usp25 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009305557.2	XP_009303832.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_009305558.2	XP_009303833.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
XM_005169156.3	XP_005169213.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
XM_005169157.3	XP_005169214.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
XM_005169161.3	XP_005169218.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
XM_005169157.4	XP_005169214.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
XM_009305560.3	XP_009303835.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
XM_021479151.1	XP_021334826.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
XM_009305560.2	XP_009303835.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
XM_009305559.2	XP_009303834.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
XM_009305557.3	XP_009303832.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_009305558.3	XP_009303833.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
NM_001001886.1	NP_001001886.1	ubiquitin carboxyl-terminal hydrolase 25
XM_009305559.3	XP_009303834.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
XM_005169156.4	XP_005169213.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
XM_005169161.4	XP_005169218.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa147600	XM_021479151.1 Latest version!	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa03317	NM_001001886.1 Latest version!	Danio rerio ubiquitin specific peptidase 25 (usp25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa16368	XM_005169156.4 Latest version!	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa16369	XM_005169157.4 Latest version!	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa16373	XM_005169161.4 Latest version!	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40921	XM_009305557.3 Latest version!	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa40922	XM_009305558.3 Latest version!	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40923	XM_009305559.3 Latest version!	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40924	XM_009305560.3 Latest version!	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa16369	XM_005169157.3	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40924	XM_009305560.2	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40921	XM_009305557.2	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa40922	XM_009305558.2	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40923	XM_009305559.2	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa16368	XM_005169156.3	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa16373	XM_005169161.3	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

usp25 ( XM_021479151.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334826.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa147600
Clone ID Related Accession (Same CDS sequence)	XM_021479151.1
Accession Version	XM_021479151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCATCA GCAGCTCCTC AGCATGACCC AGAGTACATG 
GAGCAACAGT CGCCCAGCGG AGACTCCAAA CACCTGCAGG AGGACACAGA GAGAGCCATC 
AGCAAGGCTG CAGCGGAGCA TGATGAGAAG AGTCCAGAAG CTCTGCTTAA TACAGCGATG 
ATGACCCCAA ACATGCAGGG TATCATAATG GCCATTGGCA AATCCAGGAG TGTTTATGAC 
AAGTGTGGGC CTGAAGCAGC GTTCTTCAAG GCGATGAAGG TTGAGTACAC GCGTTTGCTG 
AGGCTTGCTC AGGAGGACAC GCCGCCCGAG AGAGACTACC GGCTACAGCA TGTCATTGTG 
TTCTTTATTC ACAATGAGGC GCCCAAGAAG ATTGTGGAGA GAACATTGCT CATGCAGTTT 
GCTGACAGAA ACCTTGGCTT CGATGAGAGG TGTAAAAGCA TCATGAAAGT GGCACGTGCC 
AAACTGGAGC TCATCAAGCC TGATGAGGTG AACTTGGAGG AGTACGAGAT GTGGCATCAG 
GACTACAGGA TTTTCCGTGA GACGTCAGTG TTCCTGTTGA TCGGCCTGGA ATTATTCATG 
AAGAAAAGTT TTGTGGAAGC GTTGATGTAT CTGATCTACG CCTATCAGTA CAACAAAGAG 
CTTCTGGCGA AAGGGCCGTA CAGAGGCCAT GATGAGGAGC TGATCGCACA TTACCGCAGA 
GAGTGCCTGC TGAAGTTAAA TGAATACGCT GCCGCTTTGT TCGAGACGGG CGAGGAGGCT 
AAAGTGAACA CGGGTCTGAG CATCATGAAC GATCTGGTGG TGCCCTGTCT TCCTCTGTTA 
CTGGTGGATG AGATGGAGGA GAAGGACATG GTGGCTGTTG AAGACATGAG GAACCGATGG 
TGCTCATACC TGGGCCAGGA AATGGAGCCG AATCTACAGG AGAAGCTGAC CGACTTCCTC 
CCCAAACTGC TGGACTGCTC GACCGAGATC AAAAGCTTTC ACGAGCCGCC CAAACTGCCC 
TCTTACTCCA CGCTGGAGCT GTTCGAGCGC TTCGGCAGGG TCATGACGTC CCTCACTATG 
GGTCGAACCC CTGCAGATGG CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334826.1
CDS	209..3535
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPSAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_021479151.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479151.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCATCA GCAGCTCCTC AGCATGACCC AGAGTACATG 
GAGCAACAGT CGCCCAGCGG AGACTCCAAA CACCTGCAGG AGGACACAGA GAGAGCCATC 
AGCAAGGCTG CAGCGGAGCA TGATGAGAAG AGTCCAGAAG CTCTGCTTAA TACAGCGATG 
ATGACCCCAA ACATGCAGGG TATCATAATG GCCATTGGCA AATCCAGGAG TGTTTATGAC 
AAGTGTGGGC CTGAAGCAGC GTTCTTCAAG GCGATGAAGG TTGAGTACAC GCGTTTGCTG 
AGGCTTGCTC AGGAGGACAC GCCGCCCGAG AGAGACTACC GGCTACAGCA TGTCATTGTG 
TTCTTTATTC ACAATGAGGC GCCCAAGAAG ATTGTGGAGA GAACATTGCT CATGCAGTTT 
GCTGACAGAA ACCTTGGCTT CGATGAGAGG TGTAAAAGCA TCATGAAAGT GGCACGTGCC 
AAACTGGAGC TCATCAAGCC TGATGAGGTG AACTTGGAGG AGTACGAGAT GTGGCATCAG 
GACTACAGGA TTTTCCGTGA GACGTCAGTG TTCCTGTTGA TCGGCCTGGA ATTATTCATG 
AAGAAAAGTT TTGTGGAAGC GTTGATGTAT CTGATCTACG CCTATCAGTA CAACAAAGAG 
CTTCTGGCGA AAGGGCCGTA CAGAGGCCAT GATGAGGAGC TGATCGCACA TTACCGCAGA 
GAGTGCCTGC TGAAGTTAAA TGAATACGCT GCCGCTTTGT TCGAGACGGG CGAGGAGGCT 
AAAGTGAACA CGGGTCTGAG CATCATGAAC GATCTGGTGG TGCCCTGTCT TCCTCTGTTA 
CTGGTGGATG AGATGGAGGA GAAGGACATG GTGGCTGTTG AAGACATGAG GAACCGATGG 
TGCTCATACC TGGGCCAGGA AATGGAGCCG AATCTACAGG AGAAGCTGAC CGACTTCCTC 
CCCAAACTGC TGGACTGCTC GACCGAGATC AAAAGCTTTC ACGAGCCGCC CAAACTGCCC 
TCTTACTCCA CGCTGGAGCT GTTCGAGCGC TTCGGCAGGG TCATGACGTC CCTCACTATG 
GGTCGAACCC CTGCAGATGG CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( NM_001001886.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001001886.1 Danio rerio ubiquitin specific peptidase 25 (usp25), mRNA.

Price & Availability↑

CloneID	ODa03317
Clone ID Related Accession (Same CDS sequence)	NM_001001886.1
Accession Version	NM_001001886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045454.1. On Jul 6, 2005 this sequence version replaced XM_696293.1. ##Evidence-Data-START## Transcript exon combination :: BC045454.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAAGTG 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACTAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGGATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACTCA TAAACTTCTG GACTGGCTGG AGGACGCGTT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACCGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCAATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGTT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTGAC CGTCCTCCAG CAGCGACTCG AGAGGTATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACGATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAACAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACTAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACCATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCTCAG CATGACCCAG AGTACATGGA GCAACAGTCG 
CCCAGCGGAG ACTCCAAACA CCTGCAGGAG GACACAGAGA GAGCCATCAG CAAGGCTGCA 
GCGGAGCATG ATGAGAAGAG TCCAGAAGCC CTGCTTAATA CAGCGATGAA GGTTGAGTAC 
ACGCGTTTGC TGAGGCTTGC TCAGGAGGAC ACGCCGCCCG AGAGAGACTA CCGGCTACAG 
CATGTCATTG TGTTCTTTAT TCACAATGAG GCGCCCAAGA AGATTGTGGA GAGAACATTG 
CTCATGCAGT TTGCTGACAG AAACCTTGGC TTCGATGAGA GGTGTAAAAG CATCATGAAA 
GTGGCACGTG CCAAACTGGA GCTCATCAAG CCTGATGAGG TGAACATGGA GGAGTACGAG 
ATGTGGCATC AGGACTACAG GATTTTCCGT GAGACGTCAG TGTTCCTGTT GATCGGCCTG 
GAATTATTCA TGAAGAAAAG TTTTGTGGAA GCGTTGATGT ATCTGATCTA CGCCTATCAG 
TACAACAAAG AGCTTCTGGC GAAAGGGCCG TACAGAGGCC ATGATGAGGA GCTGATCGCA 
CATTACCGCA GAGAGTGCCT GCTGAAGTTA AATGAATACG CTGCCGCTTT GTTCGAGACG 
GGCGAGGAGG CTAAAGTGAA CACGGGTCTG AGCATCATGA ACGATCTGGT GGTGCCCTGT 
CTTCCTCTGT TACTGGTGGA TGAGATGGAG GAGAAGGACA TGGTGGCTGT TGAAGACATG 
AGGAACCGAT GGTGCTCATA CCTGGGCCAG GAAATGGAGC CGAATCTACA GGAGAAGCTG 
ACCGACTTCC TCCCCAAACT GCTGGACTGC TCGACCGAGA TCAAAAGCTT TCACGAGCCG 
CCCAAACTGC CCTCTTACTC CACGCTGGAG CTGTTCGAGC GCTTCGGCAG GGTCATGACG 
TCCCTCACTA TGGGTCGAAC CCCTGCAGAT GGCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001886.1
CDS	171..3389
