACGAGAAGG GAAAGAAGAA ACAAGAAGAA GTGGAGGAGA CAGTCAACAA TCTTGTGCAA 
CGAGCCGGCC ACCACATGGA GCACACGCTA AAGGGAAGTT ATGCGGCCAT CCTGGTGGGA 
AATCTGATAG CGGACAACGA GTTGTACGAG TCGGTGGTGC GCCGCCAGCT GCGAGGAAAT 
AGCTTTAAGG AGATTATTGG AGTGCTGGAG AAGTACCACA CATTCATGAA CCTTACATCC 
AGCTTGGAGG CAGCCTTTGT GGCGCATATG AAGTCCACGA AGCGCATCAT CGACAACTTT 
AAGAAGCGCG ACTACATCTA CGAGCACTCG GATGAGCACG ACAACCCCCT GCCTCTGAAT 
CTGGAAACGA CGGCGCAAGT CTTGGCCGTG GGAGCGGACG CGTCGCATGC TGCTACAAGC 
TCGACCACGG TCGGCTCTGG CTCCGCACCC TCATCCACAT CGGCCACAGG AACGACGAGG 
GCGCCGCGGG TCTATAAAAC GTACAGCAGC CACAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726796.2
CDS	1019..4297
Translation	MMMTIDGAGTGAASAVPETPLQEDILAVLRGEVPRLNGNTDPEPTEEEDQQQQPKRATRGRGRKANNNVDVTPPATETRTRGRAKGADATTAAISPPTGKRNTRGTRGSRKAEQEVDMEVDETAMTTVPANEEQLEQATLPPRRGRNAAARANNNNLASVNNNINKIAANLSAKAEASRLAEGGVAGGAARSYGRKRKNQQVTQVLQQEPVPEEQETPDAEEEQPTPAKIPHTDHREHSPDHDPDPDPDELSNNSNNSSLQHDGSSSSPPPRDFKFKDKFKRTLTLDTQGAANAGAGGAAAAAPPESSGEQRGAVKLVISKKKGSIFKSRALVPSDQAEQATVAKRHLYKHSWDAALEANGGGTNSDASNASASGVGVAGAKDHLHHLAAGKSDGDFGDSPSSNNNGSSSACSSASTLRGDSPALGKISRLAGKQGVPATSTSSDAFDLDLEPIAGELDLERSAAGASAGGTGATTGGGGATGGGGPVRVDRKTKDYYPVVRNVKTAHQIQEIGEYQEMDDDVEYILDALQPHNPPATRCLSALQLAAKCMMPAFRMHVRAHGVVTKFFKALSDANKDLSLGLCTSAIMYILSQEGLNMDLDRDSLELMINLLEADGVGGSTETGHPDRAGYDRNKQKVRELCEEIKAQGKGTHLNVDSLTVGTLAMETLLSLTSKRAGEWFKEDLRKLGGLEHIIKTISDFCRPVIACDTEIDWQPTLLDNMQTVARCLRVLENVTQHNETNQRYMLTSGQGKAVETLCQLYRLCSRQIMLHPSDGGGSNKEHPGVAMRELLVPVLKVLINLTHTFNEAQPSLGAELLGQRGDVVETSFRLLLLSANYIPDQCVFELSILVLTLLINLCMHTVPNRAALMQAAAPAEYVADNPPAQGSVSALQALLEYFYKCEELARLVEKNTDAFLESNEKGKKKQEEVEETVNNLVQRAGHHMEHTLKGSYAAILVGNLIADNELYESVVRRQLRGNSFKEIIGVLEKYHTFMNLTSSLEAAFVAHMKSTKRIIDNFKKRDYIYEHSDEHDNPLPLNLETTAQVLAVGADASHAATSSTTVGSGSAPSSTSATGTTRAPRVYKTYSSHR

Target ORF information:

RefSeq Version	NM_166931.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wings apart-like (wapl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166931.2

ORF Insert Sequence:

ATGATGATGA CCATCGACGG AGCGGGAACG GGAGCAGCAT CTGCAGTGCC GGAGACACCA 
CTGCAGGAGG ACATACTGGC TGTGCTGCGA GGGGAAGTGC CACGGCTAAA TGGCAATACG 
GACCCGGAGC CAACCGAGGA GGAGGATCAA CAGCAACAGC CGAAGAGGGC CACGCGTGGC 
AGGGGCAGAA AGGCCAATAA CAATGTGGAT GTAACCCCAC CCGCCACGGA GACCAGAACC 
CGAGGTAGGG CCAAAGGAGC AGATGCGACC ACGGCTGCCA TATCGCCACC AACGGGCAAA 
AGAAACACGC GGGGCACCCG GGGCTCCAGA AAGGCCGAGC AGGAAGTCGA CATGGAAGTG 
GACGAAACGG CGATGACGAC GGTGCCAGCG AACGAGGAAC AGCTGGAACA GGCCACACTT 
CCACCGAGGA GAGGTCGCAA TGCTGCTGCC CGAGCCAATA ACAATAATTT GGCAAGCGTT 
AACAATAACA TTAATAAAAT AGCCGCCAAT TTGTCAGCCA AGGCCGAGGC CAGCCGACTA 
GCAGAAGGCG GAGTCGCGGG TGGAGCGGCA CGAAGCTATG GTCGGAAACG AAAGAACCAG 
CAGGTAACGC AGGTGCTACA GCAGGAGCCA GTGCCCGAAG AGCAGGAAAC TCCTGATGCT 
GAGGAGGAGC AGCCCACACC CGCCAAGATT CCGCACACAG ATCACAGGGA ACATTCGCCA 
GACCATGATC CGGATCCTGA TCCGGATGAA CTGTCGAACA ACTCAAACAA CTCCTCGTTG 
CAGCACGATG GGTCCTCCTC CTCGCCCCCA CCCCGCGATT TCAAGTTTAA GGATAAGTTT 
AAGCGAACAT TGACACTGGA CACACAGGGC GCGGCGAACG CCGGAGCGGG AGGAGCAGCA 
GCGGCGGCGC CACCTGAGTC CTCTGGCGAA CAGCGGGGCG CTGTCAAGCT GGTCATCTCA 
AAGAAGAAAG GCAGCATCTT CAAAAGCCGC GCTCTGGTGC CATCCGATCA AGCGGAACAG 
GCTACAGTGG CCAAGCGACA TCTGTACAAG CACAGTTGGG ATGCTGCGCT AGAAGCGAAT 
GGCGGTGGAA CCAACAGCGA TGCCAGCAAT GCCTCGGCAT CTGGCGTGGG CGTCGCTGGG 
GCGAAGGATC ATCTGCATCA TTTAGCGGCG GGCAAGTCCG ATGGTGATTT CGGTGACAGT 
CCGTCGTCGA ACAACAATGG CTCCTCCAGT GCGTGCAGCA GCGCATCCAC GTTGCGCGGC 
GATAGCCCGG CCCTCGGAAA GATCTCGCGA CTGGCGGGAA AACAGGGAGT ACCTGCCACC 
TCCACTAGTT CCGATGCCTT TGACCTGGAT TTGGAACCAA TTGCCGGAGA GCTTGACCTA 
GAGCGTAGTG CAGCTGGTGC TTCCGCTGGT GGAACAGGGG CAACGACAGG CGGAGGTGGA 
GCGACGGGCG GTGGCGGCCC CGTTCGGGTC GACCGCAAAA CTAAGGACTA CTATCCAGTG 
GTGCGTAACG TTAAGACGGC CCATCAAATT CAGGAGATCG GCGAGTACCA GGAAATGGAC 
GACGACGTCG AGTACATCCT GGACGCACTT CAGCCGCACA ATCCCCCGGC AACACGGTGT 
CTCTCCGCCC TTCAGCTAGC CGCCAAGTGT ATGATGCCCG CCTTCCGGAT GCACGTGCGT 
GCCCATGGGG TGGTCACCAA ATTTTTCAAG GCATTATCCG ACGCCAACAA GGACCTCAGC 
CTAGGCCTGT GCACCTCGGC CATCATGTAC ATTCTTTCCC AGGAGGGCCT CAACATGGAC 
CTGGATCGCG ACTCCTTGGA GCTGATGATC AACCTTCTGG AGGCGGACGG CGTGGGTGGC 
AGCACGGAGA CTGGGCACCC GGATAGAGCG GGCTACGACC GCAACAAGCA GAAGGTGCGC 
GAGTTGTGCG AGGAGATCAA GGCGCAGGGC AAGGGAACGC ATCTCAACGT TGATTCTCTG 
ACTGTGGGCA CGCTGGCAAT GGAAACGCTG CTATCGCTAA CATCCAAGCG CGCGGGCGAG 
