tmem8a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmem8a
Entrez Gene ID	559126
Full Name	transmembrane protein 8A
Synonyms	si:dkeyp-75b4.6
General protein information	Preferred Names transmembrane protein 8A Names transmembrane protein 8A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001145568.1	NP_001139040.1	transmembrane protein 8A
XM_005170863.3	XP_005170920.1	transmembrane protein 8A isoform X1
XM_009305948.2	XP_009304223.1	transmembrane protein 8A isoform X2
XM_005170863.4	XP_005170920.1	transmembrane protein 8A isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TMEM8A	XP_425255.4
Gallus gallus (chicken)	LOC100858128	XP_003643243.2
Danio rerio (zebrafish)	tmem8a	NP_001139040.1
Xenopus tropicalis (tropical clawed frog)	tmem8a	XP_004918153.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Myomaker mediates fusion of fast myocytes in zebrafish embryos.
Landemaine A, Rescan PY, Gabillard JC
Biochemical and biophysical research communications451(4)480-4(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tmem8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmem8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31238	NM_001145568.1 Latest version!	Danio rerio post-glycosylphosphatidylinositol attachment to proteins 6 (pgap6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa05421	XM_005170863.4 Latest version!	Danio rerio transmembrane protein 8A (tmem8a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa42977	XM_009305948.2 Latest version!	Danio rerio transmembrane protein 8A (tmem8a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa05421	XM_005170863.3	Danio rerio transmembrane protein 8A (tmem8a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmem8a ( NM_001145568.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139040.1 Danio rerio post-glycosylphosphatidylinositol attachment to proteins 6 (pgap6), mRNA.

Price & Availability↑

CloneID	ODa31238
Clone ID Related Accession (Same CDS sequence)	NM_001145568.1
Accession Version	NM_001145568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Danio rerio(zebrafish)
Product	transmembrane protein 8A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX548256.7. On Mar 5, 2009 this sequence version replaced XM_001920332.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA4476772 [ECO:0000350] ##Evidence-Data-END##

ATGTTCGGCT CCATCACTTC AGGGTACCGG AGGATGCGGT GCTCGTGCGG TGGCTGCTCA 
CGGTCTCACG GGGATCCGGA CTCAACTGTG GCAACCAAAA CATCACTGCA CTTTAGGGCA 
GGAGCGCCCC CAGTAATTAA CCCTATTCAA ACAGCCTTTC CGAACTCTAC ATCAGCTTCT 
CTTGCTCATA ATTTGACGCT GATTGTTCCC AGTGGACAAA GTTCTAATGT GACACTGTTC 
AATGTGACGC ACCCCAAACC AGGAGACTGG TTCCTCGCTG CACACCTGCC TAAGGACGAC 
GGCAAGATTG AGCAGCAGGG ACTGCCGTCT TGCTCGTACT TATTCCAGGC GCAGATGTTT 
TCAAGAAGAG CCTCTGACCT GCCAATCCTG CAGTACAACA TTCCTCTCTC TCAAACACTT 
ACTGCATCTC CAGCTCTTTT TAAGGTTTTT GTTCCCGAAT TTTCCTCCAG TTTGGATGTT 
TTCATCTCAA ATTGTTCTGC AAATGTGGGA AATGATTGCG GGCTCTCGAT CACTGTTGGC 
TCCAGCACTT TGCGGCAGGG TTCAGAGGTG AAACAGAACT GTTCAGGCTT AAAGATCTGC 
CACGCATCTG TTCTCATTCC CCCATGGAAC ACCTGGTTAC TGGTTACCGT TGAGACCAAT 
CAACCAAACA CCTCAGTACC TTTCTACATC GCTGCCAATG TCACAGCCTT CATTCGTACC 
ATCATTGCTC CACAAAGCAA CAGCTCAGGT GTTTTCGGTA ACTCGTCTAC TTCAGCAAAG 
AACCAGTCAG GCCTTCGTTC TAACAGCTCT GAGCCAATGT GCATGAGAAG CCCGTCAGTG 
TTCAGAGATG AGCAGGACGT ACTTTCTTTT CGATACATTG TGTCCGGCAA CAACCTCACT 
GTGACCTCTG ACCTTCCAAC TGTGCTCGCA CTCGAACACT CACCTGGAGA CAGTGGAGGC 
ACACTTGTTG TACAGCTCCA GCTTAATACT AGTTCTCTGA TTGGTGGATT TGCTCGTGTG 
AAAGCATGTC TCACACCTTC TGCTCCTGTG CTTCAACTCA ACCGCAGCCA GACTTGTGCT 
ACAGGATTTG CCCAGGGTTA CACTCTGAAT GTGAGCAGCA GTGAATCAAA TGCACTCTTG 
AGGATTCCCT TCCCAGATGC AGAAAATTGG TACCTCAGCC TTCAGACCGT GTGCAATGAC 
AGTGTTGACT GCACGAATGC ATCGGCTACA GTGAGTGCAT CTGTGAGTGT TAGCGCCTGC 
ATTGATGAGT GCGGGCCGTA CGGAGAGTGC AGACTTCTGC GCACATACAA CTACCTTTAC 
GGTGCTTGTG TGTGCAAAGC AGGATGGCAG GGATGGGGTT GTACGGATGG AGTGTCTGCG 
CTCTCGTACT CTCGACAGAT GACTGCAGCT CTGCTTCTCA CCCTCAGTAA CTTTTTCTTC 
ATCCCACCCA TCATCGTAGC ACTTTACAGA GGATACCTCA TCGAGGCTGC CATCTACTTA 
TTTACCATGT TTTTTTCCAC GTTTTACCAT GCGTGTGATC AGCCCGGTGT GACTGTGATG 
TGTATCATGG ACTACGACAC TCTCCAGTTC TGTGATTTCT TGGGATCTGT CGTGTCCGTC 
TGGGTGACCA TTGTCTGCAT GGCACGGCTG CAGGAGCCAA TAAAATACCT GTTGTTTATG 
ATGGGCACTC TGGTCATTTC CATGGCGATG CAGTTGGACC GGAGGGGTCT ATGGAACCTT 
CTGGGTCCCG TCCTGTTTGC TTTAGTCATT ATGGTCACTT CTTGGGTTTA TCGAGGTGTC 
AAACGCCATC AGTGCTACCC TCCATTATGG CGCCGCTGGG TTTTGTTCCT GCTGCCAGGC 
ATCGTATCCG CTCTGATTGG AGTATGTGTG TACGTCTTCG CTCAGACCGA CAGCAACTAC 
TACTACACAC ACTCCATATG GCATGTCATG GTGGCCACAA GCGTGGTCTT CCTCCTCCCG 
CCACGAGAGA AGCACGTCCC CCCTTGGGGC TGGACCAACA AAATCTGCAG CTACAGCAGG 
TGCAAAAATG AGAAAGTGCA GCTTTACACT GTCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139040.1
CDS	85..2163
Translation	MFGSITSGYRRMRCSCGGCSRSHGDPDSTVATKTSLHFRAGAPPVINPIQTAFPNSTSASLAHNLTLIVPSGQSSNVTLFNVTHPKPGDWFLAAHLPKDDGKIEQQGLPSCSYLFQAQMFSRRASDLPILQYNIPLSQTLTASPALFKVFVPEFSSSLDVFISNCSANVGNDCGLSITVGSSTLRQGSEVKQNCSGLKICHASVLIPPWNTWLLVTVETNQPNTSVPFYIAANVTAFIRTIIAPQSNSSGVFGNSSTSAKNQSGLRSNSSEPMCMRSPSVFRDEQDVLSFRYIVSGNNLTVTSDLPTVLALEHSPGDSGGTLVVQLQLNTSSLIGGFARVKACLTPSAPVLQLNRSQTCATGFAQGYTLNVSSSESNALLRIPFPDAENWYLSLQTVCNDSVDCTNASATVSASVSVSACIDECGPYGECRLLRTYNYLYGACVCKAGWQGWGCTDGVSALSYSRQMTAALLLTLSNFFFIPPIIVALYRGYLIEAAIYLFTMFFSTFYHACDQPGVTVMCIMDYDTLQFCDFLGSVVSVWVTIVCMARLQEPIKYLLFMMGTLVISMAMQLDRRGLWNLLGPVLFALVIMVTSWVYRGVKRHQCYPPLWRRWVLFLLPGIVSALIGVCVYVFAQTDSNYYYTHSIWHVMVATSVVFLLPPREKHVPPWGWTNKICSYSRCKNEKVQLYTVT

