NLK2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLK2
Entrez Gene ID	563460
Full Name	nemo-like kinase, type 2
Synonyms	nlka
General protein information	Preferred Names nemo-like kinase, type 2 Names serine/threonine-protein kinase NLK nemo like kinase a nemo-like kinase 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113485.2	NP_001106957.2	serine/threonine-protein kinase NLK
XM_017350980.2	XP_017206469.1	serine/threonine-protein kinase NLK isoform X1
XM_017350980.1	XP_017206469.1	serine/threonine-protein kinase NLK isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nlk	NP_032728.3
Homo sapiens (human)	NLK	NP_057315.3
Gallus gallus (chicken)	NLK	XP_415915.3
Drosophila melanogaster (fruit fly)	nmo	NP_729318.1
Caenorhabditis elegans (roundworm)	lit-1	NP_001022806.1
Rattus norvegicus (Norway rat)	Nlk	NP_001178853.1
Danio rerio (zebrafish)	nlk2	NP_001106957.1
Pan troglodytes (chimpanzee)	NLK	XP_001147662.2
Canis lupus familiaris (dog)	NLK	XP_868108.2
Bos taurus (cattle)	NLK	NP_001180182.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005898	XP_315928.3
Xenopus tropicalis (tropical clawed frog)	nlk	NP_001116885.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

NLK positively regulates Wnt/?-catenin signalling by phosphorylating LEF1 in neural progenitor cells.
Ota S, Ishitani S, Shimizu N, Matsumoto K, Itoh M, Ishitani T
The EMBO journal31(8)1904-15(2012 Apr)

Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish.
Stein C, Caccamo M, Laird G, Leptin M
Genome biology8(11)R251(2007)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nlk2 is essential for the phosphorylation and activation of Lef1 transcriptional activity in neural progenitor cells.
Title: NLK positively regulates Wnt/?-catenin signalling by phosphorylating LEF1 in neural progenitor cells.

The following NLK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa98337	NM_001113485.2 Latest version!	Danio rerio nemo-like kinase, type 2 (nlk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa55104	XM_017350980.2 Latest version!	Danio rerio nemo-like kinase, type 2 (nlk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
ODa55104	XM_017350980.1	Danio rerio nemo-like kinase, type 2 (nlk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLK2 ( NM_001113485.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001106957.2 Danio rerio nemo-like kinase, type 2 (nlk2), mRNA.

