nedd4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nedd4a
Entrez Gene ID	619412
Full Name	neural precursor cell expressed, developmentally down-regulated 4a
Synonyms	si:ch211-79l17.5
General protein information	Preferred Names neural precursor cell expressed, developmentally down-regulated 4a Names E3 ubiquitin-protein ligase NEDD4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034186.1	NP_001029358.1	E3 ubiquitin-protein ligase NEDD4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	nedd4a	NP_001029358.1
Mus musculus (house mouse)	Nedd4	NP_035020.2
Schizosaccharomyces pombe (fission yeast)	pub3	NP_595793.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003133	XP_003436401.1
Pan troglodytes (chimpanzee)	NEDD4	XP_523083.3
Rattus norvegicus (Norway rat)	Nedd4	NP_037118.1

Gene Ontology
Bibliography

Related articles in PubMed

G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition.
Zhang P, He Q, Chen D, Liu W, Wang L, Zhang C, Ma D, Li W, Liu B, Liu F
Cell research25(10)1093-107(2015 Oct)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Itch is required for lateral line development in zebrafish.
Angers A, Drapeau P
PloS one9(11)e111799(2014)

Nedd4-1 binds and ubiquitylates activated FGFR1 to control its endocytosis and function.
Persaud A, Alberts P, Hayes M, Guettler S, Clarke I, Sicheri F, Dirks P, Ciruna B, Rotin D
The EMBO journal30(16)3259-73(2011 Jul)

Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs.
Hsu RJ, Yang HJ, Tsai HJ
Nucleic acids research37(10)e77(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nedd4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nedd4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98398	NM_001034186.1 Latest version!	Danio rerio NEDD4 E3 ubiquitin protein ligase a (nedd4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nedd4a ( NM_001034186.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001029358.1 Danio rerio NEDD4 E3 ubiquitin protein ligase a (nedd4a), mRNA.

Price & Availability↑

CloneID	ODa98398
Clone ID Related Accession (Same CDS sequence)	NM_001034186.1
Accession Version	NM_001034186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY995154.1. ##Evidence-Data-START## Transcript exon combination :: AY995154.1 [ECO:0000332] ##Evidence-Data-END##

