LOC109539064 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109539064
Entrez Gene ID	109539064
Full Name	glycogen debranching enzyme
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019906636.1	XP_019762195.1	glycogen debranching enzyme isoform X5
XM_019906633.1	XP_019762192.1	glycogen debranching enzyme isoform X2
XM_019906634.1	XP_019762193.1	glycogen debranching enzyme isoform X3
XM_019906635.1	XP_019762194.1	glycogen debranching enzyme isoform X4
XM_019906637.1	XP_019762196.1	glycogen debranching enzyme isoform X6
XM_019906632.1	XP_019762191.1	glycogen debranching enzyme isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC109539064 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109539064 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe42977	XM_019906632.1 Latest version!	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODe42978	XM_019906633.1 Latest version!	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODe42979	XM_019906634.1 Latest version!	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODe42980	XM_019906635.1 Latest version!	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODe42980	XM_019906636.1 Latest version!	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODe42981	XM_019906637.1 Latest version!	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109539064 ( XM_019906632.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019762191.1 Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODe42977
Clone ID Related Accession (Same CDS sequence)	XM_019906632.1
Accession Version	XM_019906632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTG TAAATATATT GATACTGAAC GATAAAGCAA ACTTGGATTC CGAGCTTCAC 
AGAATTCAAA AAAATGTTAA ATTGGAGTTC AGACTCGGCC CTTCTTTGCT GGGCAGGAAT 
ATAAAGCTGT ATTGCAACTA TCCGAAGTCA ACTAACGGAA TTGTGGAAGA ATTCAGCAGA 
GATTGTTATA CGTTAATAAG ATGGTCACGG AATGAAGGAT GCAAAAACGC AGACGGTAGC 
TCAACCTTTG CACAGATCAC CCCCACGATC GCAGGAAGCT TCCACTACTA CTTCGTTGAA 
GACGATGAGT TTACTAAGAC GAATTTCAAA AACGGATCCG GCTATTTTAT AGTTGACCCT 
GTATTGAAAT ATGGTGCCAA TGAGCTGTTG CCTCTGAACT GTATACAGAG TCAAACGGTA 
TTGTCAAAAC TTTTGGGGCC GTTCTCCACA TGGGAAAACA AGATACGGGT TGCCAAAGAA 
TCCGGTTATA ACATGGTGCA TTTTACGCCC ATTCAGCAAC TAGGAGGATC CCATTCCGCA 
TACAGTGTCC AAGACCACTT GAAATTAAAT GTTTCTTTTA ACGAAGAAAA TGGACGAGAA 
CCGTCATTTG AAGATATCGA GACGCTGACC GCAAAAATGC GTGAAGATTG GAAGATGTTG 
TCCGTATGTG ACATAGTGAT AAATCACACA GCCAATGAAA GTGATTGGAT TAAAGAACAC 
CCTGAAGCAG CATACAATTG CTTGAACTGT CCTTACATGC GACCAGCCTA TCTGTTAGAT 
GAAGCTCTCC ATCAGTTTTC CATGGATATA AGAAAAGGCC TCTATGAGGA CCGGGGAATC 
CCTCTTGACG TTTCAACGGA AGATCATCTA AACGCAATCC GTTATCACTT TAAACAAAAC 
GTGTTACAAC CATTGAAAAT TCATGAGCTG TTTATTGTAG ACGTAACCCA ATACTTGGAA 
CAGTTTTTAA CACAGGCCAG AAAAACCGCA CCTCTCAATG ACTGCGAAAG AAACCTCTTA 
CCGGACTTGA AAATTATTCA AGATGTTCAG TTTAGGAGAT TGAGCAGTTC GGTCGACTTA 
AATTTGGCCC TCGCCAAGTT CAACTGCTAT CGGCCAGATT GTTACGATGA GGAGTCGAGA 
GTTAGAAGAT GTTCCGAGGA TTTTAAAATT CAGCTGGATG CCCTCAATCA GGCTGCAATT 
GATGAAGTCA CTGCTGACCT TAGTGCTGCA GTTGAAAACG TTATTGCTGG CATTCGGTAT 
TTCAGAGTAC AGCCAGACGG CCCTCAATAT AAGGCCATTA CCATTGATCA TCCATTGGTG 
TACAGGTACT TTACCGATTA TCATTCACCC AAATCCCTGG CAGAAGCTGA AGAAACAATG 
TACAGTACAA ACGGACGGTT TCTCATGGCC CATAATGGTT GGGTGATGAG CGCTGAGCCA 
TTAAAGAACT TTGCCGATGC CGGCACCAAG ATTTACATTA GAAGGGAACT TATCGCTTGG 
GGCGACAGCG TTAAGCTACG CTTCGGGGAC AAGCCGGCCG ATTCTCCATT TTTGTGGAAT 
TACATGCGGC AGTACGTAGA ACAGACGGTT AAAATATTTG ATGGTATTCG GCTGGACAAC 
TGCCACTCAA CTTCCCTTCC CGTTGCCGAA TATCTTTTGG ACTGCGCCAG AAGAATCCGT 
CCTGATCTTT ATGTGGTGGC CGAGTTGTTC ACCAACTCGG CCTTGACTGA CAACATTTTC 
GTAAATCGTT TGGGCATCAC GTCGCTTATT CGAGAAGCGA TGTCCGCATG GGATGCTCAT 
GAACAGGGAC GTCTGGTATA TCGATTCGGA GGTGTTCCAG TGGGTGCTTT TTACAAACCT 
TCAAAAGTAC CATTGGTCCC TATGGTGGCA CATGCACTTT TCCTCGATCA AACCCATGAT 
AATCCCAGTC CAGTCGAAAA AAGATCTGTT TTCGATTTGC TGCCGAGCGC CGGTTTAGTA 
AACATGGCAT GTTGTGCCAC CGGCTCAAAT CGAGGCTACG ACGAACTGGT TCCCCACCAC 
ATCCACGTAG TGGATGAAAC TAGACAATAC GCTGAGTGGA GTCATCATGA AAAGCTTCCT 
TCCAAAACCA GATATGTTAA TAAGTGGTCA GGCATTATTG CGGCCAAAAG AGCTCTGCAC 
GAGTTGCACT TTAAGCTAGG AATGGAAGGA TTCGACCAGG TATTTGTGAA TCAAGTAGAT 
GGCGATATTG TTGAGGTGAC CAGGCACCAA CCGGGCACCC ACACCAGTTA TGTCCTAGTA 
GCATTCACGG CCTTTGAACA TCCCACCGGT GACCACCAGA GAGCCATTAA ACCGCTTTCT 
GTTGAAGGAG TTCTGGATGA AATTGTACTG GAAGCCTACT TAATTCATGC AGATTCCACG 
TTTGGCAGAT TTGAAAAATT TGAGAAAGAC CCCGATTGGA TCAACGGATT AGCCGAATAT 
AAGCTTTCAA TTAAAGAGCA CATCCAGTTG CGCGAATCTG AGTTTTTCGA GGAAGCAGCT 
GACAGTAGTT CTAATTTCGT CCGGTTAAAC TATAAAAACT TCAAGCCCGG ATCAATTGCA 
GTGTTGAAAT TTTCTTTGCC CCAAGGAATC CGAGATGCGA TATCTCTGTT GAAACCCCCA 
CAGGCTCCAA ATCTAGAGAA AATTGTAAGT GAATTGACTC TAGCAGATTT GAACAGAACT 
CTCTTTCGAT GTGGCGAAGA AGAAAGGGAT GAAGGCAATG GCTTCGACAC CTACAACATA 
CCCAACTTTG GACCGACAAT TTATTGTGGT TTATTCGGCA TTACGTGGGT GCTGCGTCAA 
ATAGCCACAG ACAATGACCT GGGCCATCCA TTGTGTACCA ACTTGCGAAA TGGCAACTGG 
TTGATTGAAT ACGTTTTCAA ACGCCTTAAA GCCGATGAGG GGACCCGCGC TTTGGGGGAA 
TGGATTGAAG ACTACAGTAA GCCCCTCCTA AATATGCCCC GTTACTTAGT TCCCTGTTAT 
TTCGATGTCT TCATTGGGCA AATATACCCT CAGCTACTAA ATGCTGGCTA TCAATTGATG 
TCTAATTTTA TCAAGCAGGG ATCGCCTTTT GTTCAGTCGT TGGCTTTGGG GTCGTTGCAA 
TTTGGCGGTT ACCTCAGATC TGCCTGTCTG CCAGATATGT CGCCCAATCT GGCTCCGCCA 
AAACCTCCTA GCAGAAAAAA CGAAAACGGT GAAACAGTCC AGGCCTGCGT TACTTTATCA 
GCAGGATTGC CCCACTTCGC CGTGGGTTAC ATGCGAAATT GGGGTAGAGA CACCTTTATT 
GCTCTGCGCG GGCTGTTCAT CTTAACTGGC CGGTTTGAAG AAGCCCGACA ACATATTCTG 
GGTTACGGGT CGTGTTTGAG GCACGGCCTC ATTCCGAATT TACTGGATGC TGGTAGAAAT 
CCCAGGTTTA ACTGCCGAGA TGCCATTTGG TGGTGGCTCT ATTCGCTCAA ATCATATTGC 
GAAGAATCCC CTAAGGGCGT TGCTATTCTT GCCGATGTTG TATCACGCAT ATTTCCTTCG 
GATGATTCTT CTCCACAACC GCCTGGTGCT GTGGATCAAC CGCTATACAA TGTAATTCAG 
GAATCGTTAA CTGTCCATTT CCAAGGCCTA CAATTTCGCG AAAGGAACGC TGGACGAGAA 
ATTGATGAAC ACATGACCGA TGCAGGATTT AACAACCAAA TCGGAACTCA TCCGGAAACT 
GGATTCGTTT TTGGTGGGAA TGAATGGAAT TGCGGTACTT GGATGGACAA AATGGGTTCA 
TCAGACAAGG CTGGCAATCG GGGCAAACCG GCGTCGCCTC GTGACGGATC TGCTGTCGAA 
CTCGTAGGCT TATCTAAAGC CTGTATCACT TGGTTAATGC ACTTAAATGA TGAAGGTCAG 
TACCCTTATG ATGGGGTTCA GCGAACACAT AAGAATGGAA CGGTGACCAA ATGGACCTTC 
AGAGAATGGT CGGACAAAAT TCAACAAAAC TTCGAAAAGA AATTCTGGAT TTCAGGGAAC 
CCGAACCCAG AAGAATGTAG GCCTGATTTA GTGAATAAGA AGAACATTTA CAAAGACAGC 
TTCGGAGCGT CTCAGCCATG GACAGATTTT CAGCTTAGGT GTAATTTTCC CATTGCCATG 
GTCGCTGCTC CCGAGTTGTT TAATCCTCAA CGTGCATGGG CCGCATTAGA GCAAGTCGAA 
AAGTATCTCT TGGGGCCACT AGGCATGAAA ACGCTAGACC CCGAAGACTG GAATTACCGG 
GGCGACTATG ATAATTCGAT TGATTCAGAT GATTTACTTA CGGCCCATGG CTTCAATTAT 
CATCAAGGCC CTGAATGGGT TTGGCCTGTG GGTTTTTTCC TAAGAGCAAA GTTAATATTT 
GCTGCTCAGA ATGGACTGTT AGACGAAACA GTGACCAAAA CAAAACAAAT CTTATCAAAG 
CATTTTATCG AAGTGGAGAC GCCACCGTGG CGTGGATTAC CGGAATTAAC CAACAGCGAT 
GGATCCTACT GCCCGGACAG TGCGAGAACC CAAGCTTGGA GCATGTCCTG CATTCTCGAG 
GTCCTGTATG ACTTGGAGAA AATCGAATCC AATAGAAGTG TTGCAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019762191.1
CDS	517..5127
Translation	MASVNILILNDKANLDSELHRIQKNVKLEFRLGPSLLGRNIKLYCNYPKSTNGIVEEFSRDCYTLIRWSRNEGCKNADGSSTFAQITPTIAGSFHYYFVEDDEFTKTNFKNGSGYFIVDPVLKYGANELLPLNCIQSQTVLSKLLGPFSTWENKIRVAKESGYNMVHFTPIQQLGGSHSAYSVQDHLKLNVSFNEENGREPSFEDIETLTAKMREDWKMLSVCDIVINHTANESDWIKEHPEAAYNCLNCPYMRPAYLLDEALHQFSMDIRKGLYEDRGIPLDVSTEDHLNAIRYHFKQNVLQPLKIHELFIVDVTQYLEQFLTQARKTAPLNDCERNLLPDLKIIQDVQFRRLSSSVDLNLALAKFNCYRPDCYDEESRVRRCSEDFKIQLDALNQAAIDEVTADLSAAVENVIAGIRYFRVQPDGPQYKAITIDHPLVYRYFTDYHSPKSLAEAEETMYSTNGRFLMAHNGWVMSAEPLKNFADAGTKIYIRRELIAWGDSVKLRFGDKPADSPFLWNYMRQYVEQTVKIFDGIRLDNCHSTSLPVAEYLLDCARRIRPDLYVVAELFTNSALTDNIFVNRLGITSLIREAMSAWDAHEQGRLVYRFGGVPVGAFYKPSKVPLVPMVAHALFLDQTHDNPSPVEKRSVFDLLPSAGLVNMACCATGSNRGYDELVPHHIHVVDETRQYAEWSHHEKLPSKTRYVNKWSGIIAAKRALHELHFKLGMEGFDQVFVNQVDGDIVEVTRHQPGTHTSYVLVAFTAFEHPTGDHQRAIKPLSVEGVLDEIVLEAYLIHADSTFGRFEKFEKDPDWINGLAEYKLSIKEHIQLRESEFFEEAADSSSNFVRLNYKNFKPGSIAVLKFSLPQGIRDAISLLKPPQAPNLEKIVSELTLADLNRTLFRCGEEERDEGNGFDTYNIPNFGPTIYCGLFGITWVLRQIATDNDLGHPLCTNLRNGNWLIEYVFKRLKADEGTRALGEWIEDYSKPLLNMPRYLVPCYFDVFIGQIYPQLLNAGYQLMSNFIKQGSPFVQSLALGSLQFGGYLRSACLPDMSPNLAPPKPPSRKNENGETVQACVTLSAGLPHFAVGYMRNWGRDTFIALRGLFILTGRFEEARQHILGYGSCLRHGLIPNLLDAGRNPRFNCRDAIWWWLYSLKSYCEESPKGVAILADVVSRIFPSDDSSPQPPGAVDQPLYNVIQESLTVHFQGLQFRERNAGREIDEHMTDAGFNNQIGTHPETGFVFGGNEWNCGTWMDKMGSSDKAGNRGKPASPRDGSAVELVGLSKACITWLMHLNDEGQYPYDGVQRTHKNGTVTKWTFREWSDKIQQNFEKKFWISGNPNPEECRPDLVNKKNIYKDSFGASQPWTDFQLRCNFPIAMVAAPELFNPQRAWAALEQVEKYLLGPLGMKTLDPEDWNYRGDYDNSIDSDDLLTAHGFNYHQGPEWVWPVGFFLRAKLIFAAQNGLLDETVTKTKQILSKHFIEVETPPWRGLPELTNSDGSYCPDSARTQAWSMSCILEVLYDLEKIESNRSVAN

Target ORF information:

RefSeq Version	XM_019906632.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019906632.1

ORF Insert Sequence:

ATGGCATCTG TAAATATATT GATACTGAAC GATAAAGCAA ACTTGGATTC CGAGCTTCAC 
AGAATTCAAA AAAATGTTAA ATTGGAGTTC AGACTCGGCC CTTCTTTGCT GGGCAGGAAT 
ATAAAGCTGT ATTGCAACTA TCCGAAGTCA ACTAACGGAA TTGTGGAAGA ATTCAGCAGA 
GATTGTTATA CGTTAATAAG ATGGTCACGG AATGAAGGAT GCAAAAACGC AGACGGTAGC 
TCAACCTTTG CACAGATCAC CCCCACGATC GCAGGAAGCT TCCACTACTA CTTCGTTGAA 
GACGATGAGT TTACTAAGAC GAATTTCAAA AACGGATCCG GCTATTTTAT AGTTGACCCT 
GTATTGAAAT ATGGTGCCAA TGAGCTGTTG CCTCTGAACT GTATACAGAG TCAAACGGTA 
TTGTCAAAAC TTTTGGGGCC GTTCTCCACA TGGGAAAACA AGATACGGGT TGCCAAAGAA 
TCCGGTTATA ACATGGTGCA TTTTACGCCC ATTCAGCAAC TAGGAGGATC CCATTCCGCA 
TACAGTGTCC AAGACCACTT GAAATTAAAT GTTTCTTTTA ACGAAGAAAA TGGACGAGAA 
CCGTCATTTG AAGATATCGA GACGCTGACC GCAAAAATGC GTGAAGATTG GAAGATGTTG 
TCCGTATGTG ACATAGTGAT AAATCACACA GCCAATGAAA GTGATTGGAT TAAAGAACAC 
CCTGAAGCAG CATACAATTG CTTGAACTGT CCTTACATGC GACCAGCCTA TCTGTTAGAT 
GAAGCTCTCC ATCAGTTTTC CATGGATATA AGAAAAGGCC TCTATGAGGA CCGGGGAATC 
CCTCTTGACG TTTCAACGGA AGATCATCTA AACGCAATCC GTTATCACTT TAAACAAAAC 
GTGTTACAAC CATTGAAAAT TCATGAGCTG TTTATTGTAG ACGTAACCCA ATACTTGGAA 
CAGTTTTTAA CACAGGCCAG AAAAACCGCA CCTCTCAATG ACTGCGAAAG AAACCTCTTA 
CCGGACTTGA AAATTATTCA AGATGTTCAG TTTAGGAGAT TGAGCAGTTC GGTCGACTTA 
AATTTGGCCC TCGCCAAGTT CAACTGCTAT CGGCCAGATT GTTACGATGA GGAGTCGAGA 
GTTAGAAGAT GTTCCGAGGA TTTTAAAATT CAGCTGGATG CCCTCAATCA GGCTGCAATT 
GATGAAGTCA CTGCTGACCT TAGTGCTGCA GTTGAAAACG TTATTGCTGG CATTCGGTAT 
TTCAGAGTAC AGCCAGACGG CCCTCAATAT AAGGCCATTA CCATTGATCA TCCATTGGTG 
TACAGGTACT TTACCGATTA TCATTCACCC AAATCCCTGG CAGAAGCTGA AGAAACAATG 
TACAGTACAA ACGGACGGTT TCTCATGGCC CATAATGGTT GGGTGATGAG CGCTGAGCCA 
TTAAAGAACT TTGCCGATGC CGGCACCAAG ATTTACATTA GAAGGGAACT TATCGCTTGG 
GGCGACAGCG TTAAGCTACG CTTCGGGGAC AAGCCGGCCG ATTCTCCATT TTTGTGGAAT 
TACATGCGGC AGTACGTAGA ACAGACGGTT AAAATATTTG ATGGTATTCG GCTGGACAAC 
TGCCACTCAA CTTCCCTTCC CGTTGCCGAA TATCTTTTGG ACTGCGCCAG AAGAATCCGT 
CCTGATCTTT ATGTGGTGGC CGAGTTGTTC ACCAACTCGG CCTTGACTGA CAACATTTTC 
GTAAATCGTT TGGGCATCAC GTCGCTTATT CGAGAAGCGA TGTCCGCATG GGATGCTCAT 
GAACAGGGAC GTCTGGTATA TCGATTCGGA GGTGTTCCAG TGGGTGCTTT TTACAAACCT 
TCAAAAGTAC CATTGGTCCC TATGGTGGCA CATGCACTTT TCCTCGATCA AACCCATGAT 
AATCCCAGTC CAGTCGAAAA AAGATCTGTT TTCGATTTGC TGCCGAGCGC CGGTTTAGTA 
AACATGGCAT GTTGTGCCAC CGGCTCAAAT CGAGGCTACG ACGAACTGGT TCCCCACCAC 
ATCCACGTAG TGGATGAAAC TAGACAATAC GCTGAGTGGA GTCATCATGA AAAGCTTCCT 
TCCAAAACCA GATATGTTAA TAAGTGGTCA GGCATTATTG CGGCCAAAAG AGCTCTGCAC 
GAGTTGCACT TTAAGCTAGG AATGGAAGGA TTCGACCAGG TATTTGTGAA TCAAGTAGAT 
GGCGATATTG TTGAGGTGAC CAGGCACCAA CCGGGCACCC ACACCAGTTA TGTCCTAGTA 
GCATTCACGG CCTTTGAACA TCCCACCGGT GACCACCAGA GAGCCATTAA ACCGCTTTCT 
GTTGAAGGAG TTCTGGATGA AATTGTACTG GAAGCCTACT TAATTCATGC AGATTCCACG 
TTTGGCAGAT TTGAAAAATT TGAGAAAGAC CCCGATTGGA TCAACGGATT AGCCGAATAT 
AAGCTTTCAA TTAAAGAGCA CATCCAGTTG CGCGAATCTG AGTTTTTCGA GGAAGCAGCT 
GACAGTAGTT CTAATTTCGT CCGGTTAAAC TATAAAAACT TCAAGCCCGG ATCAATTGCA 
GTGTTGAAAT TTTCTTTGCC CCAAGGAATC CGAGATGCGA TATCTCTGTT GAAACCCCCA 
CAGGCTCCAA ATCTAGAGAA AATTGTAAGT GAATTGACTC TAGCAGATTT GAACAGAACT 
CTCTTTCGAT GTGGCGAAGA AGAAAGGGAT GAAGGCAATG GCTTCGACAC CTACAACATA 
CCCAACTTTG GACCGACAAT TTATTGTGGT TTATTCGGCA TTACGTGGGT GCTGCGTCAA 
ATAGCCACAG ACAATGACCT GGGCCATCCA TTGTGTACCA ACTTGCGAAA TGGCAACTGG 
TTGATTGAAT ACGTTTTCAA ACGCCTTAAA GCCGATGAGG GGACCCGCGC TTTGGGGGAA 
TGGATTGAAG ACTACAGTAA GCCCCTCCTA AATATGCCCC GTTACTTAGT TCCCTGTTAT 
TTCGATGTCT TCATTGGGCA AATATACCCT CAGCTACTAA ATGCTGGCTA TCAATTGATG 
TCTAATTTTA TCAAGCAGGG ATCGCCTTTT GTTCAGTCGT TGGCTTTGGG GTCGTTGCAA 
TTTGGCGGTT ACCTCAGATC TGCCTGTCTG CCAGATATGT CGCCCAATCT GGCTCCGCCA 
AAACCTCCTA GCAGAAAAAA CGAAAACGGT GAAACAGTCC AGGCCTGCGT TACTTTATCA 
GCAGGATTGC CCCACTTCGC CGTGGGTTAC ATGCGAAATT GGGGTAGAGA CACCTTTATT 
GCTCTGCGCG GGCTGTTCAT CTTAACTGGC CGGTTTGAAG AAGCCCGACA ACATATTCTG 
GGTTACGGGT CGTGTTTGAG GCACGGCCTC ATTCCGAATT TACTGGATGC TGGTAGAAAT 
CCCAGGTTTA ACTGCCGAGA TGCCATTTGG TGGTGGCTCT ATTCGCTCAA ATCATATTGC 
GAAGAATCCC CTAAGGGCGT TGCTATTCTT GCCGATGTTG TATCACGCAT ATTTCCTTCG 
GATGATTCTT CTCCACAACC GCCTGGTGCT GTGGATCAAC CGCTATACAA TGTAATTCAG 
GAATCGTTAA CTGTCCATTT CCAAGGCCTA CAATTTCGCG AAAGGAACGC TGGACGAGAA 
ATTGATGAAC ACATGACCGA TGCAGGATTT AACAACCAAA TCGGAACTCA TCCGGAAACT 
GGATTCGTTT TTGGTGGGAA TGAATGGAAT TGCGGTACTT GGATGGACAA AATGGGTTCA 
TCAGACAAGG CTGGCAATCG GGGCAAACCG GCGTCGCCTC GTGACGGATC TGCTGTCGAA 
CTCGTAGGCT TATCTAAAGC CTGTATCACT TGGTTAATGC ACTTAAATGA TGAAGGTCAG 
TACCCTTATG ATGGGGTTCA GCGAACACAT AAGAATGGAA CGGTGACCAA ATGGACCTTC 
AGAGAATGGT CGGACAAAAT TCAACAAAAC TTCGAAAAGA AATTCTGGAT TTCAGGGAAC 
CCGAACCCAG AAGAATGTAG GCCTGATTTA GTGAATAAGA AGAACATTTA CAAAGACAGC 
TTCGGAGCGT CTCAGCCATG GACAGATTTT CAGCTTAGGT GTAATTTTCC CATTGCCATG 
GTCGCTGCTC CCGAGTTGTT TAATCCTCAA CGTGCATGGG CCGCATTAGA GCAAGTCGAA 
AAGTATCTCT TGGGGCCACT AGGCATGAAA ACGCTAGACC CCGAAGACTG GAATTACCGG 
GGCGACTATG ATAATTCGAT TGATTCAGAT GATTTACTTA CGGCCCATGG CTTCAATTAT 
CATCAAGGCC CTGAATGGGT TTGGCCTGTG GGTTTTTTCC TAAGAGCAAA GTTAATATTT 
GCTGCTCAGA ATGGACTGTT AGACGAAACA GTGACCAAAA CAAAACAAAT CTTATCAAAG 
CATTTTATCG AAGTGGAGAC GCCACCGTGG CGTGGATTAC CGGAATTAAC CAACAGCGAT 
GGATCCTACT GCCCGGACAG TGCGAGAACC CAAGCTTGGA GCATGTCCTG CATTCTCGAG 
GTCCTGTATG ACTTGGAGAA AATCGAATCC AATAGAAGTG TTGCAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109539064 ( XM_019906633.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019762192.1 Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODe42978
Clone ID Related Accession (Same CDS sequence)	XM_019906633.1
Accession Version	XM_019906633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGTTT TAGGTGCGTG TTTATGTTTG GTTGCTATTC TTCTGCAGAT ATGCTTTTAT 
TTGTGTTCGT TCAGCGCGCA ACAAAAGATC CGATCTAAAA TGGCATCATT GGCATCATGG 
CTCTTCGGCA GAGTTGCAGG TGCCGCACCA GAAAACCAAA CTGACCAGAA CATCGGTCAA 
GCTACAACTT CTGGTAAAAA CATCTCGCAT GCCAGCATAG TCACCAATAC TGTTCCCGTC 
GGTTTTGAGG CTTCTCGTGG TACCTCGGCC CTACCCATCA AAGAACACGA AAGTGCGAGC 
TCTCTTGAAG CATCGGGTGA AACTGCAAAA AGTTTTGACG ATTTCGTGCA CGTAAACATT 
GATGAAGAAA ACGGTGATTC AGAAGGCGGC TTGTATGAAC AGACTTCACA TAGCGATCGA 
ACGCTGAATG ATCCCAATCG GCAGCAAGCA GCCGAACCAA GTTTTACAAA CTGCAGTACC 
GAATGCGTAG ATTTCGAAGT TGAAAACTCT AGTCCTAAAT GTTCACAAAT AGAAACTGTT 
GAAAATTTGA AAATTCCAGA CATTCGACCA GGTGAATGTA AGAGAGAACT AAGCGCCGTT 
GAAAACCGAA AGCTCAACGA CGAGCCGGAA CATCCAAAAT ATATCGACAT GGCATCTGTA 
AATATATTGA TACTGAACGA TAAAGCAAAC TTGGATTCCG AGCTTCACAG AATTCAAAAA 
AATGTTAAAT TGGAGTTCAG ACTCGGCCCT TCTTTGCTGG GCAGGAATAT AAAGCTGTAT 
TGCAACTATC CGAAGTCAAC TAACGGAATT GTGGAAGAAT TCAGCAGAGA TTGTTATACG 
TTAATAAGAT GGTCACGGAA TGAAGGATGC AAAAACGCAG ACGGTAGCTC AACCTTTGCA 
CAGATCACCC CCACGATCGC AGGAAGCTTC CACTACTACT TCGTTGAAGA CGATGAGTTT 
ACTAAGACGA ATTTCAAAAA CGGATCCGGC TATTTTATAG TTGACCCTGT ATTGAAATAT 
GGTGCCAATG AGCTGTTGCC TCTGAACTGT ATACAGAGTC AAACGGTATT GTCAAAACTT 
TTGGGGCCGT TCTCCACATG GGAAAACAAG ATACGGGTTG CCAAAGAATC CGGTTATAAC 
ATGGTGCATT TTACGCCCAT TCAGCAACTA GGAGGATCCC ATTCCGCATA CAGTGTCCAA 
GACCACTTGA AATTAAATGT TTCTTTTAAC GAAGAAAATG GACGAGAACC GTCATTTGAA 
GATATCGAGA CGCTGACCGC AAAAATGCGT GAAGATTGGA AGATGTTGTC CGTATGTGAC 
ATAGTGATAA ATCACACAGC CAATGAAAGT GATTGGATTA AAGAACACCC TGAAGCAGCA 
TACAATTGCT TGAACTGTCC TTACATGCGA CCAGCCTATC TGTTAGATGA AGCTCTCCAT 
CAGTTTTCCA TGGATATAAG AAAAGGCCTC TATGAGGACC GGGGAATCCC TCTTGACGTT 
TCAACGGAAG ATCATCTAAA CGCAATCCGT TATCACTTTA AACAAAACGT GTTACAACCA 
TTGAAAATTC ATGAGCTGTT TATTGTAGAC GTAACCCAAT ACTTGGAACA GTTTTTAACA 
CAGGCCAGAA AAACCGCACC TCTCAATGAC TGCGAAAGAA ACCTCTTACC GGACTTGAAA 
ATTATTCAAG ATGTTCAGTT TAGGAGATTG AGCAGTTCGG TCGACTTAAA TTTGGCCCTC 
GCCAAGTTCA ACTGCTATCG GCCAGATTGT TACGATGAGG AGTCGAGAGT TAGAAGATGT 
TCCGAGGATT TTAAAATTCA GCTGGATGCC CTCAATCAGG CTGCAATTGA TGAAGTCACT 
GCTGACCTTA GTGCTGCAGT TGAAAACGTT ATTGCTGGCA TTCGGTATTT CAGAGTACAG 
CCAGACGGCC CTCAATATAA GGCCATTACC ATTGATCATC CATTGGTGTA CAGGTACTTT 
ACCGATTATC ATTCACCCAA ATCCCTGGCA GAAGCTGAAG AAACAATGTA CAGTACAAAC 
GGACGGTTTC TCATGGCCCA TAATGGTTGG GTGATGAGCG CTGAGCCATT AAAGAACTTT 
GCCGATGCCG GCACCAAGAT TTACATTAGA AGGGAACTTA TCGCTTGGGG CGACAGCGTT 
AAGCTACGCT TCGGGGACAA GCCGGCCGAT TCTCCATTTT TGTGGAATTA CATGCGGCAG 
TACGTAGAAC AGACGGTTAA AATATTTGAT GGTATTCGGC TGGACAACTG CCACTCAACT 
TCCCTTCCCG TTGCCGAATA TCTTTTGGAC TGCGCCAGAA GAATCCGTCC TGATCTTTAT 
GTGGTGGCCG AGTTGTTCAC CAACTCGGCC TTGACTGACA ACATTTTCGT AAATCGTTTG 
GGCATCACGT CGCTTATTCG AGAAGCGATG TCCGCATGGG ATGCTCATGA ACAGGGACGT 
CTGGTATATC GATTCGGAGG TGTTCCAGTG GGTGCTTTTT ACAAACCTTC AAAAGTACCA 
TTGGTCCCTA TGGTGGCACA TGCACTTTTC CTCGATCAAA CCCATGATAA TCCCAGTCCA 
GTCGAAAAAA GATCTGTTTT CGATTTGCTG CCGAGCGCCG GTTTAGTAAA CATGGCATGT 
TGTGCCACCG GCTCAAATCG AGGCTACGAC GAACTGGTTC CCCACCACAT CCACGTAGTG 
GATGAAACTA GACAATACGC TGAGTGGAGT CATCATGAAA AGCTTCCTTC CAAAACCAGA 
TATGTTAATA AGTGGTCAGG CATTATTGCG GCCAAAAGAG CTCTGCACGA GTTGCACTTT 
AAGCTAGGAA TGGAAGGATT CGACCAGGTA TTTGTGAATC AAGTAGATGG CGATATTGTT 
GAGGTGACCA GGCACCAACC GGGCACCCAC ACCAGTTATG TCCTAGTAGC ATTCACGGCC 
TTTGAACATC CCACCGGTGA CCACCAGAGA GCCATTAAAC CGCTTTCTGT TGAAGGAGTT 
CTGGATGAAA TTGTACTGGA AGCCTACTTA ATTCATGCAG ATTCCACTTC CAGGTTTGGC 
AGATTTGAAA AATTTGAGAA AGACCCCGAT TGGATCAACG GATTAGCCGA ATATAAGCTT 
TCAATTAAAG AGCACATCCA GTTGCGCGAA TCTGAGTTTT TCGAGGAAGC AGCTGACAGT 
AGTTCTAATT TCGTCCGGTT AAACTATAAA AACTTCAAGC CCGGATCAAT TGCAGTGTTG 
AAATTTTCTT TGCCCCAAGG AATCCGAGAT GCGATATCTC TGTTGAAACC CCCACAGGCT 
CCAAATCTAG AGAAAATTGT AAGTGAATTG ACTCTAGCAG ATTTGAACAG AACTCTCTTT 
CGATGTGGCG AAGAAGAAAG GGATGAAGGC AATGGCTTCG ACACCTACAA CATACCCAAC 
TTTGGACCGA CAATTTATTG TGGTTTATTC GGCATTACGT GGGTGCTGCG TCAAATAGCC 
ACAGACAATG ACCTGGGCCA TCCATTGTGT ACCAACTTGC GAAATGGCAA CTGGTTGATT 
GAATACGTTT TCAAACGCCT TAAAGCCGAT GAGGGGACCC GCGCTTTGGG GGAATGGATT 
GAAGACTACA GTAAGCCCCT CCTAAATATG CCCCGTTACT TAGTTCCCTG TTATTTCGAT 
GTCTTCATTG GGCAAATATA CCCTCAGCTA CTAAATGCTG GCTATCAATT GATGTCTAAT 
TTTATCAAGC AGGGATCGCC TTTTGTTCAG TCGTTGGCTT TGGGGTCGTT GCAATTTGGC 
GGTTACCTCA GATCTGCCTG TCTGCCAGAT ATGTCGCCCA ATCTGGCTCC GCCAAAACCT 
CCTAGCAGAA AAAACGAAAA CGGTGAAACA GTCCAGGCCT GCGTTACTTT ATCAGCAGGA 
TTGCCCCACT TCGCCGTGGG TTACATGCGA AATTGGGGTA GAGACACCTT TATTGCTCTG 
CGCGGGCTGT TCATCTTAAC TGGCCGGTTT GAAGAAGCCC GACAACATAT TCTGGGTTAC 
GGGTCGTGTT TGAGGCACGG CCTCATTCCG AATTTACTGG ATGCTGGTAG AAATCCCAGG 
TTTAACTGCC GAGATGCCAT TTGGTGGTGG CTCTATTCGC TCAAATCATA TTGCGAAGAA 
TCCCCTAAGG GCGTTGCTAT TCTTGCCGAT GTTGTATCAC GCATATTTCC TTCGGATGAT 
TCTTCTCCAC AACCGCCTGG TGCTGTGGAT CAACCGCTAT ACAATGTAAT TCAGGAATCG 
TTAACTGTCC ATTTCCAAGG CCTACAATTT CGCGAAAGGA ACGCTGGACG AGAAATTGAT 
GAACACATGA CCGATGCAGG ATTTAACAAC CAAATCGGAA CTCATCCGGA AACTGGATTC 
GTTTTTGGTG GGAATGAATG GAATTGCGGT ACTTGGATGG ACAAAATGGG TTCATCAGAC 
AAGGCTGGCA ATCGGGGCAA ACCGGCGTCG CCTCGTGACG GATCTGCTGT CGAACTCGTA 
GGCTTATCTA AAGCCTGTAT CACTTGGTTA ATGCACTTAA ATGATGAAGG TCAGTACCCT 
TATGATGGGG TTCAGCGAAC ACATAAGAAT GGAACGGTGA CCAAATGGAC CTTCAGAGAA 
TGGTCGGACA AAATTCAACA AAACTTCGAA AAGAAATTCT GGATTTCAGG GAACCCGAAC 
CCAGAAGAAT GTAGGCCTGA TTTAGTGAAT AAGAAGAACA TTTACAAAGA CAGCTTCGGA 
GCGTCTCAGC CATGGACAGA TTTTCAGCTT AGGTGTAATT TTCCCATTGC CATGGTCGCT 
GCTCCCGAGT TGTTTAATCC TCAACGTGCA TGGGCCGCAT TAGAGCAAGT CGAAAAGTAT 
CTCTTGGGGC CACTAGGCAT GAAAACGCTA GACCCCGAAG ACTGGAATTA CCGGGGCGAC 
TATGATAATT CGATTGATTC AGATGATTTA CTTACGGCCC ATGGCTTCAA TTATCATCAA 
GGCCCTGAAT GGGTTTGGCC TGTGGGTTTT TTCCTAAGAG CAAAGTTAAT ATTTGCTGCT 
CAGAATGGAC TGTTAGACGA AACAGTGACC AAAACAAAAC AAATCTTATC AAAGCATTTT 
ATCGAAGTGG AGACGCCACC GTGGCGTGGA TTACCGGAAT TAACCAACAG CGATGGATCC 
TACTGCCCGG ACAGTGCGAG AACCCAAGCT TGGAGCATGT CCTGCATTCT CGAGGTCCTG 
TATGACTTGG AGAAAATCGA ATCCAATAGA AGTGTTGCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019762192.1
CDS	261..5525
Translation	MIVLGACLCLVAILLQICFYLCSFSAQQKIRSKMASLASWLFGRVAGAAPENQTDQNIGQATTSGKNISHASIVTNTVPVGFEASRGTSALPIKEHESASSLEASGETAKSFDDFVHVNIDEENGDSEGGLYEQTSHSDRTLNDPNRQQAAEPSFTNCSTECVDFEVENSSPKCSQIETVENLKIPDIRPGECKRELSAVENRKLNDEPEHPKYIDMASVNILILNDKANLDSELHRIQKNVKLEFRLGPSLLGRNIKLYCNYPKSTNGIVEEFSRDCYTLIRWSRNEGCKNADGSSTFAQITPTIAGSFHYYFVEDDEFTKTNFKNGSGYFIVDPVLKYGANELLPLNCIQSQTVLSKLLGPFSTWENKIRVAKESGYNMVHFTPIQQLGGSHSAYSVQDHLKLNVSFNEENGREPSFEDIETLTAKMREDWKMLSVCDIVINHTANESDWIKEHPEAAYNCLNCPYMRPAYLLDEALHQFSMDIRKGLYEDRGIPLDVSTEDHLNAIRYHFKQNVLQPLKIHELFIVDVTQYLEQFLTQARKTAPLNDCERNLLPDLKIIQDVQFRRLSSSVDLNLALAKFNCYRPDCYDEESRVRRCSEDFKIQLDALNQAAIDEVTADLSAAVENVIAGIRYFRVQPDGPQYKAITIDHPLVYRYFTDYHSPKSLAEAEETMYSTNGRFLMAHNGWVMSAEPLKNFADAGTKIYIRRELIAWGDSVKLRFGDKPADSPFLWNYMRQYVEQTVKIFDGIRLDNCHSTSLPVAEYLLDCARRIRPDLYVVAELFTNSALTDNIFVNRLGITSLIREAMSAWDAHEQGRLVYRFGGVPVGAFYKPSKVPLVPMVAHALFLDQTHDNPSPVEKRSVFDLLPSAGLVNMACCATGSNRGYDELVPHHIHVVDETRQYAEWSHHEKLPSKTRYVNKWSGIIAAKRALHELHFKLGMEGFDQVFVNQVDGDIVEVTRHQPGTHTSYVLVAFTAFEHPTGDHQRAIKPLSVEGVLDEIVLEAYLIHADSTSRFGRFEKFEKDPDWINGLAEYKLSIKEHIQLRESEFFEEAADSSSNFVRLNYKNFKPGSIAVLKFSLPQGIRDAISLLKPPQAPNLEKIVSELTLADLNRTLFRCGEEERDEGNGFDTYNIPNFGPTIYCGLFGITWVLRQIATDNDLGHPLCTNLRNGNWLIEYVFKRLKADEGTRALGEWIEDYSKPLLNMPRYLVPCYFDVFIGQIYPQLLNAGYQLMSNFIKQGSPFVQSLALGSLQFGGYLRSACLPDMSPNLAPPKPPSRKNENGETVQACVTLSAGLPHFAVGYMRNWGRDTFIALRGLFILTGRFEEARQHILGYGSCLRHGLIPNLLDAGRNPRFNCRDAIWWWLYSLKSYCEESPKGVAILADVVSRIFPSDDSSPQPPGAVDQPLYNVIQESLTVHFQGLQFRERNAGREIDEHMTDAGFNNQIGTHPETGFVFGGNEWNCGTWMDKMGSSDKAGNRGKPASPRDGSAVELVGLSKACITWLMHLNDEGQYPYDGVQRTHKNGTVTKWTFREWSDKIQQNFEKKFWISGNPNPEECRPDLVNKKNIYKDSFGASQPWTDFQLRCNFPIAMVAAPELFNPQRAWAALEQVEKYLLGPLGMKTLDPEDWNYRGDYDNSIDSDDLLTAHGFNYHQGPEWVWPVGFFLRAKLIFAAQNGLLDETVTKTKQILSKHFIEVETPPWRGLPELTNSDGSYCPDSARTQAWSMSCILEVLYDLEKIESNRSVAN

