lig3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lig3
Entrez Gene ID	563276
Full Name	ligase III, DNA, ATP-dependent
Synonyms	im:7139055,im:7145225,wu:fb12c02,wu:fd10b03,wu:fm79b09,zgc:112973
General protein information	Preferred Names ligase III, DNA, ATP-dependent Names DNA ligase 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001030174.2	NP_001025345.2	DNA ligase 3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LIG3	NP_001033196.1
Rattus norvegicus (Norway rat)	Lig3	NP_001012011.1
Gallus gallus (chicken)	LIG3	NP_001006215.1
Homo sapiens (human)	LIG3	NP_039269.2
	LIG3	XP_001113780.1
Canis lupus familiaris (dog)	LIG3	XP_005624869.1
Mus musculus (house mouse)	Lig3	NP_034846.2
Drosophila melanogaster (fruit fly)	lig3	NP_650187.2
Xenopus tropicalis (tropical clawed frog)	lig3	NP_001016351.2
Pan troglodytes (chimpanzee)	LIG3	XP_511409.3
Danio rerio (zebrafish)	lig3	NP_001025345.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Polq-Mediated End Joining Is Essential for Surviving DNA Double-Strand Breaks during Early Zebrafish Development.
Thyme SB, Schier AF
Cell reports(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that MMEJ in zebrafish is dependent on ligase 3 but independent of ligase 4.
Title: Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos.

The following lig3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lig3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36554	NM_001030174.2 Latest version!	Danio rerio ligase III, DNA, ATP-dependent (lig3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lig3 ( NM_001030174.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025345.2 Danio rerio ligase III, DNA, ATP-dependent (lig3), mRNA.

