PLXNB2A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLXNB2A
Entrez Gene ID	561433
Full Name	plexin b2a
Synonyms	fk37b01,im:7054697,plxnB2,si:dkey-261e22.2,wu:fk37b01
General protein information	Preferred Names plexin b2a Names plexin-B2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009300284.3	XP_009298559.1	plexin-B2 isoform X1
XM_009300283.2	XP_009298558.1	plexin-B2 isoform X1
NM_001161600.1	NP_001155072.1	plexin-B2 precursor
XM_009300283.3	XP_009298558.1	plexin-B2 isoform X1
XM_009300284.2	XP_009298559.1	plexin-B2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PLXNB2	NP_036533.2
	PLXNB2	XP_001116282.2
Danio rerio (zebrafish)	plxnb2a	NP_001155072.1
Pan troglodytes (chimpanzee)	PLXNB2	XP_003953967.1
Canis lupus familiaris (dog)	PLXNB2	XP_531689.3
Danio rerio (zebrafish)	plxnb2b	NP_001184245.1
Bos taurus (cattle)	PLXNB2	NP_001192558.1
Mus musculus (house mouse)	Plxnb2	NP_001152993.1
Rattus norvegicus (Norway rat)	Plxnb2	NP_001101576.1
Gallus gallus (chicken)	PLXNB2	XP_423623.4

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Novel blood collection method allows plasma proteome analysis from single zebrafish.
Babaei F, Ramalingam R, Tavendale A, Liang Y, Yan LS, Ajuh P, Cheng SH, Lam YW
Journal of proteome research12(4)1580-90(2013 Apr)

Conservation, expression, and knockdown of zebrafish plxnb2a and plxnb2b.
Per?l? N, Peitsaro N, Sundvik M, Koivula H, Sainio K, Sariola H, Panula P, Immonen T
Developmental dynamics : an official publication of the American Association of Anatomists239(10)2722-34(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of both plxnb2a and plxnb2b simultaneously (>95% and 45%, respectively) resulted in normal CNS structure, axon guidance and swimming performance of the morphants.
Title: Conservation, expression, and knockdown of zebrafish plxnb2a and plxnb2b.

Loss of plxnB2 results in delayed ISV sprouting identical to that seen in sema3e morphants, while loss of plexinD1 in out of bounds (obd) mutants results in precocious ISV sprouting.
Title: Antagonistic interactions among Plexins regulate the timing of intersegmental vessel formation.

The following PLXNB2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLXNB2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00862	NM_001161600.1 Latest version!	Danio rerio plexin b2a (plxnb2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43506	XM_009300283.3 Latest version!	Danio rerio plexin b2a (plxnb2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43506	XM_009300284.3 Latest version!	Danio rerio plexin b2a (plxnb2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa43506	XM_009300283.2	Danio rerio plexin b2a (plxnb2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43506	XM_009300284.2	Danio rerio plexin b2a (plxnb2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLXNB2A ( NM_001161600.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001155072.1 Danio rerio plexin b2a (plxnb2a), mRNA.

Price & Availability↑

CloneID	ODa00862
Clone ID Related Accession (Same CDS sequence)	NM_001161600.1
Accession Version	NM_001161600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	plexin-B2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU743740.1. On Jun 7, 2009 this sequence version replaced XM_684838.3. ##Evidence-Data-START## Transcript exon combination :: EU743740.1, FJ480176.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTGGA GGGCCCTGTC AGCTTTCTCT CTGCTGCTGT GTTTTGGCTC ATCTTGCTGT 
GATGATGCTC AAGAACAGTT CTCGTCTCCT ACTATCATCA ATAATGTGGT CCAGGACCCT 
CAAACAGGCC GAATTTACCT TGGAGCTGTC AATGGGATCT TTCAGCTGAA CCACAATCTA 
CAGGAGGAGG GCAGAGCAGA CACAGGCCCT AAGAAAGACA ACCCACAATG CACTCCACCT 
ATCACTGCGC AGTGCACCGA TGCGAAGGAA ATGGACAACT TTAACAAACT CCTTCTGGTT 
AACTCCGCCA ACGGCACCTT GATCGTTTGT GGAAGCCTTT TCAGGGGCAT CTGCTCTTTG 
GTAAACCTCA ACAGTGTGAA TAAACCGGTG TACTACAGTG ACACCAAAGG AGAGAAGACA 
TATGTTGCCA GCACTGAGGA ATCTGTCACT GTGGTGGGGG TCATTTCTTA TTTTAAGGAC 
ACTACTACTA ATGCTAACCT CAGTGTATTT CTAGTGGGAA AAGGCTATGG AAGTTCGGAC 
AGCTCAAAGC TCATTAGCAC TCGGTTGCTG CAGGAACATG GAGAAATGGA TGTTTTTGAG 
AACATGGTTG AAGCTTCCAC TGTTCAAACG AGTGCGTTTG TCCTTCGGTA CCACCACGAT 
TTTCGCTACA CGTTTAAAGA CAATGGCTAC ATCTACTTCC TGTTCTCCCG CTCTCCTGGC 
ACTAGCGACA GCACGAAGAT TACATTTATA GCACGCCTTT GCGAGAACGA CCATCATTAT 
TATTCTTACA CAGAACTTCA GCTCAACTGC ACTGTTAACA CCGAACAACA AGGGAACACA 
TTCAACAAAG TCCAAGCAGC GTATCTGGCT AAACCTGGAA AGGATTTGGC TCAGAATATC 
GTCCCGTCAA ATTTAAATGA TAAGGTTCTC TTTGGAGTGT TCAGTGCAGA TGAGGCGGAC 
GGGCGTTCAG CATTGTGCAT GTACCCGCTT AGCAGCATTA ATGCCAGGTT TGAGGAGGTG 
ATTGAGTCCT GCTACACAGG AGAAGTTCTG GAGGATGACA AACCGAAGAC CGTTTATTCA 
CCCTACAACT CCAAGAATGA AGCCATATGC AAGACCAAAA GAGATAAGAA CATGGTAAAG 
GCATATATAT GTGGCGCAGA GTTTCTGCCA TCTCCACTAG CCAGTAAACC TGAGTATGCT 
TTAGCCGTCA AACCCATCTA CACCAGGAAT GACATGCTGA CAGCTGTAGC TGTGGCGGTG 
GAGAACGAAC ACACTGTGGC ATTCTTGGGA ACCAGTGGTG CAGATGTCTT GAAGGTGCAT 
CTCGACCCAA ACCACACTGA CTTTTACAAC AGAATTCCAG GGGACCGTGC AGATGGTGCA 
GTAAACAAAA ACCTTTTGTT TGATTCAACT TTGAATCACC TGTACATCAC CACTGGCAAA 
AAGATCTCCA AAGTGTCAGT GCAGGCCTGT GAGCAGAGAG AAGATTGTCA CTCATGTGTT 
TCCCAGCGGG ACCCATATTG CGGCTGGTGT GTGTTGGAGG GCCGCTGCAC CAGGAAAAAG 
GATTGTCGAA AAGGAGATGG AGAAAACGCC TGGTTGTGGA GTCCAAAACA AACATGTGTA 
ACCATTCAGT CCTTTGAGCC CCCGAACATC AGCTGCAAGA AAACAGATAG TACACAGGTG 
GTGATCAACA TCCCGTCCCT CCCGAGCATC AGCAGTCCTG ACAGGCTGGA GTGTGTGTTT 
GACGGCTACA GGAGTCAAGC AAAGATGGAG GACACTCGGG TGACCTGCTA CCTCCCCAGC 
TCGGACTACA TCCCACCAAC ACCCGAGACA CAAGATTTTG TGGCAGTGCC AGTAAAAATC 
ACAGTGAATG ACAAGATTGA GGTGGCATCG AGTGTGTTTG AGTTTTACAA CTGTGCCGGC 
GTGGTGAAAA AATCAGAGAA TACACCATGC ATTGCATGTG TGACCAGTAA GTGGGGCTGT 
CAGTGGAATA TTCAGGATCA TACATGCAGT GATAAAGACG ACTCTGCCCA TGGGAGTCAT 
ATCATCGGAA ACATGGAGGC TAATCTGTGT CCACAGTTTG AGATTCCTGA GCCTCTGCGT 
ATTCCTGTTG GTTATAAAAC CCCCATCACA TTTCATGGCA AAAATCTGGA TATTTATTGG 
GGCAAAACCT TTCAGATCGG CACCGAGCTC ATGAAGCAGG TGGGCGAAGT GACTGTGGAG 
GCCGATGGGT TAAAGTACAG ATTTGAGGGA TATGAGTTTG CGTATGACAA AGAGCCAGAG 
GTGAATGTGA CGTTCTACAT AAAGGATAAA GACACGGATA GGAAGATTGA CAGCACCCTG 
AGAGTGGTTC TCTACAACTG TTCGGTTAGA CGCGAGGACT GCAGTCTGTG TAAAAATGCA 
CACAAGAATG ATAGCTGCGT TTGGTGCGAC ACTACAAAAT CCTGCATCTA CAGAAGCCTG 
TGTGACAATG AACTGCACCA GTGTCCTCCT CCAAAGATCA CAGACATTGT TCCCCGTTAT 
GGGCCAATGA ACGGACGCAT CTCAGTCACA ATAAAAGGCT CAAATTTGGG CATCAAGAAG 
GAGGACATTA AGAAAATCAC TGTGGCTAAA GTTCCATGCA TTCATCAGCC AGAGCGATAC 
TCGGTTTCTA CTAGTGTTGT GTGTGAGATC GGCCCTTTCC CCTCACCTCC TGAAGATCTG 
CCCATTTCAC CGAGCAAATC CGGGCCGGTG GAGGTGTTGG TGGAAGGAGA AAGAACAGGG 
ACGTCCGAGG TGGAATTCAC TTACAGGGAT CCAGTCCCAG AAGCTGTGTT CCCCGTTAAA 
GGCCCAGCAG CTGGAGGAAC TGTCATCACA ATCACAGGAC AGGACCTCGA CTCGGCCAGT 
AAAGATGACA TCACCATTAC AGTGGACAAA GAGCCATGTG AAGTGTTGAC GTTTGGCCAT 
GAGATCACCT GCAGGACTGG CCCATATAAA GGACAAAAGA TTCCCTCGGA TAAGTTGCCT 
GTTATTGTGA AGTATGGCAA GGACACTGTT AAGACAGTCG ATGCATTCGA ATACTCACCC 
GATCCCAAAA TTTCTGAATA TTTTCCACAG ACCAGCTTTG TTTGCGGTGG TCGCAGGATC 
TTAGTGATAG GCTCTGGATT TGATCAGGTT CAGAGAGCCA CCATGCGAGT GCTGCCCTCT 
TCTGACGAGT TTGACGTCAA GGCTAGCAAT GTAGAGTTCT TTCAAGAAGT GGAAAAGAAG 
AACGAGACGA CGCTGGAGTT TCTGTCGCCG GTTGTCAATA AGACATACAT CAACCTGTCC 
TTAAGAACCG TCATCCAGCT GGACAACCTG GTGCTCAATC TGACAGATTT CGCTTACCAT 
CCGGACCCAC ACTTCAATAA ACTCAAAGAT AAAGCCATAA CAGAGGGCAG CATCATCAAT 
GTTAATGGTA GAGGTTTTGC CAAAGCCATG ACTGCCAAAG AGGCACAGGC ATTTGTGGGT 
GACGAGTCGT GTCTGGTGAC AATCCTTGAG GACGATAAAC TGTTCCTGGT GGCCCCGTCC 
AGCCCTCCAA AATCCACCTC CAGACGCCAT CGAAGAGACA CCAGCTCAGA TGCCTTGGAG 
CTGCTGATCA AGTTTGGAAA TGGAGAGTGG AATGTAGGCT CCGTGCGCTT CGAGAGGAAA 
TACGAGGTTC CCCTTTACAT CATAATCCCC GCTGTTCTCA TTCCCATGGT GCTCTTCATT 
GCGGTGTCCA TCTATTGCTA CAGGAGGAAA AGCCAGCAGG CAGAACGAGA ATATGAGAAG 
ATCAAAAATC AGCTGGCCAA TTTGGAGGAG AGCGTCCGTG ATCGCTGTAC AAAGGAGTTC 
ACAGATCTAA TGATAGAGAT GGAGGATCAC ACCAGTGATC TGAGTGAAGC TCGCATCCCA 
TTCCTGGACT ACAAGAACTA CACCGACCGT GTTTTCTTCC TGCCCTCGAA AGATGGAGCC 
AATGATGTCA TGATCACCGG CAAGCTGGAT ATCCCTGATA ACCGCAGAGC CATCGTAGAT 
CAGGCACTCA ACCAGTTCTC CAACCTCCTG AACAGCAAAA CCTTCCTTAT CAATTTTATT 
CGTACCCTGG AGGGCCGACA GGAGTTCAAT GCCCGGAATA AGGTTTACTT CGCCTCTTTG 
TTGACGGTGG CACTGCATGG CAAGTTGGAG TATTACACGG ACATCATGAG GACTCTCCTG 
CTGGAGCTCA TGGAGGAATA TGTTCACAGC AAGAATCCCA AACTCATGCT CCGCAGATCC 
GAGACTGTTG TGGAGAGGAT GTTGTGCAAC TGGATGTCGA TTTGTCTCTA CCAGTTCCTG 
AAGGACTCAG CCGGCGAGCC GCTTTATAAG CTCTTTAAGG CCATTAAACA CCAGGTGGAA 
AAAGGCCCTG TTGATGCCAA GATGAAGAAA GCCAAGTACA CTCTCAACGA CACGGGGCTT 
CTGGGAGATG ATGTTGAGTA CAGCATTTTG ACCCTACAGG TGCTGGTTCA TGGCGAGGGT 
CCGGACGTGA CCCCGGTCAA AGTGCTAAAC TGCGACACCA TCTCGCAGGT GAAGGAGAAG 
ATCATCGAGC AGGTGTACAG GAATCTGCCA TATTCCCAGA GGCCCGTGGT GGACAGCGTC 
GCACTGGAGT GGCGTCCTGG TTCTACAGGG CAGATTCTGT CTGATCTGGA CCTGACCTCA 
CAGAAGGAGG GAAGGTGGAA ACGGATCAAC ACACTGGCAC ACTATAATGT GCGAGACGGA 
GCCACCCTGG TCTTATCCAG AGTCCTGCAC ACTCAGCAGT CCTTCGACCA GAACATGGAC 
AACCATGAAG AAAGAAATGC TTTGCTGGAG GATGATAAAG TGTTTCACTT GGTGAGACCG 
GCGGATGAGC TGGAGGAGGT CAAATCTAAG AGAGGGAGCA TGAAGGACAA GGCCACCACA 
AAAGCCATCA CTGAGATCTA CCTGACCAGA CTGCTCTCCA TGAAGGGAAC GCTGCAGCAG 
TTTGTAGATG ATTTCTTCCG CAGTGTGTTG TGTTCAGGGA CTGTGGTTCC TCCAGCCGTC 
AAGTACTTCT TTGACTTCTT AGATGAACAA GCTCAAAAAC ATGACAACGT TGATGATGAG 
ACCATCCACA TATGGAAGAC CAACAGTCTT CCTCTCCGTT TCTGGGTGAA CATCTTGAAG 
AATCCTCACT TCATCTTCGA TGTTCACCTG ACTGAGGTAG TGGACGCATC TCTGTCCGTC 
ATCGCTCAGA CCTTCATGGA CGCCTGCACT AAAACCGAGC ACAAACTGAC GCGGGAGTCT 
CCCAGTAACA AACTGCTTTA TGCAAAGGAA ATCTCCACGT ACAAGAAGAT GGTTGATGAT 
TATTATAAAG GCATTCGGCA GATGGTTCCA GTCAGTGACC AAGACATGAA CACACACCTC 
GCAGAGATTT CACGGTCTCA CACAGATAAG TTGAACACCC AGGCCGCGCT GCACCAGCTC 
TACCAGTATG CCAGCAAGTA CTATGACGGG ATCATCTCGT CACTGGAGGA AGATCCTGCC 
GCCCAGAGCA AACAACTGAC CCTCAGACTG CAACAGATCG CCGCTGCGCT GGAGAATAAA 
GTCACCGACC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155072.1
CDS	1..5595
Translation	MAWRALSAFSLLLCFGSSCCDDAQEQFSSPTIINNVVQDPQTGRIYLGAVNGIFQLNHNLQEEGRADTGPKKDNPQCTPPITAQCTDAKEMDNFNKLLLVNSANGTLIVCGSLFRGICSLVNLNSVNKPVYYSDTKGEKTYVASTEESVTVVGVISYFKDTTTNANLSVFLVGKGYGSSDSSKLISTRLLQEHGEMDVFENMVEASTVQTSAFVLRYHHDFRYTFKDNGYIYFLFSRSPGTSDSTKITFIARLCENDHHYYSYTELQLNCTVNTEQQGNTFNKVQAAYLAKPGKDLAQNIVPSNLNDKVLFGVFSADEADGRSALCMYPLSSINARFEEVIESCYTGEVLEDDKPKTVYSPYNSKNEAICKTKRDKNMVKAYICGAEFLPSPLASKPEYALAVKPIYTRNDMLTAVAVAVENEHTVAFLGTSGADVLKVHLDPNHTDFYNRIPGDRADGAVNKNLLFDSTLNHLYITTGKKISKVSVQACEQREDCHSCVSQRDPYCGWCVLEGRCTRKKDCRKGDGENAWLWSPKQTCVTIQSFEPPNISCKKTDSTQVVINIPSLPSISSPDRLECVFDGYRSQAKMEDTRVTCYLPSSDYIPPTPETQDFVAVPVKITVNDKIEVASSVFEFYNCAGVVKKSENTPCIACVTSKWGCQWNIQDHTCSDKDDSAHGSHIIGNMEANLCPQFEIPEPLRIPVGYKTPITFHGKNLDIYWGKTFQIGTELMKQVGEVTVEADGLKYRFEGYEFAYDKEPEVNVTFYIKDKDTDRKIDSTLRVVLYNCSVRREDCSLCKNAHKNDSCVWCDTTKSCIYRSLCDNELHQCPPPKITDIVPRYGPMNGRISVTIKGSNLGIKKEDIKKITVAKVPCIHQPERYSVSTSVVCEIGPFPSPPEDLPISPSKSGPVEVLVEGERTGTSEVEFTYRDPVPEAVFPVKGPAAGGTVITITGQDLDSASKDDITITVDKEPCEVLTFGHEITCRTGPYKGQKIPSDKLPVIVKYGKDTVKTVDAFEYSPDPKISEYFPQTSFVCGGRRILVIGSGFDQVQRATMRVLPSSDEFDVKASNVEFFQEVEKKNETTLEFLSPVVNKTYINLSLRTVIQLDNLVLNLTDFAYHPDPHFNKLKDKAITEGSIINVNGRGFAKAMTAKEAQAFVGDESCLVTILEDDKLFLVAPSSPPKSTSRRHRRDTSSDALELLIKFGNGEWNVGSVRFERKYEVPLYIIIPAVLIPMVLFIAVSIYCYRRKSQQAEREYEKIKNQLANLEESVRDRCTKEFTDLMIEMEDHTSDLSEARIPFLDYKNYTDRVFFLPSKDGANDVMITGKLDIPDNRRAIVDQALNQFSNLLNSKTFLINFIRTLEGRQEFNARNKVYFASLLTVALHGKLEYYTDIMRTLLLELMEEYVHSKNPKLMLRRSETVVERMLCNWMSICLYQFLKDSAGEPLYKLFKAIKHQVEKGPVDAKMKKAKYTLNDTGLLGDDVEYSILTLQVLVHGEGPDVTPVKVLNCDTISQVKEKIIEQVYRNLPYSQRPVVDSVALEWRPGSTGQILSDLDLTSQKEGRWKRINTLAHYNVRDGATLVLSRVLHTQQSFDQNMDNHEERNALLEDDKVFHLVRPADELEEVKSKRGSMKDKATTKAITEIYLTRLLSMKGTLQQFVDDFFRSVLCSGTVVPPAVKYFFDFLDEQAQKHDNVDDETIHIWKTNSLPLRFWVNILKNPHFIFDVHLTEVVDASLSVIAQTFMDACTKTEHKLTRESPSNKLLYAKEISTYKKMVDDYYKGIRQMVPVSDQDMNTHLAEISRSHTDKLNTQAALHQLYQYASKYYDGIISSLEEDPAAQSKQLTLRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	NM_001161600.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio plexin b2a (plxnb2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161600.1