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDSYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNMEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	NM_001001886.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001886.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAAGTG 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACTAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGGATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACTCA TAAACTTCTG GACTGGCTGG AGGACGCGTT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACCGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCAATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGTT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTGAC CGTCCTCCAG CAGCGACTCG AGAGGTATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACGATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAACAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACTAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACCATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCTCAG CATGACCCAG AGTACATGGA GCAACAGTCG 
CCCAGCGGAG ACTCCAAACA CCTGCAGGAG GACACAGAGA GAGCCATCAG CAAGGCTGCA 
GCGGAGCATG ATGAGAAGAG TCCAGAAGCC CTGCTTAATA CAGCGATGAA GGTTGAGTAC 
ACGCGTTTGC TGAGGCTTGC TCAGGAGGAC ACGCCGCCCG AGAGAGACTA CCGGCTACAG 
CATGTCATTG TGTTCTTTAT TCACAATGAG GCGCCCAAGA AGATTGTGGA GAGAACATTG 
CTCATGCAGT TTGCTGACAG AAACCTTGGC TTCGATGAGA GGTGTAAAAG CATCATGAAA 
GTGGCACGTG CCAAACTGGA GCTCATCAAG CCTGATGAGG TGAACATGGA GGAGTACGAG 
ATGTGGCATC AGGACTACAG GATTTTCCGT GAGACGTCAG TGTTCCTGTT GATCGGCCTG 
GAATTATTCA TGAAGAAAAG TTTTGTGGAA GCGTTGATGT ATCTGATCTA CGCCTATCAG 
TACAACAAAG AGCTTCTGGC GAAAGGGCCG TACAGAGGCC ATGATGAGGA GCTGATCGCA 
CATTACCGCA GAGAGTGCCT GCTGAAGTTA AATGAATACG CTGCCGCTTT GTTCGAGACG 
GGCGAGGAGG CTAAAGTGAA CACGGGTCTG AGCATCATGA ACGATCTGGT GGTGCCCTGT 
CTTCCTCTGT TACTGGTGGA TGAGATGGAG GAGAAGGACA TGGTGGCTGT TGAAGACATG 
AGGAACCGAT GGTGCTCATA CCTGGGCCAG GAAATGGAGC CGAATCTACA GGAGAAGCTG 
ACCGACTTCC TCCCCAAACT GCTGGACTGC TCGACCGAGA TCAAAAGCTT TCACGAGCCG 
CCCAAACTGC CCTCTTACTC CACGCTGGAG CTGTTCGAGC GCTTCGGCAG GGTCATGACG 
TCCCTCACTA TGGGTCGAAC CCCTGCAGAT GGCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_005169156.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169213.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa16368
Clone ID Related Accession (Same CDS sequence)	XM_005169156.3 , XM_005169156.4
Accession Version	XM_005169156.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169156.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCAGCTCCT CAGCATGACC CAGAGTACAT GGAGCAACAG 
TCGCCCAGCG GAGACTCCAA ACACCTGCAG GAGGACACAG AGAGAGCCAT CAGCAAGGCT 
GCAGCGGAGC ATGATGAGAA GAGTCCAGAA GCTCTGCTTA ATACATCTCA GTCATCTCAC 
CCTGACAGCA AGCTGACCTC TGACCCCTGC CCTCCTCCTG ACCCCGAGCA GGACGACCCC 
ACCTGCCCTC CACCCGGCCC TCAGCGGGTG GTGGAGGTGG CCATTCCTAA TGTAGGGACC 
TTCGTCATTC AGTCGAAGGA GGGAGGTTAT GATGACGAGG CGATGATGAC CCCAAACATG 
CAGGGTATCA TAATGGCCAT TGGCAAATCC AGGAGTGTTT ATGACAAGTG TGGGCCTGAA 
GCAGCGTTCT TCAAGGCGAT GAAGGTTGAG TACACGCGTT TGCTGAGGCT TGCTCAGGAG 
GACACGCCGC CCGAGAGAGA CTACCGGCTA CAGCATGTCA TTGTGTTCTT TATTCACAAT 
GAGGCGCCCA AGAAGATTGT GGAGAGAACA TTGCTCATGC AGTTTGCTGA CAGAAACCTT 
GGCTTCGATG AGAGGTGTAA AAGCATCATG AAAGTGGCAC GTGCCAAACT GGAGCTCATC 
AAGCCTGATG AGGTGAACTT GGAGGAGTAC GAGATGTGGC ATCAGGACTA CAGGATTTTC 
CGTGAGACGT CAGTGTTCCT GTTGATCGGC CTGGAATTAT TCATGAAGAA AAGTTTTGTG 
GAAGCGTTGA TGTATCTGAT CTACGCCTAT CAGTACAACA AAGAGCTTCT GGCGAAAGGG 
CCGTACAGAG GCCATGATGA GGAGCTGATC GCACATTACC GCAGAGAGTG CCTGCTGAAG 
TTAAATGAAT ACGCTGCCGC TTTGTTCGAG ACGGGCGAGG AGGCTAAAGT GAACACGGGT 
CTGAGCATCA TGAACGATCT GGTGGTGCCC TGTCTTCCTC TGTTACTGGT GGATGAGATG 
GAGGAGAAGG ACATGGTGGC TGTTGAAGAC ATGAGGAACC GATGGTGCTC ATACCTGGGC 
CAGGAAATGG AGCCGAATCT ACAGGAGAAG CTGACCGACT TCCTCCCCAA ACTGCTGGAC 
TGCTCGACCG AGATCAAAAG CTTTCACGAG CCGCCCAAAC TGCCCTCTTA CTCCACGCTG 
GAGCTGTTCG AGCGCTTCGG CAGGGTCATG ACGTCCCTCA CTATGGGTCG AACCCCTGCA 
GATGGCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169213.1
CDS	207..3698
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTSQSSHPDSKLTSDPCPPPDPEQDDPTCPPPGPQRVVEVAIPNVGTFVIQSKEGGYDDEAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_005169156.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169156.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCAGCTCCT CAGCATGACC CAGAGTACAT GGAGCAACAG 
TCGCCCAGCG GAGACTCCAA ACACCTGCAG GAGGACACAG AGAGAGCCAT CAGCAAGGCT 
GCAGCGGAGC ATGATGAGAA GAGTCCAGAA GCTCTGCTTA ATACATCTCA GTCATCTCAC 
CCTGACAGCA AGCTGACCTC TGACCCCTGC CCTCCTCCTG ACCCCGAGCA GGACGACCCC 
ACCTGCCCTC CACCCGGCCC TCAGCGGGTG GTGGAGGTGG CCATTCCTAA TGTAGGGACC 
TTCGTCATTC AGTCGAAGGA GGGAGGTTAT GATGACGAGG CGATGATGAC CCCAAACATG 
CAGGGTATCA TAATGGCCAT TGGCAAATCC AGGAGTGTTT ATGACAAGTG TGGGCCTGAA 
GCAGCGTTCT TCAAGGCGAT GAAGGTTGAG TACACGCGTT TGCTGAGGCT TGCTCAGGAG 
GACACGCCGC CCGAGAGAGA CTACCGGCTA CAGCATGTCA TTGTGTTCTT TATTCACAAT 
GAGGCGCCCA AGAAGATTGT GGAGAGAACA TTGCTCATGC AGTTTGCTGA CAGAAACCTT 
GGCTTCGATG AGAGGTGTAA AAGCATCATG AAAGTGGCAC GTGCCAAACT GGAGCTCATC 
AAGCCTGATG AGGTGAACTT GGAGGAGTAC GAGATGTGGC ATCAGGACTA CAGGATTTTC 
CGTGAGACGT CAGTGTTCCT GTTGATCGGC CTGGAATTAT TCATGAAGAA AAGTTTTGTG 
GAAGCGTTGA TGTATCTGAT CTACGCCTAT CAGTACAACA AAGAGCTTCT GGCGAAAGGG 
CCGTACAGAG GCCATGATGA GGAGCTGATC GCACATTACC GCAGAGAGTG CCTGCTGAAG 
TTAAATGAAT ACGCTGCCGC TTTGTTCGAG ACGGGCGAGG AGGCTAAAGT GAACACGGGT 
CTGAGCATCA TGAACGATCT GGTGGTGCCC TGTCTTCCTC TGTTACTGGT GGATGAGATG 
GAGGAGAAGG ACATGGTGGC TGTTGAAGAC ATGAGGAACC GATGGTGCTC ATACCTGGGC 
CAGGAAATGG AGCCGAATCT ACAGGAGAAG CTGACCGACT TCCTCCCCAA ACTGCTGGAC 
TGCTCGACCG AGATCAAAAG CTTTCACGAG CCGCCCAAAC TGCCCTCTTA CTCCACGCTG 
GAGCTGTTCG AGCGCTTCGG CAGGGTCATG ACGTCCCTCA CTATGGGTCG AACCCCTGCA 
GATGGCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_005169156.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169213.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa16368
Clone ID Related Accession (Same CDS sequence)	XM_005169156.3 , XM_005169156.4
Accession Version	XM_005169156.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169156.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCAGCTCCT CAGCATGACC CAGAGTACAT GGAGCAACAG 
TCGCCCAGCG GAGACTCCAA ACACCTGCAG GAGGACACAG AGAGAGCCAT CAGCAAGGCT 
GCAGCGGAGC ATGATGAGAA GAGTCCAGAA GCTCTGCTTA ATACATCTCA GTCATCTCAC 
CCTGACAGCA AGCTGACCTC TGACCCCTGC CCTCCTCCTG ACCCCGAGCA GGACGACCCC 
ACCTGCCCTC CACCCGGCCC TCAGCGGGTG GTGGAGGTGG CCATTCCTAA TGTAGGGACC 
TTCGTCATTC AGTCGAAGGA GGGAGGTTAT GATGACGAGG CGATGATGAC CCCAAACATG 
CAGGGTATCA TAATGGCCAT TGGCAAATCC AGGAGTGTTT ATGACAAGTG TGGGCCTGAA 
GCAGCGTTCT TCAAGGCGAT GAAGGTTGAG TACACGCGTT TGCTGAGGCT TGCTCAGGAG 
GACACGCCGC CCGAGAGAGA CTACCGGCTA CAGCATGTCA TTGTGTTCTT TATTCACAAT 
GAGGCGCCCA AGAAGATTGT GGAGAGAACA TTGCTCATGC AGTTTGCTGA CAGAAACCTT 
GGCTTCGATG AGAGGTGTAA AAGCATCATG AAAGTGGCAC GTGCCAAACT GGAGCTCATC 
AAGCCTGATG AGGTGAACTT GGAGGAGTAC GAGATGTGGC ATCAGGACTA CAGGATTTTC 
CGTGAGACGT CAGTGTTCCT GTTGATCGGC CTGGAATTAT TCATGAAGAA AAGTTTTGTG 
GAAGCGTTGA TGTATCTGAT CTACGCCTAT CAGTACAACA AAGAGCTTCT GGCGAAAGGG 
CCGTACAGAG GCCATGATGA GGAGCTGATC GCACATTACC GCAGAGAGTG CCTGCTGAAG 
TTAAATGAAT ACGCTGCCGC TTTGTTCGAG ACGGGCGAGG AGGCTAAAGT GAACACGGGT 
CTGAGCATCA TGAACGATCT GGTGGTGCCC TGTCTTCCTC TGTTACTGGT GGATGAGATG 
GAGGAGAAGG ACATGGTGGC TGTTGAAGAC ATGAGGAACC GATGGTGCTC ATACCTGGGC 
CAGGAAATGG AGCCGAATCT ACAGGAGAAG CTGACCGACT TCCTCCCCAA ACTGCTGGAC 
TGCTCGACCG AGATCAAAAG CTTTCACGAG CCGCCCAAAC TGCCCTCTTA CTCCACGCTG 
GAGCTGTTCG AGCGCTTCGG CAGGGTCATG ACGTCCCTCA CTATGGGTCG AACCCCTGCA 
GATGGCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169213.1
CDS	210..3701
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTSQSSHPDSKLTSDPCPPPDPEQDDPTCPPPGPQRVVEVAIPNVGTFVIQSKEGGYDDEAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_005169156.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169156.4

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCAGCTCCT CAGCATGACC CAGAGTACAT GGAGCAACAG 
TCGCCCAGCG GAGACTCCAA ACACCTGCAG GAGGACACAG AGAGAGCCAT CAGCAAGGCT 
GCAGCGGAGC ATGATGAGAA GAGTCCAGAA GCTCTGCTTA ATACATCTCA GTCATCTCAC 
CCTGACAGCA AGCTGACCTC TGACCCCTGC CCTCCTCCTG ACCCCGAGCA GGACGACCCC 
ACCTGCCCTC CACCCGGCCC TCAGCGGGTG GTGGAGGTGG CCATTCCTAA TGTAGGGACC 
TTCGTCATTC AGTCGAAGGA GGGAGGTTAT GATGACGAGG CGATGATGAC CCCAAACATG 
CAGGGTATCA TAATGGCCAT TGGCAAATCC AGGAGTGTTT ATGACAAGTG TGGGCCTGAA 
GCAGCGTTCT TCAAGGCGAT GAAGGTTGAG TACACGCGTT TGCTGAGGCT TGCTCAGGAG 
GACACGCCGC CCGAGAGAGA CTACCGGCTA CAGCATGTCA TTGTGTTCTT TATTCACAAT 
GAGGCGCCCA AGAAGATTGT GGAGAGAACA TTGCTCATGC AGTTTGCTGA CAGAAACCTT 
GGCTTCGATG AGAGGTGTAA AAGCATCATG AAAGTGGCAC GTGCCAAACT GGAGCTCATC 
AAGCCTGATG AGGTGAACTT GGAGGAGTAC GAGATGTGGC ATCAGGACTA CAGGATTTTC 
CGTGAGACGT CAGTGTTCCT GTTGATCGGC CTGGAATTAT TCATGAAGAA AAGTTTTGTG 
GAAGCGTTGA TGTATCTGAT CTACGCCTAT CAGTACAACA AAGAGCTTCT GGCGAAAGGG 
CCGTACAGAG GCCATGATGA GGAGCTGATC GCACATTACC GCAGAGAGTG CCTGCTGAAG 
TTAAATGAAT ACGCTGCCGC TTTGTTCGAG ACGGGCGAGG AGGCTAAAGT GAACACGGGT 
CTGAGCATCA TGAACGATCT GGTGGTGCCC TGTCTTCCTC TGTTACTGGT GGATGAGATG 
GAGGAGAAGG ACATGGTGGC TGTTGAAGAC ATGAGGAACC GATGGTGCTC ATACCTGGGC 
CAGGAAATGG AGCCGAATCT ACAGGAGAAG CTGACCGACT TCCTCCCCAA ACTGCTGGAC 
TGCTCGACCG AGATCAAAAG CTTTCACGAG CCGCCCAAAC TGCCCTCTTA CTCCACGCTG 