Price & Availability↑

CloneID	ODa36554
Clone ID Related Accession (Same CDS sequence)	NM_001030174.2
Accession Version	NM_001030174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	DNA ligase 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU929413.5. On Oct 6, 2012 this sequence version replaced NM_001030174.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GDQH01008025.1, GFIL01014466.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCCCAGTC TTTGGCTTGG CAGGGCATTA AGAACACTCA GCAGATCATC TTTGACCAAA 
ATTTCTTGGG AGCCTTTATT TAGAGCAACA CTCAAACACC AATATCTCCA GTGCTCGAGG 
ACTCTTATAT TTACACGGGG CTCTGAATCA AGCCCCAGTG GAGTCTTTTT CTGTGCGCCA 
CACACTCCAG CATTTACTTT AATATCCAGC TCTTATTTCC ACATTTCCTG GCCTGTGCGG 
AGGAGTGAAC AAGCGGATAT GGCGGAGCAG AGATACTGTG TTGAATATGC CAAACGTGGC 
ACGGCTGGCT GCAAGAAATG CAAAGACAAG ATCATGAAAG GTCTGGTCAG GATCGGCAAG 
ATCGTTCCAA ACCCATTCAG CGAGTCTGCA GGGGAAATGA AGGAATGGTA TCATGTAAAG 
TGTATCTTTG AGAAGCTTGA GAGAGCGCGG GCCACCACAA AGAAGATTGA GGACATCACT 
GAACTGGAGG GATGGGAGGA ACTTCAGGAT GAGGATAAAG ATCTCATCAA TAAACACGTT 
TCAGAACTGG CAGCTAAAGT TAATTCAACC CCAAAGAAGA AAGTGCAAGC AAAGCTAAAC 
ACCAGTGGGC AGCTGTCATC GCCGCCAGCT GATCCAACAG TCAATGCTCC TCGCAAGTTT 
TCTGGCTTCA CAGCTACCAA GGCCGGTTCC TCTTCCTCCA GTCCTGGACC ATCTCCAGCC 
AAACCCGCTC AGGGTAGCGT TTTATCAGCT CAGATGTGTG ACCCCACACA TAAAGACAAC 
TTACTTCGTG AGTTCAGAAA ACTCTGTGCT TTAGTGGCAG AGAAGTCGGG CTACAACGCC 
AAGACGGAGA TCGTCAGAGA CTTCTTAACA AAAGGCTCAG GTGGAGACAA ATTCAGAGGA 
GATTTGTACC TGACAGTGAA GCTTCTCCTC CCAGGCGTAG TGAAGAATGT GTACAACCTC 
AATGACAAAC AAATCGTCAA GCTTTTTAGC CGCATACTGA ATTGCAATCA GGATGACATG 
GTGCAAGACT TGGAACAGGG AGATGTTTCG GAGACGGTGC GGATGTTTTT CGAGGAAAGC 
AAGTCTTTCC CTCCTTCAGC AAAAAGTCTT CTGACCATCC AAGAGGTGGA TGCTTCGCTG 
AGCCGATTAT CCCAGCTCAC AAAGGAGGAC GATCAGCAGG CAGAGCTGCA GAGCATTGCC 
AAAAAATGCA CCGGGAATGA TCTGAAGTGC TACATTCGTT TGGTCAAACA TGATTTGAAA 
ATCAACTCAG GGGCGAAACA CATTTTGGAT GCTGTAGACC CCAATGCCTA TGATGCCTTC 
AAAGCCTCCC GTAATCTCGG TGATGTGATA GACAGAGTGC TGCGTAACCA GCAGGATGCT 
TCTAATGGAA CTGGCCCCCG AAAGATCCTC AGCATCGAGG CATCTCTGAT GACTCCAGTA 
CAGCCAATGC TGGCAGAGGC ATGCAAGTCC ATTGAGTACG CCATGAAGAA GTGTCCTAAT 
GGGATGTATT CTGAGATCAA ATATGATGGT GAGCGCGTGC AGGTCCATAA GAACGGCAAT 
CACTTCAGTT ACTTCAGTCG CAGTCTCAAA CCAGTCCTCC CACACAAGGT TGCCCATTTT 
AAGGACTTCA TACCGCAGGC GTTTGCTGGT GGTCATAGCA TGATCCTTGA TGCTGAAGTT 
CTTCTTATTG ACACAAAGAC TAGCAAACCA CTGCCTTTTG GGACTCTTGG AGTACACAAG 
AAAGCCGCTT TTCAGGATGC TCAAGTGGGC CTGTTCGTGT TCGACTGCAT TTATTTTAAT 
GGTGTCAGTC TAATGGACAA GCCTCTCTGT GAGAGAAGAA AGTTTCTCCA TGATAATATG 
GTGGAAGTTC CTAACCGAAT TCTGTTCTCA GAAATGAAGC ATGTCACAAG AGCTCCTGAT 
CTGGCAGAAA TGATCACGCG TGTCATCAGA GAGGGGCTGG AGGGCTTGGT TCTGAAGGAT 
ATAAAGGGCC TTTATGAACC AGGAAAACGC CACTGGCTGA AGGTGAAGAA AGACTATCTG 
AATGAAGGAG CGATGGCGGA CACTGCTGAT CTTGTGGTGC TTGGAGCCTT CTATGGCAAA 
GGCTCAAATG GTGGAATTAT GTCCAGTTTC CTCATGGGCT GCTATGACCC GGAATCCAGG 
AAGTGGTGCA CCGTTACCAA GTGTTCAGGA GGCTTCGACG ACGCCATGTT AGACAGACTG 
CAGAAAGATT TGGACGTGAT TAAAATTGGC AAGGATCCAA GCAAGATCCC AGGATGGTTG 
AAGATTGTCA AGAACTATTA CCCAGATTTC ATTATCCGAG ATCCACAAAA AGCACCAGTG 
TGGGAAATCA CAGGAGCAGA GTTTTCTAAA TCTGAAATGC ACACTGCTGA TGGCATATCC 
ATCCGATTCC CACGATGCAC CCGAATGCGT GATGACAAGG ACTGGAAGAC GGCAACTAAC 
CTTCAGCAGC TCAAGGAGCT ATATCGCATC TCAAAGGAGA ATTGTGATTT TGAGGTGACC 
GCTGGTCCAT CCAAAGCGAA CCAAAATGAC AAGAGCTCCT CAGGAGGTGA GAGCGGAGGA 
AGCTCTCCAT CTGCATCTCG CGGAGCATCC ACAGCTAACA AACCCAAAAA TGGTCATACC 
CCCAAAGCAA AGGCTGCGAA AACAGAATCA TCTGCTCCGG CAGTTAAGAG GAAACTACCA 
GCTGAGAAAC AAGATGTTAA GAAGATAAAA ACAGAATCGG TTCACAGCAA TGGTCAAAGC 
AAATTGAAGA GCGTTCCAAC AACCGACCCG AAGCAAGAAA AGACACTACT TGACATTTTC 
ACCGGAGTAA AGCTCTACCT CCCTGAATCA GTGAAAGACT TTGAGAAGCT CCGACGCTAC 
TTTCTAGCAT ACGATGGAGA TTTGGTGCCC GAGTATGACT CTGCTTTAGC CACACACACG 
CTAGGACAGC CAGAGGATGG CAGTTCAGCC CAGAAGGTCA CCTCCAACTG GATCTGGGAA 
TGCATCCGGA AAAGGAGGGT GGTTCCCCCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025345.2
CDS	210..3245
Translation	MPSLWLGRALRTLSRSSLTKISWEPLFRATLKHQYLQCSRTLIFTRGSESSPSGVFFCAPHTPAFTLISSSYFHISWPVRRSEQADMAEQRYCVEYAKRGTAGCKKCKDKIMKGLVRIGKIVPNPFSESAGEMKEWYHVKCIFEKLERARATTKKIEDITELEGWEELQDEDKDLINKHVSELAAKVNSTPKKKVQAKLNTSGQLSSPPADPTVNAPRKFSGFTATKAGSSSSSPGPSPAKPAQGSVLSAQMCDPTHKDNLLREFRKLCALVAEKSGYNAKTEIVRDFLTKGSGGDKFRGDLYLTVKLLLPGVVKNVYNLNDKQIVKLFSRILNCNQDDMVQDLEQGDVSETVRMFFEESKSFPPSAKSLLTIQEVDASLSRLSQLTKEDDQQAELQSIAKKCTGNDLKCYIRLVKHDLKINSGAKHILDAVDPNAYDAFKASRNLGDVIDRVLRNQQDASNGTGPRKILSIEASLMTPVQPMLAEACKSIEYAMKKCPNGMYSEIKYDGERVQVHKNGNHFSYFSRSLKPVLPHKVAHFKDFIPQAFAGGHSMILDAEVLLIDTKTSKPLPFGTLGVHKKAAFQDAQVGLFVFDCIYFNGVSLMDKPLCERRKFLHDNMVEVPNRILFSEMKHVTRAPDLAEMITRVIREGLEGLVLKDIKGLYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGKGSNGGIMSSFLMGCYDPESRKWCTVTKCSGGFDDAMLDRLQKDLDVIKIGKDPSKIPGWLKIVKNYYPDFIIRDPQKAPVWEITGAEFSKSEMHTADGISIRFPRCTRMRDDKDWKTATNLQQLKELYRISKENCDFEVTAGPSKANQNDKSSSGGESGGSSPSASRGASTANKPKNGHTPKAKAAKTESSAPAVKRKLPAEKQDVKKIKTESVHSNGQSKLKSVPTTDPKQEKTLLDIFTGVKLYLPESVKDFEKLRRYFLAYDGDLVPEYDSALATHTLGQPEDGSSAQKVTSNWIWECIRKRRVVPPC

Target ORF information:

RefSeq Version	NM_001030174.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ligase III, DNA, ATP-dependent (lig3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030174.2

ORF Insert Sequence:

ATGCCCAGTC TTTGGCTTGG CAGGGCATTA AGAACACTCA GCAGATCATC TTTGACCAAA 
ATTTCTTGGG AGCCTTTATT TAGAGCAACA CTCAAACACC AATATCTCCA GTGCTCGAGG 
ACTCTTATAT TTACACGGGG CTCTGAATCA AGCCCCAGTG GAGTCTTTTT CTGTGCGCCA 
CACACTCCAG CATTTACTTT AATATCCAGC TCTTATTTCC ACATTTCCTG GCCTGTGCGG 
AGGAGTGAAC AAGCGGATAT GGCGGAGCAG AGATACTGTG TTGAATATGC CAAACGTGGC 
ACGGCTGGCT GCAAGAAATG CAAAGACAAG ATCATGAAAG GTCTGGTCAG GATCGGCAAG 
ATCGTTCCAA ACCCATTCAG CGAGTCTGCA GGGGAAATGA AGGAATGGTA TCATGTAAAG 
TGTATCTTTG AGAAGCTTGA GAGAGCGCGG GCCACCACAA AGAAGATTGA GGACATCACT 
GAACTGGAGG GATGGGAGGA ACTTCAGGAT GAGGATAAAG ATCTCATCAA TAAACACGTT 
TCAGAACTGG CAGCTAAAGT TAATTCAACC CCAAAGAAGA AAGTGCAAGC AAAGCTAAAC 
ACCAGTGGGC AGCTGTCATC GCCGCCAGCT GATCCAACAG TCAATGCTCC TCGCAAGTTT 
TCTGGCTTCA CAGCTACCAA GGCCGGTTCC TCTTCCTCCA GTCCTGGACC ATCTCCAGCC 
AAACCCGCTC AGGGTAGCGT TTTATCAGCT CAGATGTGTG ACCCCACACA TAAAGACAAC 
TTACTTCGTG AGTTCAGAAA ACTCTGTGCT TTAGTGGCAG AGAAGTCGGG CTACAACGCC 
AAGACGGAGA TCGTCAGAGA CTTCTTAACA AAAGGCTCAG GTGGAGACAA ATTCAGAGGA 
GATTTGTACC TGACAGTGAA GCTTCTCCTC CCAGGCGTAG TGAAGAATGT GTACAACCTC 
AATGACAAAC AAATCGTCAA GCTTTTTAGC CGCATACTGA ATTGCAATCA GGATGACATG 
GTGCAAGACT TGGAACAGGG AGATGTTTCG GAGACGGTGC GGATGTTTTT CGAGGAAAGC 
AAGTCTTTCC CTCCTTCAGC AAAAAGTCTT CTGACCATCC AAGAGGTGGA TGCTTCGCTG 
AGCCGATTAT CCCAGCTCAC AAAGGAGGAC GATCAGCAGG CAGAGCTGCA GAGCATTGCC 
AAAAAATGCA CCGGGAATGA TCTGAAGTGC TACATTCGTT TGGTCAAACA TGATTTGAAA 
ATCAACTCAG GGGCGAAACA CATTTTGGAT GCTGTAGACC CCAATGCCTA TGATGCCTTC 
AAAGCCTCCC GTAATCTCGG TGATGTGATA GACAGAGTGC TGCGTAACCA GCAGGATGCT 
TCTAATGGAA CTGGCCCCCG AAAGATCCTC AGCATCGAGG CATCTCTGAT GACTCCAGTA 
CAGCCAATGC TGGCAGAGGC ATGCAAGTCC ATTGAGTACG CCATGAAGAA GTGTCCTAAT 
GGGATGTATT CTGAGATCAA ATATGATGGT GAGCGCGTGC AGGTCCATAA GAACGGCAAT 
CACTTCAGTT ACTTCAGTCG CAGTCTCAAA CCAGTCCTCC CACACAAGGT TGCCCATTTT 
AAGGACTTCA TACCGCAGGC GTTTGCTGGT GGTCATAGCA TGATCCTTGA TGCTGAAGTT 
CTTCTTATTG ACACAAAGAC TAGCAAACCA CTGCCTTTTG GGACTCTTGG AGTACACAAG 
AAAGCCGCTT TTCAGGATGC TCAAGTGGGC CTGTTCGTGT TCGACTGCAT TTATTTTAAT 
GGTGTCAGTC TAATGGACAA GCCTCTCTGT GAGAGAAGAA AGTTTCTCCA TGATAATATG 
GTGGAAGTTC CTAACCGAAT TCTGTTCTCA GAAATGAAGC ATGTCACAAG AGCTCCTGAT 
CTGGCAGAAA TGATCACGCG TGTCATCAGA GAGGGGCTGG AGGGCTTGGT TCTGAAGGAT 
ATAAAGGGCC TTTATGAACC AGGAAAACGC CACTGGCTGA AGGTGAAGAA AGACTATCTG 
AATGAAGGAG CGATGGCGGA CACTGCTGAT CTTGTGGTGC TTGGAGCCTT CTATGGCAAA 
GGCTCAAATG GTGGAATTAT GTCCAGTTTC CTCATGGGCT GCTATGACCC GGAATCCAGG 
AAGTGGTGCA CCGTTACCAA GTGTTCAGGA GGCTTCGACG ACGCCATGTT AGACAGACTG 
CAGAAAGATT TGGACGTGAT TAAAATTGGC AAGGATCCAA GCAAGATCCC AGGATGGTTG 
AAGATTGTCA AGAACTATTA CCCAGATTTC ATTATCCGAG ATCCACAAAA AGCACCAGTG 
TGGGAAATCA CAGGAGCAGA GTTTTCTAAA TCTGAAATGC ACACTGCTGA TGGCATATCC 
ATCCGATTCC CACGATGCAC CCGAATGCGT GATGACAAGG ACTGGAAGAC GGCAACTAAC 
CTTCAGCAGC TCAAGGAGCT ATATCGCATC TCAAAGGAGA ATTGTGATTT TGAGGTGACC 
GCTGGTCCAT CCAAAGCGAA CCAAAATGAC AAGAGCTCCT CAGGAGGTGA GAGCGGAGGA 
AGCTCTCCAT CTGCATCTCG CGGAGCATCC ACAGCTAACA AACCCAAAAA TGGTCATACC 
CCCAAAGCAA AGGCTGCGAA AACAGAATCA TCTGCTCCGG CAGTTAAGAG GAAACTACCA 
GCTGAGAAAC AAGATGTTAA GAAGATAAAA ACAGAATCGG TTCACAGCAA TGGTCAAAGC 
AAATTGAAGA GCGTTCCAAC AACCGACCCG AAGCAAGAAA AGACACTACT TGACATTTTC 
ACCGGAGTAA AGCTCTACCT CCCTGAATCA GTGAAAGACT TTGAGAAGCT CCGACGCTAC 
TTTCTAGCAT ACGATGGAGA TTTGGTGCCC GAGTATGACT CTGCTTTAGC CACACACACG 
CTAGGACAGC CAGAGGATGG CAGTTCAGCC CAGAAGGTCA CCTCCAACTG GATCTGGGAA 
TGCATCCGGA AAAGGAGGGT GGTTCCCCCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Polq-Mediated End Joining Is Essential for Surviving DNA Double-Strand Breaks during Early Zebrafish Development.
Cell reports(2016 Apr)
Thyme SB,Schier AF