ORF Insert Sequence:

ATGGCGTGGA GGGCCCTGTC AGCTTTCTCT CTGCTGCTGT GTTTTGGCTC ATCTTGCTGT 
GATGATGCTC AAGAACAGTT CTCGTCTCCT ACTATCATCA ATAATGTGGT CCAGGACCCT 
CAAACAGGCC GAATTTACCT TGGAGCTGTC AATGGGATCT TTCAGCTGAA CCACAATCTA 
CAGGAGGAGG GCAGAGCAGA CACAGGCCCT AAGAAAGACA ACCCACAATG CACTCCACCT 
ATCACTGCGC AGTGCACCGA TGCGAAGGAA ATGGACAACT TTAACAAACT CCTTCTGGTT 
AACTCCGCCA ACGGCACCTT GATCGTTTGT GGAAGCCTTT TCAGGGGCAT CTGCTCTTTG 
GTAAACCTCA ACAGTGTGAA TAAACCGGTG TACTACAGTG ACACCAAAGG AGAGAAGACA 
TATGTTGCCA GCACTGAGGA ATCTGTCACT GTGGTGGGGG TCATTTCTTA TTTTAAGGAC 
ACTACTACTA ATGCTAACCT CAGTGTATTT CTAGTGGGAA AAGGCTATGG AAGTTCGGAC 
AGCTCAAAGC TCATTAGCAC TCGGTTGCTG CAGGAACATG GAGAAATGGA TGTTTTTGAG 
AACATGGTTG AAGCTTCCAC TGTTCAAACG AGTGCGTTTG TCCTTCGGTA CCACCACGAT 
TTTCGCTACA CGTTTAAAGA CAATGGCTAC ATCTACTTCC TGTTCTCCCG CTCTCCTGGC 
ACTAGCGACA GCACGAAGAT TACATTTATA GCACGCCTTT GCGAGAACGA CCATCATTAT 
TATTCTTACA CAGAACTTCA GCTCAACTGC ACTGTTAACA CCGAACAACA AGGGAACACA 
TTCAACAAAG TCCAAGCAGC GTATCTGGCT AAACCTGGAA AGGATTTGGC TCAGAATATC 
GTCCCGTCAA ATTTAAATGA TAAGGTTCTC TTTGGAGTGT TCAGTGCAGA TGAGGCGGAC 
GGGCGTTCAG CATTGTGCAT GTACCCGCTT AGCAGCATTA ATGCCAGGTT TGAGGAGGTG 
ATTGAGTCCT GCTACACAGG AGAAGTTCTG GAGGATGACA AACCGAAGAC CGTTTATTCA 
CCCTACAACT CCAAGAATGA AGCCATATGC AAGACCAAAA GAGATAAGAA CATGGTAAAG 
GCATATATAT GTGGCGCAGA GTTTCTGCCA TCTCCACTAG CCAGTAAACC TGAGTATGCT 
TTAGCCGTCA AACCCATCTA CACCAGGAAT GACATGCTGA CAGCTGTAGC TGTGGCGGTG 
GAGAACGAAC ACACTGTGGC ATTCTTGGGA ACCAGTGGTG CAGATGTCTT GAAGGTGCAT 
CTCGACCCAA ACCACACTGA CTTTTACAAC AGAATTCCAG GGGACCGTGC AGATGGTGCA 
GTAAACAAAA ACCTTTTGTT TGATTCAACT TTGAATCACC TGTACATCAC CACTGGCAAA 
AAGATCTCCA AAGTGTCAGT GCAGGCCTGT GAGCAGAGAG AAGATTGTCA CTCATGTGTT 
TCCCAGCGGG ACCCATATTG CGGCTGGTGT GTGTTGGAGG GCCGCTGCAC CAGGAAAAAG 
GATTGTCGAA AAGGAGATGG AGAAAACGCC TGGTTGTGGA GTCCAAAACA AACATGTGTA 
ACCATTCAGT CCTTTGAGCC CCCGAACATC AGCTGCAAGA AAACAGATAG TACACAGGTG 
GTGATCAACA TCCCGTCCCT CCCGAGCATC AGCAGTCCTG ACAGGCTGGA GTGTGTGTTT 
GACGGCTACA GGAGTCAAGC AAAGATGGAG GACACTCGGG TGACCTGCTA CCTCCCCAGC 
TCGGACTACA TCCCACCAAC ACCCGAGACA CAAGATTTTG TGGCAGTGCC AGTAAAAATC 
ACAGTGAATG ACAAGATTGA GGTGGCATCG AGTGTGTTTG AGTTTTACAA CTGTGCCGGC 
GTGGTGAAAA AATCAGAGAA TACACCATGC ATTGCATGTG TGACCAGTAA GTGGGGCTGT 
CAGTGGAATA TTCAGGATCA TACATGCAGT GATAAAGACG ACTCTGCCCA TGGGAGTCAT 
ATCATCGGAA ACATGGAGGC TAATCTGTGT CCACAGTTTG AGATTCCTGA GCCTCTGCGT 
ATTCCTGTTG GTTATAAAAC CCCCATCACA TTTCATGGCA AAAATCTGGA TATTTATTGG 
GGCAAAACCT TTCAGATCGG CACCGAGCTC ATGAAGCAGG TGGGCGAAGT GACTGTGGAG 
GCCGATGGGT TAAAGTACAG ATTTGAGGGA TATGAGTTTG CGTATGACAA AGAGCCAGAG 
GTGAATGTGA CGTTCTACAT AAAGGATAAA GACACGGATA GGAAGATTGA CAGCACCCTG 
AGAGTGGTTC TCTACAACTG TTCGGTTAGA CGCGAGGACT GCAGTCTGTG TAAAAATGCA 
CACAAGAATG ATAGCTGCGT TTGGTGCGAC ACTACAAAAT CCTGCATCTA CAGAAGCCTG 
TGTGACAATG AACTGCACCA GTGTCCTCCT CCAAAGATCA CAGACATTGT TCCCCGTTAT 
GGGCCAATGA ACGGACGCAT CTCAGTCACA ATAAAAGGCT CAAATTTGGG CATCAAGAAG 
GAGGACATTA AGAAAATCAC TGTGGCTAAA GTTCCATGCA TTCATCAGCC AGAGCGATAC 
TCGGTTTCTA CTAGTGTTGT GTGTGAGATC GGCCCTTTCC CCTCACCTCC TGAAGATCTG 
CCCATTTCAC CGAGCAAATC CGGGCCGGTG GAGGTGTTGG TGGAAGGAGA AAGAACAGGG 
ACGTCCGAGG TGGAATTCAC TTACAGGGAT CCAGTCCCAG AAGCTGTGTT CCCCGTTAAA 
GGCCCAGCAG CTGGAGGAAC TGTCATCACA ATCACAGGAC AGGACCTCGA CTCGGCCAGT 
AAAGATGACA TCACCATTAC AGTGGACAAA GAGCCATGTG AAGTGTTGAC GTTTGGCCAT 
GAGATCACCT GCAGGACTGG CCCATATAAA GGACAAAAGA TTCCCTCGGA TAAGTTGCCT 
GTTATTGTGA AGTATGGCAA GGACACTGTT AAGACAGTCG ATGCATTCGA ATACTCACCC 
GATCCCAAAA TTTCTGAATA TTTTCCACAG ACCAGCTTTG TTTGCGGTGG TCGCAGGATC 
TTAGTGATAG GCTCTGGATT TGATCAGGTT CAGAGAGCCA CCATGCGAGT GCTGCCCTCT 
TCTGACGAGT TTGACGTCAA GGCTAGCAAT GTAGAGTTCT TTCAAGAAGT GGAAAAGAAG 
AACGAGACGA CGCTGGAGTT TCTGTCGCCG GTTGTCAATA AGACATACAT CAACCTGTCC 
TTAAGAACCG TCATCCAGCT GGACAACCTG GTGCTCAATC TGACAGATTT CGCTTACCAT 
CCGGACCCAC ACTTCAATAA ACTCAAAGAT AAAGCCATAA CAGAGGGCAG CATCATCAAT 
GTTAATGGTA GAGGTTTTGC CAAAGCCATG ACTGCCAAAG AGGCACAGGC ATTTGTGGGT 
GACGAGTCGT GTCTGGTGAC AATCCTTGAG GACGATAAAC TGTTCCTGGT GGCCCCGTCC 
AGCCCTCCAA AATCCACCTC CAGACGCCAT CGAAGAGACA CCAGCTCAGA TGCCTTGGAG 
CTGCTGATCA AGTTTGGAAA TGGAGAGTGG AATGTAGGCT CCGTGCGCTT CGAGAGGAAA 
TACGAGGTTC CCCTTTACAT CATAATCCCC GCTGTTCTCA TTCCCATGGT GCTCTTCATT 
GCGGTGTCCA TCTATTGCTA CAGGAGGAAA AGCCAGCAGG CAGAACGAGA ATATGAGAAG 
ATCAAAAATC AGCTGGCCAA TTTGGAGGAG AGCGTCCGTG ATCGCTGTAC AAAGGAGTTC 
ACAGATCTAA TGATAGAGAT GGAGGATCAC ACCAGTGATC TGAGTGAAGC TCGCATCCCA 
TTCCTGGACT ACAAGAACTA CACCGACCGT GTTTTCTTCC TGCCCTCGAA AGATGGAGCC 
AATGATGTCA TGATCACCGG CAAGCTGGAT ATCCCTGATA ACCGCAGAGC CATCGTAGAT 
CAGGCACTCA ACCAGTTCTC CAACCTCCTG AACAGCAAAA CCTTCCTTAT CAATTTTATT 
CGTACCCTGG AGGGCCGACA GGAGTTCAAT GCCCGGAATA AGGTTTACTT CGCCTCTTTG 
TTGACGGTGG CACTGCATGG CAAGTTGGAG TATTACACGG ACATCATGAG GACTCTCCTG 
CTGGAGCTCA TGGAGGAATA TGTTCACAGC AAGAATCCCA AACTCATGCT CCGCAGATCC 
GAGACTGTTG TGGAGAGGAT GTTGTGCAAC TGGATGTCGA TTTGTCTCTA CCAGTTCCTG 
AAGGACTCAG CCGGCGAGCC GCTTTATAAG CTCTTTAAGG CCATTAAACA CCAGGTGGAA 
AAAGGCCCTG TTGATGCCAA GATGAAGAAA GCCAAGTACA CTCTCAACGA CACGGGGCTT 
CTGGGAGATG ATGTTGAGTA CAGCATTTTG ACCCTACAGG TGCTGGTTCA TGGCGAGGGT 
CCGGACGTGA CCCCGGTCAA AGTGCTAAAC TGCGACACCA TCTCGCAGGT GAAGGAGAAG 
ATCATCGAGC AGGTGTACAG GAATCTGCCA TATTCCCAGA GGCCCGTGGT GGACAGCGTC 
GCACTGGAGT GGCGTCCTGG TTCTACAGGG CAGATTCTGT CTGATCTGGA CCTGACCTCA 
CAGAAGGAGG GAAGGTGGAA ACGGATCAAC ACACTGGCAC ACTATAATGT GCGAGACGGA 
GCCACCCTGG TCTTATCCAG AGTCCTGCAC ACTCAGCAGT CCTTCGACCA GAACATGGAC 
AACCATGAAG AAAGAAATGC TTTGCTGGAG GATGATAAAG TGTTTCACTT GGTGAGACCG 
GCGGATGAGC TGGAGGAGGT CAAATCTAAG AGAGGGAGCA TGAAGGACAA GGCCACCACA 
AAAGCCATCA CTGAGATCTA CCTGACCAGA CTGCTCTCCA TGAAGGGAAC GCTGCAGCAG 
TTTGTAGATG ATTTCTTCCG CAGTGTGTTG TGTTCAGGGA CTGTGGTTCC TCCAGCCGTC 
AAGTACTTCT TTGACTTCTT AGATGAACAA GCTCAAAAAC ATGACAACGT TGATGATGAG 
ACCATCCACA TATGGAAGAC CAACAGTCTT CCTCTCCGTT TCTGGGTGAA CATCTTGAAG 
AATCCTCACT TCATCTTCGA TGTTCACCTG ACTGAGGTAG TGGACGCATC TCTGTCCGTC 
ATCGCTCAGA CCTTCATGGA CGCCTGCACT AAAACCGAGC ACAAACTGAC GCGGGAGTCT 
CCCAGTAACA AACTGCTTTA TGCAAAGGAA ATCTCCACGT ACAAGAAGAT GGTTGATGAT 
TATTATAAAG GCATTCGGCA GATGGTTCCA GTCAGTGACC AAGACATGAA CACACACCTC 
GCAGAGATTT CACGGTCTCA CACAGATAAG TTGAACACCC AGGCCGCGCT GCACCAGCTC 
TACCAGTATG CCAGCAAGTA CTATGACGGG ATCATCTCGT CACTGGAGGA AGATCCTGCC 
GCCCAGAGCA AACAACTGAC CCTCAGACTG CAACAGATCG CCGCTGCGCT GGAGAATAAA 
GTCACCGACC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLXNB2A ( XM_009300284.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298559.1 Danio rerio plexin b2a (plxnb2a), transcript variant X2, mRNA.

