mpeg1.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mpeg1.2
Entrez Gene ID	553612
Full Name	macrophage expressed 1, tandem duplicate 2
Synonyms	zgc:110354
General protein information	Preferred Names macrophage expressed 1, tandem duplicate 2 Names uncharacterized protein LOC553612 macrophage expressed gene 1, tandem duplicate 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020586.2	NP_001018422.2	uncharacterized protein LOC553612 precursor
NM_001020586.1	NP_001018422.1	uncharacterized protein LOC553612 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MPEG1	XP_508450.1
Mus musculus (house mouse)	Mpeg1	NP_034951.1
Canis lupus familiaris (dog)	MPEG1	XP_533169.1
Homo sapiens (human)	MPEG1	NP_001034485.1
Rattus norvegicus (Norway rat)	Mpeg1	NP_072139.1
Danio rerio (zebrafish)	zgc:110354	NP_001018422.1
Danio rerio (zebrafish)	si:ch211-247l22.3	NP_001116782.1
Gallus gallus (chicken)	MPEG1	XP_003641422.1
Danio rerio (zebrafish)	mpeg1	NP_997902.1
Bos taurus (cattle)	MPEG1	NP_001039929.1

Gene Ontology
Bibliography

Related articles in PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
Tang Q, Iyer S, Lobbardi R, Moore JC, Chen H, Lareau C, Hebert C, Shaw ML, Neftel C, Suva ML, Ceol CJ, Bernards A, Aryee M, Pinello L, Drummond IA, Langenau DM
The Journal of experimental medicine214(10)2875-2887(2017 Oct)

A novel perivascular cell population in the zebrafish brain.
Venero Galanternik M, Castranova D, Gore AV, Blewett NH, Jung HM, Stratman AN, Kirby MR, Iben J, Miller MF, Kawakami K, Maraia RJ, Weinstein BM
eLife6(2017 04)

Further characterisation of differences between TL and AB zebrafish (Danio rerio): Gene expression, physiology and behaviour at day 5 of the larval stage.
van den Bos R, Mes W, Galligani P, Heil A, Zethof J, Flik G, Gorissen M
PloS one12(4)e0175420(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mpeg1.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mpeg1.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02585	NM_001020586.1 Latest version!	Danio rerio macrophage expressed 1, tandem duplicate 2 (mpeg1.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa159826	NM_001020586.2 Latest version!	Danio rerio macrophage expressed 1, tandem duplicate 2 (mpeg1.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mpeg1.2 ( NM_001020586.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018422.1 Danio rerio macrophage expressed 1, tandem duplicate 2 (mpeg1.2), mRNA.