TGGTTTAAAG AGGATCTGCG CAAGCTGGGT GGCCTGGAGC ACATTATCAA GACCATCTCG 
GACTTCTGCA GACCGGTGAT TGCCTGCGAC ACGGAGATTG ACTGGCAGCC GACGCTGCTG 
GATAACATGC AAACGGTGGC GCGTTGTCTG CGAGTCCTCG AAAACGTGAC GCAGCACAAT 
GAGACGAACC AGCGCTACAT GCTCACCTCT GGCCAGGGAA AAGCAGTGGA GACGCTTTGC 
CAACTGTACC GTCTTTGCAG CCGACAAATA ATGCTGCATC CTTCGGATGG TGGTGGCAGC 
AACAAGGAGC ATCCGGGTGT GGCTATGCGC GAGCTGCTGG TGCCGGTGCT CAAGGTACTG 
ATCAACCTGA CGCACACGTT CAACGAGGCG CAGCCATCGT TGGGAGCCGA GCTGCTAGGT 
CAAAGGGGCG ATGTGGTGGA GACGAGCTTC CGATTGCTGC TGCTCTCGGC CAACTACATT 
CCCGACCAAT GTGTCTTTGA GCTAAGCATA CTGGTTCTTA CACTGCTAAT CAATTTGTGC 
ATGCATACTG TGCCCAACCG GGCTGCTCTA ATGCAAGCTG CCGCTCCGGC AGAGTACGTA 
GCGGATAATC CACCAGCGCA GGGATCTGTG AGTGCACTGC AAGCTCTGCT TGAGTACTTC 
TACAAGTGCG AGGAGCTGGC TAGATTGGTG GAAAAGAACA CGGACGCCTT CCTCGAGAGC 
AACGAGAAGG GAAAGAAGAA ACAAGAAGAA GTGGAGGAGA CAGTCAACAA TCTTGTGCAA 
CGAGCCGGCC ACCACATGGA GCACACGCTA AAGGGAAGTT ATGCGGCCAT CCTGGTGGGA 
AATCTGATAG CGGACAACGA GTTGTACGAG TCGGTGGTGC GCCGCCAGCT GCGAGGAAAT 
AGCTTTAAGG AGATTATTGG AGTGCTGGAG AAGTACCACA CATTCATGAA CCTTACATCC 
AGCTTGGAGG CAGCCTTTGT GGCGCATATG AAGTCCACGA AGCGCATCAT CGACAACTTT 
AAGAAGCGCG ACTACATCTA CGAGCACTCG GATGAGCACG ACAACCCCCT GCCTCTGAAT 
CTGGAAACGA CGGCGCAAGT CTTGGCCGTG GGAGCGGACG CGTCGCATGC TGCTACAAGC 
TCGACCACGG TCGGCTCTGG CTCCGCACCC TCATCCACAT CGGCCACAGG AACGACGAGG 
GCGCCGCGGG TCTATAAAAC GTACAGCAGC CACAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wapl ( NM_001297875.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284804.1 Drosophila melanogaster wings apart-like (wapl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00563
Clone ID Related Accession (Same CDS sequence)	NM_001297875.1 , NM_080303.3
Accession Version	NM_001297875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wings apart-like, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCGCT GGGGCAAGAA CATCGTGGTG CCGCTGGACT CACTCTGCAA GGAGAAGGAG 
AACACCAACC GGCCCACTGT CGCCCGTTCC GTGGGCACCG TTGGCAAGTG GGGCAAGATG 
GGCTTCACCT CCACGCGCAC CTACACCCTG CCCGCCATCC ATCCTATGGC TGCGGCAGCG 
GCGGCTGCTG CAGCAGCCGC CAGTCCCTCC CAGAGTCCCG CCTCCACGCA GGATCACGAT 
CCCAACGACC TGTCCGTTTC GGTTCCGGAG CCGCCGAAGC CGAAAAAGTT CTTCAAATCG 
AGGAATACAG CTCCGCCGGA GGTCATAGCT CAGATCATTC AGCAGCTACC GCACTGTGGA 
GCCGGCGCAT CACCCATGCG AGACCATTTC TCGTCGGCGG GTGCGGGTGC TGGTGGTCTC 
ACGCCTACTT CCGGTGCCCA GGAGGCGGGC GGAGTGAAGC TGAAGCCGGG CAAGGGCGCC 
AGTTCCGCAG AGCGGAAGCG CAAATCGCCG AAAAAGAAGG CAGCAACAAC GTCAGCCTCC 
ACGCCCTCGA CGCCTGGTGC GTTCTACGGC GCCTCCGATC GAGATGGCGA TGGGCTAAGC 
GATCCCGCCT CAGAGCAGCC GGAGCAACCG TCCAGTGCCT CGGGCAAGCA GAAGCAGAAG 
AAGCCGAAGG AGGAGAAGAA GCTGAAGCCA GAGGCGCCGC CTTCGCGGGT ACTGGGACGC 
GCCCGCAAGG CTGTCAACTA CCGCGAAGTG GACGAGGACG AGCGCTATCC CACGCCCACC 
AAGGATCTGA TCATTCCCAA AGCGGGGCGC CAACCGGCTG AAGTGGCGGC TACGGCAACA 
CTTGCCGCCG CTTCGTCGGA AGCCTTCATC AGTTCCACGT TTGGCAGCCC TGGATCGGAG 
CCGTCGTTAC CCCCGCCTAC GTCAGCGCCA AGTGCATCTG CGTCCACCTC GTCCCAACTG 
CCCTCCGCAT CAGGCAGCGC GTCAAATCCT CCCAGCGCCT CCCGCACGCC AGAACATCCT 
CCTATCGTGC TACGCATCTC CAAGGGCACT TCGCGGTTGG TCAGCACGGA TAGCGAGGAG 
CCGCCGAGCA GCTCGCCCGC TCACCAGAAC CAACTGAATC AACTTTCGGT CACGGAGGAG 
GAGCCAGCGG AGCGGTCCGG AGACGAAACA GTTCCTGCAA GTACGCCAAA AATCACAGTG 
AAGCCACTGA GGCCGCCCAC AGCAGCAGAT TCCGTCGATG GATCATCTGC GGCAGTTGGA 
GGAGCATCAG CAGGCGATTC TTTCGAGGAA CGCAAGTCCC AGTCACTAGA ACCCAACGAG 
GACGAGGAGG AGGAAGAAGA GGAGGAGGAC GAGGAGGAGG AACCGCCGGA GATCAACTAC 
TGCACGGTAA AGATATCCCC GGACAAACCG CCGAAGGAGC GGCTCAAACT GATCATCAAG 
ACGGACGTGA TCCGCAACGC CATCGCCAAA GCCGCAGCAG CAGCCGAGTC CCGCAGTGAA 
AAGAAGTCGA GGAGCAAAAA GCACAAGCAC AAGCAGCTGC TTGCCGCGGG ATCTGGTGCC 
GCTCCAGCTT CTGGAGCCAC CCCCGCCGAG ATCAACTCCG AATTCAAGAC ACCCTCACCT 
CATTTGGCTC TCAGCGAGGC CAATAGTCAA CAAGCACAGC ATACACCATC TCATCTACAT 
CAACTGCACC AGCTGCATCC GCAGCGAGGC TCCGCGGTCA TATCACCAAC CACTCGATCC 
GATCACGACT TCGACTCGCA GTCCTCGGTG CTGGGCAGCA TCTCCTCGAA GGGCAACAGC 
ACGCCGCAAC TGTTAGCGCA GGCTGTGCAG GAGGATAGTT GTGTGATTCG CAGCAGGGGA 
TCTAGTGTGA TCACCAGTGA TCTAGAGACG AGTCAGCACT CCTCGCTGGT GGCTCCCCCC 
TCGGACATTG AGTCACGACT GGAGTCCATG ATGATGACCA TCGACGGAGC GGGAACGGGA 
GCAGCATCTG CAGTGCCGGA GACACCACTG CAGGAGGACA TACTGGCTGT GCTGCGAGGG 
GAAGTGCCAC GGCTAAATGG CAATACGGAC CCGGAGCCAA CCGAGGAGGA GGATCAACAG 
CAACAGCCGA AGAGGGCCAC GCGTGGCAGG GGCAGAAAGG CCAATAACAA TGTGGATGTA 
ACCCCACCCG CCACGGAGAC CAGAACCCGA GGTAGGGCCA AAGGAGCAGA TGCGACCACG 
