sptlc2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sptlc2a
Entrez Gene ID	553646
Full Name	serine palmitoyltransferase, long chain base subunit 2a
Synonyms	sptlc2,zgc:110661
General protein information	Preferred Names serine palmitoyltransferase, long chain base subunit 2a Names serine palmitoyltransferase 2 serine palmitoyltransferase, long chain base subunit 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020619.1	NP_001018455.1	serine palmitoyltransferase 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPTLC2	XP_510095.3
Caenorhabditis elegans (roundworm)	sptl-2	NP_505065.1
Magnaporthe oryzae (rice blast fungus)	MGG_05197	XP_003712740.1
Homo sapiens (human)	SPTLC2	NP_004854.1
Rattus norvegicus (Norway rat)	Sptlc2	NP_001032174.1
Gallus gallus (chicken)	SPTLC2	NP_001006483.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007941	XP_317536.4
Schizosaccharomyces pombe (fission yeast)	lcb2	XP_001713103.1
Arabidopsis thaliana (thale cress)	LCB2	NP_197756.1
Oryza sativa (rice)	Os01g0928700	NP_001045278.1
Oryza sativa (rice)	Os01g0928800	NP_001045279.1
Mus musculus (house mouse)	Sptlc2	NP_035609.1
Kluyveromyces lactis	KLLA0D02134g	XP_453163.1
Oryza sativa (rice)	Os11g0516000	NP_001067972.1
Danio rerio (zebrafish)	sptlc2a	NP_001018455.1
Saccharomyces cerevisiae (baker's yeast)	LCB2	NP_010347.1
Neurospora crassa	NCU00447	XP_956766.1
	LOC707625	XP_001096042.2
Canis lupus familiaris (dog)	SPTLC2	XP_537524.3
Bos taurus (cattle)	SPTLC2	NP_001092551.1
Drosophila melanogaster (fruit fly)	lace	NP_476614.1
Eremothecium gossypii	AGOS_AGR127C	NP_986793.2
Arabidopsis thaliana (thale cress)	SPT1	NP_190447.1
Xenopus tropicalis (tropical clawed frog)	sptlc2	XP_002938915.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

Multiple thyrotropin ?-subunit and thyrotropin receptor-related genes arose during vertebrate evolution.
Maugars G, Dufour S, Cohen-Tannoudji J, Qu?rat B
PloS one9(11)e111361(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sptlc2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sptlc2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35036	NM_001020619.1 Latest version!	Danio rerio serine palmitoyltransferase, long chain base subunit 2a (sptlc2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sptlc2a ( NM_001020619.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018455.1 Danio rerio serine palmitoyltransferase, long chain base subunit 2a (sptlc2a), mRNA.

Price & Availability↑

CloneID	ODa35036
Clone ID Related Accession (Same CDS sequence)	NM_001020619.1
Accession Version	NM_001020619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Danio rerio(zebrafish)
Product	serine palmitoyltransferase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC095355.1. On Jul 6, 2005 this sequence version replaced XM_696799.1. ##Evidence-Data-START## Transcript exon combination :: BC095355.1, BC165370.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAAGA ACCATCATCA CCATCTTCAG TATCAAAAGC AGGAGAAGGT TTACCATGCT 
GCATGCAATG GAGAACTGTA CAGCAGGCCG TTCATCGAGT CCTTCGAGGA AACTCCCATG 
CTGGTTGCCG TGCTCACGTA CATGGGCTAC GGCATCCTTA CGATCTTTGG ATATCTGCGG 
GATTTTATGA GGGAGTGGAA AATAGAAAAA TGCCACATCG CCAGAGAAAG GGAGGAGCAG 
AAGGACTTTG TTCCACTTTA TCAAGACTTT GAGAATTTCT ACACCAGGAA TTTATACATG 
AGGATTCGGG ACAGCTGGAA CAGGCCCATT TGCAGTGTTC CCGGGGCCAA GTTTGACCTT 
GTTGAGCGCA GTTCCAATGA TTACAACTGG ACCTTTGAGT ACACTGGGCG AGTGGTGAAG 
GATGTGATCA ACCTGGGATC GTATAATTAC CTGGGCTTTG CAGAGAACAC TGGCTCATGT 
GCTACAGCTG CTGGTGAGGT GACTGTGAAA TTTGGGGTGG GCGTGGCCGG CACCAGGCAG 
GAAATGGGCA ACCTCGACAG ACACGAGAAG TTGGAGAAAC TGGTGGCGTC ATTTCTGAGA 
GTGGAGTCTG CTATGGCGTT CGGAATGGGA TTTGCAACTA ACTCCATGAA CATTCCTGCA 
TTAGCTGGCA AGGGATGTCT GATTCTGAGT GATGAGTTAA ACCATGCCTC TCTAGTACTG 
GGAGCTCGTC TTTCAGGCTC CACCATCCGG GTCTTTAAGC ACAATAATAT GCAGAGCCTG 
GAGAAATTAC TCAGAGACGC AATCGTTCAT GGCCAACCTC GAACACATCG ACCGTGGAAG 
AAAATCCTTA TTCTTGTAGA GGGAATCTAC AGTATGGAGG GCTCCATAAT TCGGCTGCCA 
GAAGTTATAG CTCTGAAAAA GAAGTACAAA GCCTACCTTT ACCTGGACGA AGCCCACAGT 
ATTGGCGCTT TGGGACCCGG CGGTCGAGGG GTGGTGGAGT ATTTCGGACT CGACCCCAGA 
GATGTAGACA TCATGATGGG CACTTTCACA AAGAGCTTTG GGGCTGCTGG TGGATACATA 
GGAGGGAGGA AGGATTTGAT AGACTACTTG CGAACTCACT CCCACAGTGC TGTTTATGCG 
TCCTCCATGT CTGCTCCTGT TGTGGAGCAA ATCATCACTT CTATGAAGTG CATTATGGGA 
GAGGATGGAA CGACAATAGG TCGTTCACGT ATCCAGCGGT TGGCTGAAAA CACAGTCTAT 
TTCCGTAGGA GACTGCGGGA GATGGGATTC ATCATCTATG GCAACAATGA CTCACCTGTG 
GTACCCATGA TGCTCTACAT GCCCGCCAAA ATCGGGGCAT TTGGTCGGGA GATGTTGAAG 
AGAAACATTG GTGTAGTGGT GGTGGGTTTT CCAGCCACGC CGATCATCGA GTCCAGGGCC 
CGTTTCTGTA TCTCTGCGGC CCATTCAAAG GAAATGCTGG ACCGGGCACT GGATGTGATC 
AGTGAAGTAG GAGATCTGCT GCAGTTGAAG TATTCGCGGC ACAAAGTCCT TCCCTCCCTG 
GATCGACCCT TTGACGAAAC CACATATGAA GAAAACGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018455.1
CDS	165..1769
Translation	MVKNHHHHLQYQKQEKVYHAACNGELYSRPFIESFEETPMLVAVLTYMGYGILTIFGYLRDFMREWKIEKCHIAREREEQKDFVPLYQDFENFYTRNLYMRIRDSWNRPICSVPGAKFDLVERSSNDYNWTFEYTGRVVKDVINLGSYNYLGFAENTGSCATAAGEVTVKFGVGVAGTRQEMGNLDRHEKLEKLVASFLRVESAMAFGMGFATNSMNIPALAGKGCLILSDELNHASLVLGARLSGSTIRVFKHNNMQSLEKLLRDAIVHGQPRTHRPWKKILILVEGIYSMEGSIIRLPEVIALKKKYKAYLYLDEAHSIGALGPGGRGVVEYFGLDPRDVDIMMGTFTKSFGAAGGYIGGRKDLIDYLRTHSHSAVYASSMSAPVVEQIITSMKCIMGEDGTTIGRSRIQRLAENTVYFRRRLREMGFIIYGNNDSPVVPMMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCISAAHSKEMLDRALDVISEVGDLLQLKYSRHKVLPSLDRPFDETTYEENEE

Target ORF information:

RefSeq Version	NM_001020619.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio serine palmitoyltransferase, long chain base subunit 2a (sptlc2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020619.1

ORF Insert Sequence:

ATGGTGAAGA ACCATCATCA CCATCTTCAG TATCAAAAGC AGGAGAAGGT TTACCATGCT 
GCATGCAATG GAGAACTGTA CAGCAGGCCG TTCATCGAGT CCTTCGAGGA AACTCCCATG 
CTGGTTGCCG TGCTCACGTA CATGGGCTAC GGCATCCTTA CGATCTTTGG ATATCTGCGG 
GATTTTATGA GGGAGTGGAA AATAGAAAAA TGCCACATCG CCAGAGAAAG GGAGGAGCAG 
AAGGACTTTG TTCCACTTTA TCAAGACTTT GAGAATTTCT ACACCAGGAA TTTATACATG 
AGGATTCGGG ACAGCTGGAA CAGGCCCATT TGCAGTGTTC CCGGGGCCAA GTTTGACCTT 
GTTGAGCGCA GTTCCAATGA TTACAACTGG ACCTTTGAGT ACACTGGGCG AGTGGTGAAG 
GATGTGATCA ACCTGGGATC GTATAATTAC CTGGGCTTTG CAGAGAACAC TGGCTCATGT 
GCTACAGCTG CTGGTGAGGT GACTGTGAAA TTTGGGGTGG GCGTGGCCGG CACCAGGCAG 
GAAATGGGCA ACCTCGACAG ACACGAGAAG TTGGAGAAAC TGGTGGCGTC ATTTCTGAGA 
GTGGAGTCTG CTATGGCGTT CGGAATGGGA TTTGCAACTA ACTCCATGAA CATTCCTGCA 
TTAGCTGGCA AGGGATGTCT GATTCTGAGT GATGAGTTAA ACCATGCCTC TCTAGTACTG 
GGAGCTCGTC TTTCAGGCTC CACCATCCGG GTCTTTAAGC ACAATAATAT GCAGAGCCTG 
GAGAAATTAC TCAGAGACGC AATCGTTCAT GGCCAACCTC GAACACATCG ACCGTGGAAG 
AAAATCCTTA TTCTTGTAGA GGGAATCTAC AGTATGGAGG GCTCCATAAT TCGGCTGCCA 
GAAGTTATAG CTCTGAAAAA GAAGTACAAA GCCTACCTTT ACCTGGACGA AGCCCACAGT 
ATTGGCGCTT TGGGACCCGG CGGTCGAGGG GTGGTGGAGT ATTTCGGACT CGACCCCAGA 
GATGTAGACA TCATGATGGG CACTTTCACA AAGAGCTTTG GGGCTGCTGG TGGATACATA 
GGAGGGAGGA AGGATTTGAT AGACTACTTG CGAACTCACT CCCACAGTGC TGTTTATGCG 
TCCTCCATGT CTGCTCCTGT TGTGGAGCAA ATCATCACTT CTATGAAGTG CATTATGGGA 
GAGGATGGAA CGACAATAGG TCGTTCACGT ATCCAGCGGT TGGCTGAAAA CACAGTCTAT 
TTCCGTAGGA GACTGCGGGA GATGGGATTC ATCATCTATG GCAACAATGA CTCACCTGTG 
GTACCCATGA TGCTCTACAT GCCCGCCAAA ATCGGGGCAT TTGGTCGGGA GATGTTGAAG 
AGAAACATTG GTGTAGTGGT GGTGGGTTTT CCAGCCACGC CGATCATCGA GTCCAGGGCC 
CGTTTCTGTA TCTCTGCGGC CCATTCAAAG GAAATGCTGG ACCGGGCACT GGATGTGATC 
AGTGAAGTAG GAGATCTGCT GCAGTTGAAG TATTCGCGGC ACAAAGTCCT TCCCTCCCTG 
GATCGACCCT TTGACGAAAC CACATATGAA GAAAACGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

Multiple thyrotropin ?-subunit and thyrotropin receptor-related genes arose during vertebrate evolution.
PloS one9(11)e111361(2014)
Maugars G,Dufour S,Cohen-Tannoudji J,Qu?rat B