Price & Availability↑

CloneID	ODa02585
Clone ID Related Accession (Same CDS sequence)	NM_001020586.1
Accession Version	NM_001020586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC553612 precursor
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC095229.1. ##Evidence-Data-START## Transcript exon combination :: BC095229.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAATTTAT TATATATTTT CCTTACATTC TTCGTCCTTT TCTGCAAATC TGATGGCTTT 
ATCAGACCCA GTAATGGTCT CCGCGAATGC CGTCAAAACT CAAATTTAAC AGCTCTAGAG 
GTGCTCCCAG GTGGAGGTTG GGACAACCTG CGCAATTTAG ACGTGGGACG GGTGATGAAC 
TTGACCTATT CCCAGTGTCA GACCACAGAA GATGGACTTT ATCTCATCCC AGACGAAGTG 
TTTGTCATTC CCCGAAAAAC AAGCGGAGTG GAAACTCACT CTGAGACTAT AATGTCATGG 
CTGGATCAGA GAAGCTCCAC ATCAGGATCC ATCAATGCAG ACGCTTCCTA CCTTACTGTT 
CTAAACGGAA AATTCTCTGA GGAAAACACG CGCATGAAAA TACACCAAGT GAGAGGGAAT 
TCCGTAACAA CACGAGTTCA GGTACGCAAC CATCTGTACA CCGTGAATGC TTACCCTGAC 
TTCACCCTGG ATTCCCGCTT TTCCCAGCAG ATCTCGGAAC TCGCAGATGC TATTGAGAAC 
AATCAAACAC GTCAAGCAAT GTATTTGTCA GAGAAAGTCA TACTTGAATA TGGTACCCAT 
GTCATCACAA GCATCGATGC CGGTGCCACT TTAGTGCAGG AGGACTATAT AAAAAGGTCT 
TACGTCTCTG ACACTAATTC AGAGAGGTCT TCTGTCTCTG CATCCGCAGG CATTAACTTT 
TTTAACAAGG TTAATTTTAA CTTTGGCAGC AAAGAAACCG AGCAAACCTC TGAAACCCTC 
ACTTACCAGC AAAACATCAC ATACTCATTA GTTCAGAGCC ACGGCGGGGC TTTATTCTAT 
CAAGGCATCA CTATGCAGAA GTGGCAAGAG AGCACTCAAA ATAATCTTGT GGCGATTGAC 
CGTTCTGGTC TGCCGATTCA CTATTTTCTC AATCCAGCCG TATTCCCTGA CCTCCCAGTT 
CCCACTTTAC ATAAGCTTGC CTTTTCAGTG CAGAAGGCTG CAGAACGTTA CTACAACATA 
AACACCACTC CAGGCTGCAC AGATCAAAGT TCTCAAAATT TTAACTTTCA GGCCAATGTG 
AACGACACGT CCTGTCAGGG TCCAGTCACC AATTTGACAT TTGGTGGCGT TTACCAAACA 
TGCACTCCTG AGTCACCAGA TGGAAATGTC ATCTGTGATG AATTTGCACA AAGAAATCCA 
GACACTGGTG ACTTTACTTG CCGTCAACCA TACAGTGCCA CTCGGTTACA TTCTGAGAAC 
