manf cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	manf
Entrez Gene ID	767691
Full Name	mesencephalic astrocyte-derived neurotrophic factor
Synonyms	im:6909481,zgc:153327
General protein information	Preferred Names mesencephalic astrocyte-derived neurotrophic factor Names mesencephalic astrocyte-derived neurotrophic factor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001076629.1	NP_001070097.1	mesencephalic astrocyte-derived neurotrophic factor precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	Y54G2A.23	NP_500273.2
Bos taurus (cattle)	MANF	NP_001094681.1
Drosophila melanogaster (fruit fly)	Manf	NP_477445.1
Homo sapiens (human)	MANF	NP_006001.3
Mus musculus (house mouse)	Manf	NP_083379.2
	LOC700659	XP_002802839.1
Danio rerio (zebrafish)	manf	NP_001070097.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003016	XP_311862.3
Pan troglodytes (chimpanzee)	MANF	XP_001169644.3
Canis lupus familiaris (dog)	MANF	XP_003639808.2
Xenopus tropicalis (tropical clawed frog)	manf	NP_001016425.1

Gene Ontology
Bibliography

Related articles in PubMed

Enhanced uptake of BPA in the presence of nanoplastics can lead to neurotoxic effects in adult zebrafish.
Chen Q, Yin D, Jia Y, Schiwy S, Legradi J, Yang S, Hollert H
The Science of the total environment6091312-1321(2017 Dec)

Parental transfer of microcystin-LR induced transgenerational effects of developmental neurotoxicity in zebrafish offspring.
Wu Q, Yan W, Cheng H, Liu C, Hung TC, Guo X, Li G
Environmental pollution (Barking, Essex : 1987)231(Pt 1)471-478(2017 Dec)

A Mixture of Persistent Organic Pollutants and Perfluorooctanesulfonic Acid Induces Similar Behavioural Responses, but Different Gene Expression Profiles in Zebrafish Larvae.
Khezri A, Fraser TW, Nourizadeh-Lillabadi R, Kamstra JH, Berg V, Zimmer KE, Ropstad E
International journal of molecular sciences18(2)(2017 Jan)

A Novel Developmental Role for Dopaminergic Signaling to Specify Hypothalamic Neurotransmitter Identity.
Chen YC, Semenova S, Rozov S, Sundvik M, Bonkowsky JL, Panula P
The Journal of biological chemistry291(42)21880-21892(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that MANF is involved in the regulation of the development of dopaminergic system in zebrafish.
Title: MANF regulates dopaminergic neuron development in larval zebrafish.

The following manf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the manf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35303	NM_001076629.1 Latest version!	Danio rerio mesencephalic astrocyte-derived neurotrophic factor (manf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

manf ( NM_001076629.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070097.1 Danio rerio mesencephalic astrocyte-derived neurotrophic factor (manf), mRNA.

Price & Availability↑

CloneID	ODa35303
Clone ID Related Accession (Same CDS sequence)	NM_001076629.1
Accession Version	NM_001076629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Danio rerio(zebrafish)
Product	mesencephalic astrocyte-derived neurotrophic factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124316.1. ##Evidence-Data-START## Transcript exon combination :: BC124316.1, EH503442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTATATT TAAGTGGACT TTCTGTGGCA TTTGCTCTCG CGCTGGTTCC GAGCTGCAGT 
GATGCTTTGA AAGATGGAGA GTGTGAAGTC TGTGTGGGTT TCCTCCAGCG GCTTTACCAG 
ACGATACAAG AAAACAACGT CAAGTTTGAC AGCGACTCAA TTGAAAAGGC TCTTCTAAAA 
TCGTGTAAAG ACGCCAAAGG GAAAGAAAAC CGATTTTGTT ATTATATAGG TGCAACGAGT 
GATGCAGCAA CAAAGATTAC CAATGAAGTT TCCAAACCAA TGAGTTACCA TGTGCCAGTG 
GAAAAGATCT GCGAAAAGCT TAAAAAGAAG GACAGTCAAA TCTGTGAACT CAAATACGAT 
AAACAAGTTG ACTTGAGCTC CGTCGACCTG AAGAAGCTGA AAGTGAAAGA TCTGAAGAAA 
ATTTTGGAGG AATGGGGCGA GTCCTGCAAA GGATGCGTGG AGAAATCTGA CTTCATCCGC 
AAAATCAATG AGCTGATGCC CAAGTATGCA CCCAGCGCAG CCAAGGCACG GACAGATCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070097.1
CDS	98..640
Translation	MLYLSGLSVAFALALVPSCSDALKDGECEVCVGFLQRLYQTIQENNVKFDSDSIEKALLKSCKDAKGKENRFCYYIGATSDAATKITNEVSKPMSYHVPVEKICEKLKKKDSQICELKYDKQVDLSSVDLKKLKVKDLKKILEEWGESCKGCVEKSDFIRKINELMPKYAPSAAKARTDL

Target ORF information:

RefSeq Version	NM_001076629.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mesencephalic astrocyte-derived neurotrophic factor (manf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076629.1

ORF Insert Sequence:

ATGTTATATT TAAGTGGACT TTCTGTGGCA TTTGCTCTCG CGCTGGTTCC GAGCTGCAGT 
GATGCTTTGA AAGATGGAGA GTGTGAAGTC TGTGTGGGTT TCCTCCAGCG GCTTTACCAG 
ACGATACAAG AAAACAACGT CAAGTTTGAC AGCGACTCAA TTGAAAAGGC TCTTCTAAAA 
TCGTGTAAAG ACGCCAAAGG GAAAGAAAAC CGATTTTGTT ATTATATAGG TGCAACGAGT 
GATGCAGCAA CAAAGATTAC CAATGAAGTT TCCAAACCAA TGAGTTACCA TGTGCCAGTG 
GAAAAGATCT GCGAAAAGCT TAAAAAGAAG GACAGTCAAA TCTGTGAACT CAAATACGAT 
AAACAAGTTG ACTTGAGCTC CGTCGACCTG AAGAAGCTGA AAGTGAAAGA TCTGAAGAAA 
ATTTTGGAGG AATGGGGCGA GTCCTGCAAA GGATGCGTGG AGAAATCTGA CTTCATCCGC 
AAAATCAATG AGCTGATGCC CAAGTATGCA CCCAGCGCAG CCAAGGCACG GACAGATCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enhanced uptake of BPA in the presence of nanoplastics can lead to neurotoxic effects in adult zebrafish.
The Science of the total environment6091312-1321(2017 Dec)
Chen Q,Yin D,Jia Y,Schiwy S,Legradi J,Yang S,Hollert H

Parental transfer of microcystin-LR induced transgenerational effects of developmental neurotoxicity in zebrafish offspring.
Environmental pollution (Barking, Essex : 1987)231(Pt 1)471-478(2017 Dec)
Wu Q,Yan W,Cheng H,Liu C,Hung TC,Guo X,Li G

A Mixture of Persistent Organic Pollutants and Perfluorooctanesulfonic Acid Induces Similar Behavioural Responses, but Different Gene Expression Profiles in Zebrafish Larvae.
International journal of molecular sciences18(2)(2017 Jan)
Khezri A,Fraser TW,Nourizadeh-Lillabadi R,Kamstra JH,Berg V,Zimmer KE,Ropstad E

A Novel Developmental Role for Dopaminergic Signaling to Specify Hypothalamic Neurotransmitter Identity.
The Journal of biological chemistry291(42)21880-21892(2016 Oct)
Chen YC,Semenova S,Rozov S,Sundvik M,Bonkowsky JL,Panula P