Target ORF information:

RefSeq Version	XM_019906633.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019906633.1

ORF Insert Sequence:

ATGATAGTTT TAGGTGCGTG TTTATGTTTG GTTGCTATTC TTCTGCAGAT ATGCTTTTAT 
TTGTGTTCGT TCAGCGCGCA ACAAAAGATC CGATCTAAAA TGGCATCATT GGCATCATGG 
CTCTTCGGCA GAGTTGCAGG TGCCGCACCA GAAAACCAAA CTGACCAGAA CATCGGTCAA 
GCTACAACTT CTGGTAAAAA CATCTCGCAT GCCAGCATAG TCACCAATAC TGTTCCCGTC 
GGTTTTGAGG CTTCTCGTGG TACCTCGGCC CTACCCATCA AAGAACACGA AAGTGCGAGC 
TCTCTTGAAG CATCGGGTGA AACTGCAAAA AGTTTTGACG ATTTCGTGCA CGTAAACATT 
GATGAAGAAA ACGGTGATTC AGAAGGCGGC TTGTATGAAC AGACTTCACA TAGCGATCGA 
ACGCTGAATG ATCCCAATCG GCAGCAAGCA GCCGAACCAA GTTTTACAAA CTGCAGTACC 
GAATGCGTAG ATTTCGAAGT TGAAAACTCT AGTCCTAAAT GTTCACAAAT AGAAACTGTT 
GAAAATTTGA AAATTCCAGA CATTCGACCA GGTGAATGTA AGAGAGAACT AAGCGCCGTT 
GAAAACCGAA AGCTCAACGA CGAGCCGGAA CATCCAAAAT ATATCGACAT GGCATCTGTA 
AATATATTGA TACTGAACGA TAAAGCAAAC TTGGATTCCG AGCTTCACAG AATTCAAAAA 
AATGTTAAAT TGGAGTTCAG ACTCGGCCCT TCTTTGCTGG GCAGGAATAT AAAGCTGTAT 
TGCAACTATC CGAAGTCAAC TAACGGAATT GTGGAAGAAT TCAGCAGAGA TTGTTATACG 
TTAATAAGAT GGTCACGGAA TGAAGGATGC AAAAACGCAG ACGGTAGCTC AACCTTTGCA 
CAGATCACCC CCACGATCGC AGGAAGCTTC CACTACTACT TCGTTGAAGA CGATGAGTTT 
ACTAAGACGA ATTTCAAAAA CGGATCCGGC TATTTTATAG TTGACCCTGT ATTGAAATAT 
GGTGCCAATG AGCTGTTGCC TCTGAACTGT ATACAGAGTC AAACGGTATT GTCAAAACTT 
TTGGGGCCGT TCTCCACATG GGAAAACAAG ATACGGGTTG CCAAAGAATC CGGTTATAAC 
ATGGTGCATT TTACGCCCAT TCAGCAACTA GGAGGATCCC ATTCCGCATA CAGTGTCCAA 
GACCACTTGA AATTAAATGT TTCTTTTAAC GAAGAAAATG GACGAGAACC GTCATTTGAA 
GATATCGAGA CGCTGACCGC AAAAATGCGT GAAGATTGGA AGATGTTGTC CGTATGTGAC 
ATAGTGATAA ATCACACAGC CAATGAAAGT GATTGGATTA AAGAACACCC TGAAGCAGCA 
TACAATTGCT TGAACTGTCC TTACATGCGA CCAGCCTATC TGTTAGATGA AGCTCTCCAT 
CAGTTTTCCA TGGATATAAG AAAAGGCCTC TATGAGGACC GGGGAATCCC TCTTGACGTT 
TCAACGGAAG ATCATCTAAA CGCAATCCGT TATCACTTTA AACAAAACGT GTTACAACCA 
TTGAAAATTC ATGAGCTGTT TATTGTAGAC GTAACCCAAT ACTTGGAACA GTTTTTAACA 
CAGGCCAGAA AAACCGCACC TCTCAATGAC TGCGAAAGAA ACCTCTTACC GGACTTGAAA 
ATTATTCAAG ATGTTCAGTT TAGGAGATTG AGCAGTTCGG TCGACTTAAA TTTGGCCCTC 
GCCAAGTTCA ACTGCTATCG GCCAGATTGT TACGATGAGG AGTCGAGAGT TAGAAGATGT 
TCCGAGGATT TTAAAATTCA GCTGGATGCC CTCAATCAGG CTGCAATTGA TGAAGTCACT 
GCTGACCTTA GTGCTGCAGT TGAAAACGTT ATTGCTGGCA TTCGGTATTT CAGAGTACAG 
CCAGACGGCC CTCAATATAA GGCCATTACC ATTGATCATC CATTGGTGTA CAGGTACTTT 
ACCGATTATC ATTCACCCAA ATCCCTGGCA GAAGCTGAAG AAACAATGTA CAGTACAAAC 
GGACGGTTTC TCATGGCCCA TAATGGTTGG GTGATGAGCG CTGAGCCATT AAAGAACTTT 
GCCGATGCCG GCACCAAGAT TTACATTAGA AGGGAACTTA TCGCTTGGGG CGACAGCGTT 
AAGCTACGCT TCGGGGACAA GCCGGCCGAT TCTCCATTTT TGTGGAATTA CATGCGGCAG 
TACGTAGAAC AGACGGTTAA AATATTTGAT GGTATTCGGC TGGACAACTG CCACTCAACT 
TCCCTTCCCG TTGCCGAATA TCTTTTGGAC TGCGCCAGAA GAATCCGTCC TGATCTTTAT 
GTGGTGGCCG AGTTGTTCAC CAACTCGGCC TTGACTGACA ACATTTTCGT AAATCGTTTG 
GGCATCACGT CGCTTATTCG AGAAGCGATG TCCGCATGGG ATGCTCATGA ACAGGGACGT 
CTGGTATATC GATTCGGAGG TGTTCCAGTG GGTGCTTTTT ACAAACCTTC AAAAGTACCA 
TTGGTCCCTA TGGTGGCACA TGCACTTTTC CTCGATCAAA CCCATGATAA TCCCAGTCCA 
GTCGAAAAAA GATCTGTTTT CGATTTGCTG CCGAGCGCCG GTTTAGTAAA CATGGCATGT 
TGTGCCACCG GCTCAAATCG AGGCTACGAC GAACTGGTTC CCCACCACAT CCACGTAGTG 
GATGAAACTA GACAATACGC TGAGTGGAGT CATCATGAAA AGCTTCCTTC CAAAACCAGA 
TATGTTAATA AGTGGTCAGG CATTATTGCG GCCAAAAGAG CTCTGCACGA GTTGCACTTT 
AAGCTAGGAA TGGAAGGATT CGACCAGGTA TTTGTGAATC AAGTAGATGG CGATATTGTT 
GAGGTGACCA GGCACCAACC GGGCACCCAC ACCAGTTATG TCCTAGTAGC ATTCACGGCC 
TTTGAACATC CCACCGGTGA CCACCAGAGA GCCATTAAAC CGCTTTCTGT TGAAGGAGTT 
CTGGATGAAA TTGTACTGGA AGCCTACTTA ATTCATGCAG ATTCCACTTC CAGGTTTGGC 
AGATTTGAAA AATTTGAGAA AGACCCCGAT TGGATCAACG GATTAGCCGA ATATAAGCTT 
TCAATTAAAG AGCACATCCA GTTGCGCGAA TCTGAGTTTT TCGAGGAAGC AGCTGACAGT 
AGTTCTAATT TCGTCCGGTT AAACTATAAA AACTTCAAGC CCGGATCAAT TGCAGTGTTG 
AAATTTTCTT TGCCCCAAGG AATCCGAGAT GCGATATCTC TGTTGAAACC CCCACAGGCT 
CCAAATCTAG AGAAAATTGT AAGTGAATTG ACTCTAGCAG ATTTGAACAG AACTCTCTTT 
CGATGTGGCG AAGAAGAAAG GGATGAAGGC AATGGCTTCG ACACCTACAA CATACCCAAC 
TTTGGACCGA CAATTTATTG TGGTTTATTC GGCATTACGT GGGTGCTGCG TCAAATAGCC 
ACAGACAATG ACCTGGGCCA TCCATTGTGT ACCAACTTGC GAAATGGCAA CTGGTTGATT 
GAATACGTTT TCAAACGCCT TAAAGCCGAT GAGGGGACCC GCGCTTTGGG GGAATGGATT 
GAAGACTACA GTAAGCCCCT CCTAAATATG CCCCGTTACT TAGTTCCCTG TTATTTCGAT 
GTCTTCATTG GGCAAATATA CCCTCAGCTA CTAAATGCTG GCTATCAATT GATGTCTAAT 
TTTATCAAGC AGGGATCGCC TTTTGTTCAG TCGTTGGCTT TGGGGTCGTT GCAATTTGGC 
GGTTACCTCA GATCTGCCTG TCTGCCAGAT ATGTCGCCCA ATCTGGCTCC GCCAAAACCT 
CCTAGCAGAA AAAACGAAAA CGGTGAAACA GTCCAGGCCT GCGTTACTTT ATCAGCAGGA 
TTGCCCCACT TCGCCGTGGG TTACATGCGA AATTGGGGTA GAGACACCTT TATTGCTCTG 
CGCGGGCTGT TCATCTTAAC TGGCCGGTTT GAAGAAGCCC GACAACATAT TCTGGGTTAC 
GGGTCGTGTT TGAGGCACGG CCTCATTCCG AATTTACTGG ATGCTGGTAG AAATCCCAGG 
TTTAACTGCC GAGATGCCAT TTGGTGGTGG CTCTATTCGC TCAAATCATA TTGCGAAGAA 
TCCCCTAAGG GCGTTGCTAT TCTTGCCGAT GTTGTATCAC GCATATTTCC TTCGGATGAT 
TCTTCTCCAC AACCGCCTGG TGCTGTGGAT CAACCGCTAT ACAATGTAAT TCAGGAATCG 
TTAACTGTCC ATTTCCAAGG CCTACAATTT CGCGAAAGGA ACGCTGGACG AGAAATTGAT 
GAACACATGA CCGATGCAGG ATTTAACAAC CAAATCGGAA CTCATCCGGA AACTGGATTC 
GTTTTTGGTG GGAATGAATG GAATTGCGGT ACTTGGATGG ACAAAATGGG TTCATCAGAC 
AAGGCTGGCA ATCGGGGCAA ACCGGCGTCG CCTCGTGACG GATCTGCTGT CGAACTCGTA 
GGCTTATCTA AAGCCTGTAT CACTTGGTTA ATGCACTTAA ATGATGAAGG TCAGTACCCT 
TATGATGGGG TTCAGCGAAC ACATAAGAAT GGAACGGTGA CCAAATGGAC CTTCAGAGAA 
TGGTCGGACA AAATTCAACA AAACTTCGAA AAGAAATTCT GGATTTCAGG GAACCCGAAC 
CCAGAAGAAT GTAGGCCTGA TTTAGTGAAT AAGAAGAACA TTTACAAAGA CAGCTTCGGA 
GCGTCTCAGC CATGGACAGA TTTTCAGCTT AGGTGTAATT TTCCCATTGC CATGGTCGCT 
GCTCCCGAGT TGTTTAATCC TCAACGTGCA TGGGCCGCAT TAGAGCAAGT CGAAAAGTAT 
CTCTTGGGGC CACTAGGCAT GAAAACGCTA GACCCCGAAG ACTGGAATTA CCGGGGCGAC 
TATGATAATT CGATTGATTC AGATGATTTA CTTACGGCCC ATGGCTTCAA TTATCATCAA 
GGCCCTGAAT GGGTTTGGCC TGTGGGTTTT TTCCTAAGAG CAAAGTTAAT ATTTGCTGCT 
CAGAATGGAC TGTTAGACGA AACAGTGACC AAAACAAAAC AAATCTTATC AAAGCATTTT 
ATCGAAGTGG AGACGCCACC GTGGCGTGGA TTACCGGAAT TAACCAACAG CGATGGATCC 
TACTGCCCGG ACAGTGCGAG AACCCAAGCT TGGAGCATGT CCTGCATTCT CGAGGTCCTG 
TATGACTTGG AGAAAATCGA ATCCAATAGA AGTGTTGCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109539064 ( XM_019906634.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019762193.1 Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X3, mRNA.