GTACAGCAGG GTTATAATAA TTATGAGTGC CATGAGGAAT GTCATTCATG CTGGTTAATT 
TTGGATTGTT GTCATCAAGT ATGTGGAAAT GTTTACTACG TCCGCCAGGC ACAAATTGAC 
ACTTATTGGT GTTCCACAAG TCAGACAGTC CCTGAAAACA CCGGATACCT ATTTGGGGGT 
CTATTTGGAC CATCTTTACA AAACCCACAG ACCAAATCTC ATGACTGCCC TGCAAATTAT 
TTTGAACAAG TATTTCTGAC TAACGGCATG AGGATTTGTC TGAGCAATGA CTATGAGGCA 
GGAACAATAT CCTCTGTGCC ATTTGGGGGT TTATTTAGCT GCCAATCTGG CAACCCTCAA 
GCAGGAAACC AGTACCGTTG TCCACCTCAG TACAGCCAAC ACCTTGCAAC TATTAGCGAT 
GGCTGTCAAG TATTGTACTG TGTACAATCG GGTGTGTTTA CTGGCGGCCA GTTAAAACCT 
GTTCACCTCC CACCTTTCAC AAAGCCACCT CTGATCAACA TGATGGCGAC AAACACAGTC 
GCTGTAATGA CAGAAGGCGA TAAAGCCTGG ATGAGGGTTG GGGGGACAAA AAAATGGAGA 
GTGGCAAACC TTGGAGAAGT CAGTCGAATG GCCAAACTGA TCGATGCATC ATCCACCCAG 
ATGTCAGGAG GGGAAAAGGC CGGTGTTGCT TGCGGTGTGA TGACTTTGGT CGCTATTGTG 
GTGGCAGGAA TTGTGATCTT AATGAAACGG AGGAGAAAGT TTTCTCATTT CAGTTTTAAA 
AGGGGATATG AAGAGATTCA GCACAATGGC CAGAGCACTG TAGAGATTGA AAGAGAGCAG 
CAGAATACAA ATGAAAACCC CACTCAACCT CTGCTACCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018422.1
CDS	67..2208
Translation	MNLLYIFLTFFVLFCKSDGFIRPSNGLRECRQNSNLTALEVLPGGGWDNLRNLDVGRVMNLTYSQCQTTEDGLYLIPDEVFVIPRKTSGVETHSETIMSWLDQRSSTSGSINADASYLTVLNGKFSEENTRMKIHQVRGNSVTTRVQVRNHLYTVNAYPDFTLDSRFSQQISELADAIENNQTRQAMYLSEKVILEYGTHVITSIDAGATLVQEDYIKRSYVSDTNSERSSVSASAGINFFNKVNFNFGSKETEQTSETLTYQQNITYSLVQSHGGALFYQGITMQKWQESTQNNLVAIDRSGLPIHYFLNPAVFPDLPVPTLHKLAFSVQKAAERYYNINTTPGCTDQSSQNFNFQANVNDTSCQGPVTNLTFGGVYQTCTPESPDGNVICDEFAQRNPDTGDFTCRQPYSATRLHSENVQQGYNNYECHEECHSCWLILDCCHQVCGNVYYVRQAQIDTYWCSTSQTVPENTGYLFGGLFGPSLQNPQTKSHDCPANYFEQVFLTNGMRICLSNDYEAGTISSVPFGGLFSCQSGNPQAGNQYRCPPQYSQHLATISDGCQVLYCVQSGVFTGGQLKPVHLPPFTKPPLINMMATNTVAVMTEGDKAWMRVGGTKKWRVANLGEVSRMAKLIDASSTQMSGGEKAGVACGVMTLVAIVVAGIVILMKRRRKFSHFSFKRGYEEIQHNGQSTVEIEREQQNTNENPTQPLLP

