irf9 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	irf9
Entrez Gene ID	403013
Full Name	interferon regulatory factor 9
Synonyms	ISGF3G,zgc:92097
General protein information	Preferred Names interferon regulatory factor 9 Names interferon regulatory factor 9
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005156059.3	XP_005156116.1	interferon regulatory factor 9 isoform X1
NM_205710.2	NP_991273.2	interferon regulatory factor 9
XM_005156059.4	XP_005156116.1	interferon regulatory factor 9 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	IRF9	XP_001167256.1
	LOC715335	XP_002808501.1
Rattus norvegicus (Norway rat)	Irf9	NP_001012041.1
Danio rerio (zebrafish)	irf9	NP_991273.1
Xenopus tropicalis (tropical clawed frog)	LOC100487158	XP_002939071.2
Canis lupus familiaris (dog)	IRF9	XP_860263.1
Mus musculus (house mouse)	Irf9	NP_032420.1
Homo sapiens (human)	IRF9	NP_006075.3
Bos taurus (cattle)	IRF9	NP_001019677.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of DNA methylation in zebrafish embryos activates retrotransposons to trigger antiviral signaling.
Chernyavskaya Y, Mudbhary R, Zhang C, Tokarz D, Jacob V, Gopinath S, Sun X, Wang S, Magnani E, Madakashira BP, Yoder JA, Hoshida Y, Sadler KC
Development (Cambridge, England)144(16)2925-2939(2017 08)

Histological and transcriptomic effects of 17?-methyltestosterone on zebrafish gonad development.
Lee SLJ, Horsfield JA, Black MA, Rutherford K, Fisher A, Gemmell NJ
BMC genomics18(1)557(2017 Jul)

IRF9-Stat2 Fusion Protein as an Innate Immune Inducer to Activate Mx and Interferon-Stimulated Gene Expression in Zebrafish Larvae.
Huang CJ, Chou CM, Lien HW, Chu CY, Ho JY, Wu Y, Cheng CH
Marine biotechnology (New York, N.Y.)19(3)310-319(2017 Jun)

FTR83, a Member of the Large Fish-Specific finTRIM Family, Triggers IFN Pathway and Counters Viral Infection.
Langevin C, Aleksejeva E, Houel A, Briolat V, Torhy C, Lunazzi A, Levraud JP, Boudinot P
Frontiers in immunology8617(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Much more significant induction of zebrafish IRF9 by zebrafish IFNgamma2.
Title: Expression regulation of zebrafish interferon regulatory factor 9 by promoter analysis.

The following irf9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irf9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17628	NM_205710.2 Latest version!	Danio rerio interferon regulatory factor 9 (irf9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa17628	XM_005156059.4 Latest version!	Danio rerio interferon regulatory factor 9 (irf9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa17628	XM_005156059.3	Danio rerio interferon regulatory factor 9 (irf9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

irf9 ( NM_205710.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991273.2 Danio rerio interferon regulatory factor 9 (irf9), mRNA.