Target ORF information:

RefSeq Version	NM_001145568.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio post-glycosylphosphatidylinositol attachment to proteins 6 (pgap6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145568.1

ORF Insert Sequence:

ATGTTCGGCT CCATCACTTC AGGGTACCGG AGGATGCGGT GCTCGTGCGG TGGCTGCTCA 
CGGTCTCACG GGGATCCGGA CTCAACTGTG GCAACCAAAA CATCACTGCA CTTTAGGGCA 
GGAGCGCCCC CAGTAATTAA CCCTATTCAA ACAGCCTTTC CGAACTCTAC ATCAGCTTCT 
CTTGCTCATA ATTTGACGCT GATTGTTCCC AGTGGACAAA GTTCTAATGT GACACTGTTC 
AATGTGACGC ACCCCAAACC AGGAGACTGG TTCCTCGCTG CACACCTGCC TAAGGACGAC 
GGCAAGATTG AGCAGCAGGG ACTGCCGTCT TGCTCGTACT TATTCCAGGC GCAGATGTTT 
TCAAGAAGAG CCTCTGACCT GCCAATCCTG CAGTACAACA TTCCTCTCTC TCAAACACTT 
ACTGCATCTC CAGCTCTTTT TAAGGTTTTT GTTCCCGAAT TTTCCTCCAG TTTGGATGTT 
TTCATCTCAA ATTGTTCTGC AAATGTGGGA AATGATTGCG GGCTCTCGAT CACTGTTGGC 
TCCAGCACTT TGCGGCAGGG TTCAGAGGTG AAACAGAACT GTTCAGGCTT AAAGATCTGC 
CACGCATCTG TTCTCATTCC CCCATGGAAC ACCTGGTTAC TGGTTACCGT TGAGACCAAT 
CAACCAAACA CCTCAGTACC TTTCTACATC GCTGCCAATG TCACAGCCTT CATTCGTACC 
ATCATTGCTC CACAAAGCAA CAGCTCAGGT GTTTTCGGTA ACTCGTCTAC TTCAGCAAAG 
AACCAGTCAG GCCTTCGTTC TAACAGCTCT GAGCCAATGT GCATGAGAAG CCCGTCAGTG 
TTCAGAGATG AGCAGGACGT ACTTTCTTTT CGATACATTG TGTCCGGCAA CAACCTCACT 
GTGACCTCTG ACCTTCCAAC TGTGCTCGCA CTCGAACACT CACCTGGAGA CAGTGGAGGC 
ACACTTGTTG TACAGCTCCA GCTTAATACT AGTTCTCTGA TTGGTGGATT TGCTCGTGTG 
AAAGCATGTC TCACACCTTC TGCTCCTGTG CTTCAACTCA ACCGCAGCCA GACTTGTGCT 
ACAGGATTTG CCCAGGGTTA CACTCTGAAT GTGAGCAGCA GTGAATCAAA TGCACTCTTG 
AGGATTCCCT TCCCAGATGC AGAAAATTGG TACCTCAGCC TTCAGACCGT GTGCAATGAC 
AGTGTTGACT GCACGAATGC ATCGGCTACA GTGAGTGCAT CTGTGAGTGT TAGCGCCTGC 
ATTGATGAGT GCGGGCCGTA CGGAGAGTGC AGACTTCTGC GCACATACAA CTACCTTTAC 
GGTGCTTGTG TGTGCAAAGC AGGATGGCAG GGATGGGGTT GTACGGATGG AGTGTCTGCG 
CTCTCGTACT CTCGACAGAT GACTGCAGCT CTGCTTCTCA CCCTCAGTAA CTTTTTCTTC 
ATCCCACCCA TCATCGTAGC ACTTTACAGA GGATACCTCA TCGAGGCTGC CATCTACTTA 
TTTACCATGT TTTTTTCCAC GTTTTACCAT GCGTGTGATC AGCCCGGTGT GACTGTGATG 
TGTATCATGG ACTACGACAC TCTCCAGTTC TGTGATTTCT TGGGATCTGT CGTGTCCGTC 
TGGGTGACCA TTGTCTGCAT GGCACGGCTG CAGGAGCCAA TAAAATACCT GTTGTTTATG 
ATGGGCACTC TGGTCATTTC CATGGCGATG CAGTTGGACC GGAGGGGTCT ATGGAACCTT 
CTGGGTCCCG TCCTGTTTGC TTTAGTCATT ATGGTCACTT CTTGGGTTTA TCGAGGTGTC 
AAACGCCATC AGTGCTACCC TCCATTATGG CGCCGCTGGG TTTTGTTCCT GCTGCCAGGC 
ATCGTATCCG CTCTGATTGG AGTATGTGTG TACGTCTTCG CTCAGACCGA CAGCAACTAC 
TACTACACAC ACTCCATATG GCATGTCATG GTGGCCACAA GCGTGGTCTT CCTCCTCCCG 
CCACGAGAGA AGCACGTCCC CCCTTGGGGC TGGACCAACA AAATCTGCAG CTACAGCAGG 
TGCAAAAATG AGAAAGTGCA GCTTTACACT GTCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem8a ( XM_005170863.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170920.1 Danio rerio transmembrane protein 8A (tmem8a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05421
Clone ID Related Accession (Same CDS sequence)	XM_005170863.3 , XM_005170863.4
Accession Version	XM_005170863.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transmembrane protein 8A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170863.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATTTTGTACT GTGCCTTTTA CTGTATGCTA CCGCTGGCTG TTTTGCTGAC 
GACCTCACTT ACTTGAACGG GTTTTACTCC AAAACGCCTC AGAGGTTGTC CAAATACAGC 