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CloneID	ODa43506
Clone ID Related Accession (Same CDS sequence)	XM_009300284.3 , XM_009300283.2 , XM_009300283.3 , XM_009300284.2
Accession Version	XM_009300284.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plexin-B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300284.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTGGA GGGCCCTGGC AGCTTTCTCT CTACTACTGT GTTTTGGCTC ATCTTGCTGT 
GATGATGCTC AAGAACAGTT CTCGTCTCCT ACCATCATCA ATAATGTGGT CCAGGACCCT 
CAAACAGGCC GAATCTACCT TGGAGCTGTC AATGGGATCT TTCAGCTGAA CCACAATCTA 
CAGGAGGAGA GCAGAGCAGA CACAGGCCCT AAGAAAGACA ACCCACAATG CACTCCACCT 
ATCACTGCGC AGTGCACCGA TGCGAAGGAA ATGGACAACT TTAACAAACT CCTTCTGGTT 
AACTCCGCCA ACGGCACCTT GATCGTTTGT GGAAGCCTTT TCAGGGGCAT CTGCTCTTTG 
GTAAACCTCA ACAGTGTGAA TAAACCGGTG TACTACAGTG ACACCAAAGG AGAGAAGACA 
TATGTTGCCA GCACTGAGGA ATCTGTCACT GTGGTGGGGG TCATTTCTTA TTTTAAGGAC 
ACTACTACTA ATGCTAACCT CAGTGTATTT CTAGTGGGAA AAGGCTATGG AAGTTCGGAC 
AGCTCAAAGC TCATTAGCAC TCGGTTGCTG CAGGAACATG GAGAAATGGA TGTTTTTGAG 
AACATGGTTG AAGCTTCCAC TGTTCAAACG AGTGCGTTTG TCCTTCGGTA CCACCACGAT 
TTTCGCTACA CGTTTAAAGA CAATGGCTAC ATCTACTTCC TGTTCTCCCG CTCTCCTGGC 
ACTAGCGACA GCACGAAGAT TACATTTATA GCACGCCTTT GCGAGAACGA CCATCATTAT 
TATTCTTACA CAGAACTTCA GCTCAACTGC ACTGTTAACA CCGAACAACA AGAGAACACA 
TTCAACAAAG TCCAAGCAGC GTATCTGGCT AAACCTGGAA AGGATTTGGC TCAGAATATC 
GTCCCGTCAA ATTTAAATGA TAAGGTTCTC TTTGGAGTGT TCAGTGCAGA TGAGGCGGAC 
GGGCGTTCAG CATTGTGCAT GTACCCGCTT AGCAGCATTA ATGCCAGGTT TGAGGAGGTG 
ATTGAGTCCT GCTACACAGG AGAAGTTCTG GAGGATGACA AACCGAAGAC TGTTTATTCA 
CCCTACAACT CCAAGAATGA AGCCATATGC AAGACCAAAA GAGATAAGAA CATGGTAAAG 
GCATATATAT GTGGCGCAGA GTTTCTGCCA TCTCCACTAG CCAGTAAACC TGAGTATGCT 
TTAGCCGTCA AACCTATCTA CACCAGGAAT GACATGCTGA CAGCTGTAGC TGTGGCGGTG 
GAGAACGAAC ACACTGTGGC ATTCTTGGGA ACCAGTGGTG CAGACGTCTT GAAGGTGCAT 
CTTGACCCAA ACCACACTGA CTTTTACAAC AGAATTCCAG GGGATCGTGC AGATGGTGCA 
GTAAACAAAA ACCTTTTGTT TGATTCAACT TTGAATCACC TGTACATCAC CACTGGCAAA 
AAGATCTCCA AAGTGTCAGT GCAGGCCTGT GAGCAGAGAG AAGATTGTCA CTCATGTGTT 
TCCCAGCGGG ACCCATATTG CGGCTGGTGT GTGTTGGAGG GCCGCTGCAC CAGGAAAAAA 
GATTGTCGAA AAGGAGATGG AGAAAACGCC TGGTTGTGGA GTCCAAAACA AACATGTGTA 
ACCATTCAGT CCTTTGAGCC CCCCAACATC AGCTGCAAGA AAACAGATAG TACACAGGTG 
GTGATCAACA TCCCGTCCCT CCCGAGCATC AGCAGTCCTG ACAGGCTGGA GTGTGTGTTT 
GACGGCTACA GGAGTCAAGC AAAGATGGAG GACACTCGGG TGACCTGCTA CCTCCCCAGC 
TCGGACTACA TCCCACCAAC ACCCGAGACA CAAGATTTTG TGGCAGTGCC AGTAAAAATC 
ACAGTGAATG ACAAGATTGA GGTGGCATCG AGTGCGTTTG AGTTTTACAA CTGTGCTGCC 
GTGGTGAAAA AATCAGAGAA TACACCATGC ATTGCATGTG TGACCAGTAA GTGGGGCTGT 
CAGTGGAATA TTCAGGATCA CACATGCAGT GATAAAGATG ACTCTGCCCA TGGGAGTCAT 
ATCATCGGAA ACATGAAGGC TAATCTGTGT CCACAGTTTG AGATTCCTGA GCCTCTGCGT 
ATTCCTGTTG GTTATAAAAC CCCCATCACA TTTCATGGCA AAAATCTGGA TATTTATTGG 
GGCAAAACCT TTCAGATCGG CACCGAGCTC ATGAAGCAGG TGGGCGAAGT GACTGTGGAG 
GCCGATGGGT TAAAGTACAG ATTTGAGGGA TATGAGTTTG CGTATGACAA AGAGCCAGAG 
GTGAATGTGA CGTTCTACAT AAAGGATAAA GACACGGATA GGAAGATTGA CAGCACCCTG 
AGAGTGGTTC TCTACAACTG TTCGGTTAGA CGCGAGGACT GCAGTCTGTG TAAAAATGCA 
CACAAGAATG ATAGCTGCGT TTGGTGCGAC ACTACAAAAT CCTGCATCTA CAGAAGCCTG 
TGTGACAATG AACTGCACCA GTGTCCTCCT CCAAAGATCA CAGACATTGT TCCCCGTTAT 
GGGCCAATGA ACGGACGCAT CTCAGTCACA ATAAAAGGCT CAAATTTGGG CATCAAGAAG 
GAGGACATTA AGAAAATCAC TGTGGCTAAA GTTCCATGCA TTCATCAGCC AGAGCGATAC 
TCGGTTTCTA CTAGTGTTGT GTGTGAGATC GGCCCTTTCC CCTTACCTCC TGAAGATCTT 
CCCATTTCAC CGAGCAAATC CGGGCCGGTG GAGGTGTTGG TGGAAGGAGA AAGAACAGGG 
ACGTCCGAGG TGGAATTCAC TTACAGGGAT CCAGTCCCAG AAGCTGTGTT CCCCGTTAAA 
GGCCCAGCAG CTGGAGGAAC TGTCATCACA ATCACAGGAC AGGACCTCGA CTCGGCCAGT 
AAAGATGACA TCACCATTAC AGTGGACAAA GAGCCATGTG AAGTGTTGAC GTTTGGCCAT 
GAGATCACCT GCAAAACTGG CCCATATAAA GGACAAAAGA TTCCCTCGGA TAAGTTGCCT 
GTTATTGTGA AGTATGGCAA GGACACTGTT AAGACAGTCG ATGCATTCGA ATACTCACCC 
GATCCCAAAA TTTCTGAATA TTTTCCACAG ACCAGCTTTG TTTGCGGTGG TCGCAGGATC 
TTAGTGATAG GCTCTGGATT TGATCAGGTT CAGAGAGCCA CCATGCGAGT GCTGCCCTCT 
TCTGACGAGT TTGACGTCAA GGCTAGCAAT GTAGAGTTCT TTCAAGAAGT GGAAAAGAAG 
AACGAGACGA CGCTGGAGTT TCTGTCGCCG GTTGTCAATA AGACATACAT CAACCTGTCC 
TTAAGAACCG TCATCCAGCT GGACAACCTG GTGCTCAATC TGACAGATTT CGCTTACCAT 
CCGGACCCAC ACTTCGATAA ACTCAAAGAT AAAGCCATAA CAGAGGGCAG CATCATCAAT 
GTTAATGGTA GAGGTTTTGC CAAAGCCATG ACTGCCAAAG AGGCACAGGC ATTTGTGGGT 
GACGAGTCGT GTCTGGTGAC AATCCTTGAG GACGATAAAC TGTTCCTGGT GGCCCCGTCC 
AGCCCTCCAA AATCCACCTC CAGACGCCAT CGAAGAGACA CCAGCTCAGA TGCCTTGGAG 
CTGCTGATCA AGTTTGGAAA TGGAGAGTGG AATGTAGGCT CCGTGCGCTT CGAGAGGAAA 
TACGAGGTTC CCCTTTACAT CATAATCCCC GCTGTTCTCA TTCCCATGGT GCTCTTCATT 
GCGGTGTCCA TCTATTGCTA CAGGAGGAAA AGCCAGCAGG CAGAACGAGA ATATGAGAAG 
ATCAAAAATC AGCTGGCCAA TTTGGAGGAG AGCGTCCGTG ATCGCTGTAA AAAGGAGTTC 
ACAGATCTAA TGATAGAGAT GGAGGATCAC ACCAGTGATC TGAGTGAAGC TCGCATCCCA 
TTCCTGGACT ACAAGAACTA CACCGACCGT GTTTTCTTCC TGCCCTCGAA AGATGGAGCC 
AATGATGTCA TGATCACCGG CAAGCTGGAT ATCCCTGAAA ACCGCAGAGC CATCGTAGAT 
CAGGCACTCA ACCAGTTCTC CAACCTCCTG AACAGCAAAA CCTTCCTTAT CAATTTTATT 
CGTACCCTGG AGGGCCGACA GGAGTTCAAT GCCAGGAATA AGGTTTACTT CGCCTCTTTG 
TTGACGGTGG CACTGCATGG CAAGTTGGAG TATTACACGG ACATCATGAG GACTCTCCTG 
CTGGAGCTCA TGGAGGAATA TGTTCACAGC AAGAATCCCA AACTCATGCT CCGCAGATCC 
GAGACTGTTG TGGAGAGGAT GTTGTGCAAC TGGATGTCGA TTTGTCTCTA CCAGTTCCTG 
AAGGACTCAG CCGGCGAGCC GCTTTATAAG CTCTTTAAGG CCATTAAACA CCAGGTGGAA 
AAAGGCCCTG TTGATGCCAA GATGAAGAAA GCCAAGTACA CTCTCAACGA CACGGGGCTT 
CTGGGAGATG ATGTTGAGTA CAGCATTTTG ACCCTACAGG TGCTGGTTCA TGGCGAGGGT 
CCGGACGTGA CCCCGGTCAA AGTGCTAAAC TGCGACACCA TCTCGCAGGT GAAGGAGAAG 
ATCATCGAGC AGGTGTACAG GAATCTGCCA TATTCCCAGA GGCCCGTGGT GGACAGCGTC 
GCACTGGAGT GGCGTCCTGG TTCTACAGGG CAGATTCTGT CTGATCTGGA CCTGACCTCA 
CAGAAGGAGG GAAGGTGGAA ACGGATCAAC ACACTGGCAC ACTATAATGT GCGAGACGGA 
GCCACCCTGG TCTTATCCAG AGTCCTGCAC ACTCAGCAGT CCTTCGACCA GAACATGGAC 
AACCATGAAG AAAGAAATGC TTTGCTGGAG GATGATAAAG TGTTTCACTT GGTGAGACCG 
GCGGATGAGC TGGAGGAGGT CAAATCTAAG AGAGGGAGCA TGAAGGACAA GGCCACCACA 
AAAGCCATCA CTGAGATCTA CCTGACCAGA CTGCTCTCCA TGAAGGGAAC GCTGCAGCAG 
TTTGTAGATG ATTTCTTCCG CAGTGTGTTG TGTTCAGGGA CTGTGGTTCC TCCAGCCGTC 
AAGTACTTCT TTGACTTCTT AGATGAACAA GCTCAAAAAC ATGACAACGT TGATGATGAG 
ACCATCCACA TATGGAAGAC CAACAGTCTT CCTCTCCGTT TCTGGGTGAA CATCTTGAAG 
AATCCTCACT TCATCTTCGA TGTTCACCTG ACTGAGGTAG TGGACGCATC TCTGTCCGTC 
ATCGCTCAGA CCTTCATGGA CGCCTGCACT AAAACCGAGC ACAAACTGAC GCGGGAGTCT 
CCCAGTAACA AACTGCTTTA TGCAAAGGAA ATCTCCACGT ACAAGAAGAT GGTTGATGAT 
TATTATAAAG GCATTCGGCA GATGGTTCCA GTCAGTGACC AAGACATGAA CACACACCTC 
GCAGAGATTT CACGGTCTCA CACAGATAAG TTGAACACCC AGGCCGCGCT GCACCAGCTC 
TACCAGTATG CCAGCAAGTA CTATGACGGG ATCATCTCGT CACTGGAGGA AGATCCTGCC 
GCCCAGAGCA AACAACTGAC CCTCAGACTG CAACAGATCG CCGCTGCGCT GGAGAATAAA 
GTCACCGACC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298559.1
CDS	525..6119
Translation	MAWRALAAFSLLLCFGSSCCDDAQEQFSSPTIINNVVQDPQTGRIYLGAVNGIFQLNHNLQEESRADTGPKKDNPQCTPPITAQCTDAKEMDNFNKLLLVNSANGTLIVCGSLFRGICSLVNLNSVNKPVYYSDTKGEKTYVASTEESVTVVGVISYFKDTTTNANLSVFLVGKGYGSSDSSKLISTRLLQEHGEMDVFENMVEASTVQTSAFVLRYHHDFRYTFKDNGYIYFLFSRSPGTSDSTKITFIARLCENDHHYYSYTELQLNCTVNTEQQENTFNKVQAAYLAKPGKDLAQNIVPSNLNDKVLFGVFSADEADGRSALCMYPLSSINARFEEVIESCYTGEVLEDDKPKTVYSPYNSKNEAICKTKRDKNMVKAYICGAEFLPSPLASKPEYALAVKPIYTRNDMLTAVAVAVENEHTVAFLGTSGADVLKVHLDPNHTDFYNRIPGDRADGAVNKNLLFDSTLNHLYITTGKKISKVSVQACEQREDCHSCVSQRDPYCGWCVLEGRCTRKKDCRKGDGENAWLWSPKQTCVTIQSFEPPNISCKKTDSTQVVINIPSLPSISSPDRLECVFDGYRSQAKMEDTRVTCYLPSSDYIPPTPETQDFVAVPVKITVNDKIEVASSAFEFYNCAAVVKKSENTPCIACVTSKWGCQWNIQDHTCSDKDDSAHGSHIIGNMKANLCPQFEIPEPLRIPVGYKTPITFHGKNLDIYWGKTFQIGTELMKQVGEVTVEADGLKYRFEGYEFAYDKEPEVNVTFYIKDKDTDRKIDSTLRVVLYNCSVRREDCSLCKNAHKNDSCVWCDTTKSCIYRSLCDNELHQCPPPKITDIVPRYGPMNGRISVTIKGSNLGIKKEDIKKITVAKVPCIHQPERYSVSTSVVCEIGPFPLPPEDLPISPSKSGPVEVLVEGERTGTSEVEFTYRDPVPEAVFPVKGPAAGGTVITITGQDLDSASKDDITITVDKEPCEVLTFGHEITCKTGPYKGQKIPSDKLPVIVKYGKDTVKTVDAFEYSPDPKISEYFPQTSFVCGGRRILVIGSGFDQVQRATMRVLPSSDEFDVKASNVEFFQEVEKKNETTLEFLSPVVNKTYINLSLRTVIQLDNLVLNLTDFAYHPDPHFDKLKDKAITEGSIINVNGRGFAKAMTAKEAQAFVGDESCLVTILEDDKLFLVAPSSPPKSTSRRHRRDTSSDALELLIKFGNGEWNVGSVRFERKYEVPLYIIIPAVLIPMVLFIAVSIYCYRRKSQQAEREYEKIKNQLANLEESVRDRCKKEFTDLMIEMEDHTSDLSEARIPFLDYKNYTDRVFFLPSKDGANDVMITGKLDIPENRRAIVDQALNQFSNLLNSKTFLINFIRTLEGRQEFNARNKVYFASLLTVALHGKLEYYTDIMRTLLLELMEEYVHSKNPKLMLRRSETVVERMLCNWMSICLYQFLKDSAGEPLYKLFKAIKHQVEKGPVDAKMKKAKYTLNDTGLLGDDVEYSILTLQVLVHGEGPDVTPVKVLNCDTISQVKEKIIEQVYRNLPYSQRPVVDSVALEWRPGSTGQILSDLDLTSQKEGRWKRINTLAHYNVRDGATLVLSRVLHTQQSFDQNMDNHEERNALLEDDKVFHLVRPADELEEVKSKRGSMKDKATTKAITEIYLTRLLSMKGTLQQFVDDFFRSVLCSGTVVPPAVKYFFDFLDEQAQKHDNVDDETIHIWKTNSLPLRFWVNILKNPHFIFDVHLTEVVDASLSVIAQTFMDACTKTEHKLTRESPSNKLLYAKEISTYKKMVDDYYKGIRQMVPVSDQDMNTHLAEISRSHTDKLNTQAALHQLYQYASKYYDGIISSLEEDPAAQSKQLTLRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	XM_009300284.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio plexin b2a (plxnb2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300284.3

ORF Insert Sequence:

ATGGCGTGGA GGGCCCTGGC AGCTTTCTCT CTACTACTGT GTTTTGGCTC ATCTTGCTGT 
GATGATGCTC AAGAACAGTT CTCGTCTCCT ACCATCATCA ATAATGTGGT CCAGGACCCT 
CAAACAGGCC GAATCTACCT TGGAGCTGTC AATGGGATCT TTCAGCTGAA CCACAATCTA 
CAGGAGGAGA GCAGAGCAGA CACAGGCCCT AAGAAAGACA ACCCACAATG CACTCCACCT 
ATCACTGCGC AGTGCACCGA TGCGAAGGAA ATGGACAACT TTAACAAACT CCTTCTGGTT 
AACTCCGCCA ACGGCACCTT GATCGTTTGT GGAAGCCTTT TCAGGGGCAT CTGCTCTTTG 
GTAAACCTCA ACAGTGTGAA TAAACCGGTG TACTACAGTG ACACCAAAGG AGAGAAGACA 
TATGTTGCCA GCACTGAGGA ATCTGTCACT GTGGTGGGGG TCATTTCTTA TTTTAAGGAC 
ACTACTACTA ATGCTAACCT CAGTGTATTT CTAGTGGGAA AAGGCTATGG AAGTTCGGAC 
AGCTCAAAGC TCATTAGCAC TCGGTTGCTG CAGGAACATG GAGAAATGGA TGTTTTTGAG 
AACATGGTTG AAGCTTCCAC TGTTCAAACG AGTGCGTTTG TCCTTCGGTA CCACCACGAT 
TTTCGCTACA CGTTTAAAGA CAATGGCTAC ATCTACTTCC TGTTCTCCCG CTCTCCTGGC 
ACTAGCGACA GCACGAAGAT TACATTTATA GCACGCCTTT GCGAGAACGA CCATCATTAT 
TATTCTTACA CAGAACTTCA GCTCAACTGC ACTGTTAACA CCGAACAACA AGAGAACACA 
TTCAACAAAG TCCAAGCAGC GTATCTGGCT AAACCTGGAA AGGATTTGGC TCAGAATATC 
GTCCCGTCAA ATTTAAATGA TAAGGTTCTC TTTGGAGTGT TCAGTGCAGA TGAGGCGGAC 
GGGCGTTCAG CATTGTGCAT GTACCCGCTT AGCAGCATTA ATGCCAGGTT TGAGGAGGTG 
ATTGAGTCCT GCTACACAGG AGAAGTTCTG GAGGATGACA AACCGAAGAC TGTTTATTCA 
CCCTACAACT CCAAGAATGA AGCCATATGC AAGACCAAAA GAGATAAGAA CATGGTAAAG 
GCATATATAT GTGGCGCAGA GTTTCTGCCA TCTCCACTAG CCAGTAAACC TGAGTATGCT 
TTAGCCGTCA AACCTATCTA CACCAGGAAT GACATGCTGA CAGCTGTAGC TGTGGCGGTG 
GAGAACGAAC ACACTGTGGC ATTCTTGGGA ACCAGTGGTG CAGACGTCTT GAAGGTGCAT 
CTTGACCCAA ACCACACTGA CTTTTACAAC AGAATTCCAG GGGATCGTGC AGATGGTGCA 
GTAAACAAAA ACCTTTTGTT TGATTCAACT TTGAATCACC TGTACATCAC CACTGGCAAA 
AAGATCTCCA AAGTGTCAGT GCAGGCCTGT GAGCAGAGAG AAGATTGTCA CTCATGTGTT 
TCCCAGCGGG ACCCATATTG CGGCTGGTGT GTGTTGGAGG GCCGCTGCAC CAGGAAAAAA 
GATTGTCGAA AAGGAGATGG AGAAAACGCC TGGTTGTGGA GTCCAAAACA AACATGTGTA 
ACCATTCAGT CCTTTGAGCC CCCCAACATC AGCTGCAAGA AAACAGATAG TACACAGGTG 
GTGATCAACA TCCCGTCCCT CCCGAGCATC AGCAGTCCTG ACAGGCTGGA GTGTGTGTTT 
GACGGCTACA GGAGTCAAGC AAAGATGGAG GACACTCGGG TGACCTGCTA CCTCCCCAGC 
TCGGACTACA TCCCACCAAC ACCCGAGACA CAAGATTTTG TGGCAGTGCC AGTAAAAATC 
ACAGTGAATG ACAAGATTGA GGTGGCATCG AGTGCGTTTG AGTTTTACAA CTGTGCTGCC 
GTGGTGAAAA AATCAGAGAA TACACCATGC ATTGCATGTG TGACCAGTAA GTGGGGCTGT 
CAGTGGAATA TTCAGGATCA CACATGCAGT GATAAAGATG ACTCTGCCCA TGGGAGTCAT 
ATCATCGGAA ACATGAAGGC TAATCTGTGT CCACAGTTTG AGATTCCTGA GCCTCTGCGT 
ATTCCTGTTG GTTATAAAAC CCCCATCACA TTTCATGGCA AAAATCTGGA TATTTATTGG 
GGCAAAACCT TTCAGATCGG CACCGAGCTC ATGAAGCAGG TGGGCGAAGT GACTGTGGAG 
GCCGATGGGT TAAAGTACAG ATTTGAGGGA TATGAGTTTG CGTATGACAA AGAGCCAGAG 
GTGAATGTGA CGTTCTACAT AAAGGATAAA GACACGGATA GGAAGATTGA CAGCACCCTG 
AGAGTGGTTC TCTACAACTG TTCGGTTAGA CGCGAGGACT GCAGTCTGTG TAAAAATGCA 
CACAAGAATG ATAGCTGCGT TTGGTGCGAC ACTACAAAAT CCTGCATCTA CAGAAGCCTG 
TGTGACAATG AACTGCACCA GTGTCCTCCT CCAAAGATCA CAGACATTGT TCCCCGTTAT 
GGGCCAATGA ACGGACGCAT CTCAGTCACA ATAAAAGGCT CAAATTTGGG CATCAAGAAG 
GAGGACATTA AGAAAATCAC TGTGGCTAAA GTTCCATGCA TTCATCAGCC AGAGCGATAC 
TCGGTTTCTA CTAGTGTTGT GTGTGAGATC GGCCCTTTCC CCTTACCTCC TGAAGATCTT 
CCCATTTCAC CGAGCAAATC CGGGCCGGTG GAGGTGTTGG TGGAAGGAGA AAGAACAGGG 
ACGTCCGAGG TGGAATTCAC TTACAGGGAT CCAGTCCCAG AAGCTGTGTT CCCCGTTAAA 
GGCCCAGCAG CTGGAGGAAC TGTCATCACA ATCACAGGAC AGGACCTCGA CTCGGCCAGT 
AAAGATGACA TCACCATTAC AGTGGACAAA GAGCCATGTG AAGTGTTGAC GTTTGGCCAT 
GAGATCACCT GCAAAACTGG CCCATATAAA GGACAAAAGA TTCCCTCGGA TAAGTTGCCT 
GTTATTGTGA AGTATGGCAA GGACACTGTT AAGACAGTCG ATGCATTCGA ATACTCACCC 
GATCCCAAAA TTTCTGAATA TTTTCCACAG ACCAGCTTTG TTTGCGGTGG TCGCAGGATC 
TTAGTGATAG GCTCTGGATT TGATCAGGTT CAGAGAGCCA CCATGCGAGT GCTGCCCTCT 
TCTGACGAGT TTGACGTCAA GGCTAGCAAT GTAGAGTTCT TTCAAGAAGT GGAAAAGAAG 
AACGAGACGA CGCTGGAGTT TCTGTCGCCG GTTGTCAATA AGACATACAT CAACCTGTCC 
TTAAGAACCG TCATCCAGCT GGACAACCTG GTGCTCAATC TGACAGATTT CGCTTACCAT 
CCGGACCCAC ACTTCGATAA ACTCAAAGAT AAAGCCATAA CAGAGGGCAG CATCATCAAT 
GTTAATGGTA GAGGTTTTGC CAAAGCCATG ACTGCCAAAG AGGCACAGGC ATTTGTGGGT 
GACGAGTCGT GTCTGGTGAC AATCCTTGAG GACGATAAAC TGTTCCTGGT GGCCCCGTCC 
AGCCCTCCAA AATCCACCTC CAGACGCCAT CGAAGAGACA CCAGCTCAGA TGCCTTGGAG 
CTGCTGATCA AGTTTGGAAA TGGAGAGTGG AATGTAGGCT CCGTGCGCTT CGAGAGGAAA 
TACGAGGTTC CCCTTTACAT CATAATCCCC GCTGTTCTCA TTCCCATGGT GCTCTTCATT 
GCGGTGTCCA TCTATTGCTA CAGGAGGAAA AGCCAGCAGG CAGAACGAGA ATATGAGAAG 
ATCAAAAATC AGCTGGCCAA TTTGGAGGAG AGCGTCCGTG ATCGCTGTAA AAAGGAGTTC 
ACAGATCTAA TGATAGAGAT GGAGGATCAC ACCAGTGATC TGAGTGAAGC TCGCATCCCA 
TTCCTGGACT ACAAGAACTA CACCGACCGT GTTTTCTTCC TGCCCTCGAA AGATGGAGCC 
AATGATGTCA TGATCACCGG CAAGCTGGAT ATCCCTGAAA ACCGCAGAGC CATCGTAGAT 
CAGGCACTCA ACCAGTTCTC CAACCTCCTG AACAGCAAAA CCTTCCTTAT CAATTTTATT 
CGTACCCTGG AGGGCCGACA GGAGTTCAAT GCCAGGAATA AGGTTTACTT CGCCTCTTTG 
TTGACGGTGG CACTGCATGG CAAGTTGGAG TATTACACGG ACATCATGAG GACTCTCCTG 
CTGGAGCTCA TGGAGGAATA TGTTCACAGC AAGAATCCCA AACTCATGCT CCGCAGATCC 
GAGACTGTTG TGGAGAGGAT GTTGTGCAAC TGGATGTCGA TTTGTCTCTA CCAGTTCCTG 
AAGGACTCAG CCGGCGAGCC GCTTTATAAG CTCTTTAAGG CCATTAAACA CCAGGTGGAA 
AAAGGCCCTG TTGATGCCAA GATGAAGAAA GCCAAGTACA CTCTCAACGA CACGGGGCTT 
CTGGGAGATG ATGTTGAGTA CAGCATTTTG ACCCTACAGG TGCTGGTTCA TGGCGAGGGT 
CCGGACGTGA CCCCGGTCAA AGTGCTAAAC TGCGACACCA TCTCGCAGGT GAAGGAGAAG 
ATCATCGAGC AGGTGTACAG GAATCTGCCA TATTCCCAGA GGCCCGTGGT GGACAGCGTC 
GCACTGGAGT GGCGTCCTGG TTCTACAGGG CAGATTCTGT CTGATCTGGA CCTGACCTCA 
CAGAAGGAGG GAAGGTGGAA ACGGATCAAC ACACTGGCAC ACTATAATGT GCGAGACGGA 
GCCACCCTGG TCTTATCCAG AGTCCTGCAC ACTCAGCAGT CCTTCGACCA GAACATGGAC 
AACCATGAAG AAAGAAATGC TTTGCTGGAG GATGATAAAG TGTTTCACTT GGTGAGACCG 
GCGGATGAGC TGGAGGAGGT CAAATCTAAG AGAGGGAGCA TGAAGGACAA GGCCACCACA 
AAAGCCATCA CTGAGATCTA CCTGACCAGA CTGCTCTCCA TGAAGGGAAC GCTGCAGCAG 
TTTGTAGATG ATTTCTTCCG CAGTGTGTTG TGTTCAGGGA CTGTGGTTCC TCCAGCCGTC 
AAGTACTTCT TTGACTTCTT AGATGAACAA GCTCAAAAAC ATGACAACGT TGATGATGAG 
ACCATCCACA TATGGAAGAC CAACAGTCTT CCTCTCCGTT TCTGGGTGAA CATCTTGAAG 
AATCCTCACT TCATCTTCGA TGTTCACCTG ACTGAGGTAG TGGACGCATC TCTGTCCGTC 
ATCGCTCAGA CCTTCATGGA CGCCTGCACT AAAACCGAGC ACAAACTGAC GCGGGAGTCT 
CCCAGTAACA AACTGCTTTA TGCAAAGGAA ATCTCCACGT ACAAGAAGAT GGTTGATGAT 
TATTATAAAG GCATTCGGCA GATGGTTCCA GTCAGTGACC AAGACATGAA CACACACCTC 
GCAGAGATTT CACGGTCTCA CACAGATAAG TTGAACACCC AGGCCGCGCT GCACCAGCTC 
TACCAGTATG CCAGCAAGTA CTATGACGGG ATCATCTCGT CACTGGAGGA AGATCCTGCC 
GCCCAGAGCA AACAACTGAC CCTCAGACTG CAACAGATCG CCGCTGCGCT GGAGAATAAA 
GTCACCGACC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLXNB2A ( XM_009300283.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298558.1 Danio rerio plexin b2a (plxnb2a), transcript variant X2, mRNA.