Price & Availability↑

CloneID	ODa17628
Clone ID Related Accession (Same CDS sequence)	XM_005156059.3 , NM_205710.2 , XM_005156059.4
Accession Version	NM_205710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-24
Organism	Danio rerio(zebrafish)
Product	interferon regulatory factor 9
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH433167.1, EH504444.1, BX571950.6, EH462679.1 and EH459171.1. On or before Jun 20, 2015 this sequence version replaced XM_005156058.2, NM_205710.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQQ01003573.1, AY398365.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATCTG GAAGGATACG CTCCACACGC CGTCTGCGTT CATGGATAGT AGAACAGGTG 
AATAGTGGGA AGTATCTCGG CCTGGTTTGG GACAACCCAG AGAAAACCAT GTTTCGCATC 
CCATGGAAAC ATGCAGGAAA ACAAGACTTT CGCAGTGAGG AAGATGCAGC TATTTTCAAG 
GCTTGGGCGG CGTTTAAAGG GAAGCTCATG GAAAATGGAA ATTCGGACCC TGCGTCCTGG 
AAAACACGCC TTCGATGTGC CCTCAACAAA AGTCCAGAAT TTAGTGAGGT CACAGAAAGG 
TCACAGCTGG ATATTTCAGA ACCCTACAAG GTGTATCGCC TTGTACCACC AGAAGAACAA 
GGAGTGGTTA AAGTTAAAAA AGAGAATGGT CAGAAATCAG TGAGAAACAG CAAGAGGAAA 
CACAGCGAAT CTGAGAATGA ACAGGAGACT GAAGGCAAGA AGATCAAAGA GGAGATTGTT 
GCACCACAGG CCATCAACAT GAGTGCACAG GAAATCTTCA TAAATACAGG AAGTGCAATA 
ACAATTGAAA CAGGGATGGA GAAGAGTGAC AACAACACAG AAGATATCCA GTTAAACTTC 
ACCATTGAAG CTGTACCTCT GGTCGAAAAG AGAGCACTGC ACACTTTTCA CATAACCGTG 
CACTACATTG GTCAGGAGGT TTTGCGGCAA GAAGTAATGA GAAACGATGT CCGGATCTCT 
TACCTACCTC GCTCACCTGT TCCTCCATCT CTACCATCTC TCAGTGGCTC CAACTTCCAT 
CGAATCTTTC TCCCGGAACC TCCATCAGAT ATAAGCACAG ACTCTAGTCT CGCCCCACGC 
TTGAATGCTC TTAAATCCCT GCTACCCTTC ATGGAATGGG GAGTAGCGCT GACCTCCTTA 
TCAGGAGGCA TCTATGCCAA GCGCTTCTGC CAGGGACGGG TCTTCTGGAG AGGACCACAT 
AGTTCCACCA CAGGACCTTG TAAAATGGAA AGATCAGGCA CGCCAACCCA GCTGTTTAAC 
CGGGAGATAT TTCAACAAGA GTTGGAATGT TTCCGCAATG GAGGAAAACA ACCTCAAAGT 
GAGATCATTC TTTGTTTTGG AGAGGAGCTC TTGGAGGGAG ATAACATCTC TGATAAACAC 
ATCATCATTA AGATATCTCT CCCTTGGGCT GAGGCGCAGA TAGAAGAGGT CAAGGCCTTC 
AGAGAGTCCA TTGCAATTCT CAAAAGTCTG GCCAGCCAGT CTCCTACAGG GGAAGTGACA 
CTGAATTTTG TTGAAGTATC GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991273.2
CDS	130..1416
Translation	MASGRIRSTRRLRSWIVEQVNSGKYLGLVWDNPEKTMFRIPWKHAGKQDFRSEEDAAIFKAWAAFKGKLMENGNSDPASWKTRLRCALNKSPEFSEVTERSQLDISEPYKVYRLVPPEEQGVVKVKKENGQKSVRNSKRKHSESENEQETEGKKIKEEIVAPQAINMSAQEIFINTGSAITIETGMEKSDNNTEDIQLNFTIEAVPLVEKRALHTFHITVHYIGQEVLRQEVMRNDVRISYLPRSPVPPSLPSLSGSNFHRIFLPEPPSDISTDSSLAPRLNALKSLLPFMEWGVALTSLSGGIYAKRFCQGRVFWRGPHSSTTGPCKMERSGTPTQLFNREIFQQELECFRNGGKQPQSEIILCFGEELLEGDNISDKHIIIKISLPWAEAQIEEVKAFRESIAILKSLASQSPTGEVTLNFVEVSE

Target ORF information:

RefSeq Version	NM_205710.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interferon regulatory factor 9 (irf9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205710.2

ORF Insert Sequence:

ATGGCATCTG GAAGGATACG CTCCACACGC CGTCTGCGTT CATGGATAGT AGAACAGGTG 
AATAGTGGGA AGTATCTCGG CCTGGTTTGG GACAACCCAG AGAAAACCAT GTTTCGCATC 
CCATGGAAAC ATGCAGGAAA ACAAGACTTT CGCAGTGAGG AAGATGCAGC TATTTTCAAG 
GCTTGGGCGG CGTTTAAAGG GAAGCTCATG GAAAATGGAA ATTCGGACCC TGCGTCCTGG 
AAAACACGCC TTCGATGTGC CCTCAACAAA AGTCCAGAAT TTAGTGAGGT CACAGAAAGG 
TCACAGCTGG ATATTTCAGA ACCCTACAAG GTGTATCGCC TTGTACCACC AGAAGAACAA 
GGAGTGGTTA AAGTTAAAAA AGAGAATGGT CAGAAATCAG TGAGAAACAG CAAGAGGAAA 
CACAGCGAAT CTGAGAATGA ACAGGAGACT GAAGGCAAGA AGATCAAAGA GGAGATTGTT 
GCACCACAGG CCATCAACAT GAGTGCACAG GAAATCTTCA TAAATACAGG AAGTGCAATA 
ACAATTGAAA CAGGGATGGA GAAGAGTGAC AACAACACAG AAGATATCCA GTTAAACTTC 
ACCATTGAAG CTGTACCTCT GGTCGAAAAG AGAGCACTGC ACACTTTTCA CATAACCGTG 
CACTACATTG GTCAGGAGGT TTTGCGGCAA GAAGTAATGA GAAACGATGT CCGGATCTCT 
TACCTACCTC GCTCACCTGT TCCTCCATCT CTACCATCTC TCAGTGGCTC CAACTTCCAT 
CGAATCTTTC TCCCGGAACC TCCATCAGAT ATAAGCACAG ACTCTAGTCT CGCCCCACGC 
TTGAATGCTC TTAAATCCCT GCTACCCTTC ATGGAATGGG GAGTAGCGCT GACCTCCTTA 
TCAGGAGGCA TCTATGCCAA GCGCTTCTGC CAGGGACGGG TCTTCTGGAG AGGACCACAT 
AGTTCCACCA CAGGACCTTG TAAAATGGAA AGATCAGGCA CGCCAACCCA GCTGTTTAAC 
CGGGAGATAT TTCAACAAGA GTTGGAATGT TTCCGCAATG GAGGAAAACA ACCTCAAAGT 
GAGATCATTC TTTGTTTTGG AGAGGAGCTC TTGGAGGGAG ATAACATCTC TGATAAACAC 
ATCATCATTA AGATATCTCT CCCTTGGGCT GAGGCGCAGA TAGAAGAGGT CAAGGCCTTC 
AGAGAGTCCA TTGCAATTCT CAAAAGTCTG GCCAGCCAGT CTCCTACAGG GGAAGTGACA 
CTGAATTTTG TTGAAGTATC GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