TGGTTCGGGA ATGTTCGGCT CCATCACTTC AGGGTACCGG AGGATGCGGT GCTCGTGCGG 
TGGCTGCTCA CGGTCTCACG GGGATCCGGA CTCAACTGTG GCAACCAAAA CATCACTGTG 
CACTTTAGGG CAGGAGCGCC CCCAGTAATT AACCCTATTC AAACAGCCTT TCCGAACTCT 
ACATCAGCTT CTCTTGCTCA TAATTTGACG CTGATTGTTC CCAGTGGACA AAGTTCTAAT 
GTGACACTGT TCAATGTGAC GCACCCCAAA CCAGGAGACT GGTTCCTCGC TGCACACCTG 
CCTAAGGACG ACGGCAAGAT TGAGCAGCAG GGACTGCCGT CTTGCTCGTA CTTATTCCAG 
GCGCAGATGT TTTCAAGAAG AGCCTCTGAC CTGCCAATCC TGCAGTACAA CATTCCTCTC 
TCTCAAACAC TTACTGCATC TCCAGCTCTT TTTAAGGTTT TTGTTCCCGA ATTTTCCTCC 
AGTTTGGATG TTTTCATCTC AAATTGTTCT GCAAATGTGG GAAATGATTG CGGGCTCTCG 
ATCACTGTTG GCTCCAGCAC TTTGCGGCAG GGTTCAGAGG TGAAACAGAA CTGTTCAGGC 
TTAAAGATCT GCCACGCATC TGTTCTCATT CCCCCATGGA ACACCTGGTT ACTGGTTACC 
GTTGAGACCA ATCAACCAAA CACCTCAGTA CCTTTCTACA TCGCTGCCAA TGTCACAGTT 
GGTTGTAAAC CCCTGAGCTC CTCTTCAGAT TTCAATGCTG CAGCCTTCAT TCGTACCATC 
ATTGCTCCAC AAAGCAACAG CTCAGGTGTT TTCGGTAACT CGTCTACTTC AGCAAAGAAC 
CAGTCAGGCC TTCGTTCTAA CAGCTCTGAG CCAATGTGCA TGAGAAGCCC GTCAGTGTTC 
AGAGATGAGC AGGACGTACT TTCTTTTCGA TACATTGTGT CCGGCAACAA CCTCACTGTG 
ACCTCTGACC TTCCAACTGT GCTCGCACTC GAACACTCAC CTGGAGACAG TGGAGGCACA 
CTTGTTGTAC AGCTCCAGCT TAATACTAGT TCTCTGATTG GTGGATTTGC TCGTGTGAAA 
GCATGTCTCA CACCTTCTGC TCCTGTGCTT CAACTCAACC GCAGCCAGAC TTGTGCTACA 
GGATTTGCCC AGGGTTACAC TCTGAATGTG AGCAGCAGTG AATCAAATGC ACTCTTGAGG 
ATTCCCTTCC CAGATGCAGA AAATTGGTAC CTCAGCCTTC AGACCGTGTG CAATGACAGT 
GTTGACTGCA CGAATGCATC GGCTACAGTG AGTGCATCTG TGAGTGTTAG CGCCTGCATT 
GATGAGTGCG GGCCGTACGG AGAGTGCAGA CTTCTGCGCA CATACAACTA CCTTTACGGT 
GCTTGTGTGT GCAAAGCAGG ATGGCAGGGA TGGGGTTGTA CGGATGGAGT GTCTGCGCTC 
TCGTACTCTC GACAGATGAC TGCAGCTCTG CTTCTCACCC TCAGTAACTT TTTCTTCATC 
CCACCCATCA TCGTAGCACT TTACAGAGGA TACCTCATCG AGGCTGCCAT CTACTTATTT 
ACCATGTTTT TTTCCACGTT TTACCATGCG TGTGATCAGC CCGGTGTGAC TGTGATGTGT 
ATCATGGACT ACGACACTCT CCAGTTCTGT GATTTCTTGG GATCTGTCGT GTCCGTCTGG 
GTGACCATTG TCTGCATGGC ACGGCTGCAG GAGCCAATAA AATACCTGTT GTTTATGATG 
GGCACTCTGG TCATTTCCAT GGCGATGCAG TTGGACCGGA GGGGTCTATG GAACCTTCTG 
GGTCCCGTCC TGTTTGCTTT AGTCATTATG GTCACTTCTT GGGTTTATCG AGGTGTCAAA 
CGCCATCAGT GCTACCCTCC ATTATGGCGC CGCTGGGTTT TGTTCCTGCT GCCAGGCATC 
GTATCCGCTC TGATTGGAGT ATGTGTGTAC GTCTTCGCTC AGACCGACAG CAACTACTAC 
TACACACACT CCATATGGCA TGTCATGGTG GCCACAAGCG TGGTCTTCCT CCTCCCGCCA 
CGAGAGAAGC ACGTCCCCCC TTGGGGCTGG ACCAACAAAA TCTGCAGCTA CAGCAGGTGC 
AAAAATGAGA AAGTGCAGCT TTACACTGTC ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170920.1
CDS	134..2389
Translation	MEKYFVLCLLLYATAGCFADDLTYLNGFYSKTPQRLSKYSWFGNVRLHHFRVPEDAVLVRWLLTVSRGSGLNCGNQNITVHFRAGAPPVINPIQTAFPNSTSASLAHNLTLIVPSGQSSNVTLFNVTHPKPGDWFLAAHLPKDDGKIEQQGLPSCSYLFQAQMFSRRASDLPILQYNIPLSQTLTASPALFKVFVPEFSSSLDVFISNCSANVGNDCGLSITVGSSTLRQGSEVKQNCSGLKICHASVLIPPWNTWLLVTVETNQPNTSVPFYIAANVTVGCKPLSSSSDFNAAAFIRTIIAPQSNSSGVFGNSSTSAKNQSGLRSNSSEPMCMRSPSVFRDEQDVLSFRYIVSGNNLTVTSDLPTVLALEHSPGDSGGTLVVQLQLNTSSLIGGFARVKACLTPSAPVLQLNRSQTCATGFAQGYTLNVSSSESNALLRIPFPDAENWYLSLQTVCNDSVDCTNASATVSASVSVSACIDECGPYGECRLLRTYNYLYGACVCKAGWQGWGCTDGVSALSYSRQMTAALLLTLSNFFFIPPIIVALYRGYLIEAAIYLFTMFFSTFYHACDQPGVTVMCIMDYDTLQFCDFLGSVVSVWVTIVCMARLQEPIKYLLFMMGTLVISMAMQLDRRGLWNLLGPVLFALVIMVTSWVYRGVKRHQCYPPLWRRWVLFLLPGIVSALIGVCVYVFAQTDSNYYYTHSIWHVMVATSVVFLLPPREKHVPPWGWTNKICSYSRCKNEKVQLYTVT