ATGAACATGG CCGCGCAACT CCCGGAGGTT CGAGGACTCC AAACCGATGA GGATGAATCC 
AGAATCTTAA GGGTCAAAAT CATTGCTGGA ATTGGTTTGG CCAAGAAAGA TATTTTGGGT 
GCAAGTGATC CGTACACTCG AATCTCTCTC TATGACCCAG TCAATGGGGA AATCACCAGT 
CTACAAACCA AGACCATTAA AAAGACTTTG GATCCGAAGT GGAATGAAGA GTTCTTCTTT 
AGAGTGCATC CCAGGAGACA CCGTCTGCTG CTGGAGGTGT TTGATGAAAA TCGTTTGACA 
AGGGATGACT TCCTCGGACA AGTGGATGTT CCTCTGCACC AGATCCCTAC AGAACATCCA 
AACAATGAAA GGCCGTACAC TTTCAAAGAC TTCTTATTGC ACCCGAGAAG TCACAAGTCG 
AGGGTGAAAG GTCATCTGCG GCTAAAGATG ACGTACCTGC CTAAAAACAG GCGATGCAAG 
GACGACCCCG CTGAACAGGG CGAGAATCTG GATCCGGGTT GGGAGTTTTT GGAGCCTCAT 
GAATTATGCA GTCCTCATCA TCCGCATCAG CTGCCTGCGC TGCCCCCTGG CTGGGAGGAG 
AGGCAGGACA ACCTGGGACG AACTTACTAC GTCAACCACG AGAGCAGGAC CACACAGTGG 
CACAGACCCA CCATCCAGGA CAGTCAAAGA GACAATGAGC AGAGACAGGA AACACACAGC 
GAGTCCCAGC GGGCCTTTCT CACACGCAGA CAAATATCAG AACATGAGGA GAACCACGAG 
CGCAGAGAGT CACCTGAGAG CTGGGAGATC ATAACAGCAG ATGAGTCGAC GCTGTACAGC 
AGCCTCAGTG AGCGCTCGCC GCCGCCGCCA CACTCCCCGA TGGAGATCAG CACTTTCTGT 
GAGGAGTTCA ACAGACTGCA GGCCACAAAC GACAGACGCA CGTCCCTCAC GCCGCAGGTC 
ACGGCAGCCG CAGAGGAAGT GGACAGACCC TCACGATGGA GGAGCATCCT GTTCACCCAC 
TCGTTATTGC CCACCTCCGC CGGCCTGCCT CCGGGATGGG AGGAAAAGCA GGACAGCAAA 
GGAAGAATTT ATTTTGTCAA TCATAACAGC AGAACCACAA CCTGGACACG GCCACTCATT 
CAGCTCATAC AGCCGCCCTC AGAGTCTCCA GCATCCCCGA TGGCCAGCGG ATCAACGTCT 
CTTTCCCAGA ATGCCGCAGG CTACGCTCTG CCCCCAGACG TGTCCCCACA GCACACACAC 
ACTCACACAC ACACTCACAC GCCGGAGCAC CCAGGACTGA TGCCGACTGG CTGGGAAGTG 
CGCAGCGCTC CAAGCGGACG GCCCTTCTTC ATCGACCACA ACACCAAGAC CACCACATGG 
GACGACCCGC GGCTGAAGGT TCCAGTGCAG ATGAGGAGGA GAGTCTCCCT CGACCCCACT 
GACCTCGGCC CGCTGCCGCC TGGTTGGGAG AGCGAGTGCA TAGTGATGGC AGAATCTTCT 
ACATTGATCA CAACACAAAA ACCACACAAT GGGAAGACCC ACGACTACAG AATTCAGCCA 
TCACTTGGTC CAGCCGTGCC TTACTCCAGA GACTATAAAC AGAAGTACGA GTATTTCCGC 
AAGAAACTCA AGAAACCGGC AGAGATCCCG AATCGCTTCG AGCTGAGTGT GCGGCGTAAC 
GCAGTGCTGG AGGACTCATA CAGACGCATC CTGTCCGTCA AGCGCTCGGA GCTGCTGAAG 
GCCCGCCTCT GGGTGGAGTT TGAGGGAGAA AAGGGGCTGG ACTATGGGGG CGTGGCCAGA 