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CloneID	ODe42979
Clone ID Related Accession (Same CDS sequence)	XM_019906634.1
Accession Version	XM_019906634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	glycogen debranching enzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGAGT CTACTGTTCC GGTAACAGCG ATTGGGGGAA TCATTTTGTT GATAGTCTGT 
TCGAAAAGGA TCCGATCTAA AATGGCATCA TTGGCATCAT GGCTCTTCGG CAGAGTTGCA 
GGTGCCGCAC CAGAAAACCA AACTGACCAG AACATCGGTC AAGCTACAAC TTCTGGTAAA 
AACATCTCGC ATGCCAGCAT AGTCACCAAT ACTGTTCCCG TCGGTTTTGA GGCTTCTCGT 
GGTACCTCGG CCCTACCCAT CAAAGAACAC GAAAGTGCGA GCTCTCTTGA AGCATCGGGT 
GAAACTGCAA AAAGTTTTGA CGATTTCGTG CACGTAAACA TTGATGAAGA AAACGGTGAT 
TCAGAAGGCG GCTTGTATGA ACAGACTTCA CATAGCGATC GAACGCTGAA TGATCCCAAT 
CGGCAGCAAG CAGCCGAACC AAGTTTTACA AACTGCAGTA CCGAATGCGT AGATTTCGAA 
GTTGAAAACT CTAGTCCTAA ATGTTCACAA ATAGAAACTG TTGAAAATTT GAAAATTCCA 
GACATTCGAC CAGGTGAATG TAAGAGAGAA CTAAGCGCCG TTGAAAACCG AAAGCTCAAC 
GACGAGCCGG AACATCCAAA ATATATCGAC ATGGCATCTG TAAATATATT GATACTGAAC 
GATAAAGCAA ACTTGGATTC CGAGCTTCAC AGAATTCAAA AAAATGTTAA ATTGGAGTTC 
AGACTCGGCC CTTCTTTGCT GGGCAGGAAT ATAAAGCTGT ATTGCAACTA TCCGAAGTCA 
ACTAACGGAA TTGTGGAAGA ATTCAGCAGA GATTGTTATA CGTTAATAAG ATGGTCACGG 
AATGAAGGAT GCAAAAACGC AGACGGTAGC TCAACCTTTG CACAGATCAC CCCCACGATC 
GCAGGAAGCT TCCACTACTA CTTCGTTGAA GACGATGAGT TTACTAAGAC GAATTTCAAA 
AACGGATCCG GCTATTTTAT AGTTGACCCT GTATTGAAAT ATGGTGCCAA TGAGCTGTTG 
CCTCTGAACT GTATACAGAG TCAAACGGTA TTGTCAAAAC TTTTGGGGCC GTTCTCCACA 
TGGGAAAACA AGATACGGGT TGCCAAAGAA TCCGGTTATA ACATGGTGCA TTTTACGCCC 
ATTCAGCAAC TAGGAGGATC CCATTCCGCA TACAGTGTCC AAGACCACTT GAAATTAAAT 
GTTTCTTTTA ACGAAGAAAA TGGACGAGAA CCGTCATTTG AAGATATCGA GACGCTGACC 
GCAAAAATGC GTGAAGATTG GAAGATGTTG TCCGTATGTG ACATAGTGAT AAATCACACA 
GCCAATGAAA GTGATTGGAT TAAAGAACAC CCTGAAGCAG CATACAATTG CTTGAACTGT 
CCTTACATGC GACCAGCCTA TCTGTTAGAT GAAGCTCTCC ATCAGTTTTC CATGGATATA 
AGAAAAGGCC TCTATGAGGA CCGGGGAATC CCTCTTGACG TTTCAACGGA AGATCATCTA 
AACGCAATCC GTTATCACTT TAAACAAAAC GTGTTACAAC CATTGAAAAT TCATGAGCTG 
TTTATTGTAG ACGTAACCCA ATACTTGGAA CAGTTTTTAA CACAGGCCAG AAAAACCGCA 
CCTCTCAATG ACTGCGAAAG AAACCTCTTA CCGGACTTGA AAATTATTCA AGATGTTCAG 
TTTAGGAGAT TGAGCAGTTC GGTCGACTTA AATTTGGCCC TCGCCAAGTT CAACTGCTAT 
CGGCCAGATT GTTACGATGA GGAGTCGAGA GTTAGAAGAT GTTCCGAGGA TTTTAAAATT 
CAGCTGGATG CCCTCAATCA GGCTGCAATT GATGAAGTCA CTGCTGACCT TAGTGCTGCA 
GTTGAAAACG TTATTGCTGG CATTCGGTAT TTCAGAGTAC AGCCAGACGG CCCTCAATAT 
AAGGCCATTA CCATTGATCA TCCATTGGTG TACAGGTACT TTACCGATTA TCATTCACCC 
AAATCCCTGG CAGAAGCTGA AGAAACAATG TACAGTACAA ACGGACGGTT TCTCATGGCC 
CATAATGGTT GGGTGATGAG CGCTGAGCCA TTAAAGAACT TTGCCGATGC CGGCACCAAG 
ATTTACATTA GAAGGGAACT TATCGCTTGG GGCGACAGCG TTAAGCTACG CTTCGGGGAC 
AAGCCGGCCG ATTCTCCATT TTTGTGGAAT TACATGCGGC AGTACGTAGA ACAGACGGTT 
AAAATATTTG ATGGTATTCG GCTGGACAAC TGCCACTCAA CTTCCCTTCC CGTTGCCGAA 
TATCTTTTGG ACTGCGCCAG AAGAATCCGT CCTGATCTTT ATGTGGTGGC CGAGTTGTTC 
ACCAACTCGG CCTTGACTGA CAACATTTTC GTAAATCGTT TGGGCATCAC GTCGCTTATT 
CGAGAAGCGA TGTCCGCATG GGATGCTCAT GAACAGGGAC GTCTGGTATA TCGATTCGGA 
GGTGTTCCAG TGGGTGCTTT TTACAAACCT TCAAAAGTAC CATTGGTCCC TATGGTGGCA 
CATGCACTTT TCCTCGATCA AACCCATGAT AATCCCAGTC CAGTCGAAAA AAGATCTGTT 
TTCGATTTGC TGCCGAGCGC CGGTTTAGTA AACATGGCAT GTTGTGCCAC CGGCTCAAAT 
CGAGGCTACG ACGAACTGGT TCCCCACCAC ATCCACGTAG TGGATGAAAC TAGACAATAC 
GCTGAGTGGA GTCATCATGA AAAGCTTCCT TCCAAAACCA GATATGTTAA TAAGTGGTCA 
GGCATTATTG CGGCCAAAAG AGCTCTGCAC GAGTTGCACT TTAAGCTAGG AATGGAAGGA 
TTCGACCAGG TATTTGTGAA TCAAGTAGAT GGCGATATTG TTGAGGTGAC CAGGCACCAA 
CCGGGCACCC ACACCAGTTA TGTCCTAGTA GCATTCACGG CCTTTGAACA TCCCACCGGT 
GACCACCAGA GAGCCATTAA ACCGCTTTCT GTTGAAGGAG TTCTGGATGA AATTGTACTG 
GAAGCCTACT TAATTCATGC AGATTCCACT TCCAGGTTTG GCAGATTTGA AAAATTTGAG 
AAAGACCCCG ATTGGATCAA CGGATTAGCC GAATATAAGC TTTCAATTAA AGAGCACATC 
CAGTTGCGCG AATCTGAGTT TTTCGAGGAA GCAGCTGACA GTAGTTCTAA TTTCGTCCGG 
TTAAACTATA AAAACTTCAA GCCCGGATCA ATTGCAGTGT TGAAATTTTC TTTGCCCCAA 
GGAATCCGAG ATGCGATATC TCTGTTGAAA CCCCCACAGG CTCCAAATCT AGAGAAAATT 
GTAAGTGAAT TGACTCTAGC AGATTTGAAC AGAACTCTCT TTCGATGTGG CGAAGAAGAA 
AGGGATGAAG GCAATGGCTT CGACACCTAC AACATACCCA ACTTTGGACC GACAATTTAT 
TGTGGTTTAT TCGGCATTAC GTGGGTGCTG CGTCAAATAG CCACAGACAA TGACCTGGGC 
CATCCATTGT GTACCAACTT GCGAAATGGC AACTGGTTGA TTGAATACGT TTTCAAACGC 
CTTAAAGCCG ATGAGGGGAC CCGCGCTTTG GGGGAATGGA TTGAAGACTA CAGTAAGCCC 
CTCCTAAATA TGCCCCGTTA CTTAGTTCCC TGTTATTTCG ATGTCTTCAT TGGGCAAATA 
TACCCTCAGC TACTAAATGC TGGCTATCAA TTGATGTCTA ATTTTATCAA GCAGGGATCG 
CCTTTTGTTC AGTCGTTGGC TTTGGGGTCG TTGCAATTTG GCGGTTACCT CAGATCTGCC 
TGTCTGCCAG ATATGTCGCC CAATCTGGCT CCGCCAAAAC CTCCTAGCAG AAAAAACGAA 
AACGGTGAAA CAGTCCAGGC CTGCGTTACT TTATCAGCAG GATTGCCCCA CTTCGCCGTG 
GGTTACATGC GAAATTGGGG TAGAGACACC TTTATTGCTC TGCGCGGGCT GTTCATCTTA 
ACTGGCCGGT TTGAAGAAGC CCGACAACAT ATTCTGGGTT ACGGGTCGTG TTTGAGGCAC 
GGCCTCATTC CGAATTTACT GGATGCTGGT AGAAATCCCA GGTTTAACTG CCGAGATGCC 
ATTTGGTGGT GGCTCTATTC GCTCAAATCA TATTGCGAAG AATCCCCTAA GGGCGTTGCT 
ATTCTTGCCG ATGTTGTATC ACGCATATTT CCTTCGGATG ATTCTTCTCC ACAACCGCCT 
GGTGCTGTGG ATCAACCGCT ATACAATGTA ATTCAGGAAT CGTTAACTGT CCATTTCCAA 
GGCCTACAAT TTCGCGAAAG GAACGCTGGA CGAGAAATTG ATGAACACAT GACCGATGCA 
GGATTTAACA ACCAAATCGG AACTCATCCG GAAACTGGAT TCGTTTTTGG TGGGAATGAA 
TGGAATTGCG GTACTTGGAT GGACAAAATG GGTTCATCAG ACAAGGCTGG CAATCGGGGC 
AAACCGGCGT CGCCTCGTGA CGGATCTGCT GTCGAACTCG TAGGCTTATC TAAAGCCTGT 
ATCACTTGGT TAATGCACTT AAATGATGAA GGTCAGTACC CTTATGATGG GGTTCAGCGA 
ACACATAAGA ATGGAACGGT GACCAAATGG ACCTTCAGAG AATGGTCGGA CAAAATTCAA 
CAAAACTTCG AAAAGAAATT CTGGATTTCA GGGAACCCGA ACCCAGAAGA ATGTAGGCCT 
GATTTAGTGA ATAAGAAGAA CATTTACAAA GACAGCTTCG GAGCGTCTCA GCCATGGACA 
GATTTTCAGC TTAGGTGTAA TTTTCCCATT GCCATGGTCG CTGCTCCCGA GTTGTTTAAT 
CCTCAACGTG CATGGGCCGC ATTAGAGCAA GTCGAAAAGT ATCTCTTGGG GCCACTAGGC 
ATGAAAACGC TAGACCCCGA AGACTGGAAT TACCGGGGCG ACTATGATAA TTCGATTGAT 
TCAGATGATT TACTTACGGC CCATGGCTTC AATTATCATC AAGGCCCTGA ATGGGTTTGG 
CCTGTGGGTT TTTTCCTAAG AGCAAAGTTA ATATTTGCTG CTCAGAATGG ACTGTTAGAC 
GAAACAGTGA CCAAAACAAA ACAAATCTTA TCAAAGCATT TTATCGAAGT GGAGACGCCA 
CCGTGGCGTG GATTACCGGA ATTAACCAAC AGCGATGGAT CCTACTGCCC GGACAGTGCG 
AGAACCCAAG CTTGGAGCAT GTCCTGCATT CTCGAGGTCC TGTATGACTT GGAGAAAATC 
GAATCCAATA GAAGTGTTGC AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019762193.1
CDS	261..5507
Translation	MNESTVPVTAIGGIILLIVCSKRIRSKMASLASWLFGRVAGAAPENQTDQNIGQATTSGKNISHASIVTNTVPVGFEASRGTSALPIKEHESASSLEASGETAKSFDDFVHVNIDEENGDSEGGLYEQTSHSDRTLNDPNRQQAAEPSFTNCSTECVDFEVENSSPKCSQIETVENLKIPDIRPGECKRELSAVENRKLNDEPEHPKYIDMASVNILILNDKANLDSELHRIQKNVKLEFRLGPSLLGRNIKLYCNYPKSTNGIVEEFSRDCYTLIRWSRNEGCKNADGSSTFAQITPTIAGSFHYYFVEDDEFTKTNFKNGSGYFIVDPVLKYGANELLPLNCIQSQTVLSKLLGPFSTWENKIRVAKESGYNMVHFTPIQQLGGSHSAYSVQDHLKLNVSFNEENGREPSFEDIETLTAKMREDWKMLSVCDIVINHTANESDWIKEHPEAAYNCLNCPYMRPAYLLDEALHQFSMDIRKGLYEDRGIPLDVSTEDHLNAIRYHFKQNVLQPLKIHELFIVDVTQYLEQFLTQARKTAPLNDCERNLLPDLKIIQDVQFRRLSSSVDLNLALAKFNCYRPDCYDEESRVRRCSEDFKIQLDALNQAAIDEVTADLSAAVENVIAGIRYFRVQPDGPQYKAITIDHPLVYRYFTDYHSPKSLAEAEETMYSTNGRFLMAHNGWVMSAEPLKNFADAGTKIYIRRELIAWGDSVKLRFGDKPADSPFLWNYMRQYVEQTVKIFDGIRLDNCHSTSLPVAEYLLDCARRIRPDLYVVAELFTNSALTDNIFVNRLGITSLIREAMSAWDAHEQGRLVYRFGGVPVGAFYKPSKVPLVPMVAHALFLDQTHDNPSPVEKRSVFDLLPSAGLVNMACCATGSNRGYDELVPHHIHVVDETRQYAEWSHHEKLPSKTRYVNKWSGIIAAKRALHELHFKLGMEGFDQVFVNQVDGDIVEVTRHQPGTHTSYVLVAFTAFEHPTGDHQRAIKPLSVEGVLDEIVLEAYLIHADSTSRFGRFEKFEKDPDWINGLAEYKLSIKEHIQLRESEFFEEAADSSSNFVRLNYKNFKPGSIAVLKFSLPQGIRDAISLLKPPQAPNLEKIVSELTLADLNRTLFRCGEEERDEGNGFDTYNIPNFGPTIYCGLFGITWVLRQIATDNDLGHPLCTNLRNGNWLIEYVFKRLKADEGTRALGEWIEDYSKPLLNMPRYLVPCYFDVFIGQIYPQLLNAGYQLMSNFIKQGSPFVQSLALGSLQFGGYLRSACLPDMSPNLAPPKPPSRKNENGETVQACVTLSAGLPHFAVGYMRNWGRDTFIALRGLFILTGRFEEARQHILGYGSCLRHGLIPNLLDAGRNPRFNCRDAIWWWLYSLKSYCEESPKGVAILADVVSRIFPSDDSSPQPPGAVDQPLYNVIQESLTVHFQGLQFRERNAGREIDEHMTDAGFNNQIGTHPETGFVFGGNEWNCGTWMDKMGSSDKAGNRGKPASPRDGSAVELVGLSKACITWLMHLNDEGQYPYDGVQRTHKNGTVTKWTFREWSDKIQQNFEKKFWISGNPNPEECRPDLVNKKNIYKDSFGASQPWTDFQLRCNFPIAMVAAPELFNPQRAWAALEQVEKYLLGPLGMKTLDPEDWNYRGDYDNSIDSDDLLTAHGFNYHQGPEWVWPVGFFLRAKLIFAAQNGLLDETVTKTKQILSKHFIEVETPPWRGLPELTNSDGSYCPDSARTQAWSMSCILEVLYDLEKIESNRSVAN

Target ORF information:

RefSeq Version	XM_019906634.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019906634.1

ORF Insert Sequence:

ATGAACGAGT CTACTGTTCC GGTAACAGCG ATTGGGGGAA TCATTTTGTT GATAGTCTGT 
TCGAAAAGGA TCCGATCTAA AATGGCATCA TTGGCATCAT GGCTCTTCGG CAGAGTTGCA 
GGTGCCGCAC CAGAAAACCA AACTGACCAG AACATCGGTC AAGCTACAAC TTCTGGTAAA 
AACATCTCGC ATGCCAGCAT AGTCACCAAT ACTGTTCCCG TCGGTTTTGA GGCTTCTCGT 
GGTACCTCGG CCCTACCCAT CAAAGAACAC GAAAGTGCGA GCTCTCTTGA AGCATCGGGT 
GAAACTGCAA AAAGTTTTGA CGATTTCGTG CACGTAAACA TTGATGAAGA AAACGGTGAT 
TCAGAAGGCG GCTTGTATGA ACAGACTTCA CATAGCGATC GAACGCTGAA TGATCCCAAT 
CGGCAGCAAG CAGCCGAACC AAGTTTTACA AACTGCAGTA CCGAATGCGT AGATTTCGAA 
GTTGAAAACT CTAGTCCTAA ATGTTCACAA ATAGAAACTG TTGAAAATTT GAAAATTCCA 
GACATTCGAC CAGGTGAATG TAAGAGAGAA CTAAGCGCCG TTGAAAACCG AAAGCTCAAC 
GACGAGCCGG AACATCCAAA ATATATCGAC ATGGCATCTG TAAATATATT GATACTGAAC 
GATAAAGCAA ACTTGGATTC CGAGCTTCAC AGAATTCAAA AAAATGTTAA ATTGGAGTTC 
AGACTCGGCC CTTCTTTGCT GGGCAGGAAT ATAAAGCTGT ATTGCAACTA TCCGAAGTCA 
ACTAACGGAA TTGTGGAAGA ATTCAGCAGA GATTGTTATA CGTTAATAAG ATGGTCACGG 
AATGAAGGAT GCAAAAACGC AGACGGTAGC TCAACCTTTG CACAGATCAC CCCCACGATC 
GCAGGAAGCT TCCACTACTA CTTCGTTGAA GACGATGAGT TTACTAAGAC GAATTTCAAA 
AACGGATCCG GCTATTTTAT AGTTGACCCT GTATTGAAAT ATGGTGCCAA TGAGCTGTTG 
CCTCTGAACT GTATACAGAG TCAAACGGTA TTGTCAAAAC TTTTGGGGCC GTTCTCCACA 
TGGGAAAACA AGATACGGGT TGCCAAAGAA TCCGGTTATA ACATGGTGCA TTTTACGCCC 
ATTCAGCAAC TAGGAGGATC CCATTCCGCA TACAGTGTCC AAGACCACTT GAAATTAAAT 
GTTTCTTTTA ACGAAGAAAA TGGACGAGAA CCGTCATTTG AAGATATCGA GACGCTGACC 
GCAAAAATGC GTGAAGATTG GAAGATGTTG TCCGTATGTG ACATAGTGAT AAATCACACA 
GCCAATGAAA GTGATTGGAT TAAAGAACAC CCTGAAGCAG CATACAATTG CTTGAACTGT 
CCTTACATGC GACCAGCCTA TCTGTTAGAT GAAGCTCTCC ATCAGTTTTC CATGGATATA 
AGAAAAGGCC TCTATGAGGA CCGGGGAATC CCTCTTGACG TTTCAACGGA AGATCATCTA 
AACGCAATCC GTTATCACTT TAAACAAAAC GTGTTACAAC CATTGAAAAT TCATGAGCTG 
TTTATTGTAG ACGTAACCCA ATACTTGGAA CAGTTTTTAA CACAGGCCAG AAAAACCGCA 
CCTCTCAATG ACTGCGAAAG AAACCTCTTA CCGGACTTGA AAATTATTCA AGATGTTCAG 
TTTAGGAGAT TGAGCAGTTC GGTCGACTTA AATTTGGCCC TCGCCAAGTT CAACTGCTAT 
CGGCCAGATT GTTACGATGA GGAGTCGAGA GTTAGAAGAT GTTCCGAGGA TTTTAAAATT 
CAGCTGGATG CCCTCAATCA GGCTGCAATT GATGAAGTCA CTGCTGACCT TAGTGCTGCA 
GTTGAAAACG TTATTGCTGG CATTCGGTAT TTCAGAGTAC AGCCAGACGG CCCTCAATAT 
AAGGCCATTA CCATTGATCA TCCATTGGTG TACAGGTACT TTACCGATTA TCATTCACCC 
AAATCCCTGG CAGAAGCTGA AGAAACAATG TACAGTACAA ACGGACGGTT TCTCATGGCC 
CATAATGGTT GGGTGATGAG CGCTGAGCCA TTAAAGAACT TTGCCGATGC CGGCACCAAG 
ATTTACATTA GAAGGGAACT TATCGCTTGG GGCGACAGCG TTAAGCTACG CTTCGGGGAC 
AAGCCGGCCG ATTCTCCATT TTTGTGGAAT TACATGCGGC AGTACGTAGA ACAGACGGTT 
AAAATATTTG ATGGTATTCG GCTGGACAAC TGCCACTCAA CTTCCCTTCC CGTTGCCGAA 
TATCTTTTGG ACTGCGCCAG AAGAATCCGT CCTGATCTTT ATGTGGTGGC CGAGTTGTTC 
ACCAACTCGG CCTTGACTGA CAACATTTTC GTAAATCGTT TGGGCATCAC GTCGCTTATT 
CGAGAAGCGA TGTCCGCATG GGATGCTCAT GAACAGGGAC GTCTGGTATA TCGATTCGGA 
GGTGTTCCAG TGGGTGCTTT TTACAAACCT TCAAAAGTAC CATTGGTCCC TATGGTGGCA 
CATGCACTTT TCCTCGATCA AACCCATGAT AATCCCAGTC CAGTCGAAAA AAGATCTGTT 
TTCGATTTGC TGCCGAGCGC CGGTTTAGTA AACATGGCAT GTTGTGCCAC CGGCTCAAAT 
CGAGGCTACG ACGAACTGGT TCCCCACCAC ATCCACGTAG TGGATGAAAC TAGACAATAC 
GCTGAGTGGA GTCATCATGA AAAGCTTCCT TCCAAAACCA GATATGTTAA TAAGTGGTCA 
GGCATTATTG CGGCCAAAAG AGCTCTGCAC GAGTTGCACT TTAAGCTAGG AATGGAAGGA 
TTCGACCAGG TATTTGTGAA TCAAGTAGAT GGCGATATTG TTGAGGTGAC CAGGCACCAA 
CCGGGCACCC ACACCAGTTA TGTCCTAGTA GCATTCACGG CCTTTGAACA TCCCACCGGT 
GACCACCAGA GAGCCATTAA ACCGCTTTCT GTTGAAGGAG TTCTGGATGA AATTGTACTG 
GAAGCCTACT TAATTCATGC AGATTCCACT TCCAGGTTTG GCAGATTTGA AAAATTTGAG 
AAAGACCCCG ATTGGATCAA CGGATTAGCC GAATATAAGC TTTCAATTAA AGAGCACATC 
CAGTTGCGCG AATCTGAGTT TTTCGAGGAA GCAGCTGACA GTAGTTCTAA TTTCGTCCGG 
TTAAACTATA AAAACTTCAA GCCCGGATCA ATTGCAGTGT TGAAATTTTC TTTGCCCCAA 
GGAATCCGAG ATGCGATATC TCTGTTGAAA CCCCCACAGG CTCCAAATCT AGAGAAAATT 
GTAAGTGAAT TGACTCTAGC AGATTTGAAC AGAACTCTCT TTCGATGTGG CGAAGAAGAA 
AGGGATGAAG GCAATGGCTT CGACACCTAC AACATACCCA ACTTTGGACC GACAATTTAT 
TGTGGTTTAT TCGGCATTAC GTGGGTGCTG CGTCAAATAG CCACAGACAA TGACCTGGGC 
CATCCATTGT GTACCAACTT GCGAAATGGC AACTGGTTGA TTGAATACGT TTTCAAACGC 
CTTAAAGCCG ATGAGGGGAC CCGCGCTTTG GGGGAATGGA TTGAAGACTA CAGTAAGCCC 
CTCCTAAATA TGCCCCGTTA CTTAGTTCCC TGTTATTTCG ATGTCTTCAT TGGGCAAATA 
TACCCTCAGC TACTAAATGC TGGCTATCAA TTGATGTCTA ATTTTATCAA GCAGGGATCG 
CCTTTTGTTC AGTCGTTGGC TTTGGGGTCG TTGCAATTTG GCGGTTACCT CAGATCTGCC 
TGTCTGCCAG ATATGTCGCC CAATCTGGCT CCGCCAAAAC CTCCTAGCAG AAAAAACGAA 
AACGGTGAAA CAGTCCAGGC CTGCGTTACT TTATCAGCAG GATTGCCCCA CTTCGCCGTG 
GGTTACATGC GAAATTGGGG TAGAGACACC TTTATTGCTC TGCGCGGGCT GTTCATCTTA 
ACTGGCCGGT TTGAAGAAGC CCGACAACAT ATTCTGGGTT ACGGGTCGTG TTTGAGGCAC 
GGCCTCATTC CGAATTTACT GGATGCTGGT AGAAATCCCA GGTTTAACTG CCGAGATGCC 
ATTTGGTGGT GGCTCTATTC GCTCAAATCA TATTGCGAAG AATCCCCTAA GGGCGTTGCT 
ATTCTTGCCG ATGTTGTATC ACGCATATTT CCTTCGGATG ATTCTTCTCC ACAACCGCCT 
GGTGCTGTGG ATCAACCGCT ATACAATGTA ATTCAGGAAT CGTTAACTGT CCATTTCCAA 
GGCCTACAAT TTCGCGAAAG GAACGCTGGA CGAGAAATTG ATGAACACAT GACCGATGCA 
GGATTTAACA ACCAAATCGG AACTCATCCG GAAACTGGAT TCGTTTTTGG TGGGAATGAA 
TGGAATTGCG GTACTTGGAT GGACAAAATG GGTTCATCAG ACAAGGCTGG CAATCGGGGC 
AAACCGGCGT CGCCTCGTGA CGGATCTGCT GTCGAACTCG TAGGCTTATC TAAAGCCTGT 
ATCACTTGGT TAATGCACTT AAATGATGAA GGTCAGTACC CTTATGATGG GGTTCAGCGA 
ACACATAAGA ATGGAACGGT GACCAAATGG ACCTTCAGAG AATGGTCGGA CAAAATTCAA 
CAAAACTTCG AAAAGAAATT CTGGATTTCA GGGAACCCGA ACCCAGAAGA ATGTAGGCCT 
GATTTAGTGA ATAAGAAGAA CATTTACAAA GACAGCTTCG GAGCGTCTCA GCCATGGACA 
GATTTTCAGC TTAGGTGTAA TTTTCCCATT GCCATGGTCG CTGCTCCCGA GTTGTTTAAT 
CCTCAACGTG CATGGGCCGC ATTAGAGCAA GTCGAAAAGT ATCTCTTGGG GCCACTAGGC 
ATGAAAACGC TAGACCCCGA AGACTGGAAT TACCGGGGCG ACTATGATAA TTCGATTGAT 
TCAGATGATT TACTTACGGC CCATGGCTTC AATTATCATC AAGGCCCTGA ATGGGTTTGG 
CCTGTGGGTT TTTTCCTAAG AGCAAAGTTA ATATTTGCTG CTCAGAATGG ACTGTTAGAC 
GAAACAGTGA CCAAAACAAA ACAAATCTTA TCAAAGCATT TTATCGAAGT GGAGACGCCA 
CCGTGGCGTG GATTACCGGA ATTAACCAAC AGCGATGGAT CCTACTGCCC GGACAGTGCG 
AGAACCCAAG CTTGGAGCAT GTCCTGCATT CTCGAGGTCC TGTATGACTT GGAGAAAATC 
GAATCCAATA GAAGTGTTGC AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109539064 ( XM_019906636.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019762195.1 Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X5, mRNA.

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CloneID	ODe42980
Clone ID Related Accession (Same CDS sequence)	XM_019906636.1 , XM_019906635.1
Accession Version	XM_019906636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	glycogen debranching enzyme isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCGAT CTAAAATGGC ATCATTGGCA TCATGGCTCT TCGGCAGAGT TGCAGGTGCC 
GCACCAGAAA ACCAAACTGA CCAGAACATC GGTCAAGCTA CAACTTCTGG TAAAAACATC 
TCGCATGCCA GCATAGTCAC CAATACTGTT CCCGTCGGTT TTGAGGCTTC TCGTGGTACC 
TCGGCCCTAC CCATCAAAGA ACACGAAAGT GCGAGCTCTC TTGAAGCATC GGGTGAAACT 
GCAAAAAGTT TTGACGATTT CGTGCACGTA AACATTGATG AAGAAAACGG TGATTCAGAA 
GGCGGCTTGT ATGAACAGAC TTCACATAGC GATCGAACGC TGAATGATCC CAATCGGCAG 
CAAGCAGCCG AACCAAGTTT TACAAACTGC AGTACCGAAT GCGTAGATTT CGAAGTTGAA 
AACTCTAGTC CTAAATGTTC ACAAATAGAA ACTGTTGAAA ATTTGAAAAT TCCAGACATT 
CGACCAGGTG AATGTAAGAG AGAACTAAGC GCCGTTGAAA ACCGAAAGCT CAACGACGAG 
CCGGAACATC CAAAATATAT CGACATGGCA TCTGTAAATA TATTGATACT GAACGATAAA 
GCAAACTTGG ATTCCGAGCT TCACAGAATT CAAAAAAATG TTAAATTGGA GTTCAGACTC 
GGCCCTTCTT TGCTGGGCAG GAATATAAAG CTGTATTGCA ACTATCCGAA GTCAACTAAC 
GGAATTGTGG AAGAATTCAG CAGAGATTGT TATACGTTAA TAAGATGGTC ACGGAATGAA 
GGATGCAAAA ACGCAGACGG TAGCTCAACC TTTGCACAGA TCACCCCCAC GATCGCAGGA 
AGCTTCCACT ACTACTTCGT TGAAGACGAT GAGTTTACTA AGACGAATTT CAAAAACGGA 
TCCGGCTATT TTATAGTTGA CCCTGTATTG AAATATGGTG CCAATGAGCT GTTGCCTCTG 
AACTGTATAC AGAGTCAAAC GGTATTGTCA AAACTTTTGG GGCCGTTCTC CACATGGGAA 
AACAAGATAC GGGTTGCCAA AGAATCCGGT TATAACATGG TGCATTTTAC GCCCATTCAG 
CAACTAGGAG GATCCCATTC CGCATACAGT GTCCAAGACC ACTTGAAATT AAATGTTTCT 
TTTAACGAAG AAAATGGACG AGAACCGTCA TTTGAAGATA TCGAGACGCT GACCGCAAAA 
ATGCGTGAAG ATTGGAAGAT GTTGTCCGTA TGTGACATAG TGATAAATCA CACAGCCAAT 
GAAAGTGATT GGATTAAAGA ACACCCTGAA GCAGCATACA ATTGCTTGAA CTGTCCTTAC 
ATGCGACCAG CCTATCTGTT AGATGAAGCT CTCCATCAGT TTTCCATGGA TATAAGAAAA 
GGCCTCTATG AGGACCGGGG AATCCCTCTT GACGTTTCAA CGGAAGATCA TCTAAACGCA 
ATCCGTTATC ACTTTAAACA AAACGTGTTA CAACCATTGA AAATTCATGA GCTGTTTATT 
GTAGACGTAA CCCAATACTT GGAACAGTTT TTAACACAGG CCAGAAAAAC CGCACCTCTC 
AATGACTGCG AAAGAAACCT CTTACCGGAC TTGAAAATTA TTCAAGATGT TCAGTTTAGG 
AGATTGAGCA GTTCGGTCGA CTTAAATTTG GCCCTCGCCA AGTTCAACTG CTATCGGCCA 
GATTGTTACG ATGAGGAGTC GAGAGTTAGA AGATGTTCCG AGGATTTTAA AATTCAGCTG 
GATGCCCTCA ATCAGGCTGC AATTGATGAA GTCACTGCTG ACCTTAGTGC TGCAGTTGAA 
AACGTTATTG CTGGCATTCG GTATTTCAGA GTACAGCCAG ACGGCCCTCA ATATAAGGCC 
ATTACCATTG ATCATCCATT GGTGTACAGG TACTTTACCG ATTATCATTC ACCCAAATCC 
CTGGCAGAAG CTGAAGAAAC AATGTACAGT ACAAACGGAC GGTTTCTCAT GGCCCATAAT 
GGTTGGGTGA TGAGCGCTGA GCCATTAAAG AACTTTGCCG ATGCCGGCAC CAAGATTTAC 
ATTAGAAGGG AACTTATCGC TTGGGGCGAC AGCGTTAAGC TACGCTTCGG GGACAAGCCG 
GCCGATTCTC CATTTTTGTG GAATTACATG CGGCAGTACG TAGAACAGAC GGTTAAAATA 
TTTGATGGTA TTCGGCTGGA CAACTGCCAC TCAACTTCCC TTCCCGTTGC CGAATATCTT 
TTGGACTGCG CCAGAAGAAT CCGTCCTGAT CTTTATGTGG TGGCCGAGTT GTTCACCAAC 
TCGGCCTTGA CTGACAACAT TTTCGTAAAT CGTTTGGGCA TCACGTCGCT TATTCGAGAA 
GCGATGTCCG CATGGGATGC TCATGAACAG GGACGTCTGG TATATCGATT CGGAGGTGTT 
CCAGTGGGTG CTTTTTACAA ACCTTCAAAA GTACCATTGG TCCCTATGGT GGCACATGCA 
CTTTTCCTCG ATCAAACCCA TGATAATCCC AGTCCAGTCG AAAAAAGATC TGTTTTCGAT 
TTGCTGCCGA GCGCCGGTTT AGTAAACATG GCATGTTGTG CCACCGGCTC AAATCGAGGC 
TACGACGAAC TGGTTCCCCA CCACATCCAC GTAGTGGATG AAACTAGACA ATACGCTGAG 
TGGAGTCATC ATGAAAAGCT TCCTTCCAAA ACCAGATATG TTAATAAGTG GTCAGGCATT 
ATTGCGGCCA AAAGAGCTCT GCACGAGTTG CACTTTAAGC TAGGAATGGA AGGATTCGAC 
CAGGTATTTG TGAATCAAGT AGATGGCGAT ATTGTTGAGG TGACCAGGCA CCAACCGGGC 
ACCCACACCA GTTATGTCCT AGTAGCATTC ACGGCCTTTG AACATCCCAC CGGTGACCAC 
CAGAGAGCCA TTAAACCGCT TTCTGTTGAA GGAGTTCTGG ATGAAATTGT ACTGGAAGCC 
TACTTAATTC ATGCAGATTC CACTTCCAGG TTTGGCAGAT TTGAAAAATT TGAGAAAGAC 
CCCGATTGGA TCAACGGATT AGCCGAATAT AAGCTTTCAA TTAAAGAGCA CATCCAGTTG 
CGCGAATCTG AGTTTTTCGA GGAAGCAGCT GACAGTAGTT CTAATTTCGT CCGGTTAAAC 
TATAAAAACT TCAAGCCCGG ATCAATTGCA GTGTTGAAAT TTTCTTTGCC CCAAGGAATC 
CGAGATGCGA TATCTCTGTT GAAACCCCCA CAGGCTCCAA ATCTAGAGAA AATTGTAAGT 
GAATTGACTC TAGCAGATTT GAACAGAACT CTCTTTCGAT GTGGCGAAGA AGAAAGGGAT 
GAAGGCAATG GCTTCGACAC CTACAACATA CCCAACTTTG GACCGACAAT TTATTGTGGT 
TTATTCGGCA TTACGTGGGT GCTGCGTCAA ATAGCCACAG ACAATGACCT GGGCCATCCA 
TTGTGTACCA ACTTGCGAAA TGGCAACTGG TTGATTGAAT ACGTTTTCAA ACGCCTTAAA 
GCCGATGAGG GGACCCGCGC TTTGGGGGAA TGGATTGAAG ACTACAGTAA GCCCCTCCTA 
AATATGCCCC GTTACTTAGT TCCCTGTTAT TTCGATGTCT TCATTGGGCA AATATACCCT 
CAGCTACTAA ATGCTGGCTA TCAATTGATG TCTAATTTTA TCAAGCAGGG ATCGCCTTTT 
GTTCAGTCGT TGGCTTTGGG GTCGTTGCAA TTTGGCGGTT ACCTCAGATC TGCCTGTCTG 
CCAGATATGT CGCCCAATCT GGCTCCGCCA AAACCTCCTA GCAGAAAAAA CGAAAACGGT 
GAAACAGTCC AGGCCTGCGT TACTTTATCA GCAGGATTGC CCCACTTCGC CGTGGGTTAC 
ATGCGAAATT GGGGTAGAGA CACCTTTATT GCTCTGCGCG GGCTGTTCAT CTTAACTGGC 
CGGTTTGAAG AAGCCCGACA ACATATTCTG GGTTACGGGT CGTGTTTGAG GCACGGCCTC 
ATTCCGAATT TACTGGATGC TGGTAGAAAT CCCAGGTTTA ACTGCCGAGA TGCCATTTGG 
TGGTGGCTCT ATTCGCTCAA ATCATATTGC GAAGAATCCC CTAAGGGCGT TGCTATTCTT 
GCCGATGTTG TATCACGCAT ATTTCCTTCG GATGATTCTT CTCCACAACC GCCTGGTGCT 
GTGGATCAAC CGCTATACAA TGTAATTCAG GAATCGTTAA CTGTCCATTT CCAAGGCCTA 
CAATTTCGCG AAAGGAACGC TGGACGAGAA ATTGATGAAC ACATGACCGA TGCAGGATTT 
AACAACCAAA TCGGAACTCA TCCGGAAACT GGATTCGTTT TTGGTGGGAA TGAATGGAAT 
TGCGGTACTT GGATGGACAA AATGGGTTCA TCAGACAAGG CTGGCAATCG GGGCAAACCG 
GCGTCGCCTC GTGACGGATC TGCTGTCGAA CTCGTAGGCT TATCTAAAGC CTGTATCACT 
TGGTTAATGC ACTTAAATGA TGAAGGTCAG TACCCTTATG ATGGGGTTCA GCGAACACAT 
AAGAATGGAA CGGTGACCAA ATGGACCTTC AGAGAATGGT CGGACAAAAT TCAACAAAAC 
TTCGAAAAGA AATTCTGGAT TTCAGGGAAC CCGAACCCAG AAGAATGTAG GCCTGATTTA 
GTGAATAAGA AGAACATTTA CAAAGACAGC TTCGGAGCGT CTCAGCCATG GACAGATTTT 
CAGCTTAGGT GTAATTTTCC CATTGCCATG GTCGCTGCTC CCGAGTTGTT TAATCCTCAA 
CGTGCATGGG CCGCATTAGA GCAAGTCGAA AAGTATCTCT TGGGGCCACT AGGCATGAAA 
ACGCTAGACC CCGAAGACTG GAATTACCGG GGCGACTATG ATAATTCGAT TGATTCAGAT 
GATTTACTTA CGGCCCATGG CTTCAATTAT CATCAAGGCC CTGAATGGGT TTGGCCTGTG 
GGTTTTTTCC TAAGAGCAAA GTTAATATTT GCTGCTCAGA ATGGACTGTT AGACGAAACA 
GTGACCAAAA CAAAACAAAT CTTATCAAAG CATTTTATCG AAGTGGAGAC GCCACCGTGG 
CGTGGATTAC CGGAATTAAC CAACAGCGAT GGATCCTACT GCCCGGACAG TGCGAGAACC 
CAAGCTTGGA GCATGTCCTG CATTCTCGAG GTCCTGTATG ACTTGGAGAA AATCGAATCC 
AATAGAAGTG TTGCAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019762195.1
CDS	97..5277
Translation	MIRSKMASLASWLFGRVAGAAPENQTDQNIGQATTSGKNISHASIVTNTVPVGFEASRGTSALPIKEHESASSLEASGETAKSFDDFVHVNIDEENGDSEGGLYEQTSHSDRTLNDPNRQQAAEPSFTNCSTECVDFEVENSSPKCSQIETVENLKIPDIRPGECKRELSAVENRKLNDEPEHPKYIDMASVNILILNDKANLDSELHRIQKNVKLEFRLGPSLLGRNIKLYCNYPKSTNGIVEEFSRDCYTLIRWSRNEGCKNADGSSTFAQITPTIAGSFHYYFVEDDEFTKTNFKNGSGYFIVDPVLKYGANELLPLNCIQSQTVLSKLLGPFSTWENKIRVAKESGYNMVHFTPIQQLGGSHSAYSVQDHLKLNVSFNEENGREPSFEDIETLTAKMREDWKMLSVCDIVINHTANESDWIKEHPEAAYNCLNCPYMRPAYLLDEALHQFSMDIRKGLYEDRGIPLDVSTEDHLNAIRYHFKQNVLQPLKIHELFIVDVTQYLEQFLTQARKTAPLNDCERNLLPDLKIIQDVQFRRLSSSVDLNLALAKFNCYRPDCYDEESRVRRCSEDFKIQLDALNQAAIDEVTADLSAAVENVIAGIRYFRVQPDGPQYKAITIDHPLVYRYFTDYHSPKSLAEAEETMYSTNGRFLMAHNGWVMSAEPLKNFADAGTKIYIRRELIAWGDSVKLRFGDKPADSPFLWNYMRQYVEQTVKIFDGIRLDNCHSTSLPVAEYLLDCARRIRPDLYVVAELFTNSALTDNIFVNRLGITSLIREAMSAWDAHEQGRLVYRFGGVPVGAFYKPSKVPLVPMVAHALFLDQTHDNPSPVEKRSVFDLLPSAGLVNMACCATGSNRGYDELVPHHIHVVDETRQYAEWSHHEKLPSKTRYVNKWSGIIAAKRALHELHFKLGMEGFDQVFVNQVDGDIVEVTRHQPGTHTSYVLVAFTAFEHPTGDHQRAIKPLSVEGVLDEIVLEAYLIHADSTSRFGRFEKFEKDPDWINGLAEYKLSIKEHIQLRESEFFEEAADSSSNFVRLNYKNFKPGSIAVLKFSLPQGIRDAISLLKPPQAPNLEKIVSELTLADLNRTLFRCGEEERDEGNGFDTYNIPNFGPTIYCGLFGITWVLRQIATDNDLGHPLCTNLRNGNWLIEYVFKRLKADEGTRALGEWIEDYSKPLLNMPRYLVPCYFDVFIGQIYPQLLNAGYQLMSNFIKQGSPFVQSLALGSLQFGGYLRSACLPDMSPNLAPPKPPSRKNENGETVQACVTLSAGLPHFAVGYMRNWGRDTFIALRGLFILTGRFEEARQHILGYGSCLRHGLIPNLLDAGRNPRFNCRDAIWWWLYSLKSYCEESPKGVAILADVVSRIFPSDDSSPQPPGAVDQPLYNVIQESLTVHFQGLQFRERNAGREIDEHMTDAGFNNQIGTHPETGFVFGGNEWNCGTWMDKMGSSDKAGNRGKPASPRDGSAVELVGLSKACITWLMHLNDEGQYPYDGVQRTHKNGTVTKWTFREWSDKIQQNFEKKFWISGNPNPEECRPDLVNKKNIYKDSFGASQPWTDFQLRCNFPIAMVAAPELFNPQRAWAALEQVEKYLLGPLGMKTLDPEDWNYRGDYDNSIDSDDLLTAHGFNYHQGPEWVWPVGFFLRAKLIFAAQNGLLDETVTKTKQILSKHFIEVETPPWRGLPELTNSDGSYCPDSARTQAWSMSCILEVLYDLEKIESNRSVAN

Target ORF information:

RefSeq Version	XM_019906636.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019906636.1

ORF Insert Sequence:

ATGATCCGAT CTAAAATGGC ATCATTGGCA TCATGGCTCT TCGGCAGAGT TGCAGGTGCC 
GCACCAGAAA ACCAAACTGA CCAGAACATC GGTCAAGCTA CAACTTCTGG TAAAAACATC 
TCGCATGCCA GCATAGTCAC CAATACTGTT CCCGTCGGTT TTGAGGCTTC TCGTGGTACC 
TCGGCCCTAC CCATCAAAGA ACACGAAAGT GCGAGCTCTC TTGAAGCATC GGGTGAAACT 
GCAAAAAGTT TTGACGATTT CGTGCACGTA AACATTGATG AAGAAAACGG TGATTCAGAA 
GGCGGCTTGT ATGAACAGAC TTCACATAGC GATCGAACGC TGAATGATCC CAATCGGCAG 
CAAGCAGCCG AACCAAGTTT TACAAACTGC AGTACCGAAT GCGTAGATTT CGAAGTTGAA 
AACTCTAGTC CTAAATGTTC ACAAATAGAA ACTGTTGAAA ATTTGAAAAT TCCAGACATT 
CGACCAGGTG AATGTAAGAG AGAACTAAGC GCCGTTGAAA ACCGAAAGCT CAACGACGAG 
CCGGAACATC CAAAATATAT CGACATGGCA TCTGTAAATA TATTGATACT GAACGATAAA 
GCAAACTTGG ATTCCGAGCT TCACAGAATT CAAAAAAATG TTAAATTGGA GTTCAGACTC 
GGCCCTTCTT TGCTGGGCAG GAATATAAAG CTGTATTGCA ACTATCCGAA GTCAACTAAC 
GGAATTGTGG AAGAATTCAG CAGAGATTGT TATACGTTAA TAAGATGGTC ACGGAATGAA 
GGATGCAAAA ACGCAGACGG TAGCTCAACC TTTGCACAGA TCACCCCCAC GATCGCAGGA 
AGCTTCCACT ACTACTTCGT TGAAGACGAT GAGTTTACTA AGACGAATTT CAAAAACGGA 
TCCGGCTATT TTATAGTTGA CCCTGTATTG AAATATGGTG CCAATGAGCT GTTGCCTCTG 
AACTGTATAC AGAGTCAAAC GGTATTGTCA AAACTTTTGG GGCCGTTCTC CACATGGGAA 
AACAAGATAC GGGTTGCCAA AGAATCCGGT TATAACATGG TGCATTTTAC GCCCATTCAG 
CAACTAGGAG GATCCCATTC CGCATACAGT GTCCAAGACC ACTTGAAATT AAATGTTTCT 
TTTAACGAAG AAAATGGACG AGAACCGTCA TTTGAAGATA TCGAGACGCT GACCGCAAAA 
ATGCGTGAAG ATTGGAAGAT GTTGTCCGTA TGTGACATAG TGATAAATCA CACAGCCAAT 
GAAAGTGATT GGATTAAAGA ACACCCTGAA GCAGCATACA ATTGCTTGAA CTGTCCTTAC 
ATGCGACCAG CCTATCTGTT AGATGAAGCT CTCCATCAGT TTTCCATGGA TATAAGAAAA 
GGCCTCTATG AGGACCGGGG AATCCCTCTT GACGTTTCAA CGGAAGATCA TCTAAACGCA 
ATCCGTTATC ACTTTAAACA AAACGTGTTA CAACCATTGA AAATTCATGA GCTGTTTATT 
GTAGACGTAA CCCAATACTT GGAACAGTTT TTAACACAGG CCAGAAAAAC CGCACCTCTC 
AATGACTGCG AAAGAAACCT CTTACCGGAC TTGAAAATTA TTCAAGATGT TCAGTTTAGG 
AGATTGAGCA GTTCGGTCGA CTTAAATTTG GCCCTCGCCA AGTTCAACTG CTATCGGCCA 
GATTGTTACG ATGAGGAGTC GAGAGTTAGA AGATGTTCCG AGGATTTTAA AATTCAGCTG 
GATGCCCTCA ATCAGGCTGC AATTGATGAA GTCACTGCTG ACCTTAGTGC TGCAGTTGAA 
AACGTTATTG CTGGCATTCG GTATTTCAGA GTACAGCCAG ACGGCCCTCA ATATAAGGCC 
ATTACCATTG ATCATCCATT GGTGTACAGG TACTTTACCG ATTATCATTC ACCCAAATCC 
CTGGCAGAAG CTGAAGAAAC AATGTACAGT ACAAACGGAC GGTTTCTCAT GGCCCATAAT 
GGTTGGGTGA TGAGCGCTGA GCCATTAAAG AACTTTGCCG ATGCCGGCAC CAAGATTTAC 
ATTAGAAGGG AACTTATCGC TTGGGGCGAC AGCGTTAAGC TACGCTTCGG GGACAAGCCG 
GCCGATTCTC CATTTTTGTG GAATTACATG CGGCAGTACG TAGAACAGAC GGTTAAAATA 
TTTGATGGTA TTCGGCTGGA CAACTGCCAC TCAACTTCCC TTCCCGTTGC CGAATATCTT 
TTGGACTGCG CCAGAAGAAT CCGTCCTGAT CTTTATGTGG TGGCCGAGTT GTTCACCAAC 
TCGGCCTTGA CTGACAACAT TTTCGTAAAT CGTTTGGGCA TCACGTCGCT TATTCGAGAA 
GCGATGTCCG CATGGGATGC TCATGAACAG GGACGTCTGG TATATCGATT CGGAGGTGTT 
CCAGTGGGTG CTTTTTACAA ACCTTCAAAA GTACCATTGG TCCCTATGGT GGCACATGCA 
CTTTTCCTCG ATCAAACCCA TGATAATCCC AGTCCAGTCG AAAAAAGATC TGTTTTCGAT 
TTGCTGCCGA GCGCCGGTTT AGTAAACATG GCATGTTGTG CCACCGGCTC AAATCGAGGC 
TACGACGAAC TGGTTCCCCA CCACATCCAC GTAGTGGATG AAACTAGACA ATACGCTGAG 
TGGAGTCATC ATGAAAAGCT TCCTTCCAAA ACCAGATATG TTAATAAGTG GTCAGGCATT 
ATTGCGGCCA AAAGAGCTCT GCACGAGTTG CACTTTAAGC TAGGAATGGA AGGATTCGAC 
CAGGTATTTG TGAATCAAGT AGATGGCGAT ATTGTTGAGG TGACCAGGCA CCAACCGGGC 
ACCCACACCA GTTATGTCCT AGTAGCATTC ACGGCCTTTG AACATCCCAC CGGTGACCAC 
CAGAGAGCCA TTAAACCGCT TTCTGTTGAA GGAGTTCTGG ATGAAATTGT ACTGGAAGCC 
TACTTAATTC ATGCAGATTC CACTTCCAGG TTTGGCAGAT TTGAAAAATT TGAGAAAGAC 
CCCGATTGGA TCAACGGATT AGCCGAATAT AAGCTTTCAA TTAAAGAGCA CATCCAGTTG 
CGCGAATCTG AGTTTTTCGA GGAAGCAGCT GACAGTAGTT CTAATTTCGT CCGGTTAAAC 
TATAAAAACT TCAAGCCCGG ATCAATTGCA GTGTTGAAAT TTTCTTTGCC CCAAGGAATC 
CGAGATGCGA TATCTCTGTT GAAACCCCCA CAGGCTCCAA ATCTAGAGAA AATTGTAAGT 
GAATTGACTC TAGCAGATTT GAACAGAACT CTCTTTCGAT GTGGCGAAGA AGAAAGGGAT 
GAAGGCAATG GCTTCGACAC CTACAACATA CCCAACTTTG GACCGACAAT TTATTGTGGT 
TTATTCGGCA TTACGTGGGT GCTGCGTCAA ATAGCCACAG ACAATGACCT GGGCCATCCA 
TTGTGTACCA ACTTGCGAAA TGGCAACTGG TTGATTGAAT ACGTTTTCAA ACGCCTTAAA 
GCCGATGAGG GGACCCGCGC TTTGGGGGAA TGGATTGAAG ACTACAGTAA GCCCCTCCTA 
AATATGCCCC GTTACTTAGT TCCCTGTTAT TTCGATGTCT TCATTGGGCA AATATACCCT 
CAGCTACTAA ATGCTGGCTA TCAATTGATG TCTAATTTTA TCAAGCAGGG ATCGCCTTTT 
GTTCAGTCGT TGGCTTTGGG GTCGTTGCAA TTTGGCGGTT ACCTCAGATC TGCCTGTCTG 
CCAGATATGT CGCCCAATCT GGCTCCGCCA AAACCTCCTA GCAGAAAAAA CGAAAACGGT 
GAAACAGTCC AGGCCTGCGT TACTTTATCA GCAGGATTGC CCCACTTCGC CGTGGGTTAC 
ATGCGAAATT GGGGTAGAGA CACCTTTATT GCTCTGCGCG GGCTGTTCAT CTTAACTGGC 
CGGTTTGAAG AAGCCCGACA ACATATTCTG GGTTACGGGT CGTGTTTGAG GCACGGCCTC 
ATTCCGAATT TACTGGATGC TGGTAGAAAT CCCAGGTTTA ACTGCCGAGA TGCCATTTGG 
TGGTGGCTCT ATTCGCTCAA ATCATATTGC GAAGAATCCC CTAAGGGCGT TGCTATTCTT 
GCCGATGTTG TATCACGCAT ATTTCCTTCG GATGATTCTT CTCCACAACC GCCTGGTGCT 
GTGGATCAAC CGCTATACAA TGTAATTCAG GAATCGTTAA CTGTCCATTT CCAAGGCCTA 
CAATTTCGCG AAAGGAACGC TGGACGAGAA ATTGATGAAC ACATGACCGA TGCAGGATTT 
AACAACCAAA TCGGAACTCA TCCGGAAACT GGATTCGTTT TTGGTGGGAA TGAATGGAAT 
TGCGGTACTT GGATGGACAA AATGGGTTCA TCAGACAAGG CTGGCAATCG GGGCAAACCG 
GCGTCGCCTC GTGACGGATC TGCTGTCGAA CTCGTAGGCT TATCTAAAGC CTGTATCACT 
TGGTTAATGC ACTTAAATGA TGAAGGTCAG TACCCTTATG ATGGGGTTCA GCGAACACAT 
AAGAATGGAA CGGTGACCAA ATGGACCTTC AGAGAATGGT CGGACAAAAT TCAACAAAAC 
TTCGAAAAGA AATTCTGGAT TTCAGGGAAC CCGAACCCAG AAGAATGTAG GCCTGATTTA 
GTGAATAAGA AGAACATTTA CAAAGACAGC TTCGGAGCGT CTCAGCCATG GACAGATTTT 
CAGCTTAGGT GTAATTTTCC CATTGCCATG GTCGCTGCTC CCGAGTTGTT TAATCCTCAA 
CGTGCATGGG CCGCATTAGA GCAAGTCGAA AAGTATCTCT TGGGGCCACT AGGCATGAAA 
ACGCTAGACC CCGAAGACTG GAATTACCGG GGCGACTATG ATAATTCGAT TGATTCAGAT 
GATTTACTTA CGGCCCATGG CTTCAATTAT CATCAAGGCC CTGAATGGGT TTGGCCTGTG 
GGTTTTTTCC TAAGAGCAAA GTTAATATTT GCTGCTCAGA ATGGACTGTT AGACGAAACA 
GTGACCAAAA CAAAACAAAT CTTATCAAAG CATTTTATCG AAGTGGAGAC GCCACCGTGG 
CGTGGATTAC CGGAATTAAC CAACAGCGAT GGATCCTACT GCCCGGACAG TGCGAGAACC 
CAAGCTTGGA GCATGTCCTG CATTCTCGAG GTCCTGTATG ACTTGGAGAA AATCGAATCC 
AATAGAAGTG TTGCAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109539064 ( XM_019906635.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019762194.1 Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X4, mRNA.

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CloneID	ODe42980
Clone ID Related Accession (Same CDS sequence)	XM_019906636.1 , XM_019906635.1
Accession Version	XM_019906635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	glycogen debranching enzyme isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCGAT CTAAAATGGC ATCATTGGCA TCATGGCTCT TCGGCAGAGT TGCAGGTGCC 
GCACCAGAAA ACCAAACTGA CCAGAACATC GGTCAAGCTA CAACTTCTGG TAAAAACATC 
TCGCATGCCA GCATAGTCAC CAATACTGTT CCCGTCGGTT TTGAGGCTTC TCGTGGTACC 
TCGGCCCTAC CCATCAAAGA ACACGAAAGT GCGAGCTCTC TTGAAGCATC GGGTGAAACT 
GCAAAAAGTT TTGACGATTT CGTGCACGTA AACATTGATG AAGAAAACGG TGATTCAGAA 
GGCGGCTTGT ATGAACAGAC TTCACATAGC GATCGAACGC TGAATGATCC CAATCGGCAG 
CAAGCAGCCG AACCAAGTTT TACAAACTGC AGTACCGAAT GCGTAGATTT CGAAGTTGAA 
AACTCTAGTC CTAAATGTTC ACAAATAGAA ACTGTTGAAA ATTTGAAAAT TCCAGACATT 
CGACCAGGTG AATGTAAGAG AGAACTAAGC GCCGTTGAAA ACCGAAAGCT CAACGACGAG 
CCGGAACATC CAAAATATAT CGACATGGCA TCTGTAAATA TATTGATACT GAACGATAAA 
GCAAACTTGG ATTCCGAGCT TCACAGAATT CAAAAAAATG TTAAATTGGA GTTCAGACTC 
GGCCCTTCTT TGCTGGGCAG GAATATAAAG CTGTATTGCA ACTATCCGAA GTCAACTAAC 
GGAATTGTGG AAGAATTCAG CAGAGATTGT TATACGTTAA TAAGATGGTC ACGGAATGAA 
GGATGCAAAA ACGCAGACGG TAGCTCAACC TTTGCACAGA TCACCCCCAC GATCGCAGGA 
AGCTTCCACT ACTACTTCGT TGAAGACGAT GAGTTTACTA AGACGAATTT CAAAAACGGA 
TCCGGCTATT TTATAGTTGA CCCTGTATTG AAATATGGTG CCAATGAGCT GTTGCCTCTG 
AACTGTATAC AGAGTCAAAC GGTATTGTCA AAACTTTTGG GGCCGTTCTC CACATGGGAA 
AACAAGATAC GGGTTGCCAA AGAATCCGGT TATAACATGG TGCATTTTAC GCCCATTCAG 
CAACTAGGAG GATCCCATTC CGCATACAGT GTCCAAGACC ACTTGAAATT AAATGTTTCT 
TTTAACGAAG AAAATGGACG AGAACCGTCA TTTGAAGATA TCGAGACGCT GACCGCAAAA 
ATGCGTGAAG ATTGGAAGAT GTTGTCCGTA TGTGACATAG TGATAAATCA CACAGCCAAT 
GAAAGTGATT GGATTAAAGA ACACCCTGAA GCAGCATACA ATTGCTTGAA CTGTCCTTAC 
ATGCGACCAG CCTATCTGTT AGATGAAGCT CTCCATCAGT TTTCCATGGA TATAAGAAAA 
GGCCTCTATG AGGACCGGGG AATCCCTCTT GACGTTTCAA CGGAAGATCA TCTAAACGCA 
ATCCGTTATC ACTTTAAACA AAACGTGTTA CAACCATTGA AAATTCATGA GCTGTTTATT 
GTAGACGTAA CCCAATACTT GGAACAGTTT TTAACACAGG CCAGAAAAAC CGCACCTCTC 
AATGACTGCG AAAGAAACCT CTTACCGGAC TTGAAAATTA TTCAAGATGT TCAGTTTAGG 
AGATTGAGCA GTTCGGTCGA CTTAAATTTG GCCCTCGCCA AGTTCAACTG CTATCGGCCA 
GATTGTTACG ATGAGGAGTC GAGAGTTAGA AGATGTTCCG AGGATTTTAA AATTCAGCTG 
GATGCCCTCA ATCAGGCTGC AATTGATGAA GTCACTGCTG ACCTTAGTGC TGCAGTTGAA 
AACGTTATTG CTGGCATTCG GTATTTCAGA GTACAGCCAG ACGGCCCTCA ATATAAGGCC 
ATTACCATTG ATCATCCATT GGTGTACAGG TACTTTACCG ATTATCATTC ACCCAAATCC 
CTGGCAGAAG CTGAAGAAAC AATGTACAGT ACAAACGGAC GGTTTCTCAT GGCCCATAAT 
GGTTGGGTGA TGAGCGCTGA GCCATTAAAG AACTTTGCCG ATGCCGGCAC CAAGATTTAC 
ATTAGAAGGG AACTTATCGC TTGGGGCGAC AGCGTTAAGC TACGCTTCGG GGACAAGCCG 
GCCGATTCTC CATTTTTGTG GAATTACATG CGGCAGTACG TAGAACAGAC GGTTAAAATA 
TTTGATGGTA TTCGGCTGGA CAACTGCCAC TCAACTTCCC TTCCCGTTGC CGAATATCTT 
TTGGACTGCG CCAGAAGAAT CCGTCCTGAT CTTTATGTGG TGGCCGAGTT GTTCACCAAC 
TCGGCCTTGA CTGACAACAT TTTCGTAAAT CGTTTGGGCA TCACGTCGCT TATTCGAGAA 
GCGATGTCCG CATGGGATGC TCATGAACAG GGACGTCTGG TATATCGATT CGGAGGTGTT 
CCAGTGGGTG CTTTTTACAA ACCTTCAAAA GTACCATTGG TCCCTATGGT GGCACATGCA 
CTTTTCCTCG ATCAAACCCA TGATAATCCC AGTCCAGTCG AAAAAAGATC TGTTTTCGAT 
TTGCTGCCGA GCGCCGGTTT AGTAAACATG GCATGTTGTG CCACCGGCTC AAATCGAGGC 
TACGACGAAC TGGTTCCCCA CCACATCCAC GTAGTGGATG AAACTAGACA ATACGCTGAG 
TGGAGTCATC ATGAAAAGCT TCCTTCCAAA ACCAGATATG TTAATAAGTG GTCAGGCATT 
ATTGCGGCCA AAAGAGCTCT GCACGAGTTG CACTTTAAGC TAGGAATGGA AGGATTCGAC 
CAGGTATTTG TGAATCAAGT AGATGGCGAT ATTGTTGAGG TGACCAGGCA CCAACCGGGC 
ACCCACACCA GTTATGTCCT AGTAGCATTC ACGGCCTTTG AACATCCCAC CGGTGACCAC 
CAGAGAGCCA TTAAACCGCT TTCTGTTGAA GGAGTTCTGG ATGAAATTGT ACTGGAAGCC 
TACTTAATTC ATGCAGATTC CACTTCCAGG TTTGGCAGAT TTGAAAAATT TGAGAAAGAC 
CCCGATTGGA TCAACGGATT AGCCGAATAT AAGCTTTCAA TTAAAGAGCA CATCCAGTTG 
CGCGAATCTG AGTTTTTCGA GGAAGCAGCT GACAGTAGTT CTAATTTCGT CCGGTTAAAC 
TATAAAAACT TCAAGCCCGG ATCAATTGCA GTGTTGAAAT TTTCTTTGCC CCAAGGAATC 
CGAGATGCGA TATCTCTGTT GAAACCCCCA CAGGCTCCAA ATCTAGAGAA AATTGTAAGT 
GAATTGACTC TAGCAGATTT GAACAGAACT CTCTTTCGAT GTGGCGAAGA AGAAAGGGAT 
GAAGGCAATG GCTTCGACAC CTACAACATA CCCAACTTTG GACCGACAAT TTATTGTGGT 
TTATTCGGCA TTACGTGGGT GCTGCGTCAA ATAGCCACAG ACAATGACCT GGGCCATCCA 
TTGTGTACCA ACTTGCGAAA TGGCAACTGG TTGATTGAAT ACGTTTTCAA ACGCCTTAAA 
GCCGATGAGG GGACCCGCGC TTTGGGGGAA TGGATTGAAG ACTACAGTAA GCCCCTCCTA 
AATATGCCCC GTTACTTAGT TCCCTGTTAT TTCGATGTCT TCATTGGGCA AATATACCCT 
CAGCTACTAA ATGCTGGCTA TCAATTGATG TCTAATTTTA TCAAGCAGGG ATCGCCTTTT 
GTTCAGTCGT TGGCTTTGGG GTCGTTGCAA TTTGGCGGTT ACCTCAGATC TGCCTGTCTG 
CCAGATATGT CGCCCAATCT GGCTCCGCCA AAACCTCCTA GCAGAAAAAA CGAAAACGGT 
GAAACAGTCC AGGCCTGCGT TACTTTATCA GCAGGATTGC CCCACTTCGC CGTGGGTTAC 
ATGCGAAATT GGGGTAGAGA CACCTTTATT GCTCTGCGCG GGCTGTTCAT CTTAACTGGC 
CGGTTTGAAG AAGCCCGACA ACATATTCTG GGTTACGGGT CGTGTTTGAG GCACGGCCTC 
ATTCCGAATT TACTGGATGC TGGTAGAAAT CCCAGGTTTA ACTGCCGAGA TGCCATTTGG 
TGGTGGCTCT ATTCGCTCAA ATCATATTGC GAAGAATCCC CTAAGGGCGT TGCTATTCTT 
GCCGATGTTG TATCACGCAT ATTTCCTTCG GATGATTCTT CTCCACAACC GCCTGGTGCT 
GTGGATCAAC CGCTATACAA TGTAATTCAG GAATCGTTAA CTGTCCATTT CCAAGGCCTA 
CAATTTCGCG AAAGGAACGC TGGACGAGAA ATTGATGAAC ACATGACCGA TGCAGGATTT 
AACAACCAAA TCGGAACTCA TCCGGAAACT GGATTCGTTT TTGGTGGGAA TGAATGGAAT 
TGCGGTACTT GGATGGACAA AATGGGTTCA TCAGACAAGG CTGGCAATCG GGGCAAACCG 
GCGTCGCCTC GTGACGGATC TGCTGTCGAA CTCGTAGGCT TATCTAAAGC CTGTATCACT 
TGGTTAATGC ACTTAAATGA TGAAGGTCAG TACCCTTATG ATGGGGTTCA GCGAACACAT 
AAGAATGGAA CGGTGACCAA ATGGACCTTC AGAGAATGGT CGGACAAAAT TCAACAAAAC 
TTCGAAAAGA AATTCTGGAT TTCAGGGAAC CCGAACCCAG AAGAATGTAG GCCTGATTTA 
GTGAATAAGA AGAACATTTA CAAAGACAGC TTCGGAGCGT CTCAGCCATG GACAGATTTT 
CAGCTTAGGT GTAATTTTCC CATTGCCATG GTCGCTGCTC CCGAGTTGTT TAATCCTCAA 
CGTGCATGGG CCGCATTAGA GCAAGTCGAA AAGTATCTCT TGGGGCCACT AGGCATGAAA 
ACGCTAGACC CCGAAGACTG GAATTACCGG GGCGACTATG ATAATTCGAT TGATTCAGAT 
GATTTACTTA CGGCCCATGG CTTCAATTAT CATCAAGGCC CTGAATGGGT TTGGCCTGTG 
GGTTTTTTCC TAAGAGCAAA GTTAATATTT GCTGCTCAGA ATGGACTGTT AGACGAAACA 
GTGACCAAAA CAAAACAAAT CTTATCAAAG CATTTTATCG AAGTGGAGAC GCCACCGTGG 
CGTGGATTAC CGGAATTAAC CAACAGCGAT GGATCCTACT GCCCGGACAG TGCGAGAACC 
CAAGCTTGGA GCATGTCCTG CATTCTCGAG GTCCTGTATG ACTTGGAGAA AATCGAATCC 
AATAGAAGTG TTGCAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019762194.1
CDS	17..5197
Translation	MIRSKMASLASWLFGRVAGAAPENQTDQNIGQATTSGKNISHASIVTNTVPVGFEASRGTSALPIKEHESASSLEASGETAKSFDDFVHVNIDEENGDSEGGLYEQTSHSDRTLNDPNRQQAAEPSFTNCSTECVDFEVENSSPKCSQIETVENLKIPDIRPGECKRELSAVENRKLNDEPEHPKYIDMASVNILILNDKANLDSELHRIQKNVKLEFRLGPSLLGRNIKLYCNYPKSTNGIVEEFSRDCYTLIRWSRNEGCKNADGSSTFAQITPTIAGSFHYYFVEDDEFTKTNFKNGSGYFIVDPVLKYGANELLPLNCIQSQTVLSKLLGPFSTWENKIRVAKESGYNMVHFTPIQQLGGSHSAYSVQDHLKLNVSFNEENGREPSFEDIETLTAKMREDWKMLSVCDIVINHTANESDWIKEHPEAAYNCLNCPYMRPAYLLDEALHQFSMDIRKGLYEDRGIPLDVSTEDHLNAIRYHFKQNVLQPLKIHELFIVDVTQYLEQFLTQARKTAPLNDCERNLLPDLKIIQDVQFRRLSSSVDLNLALAKFNCYRPDCYDEESRVRRCSEDFKIQLDALNQAAIDEVTADLSAAVENVIAGIRYFRVQPDGPQYKAITIDHPLVYRYFTDYHSPKSLAEAEETMYSTNGRFLMAHNGWVMSAEPLKNFADAGTKIYIRRELIAWGDSVKLRFGDKPADSPFLWNYMRQYVEQTVKIFDGIRLDNCHSTSLPVAEYLLDCARRIRPDLYVVAELFTNSALTDNIFVNRLGITSLIREAMSAWDAHEQGRLVYRFGGVPVGAFYKPSKVPLVPMVAHALFLDQTHDNPSPVEKRSVFDLLPSAGLVNMACCATGSNRGYDELVPHHIHVVDETRQYAEWSHHEKLPSKTRYVNKWSGIIAAKRALHELHFKLGMEGFDQVFVNQVDGDIVEVTRHQPGTHTSYVLVAFTAFEHPTGDHQRAIKPLSVEGVLDEIVLEAYLIHADSTSRFGRFEKFEKDPDWINGLAEYKLSIKEHIQLRESEFFEEAADSSSNFVRLNYKNFKPGSIAVLKFSLPQGIRDAISLLKPPQAPNLEKIVSELTLADLNRTLFRCGEEERDEGNGFDTYNIPNFGPTIYCGLFGITWVLRQIATDNDLGHPLCTNLRNGNWLIEYVFKRLKADEGTRALGEWIEDYSKPLLNMPRYLVPCYFDVFIGQIYPQLLNAGYQLMSNFIKQGSPFVQSLALGSLQFGGYLRSACLPDMSPNLAPPKPPSRKNENGETVQACVTLSAGLPHFAVGYMRNWGRDTFIALRGLFILTGRFEEARQHILGYGSCLRHGLIPNLLDAGRNPRFNCRDAIWWWLYSLKSYCEESPKGVAILADVVSRIFPSDDSSPQPPGAVDQPLYNVIQESLTVHFQGLQFRERNAGREIDEHMTDAGFNNQIGTHPETGFVFGGNEWNCGTWMDKMGSSDKAGNRGKPASPRDGSAVELVGLSKACITWLMHLNDEGQYPYDGVQRTHKNGTVTKWTFREWSDKIQQNFEKKFWISGNPNPEECRPDLVNKKNIYKDSFGASQPWTDFQLRCNFPIAMVAAPELFNPQRAWAALEQVEKYLLGPLGMKTLDPEDWNYRGDYDNSIDSDDLLTAHGFNYHQGPEWVWPVGFFLRAKLIFAAQNGLLDETVTKTKQILSKHFIEVETPPWRGLPELTNSDGSYCPDSARTQAWSMSCILEVLYDLEKIESNRSVAN

