gnmt cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gnmt
Entrez Gene ID	403338
Full Name	glycine N-methyltransferase
General protein information	Preferred Names glycine N-methyltransferase Names glycine N-methyltransferase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212816.1	NP_997981.1	glycine N-methyltransferase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNMT	XP_518481.1
Bos taurus (cattle)	GNMT	NP_001193045.1
Danio rerio (zebrafish)	gnmt	NP_997981.1
Drosophila melanogaster (fruit fly)	CG6188	NP_650223.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002198	XP_565619.1
Canis lupus familiaris (dog)	GNMT	XP_532140.1
Homo sapiens (human)	GNMT	NP_061833.1
	GNMT	XP_001089876.1
Mus musculus (house mouse)	Gnmt	NP_034451.1

Gene Ontology
Bibliography

Related articles in PubMed

Global DNA methylation in gonads of adult zebrafish Danio rerio under bisphenol A exposure.
Liu Y, Zhang Y, Tao S, Guan Y, Zhang T, Wang Z
Ecotoxicology and environmental safety130124-32(2016 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Partial depletion of yolk during zebrafish embryogenesis changes the dynamics of methionine cycle and metabolic genes.
Huang Y, Linsen SE
BMC genomics16427(2015 Jun)

Use of the zebrafish larvae as a model to study cigarette smoke condensate toxicity.
Ellis LD, Soo EC, Achenbach JC, Morash MG, Soanes KH
PloS one9(12)e115305(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gnmt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gnmt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35005	NM_212816.1 Latest version!	Danio rerio glycine N-methyltransferase (gnmt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gnmt ( NM_212816.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997981.1 Danio rerio glycine N-methyltransferase (gnmt), mRNA.

Price & Availability↑

CloneID	ODa35005
Clone ID Related Accession (Same CDS sequence)	NM_212816.1
Accession Version	NM_212816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	glycine N-methyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC062527.1. ##Evidence-Data-START## Transcript exon combination :: BC062527.1, EE704062.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGTGGACA GTGTTTACCG TACACGCTCT CTCGGTGTTG GTGCTGTTGG ACTTCCTGAT 
CAGTATGCCG ACGGCAAAGC AGCGAAAGTG TGGCAGCTGT ACATCGGAGA CACTCGGAGC 
AGGACAGAAG AGTACAAGAG CTGGGTGCTG GCCCTACTCA GGAAGCATGG GGTTCAGAGG 
GTGCTGGATG TAGCCTGCGG CACAGGGGTG GATTCCATCA TGTTGGTAGA AGAGGGATTC 
AGCGTGGTTA GTGTGGATGC TAGTGATAAA ATGCTGAAAT ATGCTCTTAA AGAGAGATGG 
GAGAGAAGAA AAGAGACAGC TTTTGATAAC TGGGTGATTG AGGAGGCCAA CTGGCTAACA 
TTACCAGAAG ATGTTAAGAA GCCTGGAGAT GGCTTTGATG CTGTAATCTG TCTTGGGAAC 
TCATTTGCTC ATTTACCAGA TTTTAAAGGG GACCAAAGTG ATCAGAAGCT CGCCTTGCAA 
AACATAGGCA GCATGGTGAA ACCTGGAGGG ATCCTAATCA TTGACCACCG CAACTATGAT 
TACATCATTG AGACTGGCCA AGTGCCTCAA GGAAAGAATA TTTACTATAA GAGTGACCTG 
AAACAGGACA TCTCTACCTC AGTACTGTGG GTTAATAGCA AGCCTCACAT GATCACACTG 
GACTACACAC TGGTGCTGCC TCAACCAGAG GGAGCTCAGA CTCCGCCTGA CACAAGTAAA 
TTCCGCCTGT CATACTACCC CCATCGTGTG GAGAATTTTA AGGAGATATT AAAAGCAGCG 
TTTTGGGGAA AATGCGAGCA CAATGTATAT GCCGACTTCA AGCAATACAT TGCAGGAGGA 
GAGCCACCCT GTTATTTCAT CCATGTTGTT AAAAAGACAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997981.1
CDS	78..962
Translation	MVDSVYRTRSLGVGAVGLPDQYADGKAAKVWQLYIGDTRSRTEEYKSWVLALLRKHGVQRVLDVACGTGVDSIMLVEEGFSVVSVDASDKMLKYALKERWERRKETAFDNWVIEEANWLTLPEDVKKPGDGFDAVICLGNSFAHLPDFKGDQSDQKLALQNIGSMVKPGGILIIDHRNYDYIIETGQVPQGKNIYYKSDLKQDISTSVLWVNSKPHMITLDYTLVLPQPEGAQTPPDTSKFRLSYYPHRVENFKEILKAAFWGKCEHNVYADFKQYIAGGEPPCYFIHVVKKTA

Target ORF information:

RefSeq Version	NM_212816.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glycine N-methyltransferase (gnmt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212816.1

ORF Insert Sequence:

ATGGTGGACA GTGTTTACCG TACACGCTCT CTCGGTGTTG GTGCTGTTGG ACTTCCTGAT 
CAGTATGCCG ACGGCAAAGC AGCGAAAGTG TGGCAGCTGT ACATCGGAGA CACTCGGAGC 
AGGACAGAAG AGTACAAGAG CTGGGTGCTG GCCCTACTCA GGAAGCATGG GGTTCAGAGG 
GTGCTGGATG TAGCCTGCGG CACAGGGGTG GATTCCATCA TGTTGGTAGA AGAGGGATTC 
AGCGTGGTTA GTGTGGATGC TAGTGATAAA ATGCTGAAAT ATGCTCTTAA AGAGAGATGG 
GAGAGAAGAA AAGAGACAGC TTTTGATAAC TGGGTGATTG AGGAGGCCAA CTGGCTAACA 
TTACCAGAAG ATGTTAAGAA GCCTGGAGAT GGCTTTGATG CTGTAATCTG TCTTGGGAAC 
TCATTTGCTC ATTTACCAGA TTTTAAAGGG GACCAAAGTG ATCAGAAGCT CGCCTTGCAA 
AACATAGGCA GCATGGTGAA ACCTGGAGGG ATCCTAATCA TTGACCACCG CAACTATGAT 
TACATCATTG AGACTGGCCA AGTGCCTCAA GGAAAGAATA TTTACTATAA GAGTGACCTG 
AAACAGGACA TCTCTACCTC AGTACTGTGG GTTAATAGCA AGCCTCACAT GATCACACTG 
GACTACACAC TGGTGCTGCC TCAACCAGAG GGAGCTCAGA CTCCGCCTGA CACAAGTAAA 
TTCCGCCTGT CATACTACCC CCATCGTGTG GAGAATTTTA AGGAGATATT AAAAGCAGCG 
TTTTGGGGAA AATGCGAGCA CAATGTATAT GCCGACTTCA AGCAATACAT TGCAGGAGGA 
GAGCCACCCT GTTATTTCAT CCATGTTGTT AAAAAGACAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Global DNA methylation in gonads of adult zebrafish Danio rerio under bisphenol A exposure.
Ecotoxicology and environmental safety130124-32(2016 Aug)
Liu Y,Zhang Y,Tao S,Guan Y,Zhang T,Wang Z

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Partial depletion of yolk during zebrafish embryogenesis changes the dynamics of methionine cycle and metabolic genes.
BMC genomics16427(2015 Jun)
Huang Y,Linsen SE

Use of the zebrafish larvae as a model to study cigarette smoke condensate toxicity.
PloS one9(12)e115305(2014)
Ellis LD,Soo EC,Achenbach JC,Morash MG,Soanes KH