GAGTGGTTCT TCTTGATCTC CAAGGAAATG TTCAATCCGT ATTATGGGCT GTTTGAGTAT 
TCAGCCACGG ACAACTACAC CCTGCAGATC AACCCAAACT CAGGTCTGTG TAACGAGGAC 
CACCTCTCCT ACTTTAAGTT CATCGGCCGG GTGGCTGGTA TGGCGGTTTA TCACGGGAAG 
CTGCTGGATG CATTCTTCAT CCGACCATTC TACAAGATGA TGCTGCAGAA GCCAATCACG 
TTACAGGATA TGGAGTCAGT GGACAGCGAG TATTTTAATT CCCTCAGGTG GATCCTAGAA 
AACGACCCCA CAGACCTGGA CCTCCGGTTC ACCATCGACG AGGAGCTTTT TGGACAGACG 
CACCAACATG AGCTGAAACC AGGAGGTGCT GATATCGTGG TCAATGACAC CAACAAGAAG 
GAGTACATTC ATCTGGTGAT GCAGTGGAGG TTTGTGGATC GCATACAAAG ACAGATGACG 
GCCTTTAAGG AGGGTTTCTA TGAGCTGATT CCTCAGGACC TGATCAAGAT CTTTGATGAA 
AATGAGCTGG AGCTGCTGAT GTGTGGTTTA GGAGACGTGG ACGTCAACGA CTGGAGAGAA 
AACACCAAAT ATAAAAATGG CTACAACCCA AATCATCCCG CCATCATCTG GTTCTGGAAG 
ACGGTGTTGC TGATGGATGC TGAAAAGCGA ATCCGTTTGC TGCAGTTTGT GACGGGAACA 
TCTCGCGTAC CAATGAATGG CTTCGCAGAA CTCTACGGAT CCAACGGGCC TCAGTTGTTC 
ACCATCGAGC AGTGGGGCAC GAGGGAAAAA CTGCCCCGAG CACACACCTG CTTCAACAGA 
CTGGATCTTC CTCCGYACGA GTCGTTTGAG GAGCTGCGAG AGAAGCTGCA CATGGCCATC 
GAGAACGCAC AAGGCTTTGA TGGAGTCGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029358.1
CDS	1..2733
Translation	MNMAAQLPEVRGLQTDEDESRILRVKIIAGIGLAKKDILGASDPYTRISLYDPVNGEITSLQTKTIKKTLDPKWNEEFFFRVHPRRHRLLLEVFDENRLTRDDFLGQVDVPLHQIPTEHPNNERPYTFKDFLLHPRSHKSRVKGHLRLKMTYLPKNRRCKDDPAEQGENLDPGWEFLEPHELCSPHHPHQLPALPPGWEERQDNLGRTYYVNHESRTTQWHRPTIQDSQRDNEQRQETHSESQRAFLTRRQISEHEENHERRESPESWEIITADESTLYSSLSERSPPPPHSPMEISTFCEEFNRLQATNDRRTSLTPQVTAAAEEVDRPSRWRSILFTHSLLPTSAGLPPGWEEKQDSKGRIYFVNHNSRTTTWTRPLIQLIQPPSESPASPMASGSTSLSQNAAGYALPPDVSPQHTHTHTHTHTPEHPGLMPTGWEVRSAPSGRPFFIDHNTKTTTWDDPRLKVPVQMRRRVSLDPTDLGPLPPGWESECIVMAESSTLITTQKPHNGKTHDYRIQPSLGPAVPYSRDYKQKYEYFRKKLKKPAEIPNRFELSVRRNAVLEDSYRRILSVKRSELLKARLWVEFEGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDAFFIRPFYKMMLQKPITLQDMESVDSEYFNSLRWILENDPTDLDLRFTIDEELFGQTHQHELKPGGADIVVNDTNKKEYIHLVMQWRFVDRIQRQMTAFKEGFYELIPQDLIKIFDENELELLMCGLGDVDVNDWRENTKYKNGYNPNHPAIIWFWKTVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTREKLPRAHTCFNRLDLPPnullESFEELREKLHMAIENAQGFDGVD