Target ORF information:

RefSeq Version	XM_005170863.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane protein 8A (tmem8a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170863.3

ORF Insert Sequence:

ATGGAGAAGT ATTTTGTACT GTGCCTTTTA CTGTATGCTA CCGCTGGCTG TTTTGCTGAC 
GACCTCACTT ACTTGAACGG GTTTTACTCC AAAACGCCTC AGAGGTTGTC CAAATACAGC 
TGGTTCGGGA ATGTTCGGCT CCATCACTTC AGGGTACCGG AGGATGCGGT GCTCGTGCGG 
TGGCTGCTCA CGGTCTCACG GGGATCCGGA CTCAACTGTG GCAACCAAAA CATCACTGTG 
CACTTTAGGG CAGGAGCGCC CCCAGTAATT AACCCTATTC AAACAGCCTT TCCGAACTCT 
ACATCAGCTT CTCTTGCTCA TAATTTGACG CTGATTGTTC CCAGTGGACA AAGTTCTAAT 
GTGACACTGT TCAATGTGAC GCACCCCAAA CCAGGAGACT GGTTCCTCGC TGCACACCTG 
CCTAAGGACG ACGGCAAGAT TGAGCAGCAG GGACTGCCGT CTTGCTCGTA CTTATTCCAG 
GCGCAGATGT TTTCAAGAAG AGCCTCTGAC CTGCCAATCC TGCAGTACAA CATTCCTCTC 
TCTCAAACAC TTACTGCATC TCCAGCTCTT TTTAAGGTTT TTGTTCCCGA ATTTTCCTCC 
AGTTTGGATG TTTTCATCTC AAATTGTTCT GCAAATGTGG GAAATGATTG CGGGCTCTCG 
ATCACTGTTG GCTCCAGCAC TTTGCGGCAG GGTTCAGAGG TGAAACAGAA CTGTTCAGGC 
TTAAAGATCT GCCACGCATC TGTTCTCATT CCCCCATGGA ACACCTGGTT ACTGGTTACC 
GTTGAGACCA ATCAACCAAA CACCTCAGTA CCTTTCTACA TCGCTGCCAA TGTCACAGTT 
GGTTGTAAAC CCCTGAGCTC CTCTTCAGAT TTCAATGCTG CAGCCTTCAT TCGTACCATC 
ATTGCTCCAC AAAGCAACAG CTCAGGTGTT TTCGGTAACT CGTCTACTTC AGCAAAGAAC 
CAGTCAGGCC TTCGTTCTAA CAGCTCTGAG CCAATGTGCA TGAGAAGCCC GTCAGTGTTC 
AGAGATGAGC AGGACGTACT TTCTTTTCGA TACATTGTGT CCGGCAACAA CCTCACTGTG 
ACCTCTGACC TTCCAACTGT GCTCGCACTC GAACACTCAC CTGGAGACAG TGGAGGCACA 
CTTGTTGTAC AGCTCCAGCT TAATACTAGT TCTCTGATTG GTGGATTTGC TCGTGTGAAA 
GCATGTCTCA CACCTTCTGC TCCTGTGCTT CAACTCAACC GCAGCCAGAC TTGTGCTACA 
GGATTTGCCC AGGGTTACAC TCTGAATGTG AGCAGCAGTG AATCAAATGC ACTCTTGAGG 
ATTCCCTTCC CAGATGCAGA AAATTGGTAC CTCAGCCTTC AGACCGTGTG CAATGACAGT 
GTTGACTGCA CGAATGCATC GGCTACAGTG AGTGCATCTG TGAGTGTTAG CGCCTGCATT 
GATGAGTGCG GGCCGTACGG AGAGTGCAGA CTTCTGCGCA CATACAACTA CCTTTACGGT 
GCTTGTGTGT GCAAAGCAGG ATGGCAGGGA TGGGGTTGTA CGGATGGAGT GTCTGCGCTC 
TCGTACTCTC GACAGATGAC TGCAGCTCTG CTTCTCACCC TCAGTAACTT TTTCTTCATC 
CCACCCATCA TCGTAGCACT TTACAGAGGA TACCTCATCG AGGCTGCCAT CTACTTATTT 
ACCATGTTTT TTTCCACGTT TTACCATGCG TGTGATCAGC CCGGTGTGAC TGTGATGTGT 
ATCATGGACT ACGACACTCT CCAGTTCTGT GATTTCTTGG GATCTGTCGT GTCCGTCTGG 
GTGACCATTG TCTGCATGGC ACGGCTGCAG GAGCCAATAA AATACCTGTT GTTTATGATG 
GGCACTCTGG TCATTTCCAT GGCGATGCAG TTGGACCGGA GGGGTCTATG GAACCTTCTG 
GGTCCCGTCC TGTTTGCTTT AGTCATTATG GTCACTTCTT GGGTTTATCG AGGTGTCAAA 
CGCCATCAGT GCTACCCTCC ATTATGGCGC CGCTGGGTTT TGTTCCTGCT GCCAGGCATC 
GTATCCGCTC TGATTGGAGT ATGTGTGTAC GTCTTCGCTC AGACCGACAG CAACTACTAC 
TACACACACT CCATATGGCA TGTCATGGTG GCCACAAGCG TGGTCTTCCT CCTCCCGCCA 
CGAGAGAAGC ACGTCCCCCC TTGGGGCTGG ACCAACAAAA TCTGCAGCTA CAGCAGGTGC 
AAAAATGAGA AAGTGCAGCT TTACACTGTC ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem8a ( XM_005170863.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170920.1 Danio rerio transmembrane protein 8A (tmem8a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05421
Clone ID Related Accession (Same CDS sequence)	XM_005170863.3 , XM_005170863.4
Accession Version	XM_005170863.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane protein 8A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170863.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATTTTGTACT GTGCCTTTTA CTGTATGCTA CCGCTGGCTG TTTTGCTGAC 