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CloneID	ODa43506
Clone ID Related Accession (Same CDS sequence)	XM_009300284.3 , XM_009300283.2 , XM_009300283.3 , XM_009300284.2
Accession Version	XM_009300283.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plexin-B2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTGGA GGGCCCTGGC AGCTTTCTCT CTACTACTGT GTTTTGGCTC ATCTTGCTGT 
GATGATGCTC AAGAACAGTT CTCGTCTCCT ACCATCATCA ATAATGTGGT CCAGGACCCT 
CAAACAGGCC GAATCTACCT TGGAGCTGTC AATGGGATCT TTCAGCTGAA CCACAATCTA 
CAGGAGGAGA GCAGAGCAGA CACAGGCCCT AAGAAAGACA ACCCACAATG CACTCCACCT 
ATCACTGCGC AGTGCACCGA TGCGAAGGAA ATGGACAACT TTAACAAACT CCTTCTGGTT 
AACTCCGCCA ACGGCACCTT GATCGTTTGT GGAAGCCTTT TCAGGGGCAT CTGCTCTTTG 
GTAAACCTCA ACAGTGTGAA TAAACCGGTG TACTACAGTG ACACCAAAGG AGAGAAGACA 
TATGTTGCCA GCACTGAGGA ATCTGTCACT GTGGTGGGGG TCATTTCTTA TTTTAAGGAC 
ACTACTACTA ATGCTAACCT CAGTGTATTT CTAGTGGGAA AAGGCTATGG AAGTTCGGAC 
AGCTCAAAGC TCATTAGCAC TCGGTTGCTG CAGGAACATG GAGAAATGGA TGTTTTTGAG 
AACATGGTTG AAGCTTCCAC TGTTCAAACG AGTGCGTTTG TCCTTCGGTA CCACCACGAT 
TTTCGCTACA CGTTTAAAGA CAATGGCTAC ATCTACTTCC TGTTCTCCCG CTCTCCTGGC 
ACTAGCGACA GCACGAAGAT TACATTTATA GCACGCCTTT GCGAGAACGA CCATCATTAT 
TATTCTTACA CAGAACTTCA GCTCAACTGC ACTGTTAACA CCGAACAACA AGAGAACACA 
TTCAACAAAG TCCAAGCAGC GTATCTGGCT AAACCTGGAA AGGATTTGGC TCAGAATATC 
GTCCCGTCAA ATTTAAATGA TAAGGTTCTC TTTGGAGTGT TCAGTGCAGA TGAGGCGGAC 
GGGCGTTCAG CATTGTGCAT GTACCCGCTT AGCAGCATTA ATGCCAGGTT TGAGGAGGTG 
ATTGAGTCCT GCTACACAGG AGAAGTTCTG GAGGATGACA AACCGAAGAC TGTTTATTCA 
CCCTACAACT CCAAGAATGA AGCCATATGC AAGACCAAAA GAGATAAGAA CATGGTAAAG 
GCATATATAT GTGGCGCAGA GTTTCTGCCA TCTCCACTAG CCAGTAAACC TGAGTATGCT 
TTAGCCGTCA AACCTATCTA CACCAGGAAT GACATGCTGA CAGCTGTAGC TGTGGCGGTG 
GAGAACGAAC ACACTGTGGC ATTCTTGGGA ACCAGTGGTG CAGACGTCTT GAAGGTGCAT 
CTTGACCCAA ACCACACTGA CTTTTACAAC AGAATTCCAG GGGATCGTGC AGATGGTGCA 
GTAAACAAAA ACCTTTTGTT TGATTCAACT TTGAATCACC TGTACATCAC CACTGGCAAA 
AAGATCTCCA AAGTGTCAGT GCAGGCCTGT GAGCAGAGAG AAGATTGTCA CTCATGTGTT 
TCCCAGCGGG ACCCATATTG CGGCTGGTGT GTGTTGGAGG GCCGCTGCAC CAGGAAAAAA 
GATTGTCGAA AAGGAGATGG AGAAAACGCC TGGTTGTGGA GTCCAAAACA AACATGTGTA 
ACCATTCAGT CCTTTGAGCC CCCCAACATC AGCTGCAAGA AAACAGATAG TACACAGGTG 
GTGATCAACA TCCCGTCCCT CCCGAGCATC AGCAGTCCTG ACAGGCTGGA GTGTGTGTTT 
GACGGCTACA GGAGTCAAGC AAAGATGGAG GACACTCGGG TGACCTGCTA CCTCCCCAGC 
TCGGACTACA TCCCACCAAC ACCCGAGACA CAAGATTTTG TGGCAGTGCC AGTAAAAATC 
ACAGTGAATG ACAAGATTGA GGTGGCATCG AGTGCGTTTG AGTTTTACAA CTGTGCTGCC 
GTGGTGAAAA AATCAGAGAA TACACCATGC ATTGCATGTG TGACCAGTAA GTGGGGCTGT 
CAGTGGAATA TTCAGGATCA CACATGCAGT GATAAAGATG ACTCTGCCCA TGGGAGTCAT 
ATCATCGGAA ACATGAAGGC TAATCTGTGT CCACAGTTTG AGATTCCTGA GCCTCTGCGT 
ATTCCTGTTG GTTATAAAAC CCCCATCACA TTTCATGGCA AAAATCTGGA TATTTATTGG 
GGCAAAACCT TTCAGATCGG CACCGAGCTC ATGAAGCAGG TGGGCGAAGT GACTGTGGAG 
GCCGATGGGT TAAAGTACAG ATTTGAGGGA TATGAGTTTG CGTATGACAA AGAGCCAGAG 
GTGAATGTGA CGTTCTACAT AAAGGATAAA GACACGGATA GGAAGATTGA CAGCACCCTG 
AGAGTGGTTC TCTACAACTG TTCGGTTAGA CGCGAGGACT GCAGTCTGTG TAAAAATGCA 
CACAAGAATG ATAGCTGCGT TTGGTGCGAC ACTACAAAAT CCTGCATCTA CAGAAGCCTG 
TGTGACAATG AACTGCACCA GTGTCCTCCT CCAAAGATCA CAGACATTGT TCCCCGTTAT 
GGGCCAATGA ACGGACGCAT CTCAGTCACA ATAAAAGGCT CAAATTTGGG CATCAAGAAG 
GAGGACATTA AGAAAATCAC TGTGGCTAAA GTTCCATGCA TTCATCAGCC AGAGCGATAC 
TCGGTTTCTA CTAGTGTTGT GTGTGAGATC GGCCCTTTCC CCTTACCTCC TGAAGATCTT 
CCCATTTCAC CGAGCAAATC CGGGCCGGTG GAGGTGTTGG TGGAAGGAGA AAGAACAGGG 
ACGTCCGAGG TGGAATTCAC TTACAGGGAT CCAGTCCCAG AAGCTGTGTT CCCCGTTAAA 
GGCCCAGCAG CTGGAGGAAC TGTCATCACA ATCACAGGAC AGGACCTCGA CTCGGCCAGT 
AAAGATGACA TCACCATTAC AGTGGACAAA GAGCCATGTG AAGTGTTGAC GTTTGGCCAT 
GAGATCACCT GCAAAACTGG CCCATATAAA GGACAAAAGA TTCCCTCGGA TAAGTTGCCT 
GTTATTGTGA AGTATGGCAA GGACACTGTT AAGACAGTCG ATGCATTCGA ATACTCACCC 
GATCCCAAAA TTTCTGAATA TTTTCCACAG ACCAGCTTTG TTTGCGGTGG TCGCAGGATC 
TTAGTGATAG GCTCTGGATT TGATCAGGTT CAGAGAGCCA CCATGCGAGT GCTGCCCTCT 
TCTGACGAGT TTGACGTCAA GGCTAGCAAT GTAGAGTTCT TTCAAGAAGT GGAAAAGAAG 
AACGAGACGA CGCTGGAGTT TCTGTCGCCG GTTGTCAATA AGACATACAT CAACCTGTCC 
TTAAGAACCG TCATCCAGCT GGACAACCTG GTGCTCAATC TGACAGATTT CGCTTACCAT 
CCGGACCCAC ACTTCGATAA ACTCAAAGAT AAAGCCATAA CAGAGGGCAG CATCATCAAT 
GTTAATGGTA GAGGTTTTGC CAAAGCCATG ACTGCCAAAG AGGCACAGGC ATTTGTGGGT 
GACGAGTCGT GTCTGGTGAC AATCCTTGAG GACGATAAAC TGTTCCTGGT GGCCCCGTCC 
AGCCCTCCAA AATCCACCTC CAGACGCCAT CGAAGAGACA CCAGCTCAGA TGCCTTGGAG 
CTGCTGATCA AGTTTGGAAA TGGAGAGTGG AATGTAGGCT CCGTGCGCTT CGAGAGGAAA 
TACGAGGTTC CCCTTTACAT CATAATCCCC GCTGTTCTCA TTCCCATGGT GCTCTTCATT 
GCGGTGTCCA TCTATTGCTA CAGGAGGAAA AGCCAGCAGG CAGAACGAGA ATATGAGAAG 
ATCAAAAATC AGCTGGCCAA TTTGGAGGAG AGCGTCCGTG ATCGCTGTAA AAAGGAGTTC 
ACAGATCTAA TGATAGAGAT GGAGGATCAC ACCAGTGATC TGAGTGAAGC TCGCATCCCA 
TTCCTGGACT ACAAGAACTA CACCGACCGT GTTTTCTTCC TGCCCTCGAA AGATGGAGCC 
AATGATGTCA TGATCACCGG CAAGCTGGAT ATCCCTGAAA ACCGCAGAGC CATCGTAGAT 
CAGGCACTCA ACCAGTTCTC CAACCTCCTG AACAGCAAAA CCTTCCTTAT CAATTTTATT 
CGTACCCTGG AGGGCCGACA GGAGTTCAAT GCCAGGAATA AGGTTTACTT CGCCTCTTTG 
TTGACGGTGG CACTGCATGG CAAGTTGGAG TATTACACGG ACATCATGAG GACTCTCCTG 
CTGGAGCTCA TGGAGGAATA TGTTCACAGC AAGAATCCCA AACTCATGCT CCGCAGATCC 
GAGACTGTTG TGGAGAGGAT GTTGTGCAAC TGGATGTCGA TTTGTCTCTA CCAGTTCCTG 
AAGGACTCAG CCGGCGAGCC GCTTTATAAG CTCTTTAAGG CCATTAAACA CCAGGTGGAA 
AAAGGCCCTG TTGATGCCAA GATGAAGAAA GCCAAGTACA CTCTCAACGA CACGGGGCTT 
CTGGGAGATG ATGTTGAGTA CAGCATTTTG ACCCTACAGG TGCTGGTTCA TGGCGAGGGT 
CCGGACGTGA CCCCGGTCAA AGTGCTAAAC TGCGACACCA TCTCGCAGGT GAAGGAGAAG 
ATCATCGAGC AGGTGTACAG GAATCTGCCA TATTCCCAGA GGCCCGTGGT GGACAGCGTC 
GCACTGGAGT GGCGTCCTGG TTCTACAGGG CAGATTCTGT CTGATCTGGA CCTGACCTCA 
CAGAAGGAGG GAAGGTGGAA ACGGATCAAC ACACTGGCAC ACTATAATGT GCGAGACGGA 
GCCACCCTGG TCTTATCCAG AGTCCTGCAC ACTCAGCAGT CCTTCGACCA GAACATGGAC 
AACCATGAAG AAAGAAATGC TTTGCTGGAG GATGATAAAG TGTTTCACTT GGTGAGACCG 
GCGGATGAGC TGGAGGAGGT CAAATCTAAG AGAGGGAGCA TGAAGGACAA GGCCACCACA 
AAAGCCATCA CTGAGATCTA CCTGACCAGA CTGCTCTCCA TGAAGGGAAC GCTGCAGCAG 
TTTGTAGATG ATTTCTTCCG CAGTGTGTTG TGTTCAGGGA CTGTGGTTCC TCCAGCCGTC 
AAGTACTTCT TTGACTTCTT AGATGAACAA GCTCAAAAAC ATGACAACGT TGATGATGAG 
ACCATCCACA TATGGAAGAC CAACAGTCTT CCTCTCCGTT TCTGGGTGAA CATCTTGAAG 
AATCCTCACT TCATCTTCGA TGTTCACCTG ACTGAGGTAG TGGACGCATC TCTGTCCGTC 
ATCGCTCAGA CCTTCATGGA CGCCTGCACT AAAACCGAGC ACAAACTGAC GCGGGAGTCT 
CCCAGTAACA AACTGCTTTA TGCAAAGGAA ATCTCCACGT ACAAGAAGAT GGTTGATGAT 
TATTATAAAG GCATTCGGCA GATGGTTCCA GTCAGTGACC AAGACATGAA CACACACCTC 
GCAGAGATTT CACGGTCTCA CACAGATAAG TTGAACACCC AGGCCGCGCT GCACCAGCTC 
TACCAGTATG CCAGCAAGTA CTATGACGGG ATCATCTCGT CACTGGAGGA AGATCCTGCC 
GCCCAGAGCA AACAACTGAC CCTCAGACTG CAACAGATCG CCGCTGCGCT GGAGAATAAA 
GTCACCGACC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298558.1
CDS	680..6274
Translation	MAWRALAAFSLLLCFGSSCCDDAQEQFSSPTIINNVVQDPQTGRIYLGAVNGIFQLNHNLQEESRADTGPKKDNPQCTPPITAQCTDAKEMDNFNKLLLVNSANGTLIVCGSLFRGICSLVNLNSVNKPVYYSDTKGEKTYVASTEESVTVVGVISYFKDTTTNANLSVFLVGKGYGSSDSSKLISTRLLQEHGEMDVFENMVEASTVQTSAFVLRYHHDFRYTFKDNGYIYFLFSRSPGTSDSTKITFIARLCENDHHYYSYTELQLNCTVNTEQQENTFNKVQAAYLAKPGKDLAQNIVPSNLNDKVLFGVFSADEADGRSALCMYPLSSINARFEEVIESCYTGEVLEDDKPKTVYSPYNSKNEAICKTKRDKNMVKAYICGAEFLPSPLASKPEYALAVKPIYTRNDMLTAVAVAVENEHTVAFLGTSGADVLKVHLDPNHTDFYNRIPGDRADGAVNKNLLFDSTLNHLYITTGKKISKVSVQACEQREDCHSCVSQRDPYCGWCVLEGRCTRKKDCRKGDGENAWLWSPKQTCVTIQSFEPPNISCKKTDSTQVVINIPSLPSISSPDRLECVFDGYRSQAKMEDTRVTCYLPSSDYIPPTPETQDFVAVPVKITVNDKIEVASSAFEFYNCAAVVKKSENTPCIACVTSKWGCQWNIQDHTCSDKDDSAHGSHIIGNMKANLCPQFEIPEPLRIPVGYKTPITFHGKNLDIYWGKTFQIGTELMKQVGEVTVEADGLKYRFEGYEFAYDKEPEVNVTFYIKDKDTDRKIDSTLRVVLYNCSVRREDCSLCKNAHKNDSCVWCDTTKSCIYRSLCDNELHQCPPPKITDIVPRYGPMNGRISVTIKGSNLGIKKEDIKKITVAKVPCIHQPERYSVSTSVVCEIGPFPLPPEDLPISPSKSGPVEVLVEGERTGTSEVEFTYRDPVPEAVFPVKGPAAGGTVITITGQDLDSASKDDITITVDKEPCEVLTFGHEITCKTGPYKGQKIPSDKLPVIVKYGKDTVKTVDAFEYSPDPKISEYFPQTSFVCGGRRILVIGSGFDQVQRATMRVLPSSDEFDVKASNVEFFQEVEKKNETTLEFLSPVVNKTYINLSLRTVIQLDNLVLNLTDFAYHPDPHFDKLKDKAITEGSIINVNGRGFAKAMTAKEAQAFVGDESCLVTILEDDKLFLVAPSSPPKSTSRRHRRDTSSDALELLIKFGNGEWNVGSVRFERKYEVPLYIIIPAVLIPMVLFIAVSIYCYRRKSQQAEREYEKIKNQLANLEESVRDRCKKEFTDLMIEMEDHTSDLSEARIPFLDYKNYTDRVFFLPSKDGANDVMITGKLDIPENRRAIVDQALNQFSNLLNSKTFLINFIRTLEGRQEFNARNKVYFASLLTVALHGKLEYYTDIMRTLLLELMEEYVHSKNPKLMLRRSETVVERMLCNWMSICLYQFLKDSAGEPLYKLFKAIKHQVEKGPVDAKMKKAKYTLNDTGLLGDDVEYSILTLQVLVHGEGPDVTPVKVLNCDTISQVKEKIIEQVYRNLPYSQRPVVDSVALEWRPGSTGQILSDLDLTSQKEGRWKRINTLAHYNVRDGATLVLSRVLHTQQSFDQNMDNHEERNALLEDDKVFHLVRPADELEEVKSKRGSMKDKATTKAITEIYLTRLLSMKGTLQQFVDDFFRSVLCSGTVVPPAVKYFFDFLDEQAQKHDNVDDETIHIWKTNSLPLRFWVNILKNPHFIFDVHLTEVVDASLSVIAQTFMDACTKTEHKLTRESPSNKLLYAKEISTYKKMVDDYYKGIRQMVPVSDQDMNTHLAEISRSHTDKLNTQAALHQLYQYASKYYDGIISSLEEDPAAQSKQLTLRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	XM_009300283.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio plexin b2a (plxnb2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300283.2

