nphs2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nphs2
Entrez Gene ID	557914
Full Name	nephrosis 2, idiopathic, steroid-resistant (podocin)
Synonyms	nphs2l,si:dkey-171o17.6
General protein information	Preferred Names nephrosis 2, idiopathic, steroid-resistant (podocin) Names podocin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018145.2	NP_001018155.2	podocin
XM_009296250.2	XP_009294525.1	podocin isoform X1
XM_009296250.3	XP_009294525.1	podocin isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NPHS2	XP_005623019.1
Homo sapiens (human)	NPHS2	NP_055440.1
Pan troglodytes (chimpanzee)	NPHS2	XP_003308663.2
Bos taurus (cattle)	NPHS2	NP_001193036.1
Gallus gallus (chicken)	NPHS2	XP_422265.3
Mus musculus (house mouse)	Nphs2	NP_569723.1
Rattus norvegicus (Norway rat)	Nphs2	NP_570841.2
	NPHS2	XP_002802082.1
Danio rerio (zebrafish)	nphs2	NP_001018155.1
Xenopus tropicalis (tropical clawed frog)	nphs2	XP_002937013.2

Gene Ontology
Bibliography

Related articles in PubMed

Dissection of Glomerular Transcriptional Profile in Patients with Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.
Pan Y, Jiang S, Hou Q, Qiu D, Shi J, Wang L, Chen Z, Zhang M, Duan A, Qin W, Zen K, Liu Z
Diabetes(2017 Dec)

Targeting erythropoietin protects against proteinuria in type 2 diabetic patients and in zebrafish.
She J, Yuan Z, Wu Y, Chen J, Kroll J
Molecular metabolism(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lmx1b and FoxC have roles in regulating podocin expression in podocytes
Title: Lmx1b and FoxC combinatorially regulate podocin expression in podocytes.

Nephrin and podocin were specifically expressed in pronephric podocytes and required for the development of pronephric podocyte cell structure.
Title: Organization of the pronephric filtration apparatus in zebrafish requires Nephrin, Podocin and the FERM domain protein Mosaic eyes.

The following nphs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nphs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa42700	NM_001018145.2 Latest version!	Danio rerio NPHS2 stomatin family member, podocin (nphs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa42700	XM_009296250.3 Latest version!	Danio rerio nephrosis 2, idiopathic, steroid-resistant (podocin) (nphs2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa42700	XM_009296250.2	Danio rerio nephrosis 2, idiopathic, steroid-resistant (podocin) (nphs2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nphs2 ( NM_001018145.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018155.2 Danio rerio NPHS2 stomatin family member, podocin (nphs2), mRNA.