Price & Availability↑

CloneID	ODa98337
Clone ID Related Accession (Same CDS sequence)	NM_001113485.2
Accession Version	NM_001113485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase NLK
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB372222.1. On Dec 23, 2015 this sequence version replaced NM_001113485.1. ##Evidence-Data-START## Transcript exon combination :: AB372222.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTCTGT GCGGAACCAC GGCTTCCAAT GCCGCGAAAA TGATGGCTGC GTACAACGGC 
GGCACCGCGG CAGTGGCAGC GCATCACCCG CACCATCACC ACCAGCTGCC ACATCTCCCG 
CCACCCGCCC ACCTCCACCA CCATCACCAC CACCAACACT CGCATCACTT ACAACAACAG 
CTGGATATAG AGCCGGACAG ACCGATCGGA TACGGAGCTT TCGGCGTGGT TTGGTCGGTG 
ACAGACCCCA GAGACGGCAA ACGCGTGGCA CTCAAAAAGA TGCCCAATGT CTTCCAAAAC 
CTGGTCTCGT GCAAGAGGGT TTTCCGTGAG CTGAAGATGC TTTGTTTCTT CAAGCACGAC 
AACGTGCTGT CAGCTCTGGA TATTCTGCAG CCTCCACACA TCGACTACTT TGAGGAAATT 
TATGTGGTGA CGGAGCTGAT GCAGAGCGAC CTCCATAAGA TCATTGTATC TCCACAACCT 
CTGAGCTCGG ACCACGTCAA AGTCTTTCTC TACCAGATCC TTCGTGGATT GAAGTATCTA 
CACTCAGCCG GCATTTTGCA CAGAGACATT AAACCTGGGA ACCTGCTGGT CAACAGCAAT 
TGTGTGCTAA AGATCTGTGA CTTTGGCCTG GCGCGGGTGG AGGAGCTGGA CGAGTCACGT 
CACATGACCC AGGAGGTGGT GACGCAGTAC TACAGAGCGC CGGAGATCCT CATGGGGAGC 
AGACATTACT CCAACGCCAT CGACATCTGG TCTGTGGGCT GCATTTTTGC TGAGCTGCTC 
GGCCGACGCA TCCTTTTCCA GGCCCAGAGT CCCATCCAAC AGCTGGACCT GATCACAGAT 
CTGCTAGGGA CGCCATCTCT GGAGGCCATG AGGACGGCCT GTGAGGGGGC CAGAGCTCAC 
ATTCTCAGAG GACCACACAA ACAGCCCTCA CTTCCAGTCC TATATACCCT GTCCAGTCAA 
GCAACCCATG AGGCCGTTCA CCTCCTCTGC AGGATGTTGG TCTTCGACCC ATCGAAGCGA 
ATCTCTGCGA AGGATGCCCT GGCACACCCG TATCTGGATG AGGGGCGTCT GCGATACCAC 
ACCTGCATGT GCAAATGCTG CTACACCACG TCCTCCGGCC GCGTTTACAC CAGTGACTTC 
GAACCCGTCA CAAACCCCAA GTTTGACGAT GGATTTGAGA AGAACCTGAC GTCAGTTCGG 
CAGGTCAAAG AGATCATTCA TCAGTTCATC CTGGAGCAGC AGAAAGGGAA TCGAGTACCG 
CTCTGTATCA ACCCTCAATC CGCTGCCTTC AAAAGCTTTA TCAGCTCAAC AGTAGCTCAG 
CCCTCTGAGA TGCCTCCATC TCCTTTAGTT TGGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106957.2
CDS	1081..2439
Translation	MALCGTTASNAAKMMAAYNGGTAAVAAHHPHHHHQLPHLPPPAHLHHHHHHQHSHHLQQQLDIEPDRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKICDFGLARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAELLGRRILFQAQSPIQQLDLITDLLGTPSLEAMRTACEGARAHILRGPHKQPSLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRYHTCMCKCCYTTSSGRVYTSDFEPVTNPKFDDGFEKNLTSVRQVKEIIHQFILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE

Target ORF information:

RefSeq Version	NM_001113485.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nemo-like kinase, type 2 (nlk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113485.2

ORF Insert Sequence:

ATGGCTCTGT GCGGAACCAC GGCTTCCAAT GCCGCGAAAA TGATGGCTGC GTACAACGGC 
GGCACCGCGG CAGTGGCAGC GCATCACCCG CACCATCACC ACCAGCTGCC ACATCTCCCG 
CCACCCGCCC ACCTCCACCA CCATCACCAC CACCAACACT CGCATCACTT ACAACAACAG 
CTGGATATAG AGCCGGACAG ACCGATCGGA TACGGAGCTT TCGGCGTGGT TTGGTCGGTG 
ACAGACCCCA GAGACGGCAA ACGCGTGGCA CTCAAAAAGA TGCCCAATGT CTTCCAAAAC 
CTGGTCTCGT GCAAGAGGGT TTTCCGTGAG CTGAAGATGC TTTGTTTCTT CAAGCACGAC 
AACGTGCTGT CAGCTCTGGA TATTCTGCAG CCTCCACACA TCGACTACTT TGAGGAAATT 
TATGTGGTGA CGGAGCTGAT GCAGAGCGAC CTCCATAAGA TCATTGTATC TCCACAACCT 
CTGAGCTCGG ACCACGTCAA AGTCTTTCTC TACCAGATCC TTCGTGGATT GAAGTATCTA 
CACTCAGCCG GCATTTTGCA CAGAGACATT AAACCTGGGA ACCTGCTGGT CAACAGCAAT 
TGTGTGCTAA AGATCTGTGA CTTTGGCCTG GCGCGGGTGG AGGAGCTGGA CGAGTCACGT 
CACATGACCC AGGAGGTGGT GACGCAGTAC TACAGAGCGC CGGAGATCCT CATGGGGAGC 
AGACATTACT CCAACGCCAT CGACATCTGG TCTGTGGGCT GCATTTTTGC TGAGCTGCTC 
GGCCGACGCA TCCTTTTCCA GGCCCAGAGT CCCATCCAAC AGCTGGACCT GATCACAGAT 
CTGCTAGGGA CGCCATCTCT GGAGGCCATG AGGACGGCCT GTGAGGGGGC CAGAGCTCAC 
ATTCTCAGAG GACCACACAA ACAGCCCTCA CTTCCAGTCC TATATACCCT GTCCAGTCAA 
GCAACCCATG AGGCCGTTCA CCTCCTCTGC AGGATGTTGG TCTTCGACCC ATCGAAGCGA 
ATCTCTGCGA AGGATGCCCT GGCACACCCG TATCTGGATG AGGGGCGTCT GCGATACCAC 
ACCTGCATGT GCAAATGCTG CTACACCACG TCCTCCGGCC GCGTTTACAC CAGTGACTTC 
GAACCCGTCA CAAACCCCAA GTTTGACGAT GGATTTGAGA AGAACCTGAC GTCAGTTCGG 
CAGGTCAAAG AGATCATTCA TCAGTTCATC CTGGAGCAGC AGAAAGGGAA TCGAGTACCG 
CTCTGTATCA ACCCTCAATC CGCTGCCTTC AAAAGCTTTA TCAGCTCAAC AGTAGCTCAG 
CCCTCTGAGA TGCCTCCATC TCCTTTAGTT TGGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLK2 ( XM_017350980.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206469.1 Danio rerio nemo-like kinase, type 2 (nlk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55104
Clone ID Related Accession (Same CDS sequence)	XM_017350980.2 , XM_017350980.1
Accession Version	XM_017350980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase NLK isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017350980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT GCGGAACCAC GGCTTCCAAT GCCGCGAAAA TGATGGCTGC GTACAACGGC 
GGCACCGCGG CAGTGGCAGC GCATCACCCG CACCATCACC ACCAGCTGCC ACATCTCCCG 
CCACCCGCCC ACCTCCACCA CCATCACCAC GCTGGCCAGC ATCACCTCCA GCACCCCGGC 
TCGGCCGCCG CCGTGCACCC CGTACAGCAG CACGCTTCTA CGGCCGCGGC CGCTGCTGCT 
GCGGCGGCGG CGGCGGTGAT GCTGAACCCG AGCCAGCAGC AGCCGTACTT CCCCTCGCCC 
GCTCCTGGAC AAGCTCCGGG GCCGGCGGCC GCTGCAGCCC CAGCACAGGT GCAAGCCGCC 
GCCGCGGCCG CTGTAAAGGT GCACCATCAC CACCACCAAC ACTCGCATCA CTTACAACAA 
CAGCTGGATA TAGAGCCGGA CAGACCGATC GGATACGGAG CTTTCGGCGT GGTTTGGTCG 
GTGACAGACC CCAGAGACGG CAAACGCGTG GCACTCAAAA AGATGCCCAA TGTCTTCCAA 
AACCTGGTCT CGTGCAAGAG GGTTTTCCGT GAGCTGAAGA TGCTTTGTTT CTTCAAGCAC 
GACAACGTGC TGTCAGCTCT GGATATTCTG CAGCCTCCAC ACATCGACTA CTTTGAGGAA 
ATTTATGTGG TGACGGAGCT GATGCAGAGC GACCTCCATA AGATCATTGT ATCTCCACAA 
CCTCTGAGCT CGGACCACGT CAAAGTCTTT CTCTACCAGA TCCTTCGTGG ATTGAAGTAT 
CTACACTCAG CCGGCATTTT GCACAGAGAC ATTAAACCTG GGAACCTGCT GGTCAACAGC 
AATTGTGTGC TAAAGATCTG TGACTTTGGC CTGGCGCGGG TGGAGGAGCT GGACGAGTCA 
CGTCACATGA CCCAGGAGGT GGTGACGCAG TACTACAGAG CGCCGGAGAT CCTCATGGGG 
AGCAGACATT ACTCCAACGC CATCGACATC TGGTCTGTGG GCTGCATTTT TGCTGAGCTG 
CTCGGCCGAC GCATCCTTTT CCAGGCCCAG AGTCCCATCC AACAGCTGGA CCTGATCACA 
GATCTGCTAG GGACGCCATC TCTGGAGGCC ATGAGGACGG CCTGTGAGGG GGCCAGAGCT 
CACATTCTCA GAGGACCACA CAAACAGCCC TCACTTCCAG TCCTATATAC CCTGTCCAGT 
CAAGCAACCC ATGAGGCCGT TCACCTCCTC TGCAGGATGT TGGTCTTCGA CCCATCGAAG 
CGAATCTCTG CGAAGGATGC CCTGGCACAC CCGTATCTGG ATGAGGGGCG TCTGCGATAC 
CACACCTGCA TGTGCAAATG CTGCTACACC ACGTCCTCCG GCCGCGTTTA CACCAGTGAC 
TTCGAACCCG TCACAAACCC CAAGTTTGAC GATGGATTTG AGAAGAACCT GACGTCAGTT 
CGGCAGGTCA AAGAGATCAT TCATCAGTTC ATCCTGGAGC AGCAGAAAGG GAATCGAGTA 
CCGCTCTGTA TCAACCCTCA ATCCGCTGCC TTCAAAAGCT TTATCAGCTC AACAGTAGCT 
CAGCCCTCTG AGATGCCTCC ATCTCCTTTA GTTTGGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206469.1
CDS	1171..2772
Translation	MALCGTTASNAAKMMAAYNGGTAAVAAHHPHHHHQLPHLPPPAHLHHHHHAGQHHLQHPGSAAAVHPVQQHASTAAAAAAAAAAAVMLNPSQQQPYFPSPAPGQAPGPAAAAAPAQVQAAAAAAVKVHHHHHQHSHHLQQQLDIEPDRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKICDFGLARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAELLGRRILFQAQSPIQQLDLITDLLGTPSLEAMRTACEGARAHILRGPHKQPSLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRYHTCMCKCCYTTSSGRVYTSDFEPVTNPKFDDGFEKNLTSVRQVKEIIHQFILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE

Target ORF information:

RefSeq Version	XM_017350980.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nemo-like kinase, type 2 (nlk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017350980.2

ORF Insert Sequence:

ATGGCTCTGT GCGGAACCAC GGCTTCCAAT GCCGCGAAAA TGATGGCTGC GTACAACGGC 
GGCACCGCGG CAGTGGCAGC GCATCACCCG CACCATCACC ACCAGCTGCC ACATCTCCCG 
CCACCCGCCC ACCTCCACCA CCATCACCAC GCTGGCCAGC ATCACCTCCA GCACCCCGGC 
TCGGCCGCCG CCGTGCACCC CGTACAGCAG CACGCTTCTA CGGCCGCGGC CGCTGCTGCT 
GCGGCGGCGG CGGCGGTGAT GCTGAACCCG AGCCAGCAGC AGCCGTACTT CCCCTCGCCC 
GCTCCTGGAC AAGCTCCGGG GCCGGCGGCC GCTGCAGCCC CAGCACAGGT GCAAGCCGCC 
GCCGCGGCCG CTGTAAAGGT GCACCATCAC CACCACCAAC ACTCGCATCA CTTACAACAA 
CAGCTGGATA TAGAGCCGGA CAGACCGATC GGATACGGAG CTTTCGGCGT GGTTTGGTCG 
GTGACAGACC CCAGAGACGG CAAACGCGTG GCACTCAAAA AGATGCCCAA TGTCTTCCAA 
AACCTGGTCT CGTGCAAGAG GGTTTTCCGT GAGCTGAAGA TGCTTTGTTT CTTCAAGCAC 
GACAACGTGC TGTCAGCTCT GGATATTCTG CAGCCTCCAC ACATCGACTA CTTTGAGGAA 
ATTTATGTGG TGACGGAGCT GATGCAGAGC GACCTCCATA AGATCATTGT ATCTCCACAA 
CCTCTGAGCT CGGACCACGT CAAAGTCTTT CTCTACCAGA TCCTTCGTGG ATTGAAGTAT 
CTACACTCAG CCGGCATTTT GCACAGAGAC ATTAAACCTG GGAACCTGCT GGTCAACAGC 
AATTGTGTGC TAAAGATCTG TGACTTTGGC CTGGCGCGGG TGGAGGAGCT GGACGAGTCA 
CGTCACATGA CCCAGGAGGT GGTGACGCAG TACTACAGAG CGCCGGAGAT CCTCATGGGG 
AGCAGACATT ACTCCAACGC CATCGACATC TGGTCTGTGG GCTGCATTTT TGCTGAGCTG 
CTCGGCCGAC GCATCCTTTT CCAGGCCCAG AGTCCCATCC AACAGCTGGA CCTGATCACA 
GATCTGCTAG GGACGCCATC TCTGGAGGCC ATGAGGACGG CCTGTGAGGG GGCCAGAGCT 
CACATTCTCA GAGGACCACA CAAACAGCCC TCACTTCCAG TCCTATATAC CCTGTCCAGT 
CAAGCAACCC ATGAGGCCGT TCACCTCCTC TGCAGGATGT TGGTCTTCGA CCCATCGAAG 
CGAATCTCTG CGAAGGATGC CCTGGCACAC CCGTATCTGG ATGAGGGGCG TCTGCGATAC 
CACACCTGCA TGTGCAAATG CTGCTACACC ACGTCCTCCG GCCGCGTTTA CACCAGTGAC 
TTCGAACCCG TCACAAACCC CAAGTTTGAC GATGGATTTG AGAAGAACCT GACGTCAGTT 
CGGCAGGTCA AAGAGATCAT TCATCAGTTC ATCCTGGAGC AGCAGAAAGG GAATCGAGTA 
CCGCTCTGTA TCAACCCTCA ATCCGCTGCC TTCAAAAGCT TTATCAGCTC AACAGTAGCT 
CAGCCCTCTG AGATGCCTCC ATCTCCTTTA GTTTGGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLK2 ( XM_017350980.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206469.1 Danio rerio nemo-like kinase, type 2 (nlk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55104
Clone ID Related Accession (Same CDS sequence)	XM_017350980.2 , XM_017350980.1
Accession Version	XM_017350980.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase NLK isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT GCGGAACCAC GGCTTCCAAT GCCGCGAAAA TGATGGCTGC GTACAACGGC 
GGCACCGCGG CAGTGGCAGC GCATCACCCG CACCATCACC ACCAGCTGCC ACATCTCCCG 
CCACCCGCCC ACCTCCACCA CCATCACCAC GCTGGCCAGC ATCACCTCCA GCACCCCGGC 
TCGGCCGCCG CCGTGCACCC CGTACAGCAG CACGCTTCTA CGGCCGCGGC CGCTGCTGCT 
GCGGCGGCGG CGGCGGTGAT GCTGAACCCG AGCCAGCAGC AGCCGTACTT CCCCTCGCCC 
GCTCCTGGAC AAGCTCCGGG GCCGGCGGCC GCTGCAGCCC CAGCACAGGT GCAAGCCGCC 
GCCGCGGCCG CTGTAAAGGT GCACCATCAC CACCACCAAC ACTCGCATCA CTTACAACAA 
CAGCTGGATA TAGAGCCGGA CAGACCGATC GGATACGGAG CTTTCGGCGT GGTTTGGTCG 
GTGACAGACC CCAGAGACGG CAAACGCGTG GCACTCAAAA AGATGCCCAA TGTCTTCCAA 
AACCTGGTCT CGTGCAAGAG GGTTTTCCGT GAGCTGAAGA TGCTTTGTTT CTTCAAGCAC 
GACAACGTGC TGTCAGCTCT GGATATTCTG CAGCCTCCAC ACATCGACTA CTTTGAGGAA 
ATTTATGTGG TGACGGAGCT GATGCAGAGC GACCTCCATA AGATCATTGT ATCTCCACAA 
CCTCTGAGCT CGGACCACGT CAAAGTCTTT CTCTACCAGA TCCTTCGTGG ATTGAAGTAT 
CTACACTCAG CCGGCATTTT GCACAGAGAC ATTAAACCTG GGAACCTGCT GGTCAACAGC 
AATTGTGTGC TAAAGATCTG TGACTTTGGC CTGGCGCGGG TGGAGGAGCT GGACGAGTCA 
CGTCACATGA CCCAGGAGGT GGTGACGCAG TACTACAGAG CGCCGGAGAT CCTCATGGGG 
AGCAGACATT ACTCCAACGC CATCGACATC TGGTCTGTGG GCTGCATTTT TGCTGAGCTG 
CTCGGCCGAC GCATCCTTTT CCAGGCCCAG AGTCCCATCC AACAGCTGGA CCTGATCACA 
GATCTGCTAG GGACGCCATC TCTGGAGGCC ATGAGGACGG CCTGTGAGGG GGCCAGAGCT 
CACATTCTCA GAGGACCACA CAAACAGCCC TCACTTCCAG TCCTATATAC CCTGTCCAGT 
CAAGCAACCC ATGAGGCCGT TCACCTCCTC TGCAGGATGT TGGTCTTCGA CCCATCGAAG 
CGAATCTCTG CGAAGGATGC CCTGGCACAC CCGTATCTGG ATGAGGGGCG TCTGCGATAC 
CACACCTGCA TGTGCAAATG CTGCTACACC ACGTCCTCCG GCCGCGTTTA CACCAGTGAC 
TTCGAACCCG TCACAAACCC CAAGTTTGAC GATGGATTTG AGAAGAACCT GACGTCAGTT 
CGGCAGGTCA AAGAGATCAT TCATCAGTTC ATCCTGGAGC AGCAGAAAGG GAATCGAGTA 
CCGCTCTGTA TCAACCCTCA ATCCGCTGCC TTCAAAAGCT TTATCAGCTC AACAGTAGCT 
CAGCCCTCTG AGATGCCTCC ATCTCCTTTA GTTTGGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206469.1
CDS	1255..2856
Translation	MALCGTTASNAAKMMAAYNGGTAAVAAHHPHHHHQLPHLPPPAHLHHHHHAGQHHLQHPGSAAAVHPVQQHASTAAAAAAAAAAAVMLNPSQQQPYFPSPAPGQAPGPAAAAAPAQVQAAAAAAVKVHHHHHQHSHHLQQQLDIEPDRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKICDFGLARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAELLGRRILFQAQSPIQQLDLITDLLGTPSLEAMRTACEGARAHILRGPHKQPSLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRYHTCMCKCCYTTSSGRVYTSDFEPVTNPKFDDGFEKNLTSVRQVKEIIHQFILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE

Target ORF information:

RefSeq Version	XM_017350980.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nemo-like kinase, type 2 (nlk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017350980.1

ORF Insert Sequence:

ATGGCTCTGT GCGGAACCAC GGCTTCCAAT GCCGCGAAAA TGATGGCTGC GTACAACGGC 
GGCACCGCGG CAGTGGCAGC GCATCACCCG CACCATCACC ACCAGCTGCC ACATCTCCCG 
CCACCCGCCC ACCTCCACCA CCATCACCAC GCTGGCCAGC ATCACCTCCA GCACCCCGGC 
TCGGCCGCCG CCGTGCACCC CGTACAGCAG CACGCTTCTA CGGCCGCGGC CGCTGCTGCT 
GCGGCGGCGG CGGCGGTGAT GCTGAACCCG AGCCAGCAGC AGCCGTACTT CCCCTCGCCC 
GCTCCTGGAC AAGCTCCGGG GCCGGCGGCC GCTGCAGCCC CAGCACAGGT GCAAGCCGCC 
GCCGCGGCCG CTGTAAAGGT GCACCATCAC CACCACCAAC ACTCGCATCA CTTACAACAA 
CAGCTGGATA TAGAGCCGGA CAGACCGATC GGATACGGAG CTTTCGGCGT GGTTTGGTCG 
GTGACAGACC CCAGAGACGG CAAACGCGTG GCACTCAAAA AGATGCCCAA TGTCTTCCAA 
AACCTGGTCT CGTGCAAGAG GGTTTTCCGT GAGCTGAAGA TGCTTTGTTT CTTCAAGCAC 
GACAACGTGC TGTCAGCTCT GGATATTCTG CAGCCTCCAC ACATCGACTA CTTTGAGGAA 
ATTTATGTGG TGACGGAGCT GATGCAGAGC GACCTCCATA AGATCATTGT ATCTCCACAA 
CCTCTGAGCT CGGACCACGT CAAAGTCTTT CTCTACCAGA TCCTTCGTGG ATTGAAGTAT 
CTACACTCAG CCGGCATTTT GCACAGAGAC ATTAAACCTG GGAACCTGCT GGTCAACAGC 
AATTGTGTGC TAAAGATCTG TGACTTTGGC CTGGCGCGGG TGGAGGAGCT GGACGAGTCA 
CGTCACATGA CCCAGGAGGT GGTGACGCAG TACTACAGAG CGCCGGAGAT CCTCATGGGG 
AGCAGACATT ACTCCAACGC CATCGACATC TGGTCTGTGG GCTGCATTTT TGCTGAGCTG 
CTCGGCCGAC GCATCCTTTT CCAGGCCCAG AGTCCCATCC AACAGCTGGA CCTGATCACA 
GATCTGCTAG GGACGCCATC TCTGGAGGCC ATGAGGACGG CCTGTGAGGG GGCCAGAGCT 
CACATTCTCA GAGGACCACA CAAACAGCCC TCACTTCCAG TCCTATATAC CCTGTCCAGT 
CAAGCAACCC ATGAGGCCGT TCACCTCCTC TGCAGGATGT TGGTCTTCGA CCCATCGAAG 
CGAATCTCTG CGAAGGATGC CCTGGCACAC CCGTATCTGG ATGAGGGGCG TCTGCGATAC 
CACACCTGCA TGTGCAAATG CTGCTACACC ACGTCCTCCG GCCGCGTTTA CACCAGTGAC 
TTCGAACCCG TCACAAACCC CAAGTTTGAC GATGGATTTG AGAAGAACCT GACGTCAGTT 
CGGCAGGTCA AAGAGATCAT TCATCAGTTC ATCCTGGAGC AGCAGAAAGG GAATCGAGTA 
CCGCTCTGTA TCAACCCTCA ATCCGCTGCC TTCAAAAGCT TTATCAGCTC AACAGTAGCT 
CAGCCCTCTG AGATGCCTCC ATCTCCTTTA GTTTGGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

NLK positively regulates Wnt/?-catenin signalling by phosphorylating LEF1 in neural progenitor cells.
The EMBO journal31(8)1904-15(2012 Apr)
Ota S,Ishitani S,Shimizu N,Matsumoto K,Itoh M,Ishitani T

Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish.
Genome biology8(11)R251(2007)
Stein C,Caccamo M,Laird G,Leptin M