prrg4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prrg4
Entrez Gene ID	550330
Full Name	proline rich Gla (G-carboxyglutamic acid) 4 (transmembrane)
Synonyms	zgc:110234
General protein information	Preferred Names proline rich Gla (G-carboxyglutamic acid) 4 (transmembrane) Names transmembrane gamma-carboxyglutamic acid protein 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017637.2	NP_001017637.1	transmembrane gamma-carboxyglutamic acid protein 4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRRG4	NP_076986.1
Pan troglodytes (chimpanzee)	PRRG4	XP_521881.2
Bos taurus (cattle)	PRRG4	NP_001180054.1
Mus musculus (house mouse)	Prrg4	NP_848810.3
Rattus norvegicus (Norway rat)	Prrg4	NP_001102673.1
Xenopus tropicalis (tropical clawed frog)	LOC100486092	XP_002938759.2
Canis lupus familiaris (dog)	PRRG4	XP_851497.2
Gallus gallus (chicken)	PRRG4	NP_001186344.1
Danio rerio (zebrafish)	prrg4	NP_001017637.1
	PRRG4	XP_001084029.2

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evolution of the vertebrate Pax4/6 class of genes with focus on its novel member, the Pax10 gene.
Feiner N, Meyer A, Kuraku S
Genome biology and evolution6(7)1635-51(2014 Jun)

Regulatory back-up circuit of medaka Wt1 co-orthologs ensures PGC maintenance.
Kl?ver N, Herpin A, Braasch I, Driessle J, Schartl M
Developmental biology325(1)179-88(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prrg4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prrg4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa23574	NM_001017637.2 Latest version!	Danio rerio proline rich Gla (G-carboxyglutamic acid) 4 (transmembrane) (prrg4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prrg4 ( NM_001017637.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017637.1 Danio rerio proline rich Gla (G-carboxyglutamic acid) 4 (transmembrane) (prrg4), mRNA.

Price & Availability↑

CloneID	ODa23574
Clone ID Related Accession (Same CDS sequence)	NM_001017637.2
Accession Version	NM_001017637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	transmembrane gamma-carboxyglutamic acid protein 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH538457.1 and CU896647.6. On or before Feb 17, 2016 this sequence version replaced XM_005169966.2, NM_001017637.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01012982.1, BC092813.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCTTATAT TTGTCCTCCT GCTGCTGTGT CATTTCACGT TATGTGGACA CTGTGCGTGT 
ACGAGGAAAA CCCTTCAGAT AGGAGCAGAA CAAGAGAGTG AAGTGTTTGT TGTGGACCAG 
GAGGCCAACA CATTTTTAGG ACGCCACCTG TTGTTCAACC GCTTTGACTT TGAGATCTTT 
ACCCCGGGTA ATCTCGAACG GGAGTGTATC GAGGAGATAT GCAATTACGA GGAGGCTCGG 
GAAGTCTTCG AGGATATCCC CAAAACAAAT GACTTTTGGA GGACATATAC AGAGGCTAAG 
GGTGAGTCCC AGTCAAAAGT TGACGTAATA GTTTTGCTGG TGGGCATCAT CTCCGCTGGA 
GTCTTTATCA TTATTATTGG CCTCCTCATC TGGTATTTCT GCCAGGGCAG GAATAAAAAC 
GATGGTTTCC GGGGCTCCTT CAGACGTCGA TCCACCCGAA GCAATGCCTC GGTAATCATC 
CGCAGACTGG AAGAGGTTTC TCTGCATCCT ATTCCTCCCG CAGAGGCCAG TGCAGGTTTA 
CCTTCATACG AACAGGCTAT TGCCATCACT GGACCACATG ATGCTCCGCC ACCACCATAT 
CCTGGTTCCA GGCCAGGAAG TATGAGACGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017637.1
CDS	170..802
Translation	MLIFVLLLLCHFTLCGHCACTRKTLQIGAEQESEVFVVDQEANTFLGRHLLFNRFDFEIFTPGNLERECIEEICNYEEAREVFEDIPKTNDFWRTYTEAKGESQSKVDVIVLLVGIISAGVFIIIIGLLIWYFCQGRNKNDGFRGSFRRRSTRSNASVIIRRLEEVSLHPIPPAEASAGLPSYEQAIAITGPHDAPPPPYPGSRPGSMRR

Target ORF information:

RefSeq Version	NM_001017637.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proline rich Gla (G-carboxyglutamic acid) 4 (transmembrane) (prrg4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017637.2

ORF Insert Sequence:

ATGCTTATAT TTGTCCTCCT GCTGCTGTGT CATTTCACGT TATGTGGACA CTGTGCGTGT 
ACGAGGAAAA CCCTTCAGAT AGGAGCAGAA CAAGAGAGTG AAGTGTTTGT TGTGGACCAG 
GAGGCCAACA CATTTTTAGG ACGCCACCTG TTGTTCAACC GCTTTGACTT TGAGATCTTT 
ACCCCGGGTA ATCTCGAACG GGAGTGTATC GAGGAGATAT GCAATTACGA GGAGGCTCGG 
GAAGTCTTCG AGGATATCCC CAAAACAAAT GACTTTTGGA GGACATATAC AGAGGCTAAG 
GGTGAGTCCC AGTCAAAAGT TGACGTAATA GTTTTGCTGG TGGGCATCAT CTCCGCTGGA 
GTCTTTATCA TTATTATTGG CCTCCTCATC TGGTATTTCT GCCAGGGCAG GAATAAAAAC 
GATGGTTTCC GGGGCTCCTT CAGACGTCGA TCCACCCGAA GCAATGCCTC GGTAATCATC 
CGCAGACTGG AAGAGGTTTC TCTGCATCCT ATTCCTCCCG CAGAGGCCAG TGCAGGTTTA 
CCTTCATACG AACAGGCTAT TGCCATCACT GGACCACATG ATGCTCCGCC ACCACCATAT 
CCTGGTTCCA GGCCAGGAAG TATGAGACGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evolution of the vertebrate Pax4/6 class of genes with focus on its novel member, the Pax10 gene.
Genome biology and evolution6(7)1635-51(2014 Jun)
Feiner N,Meyer A,Kuraku S

Regulatory back-up circuit of medaka Wt1 co-orthologs ensures PGC maintenance.
Developmental biology325(1)179-88(2009 Jan)
Kl?ver N,Herpin A,Braasch I,Driessle J,Schartl M