usp12a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp12a
Entrez Gene ID	565736
Full Name	ubiquitin specific peptidase 12a
Synonyms	sb:eu882,usp12,wu:fi09d12
General protein information	Preferred Names ubiquitin specific peptidase 12a Names ubiquitin carboxyl-terminal hydrolase 12A deubiquitinating enzyme 12A ubiquitin specific peptidase 12 ubiquitin thioesterase 12A ubiquitin thiolesterase 12A ubiquitin-specific-processing protease 12A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083556.1	NP_001077025.1	ubiquitin carboxyl-terminal hydrolase 12A

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP12	NP_872294.2
Bos taurus (cattle)	USP12	NP_001091528.1
Rattus norvegicus (Norway rat)	Usp12	NP_001160048.1
Schizosaccharomyces pombe (fission yeast)	ubp9	NP_596207.1
Pan troglodytes (chimpanzee)	USP12	XP_001135418.2
Canis lupus familiaris (dog)	USP12	XP_543159.2
Mus musculus (house mouse)	Usp12	NP_035799.1
Gallus gallus (chicken)	USP12	XP_004938833.1
Oryza sativa (rice)	Os03g0190800	NP_001049228.1
Danio rerio (zebrafish)	usp12a	NP_001077025.1
Xenopus tropicalis (tropical clawed frog)	LOC100494517	XP_002941584.2
Neurospora crassa	NCU08378	XP_965281.2
	LOC721611	XP_001117796.2
Magnaporthe oryzae (rice blast fungus)	MGG_09907	XP_003710004.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following usp12a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp12a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38218	NM_001083556.1 Latest version!	Danio rerio ubiquitin specific peptidase 12a (usp12a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp12a ( NM_001083556.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077025.1 Danio rerio ubiquitin specific peptidase 12a (usp12a), mRNA.

Price & Availability↑

CloneID	ODa38218
Clone ID Related Accession (Same CDS sequence)	NM_001083556.1
Accession Version	NM_001083556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 12A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC115286.1. On Mar 28, 2007 this sequence version replaced XM_689009.2. ##Evidence-Data-START## Transcript exon combination :: BC115286.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAAATCC TAATGACAGT TTCCAAATTT GCCTCTTTTT GTACCATGGG CGCCAATGCC 
TCCGCTTTGG AGAAAGAGAT CGGCTCGGAG CAGTTCCCAG TCAACGAGCA TTACTTCGGA 
TTGGTCAACT TCGGAAACAC CTGCTACTGT AACTCTGTGC TCCAGGCTCT GTACTTCTGC 
CGGCCGTTTC GGGAGAAGAT CCTTGCGTAC AGGAGTCAAC CGCGCCGCAA GGAGAACCTG 
CTCACCTGCC TGGCTGACCT GTTCCACAGT ATAGCCAACC AGAAGAGGAA AGTTGGCGTT 
ATACCGCCGA AGAAGTTCAT TACGCGACTA CGGAAAGAGA ATGAACTGTT TGATAACTAC 
ATGCAGCAAG ATGCACATGA GTTTCTCAAC TACCTGCTGA ACACTATAGC AGATCTGCTC 
CAAGAAGAGC GCAAACAAGA CAAGCAGAAT GGCAAGCTTG CCAACGGCAC ACTCGATTCC 
CAGAACAATA ACAGCACCCC ACCCTCCACC ACCTGGGTTC ATGAGATCTT CCAGGGCACG 
TTGACCAATG AGACGCGATG CCTCACCTGC GAAACGATAA GCAGCAAAGA TGAGGATTTC 
TTGGATTTGT CAGTGGACGT GGAGCAGAAC ACTTCAATCA CACACTGTCT CAGAGGTTTC 
AGTAATACAG AGACGTTGTG CAGTGAATAT AAATATTACT GTGAGGAATG CAGAAGTAAA 
CAGGAGGCAC ACAAACGAAT GCGGGTTAAG AAACTTCCCA TGATCCTCGC CTTGCACTTG 
AAGAGATTTA AATACATGGA GCAGCTGCAG CGCTACACTA AGCTAAGCTA CCGTGTGGTC 
TTCCCTCTGG AGCTCCGCCT CTTTAACACC TCTGGTGACG CCACCAATCC CGAGAGGCTT 
TACGACCTGG TCGCTGTGGT GGTGCATTGT GGCAGTGGTC CAAACCGAGG ACACTACATC 
GCAATTGTAA AAAGTCATGA TTTCTGGCTA CTGTTTGATG ACGACATTGT AGAGAAAATC 
GATGCCCAGG CGATTGAAGA GTTCTACGGA TTAACCTCGG AAATTTCCAA GAACTCTGAG 
TCTGGCTACA TCCTGTTTTA CCAGTCCAGA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077025.1
CDS	19..1134
Translation	MEILMTVSKFASFCTMGANASALEKEIGSEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKILAYRSQPRRKENLLTCLADLFHSIANQKRKVGVIPPKKFITRLRKENELFDNYMQQDAHEFLNYLLNTIADLLQEERKQDKQNGKLANGTLDSQNNNSTPPSTTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMRVKKLPMILALHLKRFKYMEQLQRYTKLSYRVVFPLELRLFNTSGDATNPERLYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGLTSEISKNSESGYILFYQSRD

Target ORF information:

RefSeq Version	NM_001083556.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 12a (usp12a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083556.1

ORF Insert Sequence:

ATGGAAATCC TAATGACAGT TTCCAAATTT GCCTCTTTTT GTACCATGGG CGCCAATGCC 
TCCGCTTTGG AGAAAGAGAT CGGCTCGGAG CAGTTCCCAG TCAACGAGCA TTACTTCGGA 
TTGGTCAACT TCGGAAACAC CTGCTACTGT AACTCTGTGC TCCAGGCTCT GTACTTCTGC 
CGGCCGTTTC GGGAGAAGAT CCTTGCGTAC AGGAGTCAAC CGCGCCGCAA GGAGAACCTG 
CTCACCTGCC TGGCTGACCT GTTCCACAGT ATAGCCAACC AGAAGAGGAA AGTTGGCGTT 
ATACCGCCGA AGAAGTTCAT TACGCGACTA CGGAAAGAGA ATGAACTGTT TGATAACTAC 
ATGCAGCAAG ATGCACATGA GTTTCTCAAC TACCTGCTGA ACACTATAGC AGATCTGCTC 
CAAGAAGAGC GCAAACAAGA CAAGCAGAAT GGCAAGCTTG CCAACGGCAC ACTCGATTCC 
CAGAACAATA ACAGCACCCC ACCCTCCACC ACCTGGGTTC ATGAGATCTT CCAGGGCACG 
TTGACCAATG AGACGCGATG CCTCACCTGC GAAACGATAA GCAGCAAAGA TGAGGATTTC 
TTGGATTTGT CAGTGGACGT GGAGCAGAAC ACTTCAATCA CACACTGTCT CAGAGGTTTC 
AGTAATACAG AGACGTTGTG CAGTGAATAT AAATATTACT GTGAGGAATG CAGAAGTAAA 
CAGGAGGCAC ACAAACGAAT GCGGGTTAAG AAACTTCCCA TGATCCTCGC CTTGCACTTG 
AAGAGATTTA AATACATGGA GCAGCTGCAG CGCTACACTA AGCTAAGCTA CCGTGTGGTC 
TTCCCTCTGG AGCTCCGCCT CTTTAACACC TCTGGTGACG CCACCAATCC CGAGAGGCTT 
TACGACCTGG TCGCTGTGGT GGTGCATTGT GGCAGTGGTC CAAACCGAGG ACACTACATC 
GCAATTGTAA AAAGTCATGA TTTCTGGCTA CTGTTTGATG ACGACATTGT AGAGAAAATC 
GATGCCCAGG CGATTGAAGA GTTCTACGGA TTAACCTCGG AAATTTCCAA GAACTCTGAG 
TCTGGCTACA TCCTGTTTTA CCAGTCCAGA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