GAGCTGTTCG AGCGCTTCGG CAGGGTCATG ACGTCCCTCA CTATGGGTCG AACCCCTGCA 
GATGGCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_005169157.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169214.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa16369
Clone ID Related Accession (Same CDS sequence)	XM_005169157.3 , XM_005169157.4
Accession Version	XM_005169157.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169157.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCTCAG CATGACCCAG AGTACATGGA GCAACAGTCG 
CCCAGCGGAG ACTCCAAACA CCTGCAGGAG GACACAGAGA GAGCCATCAG CAAGGCTGCA 
GCGGAGCATG ATGAGAAGAG TCCAGAAGCT CTGCTTAATA CATCTCAGTC ATCTCACCCT 
GACAGCAAGC TGACCTCTGA CCCCTGCCCT CCTCCTGACC CCGAGCAGGA CGACCCCACC 
TGCCCTCCAC CCGGCCCTCA GCGGGTGGTG GAGGTGGCCA TTCCTAATGT AGGGACCTTC 
GTCATTCAGT CGAAGGAGGG AGGTTATGAT GACGAGGCGA TGATGACCCC AAACATGCAG 
GGTATCATAA TGGCCATTGG CAAATCCAGG AGTGTTTATG ACAAGTGTGG GCCTGAAGCA 
GCGTTCTTCA AGGCGATGAA GGTTGAGTAC ACGCGTTTGC TGAGGCTTGC TCAGGAGGAC 
ACGCCGCCCG AGAGAGACTA CCGGCTACAG CATGTCATTG TGTTCTTTAT TCACAATGAG 
GCGCCCAAGA AGATTGTGGA GAGAACATTG CTCATGCAGT TTGCTGACAG AAACCTTGGC 
TTCGATGAGA GGTGTAAAAG CATCATGAAA GTGGCACGTG CCAAACTGGA GCTCATCAAG 
CCTGATGAGG TGAACTTGGA GGAGTACGAG ATGTGGCATC AGGACTACAG GATTTTCCGT 
GAGACGTCAG TGTTCCTGTT GATCGGCCTG GAATTATTCA TGAAGAAAAG TTTTGTGGAA 
GCGTTGATGT ATCTGATCTA CGCCTATCAG TACAACAAAG AGCTTCTGGC GAAAGGGCCG 
TACAGAGGCC ATGATGAGGA GCTGATCGCA CATTACCGCA GAGAGTGCCT GCTGAAGTTA 
AATGAATACG CTGCCGCTTT GTTCGAGACG GGCGAGGAGG CTAAAGTGAA CACGGGTCTG 
AGCATCATGA ACGATCTGGT GGTGCCCTGT CTTCCTCTGT TACTGGTGGA TGAGATGGAG 
GAGAAGGACA TGGTGGCTGT TGAAGACATG AGGAACCGAT GGTGCTCATA CCTGGGCCAG 
GAAATGGAGC CGAATCTACA GGAGAAGCTG ACCGACTTCC TCCCCAAACT GCTGGACTGC 
TCGACCGAGA TCAAAAGCTT TCACGAGCCG CCCAAACTGC CCTCTTACTC CACGCTGGAG 
CTGTTCGAGC GCTTCGGCAG GGTCATGACG TCCCTCACTA TGGGTCGAAC CCCTGCAGAT 
GGCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169214.1
CDS	207..3695
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTSQSSHPDSKLTSDPCPPPDPEQDDPTCPPPGPQRVVEVAIPNVGTFVIQSKEGGYDDEAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_005169157.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169157.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCTCAG CATGACCCAG AGTACATGGA GCAACAGTCG 
CCCAGCGGAG ACTCCAAACA CCTGCAGGAG GACACAGAGA GAGCCATCAG CAAGGCTGCA 
GCGGAGCATG ATGAGAAGAG TCCAGAAGCT CTGCTTAATA CATCTCAGTC ATCTCACCCT 
GACAGCAAGC TGACCTCTGA CCCCTGCCCT CCTCCTGACC CCGAGCAGGA CGACCCCACC 
TGCCCTCCAC CCGGCCCTCA GCGGGTGGTG GAGGTGGCCA TTCCTAATGT AGGGACCTTC 
GTCATTCAGT CGAAGGAGGG AGGTTATGAT GACGAGGCGA TGATGACCCC AAACATGCAG 
GGTATCATAA TGGCCATTGG CAAATCCAGG AGTGTTTATG ACAAGTGTGG GCCTGAAGCA 
GCGTTCTTCA AGGCGATGAA GGTTGAGTAC ACGCGTTTGC TGAGGCTTGC TCAGGAGGAC 
ACGCCGCCCG AGAGAGACTA CCGGCTACAG CATGTCATTG TGTTCTTTAT TCACAATGAG 
GCGCCCAAGA AGATTGTGGA GAGAACATTG CTCATGCAGT TTGCTGACAG AAACCTTGGC 
TTCGATGAGA GGTGTAAAAG CATCATGAAA GTGGCACGTG CCAAACTGGA GCTCATCAAG 
CCTGATGAGG TGAACTTGGA GGAGTACGAG ATGTGGCATC AGGACTACAG GATTTTCCGT 
GAGACGTCAG TGTTCCTGTT GATCGGCCTG GAATTATTCA TGAAGAAAAG TTTTGTGGAA 
GCGTTGATGT ATCTGATCTA CGCCTATCAG TACAACAAAG AGCTTCTGGC GAAAGGGCCG 
TACAGAGGCC ATGATGAGGA GCTGATCGCA CATTACCGCA GAGAGTGCCT GCTGAAGTTA 
AATGAATACG CTGCCGCTTT GTTCGAGACG GGCGAGGAGG CTAAAGTGAA CACGGGTCTG 
AGCATCATGA ACGATCTGGT GGTGCCCTGT CTTCCTCTGT TACTGGTGGA TGAGATGGAG 
GAGAAGGACA TGGTGGCTGT TGAAGACATG AGGAACCGAT GGTGCTCATA CCTGGGCCAG 
GAAATGGAGC CGAATCTACA GGAGAAGCTG ACCGACTTCC TCCCCAAACT GCTGGACTGC 
TCGACCGAGA TCAAAAGCTT TCACGAGCCG CCCAAACTGC CCTCTTACTC CACGCTGGAG 
CTGTTCGAGC GCTTCGGCAG GGTCATGACG TCCCTCACTA TGGGTCGAAC CCCTGCAGAT 
GGCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_005169157.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169214.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa16369
Clone ID Related Accession (Same CDS sequence)	XM_005169157.3 , XM_005169157.4
Accession Version	XM_005169157.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169157.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCTCAG CATGACCCAG AGTACATGGA GCAACAGTCG 
CCCAGCGGAG ACTCCAAACA CCTGCAGGAG GACACAGAGA GAGCCATCAG CAAGGCTGCA 
GCGGAGCATG ATGAGAAGAG TCCAGAAGCT CTGCTTAATA CATCTCAGTC ATCTCACCCT 
GACAGCAAGC TGACCTCTGA CCCCTGCCCT CCTCCTGACC CCGAGCAGGA CGACCCCACC 
TGCCCTCCAC CCGGCCCTCA GCGGGTGGTG GAGGTGGCCA TTCCTAATGT AGGGACCTTC 
GTCATTCAGT CGAAGGAGGG AGGTTATGAT GACGAGGCGA TGATGACCCC AAACATGCAG 
GGTATCATAA TGGCCATTGG CAAATCCAGG AGTGTTTATG ACAAGTGTGG GCCTGAAGCA 
GCGTTCTTCA AGGCGATGAA GGTTGAGTAC ACGCGTTTGC TGAGGCTTGC TCAGGAGGAC 
ACGCCGCCCG AGAGAGACTA CCGGCTACAG CATGTCATTG TGTTCTTTAT TCACAATGAG 
GCGCCCAAGA AGATTGTGGA GAGAACATTG CTCATGCAGT TTGCTGACAG AAACCTTGGC 
TTCGATGAGA GGTGTAAAAG CATCATGAAA GTGGCACGTG CCAAACTGGA GCTCATCAAG 
CCTGATGAGG TGAACTTGGA GGAGTACGAG ATGTGGCATC AGGACTACAG GATTTTCCGT 
GAGACGTCAG TGTTCCTGTT GATCGGCCTG GAATTATTCA TGAAGAAAAG TTTTGTGGAA 
GCGTTGATGT ATCTGATCTA CGCCTATCAG TACAACAAAG AGCTTCTGGC GAAAGGGCCG 
TACAGAGGCC ATGATGAGGA GCTGATCGCA CATTACCGCA GAGAGTGCCT GCTGAAGTTA 
AATGAATACG CTGCCGCTTT GTTCGAGACG GGCGAGGAGG CTAAAGTGAA CACGGGTCTG 
AGCATCATGA ACGATCTGGT GGTGCCCTGT CTTCCTCTGT TACTGGTGGA TGAGATGGAG 
GAGAAGGACA TGGTGGCTGT TGAAGACATG AGGAACCGAT GGTGCTCATA CCTGGGCCAG 
GAAATGGAGC CGAATCTACA GGAGAAGCTG ACCGACTTCC TCCCCAAACT GCTGGACTGC 
TCGACCGAGA TCAAAAGCTT TCACGAGCCG CCCAAACTGC CCTCTTACTC CACGCTGGAG 
CTGTTCGAGC GCTTCGGCAG GGTCATGACG TCCCTCACTA TGGGTCGAAC CCCTGCAGAT 
GGCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169214.1
CDS	209..3697
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTSQSSHPDSKLTSDPCPPPDPEQDDPTCPPPGPQRVVEVAIPNVGTFVIQSKEGGYDDEAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_005169157.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169157.4

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCTCAG CATGACCCAG AGTACATGGA GCAACAGTCG 
CCCAGCGGAG ACTCCAAACA CCTGCAGGAG GACACAGAGA GAGCCATCAG CAAGGCTGCA 
GCGGAGCATG ATGAGAAGAG TCCAGAAGCT CTGCTTAATA CATCTCAGTC ATCTCACCCT 
GACAGCAAGC TGACCTCTGA CCCCTGCCCT CCTCCTGACC CCGAGCAGGA CGACCCCACC 
TGCCCTCCAC CCGGCCCTCA GCGGGTGGTG GAGGTGGCCA TTCCTAATGT AGGGACCTTC 
GTCATTCAGT CGAAGGAGGG AGGTTATGAT GACGAGGCGA TGATGACCCC AAACATGCAG 
GGTATCATAA TGGCCATTGG CAAATCCAGG AGTGTTTATG ACAAGTGTGG GCCTGAAGCA 
GCGTTCTTCA AGGCGATGAA GGTTGAGTAC ACGCGTTTGC TGAGGCTTGC TCAGGAGGAC 
ACGCCGCCCG AGAGAGACTA CCGGCTACAG CATGTCATTG TGTTCTTTAT TCACAATGAG 
GCGCCCAAGA AGATTGTGGA GAGAACATTG CTCATGCAGT TTGCTGACAG AAACCTTGGC 
TTCGATGAGA GGTGTAAAAG CATCATGAAA GTGGCACGTG CCAAACTGGA GCTCATCAAG 
CCTGATGAGG TGAACTTGGA GGAGTACGAG ATGTGGCATC AGGACTACAG GATTTTCCGT 
GAGACGTCAG TGTTCCTGTT GATCGGCCTG GAATTATTCA TGAAGAAAAG TTTTGTGGAA 
GCGTTGATGT ATCTGATCTA CGCCTATCAG TACAACAAAG AGCTTCTGGC GAAAGGGCCG 
TACAGAGGCC ATGATGAGGA GCTGATCGCA CATTACCGCA GAGAGTGCCT GCTGAAGTTA 
AATGAATACG CTGCCGCTTT GTTCGAGACG GGCGAGGAGG CTAAAGTGAA CACGGGTCTG 
AGCATCATGA ACGATCTGGT GGTGCCCTGT CTTCCTCTGT TACTGGTGGA TGAGATGGAG 
GAGAAGGACA TGGTGGCTGT TGAAGACATG AGGAACCGAT GGTGCTCATA CCTGGGCCAG 
GAAATGGAGC CGAATCTACA GGAGAAGCTG ACCGACTTCC TCCCCAAACT GCTGGACTGC 
TCGACCGAGA TCAAAAGCTT TCACGAGCCG CCCAAACTGC CCTCTTACTC CACGCTGGAG 
CTGTTCGAGC GCTTCGGCAG GGTCATGACG TCCCTCACTA TGGGTCGAAC CCCTGCAGAT 
GGCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_005169161.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169218.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa16373
Clone ID Related Accession (Same CDS sequence)	XM_005169161.3 , XM_005169161.4
Accession Version	XM_005169161.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169161.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCAGCTCCT CAGCATGACC CAGAGTACAT GGAGCAACAG 
TCGCCCAGCG GAGACTCCAA ACACCTGCAG GAGGACACAG AGAGAGCCAT CAGCAAGGCT 
GCAGCGGAGC ATGATGAGAA GAGTCCAGAA GCTCTGCTTA ATACAGCGAT GAAGGTTGAG 
TACACGCGTT TGCTGAGGCT TGCTCAGGAG GACACGCCGC CCGAGAGAGA CTACCGGCTA 
CAGCATGTCA TTGTGTTCTT TATTCACAAT GAGGCGCCCA AGAAGATTGT GGAGAGAACA 
TTGCTCATGC AGTTTGCTGA CAGAAACCTT GGCTTCGATG AGAGGTGTAA AAGCATCATG 
AAAGTGGCAC GTGCCAAACT GGAGCTCATC AAGCCTGATG AGGTGAACTT GGAGGAGTAC 
GAGATGTGGC ATCAGGACTA CAGGATTTTC CGTGAGACGT CAGTGTTCCT GTTGATCGGC 
CTGGAATTAT TCATGAAGAA AAGTTTTGTG GAAGCGTTGA TGTATCTGAT CTACGCCTAT 
CAGTACAACA AAGAGCTTCT GGCGAAAGGG CCGTACAGAG GCCATGATGA GGAGCTGATC 
GCACATTACC GCAGAGAGTG CCTGCTGAAG TTAAATGAAT ACGCTGCCGC TTTGTTCGAG 
ACGGGCGAGG AGGCTAAAGT GAACACGGGT CTGAGCATCA TGAACGATCT GGTGGTGCCC 
TGTCTTCCTC TGTTACTGGT GGATGAGATG GAGGAGAAGG ACATGGTGGC TGTTGAAGAC 
ATGAGGAACC GATGGTGCTC ATACCTGGGC CAGGAAATGG AGCCGAATCT ACAGGAGAAG 
CTGACCGACT TCCTCCCCAA ACTGCTGGAC TGCTCGACCG AGATCAAAAG CTTTCACGAG 
CCGCCCAAAC TGCCCTCTTA CTCCACGCTG GAGCTGTTCG AGCGCTTCGG CAGGGTCATG 
ACGTCCCTCA CTATGGGTCG AACCCCTGCA GATGGCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169218.1
CDS	207..3428