Target ORF information:

RefSeq Version	XM_019906635.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019906635.1

ORF Insert Sequence:

ATGATCCGAT CTAAAATGGC ATCATTGGCA TCATGGCTCT TCGGCAGAGT TGCAGGTGCC 
GCACCAGAAA ACCAAACTGA CCAGAACATC GGTCAAGCTA CAACTTCTGG TAAAAACATC 
TCGCATGCCA GCATAGTCAC CAATACTGTT CCCGTCGGTT TTGAGGCTTC TCGTGGTACC 
TCGGCCCTAC CCATCAAAGA ACACGAAAGT GCGAGCTCTC TTGAAGCATC GGGTGAAACT 
GCAAAAAGTT TTGACGATTT CGTGCACGTA AACATTGATG AAGAAAACGG TGATTCAGAA 
GGCGGCTTGT ATGAACAGAC TTCACATAGC GATCGAACGC TGAATGATCC CAATCGGCAG 
CAAGCAGCCG AACCAAGTTT TACAAACTGC AGTACCGAAT GCGTAGATTT CGAAGTTGAA 
AACTCTAGTC CTAAATGTTC ACAAATAGAA ACTGTTGAAA ATTTGAAAAT TCCAGACATT 
CGACCAGGTG AATGTAAGAG AGAACTAAGC GCCGTTGAAA ACCGAAAGCT CAACGACGAG 
CCGGAACATC CAAAATATAT CGACATGGCA TCTGTAAATA TATTGATACT GAACGATAAA 
GCAAACTTGG ATTCCGAGCT TCACAGAATT CAAAAAAATG TTAAATTGGA GTTCAGACTC 
GGCCCTTCTT TGCTGGGCAG GAATATAAAG CTGTATTGCA ACTATCCGAA GTCAACTAAC 
GGAATTGTGG AAGAATTCAG CAGAGATTGT TATACGTTAA TAAGATGGTC ACGGAATGAA 
GGATGCAAAA ACGCAGACGG TAGCTCAACC TTTGCACAGA TCACCCCCAC GATCGCAGGA 
AGCTTCCACT ACTACTTCGT TGAAGACGAT GAGTTTACTA AGACGAATTT CAAAAACGGA 
TCCGGCTATT TTATAGTTGA CCCTGTATTG AAATATGGTG CCAATGAGCT GTTGCCTCTG 
AACTGTATAC AGAGTCAAAC GGTATTGTCA AAACTTTTGG GGCCGTTCTC CACATGGGAA 
AACAAGATAC GGGTTGCCAA AGAATCCGGT TATAACATGG TGCATTTTAC GCCCATTCAG 
CAACTAGGAG GATCCCATTC CGCATACAGT GTCCAAGACC ACTTGAAATT AAATGTTTCT 
TTTAACGAAG AAAATGGACG AGAACCGTCA TTTGAAGATA TCGAGACGCT GACCGCAAAA 
ATGCGTGAAG ATTGGAAGAT GTTGTCCGTA TGTGACATAG TGATAAATCA CACAGCCAAT 
GAAAGTGATT GGATTAAAGA ACACCCTGAA GCAGCATACA ATTGCTTGAA CTGTCCTTAC 
ATGCGACCAG CCTATCTGTT AGATGAAGCT CTCCATCAGT TTTCCATGGA TATAAGAAAA 
GGCCTCTATG AGGACCGGGG AATCCCTCTT GACGTTTCAA CGGAAGATCA TCTAAACGCA 
ATCCGTTATC ACTTTAAACA AAACGTGTTA CAACCATTGA AAATTCATGA GCTGTTTATT 
GTAGACGTAA CCCAATACTT GGAACAGTTT TTAACACAGG CCAGAAAAAC CGCACCTCTC 
AATGACTGCG AAAGAAACCT CTTACCGGAC TTGAAAATTA TTCAAGATGT TCAGTTTAGG 
AGATTGAGCA GTTCGGTCGA CTTAAATTTG GCCCTCGCCA AGTTCAACTG CTATCGGCCA 
GATTGTTACG ATGAGGAGTC GAGAGTTAGA AGATGTTCCG AGGATTTTAA AATTCAGCTG 
GATGCCCTCA ATCAGGCTGC AATTGATGAA GTCACTGCTG ACCTTAGTGC TGCAGTTGAA 
AACGTTATTG CTGGCATTCG GTATTTCAGA GTACAGCCAG ACGGCCCTCA ATATAAGGCC 
ATTACCATTG ATCATCCATT GGTGTACAGG TACTTTACCG ATTATCATTC ACCCAAATCC 
CTGGCAGAAG CTGAAGAAAC AATGTACAGT ACAAACGGAC GGTTTCTCAT GGCCCATAAT 
GGTTGGGTGA TGAGCGCTGA GCCATTAAAG AACTTTGCCG ATGCCGGCAC CAAGATTTAC 
ATTAGAAGGG AACTTATCGC TTGGGGCGAC AGCGTTAAGC TACGCTTCGG GGACAAGCCG 
GCCGATTCTC CATTTTTGTG GAATTACATG CGGCAGTACG TAGAACAGAC GGTTAAAATA 
TTTGATGGTA TTCGGCTGGA CAACTGCCAC TCAACTTCCC TTCCCGTTGC CGAATATCTT 
TTGGACTGCG CCAGAAGAAT CCGTCCTGAT CTTTATGTGG TGGCCGAGTT GTTCACCAAC 
TCGGCCTTGA CTGACAACAT TTTCGTAAAT CGTTTGGGCA TCACGTCGCT TATTCGAGAA 
GCGATGTCCG CATGGGATGC TCATGAACAG GGACGTCTGG TATATCGATT CGGAGGTGTT 
CCAGTGGGTG CTTTTTACAA ACCTTCAAAA GTACCATTGG TCCCTATGGT GGCACATGCA 
CTTTTCCTCG ATCAAACCCA TGATAATCCC AGTCCAGTCG AAAAAAGATC TGTTTTCGAT 
TTGCTGCCGA GCGCCGGTTT AGTAAACATG GCATGTTGTG CCACCGGCTC AAATCGAGGC 
TACGACGAAC TGGTTCCCCA CCACATCCAC GTAGTGGATG AAACTAGACA ATACGCTGAG 
TGGAGTCATC ATGAAAAGCT TCCTTCCAAA ACCAGATATG TTAATAAGTG GTCAGGCATT 
ATTGCGGCCA AAAGAGCTCT GCACGAGTTG CACTTTAAGC TAGGAATGGA AGGATTCGAC 
CAGGTATTTG TGAATCAAGT AGATGGCGAT ATTGTTGAGG TGACCAGGCA CCAACCGGGC 
ACCCACACCA GTTATGTCCT AGTAGCATTC ACGGCCTTTG AACATCCCAC CGGTGACCAC 
CAGAGAGCCA TTAAACCGCT TTCTGTTGAA GGAGTTCTGG ATGAAATTGT ACTGGAAGCC 
TACTTAATTC ATGCAGATTC CACTTCCAGG TTTGGCAGAT TTGAAAAATT TGAGAAAGAC 
CCCGATTGGA TCAACGGATT AGCCGAATAT AAGCTTTCAA TTAAAGAGCA CATCCAGTTG 
CGCGAATCTG AGTTTTTCGA GGAAGCAGCT GACAGTAGTT CTAATTTCGT CCGGTTAAAC 
TATAAAAACT TCAAGCCCGG ATCAATTGCA GTGTTGAAAT TTTCTTTGCC CCAAGGAATC 
CGAGATGCGA TATCTCTGTT GAAACCCCCA CAGGCTCCAA ATCTAGAGAA AATTGTAAGT 
GAATTGACTC TAGCAGATTT GAACAGAACT CTCTTTCGAT GTGGCGAAGA AGAAAGGGAT 
GAAGGCAATG GCTTCGACAC CTACAACATA CCCAACTTTG GACCGACAAT TTATTGTGGT 
TTATTCGGCA TTACGTGGGT GCTGCGTCAA ATAGCCACAG ACAATGACCT GGGCCATCCA 
TTGTGTACCA ACTTGCGAAA TGGCAACTGG TTGATTGAAT ACGTTTTCAA ACGCCTTAAA 
GCCGATGAGG GGACCCGCGC TTTGGGGGAA TGGATTGAAG ACTACAGTAA GCCCCTCCTA 
AATATGCCCC GTTACTTAGT TCCCTGTTAT TTCGATGTCT TCATTGGGCA AATATACCCT 
CAGCTACTAA ATGCTGGCTA TCAATTGATG TCTAATTTTA TCAAGCAGGG ATCGCCTTTT 
GTTCAGTCGT TGGCTTTGGG GTCGTTGCAA TTTGGCGGTT ACCTCAGATC TGCCTGTCTG 
CCAGATATGT CGCCCAATCT GGCTCCGCCA AAACCTCCTA GCAGAAAAAA CGAAAACGGT 
GAAACAGTCC AGGCCTGCGT TACTTTATCA GCAGGATTGC CCCACTTCGC CGTGGGTTAC 
ATGCGAAATT GGGGTAGAGA CACCTTTATT GCTCTGCGCG GGCTGTTCAT CTTAACTGGC 
CGGTTTGAAG AAGCCCGACA ACATATTCTG GGTTACGGGT CGTGTTTGAG GCACGGCCTC 
ATTCCGAATT TACTGGATGC TGGTAGAAAT CCCAGGTTTA ACTGCCGAGA TGCCATTTGG 
TGGTGGCTCT ATTCGCTCAA ATCATATTGC GAAGAATCCC CTAAGGGCGT TGCTATTCTT 
GCCGATGTTG TATCACGCAT ATTTCCTTCG GATGATTCTT CTCCACAACC GCCTGGTGCT 
GTGGATCAAC CGCTATACAA TGTAATTCAG GAATCGTTAA CTGTCCATTT CCAAGGCCTA 
CAATTTCGCG AAAGGAACGC TGGACGAGAA ATTGATGAAC ACATGACCGA TGCAGGATTT 
AACAACCAAA TCGGAACTCA TCCGGAAACT GGATTCGTTT TTGGTGGGAA TGAATGGAAT 
TGCGGTACTT GGATGGACAA AATGGGTTCA TCAGACAAGG CTGGCAATCG GGGCAAACCG 
GCGTCGCCTC GTGACGGATC TGCTGTCGAA CTCGTAGGCT TATCTAAAGC CTGTATCACT 
TGGTTAATGC ACTTAAATGA TGAAGGTCAG TACCCTTATG ATGGGGTTCA GCGAACACAT 
AAGAATGGAA CGGTGACCAA ATGGACCTTC AGAGAATGGT CGGACAAAAT TCAACAAAAC 
TTCGAAAAGA AATTCTGGAT TTCAGGGAAC CCGAACCCAG AAGAATGTAG GCCTGATTTA 
GTGAATAAGA AGAACATTTA CAAAGACAGC TTCGGAGCGT CTCAGCCATG GACAGATTTT 
CAGCTTAGGT GTAATTTTCC CATTGCCATG GTCGCTGCTC CCGAGTTGTT TAATCCTCAA 
CGTGCATGGG CCGCATTAGA GCAAGTCGAA AAGTATCTCT TGGGGCCACT AGGCATGAAA 
ACGCTAGACC CCGAAGACTG GAATTACCGG GGCGACTATG ATAATTCGAT TGATTCAGAT 
GATTTACTTA CGGCCCATGG CTTCAATTAT CATCAAGGCC CTGAATGGGT TTGGCCTGTG 
GGTTTTTTCC TAAGAGCAAA GTTAATATTT GCTGCTCAGA ATGGACTGTT AGACGAAACA 
GTGACCAAAA CAAAACAAAT CTTATCAAAG CATTTTATCG AAGTGGAGAC GCCACCGTGG 
CGTGGATTAC CGGAATTAAC CAACAGCGAT GGATCCTACT GCCCGGACAG TGCGAGAACC 
CAAGCTTGGA GCATGTCCTG CATTCTCGAG GTCCTGTATG ACTTGGAGAA AATCGAATCC 
AATAGAAGTG TTGCAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109539064 ( XM_019906637.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019762196.1 Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X6, mRNA.