GCTGCCATAT CGCCACCAAC GGGCAAAAGA AACACGCGGG GCACCCGGGG CTCCAGAAAG 
GCCGAGCAGG AAGTCGACAT GGAAGTGGAC GAAACGGCGA TGACGACGGT GCCAGCGAAC 
GAGGAACAGC TGGAACAGGC CACACTTCCA CCGAGGAGAG GTCGCAATGC TGCTGCCCGA 
GCCAATAACA ATAATTTGGC AAGCGTTAAC AATAACATTA ATAAAATAGC CGCCAATTTG 
TCAGCCAAGG CCGAGGCCAG CCGACTAGCA GAAGGCGGAG TCGCGGGTGG AGCGGCACGA 
AGCTATGGTC GGAAACGAAA GAACCAGCAG GTAACGCAGG TGCTACAGCA GGAGCCAGTG 
CCCGAAGAGC AGGAAACTCC TGATGCTGAG GAGGAGCAGC CCACACCCGC CAAGATTCCG 
CACACAGATC ACAGGGAACA TTCGCCAGAC CATGATCCGG ATCCTGATCC GGATGAACTG 
TCGAACAACT CAAACAACTC CTCGTTGCAG CACGATGGGT CCTCCTCCTC GCCCCCACCC 
CGCGATTTCA AGTTTAAGGA TAAGTTTAAG CGAACATTGA CACTGGACAC ACAGGGCGCG 
GCGAACGCCG GAGCGGGAGG AGCAGCAGCG GCGGCGCCAC CTGAGTCCTC TGGCGAACAG 
CGGGGCGCTG TCAAGCTGGT CATCTCAAAG AAGAAAGGCA GCATCTTCAA AAGCCGCGCT 
CTGGTGCCAT CCGATCAAGC GGAACAGGCT ACAGTGGCCA AGCGACATCT GTACAAGCAC 
AGTTGGGATG CTGCGCTAGA AGCGAATGGC GGTGGAACCA ACAGCGATGC CAGCAATGCC 
TCGGCATCTG GCGTGGGCGT CGCTGGGGCG AAGGATCATC TGCATCATTT AGCGGCGGGC 
AAGTCCGATG GTGATTTCGG TGACAGTCCG TCGTCGAACA ACAATGGCTC CTCCAGTGCG 
TGCAGCAGCG CATCCACGTT GCGCGGCGAT AGCCCGGCCC TCGGAAAGAT CTCGCGACTG 
GCGGGAAAAC AGGGAGTACC TGCCACCTCC ACTAGTTCCG ATGCCTTTGA CCTGGATTTG 
GAACCAATTG CCGGAGAGCT TGACCTAGAG CGTAGTGCAG CTGGTGCTTC CGCTGGTGGA 
ACAGGGGCAA CGACAGGCGG AGGTGGAGCG ACGGGCGGTG GCGGCCCCGT TCGGGTCGAC 
CGCAAAACTA AGGACTACTA TCCAGTGGTG CGTAACGTTA AGACGGCCCA TCAAATTCAG 
GAGATCGGCG AGTACCAGGA AATGGACGAC GACGTCGAGT ACATCCTGGA CGCACTTCAG 
CCGCACAATC CCCCGGCAAC ACGGTGTCTC TCCGCCCTTC AGCTAGCCGC CAAGTGTATG 
ATGCCCGCCT TCCGGATGCA CGTGCGTGCC CATGGGGTGG TCACCAAATT TTTCAAGGCA 
TTATCCGACG CCAACAAGGA CCTCAGCCTA GGCCTGTGCA CCTCGGCCAT CATGTACATT 
CTTTCCCAGG AGGGCCTCAA CATGGACCTG GATCGCGACT CCTTGGAGCT GATGATCAAC 
CTTCTGGAGG CGGACGGCGT GGGTGGCAGC ACGGAGACTG GGCACCCGGA TAGAGCGGGC 
TACGACCGCA ACAAGCAGAA GGTGCGCGAG TTGTGCGAGG AGATCAAGGC GCAGGGCAAG 
GGAACGCATC TCAACGTTGA TTCTCTGACT GTGGGCACGC TGGCAATGGA AACGCTGCTA 
TCGCTAACAT CCAAGCGCGC GGGCGAGTGG TTTAAAGAGG ATCTGCGCAA GCTGGGTGGC 
CTGGAGCACA TTATCAAGAC CATCTCGGAC TTCTGCAGAC CGGTGATTGC CTGCGACACG 
GAGATTGACT GGCAGCCGAC GCTGCTGGAT AACATGCAAA CGGTGGCGCG TTGTCTGCGA 
GTCCTCGAAA ACGTGACGCA GCACAATGAG ACGAACCAGC GCTACATGCT CACCTCTGGC 
CAGGGAAAAG CAGTGGAGAC GCTTTGCCAA CTGTACCGTC TTTGCAGCCG ACAAATAATG 
CTGCATCCTT CGGATGGTGG TGGCAGCAAC AAGGAGCATC CGGGTGTGGC TATGCGCGAG 
CTGCTGGTGC CGGTGCTCAA GGTACTGATC AACCTGACGC ACACGTTCAA CGAGGCGCAG 
CCATCGTTGG GAGCCGAGCT GCTAGGTCAA AGGGGCGATG TGGTGGAGAC GAGCTTCCGA 
TTGCTGCTGC TCTCGGCCAA CTACATTCCC GACCAATGTG TCTTTGAGCT AAGCATACTG 
GTTCTTACAC TGCTAATCAA TTTGTGCATG CATACTGTGC CCAACCGGGC TGCTCTAATG 
CAAGCTGCCG CTCCGGCAGA GTACGTAGCG GATAATCCAC CAGCGCAGGG ATCTGTGAGT 
GCACTGCAAG CTCTGCTTGA GTACTTCTAC AAGTGCGAGG AGCTGGCTAG ATTGGTGGAA 
AAGAACACGG ACGCCTTCCT CGAGAGCAAC GAGAAGGGAA AGAAGAAACA AGAAGAAGTG 
GAGGAGACAG TCAACAATCT TGTGCAACGA GCCGGCCACC ACATGGAGCA CACGCTAAAG 
GGAAGTTATG CGGCCATCCT GGTGGGAAAT CTGATAGCGG ACAACGAGTT GTACGAGTCG 
GTGGTGCGCC GCCAGCTGCG AGGAAATAGC TTTAAGGAGA TTATTGGAGT GCTGGAGAAG 
TACCACACAT TCATGAACCT TACATCCAGC TTGGAGGCAG CCTTTGTGGC GCATATGAAG 
TCCACGAAGC GCATCATCGA CAACTTTAAG AAGCGCGACT ACATCTACGA GCACTCGGAT 
GAGCACGACA ACCCCCTGCC TCTGAATCTG GAAACGACGG CGCAAGTCTT GGCCGTGGGA 
GCGGACGCGT CGCATGCTGC TACAAGCTCG ACCACGGTCG GCTCTGGCTC CGCACCCTCA 
TCCACATCGG CCACAGGAAC GACGAGGGCG CCGCGGGTCT ATAAAACGTA CAGCAGCCAC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284804.1
CDS	773..5998
Translation	MSRWGKNIVVPLDSLCKEKENTNRPTVARSVGTVGKWGKMGFTSTRTYTLPAIHPMAAAAAAAAAAASPSQSPASTQDHDPNDLSVSVPEPPKPKKFFKSRNTAPPEVIAQIIQQLPHCGAGASPMRDHFSSAGAGAGGLTPTSGAQEAGGVKLKPGKGASSAERKRKSPKKKAATTSASTPSTPGAFYGASDRDGDGLSDPASEQPEQPSSASGKQKQKKPKEEKKLKPEAPPSRVLGRARKAVNYREVDEDERYPTPTKDLIIPKAGRQPAEVAATATLAAASSEAFISSTFGSPGSEPSLPPPTSAPSASASTSSQLPSASGSASNPPSASRTPEHPPIVLRISKGTSRLVSTDSEEPPSSSPAHQNQLNQLSVTEEEPAERSGDETVPASTPKITVKPLRPPTAADSVDGSSAAVGGASAGDSFEERKSQSLEPNEDEEEEEEEEDEEEEPPEINYCTVKISPDKPPKERLKLIIKTDVIRNAIAKAAAAAESRSEKKSRSKKHKHKQLLAAGSGAAPASGATPAEINSEFKTPSPHLALSEANSQQAQHTPSHLHQLHQLHPQRGSAVISPTTRSDHDFDSQSSVLGSISSKGNSTPQLLAQAVQEDSCVIRSRGSSVITSDLETSQHSSLVAPPSDIESRLESMMMTIDGAGTGAASAVPETPLQEDILAVLRGEVPRLNGNTDPEPTEEEDQQQQPKRATRGRGRKANNNVDVTPPATETRTRGRAKGADATTAAISPPTGKRNTRGTRGSRKAEQEVDMEVDETAMTTVPANEEQLEQATLPPRRGRNAAARANNNNLASVNNNINKIAANLSAKAEASRLAEGGVAGGAARSYGRKRKNQQVTQVLQQEPVPEEQETPDAEEEQPTPAKIPHTDHREHSPDHDPDPDPDELSNNSNNSSLQHDGSSSSPPPRDFKFKDKFKRTLTLDTQGAANAGAGGAAAAAPPESSGEQRGAVKLVISKKKGSIFKSRALVPSDQAEQATVAKRHLYKHSWDAALEANGGGTNSDASNASASGVGVAGAKDHLHHLAAGKSDGDFGDSPSSNNNGSSSACSSASTLRGDSPALGKISRLAGKQGVPATSTSSDAFDLDLEPIAGELDLERSAAGASAGGTGATTGGGGATGGGGPVRVDRKTKDYYPVVRNVKTAHQIQEIGEYQEMDDDVEYILDALQPHNPPATRCLSALQLAAKCMMPAFRMHVRAHGVVTKFFKALSDANKDLSLGLCTSAIMYILSQEGLNMDLDRDSLELMINLLEADGVGGSTETGHPDRAGYDRNKQKVRELCEEIKAQGKGTHLNVDSLTVGTLAMETLLSLTSKRAGEWFKEDLRKLGGLEHIIKTISDFCRPVIACDTEIDWQPTLLDNMQTVARCLRVLENVTQHNETNQRYMLTSGQGKAVETLCQLYRLCSRQIMLHPSDGGGSNKEHPGVAMRELLVPVLKVLINLTHTFNEAQPSLGAELLGQRGDVVETSFRLLLLSANYIPDQCVFELSILVLTLLINLCMHTVPNRAALMQAAAPAEYVADNPPAQGSVSALQALLEYFYKCEELARLVEKNTDAFLESNEKGKKKQEEVEETVNNLVQRAGHHMEHTLKGSYAAILVGNLIADNELYESVVRRQLRGNSFKEIIGVLEKYHTFMNLTSSLEAAFVAHMKSTKRIIDNFKKRDYIYEHSDEHDNPLPLNLETTAQVLAVGADASHAATSSTTVGSGSAPSSTSATGTTRAPRVYKTYSSHR

Target ORF information:

RefSeq Version	NM_001297875.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wings apart-like (wapl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297875.1

ORF Insert Sequence:

ATGTCGCGCT GGGGCAAGAA CATCGTGGTG CCGCTGGACT CACTCTGCAA GGAGAAGGAG 
AACACCAACC GGCCCACTGT CGCCCGTTCC GTGGGCACCG TTGGCAAGTG GGGCAAGATG 
GGCTTCACCT CCACGCGCAC CTACACCCTG CCCGCCATCC ATCCTATGGC TGCGGCAGCG 
GCGGCTGCTG CAGCAGCCGC CAGTCCCTCC CAGAGTCCCG CCTCCACGCA GGATCACGAT 
CCCAACGACC TGTCCGTTTC GGTTCCGGAG CCGCCGAAGC CGAAAAAGTT CTTCAAATCG 
AGGAATACAG CTCCGCCGGA GGTCATAGCT CAGATCATTC AGCAGCTACC GCACTGTGGA 
GCCGGCGCAT CACCCATGCG AGACCATTTC TCGTCGGCGG GTGCGGGTGC TGGTGGTCTC 
ACGCCTACTT CCGGTGCCCA GGAGGCGGGC GGAGTGAAGC TGAAGCCGGG CAAGGGCGCC 
AGTTCCGCAG AGCGGAAGCG CAAATCGCCG AAAAAGAAGG CAGCAACAAC GTCAGCCTCC 
ACGCCCTCGA CGCCTGGTGC GTTCTACGGC GCCTCCGATC GAGATGGCGA TGGGCTAAGC 
GATCCCGCCT CAGAGCAGCC GGAGCAACCG TCCAGTGCCT CGGGCAAGCA GAAGCAGAAG 
AAGCCGAAGG AGGAGAAGAA GCTGAAGCCA GAGGCGCCGC CTTCGCGGGT ACTGGGACGC 
GCCCGCAAGG CTGTCAACTA CCGCGAAGTG GACGAGGACG AGCGCTATCC CACGCCCACC 
AAGGATCTGA TCATTCCCAA AGCGGGGCGC CAACCGGCTG AAGTGGCGGC TACGGCAACA 
CTTGCCGCCG CTTCGTCGGA AGCCTTCATC AGTTCCACGT TTGGCAGCCC TGGATCGGAG 
CCGTCGTTAC CCCCGCCTAC GTCAGCGCCA AGTGCATCTG CGTCCACCTC GTCCCAACTG 
CCCTCCGCAT CAGGCAGCGC GTCAAATCCT CCCAGCGCCT CCCGCACGCC AGAACATCCT 
CCTATCGTGC TACGCATCTC CAAGGGCACT TCGCGGTTGG TCAGCACGGA TAGCGAGGAG 
CCGCCGAGCA GCTCGCCCGC TCACCAGAAC CAACTGAATC AACTTTCGGT CACGGAGGAG 
GAGCCAGCGG AGCGGTCCGG AGACGAAACA GTTCCTGCAA GTACGCCAAA AATCACAGTG 
AAGCCACTGA GGCCGCCCAC AGCAGCAGAT TCCGTCGATG GATCATCTGC GGCAGTTGGA 
GGAGCATCAG CAGGCGATTC TTTCGAGGAA CGCAAGTCCC AGTCACTAGA ACCCAACGAG 
GACGAGGAGG AGGAAGAAGA GGAGGAGGAC GAGGAGGAGG AACCGCCGGA GATCAACTAC 
TGCACGGTAA AGATATCCCC GGACAAACCG CCGAAGGAGC GGCTCAAACT GATCATCAAG 
ACGGACGTGA TCCGCAACGC CATCGCCAAA GCCGCAGCAG CAGCCGAGTC CCGCAGTGAA 
AAGAAGTCGA GGAGCAAAAA GCACAAGCAC AAGCAGCTGC TTGCCGCGGG ATCTGGTGCC 
GCTCCAGCTT CTGGAGCCAC CCCCGCCGAG ATCAACTCCG AATTCAAGAC ACCCTCACCT 
CATTTGGCTC TCAGCGAGGC CAATAGTCAA CAAGCACAGC ATACACCATC TCATCTACAT 
CAACTGCACC AGCTGCATCC GCAGCGAGGC TCCGCGGTCA TATCACCAAC CACTCGATCC 
GATCACGACT TCGACTCGCA GTCCTCGGTG CTGGGCAGCA TCTCCTCGAA GGGCAACAGC 
ACGCCGCAAC TGTTAGCGCA GGCTGTGCAG GAGGATAGTT GTGTGATTCG CAGCAGGGGA 
TCTAGTGTGA TCACCAGTGA TCTAGAGACG AGTCAGCACT CCTCGCTGGT GGCTCCCCCC 
TCGGACATTG AGTCACGACT GGAGTCCATG ATGATGACCA TCGACGGAGC GGGAACGGGA 
GCAGCATCTG CAGTGCCGGA GACACCACTG CAGGAGGACA TACTGGCTGT GCTGCGAGGG 
GAAGTGCCAC GGCTAAATGG CAATACGGAC CCGGAGCCAA CCGAGGAGGA GGATCAACAG 
CAACAGCCGA AGAGGGCCAC GCGTGGCAGG GGCAGAAAGG CCAATAACAA TGTGGATGTA 
ACCCCACCCG CCACGGAGAC CAGAACCCGA GGTAGGGCCA AAGGAGCAGA TGCGACCACG 
GCTGCCATAT CGCCACCAAC GGGCAAAAGA AACACGCGGG GCACCCGGGG CTCCAGAAAG 
GCCGAGCAGG AAGTCGACAT GGAAGTGGAC GAAACGGCGA TGACGACGGT GCCAGCGAAC 
GAGGAACAGC TGGAACAGGC CACACTTCCA CCGAGGAGAG GTCGCAATGC TGCTGCCCGA 
GCCAATAACA ATAATTTGGC AAGCGTTAAC AATAACATTA ATAAAATAGC CGCCAATTTG 
TCAGCCAAGG CCGAGGCCAG CCGACTAGCA GAAGGCGGAG TCGCGGGTGG AGCGGCACGA 
AGCTATGGTC GGAAACGAAA GAACCAGCAG GTAACGCAGG TGCTACAGCA GGAGCCAGTG 
CCCGAAGAGC AGGAAACTCC TGATGCTGAG GAGGAGCAGC CCACACCCGC CAAGATTCCG 
CACACAGATC ACAGGGAACA TTCGCCAGAC CATGATCCGG ATCCTGATCC GGATGAACTG 
TCGAACAACT CAAACAACTC CTCGTTGCAG CACGATGGGT CCTCCTCCTC GCCCCCACCC 
CGCGATTTCA AGTTTAAGGA TAAGTTTAAG CGAACATTGA CACTGGACAC ACAGGGCGCG 
GCGAACGCCG GAGCGGGAGG AGCAGCAGCG GCGGCGCCAC CTGAGTCCTC TGGCGAACAG 
CGGGGCGCTG TCAAGCTGGT CATCTCAAAG AAGAAAGGCA GCATCTTCAA AAGCCGCGCT 
CTGGTGCCAT CCGATCAAGC GGAACAGGCT ACAGTGGCCA AGCGACATCT GTACAAGCAC 
AGTTGGGATG CTGCGCTAGA AGCGAATGGC GGTGGAACCA ACAGCGATGC CAGCAATGCC 
TCGGCATCTG GCGTGGGCGT CGCTGGGGCG AAGGATCATC TGCATCATTT AGCGGCGGGC 
AAGTCCGATG GTGATTTCGG TGACAGTCCG TCGTCGAACA ACAATGGCTC CTCCAGTGCG 
TGCAGCAGCG CATCCACGTT GCGCGGCGAT AGCCCGGCCC TCGGAAAGAT CTCGCGACTG 
GCGGGAAAAC AGGGAGTACC TGCCACCTCC ACTAGTTCCG ATGCCTTTGA CCTGGATTTG 
GAACCAATTG CCGGAGAGCT TGACCTAGAG CGTAGTGCAG CTGGTGCTTC CGCTGGTGGA 
ACAGGGGCAA CGACAGGCGG AGGTGGAGCG ACGGGCGGTG GCGGCCCCGT TCGGGTCGAC 
CGCAAAACTA AGGACTACTA TCCAGTGGTG CGTAACGTTA AGACGGCCCA TCAAATTCAG 
GAGATCGGCG AGTACCAGGA AATGGACGAC GACGTCGAGT ACATCCTGGA CGCACTTCAG 
CCGCACAATC CCCCGGCAAC ACGGTGTCTC TCCGCCCTTC AGCTAGCCGC CAAGTGTATG 
ATGCCCGCCT TCCGGATGCA CGTGCGTGCC CATGGGGTGG TCACCAAATT TTTCAAGGCA 
TTATCCGACG CCAACAAGGA CCTCAGCCTA GGCCTGTGCA CCTCGGCCAT CATGTACATT 
CTTTCCCAGG AGGGCCTCAA CATGGACCTG GATCGCGACT CCTTGGAGCT GATGATCAAC 
CTTCTGGAGG CGGACGGCGT GGGTGGCAGC ACGGAGACTG GGCACCCGGA TAGAGCGGGC 
TACGACCGCA ACAAGCAGAA GGTGCGCGAG TTGTGCGAGG AGATCAAGGC GCAGGGCAAG 
GGAACGCATC TCAACGTTGA TTCTCTGACT GTGGGCACGC TGGCAATGGA AACGCTGCTA 
TCGCTAACAT CCAAGCGCGC GGGCGAGTGG TTTAAAGAGG ATCTGCGCAA GCTGGGTGGC 
CTGGAGCACA TTATCAAGAC CATCTCGGAC TTCTGCAGAC CGGTGATTGC CTGCGACACG 
GAGATTGACT GGCAGCCGAC GCTGCTGGAT AACATGCAAA CGGTGGCGCG TTGTCTGCGA 
GTCCTCGAAA ACGTGACGCA GCACAATGAG ACGAACCAGC GCTACATGCT CACCTCTGGC 
CAGGGAAAAG CAGTGGAGAC GCTTTGCCAA CTGTACCGTC TTTGCAGCCG ACAAATAATG 
CTGCATCCTT CGGATGGTGG TGGCAGCAAC AAGGAGCATC CGGGTGTGGC TATGCGCGAG 
CTGCTGGTGC CGGTGCTCAA GGTACTGATC AACCTGACGC ACACGTTCAA CGAGGCGCAG 
CCATCGTTGG GAGCCGAGCT GCTAGGTCAA AGGGGCGATG TGGTGGAGAC GAGCTTCCGA 
TTGCTGCTGC TCTCGGCCAA CTACATTCCC GACCAATGTG TCTTTGAGCT AAGCATACTG 
GTTCTTACAC TGCTAATCAA TTTGTGCATG CATACTGTGC CCAACCGGGC TGCTCTAATG 
CAAGCTGCCG CTCCGGCAGA GTACGTAGCG GATAATCCAC CAGCGCAGGG ATCTGTGAGT 
GCACTGCAAG CTCTGCTTGA GTACTTCTAC AAGTGCGAGG AGCTGGCTAG ATTGGTGGAA 
AAGAACACGG ACGCCTTCCT CGAGAGCAAC GAGAAGGGAA AGAAGAAACA AGAAGAAGTG 
GAGGAGACAG TCAACAATCT TGTGCAACGA GCCGGCCACC ACATGGAGCA CACGCTAAAG 
GGAAGTTATG CGGCCATCCT GGTGGGAAAT CTGATAGCGG ACAACGAGTT GTACGAGTCG 
GTGGTGCGCC GCCAGCTGCG AGGAAATAGC TTTAAGGAGA TTATTGGAGT GCTGGAGAAG 
TACCACACAT TCATGAACCT TACATCCAGC TTGGAGGCAG CCTTTGTGGC GCATATGAAG 
TCCACGAAGC GCATCATCGA CAACTTTAAG AAGCGCGACT ACATCTACGA GCACTCGGAT 
GAGCACGACA ACCCCCTGCC TCTGAATCTG GAAACGACGG CGCAAGTCTT GGCCGTGGGA 
GCGGACGCGT CGCATGCTGC TACAAGCTCG ACCACGGTCG GCTCTGGCTC CGCACCCTCA 
TCCACATCGG CCACAGGAAC GACGAGGGCG CCGCGGGTCT ATAAAACGTA CAGCAGCCAC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wapl ( NM_080303.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525042.2 Drosophila melanogaster wings apart-like (wapl), transcript variant A, mRNA.

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CloneID	OFa00563
Clone ID Related Accession (Same CDS sequence)	NM_001297875.1 , NM_080303.3
Accession Version	NM_080303.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wings apart-like, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080303.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCGCT GGGGCAAGAA CATCGTGGTG CCGCTGGACT CACTCTGCAA GGAGAAGGAG 
AACACCAACC GGCCCACTGT CGCCCGTTCC GTGGGCACCG TTGGCAAGTG GGGCAAGATG 
GGCTTCACCT CCACGCGCAC CTACACCCTG CCCGCCATCC ATCCTATGGC TGCGGCAGCG 
GCGGCTGCTG CAGCAGCCGC CAGTCCCTCC CAGAGTCCCG CCTCCACGCA GGATCACGAT 
CCCAACGACC TGTCCGTTTC GGTTCCGGAG CCGCCGAAGC CGAAAAAGTT CTTCAAATCG 
AGGAATACAG CTCCGCCGGA GGTCATAGCT CAGATCATTC AGCAGCTACC GCACTGTGGA 
GCCGGCGCAT CACCCATGCG AGACCATTTC TCGTCGGCGG GTGCGGGTGC TGGTGGTCTC 
ACGCCTACTT CCGGTGCCCA GGAGGCGGGC GGAGTGAAGC TGAAGCCGGG CAAGGGCGCC 
AGTTCCGCAG AGCGGAAGCG CAAATCGCCG AAAAAGAAGG CAGCAACAAC GTCAGCCTCC 
ACGCCCTCGA CGCCTGGTGC GTTCTACGGC GCCTCCGATC GAGATGGCGA TGGGCTAAGC 
GATCCCGCCT CAGAGCAGCC GGAGCAACCG TCCAGTGCCT CGGGCAAGCA GAAGCAGAAG 
AAGCCGAAGG AGGAGAAGAA GCTGAAGCCA GAGGCGCCGC CTTCGCGGGT ACTGGGACGC 
GCCCGCAAGG CTGTCAACTA CCGCGAAGTG GACGAGGACG AGCGCTATCC CACGCCCACC 
AAGGATCTGA TCATTCCCAA AGCGGGGCGC CAACCGGCTG AAGTGGCGGC TACGGCAACA 
CTTGCCGCCG CTTCGTCGGA AGCCTTCATC AGTTCCACGT TTGGCAGCCC TGGATCGGAG 