irf9 ( XM_005156059.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156116.1 Danio rerio interferon regulatory factor 9 (irf9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17628
Clone ID Related Accession (Same CDS sequence)	XM_005156059.3 , NM_205710.2 , XM_005156059.4
Accession Version	XM_005156059.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	interferon regulatory factor 9 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156059.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTG GAAGGATACG CTCCACACGC CGTCTGCGTT CATGGATAGT AGAACAGGTG 
AATAGTGGGA AGTATCTCGG CCTGGTTTGG GACAACCCAG AGAAAACCAT GTTTCGCATC 
CCATGGAAAC ATGCAGGAAA ACAAGACTTT CGCAGTGAGG AAGATGCAGC TATTTTCAAG 
GCTTGGGCGG CGTTTAAAGG GAAGCTCATG GAAAATGGAA ATTCGGACCC TGCGTCCTGG 
AAAACACGCC TTCGATGTGC CCTCAACAAA AGTCCAGAAT TTAGTGAGGT CACAGAAAGG 
TCACAGCTGG ATATTTCAGA ACCCTACAAG GTGTATCGCC TTGTACCACC AGAAGAACAA 
GGAGTGGTTA AAGTTAAAAA AGAGAATGGT CAGAAATCAG TGAGAAACAG CAAGAGGAAA 
CACAGCGAAT CTGAGAATGA ACAGGAGACT GAAGGCAAGA AGATCAAAGA GGAGATTGTT 
GCACCACAGG CCATCAACAT GAGTGCACAG GAAATCTTCA TAAATACAGG AAGTGCAATA 
ACAATTGAAA CAGGGATGGA GAAGAGTGAC AACAACACAG AAGATATCCA GTTAAACTTC 
ACCATTGAAG CTGTACCTCT GGTCGAAAAG AGAGCACTGC ACACTTTTCA CATAACCGTG 
CACTACATTG GTCAGGAGGT TTTGCGGCAA GAAGTAATGA GAAACGATGT CCGGATCTCT 
TACCTACCTC GCTCACCTGT TCCTCCATCT CTACCATCTC TCAGTGGCTC CAACTTCCAT 
CGAATCTTTC TCCCGGAACC TCCATCAGAT ATAAGCACAG ACTCTAGTCT CGCCCCACGC 
TTGAATGCTC TTAAATCCCT GCTACCCTTC ATGGAATGGG GAGTAGCGCT GACCTCCTTA 
TCAGGAGGCA TCTATGCCAA GCGCTTCTGC CAGGGACGGG TCTTCTGGAG AGGACCACAT 
AGTTCCACCA CAGGACCTTG TAAAATGGAA AGATCAGGCA CGCCAACCCA GCTGTTTAAC 
CGGGAGATAT TTCAACAAGA GTTGGAATGT TTCCGCAATG GAGGAAAACA ACCTCAAAGT 
GAGATCATTC TTTGTTTTGG AGAGGAGCTC TTGGAGGGAG ATAACATCTC TGATAAACAC 
ATCATCATTA AGATATCTCT CCCTTGGGCT GAGGCGCAGA TAGAAGAGGT CAAGGCCTTC 
AGAGAGTCCA TTGCAATTCT CAAAAGTCTG GCCAGCCAGT CTCCTACAGG GGAAGTGACA 
CTGAATTTTG TTGAAGTATC GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156116.1
CDS	101..1387
Translation	MASGRIRSTRRLRSWIVEQVNSGKYLGLVWDNPEKTMFRIPWKHAGKQDFRSEEDAAIFKAWAAFKGKLMENGNSDPASWKTRLRCALNKSPEFSEVTERSQLDISEPYKVYRLVPPEEQGVVKVKKENGQKSVRNSKRKHSESENEQETEGKKIKEEIVAPQAINMSAQEIFINTGSAITIETGMEKSDNNTEDIQLNFTIEAVPLVEKRALHTFHITVHYIGQEVLRQEVMRNDVRISYLPRSPVPPSLPSLSGSNFHRIFLPEPPSDISTDSSLAPRLNALKSLLPFMEWGVALTSLSGGIYAKRFCQGRVFWRGPHSSTTGPCKMERSGTPTQLFNREIFQQELECFRNGGKQPQSEIILCFGEELLEGDNISDKHIIIKISLPWAEAQIEEVKAFRESIAILKSLASQSPTGEVTLNFVEVSE

Target ORF information:

RefSeq Version	XM_005156059.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio interferon regulatory factor 9 (irf9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156059.3

ORF Insert Sequence:

ATGGCATCTG GAAGGATACG CTCCACACGC CGTCTGCGTT CATGGATAGT AGAACAGGTG 
AATAGTGGGA AGTATCTCGG CCTGGTTTGG GACAACCCAG AGAAAACCAT GTTTCGCATC 
CCATGGAAAC ATGCAGGAAA ACAAGACTTT CGCAGTGAGG AAGATGCAGC TATTTTCAAG 
GCTTGGGCGG CGTTTAAAGG GAAGCTCATG GAAAATGGAA ATTCGGACCC TGCGTCCTGG 
AAAACACGCC TTCGATGTGC CCTCAACAAA AGTCCAGAAT TTAGTGAGGT CACAGAAAGG 
TCACAGCTGG ATATTTCAGA ACCCTACAAG GTGTATCGCC TTGTACCACC AGAAGAACAA 
GGAGTGGTTA AAGTTAAAAA AGAGAATGGT CAGAAATCAG TGAGAAACAG CAAGAGGAAA 
CACAGCGAAT CTGAGAATGA ACAGGAGACT GAAGGCAAGA AGATCAAAGA GGAGATTGTT 
GCACCACAGG CCATCAACAT GAGTGCACAG GAAATCTTCA TAAATACAGG AAGTGCAATA 
ACAATTGAAA CAGGGATGGA GAAGAGTGAC AACAACACAG AAGATATCCA GTTAAACTTC 
ACCATTGAAG CTGTACCTCT GGTCGAAAAG AGAGCACTGC ACACTTTTCA CATAACCGTG 
CACTACATTG GTCAGGAGGT TTTGCGGCAA GAAGTAATGA GAAACGATGT CCGGATCTCT 
TACCTACCTC GCTCACCTGT TCCTCCATCT CTACCATCTC TCAGTGGCTC CAACTTCCAT 
CGAATCTTTC TCCCGGAACC TCCATCAGAT ATAAGCACAG ACTCTAGTCT CGCCCCACGC 
TTGAATGCTC TTAAATCCCT GCTACCCTTC ATGGAATGGG GAGTAGCGCT GACCTCCTTA 
TCAGGAGGCA TCTATGCCAA GCGCTTCTGC CAGGGACGGG TCTTCTGGAG AGGACCACAT 
AGTTCCACCA CAGGACCTTG TAAAATGGAA AGATCAGGCA CGCCAACCCA GCTGTTTAAC 
CGGGAGATAT TTCAACAAGA GTTGGAATGT TTCCGCAATG GAGGAAAACA ACCTCAAAGT 
GAGATCATTC TTTGTTTTGG AGAGGAGCTC TTGGAGGGAG ATAACATCTC TGATAAACAC 
ATCATCATTA AGATATCTCT CCCTTGGGCT GAGGCGCAGA TAGAAGAGGT CAAGGCCTTC 
AGAGAGTCCA TTGCAATTCT CAAAAGTCTG GCCAGCCAGT CTCCTACAGG GGAAGTGACA 
CTGAATTTTG TTGAAGTATC GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

