cldne cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cldne
Entrez Gene ID	81584
Full Name	claudin e
Synonyms	cb84,fb16e12,wu:fb16e12
General protein information	Preferred Names claudin e Names claudin-4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131765.1	NP_571840.1	claudin-4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLDN6	XP_523276.1
	CLDN6	XP_001088715.1
Mus musculus (house mouse)	Cldn6	NP_061247.1
Xenopus tropicalis (tropical clawed frog)	cldn6.1	NP_988873.1
Rattus norvegicus (Norway rat)	Cldn6	NP_001095834.1
Bos taurus (cattle)	CLDN6	NP_001192626.1
Homo sapiens (human)	CLDN6	NP_067018.2
Canis lupus familiaris (dog)	CLDN6	XP_547168.2
Danio rerio (zebrafish)	cldne	NP_571840.1
Danio rerio (zebrafish)	cldnd	NP_851295.1
Xenopus tropicalis (tropical clawed frog)	LOC100493413	XP_002941739.1

Gene Ontology
Bibliography

Related articles in PubMed

Rapid functional analysis of computationally complex rare human IRF6 gene variants using a novel zebrafish model.
Li EB, Truong D, Hallett SA, Mukherjee K, Schutte BC, Liao EC
PLoS genetics13(9)e1007009(2017 Sep)

Proliferation-independent regulation of organ size by Fgf/Notch signaling.
Kozlovskaja-Gumbrien? A, Yi R, Alexander R, Aman A, Jiskra R, Nagelberg D, Knaut H, McClain M, Piotrowski T
eLife6(2017 Jan)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Irf6 directly regulates Klf17 in zebrafish periderm and Klf4 in murine oral epithelium, and dominant-negative KLF4 variants are present in patients with cleft lip and palate.
Liu H, Leslie EJ, Jia Z, Smith T, Eshete M, Butali A, Dunnwald M, Murray J, Cornell RA
Human molecular genetics25(4)766-76(2016 Feb)

The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models.
Shu Y, Lou Q, Dai Z, Dai X, He J, Hu W, Yin Z
Scientific reports618597(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

important for zebrafish epiboly
Title: The tight junction component Claudin E is required for zebrafish epiboly.

The following cldne gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cldne cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00799	NM_131765.1 Latest version!	Danio rerio claudin e (cldne), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cldne ( NM_131765.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571840.1 Danio rerio claudin e (cldne), mRNA.

Price & Availability↑

CloneID	ODa00799
Clone ID Related Accession (Same CDS sequence)	NM_131765.1
Accession Version	NM_131765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-26
Organism	Danio rerio(zebrafish)
Product	claudin-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF359425.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF359425.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGTCTA TGTGCCGAGA GATCCTGGGC ATGTGCCTGG CCATCATTGG CTTCTTGGGA 
GCCATTATTA TTTGTGCTTT GCCCATGTGG AAGGTGACCG CGTTCATCGG AGCCAACATC 
GTGACGGCGC AGACCATCTG GGAGGGTTTG TGGATGAACT GTGTGATGCA GAGCACGGGA 
CAGATGCAGT GTAAGATCTA CGACTCGCTC CTGGCCTTGC CTCAGGACCT GCAGGCCGCT 
CGAGCTCTCG TGGTCATCGC CATCATCGTC AGCTTTTTCG CGCTCATCCT GGGCATCGCC 
GGCGGGAAAT GCACCAACTT CGTGGAAAGG GAAGACGCCA AGGCTAAAGT GTCCATCGCC 
AGCGGCGTGA TCTTCATCAT TGCTGGAGTT CTGGTGCTGG TGCCTGTTTG CTGGTCCGCC 
AACACCATCA TCAGAGATTT CTACAATCCT CTGCTCACGG ATGCTCAGCG GAGGGAGATG 
GGGGCTTCGC TGTACATCGG ATGGGGCGTC GCAGCGCTGC TGATCATCGG AGGTGGAATC 
CTGTGCAGCT CCTGCCCTCC AAAAGATGAA AAATACAACA TGAAATACTC CCAGCCGCGC 
TCCACCGCCA CCAGCAGAGC CTACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571840.1
CDS	49..678
Translation	MVSMCREILGMCLAIIGFLGAIIICALPMWKVTAFIGANIVTAQTIWEGLWMNCVMQSTGQMQCKIYDSLLALPQDLQAARALVVIAIIVSFFALILGIAGGKCTNFVEREDAKAKVSIASGVIFIIAGVLVLVPVCWSANTIIRDFYNPLLTDAQRREMGASLYIGWGVAALLIIGGGILCSSCPPKDEKYNMKYSQPRSTATSRAYV

Target ORF information:

RefSeq Version	NM_131765.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio claudin e (cldne), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131765.1

ORF Insert Sequence:

ATGGTGTCTA TGTGCCGAGA GATCCTGGGC ATGTGCCTGG CCATCATTGG CTTCTTGGGA 
GCCATTATTA TTTGTGCTTT GCCCATGTGG AAGGTGACCG CGTTCATCGG AGCCAACATC 
GTGACGGCGC AGACCATCTG GGAGGGTTTG TGGATGAACT GTGTGATGCA GAGCACGGGA 
CAGATGCAGT GTAAGATCTA CGACTCGCTC CTGGCCTTGC CTCAGGACCT GCAGGCCGCT 
CGAGCTCTCG TGGTCATCGC CATCATCGTC AGCTTTTTCG CGCTCATCCT GGGCATCGCC 
GGCGGGAAAT GCACCAACTT CGTGGAAAGG GAAGACGCCA AGGCTAAAGT GTCCATCGCC 
AGCGGCGTGA TCTTCATCAT TGCTGGAGTT CTGGTGCTGG TGCCTGTTTG CTGGTCCGCC 
AACACCATCA TCAGAGATTT CTACAATCCT CTGCTCACGG ATGCTCAGCG GAGGGAGATG 
GGGGCTTCGC TGTACATCGG ATGGGGCGTC GCAGCGCTGC TGATCATCGG AGGTGGAATC 
CTGTGCAGCT CCTGCCCTCC AAAAGATGAA AAATACAACA TGAAATACTC CCAGCCGCGC 
TCCACCGCCA CCAGCAGAGC CTACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rapid functional analysis of computationally complex rare human IRF6 gene variants using a novel zebrafish model.
PLoS genetics13(9)e1007009(2017 Sep)
Li EB,Truong D,Hallett SA,Mukherjee K,Schutte BC,Liao EC

Proliferation-independent regulation of organ size by Fgf/Notch signaling.
eLife6(2017 Jan)
Kozlovskaja-Gumbrien? A,Yi R,Alexander R,Aman A,Jiskra R,Nagelberg D,Knaut H,McClain M,Piotrowski T

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Irf6 directly regulates Klf17 in zebrafish periderm and Klf4 in murine oral epithelium, and dominant-negative KLF4 variants are present in patients with cleft lip and palate.
Human molecular genetics25(4)766-76(2016 Feb)
Liu H,Leslie EJ,Jia Z,Smith T,Eshete M,Butali A,Dunnwald M,Murray J,Cornell RA

The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models.
Scientific reports618597(2016 Jan)
Shu Y,Lou Q,Dai Z,Dai X,He J,Hu W,Yin Z