CCGTCGTTAC CCCCGCCTAC GTCAGCGCCA AGTGCATCTG CGTCCACCTC GTCCCAACTG 
CCCTCCGCAT CAGGCAGCGC GTCAAATCCT CCCAGCGCCT CCCGCACGCC AGAACATCCT 
CCTATCGTGC TACGCATCTC CAAGGGCACT TCGCGGTTGG TCAGCACGGA TAGCGAGGAG 
CCGCCGAGCA GCTCGCCCGC TCACCAGAAC CAACTGAATC AACTTTCGGT CACGGAGGAG 
GAGCCAGCGG AGCGGTCCGG AGACGAAACA GTTCCTGCAA GTACGCCAAA AATCACAGTG 
AAGCCACTGA GGCCGCCCAC AGCAGCAGAT TCCGTCGATG GATCATCTGC GGCAGTTGGA 
GGAGCATCAG CAGGCGATTC TTTCGAGGAA CGCAAGTCCC AGTCACTAGA ACCCAACGAG 
GACGAGGAGG AGGAAGAAGA GGAGGAGGAC GAGGAGGAGG AACCGCCGGA GATCAACTAC 
TGCACGGTAA AGATATCCCC GGACAAACCG CCGAAGGAGC GGCTCAAACT GATCATCAAG 
ACGGACGTGA TCCGCAACGC CATCGCCAAA GCCGCAGCAG CAGCCGAGTC CCGCAGTGAA 
AAGAAGTCGA GGAGCAAAAA GCACAAGCAC AAGCAGCTGC TTGCCGCGGG ATCTGGTGCC 
GCTCCAGCTT CTGGAGCCAC CCCCGCCGAG ATCAACTCCG AATTCAAGAC ACCCTCACCT 
CATTTGGCTC TCAGCGAGGC CAATAGTCAA CAAGCACAGC ATACACCATC TCATCTACAT 
CAACTGCACC AGCTGCATCC GCAGCGAGGC TCCGCGGTCA TATCACCAAC CACTCGATCC 
GATCACGACT TCGACTCGCA GTCCTCGGTG CTGGGCAGCA TCTCCTCGAA GGGCAACAGC 
ACGCCGCAAC TGTTAGCGCA GGCTGTGCAG GAGGATAGTT GTGTGATTCG CAGCAGGGGA 
TCTAGTGTGA TCACCAGTGA TCTAGAGACG AGTCAGCACT CCTCGCTGGT GGCTCCCCCC 
TCGGACATTG AGTCACGACT GGAGTCCATG ATGATGACCA TCGACGGAGC GGGAACGGGA 
GCAGCATCTG CAGTGCCGGA GACACCACTG CAGGAGGACA TACTGGCTGT GCTGCGAGGG 
GAAGTGCCAC GGCTAAATGG CAATACGGAC CCGGAGCCAA CCGAGGAGGA GGATCAACAG 
CAACAGCCGA AGAGGGCCAC GCGTGGCAGG GGCAGAAAGG CCAATAACAA TGTGGATGTA 
ACCCCACCCG CCACGGAGAC CAGAACCCGA GGTAGGGCCA AAGGAGCAGA TGCGACCACG 
GCTGCCATAT CGCCACCAAC GGGCAAAAGA AACACGCGGG GCACCCGGGG CTCCAGAAAG 
GCCGAGCAGG AAGTCGACAT GGAAGTGGAC GAAACGGCGA TGACGACGGT GCCAGCGAAC 
GAGGAACAGC TGGAACAGGC CACACTTCCA CCGAGGAGAG GTCGCAATGC TGCTGCCCGA 
GCCAATAACA ATAATTTGGC AAGCGTTAAC AATAACATTA ATAAAATAGC CGCCAATTTG 
TCAGCCAAGG CCGAGGCCAG CCGACTAGCA GAAGGCGGAG TCGCGGGTGG AGCGGCACGA 
AGCTATGGTC GGAAACGAAA GAACCAGCAG GTAACGCAGG TGCTACAGCA GGAGCCAGTG 
CCCGAAGAGC AGGAAACTCC TGATGCTGAG GAGGAGCAGC CCACACCCGC CAAGATTCCG 
CACACAGATC ACAGGGAACA TTCGCCAGAC CATGATCCGG ATCCTGATCC GGATGAACTG 
TCGAACAACT CAAACAACTC CTCGTTGCAG CACGATGGGT CCTCCTCCTC GCCCCCACCC 
CGCGATTTCA AGTTTAAGGA TAAGTTTAAG CGAACATTGA CACTGGACAC ACAGGGCGCG 
GCGAACGCCG GAGCGGGAGG AGCAGCAGCG GCGGCGCCAC CTGAGTCCTC TGGCGAACAG 
CGGGGCGCTG TCAAGCTGGT CATCTCAAAG AAGAAAGGCA GCATCTTCAA AAGCCGCGCT 
CTGGTGCCAT CCGATCAAGC GGAACAGGCT ACAGTGGCCA AGCGACATCT GTACAAGCAC 
AGTTGGGATG CTGCGCTAGA AGCGAATGGC GGTGGAACCA ACAGCGATGC CAGCAATGCC 
TCGGCATCTG GCGTGGGCGT CGCTGGGGCG AAGGATCATC TGCATCATTT AGCGGCGGGC 
AAGTCCGATG GTGATTTCGG TGACAGTCCG TCGTCGAACA ACAATGGCTC CTCCAGTGCG 
TGCAGCAGCG CATCCACGTT GCGCGGCGAT AGCCCGGCCC TCGGAAAGAT CTCGCGACTG 
GCGGGAAAAC AGGGAGTACC TGCCACCTCC ACTAGTTCCG ATGCCTTTGA CCTGGATTTG 
GAACCAATTG CCGGAGAGCT TGACCTAGAG CGTAGTGCAG CTGGTGCTTC CGCTGGTGGA 
ACAGGGGCAA CGACAGGCGG AGGTGGAGCG ACGGGCGGTG GCGGCCCCGT TCGGGTCGAC 
CGCAAAACTA AGGACTACTA TCCAGTGGTG CGTAACGTTA AGACGGCCCA TCAAATTCAG 
GAGATCGGCG AGTACCAGGA AATGGACGAC GACGTCGAGT ACATCCTGGA CGCACTTCAG 
CCGCACAATC CCCCGGCAAC ACGGTGTCTC TCCGCCCTTC AGCTAGCCGC CAAGTGTATG 
ATGCCCGCCT TCCGGATGCA CGTGCGTGCC CATGGGGTGG TCACCAAATT TTTCAAGGCA 
TTATCCGACG CCAACAAGGA CCTCAGCCTA GGCCTGTGCA CCTCGGCCAT CATGTACATT 
CTTTCCCAGG AGGGCCTCAA CATGGACCTG GATCGCGACT CCTTGGAGCT GATGATCAAC 
CTTCTGGAGG CGGACGGCGT GGGTGGCAGC ACGGAGACTG GGCACCCGGA TAGAGCGGGC 
TACGACCGCA ACAAGCAGAA GGTGCGCGAG TTGTGCGAGG AGATCAAGGC GCAGGGCAAG 
GGAACGCATC TCAACGTTGA TTCTCTGACT GTGGGCACGC TGGCAATGGA AACGCTGCTA 
TCGCTAACAT CCAAGCGCGC GGGCGAGTGG TTTAAAGAGG ATCTGCGCAA GCTGGGTGGC 
CTGGAGCACA TTATCAAGAC CATCTCGGAC TTCTGCAGAC CGGTGATTGC CTGCGACACG 
GAGATTGACT GGCAGCCGAC GCTGCTGGAT AACATGCAAA CGGTGGCGCG TTGTCTGCGA 
GTCCTCGAAA ACGTGACGCA GCACAATGAG ACGAACCAGC GCTACATGCT CACCTCTGGC 
CAGGGAAAAG CAGTGGAGAC GCTTTGCCAA CTGTACCGTC TTTGCAGCCG ACAAATAATG 
CTGCATCCTT CGGATGGTGG TGGCAGCAAC AAGGAGCATC CGGGTGTGGC TATGCGCGAG 
CTGCTGGTGC CGGTGCTCAA GGTACTGATC AACCTGACGC ACACGTTCAA CGAGGCGCAG 
CCATCGTTGG GAGCCGAGCT GCTAGGTCAA AGGGGCGATG TGGTGGAGAC GAGCTTCCGA 
TTGCTGCTGC TCTCGGCCAA CTACATTCCC GACCAATGTG TCTTTGAGCT AAGCATACTG 
GTTCTTACAC TGCTAATCAA TTTGTGCATG CATACTGTGC CCAACCGGGC TGCTCTAATG 
CAAGCTGCCG CTCCGGCAGA GTACGTAGCG GATAATCCAC CAGCGCAGGG ATCTGTGAGT 
GCACTGCAAG CTCTGCTTGA GTACTTCTAC AAGTGCGAGG AGCTGGCTAG ATTGGTGGAA 
AAGAACACGG ACGCCTTCCT CGAGAGCAAC GAGAAGGGAA AGAAGAAACA AGAAGAAGTG 
GAGGAGACAG TCAACAATCT TGTGCAACGA GCCGGCCACC ACATGGAGCA CACGCTAAAG 
GGAAGTTATG CGGCCATCCT GGTGGGAAAT CTGATAGCGG ACAACGAGTT GTACGAGTCG 
GTGGTGCGCC GCCAGCTGCG AGGAAATAGC TTTAAGGAGA TTATTGGAGT GCTGGAGAAG 
TACCACACAT TCATGAACCT TACATCCAGC TTGGAGGCAG CCTTTGTGGC GCATATGAAG 
TCCACGAAGC GCATCATCGA CAACTTTAAG AAGCGCGACT ACATCTACGA GCACTCGGAT 
GAGCACGACA ACCCCCTGCC TCTGAATCTG GAAACGACGG CGCAAGTCTT GGCCGTGGGA 
GCGGACGCGT CGCATGCTGC TACAAGCTCG ACCACGGTCG GCTCTGGCTC CGCACCCTCA 
TCCACATCGG CCACAGGAAC GACGAGGGCG CCGCGGGTCT ATAAAACGTA CAGCAGCCAC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525042.2
CDS	773..5998
Translation	MSRWGKNIVVPLDSLCKEKENTNRPTVARSVGTVGKWGKMGFTSTRTYTLPAIHPMAAAAAAAAAAASPSQSPASTQDHDPNDLSVSVPEPPKPKKFFKSRNTAPPEVIAQIIQQLPHCGAGASPMRDHFSSAGAGAGGLTPTSGAQEAGGVKLKPGKGASSAERKRKSPKKKAATTSASTPSTPGAFYGASDRDGDGLSDPASEQPEQPSSASGKQKQKKPKEEKKLKPEAPPSRVLGRARKAVNYREVDEDERYPTPTKDLIIPKAGRQPAEVAATATLAAASSEAFISSTFGSPGSEPSLPPPTSAPSASASTSSQLPSASGSASNPPSASRTPEHPPIVLRISKGTSRLVSTDSEEPPSSSPAHQNQLNQLSVTEEEPAERSGDETVPASTPKITVKPLRPPTAADSVDGSSAAVGGASAGDSFEERKSQSLEPNEDEEEEEEEEDEEEEPPEINYCTVKISPDKPPKERLKLIIKTDVIRNAIAKAAAAAESRSEKKSRSKKHKHKQLLAAGSGAAPASGATPAEINSEFKTPSPHLALSEANSQQAQHTPSHLHQLHQLHPQRGSAVISPTTRSDHDFDSQSSVLGSISSKGNSTPQLLAQAVQEDSCVIRSRGSSVITSDLETSQHSSLVAPPSDIESRLESMMMTIDGAGTGAASAVPETPLQEDILAVLRGEVPRLNGNTDPEPTEEEDQQQQPKRATRGRGRKANNNVDVTPPATETRTRGRAKGADATTAAISPPTGKRNTRGTRGSRKAEQEVDMEVDETAMTTVPANEEQLEQATLPPRRGRNAAARANNNNLASVNNNINKIAANLSAKAEASRLAEGGVAGGAARSYGRKRKNQQVTQVLQQEPVPEEQETPDAEEEQPTPAKIPHTDHREHSPDHDPDPDPDELSNNSNNSSLQHDGSSSSPPPRDFKFKDKFKRTLTLDTQGAANAGAGGAAAAAPPESSGEQRGAVKLVISKKKGSIFKSRALVPSDQAEQATVAKRHLYKHSWDAALEANGGGTNSDASNASASGVGVAGAKDHLHHLAAGKSDGDFGDSPSSNNNGSSSACSSASTLRGDSPALGKISRLAGKQGVPATSTSSDAFDLDLEPIAGELDLERSAAGASAGGTGATTGGGGATGGGGPVRVDRKTKDYYPVVRNVKTAHQIQEIGEYQEMDDDVEYILDALQPHNPPATRCLSALQLAAKCMMPAFRMHVRAHGVVTKFFKALSDANKDLSLGLCTSAIMYILSQEGLNMDLDRDSLELMINLLEADGVGGSTETGHPDRAGYDRNKQKVRELCEEIKAQGKGTHLNVDSLTVGTLAMETLLSLTSKRAGEWFKEDLRKLGGLEHIIKTISDFCRPVIACDTEIDWQPTLLDNMQTVARCLRVLENVTQHNETNQRYMLTSGQGKAVETLCQLYRLCSRQIMLHPSDGGGSNKEHPGVAMRELLVPVLKVLINLTHTFNEAQPSLGAELLGQRGDVVETSFRLLLLSANYIPDQCVFELSILVLTLLINLCMHTVPNRAALMQAAAPAEYVADNPPAQGSVSALQALLEYFYKCEELARLVEKNTDAFLESNEKGKKKQEEVEETVNNLVQRAGHHMEHTLKGSYAAILVGNLIADNELYESVVRRQLRGNSFKEIIGVLEKYHTFMNLTSSLEAAFVAHMKSTKRIIDNFKKRDYIYEHSDEHDNPLPLNLETTAQVLAVGADASHAATSSTTVGSGSAPSSTSATGTTRAPRVYKTYSSHR

Target ORF information:

RefSeq Version	NM_080303.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wings apart-like (wapl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080303.3

ORF Insert Sequence:

ATGTCGCGCT GGGGCAAGAA CATCGTGGTG CCGCTGGACT CACTCTGCAA GGAGAAGGAG 
AACACCAACC GGCCCACTGT CGCCCGTTCC GTGGGCACCG TTGGCAAGTG GGGCAAGATG 
GGCTTCACCT CCACGCGCAC CTACACCCTG CCCGCCATCC ATCCTATGGC TGCGGCAGCG 
GCGGCTGCTG CAGCAGCCGC CAGTCCCTCC CAGAGTCCCG CCTCCACGCA GGATCACGAT 
CCCAACGACC TGTCCGTTTC GGTTCCGGAG CCGCCGAAGC CGAAAAAGTT CTTCAAATCG 
AGGAATACAG CTCCGCCGGA GGTCATAGCT CAGATCATTC AGCAGCTACC GCACTGTGGA 
GCCGGCGCAT CACCCATGCG AGACCATTTC TCGTCGGCGG GTGCGGGTGC TGGTGGTCTC 
ACGCCTACTT CCGGTGCCCA GGAGGCGGGC GGAGTGAAGC TGAAGCCGGG CAAGGGCGCC 
AGTTCCGCAG AGCGGAAGCG CAAATCGCCG AAAAAGAAGG CAGCAACAAC GTCAGCCTCC 
ACGCCCTCGA CGCCTGGTGC GTTCTACGGC GCCTCCGATC GAGATGGCGA TGGGCTAAGC 
GATCCCGCCT CAGAGCAGCC GGAGCAACCG TCCAGTGCCT CGGGCAAGCA GAAGCAGAAG 
AAGCCGAAGG AGGAGAAGAA GCTGAAGCCA GAGGCGCCGC CTTCGCGGGT ACTGGGACGC 
GCCCGCAAGG CTGTCAACTA CCGCGAAGTG GACGAGGACG AGCGCTATCC CACGCCCACC 
AAGGATCTGA TCATTCCCAA AGCGGGGCGC CAACCGGCTG AAGTGGCGGC TACGGCAACA 
CTTGCCGCCG CTTCGTCGGA AGCCTTCATC AGTTCCACGT TTGGCAGCCC TGGATCGGAG 
CCGTCGTTAC CCCCGCCTAC GTCAGCGCCA AGTGCATCTG CGTCCACCTC GTCCCAACTG 
CCCTCCGCAT CAGGCAGCGC GTCAAATCCT CCCAGCGCCT CCCGCACGCC AGAACATCCT 
CCTATCGTGC TACGCATCTC CAAGGGCACT TCGCGGTTGG TCAGCACGGA TAGCGAGGAG 
CCGCCGAGCA GCTCGCCCGC TCACCAGAAC CAACTGAATC AACTTTCGGT CACGGAGGAG 
GAGCCAGCGG AGCGGTCCGG AGACGAAACA GTTCCTGCAA GTACGCCAAA AATCACAGTG 
AAGCCACTGA GGCCGCCCAC AGCAGCAGAT TCCGTCGATG GATCATCTGC GGCAGTTGGA 
GGAGCATCAG CAGGCGATTC TTTCGAGGAA CGCAAGTCCC AGTCACTAGA ACCCAACGAG 
GACGAGGAGG AGGAAGAAGA GGAGGAGGAC GAGGAGGAGG AACCGCCGGA GATCAACTAC 
TGCACGGTAA AGATATCCCC GGACAAACCG CCGAAGGAGC GGCTCAAACT GATCATCAAG 
ACGGACGTGA TCCGCAACGC CATCGCCAAA GCCGCAGCAG CAGCCGAGTC CCGCAGTGAA 
AAGAAGTCGA GGAGCAAAAA GCACAAGCAC AAGCAGCTGC TTGCCGCGGG ATCTGGTGCC 