ORF Insert Sequence:

ATGGCGTGGA GGGCCCTGGC AGCTTTCTCT CTACTACTGT GTTTTGGCTC ATCTTGCTGT 
GATGATGCTC AAGAACAGTT CTCGTCTCCT ACCATCATCA ATAATGTGGT CCAGGACCCT 
CAAACAGGCC GAATCTACCT TGGAGCTGTC AATGGGATCT TTCAGCTGAA CCACAATCTA 
CAGGAGGAGA GCAGAGCAGA CACAGGCCCT AAGAAAGACA ACCCACAATG CACTCCACCT 
ATCACTGCGC AGTGCACCGA TGCGAAGGAA ATGGACAACT TTAACAAACT CCTTCTGGTT 
AACTCCGCCA ACGGCACCTT GATCGTTTGT GGAAGCCTTT TCAGGGGCAT CTGCTCTTTG 
GTAAACCTCA ACAGTGTGAA TAAACCGGTG TACTACAGTG ACACCAAAGG AGAGAAGACA 
TATGTTGCCA GCACTGAGGA ATCTGTCACT GTGGTGGGGG TCATTTCTTA TTTTAAGGAC 
ACTACTACTA ATGCTAACCT CAGTGTATTT CTAGTGGGAA AAGGCTATGG AAGTTCGGAC 
AGCTCAAAGC TCATTAGCAC TCGGTTGCTG CAGGAACATG GAGAAATGGA TGTTTTTGAG 
AACATGGTTG AAGCTTCCAC TGTTCAAACG AGTGCGTTTG TCCTTCGGTA CCACCACGAT 
TTTCGCTACA CGTTTAAAGA CAATGGCTAC ATCTACTTCC TGTTCTCCCG CTCTCCTGGC 
ACTAGCGACA GCACGAAGAT TACATTTATA GCACGCCTTT GCGAGAACGA CCATCATTAT 
TATTCTTACA CAGAACTTCA GCTCAACTGC ACTGTTAACA CCGAACAACA AGAGAACACA 
TTCAACAAAG TCCAAGCAGC GTATCTGGCT AAACCTGGAA AGGATTTGGC TCAGAATATC 
GTCCCGTCAA ATTTAAATGA TAAGGTTCTC TTTGGAGTGT TCAGTGCAGA TGAGGCGGAC 
GGGCGTTCAG CATTGTGCAT GTACCCGCTT AGCAGCATTA ATGCCAGGTT TGAGGAGGTG 
ATTGAGTCCT GCTACACAGG AGAAGTTCTG GAGGATGACA AACCGAAGAC TGTTTATTCA 
CCCTACAACT CCAAGAATGA AGCCATATGC AAGACCAAAA GAGATAAGAA CATGGTAAAG 
GCATATATAT GTGGCGCAGA GTTTCTGCCA TCTCCACTAG CCAGTAAACC TGAGTATGCT 
TTAGCCGTCA AACCTATCTA CACCAGGAAT GACATGCTGA CAGCTGTAGC TGTGGCGGTG 
GAGAACGAAC ACACTGTGGC ATTCTTGGGA ACCAGTGGTG CAGACGTCTT GAAGGTGCAT 
CTTGACCCAA ACCACACTGA CTTTTACAAC AGAATTCCAG GGGATCGTGC AGATGGTGCA 
GTAAACAAAA ACCTTTTGTT TGATTCAACT TTGAATCACC TGTACATCAC CACTGGCAAA 
AAGATCTCCA AAGTGTCAGT GCAGGCCTGT GAGCAGAGAG AAGATTGTCA CTCATGTGTT 
TCCCAGCGGG ACCCATATTG CGGCTGGTGT GTGTTGGAGG GCCGCTGCAC CAGGAAAAAA 
GATTGTCGAA AAGGAGATGG AGAAAACGCC TGGTTGTGGA GTCCAAAACA AACATGTGTA 
ACCATTCAGT CCTTTGAGCC CCCCAACATC AGCTGCAAGA AAACAGATAG TACACAGGTG 
GTGATCAACA TCCCGTCCCT CCCGAGCATC AGCAGTCCTG ACAGGCTGGA GTGTGTGTTT 
GACGGCTACA GGAGTCAAGC AAAGATGGAG GACACTCGGG TGACCTGCTA CCTCCCCAGC 
TCGGACTACA TCCCACCAAC ACCCGAGACA CAAGATTTTG TGGCAGTGCC AGTAAAAATC 
ACAGTGAATG ACAAGATTGA GGTGGCATCG AGTGCGTTTG AGTTTTACAA CTGTGCTGCC 
GTGGTGAAAA AATCAGAGAA TACACCATGC ATTGCATGTG TGACCAGTAA GTGGGGCTGT 
CAGTGGAATA TTCAGGATCA CACATGCAGT GATAAAGATG ACTCTGCCCA TGGGAGTCAT 
ATCATCGGAA ACATGAAGGC TAATCTGTGT CCACAGTTTG AGATTCCTGA GCCTCTGCGT 
ATTCCTGTTG GTTATAAAAC CCCCATCACA TTTCATGGCA AAAATCTGGA TATTTATTGG 
GGCAAAACCT TTCAGATCGG CACCGAGCTC ATGAAGCAGG TGGGCGAAGT GACTGTGGAG 
GCCGATGGGT TAAAGTACAG ATTTGAGGGA TATGAGTTTG CGTATGACAA AGAGCCAGAG 
GTGAATGTGA CGTTCTACAT AAAGGATAAA GACACGGATA GGAAGATTGA CAGCACCCTG 
AGAGTGGTTC TCTACAACTG TTCGGTTAGA CGCGAGGACT GCAGTCTGTG TAAAAATGCA 
CACAAGAATG ATAGCTGCGT TTGGTGCGAC ACTACAAAAT CCTGCATCTA CAGAAGCCTG 
TGTGACAATG AACTGCACCA GTGTCCTCCT CCAAAGATCA CAGACATTGT TCCCCGTTAT 
GGGCCAATGA ACGGACGCAT CTCAGTCACA ATAAAAGGCT CAAATTTGGG CATCAAGAAG 
GAGGACATTA AGAAAATCAC TGTGGCTAAA GTTCCATGCA TTCATCAGCC AGAGCGATAC 
TCGGTTTCTA CTAGTGTTGT GTGTGAGATC GGCCCTTTCC CCTTACCTCC TGAAGATCTT 
CCCATTTCAC CGAGCAAATC CGGGCCGGTG GAGGTGTTGG TGGAAGGAGA AAGAACAGGG 
ACGTCCGAGG TGGAATTCAC TTACAGGGAT CCAGTCCCAG AAGCTGTGTT CCCCGTTAAA 
GGCCCAGCAG CTGGAGGAAC TGTCATCACA ATCACAGGAC AGGACCTCGA CTCGGCCAGT 
AAAGATGACA TCACCATTAC AGTGGACAAA GAGCCATGTG AAGTGTTGAC GTTTGGCCAT 
GAGATCACCT GCAAAACTGG CCCATATAAA GGACAAAAGA TTCCCTCGGA TAAGTTGCCT 
GTTATTGTGA AGTATGGCAA GGACACTGTT AAGACAGTCG ATGCATTCGA ATACTCACCC 
GATCCCAAAA TTTCTGAATA TTTTCCACAG ACCAGCTTTG TTTGCGGTGG TCGCAGGATC 
TTAGTGATAG GCTCTGGATT TGATCAGGTT CAGAGAGCCA CCATGCGAGT GCTGCCCTCT 
TCTGACGAGT TTGACGTCAA GGCTAGCAAT GTAGAGTTCT TTCAAGAAGT GGAAAAGAAG 
AACGAGACGA CGCTGGAGTT TCTGTCGCCG GTTGTCAATA AGACATACAT CAACCTGTCC 
TTAAGAACCG TCATCCAGCT GGACAACCTG GTGCTCAATC TGACAGATTT CGCTTACCAT 
CCGGACCCAC ACTTCGATAA ACTCAAAGAT AAAGCCATAA CAGAGGGCAG CATCATCAAT 
GTTAATGGTA GAGGTTTTGC CAAAGCCATG ACTGCCAAAG AGGCACAGGC ATTTGTGGGT 
GACGAGTCGT GTCTGGTGAC AATCCTTGAG GACGATAAAC TGTTCCTGGT GGCCCCGTCC 
AGCCCTCCAA AATCCACCTC CAGACGCCAT CGAAGAGACA CCAGCTCAGA TGCCTTGGAG 
CTGCTGATCA AGTTTGGAAA TGGAGAGTGG AATGTAGGCT CCGTGCGCTT CGAGAGGAAA 
TACGAGGTTC CCCTTTACAT CATAATCCCC GCTGTTCTCA TTCCCATGGT GCTCTTCATT 
GCGGTGTCCA TCTATTGCTA CAGGAGGAAA AGCCAGCAGG CAGAACGAGA ATATGAGAAG 
ATCAAAAATC AGCTGGCCAA TTTGGAGGAG AGCGTCCGTG ATCGCTGTAA AAAGGAGTTC 
ACAGATCTAA TGATAGAGAT GGAGGATCAC ACCAGTGATC TGAGTGAAGC TCGCATCCCA 
TTCCTGGACT ACAAGAACTA CACCGACCGT GTTTTCTTCC TGCCCTCGAA AGATGGAGCC 
AATGATGTCA TGATCACCGG CAAGCTGGAT ATCCCTGAAA ACCGCAGAGC CATCGTAGAT 
CAGGCACTCA ACCAGTTCTC CAACCTCCTG AACAGCAAAA CCTTCCTTAT CAATTTTATT 
CGTACCCTGG AGGGCCGACA GGAGTTCAAT GCCAGGAATA AGGTTTACTT CGCCTCTTTG 
TTGACGGTGG CACTGCATGG CAAGTTGGAG TATTACACGG ACATCATGAG GACTCTCCTG 
CTGGAGCTCA TGGAGGAATA TGTTCACAGC AAGAATCCCA AACTCATGCT CCGCAGATCC 
GAGACTGTTG TGGAGAGGAT GTTGTGCAAC TGGATGTCGA TTTGTCTCTA CCAGTTCCTG 
AAGGACTCAG CCGGCGAGCC GCTTTATAAG CTCTTTAAGG CCATTAAACA CCAGGTGGAA 
AAAGGCCCTG TTGATGCCAA GATGAAGAAA GCCAAGTACA CTCTCAACGA CACGGGGCTT 
CTGGGAGATG ATGTTGAGTA CAGCATTTTG ACCCTACAGG TGCTGGTTCA TGGCGAGGGT 
CCGGACGTGA CCCCGGTCAA AGTGCTAAAC TGCGACACCA TCTCGCAGGT GAAGGAGAAG 
ATCATCGAGC AGGTGTACAG GAATCTGCCA TATTCCCAGA GGCCCGTGGT GGACAGCGTC 
GCACTGGAGT GGCGTCCTGG TTCTACAGGG CAGATTCTGT CTGATCTGGA CCTGACCTCA 
CAGAAGGAGG GAAGGTGGAA ACGGATCAAC ACACTGGCAC ACTATAATGT GCGAGACGGA 
GCCACCCTGG TCTTATCCAG AGTCCTGCAC ACTCAGCAGT CCTTCGACCA GAACATGGAC 
AACCATGAAG AAAGAAATGC TTTGCTGGAG GATGATAAAG TGTTTCACTT GGTGAGACCG 
GCGGATGAGC TGGAGGAGGT CAAATCTAAG AGAGGGAGCA TGAAGGACAA GGCCACCACA 
AAAGCCATCA CTGAGATCTA CCTGACCAGA CTGCTCTCCA TGAAGGGAAC GCTGCAGCAG 
TTTGTAGATG ATTTCTTCCG CAGTGTGTTG TGTTCAGGGA CTGTGGTTCC TCCAGCCGTC 
AAGTACTTCT TTGACTTCTT AGATGAACAA GCTCAAAAAC ATGACAACGT TGATGATGAG 
ACCATCCACA TATGGAAGAC CAACAGTCTT CCTCTCCGTT TCTGGGTGAA CATCTTGAAG 
AATCCTCACT TCATCTTCGA TGTTCACCTG ACTGAGGTAG TGGACGCATC TCTGTCCGTC 
ATCGCTCAGA CCTTCATGGA CGCCTGCACT AAAACCGAGC ACAAACTGAC GCGGGAGTCT 
CCCAGTAACA AACTGCTTTA TGCAAAGGAA ATCTCCACGT ACAAGAAGAT GGTTGATGAT 
TATTATAAAG GCATTCGGCA GATGGTTCCA GTCAGTGACC AAGACATGAA CACACACCTC 
GCAGAGATTT CACGGTCTCA CACAGATAAG TTGAACACCC AGGCCGCGCT GCACCAGCTC 
TACCAGTATG CCAGCAAGTA CTATGACGGG ATCATCTCGT CACTGGAGGA AGATCCTGCC 
GCCCAGAGCA AACAACTGAC CCTCAGACTG CAACAGATCG CCGCTGCGCT GGAGAATAAA 
GTCACCGACC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLXNB2A ( XM_009300283.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298558.1 Danio rerio plexin b2a (plxnb2a), transcript variant X1, mRNA.

