pou1f1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pou1f1
Entrez Gene ID	405777
Full Name	POU class 1 homeobox 1
Synonyms	pit-1,pit1
General protein information	Preferred Names POU class 1 homeobox 1 Names pituitary-specific positive transcription factor 1 POU domain, class 1, transcription factor 1 pituitary-specific transcription factor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212851.1	NP_998016.1	pituitary-specific positive transcription factor 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	POU1F1	NP_001116229.1
Canis lupus familiaris (dog)	POU1F1	NP_001006950.1
Xenopus tropicalis (tropical clawed frog)	pou1f1	XP_002931806.1
Pan troglodytes (chimpanzee)	POU1F1	XP_003318250.1
	POU1F1	NP_001036325.1
Danio rerio (zebrafish)	pou1f1	NP_998016.1
Mus musculus (house mouse)	Pou1f1	NP_032875.1
Gallus gallus (chicken)	POU1F1	NP_989650.1
Bos taurus (cattle)	POU1F1	NP_777004.1
Rattus norvegicus (Norway rat)	Pou1f1	NP_037140.2

Gene Ontology
Bibliography

Related articles in PubMed

Integrated in silico and in vivo approaches to investigate effects of BDE-99 mediated by the nuclear receptors on developing zebrafish.
Zhang L, Jin Y, Han Z, Liu H, Shi L, Hua X, Doering JA, Tang S, Giesy JP, Yu H
Environmental toxicology and chemistry(2017 Oct)

greb1 regulates convergent extension movement and pituitary development in zebrafish.
Li SZ, Liu W, Li Z, Li WH, Wang Y, Zhou L, Gui JF
Gene627176-187(2017 Sep)

The combination of in silico and in?vivo approaches for the investigation of disrupting effects of tris (2-chloroethyl) phosphate (TCEP) toward core receptors of zebrafish.
Wu Y, Su G, Tang S, Liu W, Ma Z, Zheng X, Liu H, Yu H
Chemosphere168122-130(2017 Feb)

Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish.
Nakahara Y, Muto A, Hirabayashi R, Sakuma T, Yamamoto T, Kume S, Kikuchi Y
Genes to cells : devoted to molecular & cellular mechanisms21(5)492-504(2016 May)

The Severity of Acute Stress Is Represented by Increased Synchronous Activity and Recruitment of Hypothalamic CRH Neurons.
Vom Berg-Maurer CM, Trivedi CA, Bollmann JH, De Marco RJ, Ryu S
The Journal of neuroscience : the official journal of the Society for Neuroscience36(11)3350-62(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pou1f1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pou1f1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03528	NM_212851.1 Latest version!	Danio rerio POU class 1 homeobox 1 (pou1f1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pou1f1 ( NM_212851.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998016.1 Danio rerio POU class 1 homeobox 1 (pou1f1), mRNA.

Price & Availability↑

CloneID	ODa03528
Clone ID Related Accession (Same CDS sequence)	NM_212851.1
Accession Version	NM_212851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Danio rerio(zebrafish)
Product	pituitary-specific positive transcription factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY421970.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY421970.1, BC162244.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505391, SAMEA4476743 [ECO:0000350] ##Evidence-Data-END##