Target ORF information:

RefSeq Version	NM_001020586.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio macrophage expressed 1, tandem duplicate 2 (mpeg1.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020586.1

ORF Insert Sequence:

ATGAATTTAT TATATATTTT CCTTACATTC TTCGTCCTTT TCTGCAAATC TGATGGCTTT 
ATCAGACCCA GTAATGGTCT CCGCGAATGC CGTCAAAACT CAAATTTAAC AGCTCTAGAG 
GTGCTCCCAG GTGGAGGTTG GGACAACCTG CGCAATTTAG ACGTGGGACG GGTGATGAAC 
TTGACCTATT CCCAGTGTCA GACCACAGAA GATGGACTTT ATCTCATCCC AGACGAAGTG 
TTTGTCATTC CCCGAAAAAC AAGCGGAGTG GAAACTCACT CTGAGACTAT AATGTCATGG 
CTGGATCAGA GAAGCTCCAC ATCAGGATCC ATCAATGCAG ACGCTTCCTA CCTTACTGTT 
CTAAACGGAA AATTCTCTGA GGAAAACACG CGCATGAAAA TACACCAAGT GAGAGGGAAT 
TCCGTAACAA CACGAGTTCA GGTACGCAAC CATCTGTACA CCGTGAATGC TTACCCTGAC 
TTCACCCTGG ATTCCCGCTT TTCCCAGCAG ATCTCGGAAC TCGCAGATGC TATTGAGAAC 
AATCAAACAC GTCAAGCAAT GTATTTGTCA GAGAAAGTCA TACTTGAATA TGGTACCCAT 
GTCATCACAA GCATCGATGC CGGTGCCACT TTAGTGCAGG AGGACTATAT AAAAAGGTCT 
TACGTCTCTG ACACTAATTC AGAGAGGTCT TCTGTCTCTG CATCCGCAGG CATTAACTTT 
TTTAACAAGG TTAATTTTAA CTTTGGCAGC AAAGAAACCG AGCAAACCTC TGAAACCCTC 
ACTTACCAGC AAAACATCAC ATACTCATTA GTTCAGAGCC ACGGCGGGGC TTTATTCTAT 
CAAGGCATCA CTATGCAGAA GTGGCAAGAG AGCACTCAAA ATAATCTTGT GGCGATTGAC 
CGTTCTGGTC TGCCGATTCA CTATTTTCTC AATCCAGCCG TATTCCCTGA CCTCCCAGTT 
CCCACTTTAC ATAAGCTTGC CTTTTCAGTG CAGAAGGCTG CAGAACGTTA CTACAACATA 
AACACCACTC CAGGCTGCAC AGATCAAAGT TCTCAAAATT TTAACTTTCA GGCCAATGTG 
AACGACACGT CCTGTCAGGG TCCAGTCACC AATTTGACAT TTGGTGGCGT TTACCAAACA 
TGCACTCCTG AGTCACCAGA TGGAAATGTC ATCTGTGATG AATTTGCACA AAGAAATCCA 
GACACTGGTG ACTTTACTTG CCGTCAACCA TACAGTGCCA CTCGGTTACA TTCTGAGAAC 
GTACAGCAGG GTTATAATAA TTATGAGTGC CATGAGGAAT GTCATTCATG CTGGTTAATT 
TTGGATTGTT GTCATCAAGT ATGTGGAAAT GTTTACTACG TCCGCCAGGC ACAAATTGAC 
ACTTATTGGT GTTCCACAAG TCAGACAGTC CCTGAAAACA CCGGATACCT ATTTGGGGGT 
CTATTTGGAC CATCTTTACA AAACCCACAG ACCAAATCTC ATGACTGCCC TGCAAATTAT 
TTTGAACAAG TATTTCTGAC TAACGGCATG AGGATTTGTC TGAGCAATGA CTATGAGGCA 
GGAACAATAT CCTCTGTGCC ATTTGGGGGT TTATTTAGCT GCCAATCTGG CAACCCTCAA 
GCAGGAAACC AGTACCGTTG TCCACCTCAG TACAGCCAAC ACCTTGCAAC TATTAGCGAT 
GGCTGTCAAG TATTGTACTG TGTACAATCG GGTGTGTTTA CTGGCGGCCA GTTAAAACCT 
GTTCACCTCC CACCTTTCAC AAAGCCACCT CTGATCAACA TGATGGCGAC AAACACAGTC 
GCTGTAATGA CAGAAGGCGA TAAAGCCTGG ATGAGGGTTG GGGGGACAAA AAAATGGAGA 
GTGGCAAACC TTGGAGAAGT CAGTCGAATG GCCAAACTGA TCGATGCATC ATCCACCCAG 
ATGTCAGGAG GGGAAAAGGC CGGTGTTGCT TGCGGTGTGA TGACTTTGGT CGCTATTGTG 
GTGGCAGGAA TTGTGATCTT AATGAAACGG AGGAGAAAGT TTTCTCATTT CAGTTTTAAA 
AGGGGATATG AAGAGATTCA GCACAATGGC CAGAGCACTG TAGAGATTGA AAGAGAGCAG 
CAGAATACAA ATGAAAACCC CACTCAACCT CTGCTACCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mpeg1.2 ( NM_001020586.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018422.2 Danio rerio macrophage expressed 1, tandem duplicate 2 (mpeg1.2), mRNA.