irf9 ( XM_005156059.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156116.1 Danio rerio interferon regulatory factor 9 (irf9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17628
Clone ID Related Accession (Same CDS sequence)	XM_005156059.3 , NM_205710.2 , XM_005156059.4
Accession Version	XM_005156059.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	interferon regulatory factor 9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156059.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTG GAAGGATACG CTCCACACGC CGTCTGCGTT CATGGATAGT AGAACAGGTG 
AATAGTGGGA AGTATCTCGG CCTGGTTTGG GACAACCCAG AGAAAACCAT GTTTCGCATC 
CCATGGAAAC ATGCAGGAAA ACAAGACTTT CGCAGTGAGG AAGATGCAGC TATTTTCAAG 
GCTTGGGCGG CGTTTAAAGG GAAGCTCATG GAAAATGGAA ATTCGGACCC TGCGTCCTGG 
AAAACACGCC TTCGATGTGC CCTCAACAAA AGTCCAGAAT TTAGTGAGGT CACAGAAAGG 
TCACAGCTGG ATATTTCAGA ACCCTACAAG GTGTATCGCC TTGTACCACC AGAAGAACAA 
GGAGTGGTTA AAGTTAAAAA AGAGAATGGT CAGAAATCAG TGAGAAACAG CAAGAGGAAA 
CACAGCGAAT CTGAGAATGA ACAGGAGACT GAAGGCAAGA AGATCAAAGA GGAGATTGTT 
GCACCACAGG CCATCAACAT GAGTGCACAG GAAATCTTCA TAAATACAGG AAGTGCAATA 
ACAATTGAAA CAGGGATGGA GAAGAGTGAC AACAACACAG AAGATATCCA GTTAAACTTC 
ACCATTGAAG CTGTACCTCT GGTCGAAAAG AGAGCACTGC ACACTTTTCA CATAACCGTG 
CACTACATTG GTCAGGAGGT TTTGCGGCAA GAAGTAATGA GAAACGATGT CCGGATCTCT 
TACCTACCTC GCTCACCTGT TCCTCCATCT CTACCATCTC TCAGTGGCTC CAACTTCCAT 
CGAATCTTTC TCCCGGAACC TCCATCAGAT ATAAGCACAG ACTCTAGTCT CGCCCCACGC 
TTGAATGCTC TTAAATCCCT GCTACCCTTC ATGGAATGGG GAGTAGCGCT GACCTCCTTA 
TCAGGAGGCA TCTATGCCAA GCGCTTCTGC CAGGGACGGG TCTTCTGGAG AGGACCACAT 
AGTTCCACCA CAGGACCTTG TAAAATGGAA AGATCAGGCA CGCCAACCCA GCTGTTTAAC 
CGGGAGATAT TTCAACAAGA GTTGGAATGT TTCCGCAATG GAGGAAAACA ACCTCAAAGT 
GAGATCATTC TTTGTTTTGG AGAGGAGCTC TTGGAGGGAG ATAACATCTC TGATAAACAC 
ATCATCATTA AGATATCTCT CCCTTGGGCT GAGGCGCAGA TAGAAGAGGT CAAGGCCTTC 
AGAGAGTCCA TTGCAATTCT CAAAAGTCTG GCCAGCCAGT CTCCTACAGG GGAAGTGACA 
CTGAATTTTG TTGAAGTATC GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156116.1
CDS	136..1422
Translation	MASGRIRSTRRLRSWIVEQVNSGKYLGLVWDNPEKTMFRIPWKHAGKQDFRSEEDAAIFKAWAAFKGKLMENGNSDPASWKTRLRCALNKSPEFSEVTERSQLDISEPYKVYRLVPPEEQGVVKVKKENGQKSVRNSKRKHSESENEQETEGKKIKEEIVAPQAINMSAQEIFINTGSAITIETGMEKSDNNTEDIQLNFTIEAVPLVEKRALHTFHITVHYIGQEVLRQEVMRNDVRISYLPRSPVPPSLPSLSGSNFHRIFLPEPPSDISTDSSLAPRLNALKSLLPFMEWGVALTSLSGGIYAKRFCQGRVFWRGPHSSTTGPCKMERSGTPTQLFNREIFQQELECFRNGGKQPQSEIILCFGEELLEGDNISDKHIIIKISLPWAEAQIEEVKAFRESIAILKSLASQSPTGEVTLNFVEVSE

Target ORF information:

RefSeq Version	XM_005156059.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interferon regulatory factor 9 (irf9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156059.4

ORF Insert Sequence:

ATGGCATCTG GAAGGATACG CTCCACACGC CGTCTGCGTT CATGGATAGT AGAACAGGTG 
AATAGTGGGA AGTATCTCGG CCTGGTTTGG GACAACCCAG AGAAAACCAT GTTTCGCATC 
CCATGGAAAC ATGCAGGAAA ACAAGACTTT CGCAGTGAGG AAGATGCAGC TATTTTCAAG 
GCTTGGGCGG CGTTTAAAGG GAAGCTCATG GAAAATGGAA ATTCGGACCC TGCGTCCTGG 
AAAACACGCC TTCGATGTGC CCTCAACAAA AGTCCAGAAT TTAGTGAGGT CACAGAAAGG 
TCACAGCTGG ATATTTCAGA ACCCTACAAG GTGTATCGCC TTGTACCACC AGAAGAACAA 
GGAGTGGTTA AAGTTAAAAA AGAGAATGGT CAGAAATCAG TGAGAAACAG CAAGAGGAAA 
CACAGCGAAT CTGAGAATGA ACAGGAGACT GAAGGCAAGA AGATCAAAGA GGAGATTGTT 
GCACCACAGG CCATCAACAT GAGTGCACAG GAAATCTTCA TAAATACAGG AAGTGCAATA 
ACAATTGAAA CAGGGATGGA GAAGAGTGAC AACAACACAG AAGATATCCA GTTAAACTTC 
ACCATTGAAG CTGTACCTCT GGTCGAAAAG AGAGCACTGC ACACTTTTCA CATAACCGTG 
CACTACATTG GTCAGGAGGT TTTGCGGCAA GAAGTAATGA GAAACGATGT CCGGATCTCT 
TACCTACCTC GCTCACCTGT TCCTCCATCT CTACCATCTC TCAGTGGCTC CAACTTCCAT 
CGAATCTTTC TCCCGGAACC TCCATCAGAT ATAAGCACAG ACTCTAGTCT CGCCCCACGC 
TTGAATGCTC TTAAATCCCT GCTACCCTTC ATGGAATGGG GAGTAGCGCT GACCTCCTTA 
TCAGGAGGCA TCTATGCCAA GCGCTTCTGC CAGGGACGGG TCTTCTGGAG AGGACCACAT 
AGTTCCACCA CAGGACCTTG TAAAATGGAA AGATCAGGCA CGCCAACCCA GCTGTTTAAC 
CGGGAGATAT TTCAACAAGA GTTGGAATGT TTCCGCAATG GAGGAAAACA ACCTCAAAGT 
GAGATCATTC TTTGTTTTGG AGAGGAGCTC TTGGAGGGAG ATAACATCTC TGATAAACAC 
ATCATCATTA AGATATCTCT CCCTTGGGCT GAGGCGCAGA TAGAAGAGGT CAAGGCCTTC 
AGAGAGTCCA TTGCAATTCT CAAAAGTCTG GCCAGCCAGT CTCCTACAGG GGAAGTGACA 
CTGAATTTTG TTGAAGTATC GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of DNA methylation in zebrafish embryos activates retrotransposons to trigger antiviral signaling.
Development (Cambridge, England)144(16)2925-2939(2017 08)
Chernyavskaya Y,Mudbhary R,Zhang C,Tokarz D,Jacob V,Gopinath S,Sun X,Wang S,Magnani E,Madakashira BP,Yoder JA,Hoshida Y,Sadler KC

Histological and transcriptomic effects of 17?-methyltestosterone on zebrafish gonad development.
BMC genomics18(1)557(2017 Jul)
Lee SLJ,Horsfield JA,Black MA,Rutherford K,Fisher A,Gemmell NJ

IRF9-Stat2 Fusion Protein as an Innate Immune Inducer to Activate Mx and Interferon-Stimulated Gene Expression in Zebrafish Larvae.
Marine biotechnology (New York, N.Y.)19(3)310-319(2017 Jun)
Huang CJ,Chou CM,Lien HW,Chu CY,Ho JY,Wu Y,Cheng CH

FTR83, a Member of the Large Fish-Specific finTRIM Family, Triggers IFN Pathway and Counters Viral Infection.
Frontiers in immunology8617(2017)
Langevin C,Aleksejeva E,Houel A,Briolat V,Torhy C,Lunazzi A,Levraud JP,Boudinot P