GACCTCACTT ACTTGAACGG GTTTTACTCC AAAACGCCTC AGAGGTTGTC CAAATACAGC 
TGGTTCGGGA ATGTTCGGCT CCATCACTTC AGGGTACCGG AGGATGCGGT GCTCGTGCGG 
TGGCTGCTCA CGGTCTCACG GGGATCCGGA CTCAACTGTG GCAACCAAAA CATCACTGTG 
CACTTTAGGG CAGGAGCGCC CCCAGTAATT AACCCTATTC AAACAGCCTT TCCGAACTCT 
ACATCAGCTT CTCTTGCTCA TAATTTGACG CTGATTGTTC CCAGTGGACA AAGTTCTAAT 
GTGACACTGT TCAATGTGAC GCACCCCAAA CCAGGAGACT GGTTCCTCGC TGCACACCTG 
CCTAAGGACG ACGGCAAGAT TGAGCAGCAG GGACTGCCGT CTTGCTCGTA CTTATTCCAG 
GCGCAGATGT TTTCAAGAAG AGCCTCTGAC CTGCCAATCC TGCAGTACAA CATTCCTCTC 
TCTCAAACAC TTACTGCATC TCCAGCTCTT TTTAAGGTTT TTGTTCCCGA ATTTTCCTCC 
AGTTTGGATG TTTTCATCTC AAATTGTTCT GCAAATGTGG GAAATGATTG CGGGCTCTCG 
ATCACTGTTG GCTCCAGCAC TTTGCGGCAG GGTTCAGAGG TGAAACAGAA CTGTTCAGGC 
TTAAAGATCT GCCACGCATC TGTTCTCATT CCCCCATGGA ACACCTGGTT ACTGGTTACC 
GTTGAGACCA ATCAACCAAA CACCTCAGTA CCTTTCTACA TCGCTGCCAA TGTCACAGTT 
GGTTGTAAAC CCCTGAGCTC CTCTTCAGAT TTCAATGCTG CAGCCTTCAT TCGTACCATC 
ATTGCTCCAC AAAGCAACAG CTCAGGTGTT TTCGGTAACT CGTCTACTTC AGCAAAGAAC 
CAGTCAGGCC TTCGTTCTAA CAGCTCTGAG CCAATGTGCA TGAGAAGCCC GTCAGTGTTC 
AGAGATGAGC AGGACGTACT TTCTTTTCGA TACATTGTGT CCGGCAACAA CCTCACTGTG 
ACCTCTGACC TTCCAACTGT GCTCGCACTC GAACACTCAC CTGGAGACAG TGGAGGCACA 
CTTGTTGTAC AGCTCCAGCT TAATACTAGT TCTCTGATTG GTGGATTTGC TCGTGTGAAA 
GCATGTCTCA CACCTTCTGC TCCTGTGCTT CAACTCAACC GCAGCCAGAC TTGTGCTACA 
GGATTTGCCC AGGGTTACAC TCTGAATGTG AGCAGCAGTG AATCAAATGC ACTCTTGAGG 
ATTCCCTTCC CAGATGCAGA AAATTGGTAC CTCAGCCTTC AGACCGTGTG CAATGACAGT 
GTTGACTGCA CGAATGCATC GGCTACAGTG AGTGCATCTG TGAGTGTTAG CGCCTGCATT 
GATGAGTGCG GGCCGTACGG AGAGTGCAGA CTTCTGCGCA CATACAACTA CCTTTACGGT 
GCTTGTGTGT GCAAAGCAGG ATGGCAGGGA TGGGGTTGTA CGGATGGAGT GTCTGCGCTC 
TCGTACTCTC GACAGATGAC TGCAGCTCTG CTTCTCACCC TCAGTAACTT TTTCTTCATC 
CCACCCATCA TCGTAGCACT TTACAGAGGA TACCTCATCG AGGCTGCCAT CTACTTATTT 
ACCATGTTTT TTTCCACGTT TTACCATGCG TGTGATCAGC CCGGTGTGAC TGTGATGTGT 
ATCATGGACT ACGACACTCT CCAGTTCTGT GATTTCTTGG GATCTGTCGT GTCCGTCTGG 
GTGACCATTG TCTGCATGGC ACGGCTGCAG GAGCCAATAA AATACCTGTT GTTTATGATG 
GGCACTCTGG TCATTTCCAT GGCGATGCAG TTGGACCGGA GGGGTCTATG GAACCTTCTG 
GGTCCCGTCC TGTTTGCTTT AGTCATTATG GTCACTTCTT GGGTTTATCG AGGTGTCAAA 
CGCCATCAGT GCTACCCTCC ATTATGGCGC CGCTGGGTTT TGTTCCTGCT GCCAGGCATC 
GTATCCGCTC TGATTGGAGT ATGTGTGTAC GTCTTCGCTC AGACCGACAG CAACTACTAC 
TACACACACT CCATATGGCA TGTCATGGTG GCCACAAGCG TGGTCTTCCT CCTCCCGCCA 
CGAGAGAAGC ACGTCCCCCC TTGGGGCTGG ACCAACAAAA TCTGCAGCTA CAGCAGGTGC 
AAAAATGAGA AAGTGCAGCT TTACACTGTC ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170920.1
CDS	136..2391
Translation	MEKYFVLCLLLYATAGCFADDLTYLNGFYSKTPQRLSKYSWFGNVRLHHFRVPEDAVLVRWLLTVSRGSGLNCGNQNITVHFRAGAPPVINPIQTAFPNSTSASLAHNLTLIVPSGQSSNVTLFNVTHPKPGDWFLAAHLPKDDGKIEQQGLPSCSYLFQAQMFSRRASDLPILQYNIPLSQTLTASPALFKVFVPEFSSSLDVFISNCSANVGNDCGLSITVGSSTLRQGSEVKQNCSGLKICHASVLIPPWNTWLLVTVETNQPNTSVPFYIAANVTVGCKPLSSSSDFNAAAFIRTIIAPQSNSSGVFGNSSTSAKNQSGLRSNSSEPMCMRSPSVFRDEQDVLSFRYIVSGNNLTVTSDLPTVLALEHSPGDSGGTLVVQLQLNTSSLIGGFARVKACLTPSAPVLQLNRSQTCATGFAQGYTLNVSSSESNALLRIPFPDAENWYLSLQTVCNDSVDCTNASATVSASVSVSACIDECGPYGECRLLRTYNYLYGACVCKAGWQGWGCTDGVSALSYSRQMTAALLLTLSNFFFIPPIIVALYRGYLIEAAIYLFTMFFSTFYHACDQPGVTVMCIMDYDTLQFCDFLGSVVSVWVTIVCMARLQEPIKYLLFMMGTLVISMAMQLDRRGLWNLLGPVLFALVIMVTSWVYRGVKRHQCYPPLWRRWVLFLLPGIVSALIGVCVYVFAQTDSNYYYTHSIWHVMVATSVVFLLPPREKHVPPWGWTNKICSYSRCKNEKVQLYTVT

