ifit8 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ifit8
Entrez Gene ID	641567
Full Name	interferon-induced protein with tetratricopeptide repeats 8
Synonyms	IFIT13,zgc:123282
General protein information	Preferred Names interferon-induced protein with tetratricopeptide repeats 8 Names uncharacterized protein LOC641567 Ifit13a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037565.1	NP_001032654.1	uncharacterized protein LOC641567

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Cellular visualization of macrophage pyroptosis and interleukin-1? release in a viral hemorrhagic infection in zebrafish larvae.
Varela M, Romero A, Dios S, van der Vaart M, Figueras A, Meijer AH, Novoa B
Journal of virology88(20)12026-40(2014 Oct)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

Interferon-induced genes of the expanded IFIT family show conserved antiviral activities in non-mammalian species.
Varela M, Diaz-Rosales P, Pereiro P, Forn-Cun? G, Costa MM, Dios S, Romero A, Figueras A, Novoa B
PloS one9(6)e100015(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ifit8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ifit8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35721	NM_001037565.1 Latest version!	Danio rerio interferon-induced protein with tetratricopeptide repeats 8 (ifit8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ifit8 ( NM_001037565.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032654.1 Danio rerio interferon-induced protein with tetratricopeptide repeats 8 (ifit8), mRNA.

Price & Availability↑

CloneID	ODa35721
Clone ID Related Accession (Same CDS sequence)	NM_001037565.1
Accession Version	NM_001037565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC641567
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC110122.1. ##Evidence-Data-START## Transcript exon combination :: BC110122.1, BC154134.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA4476759 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGAGG AAAATCTCAA ACTGTTGTCC TGCCACTTTA CATGGGGTCT GCTAAAAGAG 
GATGCTGATC TTAATTTCCT TGAAGTGAAA ATCCGTGAAA GACTGGGGGT AAAGAGCGGG 
TATGAAGGAA ACCTGAAACA GAGAGAATTA AATTTTCTGG CGTTCATTAA GCATCTTCAA 
GGATTCAACG ATGAGGCTCT CAAGAATCTG CTACTGGCAA AGGAAGAACA TCCAAATGAC 
GACAGGACTG TGATTGTGAC GTATGGAAAC CTGGCCTGGG TGCATAGTTT CCTGAGCAAT 
GCGACTGAAG CGGAGGCCTA CATAGAGAAA ATAAATGAAA TCCTCAGAGC TTTCCCCACT 
CCAGAGCTTC ATAGAGAAGT TCTGAGTGAA AAAGCCTGGT CACTTCTCAA GTTCTCTAAG 
AAGTCCTACA TCAGGGCCAA GGAGATTTTC CTTGAAGCTC TACAGAAAGA GCCGGATGAC 
AAGGAGTGGA ACACAGGCTT GGCGTTTTCT CTGTTCCGTC TGGAGGGACT GAAGATCGGT 
CAGTATCATC GAGTTCGTTT TGAAGAGTCT CCTGCTGTGC TTCAGTTGAA GAAAGCTTTA 
AAACTGGACC CGGATAATCC AATGATCCTG GTGTATCTGG GGCTCAAGTG CTACAAGAAC 
ATAAAGAACT CGGAAGCGTG GCAGTACATG ACGCAAGCAC TCAAAATGGC TCCCCATAAC 
CTCAGCGTTG TGTTGCACGT TGCAAAGTTC ATGAAGAAAG AGCAATGTTA TGACAGAGCC 
CTAGATGTGC TGCTGGAAAT GCTGAAGAAA GCACCAGATT CTTCTCGTTT GCATCATGAG 
GTTGCTAATA ATTACCGCTG GAAAGCCATG CAGATTGATG ACATACACAA TCCGGAGTTG 
CTGGGCCTCT GCGTTCACCA TTTAGAGAAG GGTGCCAGTT TAAACCCAGG TTATGTATAT 
CCACAGCTGG AGCTGGCGCT GAGGTATGCA GAGCTAAAGC AGGCAGGCAA AGCAGAGCAG 
AAGTTCACTG AGCTCTTCAG CAAGCCTGAC CTCAAGCCAG CTGATCTTCA AGCATGGCAC 
AGGCTGTATG GTGATTTCAA ATTGTACAGG CTGGGCTCAG AGAAAGCTGC AGTTGAGCAT 
TACAAGCAAG GCATGATGCT GGGACGAGTG TCCACCGAAT GGATCTCCTG CAGAAACAGA 
CTAAAGAAAA TCCTTCAGCG TGACAAACGG GACATCTACG AGATCCGAGT GTTCTTTGAT 
TCTATTAGAA CAACAAACAC AGATGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032654.1
CDS	143..1432
Translation	MSEENLKLLSCHFTWGLLKEDADLNFLEVKIRERLGVKSGYEGNLKQRELNFLAFIKHLQGFNDEALKNLLLAKEEHPNDDRTVIVTYGNLAWVHSFLSNATEAEAYIEKINEILRAFPTPELHREVLSEKAWSLLKFSKKSYIRAKEIFLEALQKEPDDKEWNTGLAFSLFRLEGLKIGQYHRVRFEESPAVLQLKKALKLDPDNPMILVYLGLKCYKNIKNSEAWQYMTQALKMAPHNLSVVLHVAKFMKKEQCYDRALDVLLEMLKKAPDSSRLHHEVANNYRWKAMQIDDIHNPELLGLCVHHLEKGASLNPGYVYPQLELALRYAELKQAGKAEQKFTELFSKPDLKPADLQAWHRLYGDFKLYRLGSEKAAVEHYKQGMMLGRVSTEWISCRNRLKKILQRDKRDIYEIRVFFDSIRTTNTDD