GCTCCAGCTT CTGGAGCCAC CCCCGCCGAG ATCAACTCCG AATTCAAGAC ACCCTCACCT 
CATTTGGCTC TCAGCGAGGC CAATAGTCAA CAAGCACAGC ATACACCATC TCATCTACAT 
CAACTGCACC AGCTGCATCC GCAGCGAGGC TCCGCGGTCA TATCACCAAC CACTCGATCC 
GATCACGACT TCGACTCGCA GTCCTCGGTG CTGGGCAGCA TCTCCTCGAA GGGCAACAGC 
ACGCCGCAAC TGTTAGCGCA GGCTGTGCAG GAGGATAGTT GTGTGATTCG CAGCAGGGGA 
TCTAGTGTGA TCACCAGTGA TCTAGAGACG AGTCAGCACT CCTCGCTGGT GGCTCCCCCC 
TCGGACATTG AGTCACGACT GGAGTCCATG ATGATGACCA TCGACGGAGC GGGAACGGGA 
GCAGCATCTG CAGTGCCGGA GACACCACTG CAGGAGGACA TACTGGCTGT GCTGCGAGGG 
GAAGTGCCAC GGCTAAATGG CAATACGGAC CCGGAGCCAA CCGAGGAGGA GGATCAACAG 
CAACAGCCGA AGAGGGCCAC GCGTGGCAGG GGCAGAAAGG CCAATAACAA TGTGGATGTA 
ACCCCACCCG CCACGGAGAC CAGAACCCGA GGTAGGGCCA AAGGAGCAGA TGCGACCACG 
GCTGCCATAT CGCCACCAAC GGGCAAAAGA AACACGCGGG GCACCCGGGG CTCCAGAAAG 
GCCGAGCAGG AAGTCGACAT GGAAGTGGAC GAAACGGCGA TGACGACGGT GCCAGCGAAC 
GAGGAACAGC TGGAACAGGC CACACTTCCA CCGAGGAGAG GTCGCAATGC TGCTGCCCGA 
GCCAATAACA ATAATTTGGC AAGCGTTAAC AATAACATTA ATAAAATAGC CGCCAATTTG 
TCAGCCAAGG CCGAGGCCAG CCGACTAGCA GAAGGCGGAG TCGCGGGTGG AGCGGCACGA 
AGCTATGGTC GGAAACGAAA GAACCAGCAG GTAACGCAGG TGCTACAGCA GGAGCCAGTG 
CCCGAAGAGC AGGAAACTCC TGATGCTGAG GAGGAGCAGC CCACACCCGC CAAGATTCCG 
CACACAGATC ACAGGGAACA TTCGCCAGAC CATGATCCGG ATCCTGATCC GGATGAACTG 
TCGAACAACT CAAACAACTC CTCGTTGCAG CACGATGGGT CCTCCTCCTC GCCCCCACCC 
CGCGATTTCA AGTTTAAGGA TAAGTTTAAG CGAACATTGA CACTGGACAC ACAGGGCGCG 
GCGAACGCCG GAGCGGGAGG AGCAGCAGCG GCGGCGCCAC CTGAGTCCTC TGGCGAACAG 
CGGGGCGCTG TCAAGCTGGT CATCTCAAAG AAGAAAGGCA GCATCTTCAA AAGCCGCGCT 
CTGGTGCCAT CCGATCAAGC GGAACAGGCT ACAGTGGCCA AGCGACATCT GTACAAGCAC 
AGTTGGGATG CTGCGCTAGA AGCGAATGGC GGTGGAACCA ACAGCGATGC CAGCAATGCC 
TCGGCATCTG GCGTGGGCGT CGCTGGGGCG AAGGATCATC TGCATCATTT AGCGGCGGGC 
AAGTCCGATG GTGATTTCGG TGACAGTCCG TCGTCGAACA ACAATGGCTC CTCCAGTGCG 
TGCAGCAGCG CATCCACGTT GCGCGGCGAT AGCCCGGCCC TCGGAAAGAT CTCGCGACTG 
GCGGGAAAAC AGGGAGTACC TGCCACCTCC ACTAGTTCCG ATGCCTTTGA CCTGGATTTG 
GAACCAATTG CCGGAGAGCT TGACCTAGAG CGTAGTGCAG CTGGTGCTTC CGCTGGTGGA 
ACAGGGGCAA CGACAGGCGG AGGTGGAGCG ACGGGCGGTG GCGGCCCCGT TCGGGTCGAC 
CGCAAAACTA AGGACTACTA TCCAGTGGTG CGTAACGTTA AGACGGCCCA TCAAATTCAG 
GAGATCGGCG AGTACCAGGA AATGGACGAC GACGTCGAGT ACATCCTGGA CGCACTTCAG 
CCGCACAATC CCCCGGCAAC ACGGTGTCTC TCCGCCCTTC AGCTAGCCGC CAAGTGTATG 
ATGCCCGCCT TCCGGATGCA CGTGCGTGCC CATGGGGTGG TCACCAAATT TTTCAAGGCA 
TTATCCGACG CCAACAAGGA CCTCAGCCTA GGCCTGTGCA CCTCGGCCAT CATGTACATT 
CTTTCCCAGG AGGGCCTCAA CATGGACCTG GATCGCGACT CCTTGGAGCT GATGATCAAC 
CTTCTGGAGG CGGACGGCGT GGGTGGCAGC ACGGAGACTG GGCACCCGGA TAGAGCGGGC 
TACGACCGCA ACAAGCAGAA GGTGCGCGAG TTGTGCGAGG AGATCAAGGC GCAGGGCAAG 
GGAACGCATC TCAACGTTGA TTCTCTGACT GTGGGCACGC TGGCAATGGA AACGCTGCTA 
TCGCTAACAT CCAAGCGCGC GGGCGAGTGG TTTAAAGAGG ATCTGCGCAA GCTGGGTGGC 
CTGGAGCACA TTATCAAGAC CATCTCGGAC TTCTGCAGAC CGGTGATTGC CTGCGACACG 
GAGATTGACT GGCAGCCGAC GCTGCTGGAT AACATGCAAA CGGTGGCGCG TTGTCTGCGA 
GTCCTCGAAA ACGTGACGCA GCACAATGAG ACGAACCAGC GCTACATGCT CACCTCTGGC 
CAGGGAAAAG CAGTGGAGAC GCTTTGCCAA CTGTACCGTC TTTGCAGCCG ACAAATAATG 
CTGCATCCTT CGGATGGTGG TGGCAGCAAC AAGGAGCATC CGGGTGTGGC TATGCGCGAG 
CTGCTGGTGC CGGTGCTCAA GGTACTGATC AACCTGACGC ACACGTTCAA CGAGGCGCAG 
CCATCGTTGG GAGCCGAGCT GCTAGGTCAA AGGGGCGATG TGGTGGAGAC GAGCTTCCGA 
TTGCTGCTGC TCTCGGCCAA CTACATTCCC GACCAATGTG TCTTTGAGCT AAGCATACTG 
GTTCTTACAC TGCTAATCAA TTTGTGCATG CATACTGTGC CCAACCGGGC TGCTCTAATG 
CAAGCTGCCG CTCCGGCAGA GTACGTAGCG GATAATCCAC CAGCGCAGGG ATCTGTGAGT 
GCACTGCAAG CTCTGCTTGA GTACTTCTAC AAGTGCGAGG AGCTGGCTAG ATTGGTGGAA 
AAGAACACGG ACGCCTTCCT CGAGAGCAAC GAGAAGGGAA AGAAGAAACA AGAAGAAGTG 
GAGGAGACAG TCAACAATCT TGTGCAACGA GCCGGCCACC ACATGGAGCA CACGCTAAAG 
GGAAGTTATG CGGCCATCCT GGTGGGAAAT CTGATAGCGG ACAACGAGTT GTACGAGTCG 
GTGGTGCGCC GCCAGCTGCG AGGAAATAGC TTTAAGGAGA TTATTGGAGT GCTGGAGAAG 
TACCACACAT TCATGAACCT TACATCCAGC TTGGAGGCAG CCTTTGTGGC GCATATGAAG 
TCCACGAAGC GCATCATCGA CAACTTTAAG AAGCGCGACT ACATCTACGA GCACTCGGAT 
GAGCACGACA ACCCCCTGCC TCTGAATCTG GAAACGACGG CGCAAGTCTT GGCCGTGGGA 
GCGGACGCGT CGCATGCTGC TACAAGCTCG ACCACGGTCG GCTCTGGCTC CGCACCCTCA 
TCCACATCGG CCACAGGAAC GACGAGGGCG CCGCGGGTCT ATAAAACGTA CAGCAGCCAC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wapl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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