ATGAGCTGCC AGGCCTTCAG CACCAGCGAC TCCTTCAGCG GTTTAGGTGG AGACACTGGT 
GCTGCTCTCC CCCTGCTGAT GCACCACAGC GCCGCCGCAG ACTGCCACAA CACCAACATC 
TGCTCCACAG TCCCCTCAGG TCTCCCATTG GTCCAGTCGT CCAAGCGCTC TCATATGCAC 
CTGTCCACTT CTGCTTTGGG CAATGCGTCA GCCAGCCTCC ACTACCCGAT GCCCCCATGT 
CACTATAGCA ACCAGCAGAC CACCTACGGC ATGATGGCAG CACAGGAAAT GCTCTCTGCC 
AGCATCTCCC AGACCCGCAT CCTGCAGACC TGCAGTGTAC CTCATGCAAA CATGGTTAAC 
GGCGCCAACT CACTGCAAGG AGCTCTGGCT CCACGTCTCT ACAAGTTCCC TGAGCACAGT 
TTGGCTGGAG GATCCTGTGC TTTGAGCCAC GGCTTCGCTC CTCTTCCCCA GACCCTGCTA 
ACAGATGAGC CCATTCTAGG AGACATCAAG CAGGAGCTGC GCAGGAAGAG CCGGACCCTC 
GACGAGACCC CCGACATGGA CTCGCCTCAG ATCCGAGAGC TGGAGAAGTT CGCTAATGAC 
TTCAAATTAA GGAGGATCAA ACTTGGTTAC ACACAAACCA ATGTGGGTGA AGCTCTGGCT 
GCAGTGCACG GCTCCGAATT CAGCCAAACC ACAATCTGCC GCTTTGAGAA CCTGCAGCTC 
AGCTTTAAAA ACGCTTGCAA ACTCAAATCC ATCCTGGCGA AATGGCTGGA GGAGGCAGAG 
CAGGCCGGCG CTCTTTTTAA TGAGAAGATG GGTCTGCACG AGCGCAAGCG AAAGCGCAGA 
ACCACCATCA GTCTGGGGGC GAAGGAAGCA CTGGAGCGGA GCTTTGTGGA GAAGAGCAAG 
CCGTCGTCTC AAGAGATCGT GCGAATGGCC GAGGGTCTGC ATCTGGAGAA GGAGGTAGTC 
CGAGTCTGGT TCTGCAACCG CCGGCAGAGG GAGAAGAGGG TCAAAACCAG CCTACATCAC 
AGCTCCTTCA TGACCAAAGA CTGCAGAGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998016.1
CDS	27..1079
Translation	MSCQAFSTSDSFSGLGGDTGAALPLLMHHSAAADCHNTNICSTVPSGLPLVQSSKRSHMHLSTSALGNASASLHYPMPPCHYSNQQTTYGMMAAQEMLSASISQTRILQTCSVPHANMVNGANSLQGALAPRLYKFPEHSLAGGSCALSHGFAPLPQTLLTDEPILGDIKQELRRKSRTLDETPDMDSPQIRELEKFANDFKLRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSFKNACKLKSILAKWLEEAEQAGALFNEKMGLHERKRKRRTTISLGAKEALERSFVEKSKPSSQEIVRMAEGLHLEKEVVRVWFCNRRQREKRVKTSLHHSSFMTKDCRA

Target ORF information:

RefSeq Version	NM_212851.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio POU class 1 homeobox 1 (pou1f1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212851.1

ORF Insert Sequence:

ATGAGCTGCC AGGCCTTCAG CACCAGCGAC TCCTTCAGCG GTTTAGGTGG AGACACTGGT 
GCTGCTCTCC CCCTGCTGAT GCACCACAGC GCCGCCGCAG ACTGCCACAA CACCAACATC 
TGCTCCACAG TCCCCTCAGG TCTCCCATTG GTCCAGTCGT CCAAGCGCTC TCATATGCAC 
CTGTCCACTT CTGCTTTGGG CAATGCGTCA GCCAGCCTCC ACTACCCGAT GCCCCCATGT 
CACTATAGCA ACCAGCAGAC CACCTACGGC ATGATGGCAG CACAGGAAAT GCTCTCTGCC 
AGCATCTCCC AGACCCGCAT CCTGCAGACC TGCAGTGTAC CTCATGCAAA CATGGTTAAC 
GGCGCCAACT CACTGCAAGG AGCTCTGGCT CCACGTCTCT ACAAGTTCCC TGAGCACAGT 
TTGGCTGGAG GATCCTGTGC TTTGAGCCAC GGCTTCGCTC CTCTTCCCCA GACCCTGCTA 
ACAGATGAGC CCATTCTAGG AGACATCAAG CAGGAGCTGC GCAGGAAGAG CCGGACCCTC 
GACGAGACCC CCGACATGGA CTCGCCTCAG ATCCGAGAGC TGGAGAAGTT CGCTAATGAC 
TTCAAATTAA GGAGGATCAA ACTTGGTTAC ACACAAACCA ATGTGGGTGA AGCTCTGGCT 
GCAGTGCACG GCTCCGAATT CAGCCAAACC ACAATCTGCC GCTTTGAGAA CCTGCAGCTC 
AGCTTTAAAA ACGCTTGCAA ACTCAAATCC ATCCTGGCGA AATGGCTGGA GGAGGCAGAG 
CAGGCCGGCG CTCTTTTTAA TGAGAAGATG GGTCTGCACG AGCGCAAGCG AAAGCGCAGA 
ACCACCATCA GTCTGGGGGC GAAGGAAGCA CTGGAGCGGA GCTTTGTGGA GAAGAGCAAG 
CCGTCGTCTC AAGAGATCGT GCGAATGGCC GAGGGTCTGC ATCTGGAGAA GGAGGTAGTC 
CGAGTCTGGT TCTGCAACCG CCGGCAGAGG GAGAAGAGGG TCAAAACCAG CCTACATCAC 
AGCTCCTTCA TGACCAAAGA CTGCAGAGCC TGA