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_005169161.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169161.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCAGCTCCT CAGCATGACC CAGAGTACAT GGAGCAACAG 
TCGCCCAGCG GAGACTCCAA ACACCTGCAG GAGGACACAG AGAGAGCCAT CAGCAAGGCT 
GCAGCGGAGC ATGATGAGAA GAGTCCAGAA GCTCTGCTTA ATACAGCGAT GAAGGTTGAG 
TACACGCGTT TGCTGAGGCT TGCTCAGGAG GACACGCCGC CCGAGAGAGA CTACCGGCTA 
CAGCATGTCA TTGTGTTCTT TATTCACAAT GAGGCGCCCA AGAAGATTGT GGAGAGAACA 
TTGCTCATGC AGTTTGCTGA CAGAAACCTT GGCTTCGATG AGAGGTGTAA AAGCATCATG 
AAAGTGGCAC GTGCCAAACT GGAGCTCATC AAGCCTGATG AGGTGAACTT GGAGGAGTAC 
GAGATGTGGC ATCAGGACTA CAGGATTTTC CGTGAGACGT CAGTGTTCCT GTTGATCGGC 
CTGGAATTAT TCATGAAGAA AAGTTTTGTG GAAGCGTTGA TGTATCTGAT CTACGCCTAT 
CAGTACAACA AAGAGCTTCT GGCGAAAGGG CCGTACAGAG GCCATGATGA GGAGCTGATC 
GCACATTACC GCAGAGAGTG CCTGCTGAAG TTAAATGAAT ACGCTGCCGC TTTGTTCGAG 
ACGGGCGAGG AGGCTAAAGT GAACACGGGT CTGAGCATCA TGAACGATCT GGTGGTGCCC 
TGTCTTCCTC TGTTACTGGT GGATGAGATG GAGGAGAAGG ACATGGTGGC TGTTGAAGAC 
ATGAGGAACC GATGGTGCTC ATACCTGGGC CAGGAAATGG AGCCGAATCT ACAGGAGAAG 
CTGACCGACT TCCTCCCCAA ACTGCTGGAC TGCTCGACCG AGATCAAAAG CTTTCACGAG 
CCGCCCAAAC TGCCCTCTTA CTCCACGCTG GAGCTGTTCG AGCGCTTCGG CAGGGTCATG 
ACGTCCCTCA CTATGGGTCG AACCCCTGCA GATGGCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_005169161.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169218.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa16373
Clone ID Related Accession (Same CDS sequence)	XM_005169161.3 , XM_005169161.4
Accession Version	XM_005169161.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169161.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCAGCTCCT CAGCATGACC CAGAGTACAT GGAGCAACAG 
TCGCCCAGCG GAGACTCCAA ACACCTGCAG GAGGACACAG AGAGAGCCAT CAGCAAGGCT 
GCAGCGGAGC ATGATGAGAA GAGTCCAGAA GCTCTGCTTA ATACAGCGAT GAAGGTTGAG 
TACACGCGTT TGCTGAGGCT TGCTCAGGAG GACACGCCGC CCGAGAGAGA CTACCGGCTA 
CAGCATGTCA TTGTGTTCTT TATTCACAAT GAGGCGCCCA AGAAGATTGT GGAGAGAACA 
TTGCTCATGC AGTTTGCTGA CAGAAACCTT GGCTTCGATG AGAGGTGTAA AAGCATCATG 
AAAGTGGCAC GTGCCAAACT GGAGCTCATC AAGCCTGATG AGGTGAACTT GGAGGAGTAC 
GAGATGTGGC ATCAGGACTA CAGGATTTTC CGTGAGACGT CAGTGTTCCT GTTGATCGGC 
CTGGAATTAT TCATGAAGAA AAGTTTTGTG GAAGCGTTGA TGTATCTGAT CTACGCCTAT 
CAGTACAACA AAGAGCTTCT GGCGAAAGGG CCGTACAGAG GCCATGATGA GGAGCTGATC 
GCACATTACC GCAGAGAGTG CCTGCTGAAG TTAAATGAAT ACGCTGCCGC TTTGTTCGAG 
ACGGGCGAGG AGGCTAAAGT GAACACGGGT CTGAGCATCA TGAACGATCT GGTGGTGCCC 
TGTCTTCCTC TGTTACTGGT GGATGAGATG GAGGAGAAGG ACATGGTGGC TGTTGAAGAC 
ATGAGGAACC GATGGTGCTC ATACCTGGGC CAGGAAATGG AGCCGAATCT ACAGGAGAAG 
CTGACCGACT TCCTCCCCAA ACTGCTGGAC TGCTCGACCG AGATCAAAAG CTTTCACGAG 
CCGCCCAAAC TGCCCTCTTA CTCCACGCTG GAGCTGTTCG AGCGCTTCGG CAGGGTCATG 
ACGTCCCTCA CTATGGGTCG AACCCCTGCA GATGGCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169218.1
CDS	208..3429
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_005169161.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169161.4

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCAGCTCCT CAGCATGACC CAGAGTACAT GGAGCAACAG 
TCGCCCAGCG GAGACTCCAA ACACCTGCAG GAGGACACAG AGAGAGCCAT CAGCAAGGCT 
GCAGCGGAGC ATGATGAGAA GAGTCCAGAA GCTCTGCTTA ATACAGCGAT GAAGGTTGAG 
TACACGCGTT TGCTGAGGCT TGCTCAGGAG GACACGCCGC CCGAGAGAGA CTACCGGCTA 
CAGCATGTCA TTGTGTTCTT TATTCACAAT GAGGCGCCCA AGAAGATTGT GGAGAGAACA 
TTGCTCATGC AGTTTGCTGA CAGAAACCTT GGCTTCGATG AGAGGTGTAA AAGCATCATG 
AAAGTGGCAC GTGCCAAACT GGAGCTCATC AAGCCTGATG AGGTGAACTT GGAGGAGTAC 
GAGATGTGGC ATCAGGACTA CAGGATTTTC CGTGAGACGT CAGTGTTCCT GTTGATCGGC 
CTGGAATTAT TCATGAAGAA AAGTTTTGTG GAAGCGTTGA TGTATCTGAT CTACGCCTAT 
CAGTACAACA AAGAGCTTCT GGCGAAAGGG CCGTACAGAG GCCATGATGA GGAGCTGATC 
GCACATTACC GCAGAGAGTG CCTGCTGAAG TTAAATGAAT ACGCTGCCGC TTTGTTCGAG 
ACGGGCGAGG AGGCTAAAGT GAACACGGGT CTGAGCATCA TGAACGATCT GGTGGTGCCC 
TGTCTTCCTC TGTTACTGGT GGATGAGATG GAGGAGAAGG ACATGGTGGC TGTTGAAGAC 
ATGAGGAACC GATGGTGCTC ATACCTGGGC CAGGAAATGG AGCCGAATCT ACAGGAGAAG 
CTGACCGACT TCCTCCCCAA ACTGCTGGAC TGCTCGACCG AGATCAAAAG CTTTCACGAG 
CCGCCCAAAC TGCCCTCTTA CTCCACGCTG GAGCTGTTCG AGCGCTTCGG CAGGGTCATG 
ACGTCCCTCA CTATGGGTCG AACCCCTGCA GATGGCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_009305557.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303832.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40921
Clone ID Related Accession (Same CDS sequence)	XM_009305557.2 , XM_009305557.3
Accession Version	XM_009305557.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCATCA GCAGCTCCTC AGCATGACCC AGAGTACATG 
GAGCAACAGT CGCCCAGCGG AGACTCCAAA CACCTGCAGG AGGACACAGA GAGAGCCATC 
AGCAAGGCTG CAGCGGAGCA TGATGAGAAG AGTCCAGAAG CTCTGCTTAA TACATCTCAG 
TCATCTCACC CTGACAGCAA GCTGACCTCT GACCCCTGCC CTCCTCCTGA CCCCGAGCAG 
GACGACCCCA CCTGCCCTCC ACCCGGCCCT CAGCGGGTGG TGGAGGTGGC CATTCCTAAT 
GTAGGGACCT TCGTCATTCA GTCGAAGGAG GGAGGTTATG ATGACGAGGC GATGATGACC 
CCAAACATGC AGGGTATCAT AATGGCCATT GGCAAATCCA GGAGTGTTTA TGACAAGTGT 
GGGCCTGAAG CAGCGTTCTT CAAGGCGATG AAGGTTGAGT ACACGCGTTT GCTGAGGCTT 
GCTCAGGAGG ACACGCCGCC CGAGAGAGAC TACCGGCTAC AGCATGTCAT TGTGTTCTTT 
ATTCACAATG AGGCGCCCAA GAAGATTGTG GAGAGAACAT TGCTCATGCA GTTTGCTGAC 
AGAAACCTTG GCTTCGATGA GAGGTGTAAA AGCATCATGA AAGTGGCACG TGCCAAACTG 
GAGCTCATCA AGCCTGATGA GGTGAACTTG GAGGAGTACG AGATGTGGCA TCAGGACTAC 
AGGATTTTCC GTGAGACGTC AGTGTTCCTG TTGATCGGCC TGGAATTATT CATGAAGAAA 
AGTTTTGTGG AAGCGTTGAT GTATCTGATC TACGCCTATC AGTACAACAA AGAGCTTCTG 
GCGAAAGGGC CGTACAGAGG CCATGATGAG GAGCTGATCG CACATTACCG CAGAGAGTGC 
CTGCTGAAGT TAAATGAATA CGCTGCCGCT TTGTTCGAGA CGGGCGAGGA GGCTAAAGTG 
AACACGGGTC TGAGCATCAT GAACGATCTG GTGGTGCCCT GTCTTCCTCT GTTACTGGTG 
GATGAGATGG AGGAGAAGGA CATGGTGGCT GTTGAAGACA TGAGGAACCG ATGGTGCTCA 
TACCTGGGCC AGGAAATGGA GCCGAATCTA CAGGAGAAGC TGACCGACTT CCTCCCCAAA 
CTGCTGGACT GCTCGACCGA GATCAAAAGC TTTCACGAGC CGCCCAAACT GCCCTCTTAC 
TCCACGCTGG AGCTGTTCGA GCGCTTCGGC AGGGTCATGA CGTCCCTCAC TATGGGTCGA 
ACCCCTGCAG ATGGCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303832.1
CDS	207..3707
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPSAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTSQSSHPDSKLTSDPCPPPDPEQDDPTCPPPGPQRVVEVAIPNVGTFVIQSKEGGYDDEAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_009305557.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305557.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCATCA GCAGCTCCTC AGCATGACCC AGAGTACATG 
GAGCAACAGT CGCCCAGCGG AGACTCCAAA CACCTGCAGG AGGACACAGA GAGAGCCATC 
AGCAAGGCTG CAGCGGAGCA TGATGAGAAG AGTCCAGAAG CTCTGCTTAA TACATCTCAG 
TCATCTCACC CTGACAGCAA GCTGACCTCT GACCCCTGCC CTCCTCCTGA CCCCGAGCAG 
GACGACCCCA CCTGCCCTCC ACCCGGCCCT CAGCGGGTGG TGGAGGTGGC CATTCCTAAT 
GTAGGGACCT TCGTCATTCA GTCGAAGGAG GGAGGTTATG ATGACGAGGC GATGATGACC 
CCAAACATGC AGGGTATCAT AATGGCCATT GGCAAATCCA GGAGTGTTTA TGACAAGTGT 
GGGCCTGAAG CAGCGTTCTT CAAGGCGATG AAGGTTGAGT ACACGCGTTT GCTGAGGCTT 
GCTCAGGAGG ACACGCCGCC CGAGAGAGAC TACCGGCTAC AGCATGTCAT TGTGTTCTTT 
ATTCACAATG AGGCGCCCAA GAAGATTGTG GAGAGAACAT TGCTCATGCA GTTTGCTGAC 
AGAAACCTTG GCTTCGATGA GAGGTGTAAA AGCATCATGA AAGTGGCACG TGCCAAACTG 
GAGCTCATCA AGCCTGATGA GGTGAACTTG GAGGAGTACG AGATGTGGCA TCAGGACTAC 
AGGATTTTCC GTGAGACGTC AGTGTTCCTG TTGATCGGCC TGGAATTATT CATGAAGAAA 
AGTTTTGTGG AAGCGTTGAT GTATCTGATC TACGCCTATC AGTACAACAA AGAGCTTCTG 
GCGAAAGGGC CGTACAGAGG CCATGATGAG GAGCTGATCG CACATTACCG CAGAGAGTGC 
CTGCTGAAGT TAAATGAATA CGCTGCCGCT TTGTTCGAGA CGGGCGAGGA GGCTAAAGTG 
AACACGGGTC TGAGCATCAT GAACGATCTG GTGGTGCCCT GTCTTCCTCT GTTACTGGTG 
GATGAGATGG AGGAGAAGGA CATGGTGGCT GTTGAAGACA TGAGGAACCG ATGGTGCTCA 
TACCTGGGCC AGGAAATGGA GCCGAATCTA CAGGAGAAGC TGACCGACTT CCTCCCCAAA 
CTGCTGGACT GCTCGACCGA GATCAAAAGC TTTCACGAGC CGCCCAAACT GCCCTCTTAC 
TCCACGCTGG AGCTGTTCGA GCGCTTCGGC AGGGTCATGA CGTCCCTCAC TATGGGTCGA 
ACCCCTGCAG ATGGCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_009305557.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303832.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40921
Clone ID Related Accession (Same CDS sequence)	XM_009305557.2 , XM_009305557.3
Accession Version	XM_009305557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305557.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCATCA GCAGCTCCTC AGCATGACCC AGAGTACATG 
GAGCAACAGT CGCCCAGCGG AGACTCCAAA CACCTGCAGG AGGACACAGA GAGAGCCATC 
AGCAAGGCTG CAGCGGAGCA TGATGAGAAG AGTCCAGAAG CTCTGCTTAA TACATCTCAG 
TCATCTCACC CTGACAGCAA GCTGACCTCT GACCCCTGCC CTCCTCCTGA CCCCGAGCAG 
GACGACCCCA CCTGCCCTCC ACCCGGCCCT CAGCGGGTGG TGGAGGTGGC CATTCCTAAT 
GTAGGGACCT TCGTCATTCA GTCGAAGGAG GGAGGTTATG ATGACGAGGC GATGATGACC 
CCAAACATGC AGGGTATCAT AATGGCCATT GGCAAATCCA GGAGTGTTTA TGACAAGTGT 
GGGCCTGAAG CAGCGTTCTT CAAGGCGATG AAGGTTGAGT ACACGCGTTT GCTGAGGCTT 
GCTCAGGAGG ACACGCCGCC CGAGAGAGAC TACCGGCTAC AGCATGTCAT TGTGTTCTTT 
ATTCACAATG AGGCGCCCAA GAAGATTGTG GAGAGAACAT TGCTCATGCA GTTTGCTGAC 
AGAAACCTTG GCTTCGATGA GAGGTGTAAA AGCATCATGA AAGTGGCACG TGCCAAACTG 
GAGCTCATCA AGCCTGATGA GGTGAACTTG GAGGAGTACG AGATGTGGCA TCAGGACTAC 
AGGATTTTCC GTGAGACGTC AGTGTTCCTG TTGATCGGCC TGGAATTATT CATGAAGAAA 
AGTTTTGTGG AAGCGTTGAT GTATCTGATC TACGCCTATC AGTACAACAA AGAGCTTCTG 
GCGAAAGGGC CGTACAGAGG CCATGATGAG GAGCTGATCG CACATTACCG CAGAGAGTGC 
CTGCTGAAGT TAAATGAATA CGCTGCCGCT TTGTTCGAGA CGGGCGAGGA GGCTAAAGTG 
AACACGGGTC TGAGCATCAT GAACGATCTG GTGGTGCCCT GTCTTCCTCT GTTACTGGTG 
GATGAGATGG AGGAGAAGGA CATGGTGGCT GTTGAAGACA TGAGGAACCG ATGGTGCTCA 
TACCTGGGCC AGGAAATGGA GCCGAATCTA CAGGAGAAGC TGACCGACTT CCTCCCCAAA 
CTGCTGGACT GCTCGACCGA GATCAAAAGC TTTCACGAGC CGCCCAAACT GCCCTCTTAC 
TCCACGCTGG AGCTGTTCGA GCGCTTCGGC AGGGTCATGA CGTCCCTCAC TATGGGTCGA 
ACCCCTGCAG ATGGCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303832.1
CDS	210..3710
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPSAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTSQSSHPDSKLTSDPCPPPDPEQDDPTCPPPGPQRVVEVAIPNVGTFVIQSKEGGYDDEAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_009305557.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305557.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCATCA GCAGCTCCTC AGCATGACCC AGAGTACATG 
GAGCAACAGT CGCCCAGCGG AGACTCCAAA CACCTGCAGG AGGACACAGA GAGAGCCATC 
AGCAAGGCTG CAGCGGAGCA TGATGAGAAG AGTCCAGAAG CTCTGCTTAA TACATCTCAG 
TCATCTCACC CTGACAGCAA GCTGACCTCT GACCCCTGCC CTCCTCCTGA CCCCGAGCAG 
GACGACCCCA CCTGCCCTCC ACCCGGCCCT CAGCGGGTGG TGGAGGTGGC CATTCCTAAT 
GTAGGGACCT TCGTCATTCA GTCGAAGGAG GGAGGTTATG ATGACGAGGC GATGATGACC 
CCAAACATGC AGGGTATCAT AATGGCCATT GGCAAATCCA GGAGTGTTTA TGACAAGTGT 
GGGCCTGAAG CAGCGTTCTT CAAGGCGATG AAGGTTGAGT ACACGCGTTT GCTGAGGCTT 
GCTCAGGAGG ACACGCCGCC CGAGAGAGAC TACCGGCTAC AGCATGTCAT TGTGTTCTTT 
ATTCACAATG AGGCGCCCAA GAAGATTGTG GAGAGAACAT TGCTCATGCA GTTTGCTGAC 
AGAAACCTTG GCTTCGATGA GAGGTGTAAA AGCATCATGA AAGTGGCACG TGCCAAACTG 
GAGCTCATCA AGCCTGATGA GGTGAACTTG GAGGAGTACG AGATGTGGCA TCAGGACTAC 
AGGATTTTCC GTGAGACGTC AGTGTTCCTG TTGATCGGCC TGGAATTATT CATGAAGAAA 
AGTTTTGTGG AAGCGTTGAT GTATCTGATC TACGCCTATC AGTACAACAA AGAGCTTCTG 
GCGAAAGGGC CGTACAGAGG CCATGATGAG GAGCTGATCG CACATTACCG CAGAGAGTGC 
CTGCTGAAGT TAAATGAATA CGCTGCCGCT TTGTTCGAGA CGGGCGAGGA GGCTAAAGTG 
AACACGGGTC TGAGCATCAT GAACGATCTG GTGGTGCCCT GTCTTCCTCT GTTACTGGTG 
GATGAGATGG AGGAGAAGGA CATGGTGGCT GTTGAAGACA TGAGGAACCG ATGGTGCTCA 
TACCTGGGCC AGGAAATGGA GCCGAATCTA CAGGAGAAGC TGACCGACTT CCTCCCCAAA 
CTGCTGGACT GCTCGACCGA GATCAAAAGC TTTCACGAGC CGCCCAAACT GCCCTCTTAC 
TCCACGCTGG AGCTGTTCGA GCGCTTCGGC AGGGTCATGA CGTCCCTCAC TATGGGTCGA 
ACCCCTGCAG ATGGCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_009305558.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303833.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa40922
Clone ID Related Accession (Same CDS sequence)	XM_009305558.2 , XM_009305558.3
Accession Version	XM_009305558.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGGAT 
GTGAGCGAGT TCACCCATAA ACTTCTGGAC TGGCTGGAGG ACGCATTTCA GATTAAAGCA 
GAAGAGGACA GGGAGGGAGA GAAACCCAAG AACCCGATGG TTGAGCTGTT TTATGGACGT 
TTCCTGGCTG TGGGCGTCCT GGAGGGAAAG AAATTTGAAA ACACTGAGAT GTTTGGCCAG 
TATCCTCTCC AGGTGAACGG CTTCAAAGAT CTACACGAGT GTTTAGAGGC CGCCATGATA 
GAGGGAGAAA TCGAGTCTTT GCATTCTGCA GAAAACTCCG CCAAATCAGG CCAAGAGCAT 
TGGTTCACAG AGCTTCCACC CGTTTTGACC TTTGAACTGT CCAGGTTTGA GTTTAATCAA 
GCCTTAGGCC GACCTGAGAA GATTCACAAT AAGCTCGAGT TTCCTTCTAT GCTGTACATG 
GACAGGTACA TGGACAGGAA CCGAGAGATC ACACGGATAA AGCGAGAGGA GATCAGAAGA 
CTAAAGGAGC ACCTCACCGT CCTCCAGCAG CGACTCGAGA GATATCTGAG TTATGGCTCG 
GGGCCCAAGC GCTTTCCTCT GGCTGATGTT CTCCAATATG CCATGGAGTT TGCGTCCAGT 
AAACCAGTGT GCACGTCTCC AGTGGAGGAC ATTGATACAA CAGCACCACC TGGTGGCACA 
ATTGCGCAGC TGCCACCGCC AGCAAGCGCA GGGGAACAGC CGGATGCGTG CGTCTCAGCC 
GAGGGCTCTG GTTCTGGTCT GCAGGCTTCG CAACAGCAGC AGCAGAGGGT TTCCATCCAT 
AAACCGTTCA CACAGTCCCG AGTGCCGCCG GATCTGCCCA TGCATCCCGC TCCTCGACAC 
ATCACTGAGG AGGAGCTGCG GGTGCTGGAG AGCTGCCTGC ACCGCTGGAG GAGCGAGGTG 
GAGAACGACA CCAGAGATCT GCAGGCCAGC ATATCCAGAA TCCACAGAAC TATCGAGCTC 
ATGTATTCTG ACAAGTCTAT GATGCAGGTG CCGTATCGAC TACATGCTGT TCTGGTCCAC 
GAGGGTCAGG CGAATGCTGG TCACTACTGG GCCTACATTT ATGACCGACA TCACCAGCGC 
TGGATGAAAT ACAACGACAT TGCAGTGACA AAGTCATCAT GGGAAGAGCT AGTGCGGGAC 
TCGTTTGGTG GATATCGTAA CGCCAGCGCA TACTGCCTCA TGTACATTGA TGATAAGAAA 
CCCTTCCTTA TTCAAGAAGA GTTTGATAAA GAGACGGGAC AGATGCTCAG TGGTTTGGAC 
AAGCTTCCTC CTGACCTGAA GGTGTTTGTG GAGGCAGATA ACAAGCTCTT CGAGAGGGAG 
ATGGAGGAGT GGGATGCTTT ACAGGCCCGC AAACTCCAGC AGGAAAAGTT GGCTCTGGCT 
GCTGCTGCTG CGGCTGCTGC CGCCGCTAGT GTGGCCGTCC AGCCCATGAG CACTGAACCC 
TGCTCTCCAG ACAACACAGC TCCATCAGCA GCTCCTCAGC ATGACCCAGA GTACATGGAG 
CAACAGTCGC CCAGCGGAGA CTCCAAACAC CTGCAGGAGG ACACAGAGAG AGCCATCAGC 
AAGGCTGCAG CGGAGCATGA TGAGAAGAGT CCAGAAGCTC TGCTTAATAC ATCTCAGTCA 
TCTCACCCTG ACAGCAAGCT GACCTCTGAC CCCTGCCCTC CTCCTGACCC CGAGCAGGAC 
GACCCCACCT GCCCTCCACC CGGCCCTCAG CGGGTGGTGG AGGTGGCCAT TCCTAATGTA 
GGGACCTTCG TCATTCAGTC GAAGGAGGGA GGTTATGATG ACGAGGCGAT GATGACCCCA 
AACATGCAGG GTATCATAAT GGCCATTGGC AAATCCAGGA GTGTTTATGA CAAGTGTGGG 
CCTGAAGCAG CGTTCTTCAA GGCGATGAAG GTTGAGTACA CGCGTTTGCT GAGGCTTGCT 
CAGGAGGACA CGCCGCCCGA GAGAGACTAC CGGCTACAGC ATGTCATTGT GTTCTTTATT 
CACAATGAGG CGCCCAAGAA GATTGTGGAG AGAACATTGC TCATGCAGTT TGCTGACAGA 
AACCTTGGCT TCGATGAGAG GTGTAAAAGC ATCATGAAAG TGGCACGTGC CAAACTGGAG 
CTCATCAAGC CTGATGAGGT GAACTTGGAG GAGTACGAGA TGTGGCATCA GGACTACAGG 
ATTTTCCGTG AGACGTCAGT GTTCCTGTTG ATCGGCCTGG AATTATTCAT GAAGAAAAGT 
TTTGTGGAAG CGTTGATGTA TCTGATCTAC GCCTATCAGT ACAACAAAGA GCTTCTGGCG 
AAAGGGCCGT ACAGAGGCCA TGATGAGGAG CTGATCGCAC ATTACCGCAG AGAGTGCCTG 
CTGAAGTTAA ATGAATACGC TGCCGCTTTG TTCGAGACGG GCGAGGAGGC TAAAGTGAAC 
ACGGGTCTGA GCATCATGAA CGATCTGGTG GTGCCCTGTC TTCCTCTGTT ACTGGTGGAT 
GAGATGGAGG AGAAGGACAT GGTGGCTGTT GAAGACATGA GGAACCGATG GTGCTCATAC 
CTGGGCCAGG AAATGGAGCC GAATCTACAG GAGAAGCTGA CCGACTTCCT CCCCAAACTG 
CTGGACTGCT CGACCGAGAT CAAAAGCTTT CACGAGCCGC CCAAACTGCC CTCTTACTCC 
ACGCTGGAGC TGTTCGAGCG CTTCGGCAGG GTCATGACGT CCCTCACTAT GGGTCGAACC 
CCTGCAGATG GCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303833.1
CDS	207..3704
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPSAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTSQSSHPDSKLTSDPCPPPDPEQDDPTCPPPGPQRVVEVAIPNVGTFVIQSKEGGYDDEAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_009305558.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305558.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGGAT 
GTGAGCGAGT TCACCCATAA ACTTCTGGAC TGGCTGGAGG ACGCATTTCA GATTAAAGCA 
GAAGAGGACA GGGAGGGAGA GAAACCCAAG AACCCGATGG TTGAGCTGTT TTATGGACGT 
TTCCTGGCTG TGGGCGTCCT GGAGGGAAAG AAATTTGAAA ACACTGAGAT GTTTGGCCAG 
TATCCTCTCC AGGTGAACGG CTTCAAAGAT CTACACGAGT GTTTAGAGGC CGCCATGATA 
GAGGGAGAAA TCGAGTCTTT GCATTCTGCA GAAAACTCCG CCAAATCAGG CCAAGAGCAT 
TGGTTCACAG AGCTTCCACC CGTTTTGACC TTTGAACTGT CCAGGTTTGA GTTTAATCAA 
GCCTTAGGCC GACCTGAGAA GATTCACAAT AAGCTCGAGT TTCCTTCTAT GCTGTACATG 
GACAGGTACA TGGACAGGAA CCGAGAGATC ACACGGATAA AGCGAGAGGA GATCAGAAGA 
CTAAAGGAGC ACCTCACCGT CCTCCAGCAG CGACTCGAGA GATATCTGAG TTATGGCTCG 
GGGCCCAAGC GCTTTCCTCT GGCTGATGTT CTCCAATATG CCATGGAGTT TGCGTCCAGT 
AAACCAGTGT GCACGTCTCC AGTGGAGGAC ATTGATACAA CAGCACCACC TGGTGGCACA 
ATTGCGCAGC TGCCACCGCC AGCAAGCGCA GGGGAACAGC CGGATGCGTG CGTCTCAGCC 
GAGGGCTCTG GTTCTGGTCT GCAGGCTTCG CAACAGCAGC AGCAGAGGGT TTCCATCCAT 
AAACCGTTCA CACAGTCCCG AGTGCCGCCG GATCTGCCCA TGCATCCCGC TCCTCGACAC 
ATCACTGAGG AGGAGCTGCG GGTGCTGGAG AGCTGCCTGC ACCGCTGGAG GAGCGAGGTG 
GAGAACGACA CCAGAGATCT GCAGGCCAGC ATATCCAGAA TCCACAGAAC TATCGAGCTC 
ATGTATTCTG ACAAGTCTAT GATGCAGGTG CCGTATCGAC TACATGCTGT TCTGGTCCAC 
GAGGGTCAGG CGAATGCTGG TCACTACTGG GCCTACATTT ATGACCGACA TCACCAGCGC 
TGGATGAAAT ACAACGACAT TGCAGTGACA AAGTCATCAT GGGAAGAGCT AGTGCGGGAC 
TCGTTTGGTG GATATCGTAA CGCCAGCGCA TACTGCCTCA TGTACATTGA TGATAAGAAA 
CCCTTCCTTA TTCAAGAAGA GTTTGATAAA GAGACGGGAC AGATGCTCAG TGGTTTGGAC 
AAGCTTCCTC CTGACCTGAA GGTGTTTGTG GAGGCAGATA ACAAGCTCTT CGAGAGGGAG 
ATGGAGGAGT GGGATGCTTT ACAGGCCCGC AAACTCCAGC AGGAAAAGTT GGCTCTGGCT 
GCTGCTGCTG CGGCTGCTGC CGCCGCTAGT GTGGCCGTCC AGCCCATGAG CACTGAACCC 
TGCTCTCCAG ACAACACAGC TCCATCAGCA GCTCCTCAGC ATGACCCAGA GTACATGGAG 
CAACAGTCGC CCAGCGGAGA CTCCAAACAC CTGCAGGAGG ACACAGAGAG AGCCATCAGC 
AAGGCTGCAG CGGAGCATGA TGAGAAGAGT CCAGAAGCTC TGCTTAATAC ATCTCAGTCA 
TCTCACCCTG ACAGCAAGCT GACCTCTGAC CCCTGCCCTC CTCCTGACCC CGAGCAGGAC 
GACCCCACCT GCCCTCCACC CGGCCCTCAG CGGGTGGTGG AGGTGGCCAT TCCTAATGTA 
GGGACCTTCG TCATTCAGTC GAAGGAGGGA GGTTATGATG ACGAGGCGAT GATGACCCCA 
AACATGCAGG GTATCATAAT GGCCATTGGC AAATCCAGGA GTGTTTATGA CAAGTGTGGG 
CCTGAAGCAG CGTTCTTCAA GGCGATGAAG GTTGAGTACA CGCGTTTGCT GAGGCTTGCT 
CAGGAGGACA CGCCGCCCGA GAGAGACTAC CGGCTACAGC ATGTCATTGT GTTCTTTATT 
CACAATGAGG CGCCCAAGAA GATTGTGGAG AGAACATTGC TCATGCAGTT TGCTGACAGA 
AACCTTGGCT TCGATGAGAG GTGTAAAAGC ATCATGAAAG TGGCACGTGC CAAACTGGAG 