Target ORF information:

RefSeq Version	XM_005170863.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane protein 8A (tmem8a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170863.4

ORF Insert Sequence:

ATGGAGAAGT ATTTTGTACT GTGCCTTTTA CTGTATGCTA CCGCTGGCTG TTTTGCTGAC 
GACCTCACTT ACTTGAACGG GTTTTACTCC AAAACGCCTC AGAGGTTGTC CAAATACAGC 
TGGTTCGGGA ATGTTCGGCT CCATCACTTC AGGGTACCGG AGGATGCGGT GCTCGTGCGG 
TGGCTGCTCA CGGTCTCACG GGGATCCGGA CTCAACTGTG GCAACCAAAA CATCACTGTG 
CACTTTAGGG CAGGAGCGCC CCCAGTAATT AACCCTATTC AAACAGCCTT TCCGAACTCT 
ACATCAGCTT CTCTTGCTCA TAATTTGACG CTGATTGTTC CCAGTGGACA AAGTTCTAAT 
GTGACACTGT TCAATGTGAC GCACCCCAAA CCAGGAGACT GGTTCCTCGC TGCACACCTG 
CCTAAGGACG ACGGCAAGAT TGAGCAGCAG GGACTGCCGT CTTGCTCGTA CTTATTCCAG 
GCGCAGATGT TTTCAAGAAG AGCCTCTGAC CTGCCAATCC TGCAGTACAA CATTCCTCTC 
TCTCAAACAC TTACTGCATC TCCAGCTCTT TTTAAGGTTT TTGTTCCCGA ATTTTCCTCC 
AGTTTGGATG TTTTCATCTC AAATTGTTCT GCAAATGTGG GAAATGATTG CGGGCTCTCG 
ATCACTGTTG GCTCCAGCAC TTTGCGGCAG GGTTCAGAGG TGAAACAGAA CTGTTCAGGC 
TTAAAGATCT GCCACGCATC TGTTCTCATT CCCCCATGGA ACACCTGGTT ACTGGTTACC 
GTTGAGACCA ATCAACCAAA CACCTCAGTA CCTTTCTACA TCGCTGCCAA TGTCACAGTT 
GGTTGTAAAC CCCTGAGCTC CTCTTCAGAT TTCAATGCTG CAGCCTTCAT TCGTACCATC 
ATTGCTCCAC AAAGCAACAG CTCAGGTGTT TTCGGTAACT CGTCTACTTC AGCAAAGAAC 
CAGTCAGGCC TTCGTTCTAA CAGCTCTGAG CCAATGTGCA TGAGAAGCCC GTCAGTGTTC 
AGAGATGAGC AGGACGTACT TTCTTTTCGA TACATTGTGT CCGGCAACAA CCTCACTGTG 
ACCTCTGACC TTCCAACTGT GCTCGCACTC GAACACTCAC CTGGAGACAG TGGAGGCACA 
CTTGTTGTAC AGCTCCAGCT TAATACTAGT TCTCTGATTG GTGGATTTGC TCGTGTGAAA 
GCATGTCTCA CACCTTCTGC TCCTGTGCTT CAACTCAACC GCAGCCAGAC TTGTGCTACA 
GGATTTGCCC AGGGTTACAC TCTGAATGTG AGCAGCAGTG AATCAAATGC ACTCTTGAGG 
ATTCCCTTCC CAGATGCAGA AAATTGGTAC CTCAGCCTTC AGACCGTGTG CAATGACAGT 
GTTGACTGCA CGAATGCATC GGCTACAGTG AGTGCATCTG TGAGTGTTAG CGCCTGCATT 
GATGAGTGCG GGCCGTACGG AGAGTGCAGA CTTCTGCGCA CATACAACTA CCTTTACGGT 
GCTTGTGTGT GCAAAGCAGG ATGGCAGGGA TGGGGTTGTA CGGATGGAGT GTCTGCGCTC 
TCGTACTCTC GACAGATGAC TGCAGCTCTG CTTCTCACCC TCAGTAACTT TTTCTTCATC 
CCACCCATCA TCGTAGCACT TTACAGAGGA TACCTCATCG AGGCTGCCAT CTACTTATTT 
ACCATGTTTT TTTCCACGTT TTACCATGCG TGTGATCAGC CCGGTGTGAC TGTGATGTGT 
ATCATGGACT ACGACACTCT CCAGTTCTGT GATTTCTTGG GATCTGTCGT GTCCGTCTGG 
GTGACCATTG TCTGCATGGC ACGGCTGCAG GAGCCAATAA AATACCTGTT GTTTATGATG 
GGCACTCTGG TCATTTCCAT GGCGATGCAG TTGGACCGGA GGGGTCTATG GAACCTTCTG 
GGTCCCGTCC TGTTTGCTTT AGTCATTATG GTCACTTCTT GGGTTTATCG AGGTGTCAAA 
CGCCATCAGT GCTACCCTCC ATTATGGCGC CGCTGGGTTT TGTTCCTGCT GCCAGGCATC 
GTATCCGCTC TGATTGGAGT ATGTGTGTAC GTCTTCGCTC AGACCGACAG CAACTACTAC 
TACACACACT CCATATGGCA TGTCATGGTG GCCACAAGCG TGGTCTTCCT CCTCCCGCCA 
CGAGAGAAGC ACGTCCCCCC TTGGGGCTGG ACCAACAAAA TCTGCAGCTA CAGCAGGTGC 
AAAAATGAGA AAGTGCAGCT TTACACTGTC ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem8a ( XM_009305948.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304223.1 Danio rerio transmembrane protein 8A (tmem8a), transcript variant X2, mRNA.

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CloneID	ODa42977
Clone ID Related Accession (Same CDS sequence)	XM_009305948.2
Accession Version	XM_009305948.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transmembrane protein 8A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305948.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGGCT CCATCACTTC AGGGTACCGG AGGATGCGGT GCTCGTGCGG TGGCTGCTCA 
CGGTCTCACG GGGATCCGGA CTCAACTGTG GCAACCAAAA CATCACTGCA CTTTAGGGCA 
GGAGCGCCCC CAGTAATTAA CCCTATTCAA ACAGCCTTTC CGAACTCTAC ATCAGCTTCT 
CTTGCTCATA ATTTGACGCT GATTGTTCCC AGTGGACAAA GTTCTAATGT GACACTGTTC 
AATGTGACGC ACCCCAAACC AGGAGACTGG TTCCTCGCTG CACACCTGCC TAAGGACGAC 
GGCAAGATTG AGCAGCAGGG ACTGCCGTCT TGCTCGTACT TATTCCAGGC GCAGATGTTT 
TCAAGAAGAG CCTCTGACCT GCCAATCCTG CAGTACAACA TTCCTCTCTC TCAAACACTT 
ACTGCATCTC CAGCTCTTTT TAAGGTTTTT GTTCCCGAAT TTTCCTCCAG TTTGGATGTT 
TTCATCTCAA ATTGTTCTGC AAATGTGGGA AATGATTGCG GGCTCTCGAT CACTGTTGGC 
TCCAGCACTT TGCGGCAGGG TTCAGAGGTG AAACAGAACT GTTCAGGCTT AAAGATCTGC 
CACGCATCTG TTCTCATTCC CCCATGGAAC ACCTGGTTAC TGGTTACCGT TGAGACCAAT 
CAACCAAACA CCTCAGTACC TTTCTACATC GCTGCCAATG TCACAGTTGG TTGTAAACCC 
CTGAGCTCCT CTTCAGATTT CAATGCTGCA GCCTTCATTC GTACCATCAT TGCTCCACAA 
AGCAACAGCT CAGGTGTTTT CGGTAACTCG TCTACTTCAG CAAAGAACCA GTCAGGCCTT 
CGTTCTAACA GCTCTGAGCC AATGTGCATG AGAAGCCCGT CAGTGTTCAG AGATGAGCAG 
GACGTACTTT CTTTTCGATA CATTGTGTCC GGCAACAACC TCACTGTGAC CTCTGACCTT 
CCAACTGTGC TCGCACTCGA ACACTCACCT GGAGACAGTG GAGGCACACT TGTTGTACAG 
CTCCAGCTTA ATACTAGTTC TCTGATTGGT GGATTTGCTC GTGTGAAAGC ATGTCTCACA 
CCTTCTGCTC CTGTGCTTCA ACTCAACCGC AGCCAGACTT GTGCTACAGG ATTTGCCCAG 
GGTTACACTC TGAATGTGAG CAGCAGTGAA TCAAATGCAC TCTTGAGGAT TCCCTTCCCA 
GATGCAGAAA ATTGGTACCT CAGCCTTCAG ACCGTGTGCA ATGACAGTGT TGACTGCACG 
AATGCATCGG CTACAGTGAG TGCATCTGTG AGTGTTAGCG CCTGCATTGA TGAGTGCGGG 
CCGTACGGAG AGTGCAGACT TCTGCGCACA TACAACTACC TTTACGGTGC TTGTGTGTGC 
AAAGCAGGAT GGCAGGGATG GGGTTGTACG GATGGAGTGT CTGCGCTCTC GTACTCTCGA 
CAGATGACTG CAGCTCTGCT TCTCACCCTC AGTAACTTTT TCTTCATCCC ACCCATCATC 
GTAGCACTTT ACAGAGGATA CCTCATCGAG GCTGCCATCT ACTTATTTAC CATGTTTTTT 
TCCACGTTTT ACCATGCGTG TGATCAGCCC GGTGTGACTG TGATGTGTAT CATGGACTAC 
GACACTCTCC AGTTCTGTGA TTTCTTGGGA TCTGTCGTGT CCGTCTGGGT GACCATTGTC 
TGCATGGCAC GGCTGCAGGA GCCAATAAAA TACCTGTTGT TTATGATGGG CACTCTGGTC 
ATTTCCATGG CGATGCAGTT GGACCGGAGG GGTCTATGGA ACCTTCTGGG TCCCGTCCTG 
TTTGCTTTAG TCATTATGGT CACTTCTTGG GTTTATCGAG GTGTCAAACG CCATCAGTGC 
TACCCTCCAT TATGGCGCCG CTGGGTTTTG TTCCTGCTGC CAGGCATCGT ATCCGCTCTG 
ATTGGAGTAT GTGTGTACGT CTTCGCTCAG ACCGACAGCA ACTACTACTA CACACACTCC 
ATATGGCATG TCATGGTGGC CACAAGCGTG GTCTTCCTCC TCCCGCCACG AGAGAAGCAC 
GTCCCCCCTT GGGGCTGGAC CAACAAAATC TGCAGCTACA GCAGGTGCAA AAATGAGAAA 
GTGCAGCTTT ACACTGTCAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304223.1
CDS	1..2124
Translation	MFGSITSGYRRMRCSCGGCSRSHGDPDSTVATKTSLHFRAGAPPVINPIQTAFPNSTSASLAHNLTLIVPSGQSSNVTLFNVTHPKPGDWFLAAHLPKDDGKIEQQGLPSCSYLFQAQMFSRRASDLPILQYNIPLSQTLTASPALFKVFVPEFSSSLDVFISNCSANVGNDCGLSITVGSSTLRQGSEVKQNCSGLKICHASVLIPPWNTWLLVTVETNQPNTSVPFYIAANVTVGCKPLSSSSDFNAAAFIRTIIAPQSNSSGVFGNSSTSAKNQSGLRSNSSEPMCMRSPSVFRDEQDVLSFRYIVSGNNLTVTSDLPTVLALEHSPGDSGGTLVVQLQLNTSSLIGGFARVKACLTPSAPVLQLNRSQTCATGFAQGYTLNVSSSESNALLRIPFPDAENWYLSLQTVCNDSVDCTNASATVSASVSVSACIDECGPYGECRLLRTYNYLYGACVCKAGWQGWGCTDGVSALSYSRQMTAALLLTLSNFFFIPPIIVALYRGYLIEAAIYLFTMFFSTFYHACDQPGVTVMCIMDYDTLQFCDFLGSVVSVWVTIVCMARLQEPIKYLLFMMGTLVISMAMQLDRRGLWNLLGPVLFALVIMVTSWVYRGVKRHQCYPPLWRRWVLFLLPGIVSALIGVCVYVFAQTDSNYYYTHSIWHVMVATSVVFLLPPREKHVPPWGWTNKICSYSRCKNEKVQLYTVT

Target ORF information:

RefSeq Version	XM_009305948.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane protein 8A (tmem8a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305948.2

ORF Insert Sequence:

ATGTTCGGCT CCATCACTTC AGGGTACCGG AGGATGCGGT GCTCGTGCGG TGGCTGCTCA 
CGGTCTCACG GGGATCCGGA CTCAACTGTG GCAACCAAAA CATCACTGCA CTTTAGGGCA 
GGAGCGCCCC CAGTAATTAA CCCTATTCAA ACAGCCTTTC CGAACTCTAC ATCAGCTTCT 
CTTGCTCATA ATTTGACGCT GATTGTTCCC AGTGGACAAA GTTCTAATGT GACACTGTTC 
AATGTGACGC ACCCCAAACC AGGAGACTGG TTCCTCGCTG CACACCTGCC TAAGGACGAC 
GGCAAGATTG AGCAGCAGGG ACTGCCGTCT TGCTCGTACT TATTCCAGGC GCAGATGTTT 
TCAAGAAGAG CCTCTGACCT GCCAATCCTG CAGTACAACA TTCCTCTCTC TCAAACACTT 
ACTGCATCTC CAGCTCTTTT TAAGGTTTTT GTTCCCGAAT TTTCCTCCAG TTTGGATGTT 
TTCATCTCAA ATTGTTCTGC AAATGTGGGA AATGATTGCG GGCTCTCGAT CACTGTTGGC 
TCCAGCACTT TGCGGCAGGG TTCAGAGGTG AAACAGAACT GTTCAGGCTT AAAGATCTGC 
CACGCATCTG TTCTCATTCC CCCATGGAAC ACCTGGTTAC TGGTTACCGT TGAGACCAAT 
CAACCAAACA CCTCAGTACC TTTCTACATC GCTGCCAATG TCACAGTTGG TTGTAAACCC 
CTGAGCTCCT CTTCAGATTT CAATGCTGCA GCCTTCATTC GTACCATCAT TGCTCCACAA 
AGCAACAGCT CAGGTGTTTT CGGTAACTCG TCTACTTCAG CAAAGAACCA GTCAGGCCTT 
CGTTCTAACA GCTCTGAGCC AATGTGCATG AGAAGCCCGT CAGTGTTCAG AGATGAGCAG 
GACGTACTTT CTTTTCGATA CATTGTGTCC GGCAACAACC TCACTGTGAC CTCTGACCTT 
CCAACTGTGC TCGCACTCGA ACACTCACCT GGAGACAGTG GAGGCACACT TGTTGTACAG 
CTCCAGCTTA ATACTAGTTC TCTGATTGGT GGATTTGCTC GTGTGAAAGC ATGTCTCACA 
CCTTCTGCTC CTGTGCTTCA ACTCAACCGC AGCCAGACTT GTGCTACAGG ATTTGCCCAG 
GGTTACACTC TGAATGTGAG CAGCAGTGAA TCAAATGCAC TCTTGAGGAT TCCCTTCCCA 
GATGCAGAAA ATTGGTACCT CAGCCTTCAG ACCGTGTGCA ATGACAGTGT TGACTGCACG 
AATGCATCGG CTACAGTGAG TGCATCTGTG AGTGTTAGCG CCTGCATTGA TGAGTGCGGG 
CCGTACGGAG AGTGCAGACT TCTGCGCACA TACAACTACC TTTACGGTGC TTGTGTGTGC 
AAAGCAGGAT GGCAGGGATG GGGTTGTACG GATGGAGTGT CTGCGCTCTC GTACTCTCGA 
CAGATGACTG CAGCTCTGCT TCTCACCCTC AGTAACTTTT TCTTCATCCC ACCCATCATC 
GTAGCACTTT ACAGAGGATA CCTCATCGAG GCTGCCATCT ACTTATTTAC CATGTTTTTT 
TCCACGTTTT ACCATGCGTG TGATCAGCCC GGTGTGACTG TGATGTGTAT CATGGACTAC 
GACACTCTCC AGTTCTGTGA TTTCTTGGGA TCTGTCGTGT CCGTCTGGGT GACCATTGTC 
TGCATGGCAC GGCTGCAGGA GCCAATAAAA TACCTGTTGT TTATGATGGG CACTCTGGTC 
ATTTCCATGG CGATGCAGTT GGACCGGAGG GGTCTATGGA ACCTTCTGGG TCCCGTCCTG 
TTTGCTTTAG TCATTATGGT CACTTCTTGG GTTTATCGAG GTGTCAAACG CCATCAGTGC 
TACCCTCCAT TATGGCGCCG CTGGGTTTTG TTCCTGCTGC CAGGCATCGT ATCCGCTCTG 
ATTGGAGTAT GTGTGTACGT CTTCGCTCAG ACCGACAGCA ACTACTACTA CACACACTCC 
ATATGGCATG TCATGGTGGC CACAAGCGTG GTCTTCCTCC TCCCGCCACG AGAGAAGCAC 
GTCCCCCCTT GGGGCTGGAC CAACAAAATC TGCAGCTACA GCAGGTGCAA AAATGAGAAA 
GTGCAGCTTT ACACTGTCAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Myomaker mediates fusion of fast myocytes in zebrafish embryos.
Biochemical and biophysical research communications451(4)480-4(2014 Sep)
Landemaine A,Rescan PY,Gabillard JC

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tmem8a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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