Hars cDNA ORF clone, Dipodomys ordii(Ord's kangaroo rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hars
Entrez Gene ID	105988735
Full Name	histidyl-tRNA synthetase
Gene Type	protein-coding
Organism	Dipodomys ordii(Ord's kangaroo rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013020444.1	XP_012875898.1	histidine--tRNA ligase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following Hars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODi144348	XM_013020444.1 Latest version!	Dipodomys ordii histidyl-tRNA synthetase (Hars), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hars ( XM_013020444.1 ) cDNA ORF clone, Dipodomys ordii(Ord's kangaroo rat) -> XP_012875898.1 Dipodomys ordii histidyl-tRNA synthetase (Hars), mRNA.

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CloneID	ODi144348
Clone ID Related Accession (Same CDS sequence)	XM_013020444.1
Accession Version	XM_013020444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-25
Organism	Dipodomys ordii(Ord's kangaroo rat)
Product	histidine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012267266.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dipodomys ordii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATC GTGCAGAGCT GGAGGAACTG GTGCGGCTTC AGGGAGAGCG CGTGCGGAGC 
CTCAAGCAGC AGAAAGCCAG CGCTGATCAG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGCCC TGATGAAAGC AAACAAAAGT TTGTGCTCAA AACACCCAAG 
GGCACAAGAG ACTACAGTCC CAAGCATATG GCAGTTCGGG AGAAAGTGTT TGATGTAATC 
ATCCGCTGCT TCAAACGCCA TGGTGCAGAA GTGATTGATA CACCTGTGTT TGAACTAAAG 
GAAACACTGA CTGGAAAATA TGGAGAAGAT TCGAAGCTTA TTTATGACCT GAAGGACCAG 
GGAGGGGAGC TGCTGTCCCT TCGTTATGAT CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CATATAGCAA AAGTATATCG GCGGGATAAC 
CCAGCCATGA CCCGAGGCCG ATACCGGGAA TTCTACCAAT GTGATTTTGA CATTGCTGGA 
CAATTTGACC CTATGATCCC TGATGCAGAA TGCCTGAAAA TCATGTGTGA AATCCTGAGT 
TCACTTCAGA TAGGCAACTT CCTGGTCAAG GTAAATGACC GGCGCATATT AGATGGGATG 
TTTGCCGTCT GTGGTGTTCC AGATAGCAAG TTCCGTACCA TCTGTTCCTC AGTAGACAAG 
CTGGATAAGG TATCCTGGGA GGAAGTGAAG AATGAGATGG TGGGAGAGAA GGGACTTGCA 
CCTGAGGTGG CTGACCGCAT CGGAGACTAT GTTCAGCAGC ATGGTGGGGT GTCCCTAGTG 
GAGGAGCTGC TCCAAGATCC TAAACTATCT CAAAACAAGC AGGCCTTAGA GGGCCTGGGA 
GACCTGAAGC TGCTGTTTGA GTACCTGACT CTGTTTGGCA TTGATGACAA GATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTAGACTAT TATACGGGGG TGATCTATGA GGCTGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGACGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGGCGCTATG ATGGACTAGT GGGCATGTTT GACCCTAAAG GGCGCAAGGT ACCATGTGTG 
GGAGTCAGTA TTGGAGTGGA GCGCATCTTC TCCATTGTGG AACAAAGACT GGAGGCTTTA 
GAAGAGAAAG TACGAACCAC AGAGACACAG GTGCTTGTTG CATCTGCGCA AAAGAAGCTG 
CTGGAGGAGA GACTGAAACT TATCTCAGAG CTGTGGGATG CTGGAATCAA GGCTGAACTG 
CTCTACAAGA AGAACCCAAA GTTACTGAAC CAGTTGCAGT ACTGTGAGGA AACAGGTATC 
CCACTAGTGG CCATCATCGG TGAGCAGGAG CTCAAGGATG GGGTCATCAA GCTCCGTTCG 
GTGGCTAGCA GAGAAGAGGT GGATGTCAGA AGAGAAGACC TTGTGGAAGA GATCAGAAGG 
AGAACAAACA AGACCCCTTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012875898.1
CDS	140..1669
Translation	MADRAELEELVRLQGERVRSLKQQKASADQIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPKHMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGQFDPMIPDAECLKIMCEILSSLQIGNFLVKVNDRRILDGMFAVCGVPDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEELLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGDEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGVSIGVERIFSIVEQRLEALEEKVRTTETQVLVASAQKKLLEERLKLISELWDAGIKAELLYKKNPKLLNQLQYCEETGIPLVAIIGEQELKDGVIKLRSVASREEVDVRREDLVEEIRRRTNKTPCIC

Target ORF information:

RefSeq Version	XM_013020444.1
Organism	Dipodomys ordii(Ord's kangaroo rat)
Definition	Dipodomys ordii histidyl-tRNA synthetase (Hars), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013020444.1

ORF Insert Sequence:

ATGGCAGATC GTGCAGAGCT GGAGGAACTG GTGCGGCTTC AGGGAGAGCG CGTGCGGAGC 
CTCAAGCAGC AGAAAGCCAG CGCTGATCAG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGCCC TGATGAAAGC AAACAAAAGT TTGTGCTCAA AACACCCAAG 
GGCACAAGAG ACTACAGTCC CAAGCATATG GCAGTTCGGG AGAAAGTGTT TGATGTAATC 
ATCCGCTGCT TCAAACGCCA TGGTGCAGAA GTGATTGATA CACCTGTGTT TGAACTAAAG 
GAAACACTGA CTGGAAAATA TGGAGAAGAT TCGAAGCTTA TTTATGACCT GAAGGACCAG 
GGAGGGGAGC TGCTGTCCCT TCGTTATGAT CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CATATAGCAA AAGTATATCG GCGGGATAAC 
CCAGCCATGA CCCGAGGCCG ATACCGGGAA TTCTACCAAT GTGATTTTGA CATTGCTGGA 
CAATTTGACC CTATGATCCC TGATGCAGAA TGCCTGAAAA TCATGTGTGA AATCCTGAGT 
TCACTTCAGA TAGGCAACTT CCTGGTCAAG GTAAATGACC GGCGCATATT AGATGGGATG 
TTTGCCGTCT GTGGTGTTCC AGATAGCAAG TTCCGTACCA TCTGTTCCTC AGTAGACAAG 
CTGGATAAGG TATCCTGGGA GGAAGTGAAG AATGAGATGG TGGGAGAGAA GGGACTTGCA 
CCTGAGGTGG CTGACCGCAT CGGAGACTAT GTTCAGCAGC ATGGTGGGGT GTCCCTAGTG 
GAGGAGCTGC TCCAAGATCC TAAACTATCT CAAAACAAGC AGGCCTTAGA GGGCCTGGGA 
GACCTGAAGC TGCTGTTTGA GTACCTGACT CTGTTTGGCA TTGATGACAA GATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTAGACTAT TATACGGGGG TGATCTATGA GGCTGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGACGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGGCGCTATG ATGGACTAGT GGGCATGTTT GACCCTAAAG GGCGCAAGGT ACCATGTGTG 
GGAGTCAGTA TTGGAGTGGA GCGCATCTTC TCCATTGTGG AACAAAGACT GGAGGCTTTA 
GAAGAGAAAG TACGAACCAC AGAGACACAG GTGCTTGTTG CATCTGCGCA AAAGAAGCTG 
CTGGAGGAGA GACTGAAACT TATCTCAGAG CTGTGGGATG CTGGAATCAA GGCTGAACTG 
CTCTACAAGA AGAACCCAAA GTTACTGAAC CAGTTGCAGT ACTGTGAGGA AACAGGTATC 
CCACTAGTGG CCATCATCGG TGAGCAGGAG CTCAAGGATG GGGTCATCAA GCTCCGTTCG 
GTGGCTAGCA GAGAAGAGGT GGATGTCAGA AGAGAAGACC TTGTGGAAGA GATCAGAAGG 
AGAACAAACA AGACCCCTTG CATCTGCTGA