Price & Availability↑

CloneID	ODa159826
Clone ID Related Accession (Same CDS sequence)	NM_001020586.2
Accession Version	NM_001020586.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC553612 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU984571.8. On Apr 20, 2018 this sequence version replaced NM_001020586.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC095229.1, GFIL01044373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTTAT TATATATTTT CCTTACATTC TTCGTCCTTT TCTGCAAATC TGATGGCTTT 
ATCAGACCCA GTAATGGTCT CCGCGAATGC CGCCAAAACT CAAATTTAAC AGCTCTAGAG 
GTGCTCCCAG GTGGAGGTTG GGACAACCTG CGCAATTTAG ACGTGGGACG GGTGATGAAC 
TTGACCTATT CCCAGTGTCA GACCACAGAA GATGGACTTT ATCTCATCCC AGACGAAGTG 
TTTGTCATTC CGCGAAAAAC AAGCGGAGTG GAAACTCACT CTGAGACTAT AATGTCATGG 
CTGGATCAGA GAAGCTCCAC GTCAGGATCC ATCAATGCAG ACGCTTCCTA CCTTACTGTT 
CTAAACGGAA AATTCTCTGA GGAAAACACG CGCATGAAAA TACACCAAGT GAGAGGGAAT 
TCCGTAACAA CACGAGTTCA GGTACGCAAC CATCTGTACA CCGTGAATGC TTACCCTGAC 
TTCACCCTGG ATTCCCGCTT TTCCCAGCAG ATCTCGGAAC TCGCAGATGC TATTGAGAAC 
AATCAAACAC GTCAAGCAAT GTATTTGTCA GAGAAAGTCA TACTTGAATA TGGTACCCAT 
GTCATCACAA GCATCGATGC CGGTGCCACT TTAGTGCAGG AGGACTATAT AAAAAGGTCT 
TACGTCTCTG ACACTAATTC AGAGAGGTCT TCTGTCTCTG CATCCGCAGG CATTAACTTT 
TTTAACATGG TTAATTTTAA CTTTGGCAGC AAAGAAACCG AGCAAACCTC TGAAACCCTC 
ACTTACCAGC AAAACATCAC ATACTCATTA GTTCAGAGCC ACGGCGGGGC TTTATTCTAT 
CAAGGCATCA CTATGCAGAA GTGGCAAGAG AGCACTCAAA ATCATCTTGT GGCAATTGAC 
CGTTCTGGTC TGCCGATTCA CTATTTTCTC AATCCAGCCG TATTCCCTGA CCTCCCAGTT 
CCCACTTTAC ATAAGCTTGC CTTTTCAGTG CAGAAGGCTG CAGAACGTTA CTACAACATA 
AACACCACTC CAGGCTGCAC AGATCAAAGT TCTCAAAACT TTAACTTTCA GGCCAATGTG 
AACGACACGT CCTGTGAGGG TCCAGTCACC AATTTGACAT TTGGTGGCGT TTACCAAACA 
TGCACTCCTG AGTCACCAGA TGGAAATGTC ATCTGTGATG AATTTGCACA AAGAAATCCA 
GACACTGGTG ACTTTACTTG CCGTCAACCA TACAGTGCCA CTCGGTTACA TTCTGAGAAC 
GTACAGCAGG GTTATAATAA TTATGAGTGC CATGAGGAAT GTCATTCATG CTGGTTAATT 
TTGGATTGTT GTCATCAAGT ATGTGGAAAT GTTTACTACG TCCGCCAGGC ACAAATTGAC 
ACTTATTGGT GTTCCACAAG TCAGACAGTC CCTGAAAACA CCGGATACCT ATTTGGGGGT 
CTATTTGGAC CATCTTTACA AAACCCACAG ACCAAATCTC ATGACTGCCC TGCAAATTAT 
TTTGAACAAG TATTTCTGAC TAACGGCATG AGGATTTGTC TGAGCAATGA CTATGAGGCA 
GGAACAATAT CCTCTGTGCC ATTTGGGGGT TTATTTAGCT GCCAATCTGG CAACCCTCAA 
GCAGGAAACC AGTACCGTTG TCCACCTCAG TACAGCCAAC ACCTTGCAAC TATTAGCGAT 
GGCTGTCAAG TATTGTACTG TGTACAATCG GGTGTGTTTA CTGGCGGCCA GTTAAAACCT 
GTTCACCTCC CACCTTTCAC AAAGCCACCT CTGATCAACA TGATGGCGAC AAACACAGTC 
GCTGTAATGA CAGAAGGCGA TAAAGCCTGG ATGAGGGTTG GGGGGACAAA AAAATGGAGA 
GTGGCAAACC TTGGAGAAGT CAGTCGAATG GCCAAACTGA TCGATGCATC ATCCACCCAG 
ATGTCAGGAG GGGAAAAGGC CGGTGTTGCT TGCGGTGTGA TGACTTTGGT CGCTATTGTG 
GTGGCAGGAA TTGTGATCTT AATGAAACGG AGGAGAAAGT TTTCTCATTT CAGTTTTAAA 
AGGGGATATG AAGAGATTCA GCATGATGGC CAGAGCACTG TAGAGATTGA AAGAGAGCAG 
CAGAATACAA ATGAAAACCC CACTCAACCT CTGCTACCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018422.2
CDS	66..2207
Translation	MNLLYIFLTFFVLFCKSDGFIRPSNGLRECRQNSNLTALEVLPGGGWDNLRNLDVGRVMNLTYSQCQTTEDGLYLIPDEVFVIPRKTSGVETHSETIMSWLDQRSSTSGSINADASYLTVLNGKFSEENTRMKIHQVRGNSVTTRVQVRNHLYTVNAYPDFTLDSRFSQQISELADAIENNQTRQAMYLSEKVILEYGTHVITSIDAGATLVQEDYIKRSYVSDTNSERSSVSASAGINFFNMVNFNFGSKETEQTSETLTYQQNITYSLVQSHGGALFYQGITMQKWQESTQNHLVAIDRSGLPIHYFLNPAVFPDLPVPTLHKLAFSVQKAAERYYNINTTPGCTDQSSQNFNFQANVNDTSCEGPVTNLTFGGVYQTCTPESPDGNVICDEFAQRNPDTGDFTCRQPYSATRLHSENVQQGYNNYECHEECHSCWLILDCCHQVCGNVYYVRQAQIDTYWCSTSQTVPENTGYLFGGLFGPSLQNPQTKSHDCPANYFEQVFLTNGMRICLSNDYEAGTISSVPFGGLFSCQSGNPQAGNQYRCPPQYSQHLATISDGCQVLYCVQSGVFTGGQLKPVHLPPFTKPPLINMMATNTVAVMTEGDKAWMRVGGTKKWRVANLGEVSRMAKLIDASSTQMSGGEKAGVACGVMTLVAIVVAGIVILMKRRRKFSHFSFKRGYEEIQHDGQSTVEIEREQQNTNENPTQPLLP

Target ORF information:

RefSeq Version	NM_001020586.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio macrophage expressed 1, tandem duplicate 2 (mpeg1.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020586.2

ORF Insert Sequence:

ATGAATTTAT TATATATTTT CCTTACATTC TTCGTCCTTT TCTGCAAATC TGATGGCTTT 
ATCAGACCCA GTAATGGTCT CCGCGAATGC CGCCAAAACT CAAATTTAAC AGCTCTAGAG 
GTGCTCCCAG GTGGAGGTTG GGACAACCTG CGCAATTTAG ACGTGGGACG GGTGATGAAC 
TTGACCTATT CCCAGTGTCA GACCACAGAA GATGGACTTT ATCTCATCCC AGACGAAGTG 
TTTGTCATTC CGCGAAAAAC AAGCGGAGTG GAAACTCACT CTGAGACTAT AATGTCATGG 
CTGGATCAGA GAAGCTCCAC GTCAGGATCC ATCAATGCAG ACGCTTCCTA CCTTACTGTT 
CTAAACGGAA AATTCTCTGA GGAAAACACG CGCATGAAAA TACACCAAGT GAGAGGGAAT 
TCCGTAACAA CACGAGTTCA GGTACGCAAC CATCTGTACA CCGTGAATGC TTACCCTGAC 
TTCACCCTGG ATTCCCGCTT TTCCCAGCAG ATCTCGGAAC TCGCAGATGC TATTGAGAAC 
AATCAAACAC GTCAAGCAAT GTATTTGTCA GAGAAAGTCA TACTTGAATA TGGTACCCAT 
GTCATCACAA GCATCGATGC CGGTGCCACT TTAGTGCAGG AGGACTATAT AAAAAGGTCT 
TACGTCTCTG ACACTAATTC AGAGAGGTCT TCTGTCTCTG CATCCGCAGG CATTAACTTT 
TTTAACATGG TTAATTTTAA CTTTGGCAGC AAAGAAACCG AGCAAACCTC TGAAACCCTC 
ACTTACCAGC AAAACATCAC ATACTCATTA GTTCAGAGCC ACGGCGGGGC TTTATTCTAT 
CAAGGCATCA CTATGCAGAA GTGGCAAGAG AGCACTCAAA ATCATCTTGT GGCAATTGAC 
CGTTCTGGTC TGCCGATTCA CTATTTTCTC AATCCAGCCG TATTCCCTGA CCTCCCAGTT 
CCCACTTTAC ATAAGCTTGC CTTTTCAGTG CAGAAGGCTG CAGAACGTTA CTACAACATA 
AACACCACTC CAGGCTGCAC AGATCAAAGT TCTCAAAACT TTAACTTTCA GGCCAATGTG 
AACGACACGT CCTGTGAGGG TCCAGTCACC AATTTGACAT TTGGTGGCGT TTACCAAACA 
TGCACTCCTG AGTCACCAGA TGGAAATGTC ATCTGTGATG AATTTGCACA AAGAAATCCA 
GACACTGGTG ACTTTACTTG CCGTCAACCA TACAGTGCCA CTCGGTTACA TTCTGAGAAC 
GTACAGCAGG GTTATAATAA TTATGAGTGC CATGAGGAAT GTCATTCATG CTGGTTAATT 
TTGGATTGTT GTCATCAAGT ATGTGGAAAT GTTTACTACG TCCGCCAGGC ACAAATTGAC 
ACTTATTGGT GTTCCACAAG TCAGACAGTC CCTGAAAACA CCGGATACCT ATTTGGGGGT 
CTATTTGGAC CATCTTTACA AAACCCACAG ACCAAATCTC ATGACTGCCC TGCAAATTAT 
TTTGAACAAG TATTTCTGAC TAACGGCATG AGGATTTGTC TGAGCAATGA CTATGAGGCA 
GGAACAATAT CCTCTGTGCC ATTTGGGGGT TTATTTAGCT GCCAATCTGG CAACCCTCAA 
GCAGGAAACC AGTACCGTTG TCCACCTCAG TACAGCCAAC ACCTTGCAAC TATTAGCGAT 
GGCTGTCAAG TATTGTACTG TGTACAATCG GGTGTGTTTA CTGGCGGCCA GTTAAAACCT 
GTTCACCTCC CACCTTTCAC AAAGCCACCT CTGATCAACA TGATGGCGAC AAACACAGTC 
GCTGTAATGA CAGAAGGCGA TAAAGCCTGG ATGAGGGTTG GGGGGACAAA AAAATGGAGA 
GTGGCAAACC TTGGAGAAGT CAGTCGAATG GCCAAACTGA TCGATGCATC ATCCACCCAG 
ATGTCAGGAG GGGAAAAGGC CGGTGTTGCT TGCGGTGTGA TGACTTTGGT CGCTATTGTG 
GTGGCAGGAA TTGTGATCTT AATGAAACGG AGGAGAAAGT TTTCTCATTT CAGTTTTAAA 
AGGGGATATG AAGAGATTCA GCATGATGGC CAGAGCACTG TAGAGATTGA AAGAGAGCAG 
CAGAATACAA ATGAAAACCC CACTCAACCT CTGCTACCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
The Journal of experimental medicine214(10)2875-2887(2017 Oct)
Tang Q,Iyer S,Lobbardi R,Moore JC,Chen H,Lareau C,Hebert C,Shaw ML,Neftel C,Suva ML,Ceol CJ,Bernards A,Aryee M,Pinello L,Drummond IA,Langenau DM

A novel perivascular cell population in the zebrafish brain.
eLife6(2017 04)
Venero Galanternik M,Castranova D,Gore AV,Blewett NH,Jung HM,Stratman AN,Kirby MR,Iben J,Miller MF,Kawakami K,Maraia RJ,Weinstein BM

Further characterisation of differences between TL and AB zebrafish (Danio rerio): Gene expression, physiology and behaviour at day 5 of the larval stage.
PloS one12(4)e0175420(2017)
van den Bos R,Mes W,Galligani P,Heil A,Zethof J,Flik G,Gorissen M