Price & Availability↑

CloneID	ODa42700
Clone ID Related Accession (Same CDS sequence)	NM_001018145.2 , XM_009296250.2 , XM_009296250.3
Accession Version	NM_001018145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	podocin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR381682.7. On Aug 21, 2015 this sequence version replaced NM_001018145.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC133976.1, AY956356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCTTCCTG CGGAGATAGA GAAAAGGACC GAAACAGAAA CATCTCGACA TTCTTCAAGA 
TCTGCCCGGA TAAAGAGAGA GCCATCGCCT TCTGCAAAAG AGAGAAAAAC CAAAGCAGCC 
AAATCTGTGA AGCTCCAAGA GCCCCACAAA AGAAAAGAGA AACTAGTAAC GACAGAAGAA 
GAAGAAGAAG CAAAAGATGC TCAGATCATC TCTAGCAGCA CGGTTGTAAA TGTAGACAGT 
GTGAGGGAAC GGATAAAAGA AGAACGAGAG GAGTTATTAG CATTATTGGA GACTGAGGGA 
CCTGGAGAAG GCCTAAAGAA GAAATACCTT GGAGTTTGTG AGCTGCTTCT CATCGTTTTG 
GTCCTGTCAG TGGTCATCCT TTTCTTACCG ATTTCAGTAT GGTTCTGTGT GAAGATTGTG 
AGAGAACACG AGAGAGCTGT CAAATTTCGC TTGGGACATC TTTTGAAGAA GAGACCCAGG 
GGCCCTGGGC TGATGTTTTA TCTCCCATTT TTGGATGTGT GTCATATAGT GGACATTCGC 
TTGCAAATAC TGAAGATTCC TCCTCATATG GTGGTGACCA AAGATCTGGT GTGTACAGAA 
GTGACTGCGG TGTGTTATTA TCGCATTGAG AACGTCTCTG TGTGTTATTC CTCCTTCGCT 
AGCATTCCAG ACGTGATGCA GGCTCTGACA CAAGTATCTG TTCGAGAGAT ACTGGCCCAT 
CATGCCTTCA ATGACATTTT GCTGGACAGG AAACGCATTG CTCAGGAGAT TCAGGTCACT 
TTAGATTCCG GAACCTGCAG GTGGGGTATT AAAGTGGAAA AAGCAGAAAT AGAAGAGATC 
AATCTTCCTC CAGAGCTGCA ACATAACTTC GCTGTAGAAG CTGAAGCAAG ACGTCAGGCA 
CAAGTTAAAG TGATCGCTGC AGAGGGAGAA AAGGCTGCAT GTGAGGCTCT GAAGGCATCT 
GTAGAGTCTG TCTCTGGCTC TCCTCTGGTC ATGCATCTTC GACTCCTCCA GCTCCTACAC 
AGTCTACGCT CTGAGCAGCC CGCCGTGGTC CTCAACATAC CACCTGATGT CCTGACCCAA 
TCCATCGACC TCGCCAGTTT GACCAGACCT GCTAATCAAA GCATGACCAC ATGTCACGGC 
TCAGATGATG GAACCAAAGA CTCACCCATG ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018155.2
CDS	40..1215
Translation	MLPAEIEKRTETETSRHSSRSARIKREPSPSAKERKTKAAKSVKLQEPHKRKEKLVTTEEEEEAKDAQIISSSTVVNVDSVRERIKEEREELLALLETEGPGEGLKKKYLGVCELLLIVLVLSVVILFLPISVWFCVKIVREHERAVKFRLGHLLKKRPRGPGLMFYLPFLDVCHIVDIRLQILKIPPHMVVTKDLVCTEVTAVCYYRIENVSVCYSSFASIPDVMQALTQVSVREILAHHAFNDILLDRKRIAQEIQVTLDSGTCRWGIKVEKAEIEEINLPPELQHNFAVEAEARRQAQVKVIAAEGEKAACEALKASVESVSGSPLVMHLRLLQLLHSLRSEQPAVVLNIPPDVLTQSIDLASLTRPANQSMTTCHGSDDGTKDSPMM

Target ORF information:

RefSeq Version	NM_001018145.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NPHS2 stomatin family member, podocin (nphs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018145.2

ORF Insert Sequence:

ATGCTTCCTG CGGAGATAGA GAAAAGGACC GAAACAGAAA CATCTCGACA TTCTTCAAGA 
TCTGCCCGGA TAAAGAGAGA GCCATCGCCT TCTGCAAAAG AGAGAAAAAC CAAAGCAGCC 
AAATCTGTGA AGCTCCAAGA GCCCCACAAA AGAAAAGAGA AACTAGTAAC GACAGAAGAA 
GAAGAAGAAG CAAAAGATGC TCAGATCATC TCTAGCAGCA CGGTTGTAAA TGTAGACAGT 
GTGAGGGAAC GGATAAAAGA AGAACGAGAG GAGTTATTAG CATTATTGGA GACTGAGGGA 
CCTGGAGAAG GCCTAAAGAA GAAATACCTT GGAGTTTGTG AGCTGCTTCT CATCGTTTTG 
GTCCTGTCAG TGGTCATCCT TTTCTTACCG ATTTCAGTAT GGTTCTGTGT GAAGATTGTG 
AGAGAACACG AGAGAGCTGT CAAATTTCGC TTGGGACATC TTTTGAAGAA GAGACCCAGG 
GGCCCTGGGC TGATGTTTTA TCTCCCATTT TTGGATGTGT GTCATATAGT GGACATTCGC 
TTGCAAATAC TGAAGATTCC TCCTCATATG GTGGTGACCA AAGATCTGGT GTGTACAGAA 
GTGACTGCGG TGTGTTATTA TCGCATTGAG AACGTCTCTG TGTGTTATTC CTCCTTCGCT 
AGCATTCCAG ACGTGATGCA GGCTCTGACA CAAGTATCTG TTCGAGAGAT ACTGGCCCAT 
CATGCCTTCA ATGACATTTT GCTGGACAGG AAACGCATTG CTCAGGAGAT TCAGGTCACT 
TTAGATTCCG GAACCTGCAG GTGGGGTATT AAAGTGGAAA AAGCAGAAAT AGAAGAGATC 
AATCTTCCTC CAGAGCTGCA ACATAACTTC GCTGTAGAAG CTGAAGCAAG ACGTCAGGCA 
CAAGTTAAAG TGATCGCTGC AGAGGGAGAA AAGGCTGCAT GTGAGGCTCT GAAGGCATCT 
GTAGAGTCTG TCTCTGGCTC TCCTCTGGTC ATGCATCTTC GACTCCTCCA GCTCCTACAC 
AGTCTACGCT CTGAGCAGCC CGCCGTGGTC CTCAACATAC CACCTGATGT CCTGACCCAA 
TCCATCGACC TCGCCAGTTT GACCAGACCT GCTAATCAAA GCATGACCAC ATGTCACGGC 
TCAGATGATG GAACCAAAGA CTCACCCATG ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nphs2 ( XM_009296250.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294525.1 Danio rerio nephrosis 2, idiopathic, steroid-resistant (podocin) (nphs2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42700
Clone ID Related Accession (Same CDS sequence)	NM_001018145.2 , XM_009296250.2 , XM_009296250.3
Accession Version	XM_009296250.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	podocin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCTG CGGAGATAGA GAAAAGGACC GAAACAGAAA CATCTCGACA TTCTTCAAGA 
TCTGCCCGGA TAAAGAGAGA GCCATCGCCT TCTGCAAAAG AGAGAAAAAC CAAAGCAGCC 
AAATCTGTGA AGCTCCAAGA GCCCCACAAA AGAAAAGAGA AACTAGTAAC GACAGAAGAA 
GAAGAAGAAG CAAAAGATGC TCAGATCATC TCTAGCAGCA CGGTTGTAAA TGTAGACAGT 
GTGAGGGAAC GGATAAAAGA AGAACGAGAG GAGTTATTAG CATTATTGGA GACTGAGGGA 
CCTGGAGAAG GCCTAAAGAA GAAATACCTT GGAGTTTGTG AGCTGCTTCT CATCGTTTTG 
GTCCTGTCAG TGGTCATCCT TTTCTTACCG ATTTCAGTAT GGTTCTGTGT GAAGATTGTG 
AGAGAACACG AGAGAGCTGT CAAATTTCGC TTGGGACATC TTTTGAAGAA GAGACCCAGG 
GGCCCTGGGC TGATGTTTTA TCTCCCATTT TTGGATGTGT GTCATATAGT GGACATTCGC 
TTGCAAATAC TGAAGATTCC TCCTCATATG GTGGTGACCA AAGATCTGGT GTGTACAGAA 
GTGACTGCGG TGTGTTATTA TCGCATTGAG AACGTCTCTG TGTGTTATTC CTCCTTCGCT 
AGCATTCCAG ACGTGATGCA GGCTCTGACA CAAGTATCTG TTCGAGAGAT ACTGGCCCAT 
CATGCCTTCA ATGACATTTT GCTGGACAGG AAACGCATTG CTCAGGAGAT TCAGGTCACT 
TTAGATTCCG GAACCTGCAG GTGGGGTATT AAAGTGGAAA AAGCAGAAAT AGAAGAGATC 
AATCTTCCTC CAGAGCTGCA ACATAACTTC GCTGTAGAAG CTGAAGCAAG ACGTCAGGCA 
CAAGTTAAAG TGATCGCTGC AGAGGGAGAA AAGGCTGCAT GTGAGGCTCT GAAGGCATCT 
GTAGAGTCTG TCTCTGGCTC TCCTCTGGTC ATGCATCTTC GACTCCTCCA GCTCCTACAC 
AGTCTACGCT CTGAGCAGCC CGCCGTGGTC CTCAACATAC CACCTGATGT CCTGACCCAA 
TCCATCGACC TCGCCAGTTT GACCAGACCT GCTAATCAAA GCATGACCAC ATGTCACGGC 
TCAGATGATG GAACCAAAGA CTCACCCATG ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294525.1
CDS	311..1486
Translation	MLPAEIEKRTETETSRHSSRSARIKREPSPSAKERKTKAAKSVKLQEPHKRKEKLVTTEEEEEAKDAQIISSSTVVNVDSVRERIKEEREELLALLETEGPGEGLKKKYLGVCELLLIVLVLSVVILFLPISVWFCVKIVREHERAVKFRLGHLLKKRPRGPGLMFYLPFLDVCHIVDIRLQILKIPPHMVVTKDLVCTEVTAVCYYRIENVSVCYSSFASIPDVMQALTQVSVREILAHHAFNDILLDRKRIAQEIQVTLDSGTCRWGIKVEKAEIEEINLPPELQHNFAVEAEARRQAQVKVIAAEGEKAACEALKASVESVSGSPLVMHLRLLQLLHSLRSEQPAVVLNIPPDVLTQSIDLASLTRPANQSMTTCHGSDDGTKDSPMM

Target ORF information:

RefSeq Version	XM_009296250.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nephrosis 2, idiopathic, steroid-resistant (podocin) (nphs2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296250.2

ORF Insert Sequence:

ATGCTTCCTG CGGAGATAGA GAAAAGGACC GAAACAGAAA CATCTCGACA TTCTTCAAGA 
TCTGCCCGGA TAAAGAGAGA GCCATCGCCT TCTGCAAAAG AGAGAAAAAC CAAAGCAGCC 
AAATCTGTGA AGCTCCAAGA GCCCCACAAA AGAAAAGAGA AACTAGTAAC GACAGAAGAA 
GAAGAAGAAG CAAAAGATGC TCAGATCATC TCTAGCAGCA CGGTTGTAAA TGTAGACAGT 
GTGAGGGAAC GGATAAAAGA AGAACGAGAG GAGTTATTAG CATTATTGGA GACTGAGGGA 
CCTGGAGAAG GCCTAAAGAA GAAATACCTT GGAGTTTGTG AGCTGCTTCT CATCGTTTTG 
GTCCTGTCAG TGGTCATCCT TTTCTTACCG ATTTCAGTAT GGTTCTGTGT GAAGATTGTG 
AGAGAACACG AGAGAGCTGT CAAATTTCGC TTGGGACATC TTTTGAAGAA GAGACCCAGG 
GGCCCTGGGC TGATGTTTTA TCTCCCATTT TTGGATGTGT GTCATATAGT GGACATTCGC 
TTGCAAATAC TGAAGATTCC TCCTCATATG GTGGTGACCA AAGATCTGGT GTGTACAGAA 
GTGACTGCGG TGTGTTATTA TCGCATTGAG AACGTCTCTG TGTGTTATTC CTCCTTCGCT 
AGCATTCCAG ACGTGATGCA GGCTCTGACA CAAGTATCTG TTCGAGAGAT ACTGGCCCAT 
CATGCCTTCA ATGACATTTT GCTGGACAGG AAACGCATTG CTCAGGAGAT TCAGGTCACT 
TTAGATTCCG GAACCTGCAG GTGGGGTATT AAAGTGGAAA AAGCAGAAAT AGAAGAGATC 
AATCTTCCTC CAGAGCTGCA ACATAACTTC GCTGTAGAAG CTGAAGCAAG ACGTCAGGCA 
CAAGTTAAAG TGATCGCTGC AGAGGGAGAA AAGGCTGCAT GTGAGGCTCT GAAGGCATCT 
GTAGAGTCTG TCTCTGGCTC TCCTCTGGTC ATGCATCTTC GACTCCTCCA GCTCCTACAC 
AGTCTACGCT CTGAGCAGCC CGCCGTGGTC CTCAACATAC CACCTGATGT CCTGACCCAA 
TCCATCGACC TCGCCAGTTT GACCAGACCT GCTAATCAAA GCATGACCAC ATGTCACGGC 
TCAGATGATG GAACCAAAGA CTCACCCATG ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nphs2 ( XM_009296250.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294525.1 Danio rerio nephrosis 2, idiopathic, steroid-resistant (podocin) (nphs2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42700
Clone ID Related Accession (Same CDS sequence)	NM_001018145.2 , XM_009296250.2 , XM_009296250.3
Accession Version	XM_009296250.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	podocin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296250.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCTG CGGAGATAGA GAAAAGGACC GAAACAGAAA CATCTCGACA TTCTTCAAGA 
TCTGCCCGGA TAAAGAGAGA GCCATCGCCT TCTGCAAAAG AGAGAAAAAC CAAAGCAGCC 
AAATCTGTGA AGCTCCAAGA GCCCCACAAA AGAAAAGAGA AACTAGTAAC GACAGAAGAA 
GAAGAAGAAG CAAAAGATGC TCAGATCATC TCTAGCAGCA CGGTTGTAAA TGTAGACAGT 
GTGAGGGAAC GGATAAAAGA AGAACGAGAG GAGTTATTAG CATTATTGGA GACTGAGGGA 
CCTGGAGAAG GCCTAAAGAA GAAATACCTT GGAGTTTGTG AGCTGCTTCT CATCGTTTTG 
GTCCTGTCAG TGGTCATCCT TTTCTTACCG ATTTCAGTAT GGTTCTGTGT GAAGATTGTG 
AGAGAACACG AGAGAGCTGT CAAATTTCGC TTGGGACATC TTTTGAAGAA GAGACCCAGG 
GGCCCTGGGC TGATGTTTTA TCTCCCATTT TTGGATGTGT GTCATATAGT GGACATTCGC 
TTGCAAATAC TGAAGATTCC TCCTCATATG GTGGTGACCA AAGATCTGGT GTGTACAGAA 
GTGACTGCGG TGTGTTATTA TCGCATTGAG AACGTCTCTG TGTGTTATTC CTCCTTCGCT 
AGCATTCCAG ACGTGATGCA GGCTCTGACA CAAGTATCTG TTCGAGAGAT ACTGGCCCAT 
CATGCCTTCA ATGACATTTT GCTGGACAGG AAACGCATTG CTCAGGAGAT TCAGGTCACT 
TTAGATTCCG GAACCTGCAG GTGGGGTATT AAAGTGGAAA AAGCAGAAAT AGAAGAGATC 
AATCTTCCTC CAGAGCTGCA ACATAACTTC GCTGTAGAAG CTGAAGCAAG ACGTCAGGCA 
CAAGTTAAAG TGATCGCTGC AGAGGGAGAA AAGGCTGCAT GTGAGGCTCT GAAGGCATCT 
GTAGAGTCTG TCTCTGGCTC TCCTCTGGTC ATGCATCTTC GACTCCTCCA GCTCCTACAC 
AGTCTACGCT CTGAGCAGCC CGCCGTGGTC CTCAACATAC CACCTGATGT CCTGACCCAA 
TCCATCGACC TCGCCAGTTT GACCAGACCT GCTAATCAAA GCATGACCAC ATGTCACGGC 
TCAGATGATG GAACCAAAGA CTCACCCATG ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294525.1
CDS	317..1492
Translation	MLPAEIEKRTETETSRHSSRSARIKREPSPSAKERKTKAAKSVKLQEPHKRKEKLVTTEEEEEAKDAQIISSSTVVNVDSVRERIKEEREELLALLETEGPGEGLKKKYLGVCELLLIVLVLSVVILFLPISVWFCVKIVREHERAVKFRLGHLLKKRPRGPGLMFYLPFLDVCHIVDIRLQILKIPPHMVVTKDLVCTEVTAVCYYRIENVSVCYSSFASIPDVMQALTQVSVREILAHHAFNDILLDRKRIAQEIQVTLDSGTCRWGIKVEKAEIEEINLPPELQHNFAVEAEARRQAQVKVIAAEGEKAACEALKASVESVSGSPLVMHLRLLQLLHSLRSEQPAVVLNIPPDVLTQSIDLASLTRPANQSMTTCHGSDDGTKDSPMM

Target ORF information:

RefSeq Version	XM_009296250.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephrosis 2, idiopathic, steroid-resistant (podocin) (nphs2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296250.3

ORF Insert Sequence:

ATGCTTCCTG CGGAGATAGA GAAAAGGACC GAAACAGAAA CATCTCGACA TTCTTCAAGA 
TCTGCCCGGA TAAAGAGAGA GCCATCGCCT TCTGCAAAAG AGAGAAAAAC CAAAGCAGCC 
AAATCTGTGA AGCTCCAAGA GCCCCACAAA AGAAAAGAGA AACTAGTAAC GACAGAAGAA 
GAAGAAGAAG CAAAAGATGC TCAGATCATC TCTAGCAGCA CGGTTGTAAA TGTAGACAGT 
GTGAGGGAAC GGATAAAAGA AGAACGAGAG GAGTTATTAG CATTATTGGA GACTGAGGGA 
CCTGGAGAAG GCCTAAAGAA GAAATACCTT GGAGTTTGTG AGCTGCTTCT CATCGTTTTG 
GTCCTGTCAG TGGTCATCCT TTTCTTACCG ATTTCAGTAT GGTTCTGTGT GAAGATTGTG 
AGAGAACACG AGAGAGCTGT CAAATTTCGC TTGGGACATC TTTTGAAGAA GAGACCCAGG 
GGCCCTGGGC TGATGTTTTA TCTCCCATTT TTGGATGTGT GTCATATAGT GGACATTCGC 
TTGCAAATAC TGAAGATTCC TCCTCATATG GTGGTGACCA AAGATCTGGT GTGTACAGAA 
GTGACTGCGG TGTGTTATTA TCGCATTGAG AACGTCTCTG TGTGTTATTC CTCCTTCGCT 
AGCATTCCAG ACGTGATGCA GGCTCTGACA CAAGTATCTG TTCGAGAGAT ACTGGCCCAT 
CATGCCTTCA ATGACATTTT GCTGGACAGG AAACGCATTG CTCAGGAGAT TCAGGTCACT 
TTAGATTCCG GAACCTGCAG GTGGGGTATT AAAGTGGAAA AAGCAGAAAT AGAAGAGATC 
AATCTTCCTC CAGAGCTGCA ACATAACTTC GCTGTAGAAG CTGAAGCAAG ACGTCAGGCA 
CAAGTTAAAG TGATCGCTGC AGAGGGAGAA AAGGCTGCAT GTGAGGCTCT GAAGGCATCT 
GTAGAGTCTG TCTCTGGCTC TCCTCTGGTC ATGCATCTTC GACTCCTCCA GCTCCTACAC 
AGTCTACGCT CTGAGCAGCC CGCCGTGGTC CTCAACATAC CACCTGATGT CCTGACCCAA 
TCCATCGACC TCGCCAGTTT GACCAGACCT GCTAATCAAA GCATGACCAC ATGTCACGGC 
TCAGATGATG GAACCAAAGA CTCACCCATG ATGTAA