Target ORF information:

RefSeq Version	NM_001037565.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interferon-induced protein with tetratricopeptide repeats 8 (ifit8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037565.1

ORF Insert Sequence:

ATGTCAGAGG AAAATCTCAA ACTGTTGTCC TGCCACTTTA CATGGGGTCT GCTAAAAGAG 
GATGCTGATC TTAATTTCCT TGAAGTGAAA ATCCGTGAAA GACTGGGGGT AAAGAGCGGG 
TATGAAGGAA ACCTGAAACA GAGAGAATTA AATTTTCTGG CGTTCATTAA GCATCTTCAA 
GGATTCAACG ATGAGGCTCT CAAGAATCTG CTACTGGCAA AGGAAGAACA TCCAAATGAC 
GACAGGACTG TGATTGTGAC GTATGGAAAC CTGGCCTGGG TGCATAGTTT CCTGAGCAAT 
GCGACTGAAG CGGAGGCCTA CATAGAGAAA ATAAATGAAA TCCTCAGAGC TTTCCCCACT 
CCAGAGCTTC ATAGAGAAGT TCTGAGTGAA AAAGCCTGGT CACTTCTCAA GTTCTCTAAG 
AAGTCCTACA TCAGGGCCAA GGAGATTTTC CTTGAAGCTC TACAGAAAGA GCCGGATGAC 
AAGGAGTGGA ACACAGGCTT GGCGTTTTCT CTGTTCCGTC TGGAGGGACT GAAGATCGGT 
CAGTATCATC GAGTTCGTTT TGAAGAGTCT CCTGCTGTGC TTCAGTTGAA GAAAGCTTTA 
AAACTGGACC CGGATAATCC AATGATCCTG GTGTATCTGG GGCTCAAGTG CTACAAGAAC 
ATAAAGAACT CGGAAGCGTG GCAGTACATG ACGCAAGCAC TCAAAATGGC TCCCCATAAC 
CTCAGCGTTG TGTTGCACGT TGCAAAGTTC ATGAAGAAAG AGCAATGTTA TGACAGAGCC 
CTAGATGTGC TGCTGGAAAT GCTGAAGAAA GCACCAGATT CTTCTCGTTT GCATCATGAG 
GTTGCTAATA ATTACCGCTG GAAAGCCATG CAGATTGATG ACATACACAA TCCGGAGTTG 
CTGGGCCTCT GCGTTCACCA TTTAGAGAAG GGTGCCAGTT TAAACCCAGG TTATGTATAT 
CCACAGCTGG AGCTGGCGCT GAGGTATGCA GAGCTAAAGC AGGCAGGCAA AGCAGAGCAG 
AAGTTCACTG AGCTCTTCAG CAAGCCTGAC CTCAAGCCAG CTGATCTTCA AGCATGGCAC 
AGGCTGTATG GTGATTTCAA ATTGTACAGG CTGGGCTCAG AGAAAGCTGC AGTTGAGCAT 
TACAAGCAAG GCATGATGCT GGGACGAGTG TCCACCGAAT GGATCTCCTG CAGAAACAGA 
CTAAAGAAAA TCCTTCAGCG TGACAAACGG GACATCTACG AGATCCGAGT GTTCTTTGAT 
TCTATTAGAA CAACAAACAC AGATGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Cellular visualization of macrophage pyroptosis and interleukin-1? release in a viral hemorrhagic infection in zebrafish larvae.
Journal of virology88(20)12026-40(2014 Oct)
Varela M,Romero A,Dios S,van der Vaart M,Figueras A,Meijer AH,Novoa B

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

Interferon-induced genes of the expanded IFIT family show conserved antiviral activities in non-mammalian species.
PloS one9(6)e100015(2014)
Varela M,Diaz-Rosales P,Pereiro P,Forn-Cun? G,Costa MM,Dios S,Romero A,Figueras A,Novoa B