ISLR2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ISLR2
Entrez Gene ID	541396
Full Name	immunoglobulin superfamily containing leucine-rich repeat 2
Synonyms	zgc:101901
General protein information	Preferred Names immunoglobulin superfamily containing leucine-rich repeat 2 Names immunoglobulin superfamily containing leucine-rich repeat protein 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013523.1	NP_001013541.1	immunoglobulin superfamily containing leucine-rich repeat protein 2 precursor
XM_009298031.2	XP_009296306.1	immunoglobulin superfamily containing leucine-rich repeat protein 2 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ISLR2	XP_005222076.1
Mus musculus (house mouse)	Islr2	NP_001155007.1
Gallus gallus (chicken)	ISLR2	NP_001038132.1
Homo sapiens (human)	ISLR2	NP_001123609.1
	ISLR2	XP_001096799.2
Pan troglodytes (chimpanzee)	ISLR2	XP_003314716.2
Canis lupus familiaris (dog)	ISLR2	XP_005638649.1
Danio rerio (zebrafish)	islr2	NP_001013541.1
Rattus norvegicus (Norway rat)	LOC100910729	XP_003750566.1
Xenopus tropicalis (tropical clawed frog)	islr2	NP_001120371.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The LRR receptor Islr2 is required for retinal axon routing at the vertebrate optic chiasm.
Panza P, Sitko AA, Maischein HM, Koch I, Fl?tenmeyer M, Wright GJ, Mandai K, Mason CA, S?llner C
Neural development1023(2015 Oct)

A Floor-Plate Extracellular Protein-Protein Interaction Screen Identifies Draxin as a Secreted Netrin-1 Antagonist.
Gao X, Metzger U, Panza P, Mahalwar P, Alsheimer S, Geiger H, Maischein HM, Levesque MP, Templin M, S?llner C
Cell reports12(4)694-708(2015 Jul)

Profiling, Bioinformatic, and Functional Data on the Developing Olfactory/GnRH System Reveal Cellular and Molecular Pathways Essential for This Process and Potentially Relevant for the Kallmann Syndrome.
Garaffo G, Provero P, Molineris I, Pinciroli P, Peano C, Battaglia C, Tomaiuolo D, Etzion T, Gothilf Y, Santoro M, Merlo GR
Frontiers in endocrinology4203(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we identify a novel role for the LRR receptor Islr2 in ensuring proper axon navigation at the optic chiasm of both zebrafish and mouse.
Title: The LRR receptor Islr2 is required for retinal axon routing at the vertebrate optic chiasm.

The following ISLR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISLR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01190	NM_001013523.1 Latest version!	Danio rerio immunoglobulin superfamily containing leucine-rich repeat 2 (islr2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa41618	XM_009298031.2 Latest version!	Danio rerio immunoglobulin superfamily containing leucine-rich repeat 2 (islr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ISLR2 ( NM_001013523.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001013541.1 Danio rerio immunoglobulin superfamily containing leucine-rich repeat 2 (islr2), mRNA.

Price & Availability↑

CloneID	ODa01190
Clone ID Related Accession (Same CDS sequence)	NM_001013523.1
Accession Version	NM_001013523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	immunoglobulin superfamily containing leucine-rich repeat protein 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC090831.1. ##Evidence-Data-START## Transcript exon combination :: BC090831.1, EH435890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAACCA AATACCTGAT GCTTATTGCC CTGGGGACTG CATTGATTGG CAATGCACAC 
TGTTGCCCCA AGCAGTGTGC CTGCTCTGAT AAATACAACC ACCAGTTCGT AGACTGTGCC 
TACAAAGACC TTGTGGAAGT ACCTGTGGGT TTGCCGTCCA ATGCAAGCAC CCTGAGTCTT 
TCAGCGAACA AGATTAAAGT GCTGAAATCC AAAACTTTTG TAAACGTGAC CCAGGTGACC 
TCTCTTTGGC TGGCTCACAA TGAAATCATC ACAGTCGAGA GGGACACGTT GGCTCCATTG 
ATTCAGCTGA AAAATTTGGA TATTAGCAAC AACAAAATTG TGCATTTTCC ATGGGAGGAC 
TTGGCCAATC TTGGTGCTTT GCAGTTGTTA AAGATGAACA ATAATGAGAT GGTCAGCATC 
CCTAAGAACG CCTTTTCAAA CTTGAAAGAC CTGCGTTCAA TCCGTATTAA CAATAACAAG 
TTTACCACTA TTGTGCAAGG AACATTTGAT TCTTTGGCTG CCATGTCCCA TCTGCAAATC 
TTCCACAACC CCTTCATCTG CTCCTGCAAC CTTGAGTGGC TTAGGGACTG GATCCTCAAA 
TCCTCAATCT CCATTCCTGA ACAAAACAAC ATCGCTTGCG ATGCCCCATC CCACCTCAAA 
GGTTCTCAGG TCACTAGCAT GCCCAAATTG AATTGCAAGG CCCCATCTGT ATCCATTACA 
TATCAGCCAA ACATAGAAAA GACTGAACTC TATGAGGGAT ATATGGTTAT GCTATACTGC 
GAAACAAAAG GAACACCCAA ACCCGAGGTC ACATGGGAGA TATTTGCTGG AAACCAACTG 
ATTACATTCC CTTTGCCTGC TATTGTTGAA AAAAGTGACA TTCCGATTAA TGGCCCACCT 
ACAAACACCA GGTTCCTCGT GTTTCAAAAT GGCACCCTGA TCGTTCCTCG CATGAGCAAG 
AAAGAAGAGG GAAACTACAC CTGTTCTGCT GTTAATGACA TAGGAAAAGC TGAGAGCTCA 
GTTAGACTTG TTGTCGCAGG CACTAAAAAG CACGCCACCA ATTCTATGCT GGACACGAAA 
TCACCAGGGC TTTTACCAGA AGACAAACTC GGTTCAAAAG GCTCCAAAAA CAGCGTGATT 
AGCATGAGGC CTAAATTTGA AGAGAAGACT AAAAGTCTTC CCACTGGCAC ATCTGTCATT 
ACAGTGGATA AAGAGCAAGT AGATGAAGGG GCTGATACTT TGCCATTTGT TGGTAAATGT 
GGAATAAATG ACGGCACACA GTACATTTCT AACCATGCTT TCAACCTCAG CTTGGATGAA 
CTTAAACAGT ACACATTTGA TTTTGGGGTT ATCGCTCTGG AAGTTTCAGA GACTGAGGCT 
AAAGTTCAGC TGAACCCTAT GCAGATGGCC AACGCAAAGT CAAACATTCA CCTCAGTCAA 
CAACAAGACC TCCAAACTGT GAATAAGGAG CCTTTTAGTC TTTTCCAAAC ATCCGCCAAA 
AAGTCACCAC TAGACTTGTT GTACCTTTGT GTCAGTACTG GCAATGGACA TTCAGTGGTG 
CAGTGGTCCA AGATAGAAGA AGGCGTCAAC GCCTATCGCT TTCAGCACCT CAAGCCAGGC 
ACCAATTACA CCCTCTGTCT CACCTACGGA GGCCAAGACT GCCAAGTCCA AGTGGTCTTC 
ACAACCCGAA AGAAAATCCC ATCCTTGTTA ATCATTGTAG TCGTTAGCAT CTTTCTACTA 
GCTTTGGCAA CCGTGCCCCT TCTGGGTGCC ACCTGCTGTC ATTTGCTCTA CAAGTATCAA 
GGCAAGACCT ACAAGCTGAT TATGAAAACA CAAAACCCAG ATCAGATGGA AAAGAACATA 
GCGACAGATT TTGATCCCAG GGCGTCATTT GTGGGATCCG AAAAGAATTT TAACCCCAGT 
GAGATGGGAG AGGGAGAAGG CGAGGCTGAT GGCGAGGAGG GAGACGGAGA AGGAGAGGAC 
ATCGAGGGCA GTGTGGTGAC CGAGTCCATT CCAGAGTCAC AGTCCAAAAC ACAAGAGGAG 
TTTGAAGTCG GTTCCGAGTA CAGCGATCGG TTACCCCTTG GAGCTGAAGC AGTAAACATA 
TCAGCGGAGA TCAACGGTAA TTACAAACAA CCACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013541.1
CDS	241..2379
Translation	MATKYLMLIALGTALIGNAHCCPKQCACSDKYNHQFVDCAYKDLVEVPVGLPSNASTLSLSANKIKVLKSKTFVNVTQVTSLWLAHNEIITVERDTLAPLIQLKNLDISNNKIVHFPWEDLANLGALQLLKMNNNEMVSIPKNAFSNLKDLRSIRINNNKFTTIVQGTFDSLAAMSHLQIFHNPFICSCNLEWLRDWILKSSISIPEQNNIACDAPSHLKGSQVTSMPKLNCKAPSVSITYQPNIEKTELYEGYMVMLYCETKGTPKPEVTWEIFAGNQLITFPLPAIVEKSDIPINGPPTNTRFLVFQNGTLIVPRMSKKEEGNYTCSAVNDIGKAESSVRLVVAGTKKHATNSMLDTKSPGLLPEDKLGSKGSKNSVISMRPKFEEKTKSLPTGTSVITVDKEQVDEGADTLPFVGKCGINDGTQYISNHAFNLSLDELKQYTFDFGVIALEVSETEAKVQLNPMQMANAKSNIHLSQQQDLQTVNKEPFSLFQTSAKKSPLDLLYLCVSTGNGHSVVQWSKIEEGVNAYRFQHLKPGTNYTLCLTYGGQDCQVQVVFTTRKKIPSLLIIVVVSIFLLALATVPLLGATCCHLLYKYQGKTYKLIMKTQNPDQMEKNIATDFDPRASFVGSEKNFNPSEMGEGEGEADGEEGDGEGEDIEGSVVTESIPESQSKTQEEFEVGSEYSDRLPLGAEAVNISAEINGNYKQPR

Target ORF information:

RefSeq Version	NM_001013523.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio immunoglobulin superfamily containing leucine-rich repeat 2 (islr2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013523.1

ORF Insert Sequence:

ATGGCAACCA AATACCTGAT GCTTATTGCC CTGGGGACTG CATTGATTGG CAATGCACAC 
TGTTGCCCCA AGCAGTGTGC CTGCTCTGAT AAATACAACC ACCAGTTCGT AGACTGTGCC 
TACAAAGACC TTGTGGAAGT ACCTGTGGGT TTGCCGTCCA ATGCAAGCAC CCTGAGTCTT 
TCAGCGAACA AGATTAAAGT GCTGAAATCC AAAACTTTTG TAAACGTGAC CCAGGTGACC 
TCTCTTTGGC TGGCTCACAA TGAAATCATC ACAGTCGAGA GGGACACGTT GGCTCCATTG 
ATTCAGCTGA AAAATTTGGA TATTAGCAAC AACAAAATTG TGCATTTTCC ATGGGAGGAC 
TTGGCCAATC TTGGTGCTTT GCAGTTGTTA AAGATGAACA ATAATGAGAT GGTCAGCATC 
CCTAAGAACG CCTTTTCAAA CTTGAAAGAC CTGCGTTCAA TCCGTATTAA CAATAACAAG 
TTTACCACTA TTGTGCAAGG AACATTTGAT TCTTTGGCTG CCATGTCCCA TCTGCAAATC 
TTCCACAACC CCTTCATCTG CTCCTGCAAC CTTGAGTGGC TTAGGGACTG GATCCTCAAA 
TCCTCAATCT CCATTCCTGA ACAAAACAAC ATCGCTTGCG ATGCCCCATC CCACCTCAAA 
GGTTCTCAGG TCACTAGCAT GCCCAAATTG AATTGCAAGG CCCCATCTGT ATCCATTACA 
TATCAGCCAA ACATAGAAAA GACTGAACTC TATGAGGGAT ATATGGTTAT GCTATACTGC 
GAAACAAAAG GAACACCCAA ACCCGAGGTC ACATGGGAGA TATTTGCTGG AAACCAACTG 
ATTACATTCC CTTTGCCTGC TATTGTTGAA AAAAGTGACA TTCCGATTAA TGGCCCACCT 
ACAAACACCA GGTTCCTCGT GTTTCAAAAT GGCACCCTGA TCGTTCCTCG CATGAGCAAG 
AAAGAAGAGG GAAACTACAC CTGTTCTGCT GTTAATGACA TAGGAAAAGC TGAGAGCTCA 
GTTAGACTTG TTGTCGCAGG CACTAAAAAG CACGCCACCA ATTCTATGCT GGACACGAAA 
TCACCAGGGC TTTTACCAGA AGACAAACTC GGTTCAAAAG GCTCCAAAAA CAGCGTGATT 
AGCATGAGGC CTAAATTTGA AGAGAAGACT AAAAGTCTTC CCACTGGCAC ATCTGTCATT 
ACAGTGGATA AAGAGCAAGT AGATGAAGGG GCTGATACTT TGCCATTTGT TGGTAAATGT 
GGAATAAATG ACGGCACACA GTACATTTCT AACCATGCTT TCAACCTCAG CTTGGATGAA 
CTTAAACAGT ACACATTTGA TTTTGGGGTT ATCGCTCTGG AAGTTTCAGA GACTGAGGCT 
AAAGTTCAGC TGAACCCTAT GCAGATGGCC AACGCAAAGT CAAACATTCA CCTCAGTCAA 
CAACAAGACC TCCAAACTGT GAATAAGGAG CCTTTTAGTC TTTTCCAAAC ATCCGCCAAA 
AAGTCACCAC TAGACTTGTT GTACCTTTGT GTCAGTACTG GCAATGGACA TTCAGTGGTG 
CAGTGGTCCA AGATAGAAGA AGGCGTCAAC GCCTATCGCT TTCAGCACCT CAAGCCAGGC 
ACCAATTACA CCCTCTGTCT CACCTACGGA GGCCAAGACT GCCAAGTCCA AGTGGTCTTC 
ACAACCCGAA AGAAAATCCC ATCCTTGTTA ATCATTGTAG TCGTTAGCAT CTTTCTACTA 
GCTTTGGCAA CCGTGCCCCT TCTGGGTGCC ACCTGCTGTC ATTTGCTCTA CAAGTATCAA 
GGCAAGACCT ACAAGCTGAT TATGAAAACA CAAAACCCAG ATCAGATGGA AAAGAACATA 
GCGACAGATT TTGATCCCAG GGCGTCATTT GTGGGATCCG AAAAGAATTT TAACCCCAGT 
GAGATGGGAG AGGGAGAAGG CGAGGCTGAT GGCGAGGAGG GAGACGGAGA AGGAGAGGAC 
ATCGAGGGCA GTGTGGTGAC CGAGTCCATT CCAGAGTCAC AGTCCAAAAC ACAAGAGGAG 
TTTGAAGTCG GTTCCGAGTA CAGCGATCGG TTACCCCTTG GAGCTGAAGC AGTAAACATA 
TCAGCGGAGA TCAACGGTAA TTACAAACAA CCACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISLR2 ( XM_009298031.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296306.1 Danio rerio immunoglobulin superfamily containing leucine-rich repeat 2 (islr2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41618
Clone ID Related Accession (Same CDS sequence)	XM_009298031.2
Accession Version	XM_009298031.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	immunoglobulin superfamily containing leucine-rich repeat protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACCA AATACCTGAT GCTTATTGCC CTGGGGACTG CATTGATTGG CAATGCACAC 
TGTTGCCCCA AGCAGTGTGC CTGCTCTGAT AAATACAACC ACCAGTTCGT AGACTGTGCC 
TACAAAGACC TTGTGGAAGT ACCTGTGGGT TTGCCGTCCA ATGCAAGCAC CCTGAGTCTT 
TCAGCGAACA AGATTAAAGT GCTGAAATCC AAAACTTTTG TAAACGTGAC CCAGGTGACC 
TCTCTTTGGC TGGCTCACAA TGAAATCATC ACAGTCGAGA GGGACACGTT GGCTCCATTG 
ATTCAGCTGA AAAATTTGGA TATTAGCAAC AACAAAATTG TGCATTTTCC ATGGGAGGAC 
TTGGCCAATC TTGGTGCTTT GCAGTTGTTA AAGATGAACA ATAATGAGAT GGTCAGCATC 
CCTAAGAACG CCTTTTCAAA CTTGAAAGAC CTGCGTTCAA TCCGTATTAA CAATAACAAG 
TTTACCACTA TTGTGCAAGG AACATTTGAT TCTTTGGCTG CCATGTCCCA TCTGCAAATC 
TTCCACAACC CCTTCATCTG CTCCTGCAAC CTTGAGTGGC TTAGGGACTG GATCCTCAAA 
TCCTCAATCT CCATTCCTGA ACAAAACAAC ATCGCTTGCG ATGCCCCATC CCACCTCAAA 
GGTTCTCAGG TCACTAGCAT GCCCAAATTG AATTGCAAGG CCCCATCTGT ATCCATTACA 
TATCAGCCAA ACATAGAAAA GTCTGAACTC TATGAGGGAT ATATGGTTAT GCTATACTGC 
GAAACAAAAG GAACACCCAA ACCCGAGGTC ACATGGGAGA TATTTGCTGG AAACCAACTG 
ATTACATTCC CTTTGCCTGC TATTGTTGAA AAAACTGACA TTCCGATTAA TGGCCCACCT 
ACAAACACCA GGTTCCTCGT GTTTCAAAAT GGCACCCTGA TCGTTCCTCG CATGAGCAAG 
AAAGAAGAGG GAAACTACAC CTGTTCTGCT GTTAATGACA TGGGAAAAGC TGAGAGCTCA 
GTTAGACTTG TTGTCGCAGG CACTAAAAAG CATGCCACCA ATTCTATGCT GGACACGAAA 
TCACCAGGGC TTTTACCAGA AGACAAACTC GGTTCAAAAG GCTCCAAAAA CAGCGTGATT 
AGCATGAGGC CTAAATTTGA AGAGAAGACT AAAAGTCTTC CCACTGGCAC ATCTGTCATT 
ACAGTGGATA AAGAGCAAGT AGATAAAGGG GCTGATACTT TGCCATTTGT TGGTAAATGT 
GGAATAAATG ACGGCACACA GTACATTTCT AACCATGCTT TCAACCTCAG CTTGGATGAA 
CTTAAACAGT ACACATTTGA TTTTGGGGTT ATCGCTCTGG AAGTTTCAGA GACTGAGGCT 
AAAGTTCAGC TGAACCCTAT GCAGATGGCC AACGCAAAGT CAAACATTCA CCTCAGTCAA 
CAACAAGACC TCCAAACTGT GAATAAGGAG CCTTTTAGTC TTTTCCAAAC ATCCGCCAAA 
AAGTCACCAC TAGACTTGTT GTACCTTTGT GTCAGTACTG GCAATGGACA TTCAGTGGTG 
CAGTGGTCCA AGATAGAAGA AGGCGTCAAC GCCTATCGCT TTCAGCACCT CAAGCCAGGC 
ACCAATTACA CCCTCTGTCT CACCTACGGA GGCCAAGACT GCCAAGTCCA AGTGGTCTTC 
ACAACCCGAA AGAAAATCCC ATCCTTGTTA ATCATTGTAG TCGTTAGCAT CTTTCTACTA 
GCTTTGGCAA CCGTGCCCCT TCTGGGTGCC ACCTGCTGTC ATTTGCTCTA CAAGTATCAA 
GGCAAGACCT ACAAGCTGAT TATGAAAACA CAAAACCCAG ATCAGATGGA AAAGAACATA 
GCGACAGATT TTGATCCCAG GGCGTCATTT GTGGGATCCG AAAAGAATTT TAACCCCAGT 
GAGATGGGAG AGGGAGAAGG CGAGGCTGAT GGCGAGGAGG GAGACGGAGA AGGAGAGGAC 
ATCGAGGGCA GTGTGGTGAC CGAGTCCATT CCAGAGTCAC AGTCCAAAAC ACAAGAGGAG 
TTTGAAGTCG GTTCCGAGTA CAGCGATCGG TTACCCCTTG GAGCTGAAGC AGTAAACATA 
TCAGCGGAGA TCAACGGTAA TTACAAACAA CCACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296306.1
CDS	286..2424
Translation	MATKYLMLIALGTALIGNAHCCPKQCACSDKYNHQFVDCAYKDLVEVPVGLPSNASTLSLSANKIKVLKSKTFVNVTQVTSLWLAHNEIITVERDTLAPLIQLKNLDISNNKIVHFPWEDLANLGALQLLKMNNNEMVSIPKNAFSNLKDLRSIRINNNKFTTIVQGTFDSLAAMSHLQIFHNPFICSCNLEWLRDWILKSSISIPEQNNIACDAPSHLKGSQVTSMPKLNCKAPSVSITYQPNIEKSELYEGYMVMLYCETKGTPKPEVTWEIFAGNQLITFPLPAIVEKTDIPINGPPTNTRFLVFQNGTLIVPRMSKKEEGNYTCSAVNDMGKAESSVRLVVAGTKKHATNSMLDTKSPGLLPEDKLGSKGSKNSVISMRPKFEEKTKSLPTGTSVITVDKEQVDKGADTLPFVGKCGINDGTQYISNHAFNLSLDELKQYTFDFGVIALEVSETEAKVQLNPMQMANAKSNIHLSQQQDLQTVNKEPFSLFQTSAKKSPLDLLYLCVSTGNGHSVVQWSKIEEGVNAYRFQHLKPGTNYTLCLTYGGQDCQVQVVFTTRKKIPSLLIIVVVSIFLLALATVPLLGATCCHLLYKYQGKTYKLIMKTQNPDQMEKNIATDFDPRASFVGSEKNFNPSEMGEGEGEADGEEGDGEGEDIEGSVVTESIPESQSKTQEEFEVGSEYSDRLPLGAEAVNISAEINGNYKQPR

Target ORF information:

RefSeq Version	XM_009298031.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio immunoglobulin superfamily containing leucine-rich repeat 2 (islr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298031.2

ORF Insert Sequence:

ATGGCAACCA AATACCTGAT GCTTATTGCC CTGGGGACTG CATTGATTGG CAATGCACAC 
TGTTGCCCCA AGCAGTGTGC CTGCTCTGAT AAATACAACC ACCAGTTCGT AGACTGTGCC 
TACAAAGACC TTGTGGAAGT ACCTGTGGGT TTGCCGTCCA ATGCAAGCAC CCTGAGTCTT 
TCAGCGAACA AGATTAAAGT GCTGAAATCC AAAACTTTTG TAAACGTGAC CCAGGTGACC 
TCTCTTTGGC TGGCTCACAA TGAAATCATC ACAGTCGAGA GGGACACGTT GGCTCCATTG 
ATTCAGCTGA AAAATTTGGA TATTAGCAAC AACAAAATTG TGCATTTTCC ATGGGAGGAC 
TTGGCCAATC TTGGTGCTTT GCAGTTGTTA AAGATGAACA ATAATGAGAT GGTCAGCATC 
CCTAAGAACG CCTTTTCAAA CTTGAAAGAC CTGCGTTCAA TCCGTATTAA CAATAACAAG 
TTTACCACTA TTGTGCAAGG AACATTTGAT TCTTTGGCTG CCATGTCCCA TCTGCAAATC 
TTCCACAACC CCTTCATCTG CTCCTGCAAC CTTGAGTGGC TTAGGGACTG GATCCTCAAA 
TCCTCAATCT CCATTCCTGA ACAAAACAAC ATCGCTTGCG ATGCCCCATC CCACCTCAAA 
GGTTCTCAGG TCACTAGCAT GCCCAAATTG AATTGCAAGG CCCCATCTGT ATCCATTACA 
TATCAGCCAA ACATAGAAAA GTCTGAACTC TATGAGGGAT ATATGGTTAT GCTATACTGC 
GAAACAAAAG GAACACCCAA ACCCGAGGTC ACATGGGAGA TATTTGCTGG AAACCAACTG 
ATTACATTCC CTTTGCCTGC TATTGTTGAA AAAACTGACA TTCCGATTAA TGGCCCACCT 
ACAAACACCA GGTTCCTCGT GTTTCAAAAT GGCACCCTGA TCGTTCCTCG CATGAGCAAG 
AAAGAAGAGG GAAACTACAC CTGTTCTGCT GTTAATGACA TGGGAAAAGC TGAGAGCTCA 
GTTAGACTTG TTGTCGCAGG CACTAAAAAG CATGCCACCA ATTCTATGCT GGACACGAAA 
TCACCAGGGC TTTTACCAGA AGACAAACTC GGTTCAAAAG GCTCCAAAAA CAGCGTGATT 
AGCATGAGGC CTAAATTTGA AGAGAAGACT AAAAGTCTTC CCACTGGCAC ATCTGTCATT 
ACAGTGGATA AAGAGCAAGT AGATAAAGGG GCTGATACTT TGCCATTTGT TGGTAAATGT 
GGAATAAATG ACGGCACACA GTACATTTCT AACCATGCTT TCAACCTCAG CTTGGATGAA 
CTTAAACAGT ACACATTTGA TTTTGGGGTT ATCGCTCTGG AAGTTTCAGA GACTGAGGCT 
AAAGTTCAGC TGAACCCTAT GCAGATGGCC AACGCAAAGT CAAACATTCA CCTCAGTCAA 
CAACAAGACC TCCAAACTGT GAATAAGGAG CCTTTTAGTC TTTTCCAAAC ATCCGCCAAA 
AAGTCACCAC TAGACTTGTT GTACCTTTGT GTCAGTACTG GCAATGGACA TTCAGTGGTG 
CAGTGGTCCA AGATAGAAGA AGGCGTCAAC GCCTATCGCT TTCAGCACCT CAAGCCAGGC 
ACCAATTACA CCCTCTGTCT CACCTACGGA GGCCAAGACT GCCAAGTCCA AGTGGTCTTC 
ACAACCCGAA AGAAAATCCC ATCCTTGTTA ATCATTGTAG TCGTTAGCAT CTTTCTACTA 
GCTTTGGCAA CCGTGCCCCT TCTGGGTGCC ACCTGCTGTC ATTTGCTCTA CAAGTATCAA 
GGCAAGACCT ACAAGCTGAT TATGAAAACA CAAAACCCAG ATCAGATGGA AAAGAACATA 
GCGACAGATT TTGATCCCAG GGCGTCATTT GTGGGATCCG AAAAGAATTT TAACCCCAGT 
GAGATGGGAG AGGGAGAAGG CGAGGCTGAT GGCGAGGAGG GAGACGGAGA AGGAGAGGAC 
ATCGAGGGCA GTGTGGTGAC CGAGTCCATT CCAGAGTCAC AGTCCAAAAC ACAAGAGGAG 
TTTGAAGTCG GTTCCGAGTA CAGCGATCGG TTACCCCTTG GAGCTGAAGC AGTAAACATA 
TCAGCGGAGA TCAACGGTAA TTACAAACAA CCACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The LRR receptor Islr2 is required for retinal axon routing at the vertebrate optic chiasm.
Neural development1023(2015 Oct)
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ISLR2 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

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ORF Insert Sequence:

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