CTCATCAAGC CTGATGAGGT GAACTTGGAG GAGTACGAGA TGTGGCATCA GGACTACAGG 
ATTTTCCGTG AGACGTCAGT GTTCCTGTTG ATCGGCCTGG AATTATTCAT GAAGAAAAGT 
TTTGTGGAAG CGTTGATGTA TCTGATCTAC GCCTATCAGT ACAACAAAGA GCTTCTGGCG 
AAAGGGCCGT ACAGAGGCCA TGATGAGGAG CTGATCGCAC ATTACCGCAG AGAGTGCCTG 
CTGAAGTTAA ATGAATACGC TGCCGCTTTG TTCGAGACGG GCGAGGAGGC TAAAGTGAAC 
ACGGGTCTGA GCATCATGAA CGATCTGGTG GTGCCCTGTC TTCCTCTGTT ACTGGTGGAT 
GAGATGGAGG AGAAGGACAT GGTGGCTGTT GAAGACATGA GGAACCGATG GTGCTCATAC 
CTGGGCCAGG AAATGGAGCC GAATCTACAG GAGAAGCTGA CCGACTTCCT CCCCAAACTG 
CTGGACTGCT CGACCGAGAT CAAAAGCTTT CACGAGCCGC CCAAACTGCC CTCTTACTCC 
ACGCTGGAGC TGTTCGAGCG CTTCGGCAGG GTCATGACGT CCCTCACTAT GGGTCGAACC 
CCTGCAGATG GCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_009305558.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303833.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40922
Clone ID Related Accession (Same CDS sequence)	XM_009305558.2 , XM_009305558.3
Accession Version	XM_009305558.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305558.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGGAT 
GTGAGCGAGT TCACCCATAA ACTTCTGGAC TGGCTGGAGG ACGCATTTCA GATTAAAGCA 
GAAGAGGACA GGGAGGGAGA GAAACCCAAG AACCCGATGG TTGAGCTGTT TTATGGACGT 
TTCCTGGCTG TGGGCGTCCT GGAGGGAAAG AAATTTGAAA ACACTGAGAT GTTTGGCCAG 
TATCCTCTCC AGGTGAACGG CTTCAAAGAT CTACACGAGT GTTTAGAGGC CGCCATGATA 
GAGGGAGAAA TCGAGTCTTT GCATTCTGCA GAAAACTCCG CCAAATCAGG CCAAGAGCAT 
TGGTTCACAG AGCTTCCACC CGTTTTGACC TTTGAACTGT CCAGGTTTGA GTTTAATCAA 
GCCTTAGGCC GACCTGAGAA GATTCACAAT AAGCTCGAGT TTCCTTCTAT GCTGTACATG 
GACAGGTACA TGGACAGGAA CCGAGAGATC ACACGGATAA AGCGAGAGGA GATCAGAAGA 
CTAAAGGAGC ACCTCACCGT CCTCCAGCAG CGACTCGAGA GATATCTGAG TTATGGCTCG 
GGGCCCAAGC GCTTTCCTCT GGCTGATGTT CTCCAATATG CCATGGAGTT TGCGTCCAGT 
AAACCAGTGT GCACGTCTCC AGTGGAGGAC ATTGATACAA CAGCACCACC TGGTGGCACA 
ATTGCGCAGC TGCCACCGCC AGCAAGCGCA GGGGAACAGC CGGATGCGTG CGTCTCAGCC 
GAGGGCTCTG GTTCTGGTCT GCAGGCTTCG CAACAGCAGC AGCAGAGGGT TTCCATCCAT 
AAACCGTTCA CACAGTCCCG AGTGCCGCCG GATCTGCCCA TGCATCCCGC TCCTCGACAC 
ATCACTGAGG AGGAGCTGCG GGTGCTGGAG AGCTGCCTGC ACCGCTGGAG GAGCGAGGTG 
GAGAACGACA CCAGAGATCT GCAGGCCAGC ATATCCAGAA TCCACAGAAC TATCGAGCTC 
ATGTATTCTG ACAAGTCTAT GATGCAGGTG CCGTATCGAC TACATGCTGT TCTGGTCCAC 
GAGGGTCAGG CGAATGCTGG TCACTACTGG GCCTACATTT ATGACCGACA TCACCAGCGC 
TGGATGAAAT ACAACGACAT TGCAGTGACA AAGTCATCAT GGGAAGAGCT AGTGCGGGAC 
TCGTTTGGTG GATATCGTAA CGCCAGCGCA TACTGCCTCA TGTACATTGA TGATAAGAAA 
CCCTTCCTTA TTCAAGAAGA GTTTGATAAA GAGACGGGAC AGATGCTCAG TGGTTTGGAC 
AAGCTTCCTC CTGACCTGAA GGTGTTTGTG GAGGCAGATA ACAAGCTCTT CGAGAGGGAG 
ATGGAGGAGT GGGATGCTTT ACAGGCCCGC AAACTCCAGC AGGAAAAGTT GGCTCTGGCT 
GCTGCTGCTG CGGCTGCTGC CGCCGCTAGT GTGGCCGTCC AGCCCATGAG CACTGAACCC 
TGCTCTCCAG ACAACACAGC TCCATCAGCA GCTCCTCAGC ATGACCCAGA GTACATGGAG 
CAACAGTCGC CCAGCGGAGA CTCCAAACAC CTGCAGGAGG ACACAGAGAG AGCCATCAGC 
AAGGCTGCAG CGGAGCATGA TGAGAAGAGT CCAGAAGCTC TGCTTAATAC ATCTCAGTCA 
TCTCACCCTG ACAGCAAGCT GACCTCTGAC CCCTGCCCTC CTCCTGACCC CGAGCAGGAC 
GACCCCACCT GCCCTCCACC CGGCCCTCAG CGGGTGGTGG AGGTGGCCAT TCCTAATGTA 
GGGACCTTCG TCATTCAGTC GAAGGAGGGA GGTTATGATG ACGAGGCGAT GATGACCCCA 
AACATGCAGG GTATCATAAT GGCCATTGGC AAATCCAGGA GTGTTTATGA CAAGTGTGGG 
CCTGAAGCAG CGTTCTTCAA GGCGATGAAG GTTGAGTACA CGCGTTTGCT GAGGCTTGCT 
CAGGAGGACA CGCCGCCCGA GAGAGACTAC CGGCTACAGC ATGTCATTGT GTTCTTTATT 
CACAATGAGG CGCCCAAGAA GATTGTGGAG AGAACATTGC TCATGCAGTT TGCTGACAGA 
AACCTTGGCT TCGATGAGAG GTGTAAAAGC ATCATGAAAG TGGCACGTGC CAAACTGGAG 
CTCATCAAGC CTGATGAGGT GAACTTGGAG GAGTACGAGA TGTGGCATCA GGACTACAGG 
ATTTTCCGTG AGACGTCAGT GTTCCTGTTG ATCGGCCTGG AATTATTCAT GAAGAAAAGT 
TTTGTGGAAG CGTTGATGTA TCTGATCTAC GCCTATCAGT ACAACAAAGA GCTTCTGGCG 
AAAGGGCCGT ACAGAGGCCA TGATGAGGAG CTGATCGCAC ATTACCGCAG AGAGTGCCTG 
CTGAAGTTAA ATGAATACGC TGCCGCTTTG TTCGAGACGG GCGAGGAGGC TAAAGTGAAC 
ACGGGTCTGA GCATCATGAA CGATCTGGTG GTGCCCTGTC TTCCTCTGTT ACTGGTGGAT 
GAGATGGAGG AGAAGGACAT GGTGGCTGTT GAAGACATGA GGAACCGATG GTGCTCATAC 
CTGGGCCAGG AAATGGAGCC GAATCTACAG GAGAAGCTGA CCGACTTCCT CCCCAAACTG 
CTGGACTGCT CGACCGAGAT CAAAAGCTTT CACGAGCCGC CCAAACTGCC CTCTTACTCC 
ACGCTGGAGC TGTTCGAGCG CTTCGGCAGG GTCATGACGT CCCTCACTAT GGGTCGAACC 
CCTGCAGATG GCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303833.1
CDS	210..3707
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPSAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTSQSSHPDSKLTSDPCPPPDPEQDDPTCPPPGPQRVVEVAIPNVGTFVIQSKEGGYDDEAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_009305558.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305558.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGGAT 
GTGAGCGAGT TCACCCATAA ACTTCTGGAC TGGCTGGAGG ACGCATTTCA GATTAAAGCA 
GAAGAGGACA GGGAGGGAGA GAAACCCAAG AACCCGATGG TTGAGCTGTT TTATGGACGT 
TTCCTGGCTG TGGGCGTCCT GGAGGGAAAG AAATTTGAAA ACACTGAGAT GTTTGGCCAG 
TATCCTCTCC AGGTGAACGG CTTCAAAGAT CTACACGAGT GTTTAGAGGC CGCCATGATA 
GAGGGAGAAA TCGAGTCTTT GCATTCTGCA GAAAACTCCG CCAAATCAGG CCAAGAGCAT 
TGGTTCACAG AGCTTCCACC CGTTTTGACC TTTGAACTGT CCAGGTTTGA GTTTAATCAA 
GCCTTAGGCC GACCTGAGAA GATTCACAAT AAGCTCGAGT TTCCTTCTAT GCTGTACATG 
GACAGGTACA TGGACAGGAA CCGAGAGATC ACACGGATAA AGCGAGAGGA GATCAGAAGA 
CTAAAGGAGC ACCTCACCGT CCTCCAGCAG CGACTCGAGA GATATCTGAG TTATGGCTCG 
GGGCCCAAGC GCTTTCCTCT GGCTGATGTT CTCCAATATG CCATGGAGTT TGCGTCCAGT 
AAACCAGTGT GCACGTCTCC AGTGGAGGAC ATTGATACAA CAGCACCACC TGGTGGCACA 
ATTGCGCAGC TGCCACCGCC AGCAAGCGCA GGGGAACAGC CGGATGCGTG CGTCTCAGCC 
GAGGGCTCTG GTTCTGGTCT GCAGGCTTCG CAACAGCAGC AGCAGAGGGT TTCCATCCAT 
AAACCGTTCA CACAGTCCCG AGTGCCGCCG GATCTGCCCA TGCATCCCGC TCCTCGACAC 
ATCACTGAGG AGGAGCTGCG GGTGCTGGAG AGCTGCCTGC ACCGCTGGAG GAGCGAGGTG 
GAGAACGACA CCAGAGATCT GCAGGCCAGC ATATCCAGAA TCCACAGAAC TATCGAGCTC 
ATGTATTCTG ACAAGTCTAT GATGCAGGTG CCGTATCGAC TACATGCTGT TCTGGTCCAC 
GAGGGTCAGG CGAATGCTGG TCACTACTGG GCCTACATTT ATGACCGACA TCACCAGCGC 
TGGATGAAAT ACAACGACAT TGCAGTGACA AAGTCATCAT GGGAAGAGCT AGTGCGGGAC 
TCGTTTGGTG GATATCGTAA CGCCAGCGCA TACTGCCTCA TGTACATTGA TGATAAGAAA 
CCCTTCCTTA TTCAAGAAGA GTTTGATAAA GAGACGGGAC AGATGCTCAG TGGTTTGGAC 
AAGCTTCCTC CTGACCTGAA GGTGTTTGTG GAGGCAGATA ACAAGCTCTT CGAGAGGGAG 
ATGGAGGAGT GGGATGCTTT ACAGGCCCGC AAACTCCAGC AGGAAAAGTT GGCTCTGGCT 
GCTGCTGCTG CGGCTGCTGC CGCCGCTAGT GTGGCCGTCC AGCCCATGAG CACTGAACCC 
TGCTCTCCAG ACAACACAGC TCCATCAGCA GCTCCTCAGC ATGACCCAGA GTACATGGAG 
CAACAGTCGC CCAGCGGAGA CTCCAAACAC CTGCAGGAGG ACACAGAGAG AGCCATCAGC 
AAGGCTGCAG CGGAGCATGA TGAGAAGAGT CCAGAAGCTC TGCTTAATAC ATCTCAGTCA 
TCTCACCCTG ACAGCAAGCT GACCTCTGAC CCCTGCCCTC CTCCTGACCC CGAGCAGGAC 
GACCCCACCT GCCCTCCACC CGGCCCTCAG CGGGTGGTGG AGGTGGCCAT TCCTAATGTA 
GGGACCTTCG TCATTCAGTC GAAGGAGGGA GGTTATGATG ACGAGGCGAT GATGACCCCA 
AACATGCAGG GTATCATAAT GGCCATTGGC AAATCCAGGA GTGTTTATGA CAAGTGTGGG 
CCTGAAGCAG CGTTCTTCAA GGCGATGAAG GTTGAGTACA CGCGTTTGCT GAGGCTTGCT 
CAGGAGGACA CGCCGCCCGA GAGAGACTAC CGGCTACAGC ATGTCATTGT GTTCTTTATT 
CACAATGAGG CGCCCAAGAA GATTGTGGAG AGAACATTGC TCATGCAGTT TGCTGACAGA 
AACCTTGGCT TCGATGAGAG GTGTAAAAGC ATCATGAAAG TGGCACGTGC CAAACTGGAG 
CTCATCAAGC CTGATGAGGT GAACTTGGAG GAGTACGAGA TGTGGCATCA GGACTACAGG 
ATTTTCCGTG AGACGTCAGT GTTCCTGTTG ATCGGCCTGG AATTATTCAT GAAGAAAAGT 
TTTGTGGAAG CGTTGATGTA TCTGATCTAC GCCTATCAGT ACAACAAAGA GCTTCTGGCG 
AAAGGGCCGT ACAGAGGCCA TGATGAGGAG CTGATCGCAC ATTACCGCAG AGAGTGCCTG 
CTGAAGTTAA ATGAATACGC TGCCGCTTTG TTCGAGACGG GCGAGGAGGC TAAAGTGAAC 
ACGGGTCTGA GCATCATGAA CGATCTGGTG GTGCCCTGTC TTCCTCTGTT ACTGGTGGAT 
GAGATGGAGG AGAAGGACAT GGTGGCTGTT GAAGACATGA GGAACCGATG GTGCTCATAC 
CTGGGCCAGG AAATGGAGCC GAATCTACAG GAGAAGCTGA CCGACTTCCT CCCCAAACTG 
CTGGACTGCT CGACCGAGAT CAAAAGCTTT CACGAGCCGC CCAAACTGCC CTCTTACTCC 
ACGCTGGAGC TGTTCGAGCG CTTCGGCAGG GTCATGACGT CCCTCACTAT GGGTCGAACC 
CCTGCAGATG GCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_009305559.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303834.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40923
Clone ID Related Accession (Same CDS sequence)	XM_009305559.2 , XM_009305559.3
Accession Version	XM_009305559.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA GTTTGATAAA GAGACGGGAC AGATGCTCAG TGGTTTGGAC 
AAGCTTCCTC CTGACCTGAA GGTGTTTGTG GAGGCAGATA ACAAGCTCTT CGAGAGGGAG 
ATGGAGGAGT GGGATGCTTT ACAGGCCCGC AAACTCCAGC AGGAAAAGTT GGCTCTGGCT 
GCTGCTGCTG CGGCTGCTGC CGCCGCTAGT GTGGCCGTCC AGCCCATGAG CACTGAACCC 
TGCTCTCCAG ACAACACAGC TCCATCAGCA GCTCCTCAGC ATGACCCAGA GTACATGGAG 
CAACAGTCGC CCAGCGGAGA CTCCAAACAC CTGCAGGAGG ACACAGAGAG AGCCATCAGC 
AAGGCTGCAG CGGAGCATGA TGAGAAGAGT CCAGAAGCTC TGCTTAATAC ATCTCAGTCA 
TCTCACCCTG ACAGCAAGCT GACCTCTGAC CCCTGCCCTC CTCCTGACCC CGAGCAGGAC 
GACCCCACCT GCCCTCCACC CGGCCCTCAG CGGGTGGTGG AGGTGGCCAT TCCTAATGTA 
GGGACCTTCG TCATTCAGTC GAAGGAGGGA GGTTATGATG ACGAGGCGAT GATGACCCCA 
AACATGCAGG GTATCATAAT GGCCATTGGC AAATCCAGGA GTGTTTATGA CAAGTGTGGG 
CCTGAAGCAG CGTTCTTCAA GGCGATGAAG GTTGAGTACA CGCGTTTGCT GAGGCTTGCT 
CAGGAGGACA CGCCGCCCGA GAGAGACTAC CGGCTACAGC ATGTCATTGT GTTCTTTATT 
CACAATGAGG CGCCCAAGAA GATTGTGGAG AGAACATTGC TCATGCAGTT TGCTGACAGA 
AACCTTGGCT TCGATGAGAG GTGTAAAAGC ATCATGAAAG TGGCACGTGC CAAACTGGAG 
CTCATCAAGC CTGATGAGGT GAACTTGGAG GAGTACGAGA TGTGGCATCA GGACTACAGG 
ATTTTCCGTG AGACGTCAGT GTTCCTGTTG ATCGGCCTGG AATTATTCAT GAAGAAAAGT 
TTTGTGGAAG CGTTGATGTA TCTGATCTAC GCCTATCAGT ACAACAAAGA GCTTCTGGCG 
AAAGGGCCGT ACAGAGGCCA TGATGAGGAG CTGATCGCAC ATTACCGCAG AGAGTGCCTG 
CTGAAGTTAA ATGAATACGC TGCCGCTTTG TTCGAGACGG GCGAGGAGGC TAAAGTGAAC 
ACGGGTCTGA GCATCATGAA CGATCTGGTG GTGCCCTGTC TTCCTCTGTT ACTGGTGGAT 
GAGATGGAGG AGAAGGACAT GGTGGCTGTT GAAGACATGA GGAACCGATG GTGCTCATAC 
CTGGGCCAGG AAATGGAGCC GAATCTACAG GAGAAGCTGA CCGACTTCCT CCCCAAACTG 
CTGGACTGCT CGACCGAGAT CAAAAGCTTT CACGAGCCGC CCAAACTGCC CTCTTACTCC 
ACGCTGGAGC TGTTCGAGCG CTTCGGCAGG GTCATGACGT CCCTCACTAT GGGTCGAACC 
CCTGCAGATG GCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303834.1
CDS	207..3704
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPSAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTSQSSHPDSKLTSDPCPPPDPEQDDPTCPPPGPQRVVEVAIPNVGTFVIQSKEGGYDDEAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_009305559.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305559.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA GTTTGATAAA GAGACGGGAC AGATGCTCAG TGGTTTGGAC 
AAGCTTCCTC CTGACCTGAA GGTGTTTGTG GAGGCAGATA ACAAGCTCTT CGAGAGGGAG 
ATGGAGGAGT GGGATGCTTT ACAGGCCCGC AAACTCCAGC AGGAAAAGTT GGCTCTGGCT 
GCTGCTGCTG CGGCTGCTGC CGCCGCTAGT GTGGCCGTCC AGCCCATGAG CACTGAACCC 
TGCTCTCCAG ACAACACAGC TCCATCAGCA GCTCCTCAGC ATGACCCAGA GTACATGGAG 
CAACAGTCGC CCAGCGGAGA CTCCAAACAC CTGCAGGAGG ACACAGAGAG AGCCATCAGC 
AAGGCTGCAG CGGAGCATGA TGAGAAGAGT CCAGAAGCTC TGCTTAATAC ATCTCAGTCA 
TCTCACCCTG ACAGCAAGCT GACCTCTGAC CCCTGCCCTC CTCCTGACCC CGAGCAGGAC 
GACCCCACCT GCCCTCCACC CGGCCCTCAG CGGGTGGTGG AGGTGGCCAT TCCTAATGTA 
GGGACCTTCG TCATTCAGTC GAAGGAGGGA GGTTATGATG ACGAGGCGAT GATGACCCCA 
AACATGCAGG GTATCATAAT GGCCATTGGC AAATCCAGGA GTGTTTATGA CAAGTGTGGG 
CCTGAAGCAG CGTTCTTCAA GGCGATGAAG GTTGAGTACA CGCGTTTGCT GAGGCTTGCT 
CAGGAGGACA CGCCGCCCGA GAGAGACTAC CGGCTACAGC ATGTCATTGT GTTCTTTATT 
CACAATGAGG CGCCCAAGAA GATTGTGGAG AGAACATTGC TCATGCAGTT TGCTGACAGA 
AACCTTGGCT TCGATGAGAG GTGTAAAAGC ATCATGAAAG TGGCACGTGC CAAACTGGAG 
CTCATCAAGC CTGATGAGGT GAACTTGGAG GAGTACGAGA TGTGGCATCA GGACTACAGG 
ATTTTCCGTG AGACGTCAGT GTTCCTGTTG ATCGGCCTGG AATTATTCAT GAAGAAAAGT 
TTTGTGGAAG CGTTGATGTA TCTGATCTAC GCCTATCAGT ACAACAAAGA GCTTCTGGCG 
AAAGGGCCGT ACAGAGGCCA TGATGAGGAG CTGATCGCAC ATTACCGCAG AGAGTGCCTG 
CTGAAGTTAA ATGAATACGC TGCCGCTTTG TTCGAGACGG GCGAGGAGGC TAAAGTGAAC 
ACGGGTCTGA GCATCATGAA CGATCTGGTG GTGCCCTGTC TTCCTCTGTT ACTGGTGGAT 
GAGATGGAGG AGAAGGACAT GGTGGCTGTT GAAGACATGA GGAACCGATG GTGCTCATAC 
CTGGGCCAGG AAATGGAGCC GAATCTACAG GAGAAGCTGA CCGACTTCCT CCCCAAACTG 
CTGGACTGCT CGACCGAGAT CAAAAGCTTT CACGAGCCGC CCAAACTGCC CTCTTACTCC 
ACGCTGGAGC TGTTCGAGCG CTTCGGCAGG GTCATGACGT CCCTCACTAT GGGTCGAACC 
CCTGCAGATG GCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_009305559.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303834.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa40923
Clone ID Related Accession (Same CDS sequence)	XM_009305559.2 , XM_009305559.3
Accession Version	XM_009305559.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305559.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA GTTTGATAAA GAGACGGGAC AGATGCTCAG TGGTTTGGAC 
AAGCTTCCTC CTGACCTGAA GGTGTTTGTG GAGGCAGATA ACAAGCTCTT CGAGAGGGAG 
ATGGAGGAGT GGGATGCTTT ACAGGCCCGC AAACTCCAGC AGGAAAAGTT GGCTCTGGCT 
GCTGCTGCTG CGGCTGCTGC CGCCGCTAGT GTGGCCGTCC AGCCCATGAG CACTGAACCC 
TGCTCTCCAG ACAACACAGC TCCATCAGCA GCTCCTCAGC ATGACCCAGA GTACATGGAG 
CAACAGTCGC CCAGCGGAGA CTCCAAACAC CTGCAGGAGG ACACAGAGAG AGCCATCAGC 
AAGGCTGCAG CGGAGCATGA TGAGAAGAGT CCAGAAGCTC TGCTTAATAC ATCTCAGTCA 
TCTCACCCTG ACAGCAAGCT GACCTCTGAC CCCTGCCCTC CTCCTGACCC CGAGCAGGAC 
GACCCCACCT GCCCTCCACC CGGCCCTCAG CGGGTGGTGG AGGTGGCCAT TCCTAATGTA 
GGGACCTTCG TCATTCAGTC GAAGGAGGGA GGTTATGATG ACGAGGCGAT GATGACCCCA 
AACATGCAGG GTATCATAAT GGCCATTGGC AAATCCAGGA GTGTTTATGA CAAGTGTGGG 
CCTGAAGCAG CGTTCTTCAA GGCGATGAAG GTTGAGTACA CGCGTTTGCT GAGGCTTGCT 
CAGGAGGACA CGCCGCCCGA GAGAGACTAC CGGCTACAGC ATGTCATTGT GTTCTTTATT 
CACAATGAGG CGCCCAAGAA GATTGTGGAG AGAACATTGC TCATGCAGTT TGCTGACAGA 
AACCTTGGCT TCGATGAGAG GTGTAAAAGC ATCATGAAAG TGGCACGTGC CAAACTGGAG 
CTCATCAAGC CTGATGAGGT GAACTTGGAG GAGTACGAGA TGTGGCATCA GGACTACAGG 
ATTTTCCGTG AGACGTCAGT GTTCCTGTTG ATCGGCCTGG AATTATTCAT GAAGAAAAGT 
TTTGTGGAAG CGTTGATGTA TCTGATCTAC GCCTATCAGT ACAACAAAGA GCTTCTGGCG 
AAAGGGCCGT ACAGAGGCCA TGATGAGGAG CTGATCGCAC ATTACCGCAG AGAGTGCCTG 
CTGAAGTTAA ATGAATACGC TGCCGCTTTG TTCGAGACGG GCGAGGAGGC TAAAGTGAAC 
ACGGGTCTGA GCATCATGAA CGATCTGGTG GTGCCCTGTC TTCCTCTGTT ACTGGTGGAT 
GAGATGGAGG AGAAGGACAT GGTGGCTGTT GAAGACATGA GGAACCGATG GTGCTCATAC 
CTGGGCCAGG AAATGGAGCC GAATCTACAG GAGAAGCTGA CCGACTTCCT CCCCAAACTG 
CTGGACTGCT CGACCGAGAT CAAAAGCTTT CACGAGCCGC CCAAACTGCC CTCTTACTCC 
ACGCTGGAGC TGTTCGAGCG CTTCGGCAGG GTCATGACGT CCCTCACTAT GGGTCGAACC 
CCTGCAGATG GCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303834.1
CDS	210..3707
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPSAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTSQSSHPDSKLTSDPCPPPDPEQDDPTCPPPGPQRVVEVAIPNVGTFVIQSKEGGYDDEAMMTPNMQGIIMAIGKSRSVYDKCGPEAAFFKAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_009305559.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305559.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA GTTTGATAAA GAGACGGGAC AGATGCTCAG TGGTTTGGAC 
AAGCTTCCTC CTGACCTGAA GGTGTTTGTG GAGGCAGATA ACAAGCTCTT CGAGAGGGAG 
ATGGAGGAGT GGGATGCTTT ACAGGCCCGC AAACTCCAGC AGGAAAAGTT GGCTCTGGCT 
GCTGCTGCTG CGGCTGCTGC CGCCGCTAGT GTGGCCGTCC AGCCCATGAG CACTGAACCC 
TGCTCTCCAG ACAACACAGC TCCATCAGCA GCTCCTCAGC ATGACCCAGA GTACATGGAG 
CAACAGTCGC CCAGCGGAGA CTCCAAACAC CTGCAGGAGG ACACAGAGAG AGCCATCAGC 
AAGGCTGCAG CGGAGCATGA TGAGAAGAGT CCAGAAGCTC TGCTTAATAC ATCTCAGTCA 
TCTCACCCTG ACAGCAAGCT GACCTCTGAC CCCTGCCCTC CTCCTGACCC CGAGCAGGAC 
GACCCCACCT GCCCTCCACC CGGCCCTCAG CGGGTGGTGG AGGTGGCCAT TCCTAATGTA 
GGGACCTTCG TCATTCAGTC GAAGGAGGGA GGTTATGATG ACGAGGCGAT GATGACCCCA 
AACATGCAGG GTATCATAAT GGCCATTGGC AAATCCAGGA GTGTTTATGA CAAGTGTGGG 
CCTGAAGCAG CGTTCTTCAA GGCGATGAAG GTTGAGTACA CGCGTTTGCT GAGGCTTGCT 
CAGGAGGACA CGCCGCCCGA GAGAGACTAC CGGCTACAGC ATGTCATTGT GTTCTTTATT 
CACAATGAGG CGCCCAAGAA GATTGTGGAG AGAACATTGC TCATGCAGTT TGCTGACAGA 
AACCTTGGCT TCGATGAGAG GTGTAAAAGC ATCATGAAAG TGGCACGTGC CAAACTGGAG 
CTCATCAAGC CTGATGAGGT GAACTTGGAG GAGTACGAGA TGTGGCATCA GGACTACAGG 
ATTTTCCGTG AGACGTCAGT GTTCCTGTTG ATCGGCCTGG AATTATTCAT GAAGAAAAGT 
TTTGTGGAAG CGTTGATGTA TCTGATCTAC GCCTATCAGT ACAACAAAGA GCTTCTGGCG 
AAAGGGCCGT ACAGAGGCCA TGATGAGGAG CTGATCGCAC ATTACCGCAG AGAGTGCCTG 
CTGAAGTTAA ATGAATACGC TGCCGCTTTG TTCGAGACGG GCGAGGAGGC TAAAGTGAAC 
ACGGGTCTGA GCATCATGAA CGATCTGGTG GTGCCCTGTC TTCCTCTGTT ACTGGTGGAT 
GAGATGGAGG AGAAGGACAT GGTGGCTGTT GAAGACATGA GGAACCGATG GTGCTCATAC 
CTGGGCCAGG AAATGGAGCC GAATCTACAG GAGAAGCTGA CCGACTTCCT CCCCAAACTG 
CTGGACTGCT CGACCGAGAT CAAAAGCTTT CACGAGCCGC CCAAACTGCC CTCTTACTCC 
ACGCTGGAGC TGTTCGAGCG CTTCGGCAGG GTCATGACGT CCCTCACTAT GGGTCGAACC 
CCTGCAGATG GCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_009305560.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303835.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa40924
Clone ID Related Accession (Same CDS sequence)	XM_009305560.3 , XM_009305560.2
Accession Version	XM_009305560.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCATCA GCAGCTCCTC AGCATGACCC AGAGTACATG 
GAGCAACAGT CGCCCAGCGG AGACTCCAAA CACCTGCAGG AGGACACAGA GAGAGCCATC 
AGCAAGGCTG CAGCGGAGCA TGATGAGAAG AGTCCAGAAG CTCTGCTTAA TACAGCGATG 
AAGGTTGAGT ACACGCGTTT GCTGAGGCTT GCTCAGGAGG ACACGCCGCC CGAGAGAGAC 
TACCGGCTAC AGCATGTCAT TGTGTTCTTT ATTCACAATG AGGCGCCCAA GAAGATTGTG 
GAGAGAACAT TGCTCATGCA GTTTGCTGAC AGAAACCTTG GCTTCGATGA GAGGTGTAAA 
AGCATCATGA AAGTGGCACG TGCCAAACTG GAGCTCATCA AGCCTGATGA GGTGAACTTG 
GAGGAGTACG AGATGTGGCA TCAGGACTAC AGGATTTTCC GTGAGACGTC AGTGTTCCTG 
TTGATCGGCC TGGAATTATT CATGAAGAAA AGTTTTGTGG AAGCGTTGAT GTATCTGATC 
TACGCCTATC AGTACAACAA AGAGCTTCTG GCGAAAGGGC CGTACAGAGG CCATGATGAG 
GAGCTGATCG CACATTACCG CAGAGAGTGC CTGCTGAAGT TAAATGAATA CGCTGCCGCT 
TTGTTCGAGA CGGGCGAGGA GGCTAAAGTG AACACGGGTC TGAGCATCAT GAACGATCTG 
GTGGTGCCCT GTCTTCCTCT GTTACTGGTG GATGAGATGG AGGAGAAGGA CATGGTGGCT 
GTTGAAGACA TGAGGAACCG ATGGTGCTCA TACCTGGGCC AGGAAATGGA GCCGAATCTA 
CAGGAGAAGC TGACCGACTT CCTCCCCAAA CTGCTGGACT GCTCGACCGA GATCAAAAGC 
TTTCACGAGC CGCCCAAACT GCCCTCTTAC TCCACGCTGG AGCTGTTCGA GCGCTTCGGC 
AGGGTCATGA CGTCCCTCAC TATGGGTCGA ACCCCTGCAG ATGGCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303835.1