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CloneID	ODa43506
Clone ID Related Accession (Same CDS sequence)	XM_009300284.3 , XM_009300283.2 , XM_009300283.3 , XM_009300284.2
Accession Version	XM_009300283.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plexin-B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300283.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTGGA GGGCCCTGGC AGCTTTCTCT CTACTACTGT GTTTTGGCTC ATCTTGCTGT 
GATGATGCTC AAGAACAGTT CTCGTCTCCT ACCATCATCA ATAATGTGGT CCAGGACCCT 
CAAACAGGCC GAATCTACCT TGGAGCTGTC AATGGGATCT TTCAGCTGAA CCACAATCTA 
CAGGAGGAGA GCAGAGCAGA CACAGGCCCT AAGAAAGACA ACCCACAATG CACTCCACCT 
ATCACTGCGC AGTGCACCGA TGCGAAGGAA ATGGACAACT TTAACAAACT CCTTCTGGTT 
AACTCCGCCA ACGGCACCTT GATCGTTTGT GGAAGCCTTT TCAGGGGCAT CTGCTCTTTG 
GTAAACCTCA ACAGTGTGAA TAAACCGGTG TACTACAGTG ACACCAAAGG AGAGAAGACA 
TATGTTGCCA GCACTGAGGA ATCTGTCACT GTGGTGGGGG TCATTTCTTA TTTTAAGGAC 
ACTACTACTA ATGCTAACCT CAGTGTATTT CTAGTGGGAA AAGGCTATGG AAGTTCGGAC 
AGCTCAAAGC TCATTAGCAC TCGGTTGCTG CAGGAACATG GAGAAATGGA TGTTTTTGAG 
AACATGGTTG AAGCTTCCAC TGTTCAAACG AGTGCGTTTG TCCTTCGGTA CCACCACGAT 
TTTCGCTACA CGTTTAAAGA CAATGGCTAC ATCTACTTCC TGTTCTCCCG CTCTCCTGGC 
ACTAGCGACA GCACGAAGAT TACATTTATA GCACGCCTTT GCGAGAACGA CCATCATTAT 
TATTCTTACA CAGAACTTCA GCTCAACTGC ACTGTTAACA CCGAACAACA AGAGAACACA 
TTCAACAAAG TCCAAGCAGC GTATCTGGCT AAACCTGGAA AGGATTTGGC TCAGAATATC 
GTCCCGTCAA ATTTAAATGA TAAGGTTCTC TTTGGAGTGT TCAGTGCAGA TGAGGCGGAC 
GGGCGTTCAG CATTGTGCAT GTACCCGCTT AGCAGCATTA ATGCCAGGTT TGAGGAGGTG 
ATTGAGTCCT GCTACACAGG AGAAGTTCTG GAGGATGACA AACCGAAGAC TGTTTATTCA 
CCCTACAACT CCAAGAATGA AGCCATATGC AAGACCAAAA GAGATAAGAA CATGGTAAAG 
GCATATATAT GTGGCGCAGA GTTTCTGCCA TCTCCACTAG CCAGTAAACC TGAGTATGCT 
TTAGCCGTCA AACCTATCTA CACCAGGAAT GACATGCTGA CAGCTGTAGC TGTGGCGGTG 
GAGAACGAAC ACACTGTGGC ATTCTTGGGA ACCAGTGGTG CAGACGTCTT GAAGGTGCAT 
CTTGACCCAA ACCACACTGA CTTTTACAAC AGAATTCCAG GGGATCGTGC AGATGGTGCA 
GTAAACAAAA ACCTTTTGTT TGATTCAACT TTGAATCACC TGTACATCAC CACTGGCAAA 
AAGATCTCCA AAGTGTCAGT GCAGGCCTGT GAGCAGAGAG AAGATTGTCA CTCATGTGTT 
TCCCAGCGGG ACCCATATTG CGGCTGGTGT GTGTTGGAGG GCCGCTGCAC CAGGAAAAAA 
GATTGTCGAA AAGGAGATGG AGAAAACGCC TGGTTGTGGA GTCCAAAACA AACATGTGTA 
ACCATTCAGT CCTTTGAGCC CCCCAACATC AGCTGCAAGA AAACAGATAG TACACAGGTG 
GTGATCAACA TCCCGTCCCT CCCGAGCATC AGCAGTCCTG ACAGGCTGGA GTGTGTGTTT 
GACGGCTACA GGAGTCAAGC AAAGATGGAG GACACTCGGG TGACCTGCTA CCTCCCCAGC 
TCGGACTACA TCCCACCAAC ACCCGAGACA CAAGATTTTG TGGCAGTGCC AGTAAAAATC 
ACAGTGAATG ACAAGATTGA GGTGGCATCG AGTGCGTTTG AGTTTTACAA CTGTGCTGCC 
GTGGTGAAAA AATCAGAGAA TACACCATGC ATTGCATGTG TGACCAGTAA GTGGGGCTGT 
CAGTGGAATA TTCAGGATCA CACATGCAGT GATAAAGATG ACTCTGCCCA TGGGAGTCAT 
ATCATCGGAA ACATGAAGGC TAATCTGTGT CCACAGTTTG AGATTCCTGA GCCTCTGCGT 
ATTCCTGTTG GTTATAAAAC CCCCATCACA TTTCATGGCA AAAATCTGGA TATTTATTGG 
GGCAAAACCT TTCAGATCGG CACCGAGCTC ATGAAGCAGG TGGGCGAAGT GACTGTGGAG 
GCCGATGGGT TAAAGTACAG ATTTGAGGGA TATGAGTTTG CGTATGACAA AGAGCCAGAG 
GTGAATGTGA CGTTCTACAT AAAGGATAAA GACACGGATA GGAAGATTGA CAGCACCCTG 
AGAGTGGTTC TCTACAACTG TTCGGTTAGA CGCGAGGACT GCAGTCTGTG TAAAAATGCA 
CACAAGAATG ATAGCTGCGT TTGGTGCGAC ACTACAAAAT CCTGCATCTA CAGAAGCCTG 
TGTGACAATG AACTGCACCA GTGTCCTCCT CCAAAGATCA CAGACATTGT TCCCCGTTAT 
GGGCCAATGA ACGGACGCAT CTCAGTCACA ATAAAAGGCT CAAATTTGGG CATCAAGAAG 
GAGGACATTA AGAAAATCAC TGTGGCTAAA GTTCCATGCA TTCATCAGCC AGAGCGATAC 
TCGGTTTCTA CTAGTGTTGT GTGTGAGATC GGCCCTTTCC CCTTACCTCC TGAAGATCTT 
CCCATTTCAC CGAGCAAATC CGGGCCGGTG GAGGTGTTGG TGGAAGGAGA AAGAACAGGG 
ACGTCCGAGG TGGAATTCAC TTACAGGGAT CCAGTCCCAG AAGCTGTGTT CCCCGTTAAA 
GGCCCAGCAG CTGGAGGAAC TGTCATCACA ATCACAGGAC AGGACCTCGA CTCGGCCAGT 
AAAGATGACA TCACCATTAC AGTGGACAAA GAGCCATGTG AAGTGTTGAC GTTTGGCCAT 
GAGATCACCT GCAAAACTGG CCCATATAAA GGACAAAAGA TTCCCTCGGA TAAGTTGCCT 
GTTATTGTGA AGTATGGCAA GGACACTGTT AAGACAGTCG ATGCATTCGA ATACTCACCC 
GATCCCAAAA TTTCTGAATA TTTTCCACAG ACCAGCTTTG TTTGCGGTGG TCGCAGGATC 
TTAGTGATAG GCTCTGGATT TGATCAGGTT CAGAGAGCCA CCATGCGAGT GCTGCCCTCT 
TCTGACGAGT TTGACGTCAA GGCTAGCAAT GTAGAGTTCT TTCAAGAAGT GGAAAAGAAG 
AACGAGACGA CGCTGGAGTT TCTGTCGCCG GTTGTCAATA AGACATACAT CAACCTGTCC 
TTAAGAACCG TCATCCAGCT GGACAACCTG GTGCTCAATC TGACAGATTT CGCTTACCAT 
CCGGACCCAC ACTTCGATAA ACTCAAAGAT AAAGCCATAA CAGAGGGCAG CATCATCAAT 
GTTAATGGTA GAGGTTTTGC CAAAGCCATG ACTGCCAAAG AGGCACAGGC ATTTGTGGGT 
GACGAGTCGT GTCTGGTGAC AATCCTTGAG GACGATAAAC TGTTCCTGGT GGCCCCGTCC 
AGCCCTCCAA AATCCACCTC CAGACGCCAT CGAAGAGACA CCAGCTCAGA TGCCTTGGAG 
CTGCTGATCA AGTTTGGAAA TGGAGAGTGG AATGTAGGCT CCGTGCGCTT CGAGAGGAAA 
TACGAGGTTC CCCTTTACAT CATAATCCCC GCTGTTCTCA TTCCCATGGT GCTCTTCATT 
GCGGTGTCCA TCTATTGCTA CAGGAGGAAA AGCCAGCAGG CAGAACGAGA ATATGAGAAG 
ATCAAAAATC AGCTGGCCAA TTTGGAGGAG AGCGTCCGTG ATCGCTGTAA AAAGGAGTTC 
ACAGATCTAA TGATAGAGAT GGAGGATCAC ACCAGTGATC TGAGTGAAGC TCGCATCCCA 
TTCCTGGACT ACAAGAACTA CACCGACCGT GTTTTCTTCC TGCCCTCGAA AGATGGAGCC 
AATGATGTCA TGATCACCGG CAAGCTGGAT ATCCCTGAAA ACCGCAGAGC CATCGTAGAT 
CAGGCACTCA ACCAGTTCTC CAACCTCCTG AACAGCAAAA CCTTCCTTAT CAATTTTATT 
CGTACCCTGG AGGGCCGACA GGAGTTCAAT GCCAGGAATA AGGTTTACTT CGCCTCTTTG 
TTGACGGTGG CACTGCATGG CAAGTTGGAG TATTACACGG ACATCATGAG GACTCTCCTG 
CTGGAGCTCA TGGAGGAATA TGTTCACAGC AAGAATCCCA AACTCATGCT CCGCAGATCC 
GAGACTGTTG TGGAGAGGAT GTTGTGCAAC TGGATGTCGA TTTGTCTCTA CCAGTTCCTG 
AAGGACTCAG CCGGCGAGCC GCTTTATAAG CTCTTTAAGG CCATTAAACA CCAGGTGGAA 
AAAGGCCCTG TTGATGCCAA GATGAAGAAA GCCAAGTACA CTCTCAACGA CACGGGGCTT 
CTGGGAGATG ATGTTGAGTA CAGCATTTTG ACCCTACAGG TGCTGGTTCA TGGCGAGGGT 
CCGGACGTGA CCCCGGTCAA AGTGCTAAAC TGCGACACCA TCTCGCAGGT GAAGGAGAAG 
ATCATCGAGC AGGTGTACAG GAATCTGCCA TATTCCCAGA GGCCCGTGGT GGACAGCGTC 
GCACTGGAGT GGCGTCCTGG TTCTACAGGG CAGATTCTGT CTGATCTGGA CCTGACCTCA 
CAGAAGGAGG GAAGGTGGAA ACGGATCAAC ACACTGGCAC ACTATAATGT GCGAGACGGA 
GCCACCCTGG TCTTATCCAG AGTCCTGCAC ACTCAGCAGT CCTTCGACCA GAACATGGAC 
AACCATGAAG AAAGAAATGC TTTGCTGGAG GATGATAAAG TGTTTCACTT GGTGAGACCG 
GCGGATGAGC TGGAGGAGGT CAAATCTAAG AGAGGGAGCA TGAAGGACAA GGCCACCACA 
AAAGCCATCA CTGAGATCTA CCTGACCAGA CTGCTCTCCA TGAAGGGAAC GCTGCAGCAG 
TTTGTAGATG ATTTCTTCCG CAGTGTGTTG TGTTCAGGGA CTGTGGTTCC TCCAGCCGTC 
AAGTACTTCT TTGACTTCTT AGATGAACAA GCTCAAAAAC ATGACAACGT TGATGATGAG 
ACCATCCACA TATGGAAGAC CAACAGTCTT CCTCTCCGTT TCTGGGTGAA CATCTTGAAG 
AATCCTCACT TCATCTTCGA TGTTCACCTG ACTGAGGTAG TGGACGCATC TCTGTCCGTC 
ATCGCTCAGA CCTTCATGGA CGCCTGCACT AAAACCGAGC ACAAACTGAC GCGGGAGTCT 
CCCAGTAACA AACTGCTTTA TGCAAAGGAA ATCTCCACGT ACAAGAAGAT GGTTGATGAT 
TATTATAAAG GCATTCGGCA GATGGTTCCA GTCAGTGACC AAGACATGAA CACACACCTC 
GCAGAGATTT CACGGTCTCA CACAGATAAG TTGAACACCC AGGCCGCGCT GCACCAGCTC 
TACCAGTATG CCAGCAAGTA CTATGACGGG ATCATCTCGT CACTGGAGGA AGATCCTGCC 
GCCCAGAGCA AACAACTGAC CCTCAGACTG CAACAGATCG CCGCTGCGCT GGAGAATAAA 
GTCACCGACC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298558.1
CDS	678..6272
Translation	MAWRALAAFSLLLCFGSSCCDDAQEQFSSPTIINNVVQDPQTGRIYLGAVNGIFQLNHNLQEESRADTGPKKDNPQCTPPITAQCTDAKEMDNFNKLLLVNSANGTLIVCGSLFRGICSLVNLNSVNKPVYYSDTKGEKTYVASTEESVTVVGVISYFKDTTTNANLSVFLVGKGYGSSDSSKLISTRLLQEHGEMDVFENMVEASTVQTSAFVLRYHHDFRYTFKDNGYIYFLFSRSPGTSDSTKITFIARLCENDHHYYSYTELQLNCTVNTEQQENTFNKVQAAYLAKPGKDLAQNIVPSNLNDKVLFGVFSADEADGRSALCMYPLSSINARFEEVIESCYTGEVLEDDKPKTVYSPYNSKNEAICKTKRDKNMVKAYICGAEFLPSPLASKPEYALAVKPIYTRNDMLTAVAVAVENEHTVAFLGTSGADVLKVHLDPNHTDFYNRIPGDRADGAVNKNLLFDSTLNHLYITTGKKISKVSVQACEQREDCHSCVSQRDPYCGWCVLEGRCTRKKDCRKGDGENAWLWSPKQTCVTIQSFEPPNISCKKTDSTQVVINIPSLPSISSPDRLECVFDGYRSQAKMEDTRVTCYLPSSDYIPPTPETQDFVAVPVKITVNDKIEVASSAFEFYNCAAVVKKSENTPCIACVTSKWGCQWNIQDHTCSDKDDSAHGSHIIGNMKANLCPQFEIPEPLRIPVGYKTPITFHGKNLDIYWGKTFQIGTELMKQVGEVTVEADGLKYRFEGYEFAYDKEPEVNVTFYIKDKDTDRKIDSTLRVVLYNCSVRREDCSLCKNAHKNDSCVWCDTTKSCIYRSLCDNELHQCPPPKITDIVPRYGPMNGRISVTIKGSNLGIKKEDIKKITVAKVPCIHQPERYSVSTSVVCEIGPFPLPPEDLPISPSKSGPVEVLVEGERTGTSEVEFTYRDPVPEAVFPVKGPAAGGTVITITGQDLDSASKDDITITVDKEPCEVLTFGHEITCKTGPYKGQKIPSDKLPVIVKYGKDTVKTVDAFEYSPDPKISEYFPQTSFVCGGRRILVIGSGFDQVQRATMRVLPSSDEFDVKASNVEFFQEVEKKNETTLEFLSPVVNKTYINLSLRTVIQLDNLVLNLTDFAYHPDPHFDKLKDKAITEGSIINVNGRGFAKAMTAKEAQAFVGDESCLVTILEDDKLFLVAPSSPPKSTSRRHRRDTSSDALELLIKFGNGEWNVGSVRFERKYEVPLYIIIPAVLIPMVLFIAVSIYCYRRKSQQAEREYEKIKNQLANLEESVRDRCKKEFTDLMIEMEDHTSDLSEARIPFLDYKNYTDRVFFLPSKDGANDVMITGKLDIPENRRAIVDQALNQFSNLLNSKTFLINFIRTLEGRQEFNARNKVYFASLLTVALHGKLEYYTDIMRTLLLELMEEYVHSKNPKLMLRRSETVVERMLCNWMSICLYQFLKDSAGEPLYKLFKAIKHQVEKGPVDAKMKKAKYTLNDTGLLGDDVEYSILTLQVLVHGEGPDVTPVKVLNCDTISQVKEKIIEQVYRNLPYSQRPVVDSVALEWRPGSTGQILSDLDLTSQKEGRWKRINTLAHYNVRDGATLVLSRVLHTQQSFDQNMDNHEERNALLEDDKVFHLVRPADELEEVKSKRGSMKDKATTKAITEIYLTRLLSMKGTLQQFVDDFFRSVLCSGTVVPPAVKYFFDFLDEQAQKHDNVDDETIHIWKTNSLPLRFWVNILKNPHFIFDVHLTEVVDASLSVIAQTFMDACTKTEHKLTRESPSNKLLYAKEISTYKKMVDDYYKGIRQMVPVSDQDMNTHLAEISRSHTDKLNTQAALHQLYQYASKYYDGIISSLEEDPAAQSKQLTLRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	XM_009300283.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio plexin b2a (plxnb2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300283.3

ORF Insert Sequence:

ATGGCGTGGA GGGCCCTGGC AGCTTTCTCT CTACTACTGT GTTTTGGCTC ATCTTGCTGT 
GATGATGCTC AAGAACAGTT CTCGTCTCCT ACCATCATCA ATAATGTGGT CCAGGACCCT 
CAAACAGGCC GAATCTACCT TGGAGCTGTC AATGGGATCT TTCAGCTGAA CCACAATCTA 
CAGGAGGAGA GCAGAGCAGA CACAGGCCCT AAGAAAGACA ACCCACAATG CACTCCACCT 
ATCACTGCGC AGTGCACCGA TGCGAAGGAA ATGGACAACT TTAACAAACT CCTTCTGGTT 
AACTCCGCCA ACGGCACCTT GATCGTTTGT GGAAGCCTTT TCAGGGGCAT CTGCTCTTTG 
GTAAACCTCA ACAGTGTGAA TAAACCGGTG TACTACAGTG ACACCAAAGG AGAGAAGACA 
TATGTTGCCA GCACTGAGGA ATCTGTCACT GTGGTGGGGG TCATTTCTTA TTTTAAGGAC 
ACTACTACTA ATGCTAACCT CAGTGTATTT CTAGTGGGAA AAGGCTATGG AAGTTCGGAC 
AGCTCAAAGC TCATTAGCAC TCGGTTGCTG CAGGAACATG GAGAAATGGA TGTTTTTGAG 
AACATGGTTG AAGCTTCCAC TGTTCAAACG AGTGCGTTTG TCCTTCGGTA CCACCACGAT 
TTTCGCTACA CGTTTAAAGA CAATGGCTAC ATCTACTTCC TGTTCTCCCG CTCTCCTGGC 
ACTAGCGACA GCACGAAGAT TACATTTATA GCACGCCTTT GCGAGAACGA CCATCATTAT 
TATTCTTACA CAGAACTTCA GCTCAACTGC ACTGTTAACA CCGAACAACA AGAGAACACA 
TTCAACAAAG TCCAAGCAGC GTATCTGGCT AAACCTGGAA AGGATTTGGC TCAGAATATC 
GTCCCGTCAA ATTTAAATGA TAAGGTTCTC TTTGGAGTGT TCAGTGCAGA TGAGGCGGAC 
GGGCGTTCAG CATTGTGCAT GTACCCGCTT AGCAGCATTA ATGCCAGGTT TGAGGAGGTG 
ATTGAGTCCT GCTACACAGG AGAAGTTCTG GAGGATGACA AACCGAAGAC TGTTTATTCA 
CCCTACAACT CCAAGAATGA AGCCATATGC AAGACCAAAA GAGATAAGAA CATGGTAAAG 
GCATATATAT GTGGCGCAGA GTTTCTGCCA TCTCCACTAG CCAGTAAACC TGAGTATGCT 
TTAGCCGTCA AACCTATCTA CACCAGGAAT GACATGCTGA CAGCTGTAGC TGTGGCGGTG 
GAGAACGAAC ACACTGTGGC ATTCTTGGGA ACCAGTGGTG CAGACGTCTT GAAGGTGCAT 
CTTGACCCAA ACCACACTGA CTTTTACAAC AGAATTCCAG GGGATCGTGC AGATGGTGCA 
GTAAACAAAA ACCTTTTGTT TGATTCAACT TTGAATCACC TGTACATCAC CACTGGCAAA 
AAGATCTCCA AAGTGTCAGT GCAGGCCTGT GAGCAGAGAG AAGATTGTCA CTCATGTGTT 
TCCCAGCGGG ACCCATATTG CGGCTGGTGT GTGTTGGAGG GCCGCTGCAC CAGGAAAAAA 
GATTGTCGAA AAGGAGATGG AGAAAACGCC TGGTTGTGGA GTCCAAAACA AACATGTGTA 
ACCATTCAGT CCTTTGAGCC CCCCAACATC AGCTGCAAGA AAACAGATAG TACACAGGTG 
GTGATCAACA TCCCGTCCCT CCCGAGCATC AGCAGTCCTG ACAGGCTGGA GTGTGTGTTT 
GACGGCTACA GGAGTCAAGC AAAGATGGAG GACACTCGGG TGACCTGCTA CCTCCCCAGC 
TCGGACTACA TCCCACCAAC ACCCGAGACA CAAGATTTTG TGGCAGTGCC AGTAAAAATC 
ACAGTGAATG ACAAGATTGA GGTGGCATCG AGTGCGTTTG AGTTTTACAA CTGTGCTGCC 
GTGGTGAAAA AATCAGAGAA TACACCATGC ATTGCATGTG TGACCAGTAA GTGGGGCTGT 
CAGTGGAATA TTCAGGATCA CACATGCAGT GATAAAGATG ACTCTGCCCA TGGGAGTCAT 
ATCATCGGAA ACATGAAGGC TAATCTGTGT CCACAGTTTG AGATTCCTGA GCCTCTGCGT 
ATTCCTGTTG GTTATAAAAC CCCCATCACA TTTCATGGCA AAAATCTGGA TATTTATTGG 
GGCAAAACCT TTCAGATCGG CACCGAGCTC ATGAAGCAGG TGGGCGAAGT GACTGTGGAG 
GCCGATGGGT TAAAGTACAG ATTTGAGGGA TATGAGTTTG CGTATGACAA AGAGCCAGAG 
GTGAATGTGA CGTTCTACAT AAAGGATAAA GACACGGATA GGAAGATTGA CAGCACCCTG 
AGAGTGGTTC TCTACAACTG TTCGGTTAGA CGCGAGGACT GCAGTCTGTG TAAAAATGCA 
CACAAGAATG ATAGCTGCGT TTGGTGCGAC ACTACAAAAT CCTGCATCTA CAGAAGCCTG 
TGTGACAATG AACTGCACCA GTGTCCTCCT CCAAAGATCA CAGACATTGT TCCCCGTTAT 
GGGCCAATGA ACGGACGCAT CTCAGTCACA ATAAAAGGCT CAAATTTGGG CATCAAGAAG 
GAGGACATTA AGAAAATCAC TGTGGCTAAA GTTCCATGCA TTCATCAGCC AGAGCGATAC 
TCGGTTTCTA CTAGTGTTGT GTGTGAGATC GGCCCTTTCC CCTTACCTCC TGAAGATCTT 
CCCATTTCAC CGAGCAAATC CGGGCCGGTG GAGGTGTTGG TGGAAGGAGA AAGAACAGGG 
ACGTCCGAGG TGGAATTCAC TTACAGGGAT CCAGTCCCAG AAGCTGTGTT CCCCGTTAAA 
GGCCCAGCAG CTGGAGGAAC TGTCATCACA ATCACAGGAC AGGACCTCGA CTCGGCCAGT 
AAAGATGACA TCACCATTAC AGTGGACAAA GAGCCATGTG AAGTGTTGAC GTTTGGCCAT 
GAGATCACCT GCAAAACTGG CCCATATAAA GGACAAAAGA TTCCCTCGGA TAAGTTGCCT 
GTTATTGTGA AGTATGGCAA GGACACTGTT AAGACAGTCG ATGCATTCGA ATACTCACCC 
GATCCCAAAA TTTCTGAATA TTTTCCACAG ACCAGCTTTG TTTGCGGTGG TCGCAGGATC 
TTAGTGATAG GCTCTGGATT TGATCAGGTT CAGAGAGCCA CCATGCGAGT GCTGCCCTCT 
TCTGACGAGT TTGACGTCAA GGCTAGCAAT GTAGAGTTCT TTCAAGAAGT GGAAAAGAAG 
AACGAGACGA CGCTGGAGTT TCTGTCGCCG GTTGTCAATA AGACATACAT CAACCTGTCC 
TTAAGAACCG TCATCCAGCT GGACAACCTG GTGCTCAATC TGACAGATTT CGCTTACCAT 
CCGGACCCAC ACTTCGATAA ACTCAAAGAT AAAGCCATAA CAGAGGGCAG CATCATCAAT 
GTTAATGGTA GAGGTTTTGC CAAAGCCATG ACTGCCAAAG AGGCACAGGC ATTTGTGGGT 
GACGAGTCGT GTCTGGTGAC AATCCTTGAG GACGATAAAC TGTTCCTGGT GGCCCCGTCC 
AGCCCTCCAA AATCCACCTC CAGACGCCAT CGAAGAGACA CCAGCTCAGA TGCCTTGGAG 
CTGCTGATCA AGTTTGGAAA TGGAGAGTGG AATGTAGGCT CCGTGCGCTT CGAGAGGAAA 
TACGAGGTTC CCCTTTACAT CATAATCCCC GCTGTTCTCA TTCCCATGGT GCTCTTCATT 
GCGGTGTCCA TCTATTGCTA CAGGAGGAAA AGCCAGCAGG CAGAACGAGA ATATGAGAAG 
ATCAAAAATC AGCTGGCCAA TTTGGAGGAG AGCGTCCGTG ATCGCTGTAA AAAGGAGTTC 
ACAGATCTAA TGATAGAGAT GGAGGATCAC ACCAGTGATC TGAGTGAAGC TCGCATCCCA 
TTCCTGGACT ACAAGAACTA CACCGACCGT GTTTTCTTCC TGCCCTCGAA AGATGGAGCC 
AATGATGTCA TGATCACCGG CAAGCTGGAT ATCCCTGAAA ACCGCAGAGC CATCGTAGAT 
CAGGCACTCA ACCAGTTCTC CAACCTCCTG AACAGCAAAA CCTTCCTTAT CAATTTTATT 
CGTACCCTGG AGGGCCGACA GGAGTTCAAT GCCAGGAATA AGGTTTACTT CGCCTCTTTG 
TTGACGGTGG CACTGCATGG CAAGTTGGAG TATTACACGG ACATCATGAG GACTCTCCTG 
CTGGAGCTCA TGGAGGAATA TGTTCACAGC AAGAATCCCA AACTCATGCT CCGCAGATCC 
GAGACTGTTG TGGAGAGGAT GTTGTGCAAC TGGATGTCGA TTTGTCTCTA CCAGTTCCTG 
AAGGACTCAG CCGGCGAGCC GCTTTATAAG CTCTTTAAGG CCATTAAACA CCAGGTGGAA 
AAAGGCCCTG TTGATGCCAA GATGAAGAAA GCCAAGTACA CTCTCAACGA CACGGGGCTT 
CTGGGAGATG ATGTTGAGTA CAGCATTTTG ACCCTACAGG TGCTGGTTCA TGGCGAGGGT 
CCGGACGTGA CCCCGGTCAA AGTGCTAAAC TGCGACACCA TCTCGCAGGT GAAGGAGAAG 
ATCATCGAGC AGGTGTACAG GAATCTGCCA TATTCCCAGA GGCCCGTGGT GGACAGCGTC 
GCACTGGAGT GGCGTCCTGG TTCTACAGGG CAGATTCTGT CTGATCTGGA CCTGACCTCA 
CAGAAGGAGG GAAGGTGGAA ACGGATCAAC ACACTGGCAC ACTATAATGT GCGAGACGGA 
GCCACCCTGG TCTTATCCAG AGTCCTGCAC ACTCAGCAGT CCTTCGACCA GAACATGGAC 
AACCATGAAG AAAGAAATGC TTTGCTGGAG GATGATAAAG TGTTTCACTT GGTGAGACCG 
GCGGATGAGC TGGAGGAGGT CAAATCTAAG AGAGGGAGCA TGAAGGACAA GGCCACCACA 
AAAGCCATCA CTGAGATCTA CCTGACCAGA CTGCTCTCCA TGAAGGGAAC GCTGCAGCAG 
TTTGTAGATG ATTTCTTCCG CAGTGTGTTG TGTTCAGGGA CTGTGGTTCC TCCAGCCGTC 
AAGTACTTCT TTGACTTCTT AGATGAACAA GCTCAAAAAC ATGACAACGT TGATGATGAG 
ACCATCCACA TATGGAAGAC CAACAGTCTT CCTCTCCGTT TCTGGGTGAA CATCTTGAAG 
AATCCTCACT TCATCTTCGA TGTTCACCTG ACTGAGGTAG TGGACGCATC TCTGTCCGTC 
ATCGCTCAGA CCTTCATGGA CGCCTGCACT AAAACCGAGC ACAAACTGAC GCGGGAGTCT 
CCCAGTAACA AACTGCTTTA TGCAAAGGAA ATCTCCACGT ACAAGAAGAT GGTTGATGAT 
TATTATAAAG GCATTCGGCA GATGGTTCCA GTCAGTGACC AAGACATGAA CACACACCTC 
GCAGAGATTT CACGGTCTCA CACAGATAAG TTGAACACCC AGGCCGCGCT GCACCAGCTC 
TACCAGTATG CCAGCAAGTA CTATGACGGG ATCATCTCGT CACTGGAGGA AGATCCTGCC 
GCCCAGAGCA AACAACTGAC CCTCAGACTG CAACAGATCG CCGCTGCGCT GGAGAATAAA 
GTCACCGACC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLXNB2A ( XM_009300284.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298559.1 Danio rerio plexin b2a (plxnb2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43506
Clone ID Related Accession (Same CDS sequence)	XM_009300284.3 , XM_009300283.2 , XM_009300283.3 , XM_009300284.2
Accession Version	XM_009300284.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plexin-B2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTGGA GGGCCCTGGC AGCTTTCTCT CTACTACTGT GTTTTGGCTC ATCTTGCTGT 
GATGATGCTC AAGAACAGTT CTCGTCTCCT ACCATCATCA ATAATGTGGT CCAGGACCCT 
CAAACAGGCC GAATCTACCT TGGAGCTGTC AATGGGATCT TTCAGCTGAA CCACAATCTA 
CAGGAGGAGA GCAGAGCAGA CACAGGCCCT AAGAAAGACA ACCCACAATG CACTCCACCT 
ATCACTGCGC AGTGCACCGA TGCGAAGGAA ATGGACAACT TTAACAAACT CCTTCTGGTT 
AACTCCGCCA ACGGCACCTT GATCGTTTGT GGAAGCCTTT TCAGGGGCAT CTGCTCTTTG 
GTAAACCTCA ACAGTGTGAA TAAACCGGTG TACTACAGTG ACACCAAAGG AGAGAAGACA 
TATGTTGCCA GCACTGAGGA ATCTGTCACT GTGGTGGGGG TCATTTCTTA TTTTAAGGAC 
ACTACTACTA ATGCTAACCT CAGTGTATTT CTAGTGGGAA AAGGCTATGG AAGTTCGGAC 
AGCTCAAAGC TCATTAGCAC TCGGTTGCTG CAGGAACATG GAGAAATGGA TGTTTTTGAG 
AACATGGTTG AAGCTTCCAC TGTTCAAACG AGTGCGTTTG TCCTTCGGTA CCACCACGAT 
TTTCGCTACA CGTTTAAAGA CAATGGCTAC ATCTACTTCC TGTTCTCCCG CTCTCCTGGC 
ACTAGCGACA GCACGAAGAT TACATTTATA GCACGCCTTT GCGAGAACGA CCATCATTAT 
TATTCTTACA CAGAACTTCA GCTCAACTGC ACTGTTAACA CCGAACAACA AGAGAACACA 
TTCAACAAAG TCCAAGCAGC GTATCTGGCT AAACCTGGAA AGGATTTGGC TCAGAATATC 
GTCCCGTCAA ATTTAAATGA TAAGGTTCTC TTTGGAGTGT TCAGTGCAGA TGAGGCGGAC 
GGGCGTTCAG CATTGTGCAT GTACCCGCTT AGCAGCATTA ATGCCAGGTT TGAGGAGGTG 
ATTGAGTCCT GCTACACAGG AGAAGTTCTG GAGGATGACA AACCGAAGAC TGTTTATTCA 
CCCTACAACT CCAAGAATGA AGCCATATGC AAGACCAAAA GAGATAAGAA CATGGTAAAG 
GCATATATAT GTGGCGCAGA GTTTCTGCCA TCTCCACTAG CCAGTAAACC TGAGTATGCT 
TTAGCCGTCA AACCTATCTA CACCAGGAAT GACATGCTGA CAGCTGTAGC TGTGGCGGTG 
GAGAACGAAC ACACTGTGGC ATTCTTGGGA ACCAGTGGTG CAGACGTCTT GAAGGTGCAT 
CTTGACCCAA ACCACACTGA CTTTTACAAC AGAATTCCAG GGGATCGTGC AGATGGTGCA 
GTAAACAAAA ACCTTTTGTT TGATTCAACT TTGAATCACC TGTACATCAC CACTGGCAAA 
AAGATCTCCA AAGTGTCAGT GCAGGCCTGT GAGCAGAGAG AAGATTGTCA CTCATGTGTT 
TCCCAGCGGG ACCCATATTG CGGCTGGTGT GTGTTGGAGG GCCGCTGCAC CAGGAAAAAA 
GATTGTCGAA AAGGAGATGG AGAAAACGCC TGGTTGTGGA GTCCAAAACA AACATGTGTA 
ACCATTCAGT CCTTTGAGCC CCCCAACATC AGCTGCAAGA AAACAGATAG TACACAGGTG 
GTGATCAACA TCCCGTCCCT CCCGAGCATC AGCAGTCCTG ACAGGCTGGA GTGTGTGTTT 
GACGGCTACA GGAGTCAAGC AAAGATGGAG GACACTCGGG TGACCTGCTA CCTCCCCAGC 
TCGGACTACA TCCCACCAAC ACCCGAGACA CAAGATTTTG TGGCAGTGCC AGTAAAAATC 
ACAGTGAATG ACAAGATTGA GGTGGCATCG AGTGCGTTTG AGTTTTACAA CTGTGCTGCC 
GTGGTGAAAA AATCAGAGAA TACACCATGC ATTGCATGTG TGACCAGTAA GTGGGGCTGT 
CAGTGGAATA TTCAGGATCA CACATGCAGT GATAAAGATG ACTCTGCCCA TGGGAGTCAT 
ATCATCGGAA ACATGAAGGC TAATCTGTGT CCACAGTTTG AGATTCCTGA GCCTCTGCGT 
ATTCCTGTTG GTTATAAAAC CCCCATCACA TTTCATGGCA AAAATCTGGA TATTTATTGG 
GGCAAAACCT TTCAGATCGG CACCGAGCTC ATGAAGCAGG TGGGCGAAGT GACTGTGGAG 
GCCGATGGGT TAAAGTACAG ATTTGAGGGA TATGAGTTTG CGTATGACAA AGAGCCAGAG 
GTGAATGTGA CGTTCTACAT AAAGGATAAA GACACGGATA GGAAGATTGA CAGCACCCTG 
AGAGTGGTTC TCTACAACTG TTCGGTTAGA CGCGAGGACT GCAGTCTGTG TAAAAATGCA 
CACAAGAATG ATAGCTGCGT TTGGTGCGAC ACTACAAAAT CCTGCATCTA CAGAAGCCTG 
TGTGACAATG AACTGCACCA GTGTCCTCCT CCAAAGATCA CAGACATTGT TCCCCGTTAT 
GGGCCAATGA ACGGACGCAT CTCAGTCACA ATAAAAGGCT CAAATTTGGG CATCAAGAAG 
GAGGACATTA AGAAAATCAC TGTGGCTAAA GTTCCATGCA TTCATCAGCC AGAGCGATAC 
TCGGTTTCTA CTAGTGTTGT GTGTGAGATC GGCCCTTTCC CCTTACCTCC TGAAGATCTT 
CCCATTTCAC CGAGCAAATC CGGGCCGGTG GAGGTGTTGG TGGAAGGAGA AAGAACAGGG 
ACGTCCGAGG TGGAATTCAC TTACAGGGAT CCAGTCCCAG AAGCTGTGTT CCCCGTTAAA 
GGCCCAGCAG CTGGAGGAAC TGTCATCACA ATCACAGGAC AGGACCTCGA CTCGGCCAGT 
AAAGATGACA TCACCATTAC AGTGGACAAA GAGCCATGTG AAGTGTTGAC GTTTGGCCAT 
GAGATCACCT GCAAAACTGG CCCATATAAA GGACAAAAGA TTCCCTCGGA TAAGTTGCCT 
GTTATTGTGA AGTATGGCAA GGACACTGTT AAGACAGTCG ATGCATTCGA ATACTCACCC 
GATCCCAAAA TTTCTGAATA TTTTCCACAG ACCAGCTTTG TTTGCGGTGG TCGCAGGATC 
TTAGTGATAG GCTCTGGATT TGATCAGGTT CAGAGAGCCA CCATGCGAGT GCTGCCCTCT 
TCTGACGAGT TTGACGTCAA GGCTAGCAAT GTAGAGTTCT TTCAAGAAGT GGAAAAGAAG 
AACGAGACGA CGCTGGAGTT TCTGTCGCCG GTTGTCAATA AGACATACAT CAACCTGTCC 
TTAAGAACCG TCATCCAGCT GGACAACCTG GTGCTCAATC TGACAGATTT CGCTTACCAT 
CCGGACCCAC ACTTCGATAA ACTCAAAGAT AAAGCCATAA CAGAGGGCAG CATCATCAAT 
GTTAATGGTA GAGGTTTTGC CAAAGCCATG ACTGCCAAAG AGGCACAGGC ATTTGTGGGT 
GACGAGTCGT GTCTGGTGAC AATCCTTGAG GACGATAAAC TGTTCCTGGT GGCCCCGTCC 
AGCCCTCCAA AATCCACCTC CAGACGCCAT CGAAGAGACA CCAGCTCAGA TGCCTTGGAG 
CTGCTGATCA AGTTTGGAAA TGGAGAGTGG AATGTAGGCT CCGTGCGCTT CGAGAGGAAA 
TACGAGGTTC CCCTTTACAT CATAATCCCC GCTGTTCTCA TTCCCATGGT GCTCTTCATT 
GCGGTGTCCA TCTATTGCTA CAGGAGGAAA AGCCAGCAGG CAGAACGAGA ATATGAGAAG 
ATCAAAAATC AGCTGGCCAA TTTGGAGGAG AGCGTCCGTG ATCGCTGTAA AAAGGAGTTC 
ACAGATCTAA TGATAGAGAT GGAGGATCAC ACCAGTGATC TGAGTGAAGC TCGCATCCCA 
TTCCTGGACT ACAAGAACTA CACCGACCGT GTTTTCTTCC TGCCCTCGAA AGATGGAGCC 
AATGATGTCA TGATCACCGG CAAGCTGGAT ATCCCTGAAA ACCGCAGAGC CATCGTAGAT 
CAGGCACTCA ACCAGTTCTC CAACCTCCTG AACAGCAAAA CCTTCCTTAT CAATTTTATT 
CGTACCCTGG AGGGCCGACA GGAGTTCAAT GCCAGGAATA AGGTTTACTT CGCCTCTTTG 
TTGACGGTGG CACTGCATGG CAAGTTGGAG TATTACACGG ACATCATGAG GACTCTCCTG 
CTGGAGCTCA TGGAGGAATA TGTTCACAGC AAGAATCCCA AACTCATGCT CCGCAGATCC 
GAGACTGTTG TGGAGAGGAT GTTGTGCAAC TGGATGTCGA TTTGTCTCTA CCAGTTCCTG 
AAGGACTCAG CCGGCGAGCC GCTTTATAAG CTCTTTAAGG CCATTAAACA CCAGGTGGAA 
AAAGGCCCTG TTGATGCCAA GATGAAGAAA GCCAAGTACA CTCTCAACGA CACGGGGCTT 
CTGGGAGATG ATGTTGAGTA CAGCATTTTG ACCCTACAGG TGCTGGTTCA TGGCGAGGGT 
CCGGACGTGA CCCCGGTCAA AGTGCTAAAC TGCGACACCA TCTCGCAGGT GAAGGAGAAG 
ATCATCGAGC AGGTGTACAG GAATCTGCCA TATTCCCAGA GGCCCGTGGT GGACAGCGTC 
GCACTGGAGT GGCGTCCTGG TTCTACAGGG CAGATTCTGT CTGATCTGGA CCTGACCTCA 
CAGAAGGAGG GAAGGTGGAA ACGGATCAAC ACACTGGCAC ACTATAATGT GCGAGACGGA 
GCCACCCTGG TCTTATCCAG AGTCCTGCAC ACTCAGCAGT CCTTCGACCA GAACATGGAC 
AACCATGAAG AAAGAAATGC TTTGCTGGAG GATGATAAAG TGTTTCACTT GGTGAGACCG 
GCGGATGAGC TGGAGGAGGT CAAATCTAAG AGAGGGAGCA TGAAGGACAA GGCCACCACA 
AAAGCCATCA CTGAGATCTA CCTGACCAGA CTGCTCTCCA TGAAGGGAAC GCTGCAGCAG 
TTTGTAGATG ATTTCTTCCG CAGTGTGTTG TGTTCAGGGA CTGTGGTTCC TCCAGCCGTC 
AAGTACTTCT TTGACTTCTT AGATGAACAA GCTCAAAAAC ATGACAACGT TGATGATGAG 
ACCATCCACA TATGGAAGAC CAACAGTCTT CCTCTCCGTT TCTGGGTGAA CATCTTGAAG 
AATCCTCACT TCATCTTCGA TGTTCACCTG ACTGAGGTAG TGGACGCATC TCTGTCCGTC 
ATCGCTCAGA CCTTCATGGA CGCCTGCACT AAAACCGAGC ACAAACTGAC GCGGGAGTCT 
CCCAGTAACA AACTGCTTTA TGCAAAGGAA ATCTCCACGT ACAAGAAGAT GGTTGATGAT 
TATTATAAAG GCATTCGGCA GATGGTTCCA GTCAGTGACC AAGACATGAA CACACACCTC 
GCAGAGATTT CACGGTCTCA CACAGATAAG TTGAACACCC AGGCCGCGCT GCACCAGCTC 
TACCAGTATG CCAGCAAGTA CTATGACGGG ATCATCTCGT CACTGGAGGA AGATCCTGCC 
GCCCAGAGCA AACAACTGAC CCTCAGACTG CAACAGATCG CCGCTGCGCT GGAGAATAAA 
GTCACCGACC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298559.1
CDS	527..6121
Translation	MAWRALAAFSLLLCFGSSCCDDAQEQFSSPTIINNVVQDPQTGRIYLGAVNGIFQLNHNLQEESRADTGPKKDNPQCTPPITAQCTDAKEMDNFNKLLLVNSANGTLIVCGSLFRGICSLVNLNSVNKPVYYSDTKGEKTYVASTEESVTVVGVISYFKDTTTNANLSVFLVGKGYGSSDSSKLISTRLLQEHGEMDVFENMVEASTVQTSAFVLRYHHDFRYTFKDNGYIYFLFSRSPGTSDSTKITFIARLCENDHHYYSYTELQLNCTVNTEQQENTFNKVQAAYLAKPGKDLAQNIVPSNLNDKVLFGVFSADEADGRSALCMYPLSSINARFEEVIESCYTGEVLEDDKPKTVYSPYNSKNEAICKTKRDKNMVKAYICGAEFLPSPLASKPEYALAVKPIYTRNDMLTAVAVAVENEHTVAFLGTSGADVLKVHLDPNHTDFYNRIPGDRADGAVNKNLLFDSTLNHLYITTGKKISKVSVQACEQREDCHSCVSQRDPYCGWCVLEGRCTRKKDCRKGDGENAWLWSPKQTCVTIQSFEPPNISCKKTDSTQVVINIPSLPSISSPDRLECVFDGYRSQAKMEDTRVTCYLPSSDYIPPTPETQDFVAVPVKITVNDKIEVASSAFEFYNCAAVVKKSENTPCIACVTSKWGCQWNIQDHTCSDKDDSAHGSHIIGNMKANLCPQFEIPEPLRIPVGYKTPITFHGKNLDIYWGKTFQIGTELMKQVGEVTVEADGLKYRFEGYEFAYDKEPEVNVTFYIKDKDTDRKIDSTLRVVLYNCSVRREDCSLCKNAHKNDSCVWCDTTKSCIYRSLCDNELHQCPPPKITDIVPRYGPMNGRISVTIKGSNLGIKKEDIKKITVAKVPCIHQPERYSVSTSVVCEIGPFPLPPEDLPISPSKSGPVEVLVEGERTGTSEVEFTYRDPVPEAVFPVKGPAAGGTVITITGQDLDSASKDDITITVDKEPCEVLTFGHEITCKTGPYKGQKIPSDKLPVIVKYGKDTVKTVDAFEYSPDPKISEYFPQTSFVCGGRRILVIGSGFDQVQRATMRVLPSSDEFDVKASNVEFFQEVEKKNETTLEFLSPVVNKTYINLSLRTVIQLDNLVLNLTDFAYHPDPHFDKLKDKAITEGSIINVNGRGFAKAMTAKEAQAFVGDESCLVTILEDDKLFLVAPSSPPKSTSRRHRRDTSSDALELLIKFGNGEWNVGSVRFERKYEVPLYIIIPAVLIPMVLFIAVSIYCYRRKSQQAEREYEKIKNQLANLEESVRDRCKKEFTDLMIEMEDHTSDLSEARIPFLDYKNYTDRVFFLPSKDGANDVMITGKLDIPENRRAIVDQALNQFSNLLNSKTFLINFIRTLEGRQEFNARNKVYFASLLTVALHGKLEYYTDIMRTLLLELMEEYVHSKNPKLMLRRSETVVERMLCNWMSICLYQFLKDSAGEPLYKLFKAIKHQVEKGPVDAKMKKAKYTLNDTGLLGDDVEYSILTLQVLVHGEGPDVTPVKVLNCDTISQVKEKIIEQVYRNLPYSQRPVVDSVALEWRPGSTGQILSDLDLTSQKEGRWKRINTLAHYNVRDGATLVLSRVLHTQQSFDQNMDNHEERNALLEDDKVFHLVRPADELEEVKSKRGSMKDKATTKAITEIYLTRLLSMKGTLQQFVDDFFRSVLCSGTVVPPAVKYFFDFLDEQAQKHDNVDDETIHIWKTNSLPLRFWVNILKNPHFIFDVHLTEVVDASLSVIAQTFMDACTKTEHKLTRESPSNKLLYAKEISTYKKMVDDYYKGIRQMVPVSDQDMNTHLAEISRSHTDKLNTQAALHQLYQYASKYYDGIISSLEEDPAAQSKQLTLRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	XM_009300284.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio plexin b2a (plxnb2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300284.2

