LOC109545487 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109545487
Entrez Gene ID	109545487
Full Name	V-type proton ATPase subunit B
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019916227.1	XP_019771786.1	V-type proton ATPase subunit B

Pathways from BioSystems
Gene Ontology

The following LOC109545487 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109545487 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe50470	XM_019916227.1 Latest version!	Dendroctonus ponderosae V-type proton ATPase subunit B (LOC109545487), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109545487 ( XM_019916227.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019771786.1 Dendroctonus ponderosae V-type proton ATPase subunit B (LOC109545487), mRNA.

Price & Availability↑

CloneID	ODe50470
Clone ID Related Accession (Same CDS sequence)	XM_019916227.1
Accession Version	XM_019916227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	V-type proton ATPase subunit B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTAT CTGCAAGACA GGCCAGCCGG GAACACGTTC TAGCTGTTTC CCGAGACTTT 
GTGTCCCAAC CAAGATTGAC CTATAAAACA GTTTCTGGAG TCAACGGACC GCTGGTCATC 
TTGGAGGATG TCAAGTTCCC GAAATTCAAC GAAATCGTCG AACTTAAACT GGCTGATGGA 
AGCGCCAGGT CTGGACAAGT CCTGGAAGTC AGTGGATCCA AAGCTGTGGT CCAAGTGTTC 
GAGGGAACAT CAGGAATTGA TGCCAAACAC ACCCTCTGCG AGTTCACGGG GGATATTTTG 
AGAACCCCTG TATCTGAAGA CATGTTAGGG CGTGTGTTCA ATGGATCTGG CAAACCCATC 
GACAAGGGAC CTCCAATTTT GGCGGAAGAT TACTTGGACA TCCAAGGTCA GCCCATCAAC 
CCCTGGTCGC GTATCTACCC AGAAGAAATG ATCCAAACTG GTATCTCCGC CATTGATGTG 
ATGAACTCTA TTGCTCGTGG CCAGAAGATC CCCATTTTCT CGGCTGCTGG TCTGCCGCAC 
AATGAAATCG CCGCCCAAAT CTGTAGACAA GCCGGTCTTG TTAAGGTGCC CGGAAAATCC 
GTGATGGATG ATCATGAAGA TAATTTTGCC ATTGTTTTCG CTGCTATGGG TGTGAACATG 
GAAACCGCTA GATTCTTCAA GCAAGATTTC GAAGAAAACG GCTCCATGGA GAACGTGTGC 
CTGTTCTTGA ACTTGGCTAA TGACCCGACC ATTGAAAGAA TCATCACGCC TCGTCTGGCT 
TTGACTGCGG CCGAATTCTT GGCCTATCAA TGCGAAAAAC ACGTCCTGGT CATCTTGACT 
GACATGTCTT CATATGCTGA AGCCTTGCGT GAGGTATCAG CTGCTAGAGA AGAAGTGCCC 
GGTCGTCGTG GTTTTCCCGG TTACATGTAC ACCGATTTGG CCACTATTTA TGAGCGTGCT 
GGGCGTGTTG AGGGCAGGAA CGGCTCCATT ACTCAAATCC CCATCTTGAC TATGCCTAAC 
GATGATATTA CGCATCCAAT CCCCGATTTG ACTGGATACA TTACTGAAGG TCAAATCTAC 
GTCGACAGGC AGCTGCACAA CAGGCAGATT TATCCTCCAA TCAACGTGCT GCCATCGTTG 
TCACGTTTGA TGAAGTCCGC TATTGGTGAG GACATGACCA GGAAGGATCA TTCTGATGTT 
TCCAACCAGT TGTATGCTTG CTACGCTATT GGAAAGGACG TCCAAGCCAT GAAAGCTGTT 
GTGGGTGAAG AAGCTTTGAC TCCCGATGAT CTTCTCTACC TCGAGTTCCT GACCAAGTTC 
GAAAAGAACT TCATCTCACA AGGAAACTAT GAAAACAGGA CGGTGTTCGA GTCGTTGGAC 
ATTGGCTGGC AATTGCTGCG TATCTTCCCC AAGGAGATGT TGAAACGTAT CCCAGCAGCC 
ACTCTTGCGG AATTCTATCC CAGAGATTCG CGTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019771786.1
CDS	169..1647
Translation	MSLSARQASREHVLAVSRDFVSQPRLTYKTVSGVNGPLVILEDVKFPKFNEIVELKLADGSARSGQVLEVSGSKAVVQVFEGTSGIDAKHTLCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPPILAEDYLDIQGQPINPWSRIYPEEMIQTGISAIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKVPGKSVMDDHEDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERIITPRLALTAAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEDMTRKDHSDVSNQLYACYAIGKDVQAMKAVVGEEALTPDDLLYLEFLTKFEKNFISQGNYENRTVFESLDIGWQLLRIFPKEMLKRIPAATLAEFYPRDSRH

Target ORF information:

RefSeq Version	XM_019916227.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae V-type proton ATPase subunit B (LOC109545487), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019916227.1

ORF Insert Sequence:

ATGTCTTTAT CTGCAAGACA GGCCAGCCGG GAACACGTTC TAGCTGTTTC CCGAGACTTT 
GTGTCCCAAC CAAGATTGAC CTATAAAACA GTTTCTGGAG TCAACGGACC GCTGGTCATC 
TTGGAGGATG TCAAGTTCCC GAAATTCAAC GAAATCGTCG AACTTAAACT GGCTGATGGA 
AGCGCCAGGT CTGGACAAGT CCTGGAAGTC AGTGGATCCA AAGCTGTGGT CCAAGTGTTC 
GAGGGAACAT CAGGAATTGA TGCCAAACAC ACCCTCTGCG AGTTCACGGG GGATATTTTG 
AGAACCCCTG TATCTGAAGA CATGTTAGGG CGTGTGTTCA ATGGATCTGG CAAACCCATC 
GACAAGGGAC CTCCAATTTT GGCGGAAGAT TACTTGGACA TCCAAGGTCA GCCCATCAAC 
CCCTGGTCGC GTATCTACCC AGAAGAAATG ATCCAAACTG GTATCTCCGC CATTGATGTG 
ATGAACTCTA TTGCTCGTGG CCAGAAGATC CCCATTTTCT CGGCTGCTGG TCTGCCGCAC 
AATGAAATCG CCGCCCAAAT CTGTAGACAA GCCGGTCTTG TTAAGGTGCC CGGAAAATCC 
GTGATGGATG ATCATGAAGA TAATTTTGCC ATTGTTTTCG CTGCTATGGG TGTGAACATG 
GAAACCGCTA GATTCTTCAA GCAAGATTTC GAAGAAAACG GCTCCATGGA GAACGTGTGC 
CTGTTCTTGA ACTTGGCTAA TGACCCGACC ATTGAAAGAA TCATCACGCC TCGTCTGGCT 
TTGACTGCGG CCGAATTCTT GGCCTATCAA TGCGAAAAAC ACGTCCTGGT CATCTTGACT 
GACATGTCTT CATATGCTGA AGCCTTGCGT GAGGTATCAG CTGCTAGAGA AGAAGTGCCC 
GGTCGTCGTG GTTTTCCCGG TTACATGTAC ACCGATTTGG CCACTATTTA TGAGCGTGCT 
GGGCGTGTTG AGGGCAGGAA CGGCTCCATT ACTCAAATCC CCATCTTGAC TATGCCTAAC 
GATGATATTA CGCATCCAAT CCCCGATTTG ACTGGATACA TTACTGAAGG TCAAATCTAC 
GTCGACAGGC AGCTGCACAA CAGGCAGATT TATCCTCCAA TCAACGTGCT GCCATCGTTG 
TCACGTTTGA TGAAGTCCGC TATTGGTGAG GACATGACCA GGAAGGATCA TTCTGATGTT 
TCCAACCAGT TGTATGCTTG CTACGCTATT GGAAAGGACG TCCAAGCCAT GAAAGCTGTT 
GTGGGTGAAG AAGCTTTGAC TCCCGATGAT CTTCTCTACC TCGAGTTCCT GACCAAGTTC 
GAAAAGAACT TCATCTCACA AGGAAACTAT GAAAACAGGA CGGTGTTCGA GTCGTTGGAC 
ATTGGCTGGC AATTGCTGCG TATCTTCCCC AAGGAGATGT TGAAACGTAT CCCAGCAGCC 
ACTCTTGCGG AATTCTATCC CAGAGATTCG CGTCATTAA