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CloneID	ODe42981
Clone ID Related Accession (Same CDS sequence)	XM_019906637.1
Accession Version	XM_019906637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	glycogen debranching enzyme isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTG TAAATATATT GATACTGAAC GATAAAGCAA ACTTGGATTC CGAGCTTCAC 
AGAATTCAAA AAAATGTTAA ATTGGAGTTC AGACTCGGCC CTTCTTTGCT GGGCAGGAAT 
ATAAAGCTGT ATTGCAACTA TCCGAAGTCA ACTAACGGAA TTGTGGAAGA ATTCAGCAGA 
GATTGTTATA CGTTAATAAG ATGGTCACGG AATGAAGGAT GCAAAAACGC AGACGGTAGC 
TCAACCTTTG CACAGATCAC CCCCACGATC GCAGGAAGCT TCCACTACTA CTTCGTTGAA 
GACGATGAGT TTACTAAGAC GAATTTCAAA AACGGATCCG GCTATTTTAT AGTTGACCCT 
GTATTGAAAT ATGGTGCCAA TGAGCTGTTG CCTCTGAACT GTATACAGAG TCAAACGGTA 
TTGTCAAAAC TTTTGGGGCC GTTCTCCACA TGGGAAAACA AGATACGGGT TGCCAAAGAA 
TCCGGTTATA ACATGGTGCA TTTTACGCCC ATTCAGCAAC TAGGAGGATC CCATTCCGCA 
TACAGTGTCC AAGACCACTT GAAATTAAAT GTTTCTTTTA ACGAAGAAAA TGGACGAGAA 
CCGTCATTTG AAGATATCGA GACGCTGACC GCAAAAATGC GTGAAGATTG GAAGATGTTG 
TCCGTATGTG ACATAGTGAT AAATCACACA GCCAATGAAA GTGATTGGAT TAAAGAACAC 
CCTGAAGCAG CATACAATTG CTTGAACTGT CCTTACATGC GACCAGCCTA TCTGTTAGAT 
GAAGCTCTCC ATCAGTTTTC CATGGATATA AGAAAAGGCC TCTATGAGGA CCGGGGAATC 
CCTCTTGACG TTTCAACGGA AGATCATCTA AACGCAATCC GTTATCACTT TAAACAAAAC 
GTGTTACAAC CATTGAAAAT TCATGAGCTG TTTATTGTAG ACGTAACCCA ATACTTGGAA 
CAGTTTTTAA CACAGGCCAG AAAAACCGCA CCTCTCAATG ACTGCGAAAG AAACCTCTTA 
CCGGACTTGA AAATTATTCA AGATGTTCAG TTTAGGAGAT TGAGCAGTTC GGTCGACTTA 
AATTTGGCCC TCGCCAAGTT CAACTGCTAT CGGCCAGATT GTTACGATGA GGAGTCGAGA 
GTTAGAAGAT GTTCCGAGGA TTTTAAAATT CAGCTGGATG CCCTCAATCA GGCTGCAATT 
GATGAAGTCA CTGCTGACCT TAGTGCTGCA GTTGAAAACG TTATTGCTGG CATTCGGTAT 
TTCAGAGTAC AGCCAGACGG CCCTCAATAT AAGGCCATTA CCATTGATCA TCCATTGGTG 
TACAGGTACT TTACCGATTA TCATTCACCC AAATCCCTGG CAGAAGCTGA AGAAACAATG 
TACAGTACAA ACGGACGGTT TCTCATGGCC CATAATGGTT GGGTGATGAG CGCTGAGCCA 
TTAAAGAACT TTGCCGATGC CGGCACCAAG ATTTACATTA GAAGGGAACT TATCGCTTGG 
GGCGACAGCG TTAAGCTACG CTTCGGGGAC AAGCCGGCCG ATTCTCCATT TTTGTGGAAT 
TACATGCGGC AGTACGTAGA ACAGACGGTT AAAATATTTG ATGGTATTCG GCTGGACAAC 
TGCCACTCAA CTTCCCTTCC CGTTGCCGAA TATCTTTTGG ACTGCGCCAG AAGAATCCGT 
CCTGATCTTT ATGTGGTGGC CGAGTTGTTC ACCAACTCGG CCTTGACTGA CAACATTTTC 
GTAAATCGTT TGGGCATCAC GTCGCTTATT CGAGAAGCGA TGTCCGCATG GGATGCTCAT 
GAACAGGGAC GTCTGGTATA TCGATTCGGA GGTGTTCCAG TGGGTGCTTT TTACAAACCT 
TCAAAAGTAC CATTGGTCCC TATGGTGGCA CATGCACTTT TCCTCGATCA AACCCATGAT 
AATCCCAGTC CAGTCGAAAA AAGATCTGTT TTCGATTTGC TGCCGAGCGC CGGTTTAGTA 
AACATGGCAT GTTGTGCCAC CGGCTCAAAT CGAGGCTACG ACGAACTGGT TCCCCACCAC 
ATCCACGTAG TGGATGAAAC TAGACAATAC GCTGAGTGGA GTCATCATGA AAAGCTTCCT 
TCCAAAACCA GATATGTTAA TAAGTGGTCA GGCATTATTG CGGCCAAAAG AGCTCTGCAC 
GAGTTGCACT TTAAGCTAGG AATGGAAGGA TTCGACCAGG TATTTGTGAA TCAAGTAGAT 
GGCGATATTG TTGAGGTGAC CAGGCACCAA CCGGGCACCC ACACCAGTTA TGTCCTAGTA 
GCATTCACGG CCTTTGAACA TCCCACCGGT GACCACCAGA GAGCCATTAA ACCGCTTTCT 
GTTGAAGGAG TTCTGGATGA AATTGTACTG GAAGCCTACT TAATTCATGC AGATTCCACT 
TCCAGGTTTG GCAGATTTGA AAAATTTGAG AAAGACCCCG ATTGGATCAA CGGATTAGCC 
GAATATAAGC TTTCAATTAA AGAGCACATC CAGTTGCGCG AATCTGAGTT TTTCGAGGAA 
GCAGCTGACA GTAGTTCTAA TTTCGTCCGG TTAAACTATA AAAACTTCAA GCCCGGATCA 
ATTGCAGTGT TGAAATTTTC TTTGCCCCAA GGAATCCGAG ATGCGATATC TCTGTTGAAA 
CCCCCACAGG CTCCAAATCT AGAGAAAATT GTAAGTGAAT TGACTCTAGC AGATTTGAAC 
AGAACTCTCT TTCGATGTGG CGAAGAAGAA AGGGATGAAG GCAATGGCTT CGACACCTAC 
AACATACCCA ACTTTGGACC GACAATTTAT TGTGGTTTAT TCGGCATTAC GTGGGTGCTG 
CGTCAAATAG CCACAGACAA TGACCTGGGC CATCCATTGT GTACCAACTT GCGAAATGGC 
AACTGGTTGA TTGAATACGT TTTCAAACGC CTTAAAGCCG ATGAGGGGAC CCGCGCTTTG 
GGGGAATGGA TTGAAGACTA CAGTAAGCCC CTCCTAAATA TGCCCCGTTA CTTAGTTCCC 
TGTTATTTCG ATGTCTTCAT TGGGCAAATA TACCCTCAGC TACTAAATGC TGGCTATCAA 
TTGATGTCTA ATTTTATCAA GCAGGGATCG CCTTTTGTTC AGTCGTTGGC TTTGGGGTCG 
TTGCAATTTG GCGGTTACCT CAGATCTGCC TGTCTGCCAG ATATGTCGCC CAATCTGGCT 
CCGCCAAAAC CTCCTAGCAG AAAAAACGAA AACGGTGAAA CAGTCCAGGC CTGCGTTACT 
TTATCAGCAG GATTGCCCCA CTTCGCCGTG GGTTACATGC GAAATTGGGG TAGAGACACC 
TTTATTGCTC TGCGCGGGCT GTTCATCTTA ACTGGCCGGT TTGAAGAAGC CCGACAACAT 
ATTCTGGGTT ACGGGTCGTG TTTGAGGCAC GGCCTCATTC CGAATTTACT GGATGCTGGT 
AGAAATCCCA GGTTTAACTG CCGAGATGCC ATTTGGTGGT GGCTCTATTC GCTCAAATCA 
TATTGCGAAG AATCCCCTAA GGGCGTTGCT ATTCTTGCCG ATGTTGTATC ACGCATATTT 
CCTTCGGATG ATTCTTCTCC ACAACCGCCT GGTGCTGTGG ATCAACCGCT ATACAATGTA 
ATTCAGGAAT CGTTAACTGT CCATTTCCAA GGCCTACAAT TTCGCGAAAG GAACGCTGGA 
CGAGAAATTG ATGAACACAT GACCGATGCA GGATTTAACA ACCAAATCGG AACTCATCCG 
GAAACTGGAT TCGTTTTTGG TGGGAATGAA TGGAATTGCG GTACTTGGAT GGACAAAATG 
GGTTCATCAG ACAAGGCTGG CAATCGGGGC AAACCGGCGT CGCCTCGTGA CGGATCTGCT 
GTCGAACTCG TAGGCTTATC TAAAGCCTGT ATCACTTGGT TAATGCACTT AAATGATGAA 
GGTCAGTACC CTTATGATGG GGTTCAGCGA ACACATAAGA ATGGAACGGT GACCAAATGG 
ACCTTCAGAG AATGGTCGGA CAAAATTCAA CAAAACTTCG AAAAGAAATT CTGGATTTCA 
GGGAACCCGA ACCCAGAAGA ATGTAGGCCT GATTTAGTGA ATAAGAAGAA CATTTACAAA 
GACAGCTTCG GAGCGTCTCA GCCATGGACA GATTTTCAGC TTAGGTGTAA TTTTCCCATT 
GCCATGGTCG CTGCTCCCGA GTTGTTTAAT CCTCAACGTG CATGGGCCGC ATTAGAGCAA 
GTCGAAAAGT ATCTCTTGGG GCCACTAGGC ATGAAAACGC TAGACCCCGA AGACTGGAAT 
TACCGGGGCG ACTATGATAA TTCGATTGAT TCAGATGATT TACTTACGGC CCATGGCTTC 
AATTATCATC AAGGCCCTGA ATGGGTTTGG CCTGTGGGTT TTTTCCTAAG AGCAAAGTTA 
ATATTTGCTG CTCAGAATGG ACTGTTAGAC GAAACAGTGA CCAAAACAAA ACAAATCTTA 
TCAAAGCATT TTATCGAAGT GGAGACGCCA CCGTGGCGTG GATTACCGGA ATTAACCAAC 
AGCGATGGAT CCTACTGCCC GGACAGTGCG AGAACCCAAG CTTGGAGCAT GTCCTGCATT 
CTCGAGGTCC TGTATGACTT GGAGAAAATC GAATCCAATA GAAGTGTTGC AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019762196.1
CDS	569..5185
Translation	MASVNILILNDKANLDSELHRIQKNVKLEFRLGPSLLGRNIKLYCNYPKSTNGIVEEFSRDCYTLIRWSRNEGCKNADGSSTFAQITPTIAGSFHYYFVEDDEFTKTNFKNGSGYFIVDPVLKYGANELLPLNCIQSQTVLSKLLGPFSTWENKIRVAKESGYNMVHFTPIQQLGGSHSAYSVQDHLKLNVSFNEENGREPSFEDIETLTAKMREDWKMLSVCDIVINHTANESDWIKEHPEAAYNCLNCPYMRPAYLLDEALHQFSMDIRKGLYEDRGIPLDVSTEDHLNAIRYHFKQNVLQPLKIHELFIVDVTQYLEQFLTQARKTAPLNDCERNLLPDLKIIQDVQFRRLSSSVDLNLALAKFNCYRPDCYDEESRVRRCSEDFKIQLDALNQAAIDEVTADLSAAVENVIAGIRYFRVQPDGPQYKAITIDHPLVYRYFTDYHSPKSLAEAEETMYSTNGRFLMAHNGWVMSAEPLKNFADAGTKIYIRRELIAWGDSVKLRFGDKPADSPFLWNYMRQYVEQTVKIFDGIRLDNCHSTSLPVAEYLLDCARRIRPDLYVVAELFTNSALTDNIFVNRLGITSLIREAMSAWDAHEQGRLVYRFGGVPVGAFYKPSKVPLVPMVAHALFLDQTHDNPSPVEKRSVFDLLPSAGLVNMACCATGSNRGYDELVPHHIHVVDETRQYAEWSHHEKLPSKTRYVNKWSGIIAAKRALHELHFKLGMEGFDQVFVNQVDGDIVEVTRHQPGTHTSYVLVAFTAFEHPTGDHQRAIKPLSVEGVLDEIVLEAYLIHADSTSRFGRFEKFEKDPDWINGLAEYKLSIKEHIQLRESEFFEEAADSSSNFVRLNYKNFKPGSIAVLKFSLPQGIRDAISLLKPPQAPNLEKIVSELTLADLNRTLFRCGEEERDEGNGFDTYNIPNFGPTIYCGLFGITWVLRQIATDNDLGHPLCTNLRNGNWLIEYVFKRLKADEGTRALGEWIEDYSKPLLNMPRYLVPCYFDVFIGQIYPQLLNAGYQLMSNFIKQGSPFVQSLALGSLQFGGYLRSACLPDMSPNLAPPKPPSRKNENGETVQACVTLSAGLPHFAVGYMRNWGRDTFIALRGLFILTGRFEEARQHILGYGSCLRHGLIPNLLDAGRNPRFNCRDAIWWWLYSLKSYCEESPKGVAILADVVSRIFPSDDSSPQPPGAVDQPLYNVIQESLTVHFQGLQFRERNAGREIDEHMTDAGFNNQIGTHPETGFVFGGNEWNCGTWMDKMGSSDKAGNRGKPASPRDGSAVELVGLSKACITWLMHLNDEGQYPYDGVQRTHKNGTVTKWTFREWSDKIQQNFEKKFWISGNPNPEECRPDLVNKKNIYKDSFGASQPWTDFQLRCNFPIAMVAAPELFNPQRAWAALEQVEKYLLGPLGMKTLDPEDWNYRGDYDNSIDSDDLLTAHGFNYHQGPEWVWPVGFFLRAKLIFAAQNGLLDETVTKTKQILSKHFIEVETPPWRGLPELTNSDGSYCPDSARTQAWSMSCILEVLYDLEKIESNRSVAN

Target ORF information:

RefSeq Version	XM_019906637.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae glycogen debranching enzyme (LOC109539064), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019906637.1

ORF Insert Sequence:

ATGGCATCTG TAAATATATT GATACTGAAC GATAAAGCAA ACTTGGATTC CGAGCTTCAC 
AGAATTCAAA AAAATGTTAA ATTGGAGTTC AGACTCGGCC CTTCTTTGCT GGGCAGGAAT 
ATAAAGCTGT ATTGCAACTA TCCGAAGTCA ACTAACGGAA TTGTGGAAGA ATTCAGCAGA 
GATTGTTATA CGTTAATAAG ATGGTCACGG AATGAAGGAT GCAAAAACGC AGACGGTAGC 
TCAACCTTTG CACAGATCAC CCCCACGATC GCAGGAAGCT TCCACTACTA CTTCGTTGAA 
GACGATGAGT TTACTAAGAC GAATTTCAAA AACGGATCCG GCTATTTTAT AGTTGACCCT 
GTATTGAAAT ATGGTGCCAA TGAGCTGTTG CCTCTGAACT GTATACAGAG TCAAACGGTA 
TTGTCAAAAC TTTTGGGGCC GTTCTCCACA TGGGAAAACA AGATACGGGT TGCCAAAGAA 
TCCGGTTATA ACATGGTGCA TTTTACGCCC ATTCAGCAAC TAGGAGGATC CCATTCCGCA 
TACAGTGTCC AAGACCACTT GAAATTAAAT GTTTCTTTTA ACGAAGAAAA TGGACGAGAA 
CCGTCATTTG AAGATATCGA GACGCTGACC GCAAAAATGC GTGAAGATTG GAAGATGTTG 
TCCGTATGTG ACATAGTGAT AAATCACACA GCCAATGAAA GTGATTGGAT TAAAGAACAC 
CCTGAAGCAG CATACAATTG CTTGAACTGT CCTTACATGC GACCAGCCTA TCTGTTAGAT 
GAAGCTCTCC ATCAGTTTTC CATGGATATA AGAAAAGGCC TCTATGAGGA CCGGGGAATC 
CCTCTTGACG TTTCAACGGA AGATCATCTA AACGCAATCC GTTATCACTT TAAACAAAAC 
GTGTTACAAC CATTGAAAAT TCATGAGCTG TTTATTGTAG ACGTAACCCA ATACTTGGAA 
CAGTTTTTAA CACAGGCCAG AAAAACCGCA CCTCTCAATG ACTGCGAAAG AAACCTCTTA 
CCGGACTTGA AAATTATTCA AGATGTTCAG TTTAGGAGAT TGAGCAGTTC GGTCGACTTA 
AATTTGGCCC TCGCCAAGTT CAACTGCTAT CGGCCAGATT GTTACGATGA GGAGTCGAGA 
GTTAGAAGAT GTTCCGAGGA TTTTAAAATT CAGCTGGATG CCCTCAATCA GGCTGCAATT 
GATGAAGTCA CTGCTGACCT TAGTGCTGCA GTTGAAAACG TTATTGCTGG CATTCGGTAT 
TTCAGAGTAC AGCCAGACGG CCCTCAATAT AAGGCCATTA CCATTGATCA TCCATTGGTG 
TACAGGTACT TTACCGATTA TCATTCACCC AAATCCCTGG CAGAAGCTGA AGAAACAATG 
TACAGTACAA ACGGACGGTT TCTCATGGCC CATAATGGTT GGGTGATGAG CGCTGAGCCA 
TTAAAGAACT TTGCCGATGC CGGCACCAAG ATTTACATTA GAAGGGAACT TATCGCTTGG 
GGCGACAGCG TTAAGCTACG CTTCGGGGAC AAGCCGGCCG ATTCTCCATT TTTGTGGAAT 
TACATGCGGC AGTACGTAGA ACAGACGGTT AAAATATTTG ATGGTATTCG GCTGGACAAC 
TGCCACTCAA CTTCCCTTCC CGTTGCCGAA TATCTTTTGG ACTGCGCCAG AAGAATCCGT 
CCTGATCTTT ATGTGGTGGC CGAGTTGTTC ACCAACTCGG CCTTGACTGA CAACATTTTC 
GTAAATCGTT TGGGCATCAC GTCGCTTATT CGAGAAGCGA TGTCCGCATG GGATGCTCAT 
GAACAGGGAC GTCTGGTATA TCGATTCGGA GGTGTTCCAG TGGGTGCTTT TTACAAACCT 
TCAAAAGTAC CATTGGTCCC TATGGTGGCA CATGCACTTT TCCTCGATCA AACCCATGAT 
AATCCCAGTC CAGTCGAAAA AAGATCTGTT TTCGATTTGC TGCCGAGCGC CGGTTTAGTA 
AACATGGCAT GTTGTGCCAC CGGCTCAAAT CGAGGCTACG ACGAACTGGT TCCCCACCAC 
ATCCACGTAG TGGATGAAAC TAGACAATAC GCTGAGTGGA GTCATCATGA AAAGCTTCCT 
TCCAAAACCA GATATGTTAA TAAGTGGTCA GGCATTATTG CGGCCAAAAG AGCTCTGCAC 
GAGTTGCACT TTAAGCTAGG AATGGAAGGA TTCGACCAGG TATTTGTGAA TCAAGTAGAT 
GGCGATATTG TTGAGGTGAC CAGGCACCAA CCGGGCACCC ACACCAGTTA TGTCCTAGTA 
GCATTCACGG CCTTTGAACA TCCCACCGGT GACCACCAGA GAGCCATTAA ACCGCTTTCT 
GTTGAAGGAG TTCTGGATGA AATTGTACTG GAAGCCTACT TAATTCATGC AGATTCCACT 
TCCAGGTTTG GCAGATTTGA AAAATTTGAG AAAGACCCCG ATTGGATCAA CGGATTAGCC 
GAATATAAGC TTTCAATTAA AGAGCACATC CAGTTGCGCG AATCTGAGTT TTTCGAGGAA 
GCAGCTGACA GTAGTTCTAA TTTCGTCCGG TTAAACTATA AAAACTTCAA GCCCGGATCA 
ATTGCAGTGT TGAAATTTTC TTTGCCCCAA GGAATCCGAG ATGCGATATC TCTGTTGAAA 
CCCCCACAGG CTCCAAATCT AGAGAAAATT GTAAGTGAAT TGACTCTAGC AGATTTGAAC 
AGAACTCTCT TTCGATGTGG CGAAGAAGAA AGGGATGAAG GCAATGGCTT CGACACCTAC 
AACATACCCA ACTTTGGACC GACAATTTAT TGTGGTTTAT TCGGCATTAC GTGGGTGCTG 
CGTCAAATAG CCACAGACAA TGACCTGGGC CATCCATTGT GTACCAACTT GCGAAATGGC 
AACTGGTTGA TTGAATACGT TTTCAAACGC CTTAAAGCCG ATGAGGGGAC CCGCGCTTTG 
GGGGAATGGA TTGAAGACTA CAGTAAGCCC CTCCTAAATA TGCCCCGTTA CTTAGTTCCC 
TGTTATTTCG ATGTCTTCAT TGGGCAAATA TACCCTCAGC TACTAAATGC TGGCTATCAA 
TTGATGTCTA ATTTTATCAA GCAGGGATCG CCTTTTGTTC AGTCGTTGGC TTTGGGGTCG 
TTGCAATTTG GCGGTTACCT CAGATCTGCC TGTCTGCCAG ATATGTCGCC CAATCTGGCT 
CCGCCAAAAC CTCCTAGCAG AAAAAACGAA AACGGTGAAA CAGTCCAGGC CTGCGTTACT 
TTATCAGCAG GATTGCCCCA CTTCGCCGTG GGTTACATGC GAAATTGGGG TAGAGACACC 
TTTATTGCTC TGCGCGGGCT GTTCATCTTA ACTGGCCGGT TTGAAGAAGC CCGACAACAT 
ATTCTGGGTT ACGGGTCGTG TTTGAGGCAC GGCCTCATTC CGAATTTACT GGATGCTGGT 
AGAAATCCCA GGTTTAACTG CCGAGATGCC ATTTGGTGGT GGCTCTATTC GCTCAAATCA 
TATTGCGAAG AATCCCCTAA GGGCGTTGCT ATTCTTGCCG ATGTTGTATC ACGCATATTT 
CCTTCGGATG ATTCTTCTCC ACAACCGCCT GGTGCTGTGG ATCAACCGCT ATACAATGTA 
ATTCAGGAAT CGTTAACTGT CCATTTCCAA GGCCTACAAT TTCGCGAAAG GAACGCTGGA 
CGAGAAATTG ATGAACACAT GACCGATGCA GGATTTAACA ACCAAATCGG AACTCATCCG 
GAAACTGGAT TCGTTTTTGG TGGGAATGAA TGGAATTGCG GTACTTGGAT GGACAAAATG 
GGTTCATCAG ACAAGGCTGG CAATCGGGGC AAACCGGCGT CGCCTCGTGA CGGATCTGCT 
GTCGAACTCG TAGGCTTATC TAAAGCCTGT ATCACTTGGT TAATGCACTT AAATGATGAA 
GGTCAGTACC CTTATGATGG GGTTCAGCGA ACACATAAGA ATGGAACGGT GACCAAATGG 
ACCTTCAGAG AATGGTCGGA CAAAATTCAA CAAAACTTCG AAAAGAAATT CTGGATTTCA 
GGGAACCCGA ACCCAGAAGA ATGTAGGCCT GATTTAGTGA ATAAGAAGAA CATTTACAAA 
GACAGCTTCG GAGCGTCTCA GCCATGGACA GATTTTCAGC TTAGGTGTAA TTTTCCCATT 
GCCATGGTCG CTGCTCCCGA GTTGTTTAAT CCTCAACGTG CATGGGCCGC ATTAGAGCAA 
GTCGAAAAGT ATCTCTTGGG GCCACTAGGC ATGAAAACGC TAGACCCCGA AGACTGGAAT 
TACCGGGGCG ACTATGATAA TTCGATTGAT TCAGATGATT TACTTACGGC CCATGGCTTC 
AATTATCATC AAGGCCCTGA ATGGGTTTGG CCTGTGGGTT TTTTCCTAAG AGCAAAGTTA 
ATATTTGCTG CTCAGAATGG ACTGTTAGAC GAAACAGTGA CCAAAACAAA ACAAATCTTA 
TCAAAGCATT TTATCGAAGT GGAGACGCCA CCGTGGCGTG GATTACCGGA ATTAACCAAC 
AGCGATGGAT CCTACTGCCC GGACAGTGCG AGAACCCAAG CTTGGAGCAT GTCCTGCATT 
CTCGAGGTCC TGTATGACTT GGAGAAAATC GAATCCAATA GAAGTGTTGC AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC109539064 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム