rbm22 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rbm22
Entrez Gene ID	406495
Full Name	RNA binding motif protein 22
Synonyms	fb37a01,wu:fb37a01,wu:fc62e03,wu:fe05c04,zgc:77910
General protein information	Preferred Names RNA binding motif protein 22 Names pre-mRNA-splicing factor RBM22 fc62e03 fe05c04
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213214.1	NP_998379.1	pre-mRNA-splicing factor RBM22

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RBM22	XP_001167240.1
	RBM22	XP_001108861.1
Bos taurus (cattle)	RBM22	NP_001032907.1
Caenorhabditis elegans (roundworm)	T11G6.8	NP_502014.2
Arabidopsis thaliana (thale cress)	AT2G29580	NP_180518.1
Mus musculus (house mouse)	Rbm22	NP_080052.1
Rattus norvegicus (Norway rat)	Rbm22	NP_001020847.1
Danio rerio (zebrafish)	LOC100536002	XP_003200931.1
Gallus gallus (chicken)	RBM22	NP_001006151.1
Danio rerio (zebrafish)	LOC100536641	XP_003200934.1
Drosophila melanogaster (fruit fly)	CG14641	NP_649440.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004509	XP_003436831.1
Homo sapiens (human)	RBM22	NP_060517.1
Danio rerio (zebrafish)	rbm22	NP_998379.1
Arabidopsis thaliana (thale cress)	AT1G07360	NP_563788.1
Oryza sativa (rice)	Os06g0170500	NP_001056931.1
Canis lupus familiaris (dog)	RBM22	XP_546302.2
Oryza sativa (rice)	Os07g0281000	NP_001059374.1
Xenopus tropicalis (tropical clawed frog)	rbm22	NP_989108.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evolutionary plasticity of segmentation clock networks.
Krol AJ, Roellig D, Dequ?ant ML, Tassy O, Glynn E, Hattem G, Mushegian A, Oates AC, Pourqui? O
Development (Cambridge, England)138(13)2783-92(2011 Jul)

Spatiotemporal compartmentalization of key physiological processes during muscle precursor differentiation.
Ozbudak EM, Tassy O, Pourqui? O
Proceedings of the National Academy of Sciences of the United States of America107(9)4224-9(2010 Mar)

RNA-binding motif protein RBM22 is required for normal development of zebrafish embryos.
He F, Wang CT, Gou LT
Genetics and molecular research : GMR8(4)1466-73(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RT-PCR analysis confirmed the in situ expression pattern, indicating that RBM22 is expressed without any spatial and temporal specificity during zebrafish development
Title: RNA-binding motif protein RBM22 is required for normal development of zebrafish embryos.

The following rbm22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rbm22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37705	NM_213214.1 Latest version!	Danio rerio RNA binding motif protein 22 (rbm22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rbm22 ( NM_213214.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998379.1 Danio rerio RNA binding motif protein 22 (rbm22), mRNA.

Price & Availability↑

CloneID	ODa37705
Clone ID Related Accession (Same CDS sequence)	NM_213214.1
Accession Version	NM_213214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	pre-mRNA-splicing factor RBM22
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC065892.1. ##Evidence-Data-START## Transcript exon combination :: BC065892.1, BC153463.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGACGT CTTTGGGATC CAACACTTAT AACAGGCAAA ACTGGGAAGA TTCGGATTTT 
CCCATCCTCT GTCAAACATG CTTGGGAGAA AACCCTTACA TTCGCATGAC GAAAGAGAAA 
TTTGGCAAGG AGTGCAAGAT CTGTGCACGT CCCTTCACAG TTTTCCGCTG GTGTCCTGGA 
GTCAGGATGC GCTTTAAGAA GACAGAAGTG TGTCAGACCT GTAGTAAAAT GAAGAACGTT 
TGTCAGACCT GCCTCCTAGA TCTGGAATAT GGTTTGCCCA TTCAGGTTCG TGACACTGGG 
CTCTCCGTTA AGGATGAAGT ACCGCGATCT GATGTGAATA AGGAGTATTA CACTCAGAAT 
ATGGAAAGAG AGATTGCCAA CTCTGATGGC ACGAGACCTG TGGGTTTGCT TGGAAAGGCT 
CCGAGCAGCA GTGATATGCT GCTGAAACTG GCGCGCACCA CACCGTACTA CAAGAGGAAC 
AGACCACACA TCTGTTCCTT CTGGGTTAAA GGAGAGTGCA AAAGAGGAGA AGAGTGTCCG 
TACAGGCATG AAAAACCCAC TGATCCAGAT GACCCCCTGG CTGATCAGAA CATTAAGGAC 
CGATACTATG GAATTAATGA TCCTGTGGCT AATAAACTGC TGATGCGAGC CTCCACCATG 
CCCAGACTGG ACGTACCGGA CGACAAGTCC ATAACCACAC TTTATATTGG AGGCCTGGGA 
GAAAACGTCA CAGACTCGGA GCTCCGGAAT CACTTTTACC AGTTTGGAGA GATTCGGACC 
ATCACTATTG TCCAGAGGCA GCAGTGTGCT TTTATTCAGT TTGCCACTCG ACAGGCTGCA 
GAAACAGCTG CGGAGAAGTC CTTCAATAAA CTCATCATCA ATGGACGTCG TCTTAATGTT 
AAATGGGGCA GGTCCCAGGC GGCACGAGGG AAAGGAGAGA AGGACGGGGT GACGGAGAGT 
GGCATCAGAC TGGAGCCGGT GCCAGGGCTT CCTGGTGCTC TGCCTCCACC ACCTGTCTCT 
GATGAAGATG CGTCCACCAA TTACTTCAAT CTGGCTCCCA CACCCTCCCC TGCTGTCATG 
AACCTGGGTC TTCCTCCTCC ACCCGGGGTG ACCATGCCTC CTCCACCTGG ATTTGGTCCT 
CCTATGTTCC ACACTATGGA CCCCATGGCC CCGCCGGTGC CACCTCCAAT GGCCCTGAGA 
CCACCCGGAC AGGTCCACTA CCCATCTCAG GATCCTCAGA GGATGGGTGC CCACGCCAGT 
CGCCATGGAG GCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998379.1
CDS	14..1291
Translation	MATSLGSNTYNRQNWEDSDFPILCQTCLGENPYIRMTKEKFGKECKICARPFTVFRWCPGVRMRFKKTEVCQTCSKMKNVCQTCLLDLEYGLPIQVRDTGLSVKDEVPRSDVNKEYYTQNMEREIANSDGTRPVGLLGKAPSSSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECPYRHEKPTDPDDPLADQNIKDRYYGINDPVANKLLMRASTMPRLDVPDDKSITTLYIGGLGENVTDSELRNHFYQFGEIRTITIVQRQQCAFIQFATRQAAETAAEKSFNKLIINGRRLNVKWGRSQAARGKGEKDGVTESGIRLEPVPGLPGALPPPPVSDEDASTNYFNLAPTPSPAVMNLGLPPPPGVTMPPPPGFGPPMFHTMDPMAPPVPPPMALRPPGQVHYPSQDPQRMGAHASRHGGP

Target ORF information:

RefSeq Version	NM_213214.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RNA binding motif protein 22 (rbm22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213214.1

ORF Insert Sequence:

ATGGCGACGT CTTTGGGATC CAACACTTAT AACAGGCAAA ACTGGGAAGA TTCGGATTTT 
CCCATCCTCT GTCAAACATG CTTGGGAGAA AACCCTTACA TTCGCATGAC GAAAGAGAAA 
TTTGGCAAGG AGTGCAAGAT CTGTGCACGT CCCTTCACAG TTTTCCGCTG GTGTCCTGGA 
GTCAGGATGC GCTTTAAGAA GACAGAAGTG TGTCAGACCT GTAGTAAAAT GAAGAACGTT 
TGTCAGACCT GCCTCCTAGA TCTGGAATAT GGTTTGCCCA TTCAGGTTCG TGACACTGGG 
CTCTCCGTTA AGGATGAAGT ACCGCGATCT GATGTGAATA AGGAGTATTA CACTCAGAAT 
ATGGAAAGAG AGATTGCCAA CTCTGATGGC ACGAGACCTG TGGGTTTGCT TGGAAAGGCT 
CCGAGCAGCA GTGATATGCT GCTGAAACTG GCGCGCACCA CACCGTACTA CAAGAGGAAC 
AGACCACACA TCTGTTCCTT CTGGGTTAAA GGAGAGTGCA AAAGAGGAGA AGAGTGTCCG 
TACAGGCATG AAAAACCCAC TGATCCAGAT GACCCCCTGG CTGATCAGAA CATTAAGGAC 
CGATACTATG GAATTAATGA TCCTGTGGCT AATAAACTGC TGATGCGAGC CTCCACCATG 
CCCAGACTGG ACGTACCGGA CGACAAGTCC ATAACCACAC TTTATATTGG AGGCCTGGGA 
GAAAACGTCA CAGACTCGGA GCTCCGGAAT CACTTTTACC AGTTTGGAGA GATTCGGACC 
ATCACTATTG TCCAGAGGCA GCAGTGTGCT TTTATTCAGT TTGCCACTCG ACAGGCTGCA 
GAAACAGCTG CGGAGAAGTC CTTCAATAAA CTCATCATCA ATGGACGTCG TCTTAATGTT 
AAATGGGGCA GGTCCCAGGC GGCACGAGGG AAAGGAGAGA AGGACGGGGT GACGGAGAGT 
GGCATCAGAC TGGAGCCGGT GCCAGGGCTT CCTGGTGCTC TGCCTCCACC ACCTGTCTCT 
GATGAAGATG CGTCCACCAA TTACTTCAAT CTGGCTCCCA CACCCTCCCC TGCTGTCATG 
AACCTGGGTC TTCCTCCTCC ACCCGGGGTG ACCATGCCTC CTCCACCTGG ATTTGGTCCT 
CCTATGTTCC ACACTATGGA CCCCATGGCC CCGCCGGTGC CACCTCCAAT GGCCCTGAGA 
CCACCCGGAC AGGTCCACTA CCCATCTCAG GATCCTCAGA GGATGGGTGC CCACGCCAGT 
CGCCATGGAG GCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evolutionary plasticity of segmentation clock networks.
Development (Cambridge, England)138(13)2783-92(2011 Jul)
Krol AJ,Roellig D,Dequ?ant ML,Tassy O,Glynn E,Hattem G,Mushegian A,Oates AC,Pourqui? O

Spatiotemporal compartmentalization of key physiological processes during muscle precursor differentiation.
Proceedings of the National Academy of Sciences of the United States of America107(9)4224-9(2010 Mar)
Ozbudak EM,Tassy O,Pourqui? O

RNA-binding motif protein RBM22 is required for normal development of zebrafish embryos.
Genetics and molecular research : GMR8(4)1466-73(2009 Dec)
He F,Wang CT,Gou LT