Target ORF information:

RefSeq Version	NM_001034186.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NEDD4 E3 ubiquitin protein ligase a (nedd4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034186.1

ORF Insert Sequence:

ATGAACATGG CCGCGCAACT CCCGGAGGTT CGAGGACTCC AAACCGATGA GGATGAATCC 
AGAATCTTAA GGGTCAAAAT CATTGCTGGA ATTGGTTTGG CCAAGAAAGA TATTTTGGGT 
GCAAGTGATC CGTACACTCG AATCTCTCTC TATGACCCAG TCAATGGGGA AATCACCAGT 
CTACAAACCA AGACCATTAA AAAGACTTTG GATCCGAAGT GGAATGAAGA GTTCTTCTTT 
AGAGTGCATC CCAGGAGACA CCGTCTGCTG CTGGAGGTGT TTGATGAAAA TCGTTTGACA 
AGGGATGACT TCCTCGGACA AGTGGATGTT CCTCTGCACC AGATCCCTAC AGAACATCCA 
AACAATGAAA GGCCGTACAC TTTCAAAGAC TTCTTATTGC ACCCGAGAAG TCACAAGTCG 
AGGGTGAAAG GTCATCTGCG GCTAAAGATG ACGTACCTGC CTAAAAACAG GCGATGCAAG 
GACGACCCCG CTGAACAGGG CGAGAATCTG GATCCGGGTT GGGAGTTTTT GGAGCCTCAT 
GAATTATGCA GTCCTCATCA TCCGCATCAG CTGCCTGCGC TGCCCCCTGG CTGGGAGGAG 
AGGCAGGACA ACCTGGGACG AACTTACTAC GTCAACCACG AGAGCAGGAC CACACAGTGG 
CACAGACCCA CCATCCAGGA CAGTCAAAGA GACAATGAGC AGAGACAGGA AACACACAGC 
GAGTCCCAGC GGGCCTTTCT CACACGCAGA CAAATATCAG AACATGAGGA GAACCACGAG 
CGCAGAGAGT CACCTGAGAG CTGGGAGATC ATAACAGCAG ATGAGTCGAC GCTGTACAGC 
AGCCTCAGTG AGCGCTCGCC GCCGCCGCCA CACTCCCCGA TGGAGATCAG CACTTTCTGT 
GAGGAGTTCA ACAGACTGCA GGCCACAAAC GACAGACGCA CGTCCCTCAC GCCGCAGGTC 
ACGGCAGCCG CAGAGGAAGT GGACAGACCC TCACGATGGA GGAGCATCCT GTTCACCCAC 
TCGTTATTGC CCACCTCCGC CGGCCTGCCT CCGGGATGGG AGGAAAAGCA GGACAGCAAA 
GGAAGAATTT ATTTTGTCAA TCATAACAGC AGAACCACAA CCTGGACACG GCCACTCATT 
CAGCTCATAC AGCCGCCCTC AGAGTCTCCA GCATCCCCGA TGGCCAGCGG ATCAACGTCT 
CTTTCCCAGA ATGCCGCAGG CTACGCTCTG CCCCCAGACG TGTCCCCACA GCACACACAC 
ACTCACACAC ACACTCACAC GCCGGAGCAC CCAGGACTGA TGCCGACTGG CTGGGAAGTG 
CGCAGCGCTC CAAGCGGACG GCCCTTCTTC ATCGACCACA ACACCAAGAC CACCACATGG 
GACGACCCGC GGCTGAAGGT TCCAGTGCAG ATGAGGAGGA GAGTCTCCCT CGACCCCACT 
GACCTCGGCC CGCTGCCGCC TGGTTGGGAG AGCGAGTGCA TAGTGATGGC AGAATCTTCT 
ACATTGATCA CAACACAAAA ACCACACAAT GGGAAGACCC ACGACTACAG AATTCAGCCA 
TCACTTGGTC CAGCCGTGCC TTACTCCAGA GACTATAAAC AGAAGTACGA GTATTTCCGC 
AAGAAACTCA AGAAACCGGC AGAGATCCCG AATCGCTTCG AGCTGAGTGT GCGGCGTAAC 
GCAGTGCTGG AGGACTCATA CAGACGCATC CTGTCCGTCA AGCGCTCGGA GCTGCTGAAG 
GCCCGCCTCT GGGTGGAGTT TGAGGGAGAA AAGGGGCTGG ACTATGGGGG CGTGGCCAGA 
GAGTGGTTCT TCTTGATCTC CAAGGAAATG TTCAATCCGT ATTATGGGCT GTTTGAGTAT 
TCAGCCACGG ACAACTACAC CCTGCAGATC AACCCAAACT CAGGTCTGTG TAACGAGGAC 
CACCTCTCCT ACTTTAAGTT CATCGGCCGG GTGGCTGGTA TGGCGGTTTA TCACGGGAAG 
CTGCTGGATG CATTCTTCAT CCGACCATTC TACAAGATGA TGCTGCAGAA GCCAATCACG 
TTACAGGATA TGGAGTCAGT GGACAGCGAG TATTTTAATT CCCTCAGGTG GATCCTAGAA 
AACGACCCCA CAGACCTGGA CCTCCGGTTC ACCATCGACG AGGAGCTTTT TGGACAGACG 
CACCAACATG AGCTGAAACC AGGAGGTGCT GATATCGTGG TCAATGACAC CAACAAGAAG 
GAGTACATTC ATCTGGTGAT GCAGTGGAGG TTTGTGGATC GCATACAAAG ACAGATGACG 
GCCTTTAAGG AGGGTTTCTA TGAGCTGATT CCTCAGGACC TGATCAAGAT CTTTGATGAA 
AATGAGCTGG AGCTGCTGAT GTGTGGTTTA GGAGACGTGG ACGTCAACGA CTGGAGAGAA 
AACACCAAAT ATAAAAATGG CTACAACCCA AATCATCCCG CCATCATCTG GTTCTGGAAG 
ACGGTGTTGC TGATGGATGC TGAAAAGCGA ATCCGTTTGC TGCAGTTTGT GACGGGAACA 
TCTCGCGTAC CAATGAATGG CTTCGCAGAA CTCTACGGAT CCAACGGGCC TCAGTTGTTC 
ACCATCGAGC AGTGGGGCAC GAGGGAAAAA CTGCCCCGAG CACACACCTG CTTCAACAGA 
CTGGATCTTC CTCCGYACGA GTCGTTTGAG GAGCTGCGAG AGAAGCTGCA CATGGCCATC 
GAGAACGCAC AAGGCTTTGA TGGAGTCGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition.
Cell research25(10)1093-107(2015 Oct)
Zhang P,He Q,Chen D,Liu W,Wang L,Zhang C,Ma D,Li W,Liu B,Liu F

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Itch is required for lateral line development in zebrafish.
PloS one9(11)e111799(2014)
Angers A,Drapeau P

Nedd4-1 binds and ubiquitylates activated FGFR1 to control its endocytosis and function.
The EMBO journal30(16)3259-73(2011 Jul)
Persaud A,Alberts P,Hayes M,Guettler S,Clarke I,Sicheri F,Dirks P,Ciruna B,Rotin D

Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs.
Nucleic acids research37(10)e77(2009 Jun)
Hsu RJ,Yang HJ,Tsai HJ