il1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	il1b
Entrez Gene ID	405770
Full Name	interleukin 1, beta
Synonyms	il1-b,zgc:111873
General protein information	Preferred Names interleukin 1, beta Names interleukin-1 beta interlukin-1 beta
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212844.2	NP_998009.2	interleukin-1 beta

Pathways from BioSystems
Gene Ontology

The following il1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01754	NM_212844.2 Latest version!	Danio rerio interleukin 1, beta (il1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il1b ( NM_212844.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998009.2 Danio rerio interleukin 1, beta (il1b), mRNA.

Price & Availability↑

CloneID	ODa01754
Clone ID Related Accession (Same CDS sequence)	NM_212844.2
Accession Version	NM_212844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	interleukin-1 beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AM941672.1. On May 14, 2013 this sequence version replaced NM_212844.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AM941672.1, BC098597.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGAAAGC AGAGGAACTT AACCAGCTCT GAAATGATGG CATGCGGGCA ATATGAAGTC 
ACCATAGCTC CAAAAAATTT GTGGGAGACA GACGGTGCTG TTTATTCAGA TTCTGATGAG 
ATGGACTGTT CAGATCCGCT TGCAATGAGC TACAGATGCG ACATGCATGA GGGCATCAGG 
CTGGAGATGT GGACTTCGCA GCACAAAATG AAGCAGTTGG TGAACGTCAT CATCGCCCTG 
AACAGAATGA AGCACATCAA ACCCCAATCC ACAGAGTTTG GAGAAAAAGA GGTGCTGGAC 
ATGCTCATGG CGAACGTCAT CCAAGAGCGT GAAGTGAACG TGGTGGATTC AGTGCCGTCT 
TACACTAAAA CCAAAAACGT CTTGCAATGC ACGATTTGCG ATCAGTATAA GAAGTCTCTG 
GTGCGCAGCG GCGGATCTCC ACATTTGCAG GCCGTCACAC TGAGAGCCGG AAGCAGCGAC 
TTGAAAGTGC GCTTCAGCAT GTCCACATAT GCGTCGCCCA GTGCTCCGGC TACTTCTGCT 
CAGCCTGTGT GTTTGGGAAT CTCCAAAAGT AACCTGTACC TGGCCTGCAG CCCCGCCGAA 
GGCTCCGCTC CACATCTCGT ACTCAAGGAG ATCAGCGGGT CTCTGGAGAC CATTAAAGCT 
GGAGATCCAA ACGGATACGA CCAGCTGCTG TTCTTCAGGA AGGAGACCGG CAGCTCCATA 
AACACCTTCG AGTCCGTCAA ATGTCCCGGT TGGTTTATCA GCACCGCGTA TGAAGATTCG 
CAGATGGTGG AGATGGACAG GAAAGACACC GAGCGCATCA TTAACTTCGA GCTGCAGGAT 
AAAGTGCGCA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998009.2
CDS	24..878
Translation	MRKQRNLTSSEMMACGQYEVTIAPKNLWETDGAVYSDSDEMDCSDPLAMSYRCDMHEGIRLEMWTSQHKMKQLVNVIIALNRMKHIKPQSTEFGEKEVLDMLMANVIQEREVNVVDSVPSYTKTKNVLQCTICDQYKKSLVRSGGSPHLQAVTLRAGSSDLKVRFSMSTYASPSAPATSAQPVCLGISKSNLYLACSPAEGSAPHLVLKEISGSLETIKAGDPNGYDQLLFFRKETGSSINTFESVKCPGWFISTAYEDSQMVEMDRKDTERIINFELQDKVRI

Target ORF information:

RefSeq Version	NM_212844.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interleukin 1, beta (il1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212844.2

ORF Insert Sequence:

ATGAGAAAGC AGAGGAACTT AACCAGCTCT GAAATGATGG CATGCGGGCA ATATGAAGTC 
ACCATAGCTC CAAAAAATTT GTGGGAGACA GACGGTGCTG TTTATTCAGA TTCTGATGAG 
ATGGACTGTT CAGATCCGCT TGCAATGAGC TACAGATGCG ACATGCATGA GGGCATCAGG 
CTGGAGATGT GGACTTCGCA GCACAAAATG AAGCAGTTGG TGAACGTCAT CATCGCCCTG 
AACAGAATGA AGCACATCAA ACCCCAATCC ACAGAGTTTG GAGAAAAAGA GGTGCTGGAC 
ATGCTCATGG CGAACGTCAT CCAAGAGCGT GAAGTGAACG TGGTGGATTC AGTGCCGTCT 
TACACTAAAA CCAAAAACGT CTTGCAATGC ACGATTTGCG ATCAGTATAA GAAGTCTCTG 
GTGCGCAGCG GCGGATCTCC ACATTTGCAG GCCGTCACAC TGAGAGCCGG AAGCAGCGAC 
TTGAAAGTGC GCTTCAGCAT GTCCACATAT GCGTCGCCCA GTGCTCCGGC TACTTCTGCT 
CAGCCTGTGT GTTTGGGAAT CTCCAAAAGT AACCTGTACC TGGCCTGCAG CCCCGCCGAA 
GGCTCCGCTC CACATCTCGT ACTCAAGGAG ATCAGCGGGT CTCTGGAGAC CATTAAAGCT 
GGAGATCCAA ACGGATACGA CCAGCTGCTG TTCTTCAGGA AGGAGACCGG CAGCTCCATA 
AACACCTTCG AGTCCGTCAA ATGTCCCGGT TGGTTTATCA GCACCGCGTA TGAAGATTCG 
CAGATGGTGG AGATGGACAG GAAAGACACC GAGCGCATCA TTAACTTCGA GCTGCAGGAT 
AAAGTGCGCA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.