ORF Insert Sequence:

ATGGCGTGGA GGGCCCTGGC AGCTTTCTCT CTACTACTGT GTTTTGGCTC ATCTTGCTGT 
GATGATGCTC AAGAACAGTT CTCGTCTCCT ACCATCATCA ATAATGTGGT CCAGGACCCT 
CAAACAGGCC GAATCTACCT TGGAGCTGTC AATGGGATCT TTCAGCTGAA CCACAATCTA 
CAGGAGGAGA GCAGAGCAGA CACAGGCCCT AAGAAAGACA ACCCACAATG CACTCCACCT 
ATCACTGCGC AGTGCACCGA TGCGAAGGAA ATGGACAACT TTAACAAACT CCTTCTGGTT 
AACTCCGCCA ACGGCACCTT GATCGTTTGT GGAAGCCTTT TCAGGGGCAT CTGCTCTTTG 
GTAAACCTCA ACAGTGTGAA TAAACCGGTG TACTACAGTG ACACCAAAGG AGAGAAGACA 
TATGTTGCCA GCACTGAGGA ATCTGTCACT GTGGTGGGGG TCATTTCTTA TTTTAAGGAC 
ACTACTACTA ATGCTAACCT CAGTGTATTT CTAGTGGGAA AAGGCTATGG AAGTTCGGAC 
AGCTCAAAGC TCATTAGCAC TCGGTTGCTG CAGGAACATG GAGAAATGGA TGTTTTTGAG 
AACATGGTTG AAGCTTCCAC TGTTCAAACG AGTGCGTTTG TCCTTCGGTA CCACCACGAT 
TTTCGCTACA CGTTTAAAGA CAATGGCTAC ATCTACTTCC TGTTCTCCCG CTCTCCTGGC 
ACTAGCGACA GCACGAAGAT TACATTTATA GCACGCCTTT GCGAGAACGA CCATCATTAT 
TATTCTTACA CAGAACTTCA GCTCAACTGC ACTGTTAACA CCGAACAACA AGAGAACACA 
TTCAACAAAG TCCAAGCAGC GTATCTGGCT AAACCTGGAA AGGATTTGGC TCAGAATATC 
GTCCCGTCAA ATTTAAATGA TAAGGTTCTC TTTGGAGTGT TCAGTGCAGA TGAGGCGGAC 
GGGCGTTCAG CATTGTGCAT GTACCCGCTT AGCAGCATTA ATGCCAGGTT TGAGGAGGTG 
ATTGAGTCCT GCTACACAGG AGAAGTTCTG GAGGATGACA AACCGAAGAC TGTTTATTCA 
CCCTACAACT CCAAGAATGA AGCCATATGC AAGACCAAAA GAGATAAGAA CATGGTAAAG 
GCATATATAT GTGGCGCAGA GTTTCTGCCA TCTCCACTAG CCAGTAAACC TGAGTATGCT 
TTAGCCGTCA AACCTATCTA CACCAGGAAT GACATGCTGA CAGCTGTAGC TGTGGCGGTG 
GAGAACGAAC ACACTGTGGC ATTCTTGGGA ACCAGTGGTG CAGACGTCTT GAAGGTGCAT 
CTTGACCCAA ACCACACTGA CTTTTACAAC AGAATTCCAG GGGATCGTGC AGATGGTGCA 
GTAAACAAAA ACCTTTTGTT TGATTCAACT TTGAATCACC TGTACATCAC CACTGGCAAA 
AAGATCTCCA AAGTGTCAGT GCAGGCCTGT GAGCAGAGAG AAGATTGTCA CTCATGTGTT 
TCCCAGCGGG ACCCATATTG CGGCTGGTGT GTGTTGGAGG GCCGCTGCAC CAGGAAAAAA 
GATTGTCGAA AAGGAGATGG AGAAAACGCC TGGTTGTGGA GTCCAAAACA AACATGTGTA 
ACCATTCAGT CCTTTGAGCC CCCCAACATC AGCTGCAAGA AAACAGATAG TACACAGGTG 
GTGATCAACA TCCCGTCCCT CCCGAGCATC AGCAGTCCTG ACAGGCTGGA GTGTGTGTTT 
GACGGCTACA GGAGTCAAGC AAAGATGGAG GACACTCGGG TGACCTGCTA CCTCCCCAGC 
TCGGACTACA TCCCACCAAC ACCCGAGACA CAAGATTTTG TGGCAGTGCC AGTAAAAATC 
ACAGTGAATG ACAAGATTGA GGTGGCATCG AGTGCGTTTG AGTTTTACAA CTGTGCTGCC 
GTGGTGAAAA AATCAGAGAA TACACCATGC ATTGCATGTG TGACCAGTAA GTGGGGCTGT 
CAGTGGAATA TTCAGGATCA CACATGCAGT GATAAAGATG ACTCTGCCCA TGGGAGTCAT 
ATCATCGGAA ACATGAAGGC TAATCTGTGT CCACAGTTTG AGATTCCTGA GCCTCTGCGT 
ATTCCTGTTG GTTATAAAAC CCCCATCACA TTTCATGGCA AAAATCTGGA TATTTATTGG 
GGCAAAACCT TTCAGATCGG CACCGAGCTC ATGAAGCAGG TGGGCGAAGT GACTGTGGAG 
GCCGATGGGT TAAAGTACAG ATTTGAGGGA TATGAGTTTG CGTATGACAA AGAGCCAGAG 
GTGAATGTGA CGTTCTACAT AAAGGATAAA GACACGGATA GGAAGATTGA CAGCACCCTG 
AGAGTGGTTC TCTACAACTG TTCGGTTAGA CGCGAGGACT GCAGTCTGTG TAAAAATGCA 
CACAAGAATG ATAGCTGCGT TTGGTGCGAC ACTACAAAAT CCTGCATCTA CAGAAGCCTG 
TGTGACAATG AACTGCACCA GTGTCCTCCT CCAAAGATCA CAGACATTGT TCCCCGTTAT 
GGGCCAATGA ACGGACGCAT CTCAGTCACA ATAAAAGGCT CAAATTTGGG CATCAAGAAG 
GAGGACATTA AGAAAATCAC TGTGGCTAAA GTTCCATGCA TTCATCAGCC AGAGCGATAC 
TCGGTTTCTA CTAGTGTTGT GTGTGAGATC GGCCCTTTCC CCTTACCTCC TGAAGATCTT 
CCCATTTCAC CGAGCAAATC CGGGCCGGTG GAGGTGTTGG TGGAAGGAGA AAGAACAGGG 
ACGTCCGAGG TGGAATTCAC TTACAGGGAT CCAGTCCCAG AAGCTGTGTT CCCCGTTAAA 
GGCCCAGCAG CTGGAGGAAC TGTCATCACA ATCACAGGAC AGGACCTCGA CTCGGCCAGT 
AAAGATGACA TCACCATTAC AGTGGACAAA GAGCCATGTG AAGTGTTGAC GTTTGGCCAT 
GAGATCACCT GCAAAACTGG CCCATATAAA GGACAAAAGA TTCCCTCGGA TAAGTTGCCT 
GTTATTGTGA AGTATGGCAA GGACACTGTT AAGACAGTCG ATGCATTCGA ATACTCACCC 
GATCCCAAAA TTTCTGAATA TTTTCCACAG ACCAGCTTTG TTTGCGGTGG TCGCAGGATC 
TTAGTGATAG GCTCTGGATT TGATCAGGTT CAGAGAGCCA CCATGCGAGT GCTGCCCTCT 
TCTGACGAGT TTGACGTCAA GGCTAGCAAT GTAGAGTTCT TTCAAGAAGT GGAAAAGAAG 
AACGAGACGA CGCTGGAGTT TCTGTCGCCG GTTGTCAATA AGACATACAT CAACCTGTCC 
TTAAGAACCG TCATCCAGCT GGACAACCTG GTGCTCAATC TGACAGATTT CGCTTACCAT 
CCGGACCCAC ACTTCGATAA ACTCAAAGAT AAAGCCATAA CAGAGGGCAG CATCATCAAT 
GTTAATGGTA GAGGTTTTGC CAAAGCCATG ACTGCCAAAG AGGCACAGGC ATTTGTGGGT 
GACGAGTCGT GTCTGGTGAC AATCCTTGAG GACGATAAAC TGTTCCTGGT GGCCCCGTCC 
AGCCCTCCAA AATCCACCTC CAGACGCCAT CGAAGAGACA CCAGCTCAGA TGCCTTGGAG 
CTGCTGATCA AGTTTGGAAA TGGAGAGTGG AATGTAGGCT CCGTGCGCTT CGAGAGGAAA 
TACGAGGTTC CCCTTTACAT CATAATCCCC GCTGTTCTCA TTCCCATGGT GCTCTTCATT 
GCGGTGTCCA TCTATTGCTA CAGGAGGAAA AGCCAGCAGG CAGAACGAGA ATATGAGAAG 
ATCAAAAATC AGCTGGCCAA TTTGGAGGAG AGCGTCCGTG ATCGCTGTAA AAAGGAGTTC 
ACAGATCTAA TGATAGAGAT GGAGGATCAC ACCAGTGATC TGAGTGAAGC TCGCATCCCA 
TTCCTGGACT ACAAGAACTA CACCGACCGT GTTTTCTTCC TGCCCTCGAA AGATGGAGCC 
AATGATGTCA TGATCACCGG CAAGCTGGAT ATCCCTGAAA ACCGCAGAGC CATCGTAGAT 
CAGGCACTCA ACCAGTTCTC CAACCTCCTG AACAGCAAAA CCTTCCTTAT CAATTTTATT 
CGTACCCTGG AGGGCCGACA GGAGTTCAAT GCCAGGAATA AGGTTTACTT CGCCTCTTTG 
TTGACGGTGG CACTGCATGG CAAGTTGGAG TATTACACGG ACATCATGAG GACTCTCCTG 
CTGGAGCTCA TGGAGGAATA TGTTCACAGC AAGAATCCCA AACTCATGCT CCGCAGATCC 
GAGACTGTTG TGGAGAGGAT GTTGTGCAAC TGGATGTCGA TTTGTCTCTA CCAGTTCCTG 
AAGGACTCAG CCGGCGAGCC GCTTTATAAG CTCTTTAAGG CCATTAAACA CCAGGTGGAA 
AAAGGCCCTG TTGATGCCAA GATGAAGAAA GCCAAGTACA CTCTCAACGA CACGGGGCTT 
CTGGGAGATG ATGTTGAGTA CAGCATTTTG ACCCTACAGG TGCTGGTTCA TGGCGAGGGT 
CCGGACGTGA CCCCGGTCAA AGTGCTAAAC TGCGACACCA TCTCGCAGGT GAAGGAGAAG 
ATCATCGAGC AGGTGTACAG GAATCTGCCA TATTCCCAGA GGCCCGTGGT GGACAGCGTC 
GCACTGGAGT GGCGTCCTGG TTCTACAGGG CAGATTCTGT CTGATCTGGA CCTGACCTCA 
CAGAAGGAGG GAAGGTGGAA ACGGATCAAC ACACTGGCAC ACTATAATGT GCGAGACGGA 
GCCACCCTGG TCTTATCCAG AGTCCTGCAC ACTCAGCAGT CCTTCGACCA GAACATGGAC 
AACCATGAAG AAAGAAATGC TTTGCTGGAG GATGATAAAG TGTTTCACTT GGTGAGACCG 
GCGGATGAGC TGGAGGAGGT CAAATCTAAG AGAGGGAGCA TGAAGGACAA GGCCACCACA 
AAAGCCATCA CTGAGATCTA CCTGACCAGA CTGCTCTCCA TGAAGGGAAC GCTGCAGCAG 
TTTGTAGATG ATTTCTTCCG CAGTGTGTTG TGTTCAGGGA CTGTGGTTCC TCCAGCCGTC 
AAGTACTTCT TTGACTTCTT AGATGAACAA GCTCAAAAAC ATGACAACGT TGATGATGAG 
ACCATCCACA TATGGAAGAC CAACAGTCTT CCTCTCCGTT TCTGGGTGAA CATCTTGAAG 
AATCCTCACT TCATCTTCGA TGTTCACCTG ACTGAGGTAG TGGACGCATC TCTGTCCGTC 
ATCGCTCAGA CCTTCATGGA CGCCTGCACT AAAACCGAGC ACAAACTGAC GCGGGAGTCT 
CCCAGTAACA AACTGCTTTA TGCAAAGGAA ATCTCCACGT ACAAGAAGAT GGTTGATGAT 
TATTATAAAG GCATTCGGCA GATGGTTCCA GTCAGTGACC AAGACATGAA CACACACCTC 
GCAGAGATTT CACGGTCTCA CACAGATAAG TTGAACACCC AGGCCGCGCT GCACCAGCTC 
TACCAGTATG CCAGCAAGTA CTATGACGGG ATCATCTCGT CACTGGAGGA AGATCCTGCC 
GCCCAGAGCA AACAACTGAC CCTCAGACTG CAACAGATCG CCGCTGCGCT GGAGAATAAA 
GTCACCGACC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Novel blood collection method allows plasma proteome analysis from single zebrafish.
Journal of proteome research12(4)1580-90(2013 Apr)
Babaei F,Ramalingam R,Tavendale A,Liang Y,Yan LS,Ajuh P,Cheng SH,Lam YW

Conservation, expression, and knockdown of zebrafish plxnb2a and plxnb2b.
Developmental dynamics : an official publication of the American Association of Anatomists239(10)2722-34(2010 Oct)
Per?l? N,Peitsaro N,Sundvik M,Koivula H,Sainio K,Sariola H,Panula P,Immonen T

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PLXNB2A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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