CDS	208..3438
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPSAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_009305560.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305560.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCATCA GCAGCTCCTC AGCATGACCC AGAGTACATG 
GAGCAACAGT CGCCCAGCGG AGACTCCAAA CACCTGCAGG AGGACACAGA GAGAGCCATC 
AGCAAGGCTG CAGCGGAGCA TGATGAGAAG AGTCCAGAAG CTCTGCTTAA TACAGCGATG 
AAGGTTGAGT ACACGCGTTT GCTGAGGCTT GCTCAGGAGG ACACGCCGCC CGAGAGAGAC 
TACCGGCTAC AGCATGTCAT TGTGTTCTTT ATTCACAATG AGGCGCCCAA GAAGATTGTG 
GAGAGAACAT TGCTCATGCA GTTTGCTGAC AGAAACCTTG GCTTCGATGA GAGGTGTAAA 
AGCATCATGA AAGTGGCACG TGCCAAACTG GAGCTCATCA AGCCTGATGA GGTGAACTTG 
GAGGAGTACG AGATGTGGCA TCAGGACTAC AGGATTTTCC GTGAGACGTC AGTGTTCCTG 
TTGATCGGCC TGGAATTATT CATGAAGAAA AGTTTTGTGG AAGCGTTGAT GTATCTGATC 
TACGCCTATC AGTACAACAA AGAGCTTCTG GCGAAAGGGC CGTACAGAGG CCATGATGAG 
GAGCTGATCG CACATTACCG CAGAGAGTGC CTGCTGAAGT TAAATGAATA CGCTGCCGCT 
TTGTTCGAGA CGGGCGAGGA GGCTAAAGTG AACACGGGTC TGAGCATCAT GAACGATCTG 
GTGGTGCCCT GTCTTCCTCT GTTACTGGTG GATGAGATGG AGGAGAAGGA CATGGTGGCT 
GTTGAAGACA TGAGGAACCG ATGGTGCTCA TACCTGGGCC AGGAAATGGA GCCGAATCTA 
CAGGAGAAGC TGACCGACTT CCTCCCCAAA CTGCTGGACT GCTCGACCGA GATCAAAAGC 
TTTCACGAGC CGCCCAAACT GCCCTCTTAC TCCACGCTGG AGCTGTTCGA GCGCTTCGGC 
AGGGTCATGA CGTCCCTCAC TATGGGTCGA ACCCCTGCAG ATGGCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_009305560.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303835.1 Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa40924
Clone ID Related Accession (Same CDS sequence)	XM_009305560.3 , XM_009305560.2
Accession Version	XM_009305560.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCATCA GCAGCTCCTC AGCATGACCC AGAGTACATG 
GAGCAACAGT CGCCCAGCGG AGACTCCAAA CACCTGCAGG AGGACACAGA GAGAGCCATC 
AGCAAGGCTG CAGCGGAGCA TGATGAGAAG AGTCCAGAAG CTCTGCTTAA TACAGCGATG 
AAGGTTGAGT ACACGCGTTT GCTGAGGCTT GCTCAGGAGG ACACGCCGCC CGAGAGAGAC 
TACCGGCTAC AGCATGTCAT TGTGTTCTTT ATTCACAATG AGGCGCCCAA GAAGATTGTG 
GAGAGAACAT TGCTCATGCA GTTTGCTGAC AGAAACCTTG GCTTCGATGA GAGGTGTAAA 
AGCATCATGA AAGTGGCACG TGCCAAACTG GAGCTCATCA AGCCTGATGA GGTGAACTTG 
GAGGAGTACG AGATGTGGCA TCAGGACTAC AGGATTTTCC GTGAGACGTC AGTGTTCCTG 
TTGATCGGCC TGGAATTATT CATGAAGAAA AGTTTTGTGG AAGCGTTGAT GTATCTGATC 
TACGCCTATC AGTACAACAA AGAGCTTCTG GCGAAAGGGC CGTACAGAGG CCATGATGAG 
GAGCTGATCG CACATTACCG CAGAGAGTGC CTGCTGAAGT TAAATGAATA CGCTGCCGCT 
TTGTTCGAGA CGGGCGAGGA GGCTAAAGTG AACACGGGTC TGAGCATCAT GAACGATCTG 
GTGGTGCCCT GTCTTCCTCT GTTACTGGTG GATGAGATGG AGGAGAAGGA CATGGTGGCT 
GTTGAAGACA TGAGGAACCG ATGGTGCTCA TACCTGGGCC AGGAAATGGA GCCGAATCTA 
CAGGAGAAGC TGACCGACTT CCTCCCCAAA CTGCTGGACT GCTCGACCGA GATCAAAAGC 
TTTCACGAGC CGCCCAAACT GCCCTCTTAC TCCACGCTGG AGCTGTTCGA GCGCTTCGGC 
AGGGTCATGA CGTCCCTCAC TATGGGTCGA ACCCCTGCAG ATGGCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303835.1
CDS	207..3437
Translation	MTVEQNVLQQHSQKHQQTLLNQLREVTGTTDVQLLQQALQVSNGDLVEAVAFLTEKNAKVPQQDEATYYQTAQITNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHERKRMENCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLHYSPPARMQDLPRNQKEHRNLPFMQELRHLFSLLVGSKRKYVDPSGAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQIKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTVLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTTAPPGGTIAQLPPPASAGEQPDACVSAEGSGSGLQASQQQQQRVSIHKPFTQSRVPPDLPMHPAPRHITEEELRVLESCLHRWRSEVENDTRDLQASISRIHRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDRHHQRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYIDDKKPFLIQEEFDKETGQMLSGLDKLPPDLKVFVEADNKLFEREMEEWDALQARKLQQEKLALAAAAAAAAAASVAVQPMSTEPCSPDNTAPSAAPQHDPEYMEQQSPSGDSKHLQEDTERAISKAAAEHDEKSPEALLNTAMKVEYTRLLRLAQEDTPPERDYRLQHVIVFFIHNEAPKKIVERTLLMQFADRNLGFDERCKSIMKVARAKLELIKPDEVNLEEYEMWHQDYRIFRETSVFLLIGLELFMKKSFVEALMYLIYAYQYNKELLAKGPYRGHDEELIAHYRRECLLKLNEYAAALFETGEEAKVNTGLSIMNDLVVPCLPLLLVDEMEEKDMVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKSFHEPPKLPSYSTLELFERFGRVMTSLTMGRTPADGR

Target ORF information:

RefSeq Version	XM_009305560.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 25 (usp25), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305560.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTGCAGCAG CACTCTCAGA AGCACCAGCA GACATTACTG 
AACCAGCTGA GGGAAGTTAC TGGAACTACA GATGTTCAAC TGTTACAGCA GGCACTGCAG 
GTCAGTAATG GTGACCTAGT GGAGGCTGTT GCCTTTCTCA CTGAGAAGAA CGCCAAGGTC 
CCGCAGCAAG ATGAAGCCAC CTATTACCAG ACGGCCCAAA TCACCAACGA CAGATACATC 
AGCGTGGGCA GCCAGGCTGA CACAAATGTA ATTGACCTGA CTGGAGATGA TAAAGATGAC 
CTCCAGCGGG CCATCGCTCT CAGTCTGGAG GAGTCAAACA GAGCCTTCAG GGAGACGGGC 
ATCACCGATG AAGAGCAGGC CATCAGCAGA GTCCTGGAGG CGAGCATCGC AGAGAACAAG 
GCCAGCCTGA AGCGAACACA CACTGAAGTG TGGAGCGATT CACCAAACCC TCACGAGCGC 
AAGAGAATGG AGAACTGTCC TGTAGGACTG AAGAATGTGG GAAACACTTG CTGGTTCAGT 
GCTGTTATAC AGTCTCTTTT TAACCTGCTG GAGTTTCAGA GGCTGGTGTT GCATTATTCC 
CCTCCTGCAC GGATGCAGGA CCTGCCCCGC AATCAAAAGG AGCACAGGAA CCTGCCCTTC 
ATGCAGGAGC TTAGGCATCT CTTCTCCTTG TTGGTGGGCT CCAAGCGGAA GTATGTGGAC 
CCGTCCGGGG CCGTGGAGAT CCTCAAAGAT GCCTTCAAGT CCAGTGAGTC TCAGCAGCAG 
GATGTGAGCG AGTTCACCCA TAAACTTCTG GACTGGCTGG AGGACGCATT TCAGATTAAA 
GCAGAAGAGG ACAGGGAGGG AGAGAAACCC AAGAACCCGA TGGTTGAGCT GTTTTATGGA 
CGTTTCCTGG CTGTGGGCGT CCTGGAGGGA AAGAAATTTG AAAACACTGA GATGTTTGGC 
CAGTATCCTC TCCAGGTGAA CGGCTTCAAA GATCTACACG AGTGTTTAGA GGCCGCCATG 
ATAGAGGGAG AAATCGAGTC TTTGCATTCT GCAGAAAACT CCGCCAAATC AGGCCAAGAG 
CATTGGTTCA CAGAGCTTCC ACCCGTTTTG ACCTTTGAAC TGTCCAGGTT TGAGTTTAAT 
CAAGCCTTAG GCCGACCTGA GAAGATTCAC AATAAGCTCG AGTTTCCTTC TATGCTGTAC 
ATGGACAGGT ACATGGACAG GAACCGAGAG ATCACACGGA TAAAGCGAGA GGAGATCAGA 
AGACTAAAGG AGCACCTCAC CGTCCTCCAG CAGCGACTCG AGAGATATCT GAGTTATGGC 
TCGGGGCCCA AGCGCTTTCC TCTGGCTGAT GTTCTCCAAT ATGCCATGGA GTTTGCGTCC 
AGTAAACCAG TGTGCACGTC TCCAGTGGAG GACATTGATA CAACAGCACC ACCTGGTGGC 
ACAATTGCGC AGCTGCCACC GCCAGCAAGC GCAGGGGAAC AGCCGGATGC GTGCGTCTCA 
GCCGAGGGCT CTGGTTCTGG TCTGCAGGCT TCGCAACAGC AGCAGCAGAG GGTTTCCATC 
CATAAACCGT TCACACAGTC CCGAGTGCCG CCGGATCTGC CCATGCATCC CGCTCCTCGA 
CACATCACTG AGGAGGAGCT GCGGGTGCTG GAGAGCTGCC TGCACCGCTG GAGGAGCGAG 
GTGGAGAACG ACACCAGAGA TCTGCAGGCC AGCATATCCA GAATCCACAG AACTATCGAG 
CTCATGTATT CTGACAAGTC TATGATGCAG GTGCCGTATC GACTACATGC TGTTCTGGTC 
CACGAGGGTC AGGCGAATGC TGGTCACTAC TGGGCCTACA TTTATGACCG ACATCACCAG 
CGCTGGATGA AATACAACGA CATTGCAGTG ACAAAGTCAT CATGGGAAGA GCTAGTGCGG 
GACTCGTTTG GTGGATATCG TAACGCCAGC GCATACTGCC TCATGTACAT TGATGATAAG 
AAACCCTTCC TTATTCAAGA AGAGTTTGAT AAAGAGACGG GACAGATGCT CAGTGGTTTG 
GACAAGCTTC CTCCTGACCT GAAGGTGTTT GTGGAGGCAG ATAACAAGCT CTTCGAGAGG 
GAGATGGAGG AGTGGGATGC TTTACAGGCC CGCAAACTCC AGCAGGAAAA GTTGGCTCTG 
GCTGCTGCTG CTGCGGCTGC TGCCGCCGCT AGTGTGGCCG TCCAGCCCAT GAGCACTGAA 
CCCTGCTCTC CAGACAACAC AGCTCCATCA GCAGCTCCTC AGCATGACCC AGAGTACATG 
GAGCAACAGT CGCCCAGCGG AGACTCCAAA CACCTGCAGG AGGACACAGA GAGAGCCATC 
AGCAAGGCTG CAGCGGAGCA TGATGAGAAG AGTCCAGAAG CTCTGCTTAA TACAGCGATG 
AAGGTTGAGT ACACGCGTTT GCTGAGGCTT GCTCAGGAGG ACACGCCGCC CGAGAGAGAC 
TACCGGCTAC AGCATGTCAT TGTGTTCTTT ATTCACAATG AGGCGCCCAA GAAGATTGTG 
GAGAGAACAT TGCTCATGCA GTTTGCTGAC AGAAACCTTG GCTTCGATGA GAGGTGTAAA 
AGCATCATGA AAGTGGCACG TGCCAAACTG GAGCTCATCA AGCCTGATGA GGTGAACTTG 
GAGGAGTACG AGATGTGGCA TCAGGACTAC AGGATTTTCC GTGAGACGTC AGTGTTCCTG 
TTGATCGGCC TGGAATTATT CATGAAGAAA AGTTTTGTGG AAGCGTTGAT GTATCTGATC 
TACGCCTATC AGTACAACAA AGAGCTTCTG GCGAAAGGGC CGTACAGAGG CCATGATGAG 
GAGCTGATCG CACATTACCG CAGAGAGTGC CTGCTGAAGT TAAATGAATA CGCTGCCGCT 
TTGTTCGAGA CGGGCGAGGA GGCTAAAGTG AACACGGGTC TGAGCATCAT GAACGATCTG 
GTGGTGCCCT GTCTTCCTCT GTTACTGGTG GATGAGATGG AGGAGAAGGA CATGGTGGCT 
GTTGAAGACA TGAGGAACCG ATGGTGCTCA TACCTGGGCC AGGAAATGGA GCCGAATCTA 
CAGGAGAAGC TGACCGACTT CCTCCCCAAA CTGCTGGACT GCTCGACCGA GATCAAAAGC 
TTTCACGAGC CGCCCAAACT GCCCTCTTAC TCCACGCTGG AGCTGTTCGA GCGCTTCGGC 
AGGGTCATGA CGTCCCTCAC TATGGGTCGA ACCCCTGCAG ATGGCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	usp25
Entrez Gene ID	406790
Full Name	ubiquitin specific peptidase 25
Synonyms	wu:fi33c05,wu:fo67e06,wu:fp97a04,zgc:55791
General protein information	Preferred Names ubiquitin specific peptidase 25 Names ubiquitin carboxyl-terminal hydrolase 25 ubiquitin specific protease 25
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Mus musculus (house mouse)	Usp25	NP_038946.2
Rattus norvegicus (Norway rat)	Usp25	NP_001100584.1
Homo sapiens (human)	USP25	NP_001269970.1
	USP25	XP_001107973.2
Canis lupus familiaris (dog)	USP25	XP_535562.3
Xenopus tropicalis (tropical clawed frog)	usp25	NP_001039152.1
Bos taurus (cattle)	USP25	XP_005201215.1
Gallus gallus (chicken)	USP25	XP_004938410.1
Pan troglodytes (chimpanzee)	USP25	XP_531393.2
Danio rerio (zebrafish)	usp25	NP_001001886.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

usp25 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム