xrn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	xrn1
Entrez Gene ID	394008
Full Name	5'-3' exoribonuclease 1
Synonyms	wu:fk92c07,zgc:63635
General protein information	Preferred Names 5'-3' exoribonuclease 1 Names 5'-3' exoribonuclease 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005163317.4	XP_005163374.1	5'-3' exoribonuclease 1 isoform X1
XM_005163317.3	XP_005163374.1	5'-3' exoribonuclease 1 isoform X1
XM_005163318.3	XP_005163375.1	5'-3' exoribonuclease 1 isoform X2
NM_201033.2	NP_957327.2	5'-3' exoribonuclease 1
XM_005163318.4	XP_005163375.1	5'-3' exoribonuclease 1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	XRN1	NP_061874.3
Mus musculus (house mouse)	Xrn1	NP_036046.2
Rattus norvegicus (Norway rat)	Xrn1	XP_217233.5
Caenorhabditis elegans (roundworm)	xrn-1	NP_496945.3
Schizosaccharomyces pombe (fission yeast)	exo2	NP_593482.1
	XRN1	XP_001112305.1
Saccharomyces cerevisiae (baker's yeast)	XRN1	NP_011342.1
Magnaporthe oryzae (rice blast fungus)	MGG_12403	XP_003715558.1
Canis lupus familiaris (dog)	XRN1	XP_005634594.1
Danio rerio (zebrafish)	xrn1	NP_957327.2
Drosophila melanogaster (fruit fly)	pcm	NP_001162796.1
Eremothecium gossypii	AGOS_ADL046C	NP_984050.1
Neurospora crassa	NCU06678	XP_960925.2
Gallus gallus (chicken)	XRN1	XP_422596.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004430	XP_313715.5
Xenopus tropicalis (tropical clawed frog)	xrn1	XP_002937144.2
Pan troglodytes (chimpanzee)	XRN1	XP_001161640.1
Bos taurus (cattle)	XRN1	NP_001192964.1
Kluyveromyces lactis	KLLA0F22385g	XP_456080.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequential, Divergent, and Cooperative Requirements of
Yang YJ, Wang Y, Li Z, Zhou L, Gui JF
Genetics205(4)1551-1572(2017 04)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following xrn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the xrn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35129	NM_201033.2 Latest version!	Danio rerio 5'-3' exoribonuclease 1 (xrn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa06835	XM_005163317.4 Latest version!	Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa06836	XM_005163318.4 Latest version!	Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa06835	XM_005163317.3	Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa06836	XM_005163318.3	Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

xrn1 ( NM_201033.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957327.2 Danio rerio 5'-3' exoribonuclease 1 (xrn1), mRNA.

Price & Availability↑

CloneID	ODa35129
Clone ID Related Accession (Same CDS sequence)	NM_201033.2
Accession Version	NM_201033.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	5'-3' exoribonuclease 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU929319.6. On or before Oct 27, 2010 this sequence version replaced XM_001920726.2, NM_201033.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGTGC CTAAGTTTTA TCGATGGATA TCAGAGCGGT ATCCGTGTCT GAGTGAAGTT 
GTCAAAGAGC ATCAGATCCC AGAGTTCGAC AACCTGTATT TAGACATGAA CGGGATCATC 
CACCAGTGTT CGCACCCAAA TGATGAGGAT GTGCACTTCC GTATTTCTGA AGAGAAGATA 
TTCGCTGACA TTTTTCACTA TCTGGAAGTG CTCTTTCGCA TCATTAAGCC AAGGAAAGTG 
TTCTTCATGG CCGTCGATGG AGTCGCACCC AGAGCCAAAA TGAACCAGCA GCGTGGGAGA 
AGATTCAGGT CAGCAAAAGA GGCAGAGGAG AAAATCAAGA AGGCCTTGGA GAAGGGAGAA 
GTTTTGCCCA CAGAGGCCCG ATTTGACTCT AACTGCATCA CTCCGGGAAC GGATTTCATG 
GCCAGGCTAC AGGAACAGCT TAAATATTTC GTTCACAATA AACTGTCCAC AGACAAAACA 
TGGCAGGGAG TGAATGTGTA TTTGTCTGGT CATGAGACCC CTGGTGAAGG AGAGCACAAA 
ATCATGGAGT TCATTCGCTC TGAGAGCACC AAACCGGGTC ATAACCCAAA CACAAGACAC 
TGCCTTTATG GCCTGGATGC CGATCTGATA ATGCTTGGCT TGACCAGCCA TGAGCCTCAT 
TTTTCTCTCC TCAGAGAGGA GGTTCGCTTT GGAGGCAAAA AGTCACAAAA GAGGATAACA 
GCTCCTGAGG AGACGACCTT TCATCTGCTT CATCTCTCCC TTATGAGGGA ATACATTGAC 
TATGAGTTTT CAGAGCTCAG GAAAAAGATC TCATTTGAGT ATGACCTGGA GCGGATCATA 
GACGACTGGG TTCTGATGGG TTTCCTGGTG GGGAATGACT TCATCCCTCA TCTTCCTCAC 
CTGCACATCA ATCATGACGC ACTCCCGCTG CTCTATCGCA CCTACATTAA TGTGCTGCCA 
AGTCTAGGAG GATACCTGAA TGAAAACGGA CACCTCAACC TCACCCACTT TGAGAAGTAC 
CTAGAGAAGT TATCAGATTT TGACCGGGAG CACTTCAGTG AGGTGTTTGT GGACCTGAAG 
TGGTTTGAGA GTAAGGTGGG GAACAAATAT GTGAACGAAG CTGCGGGGCT GGCAGCTGAG 
GAGGCTGATG GCAGAGACTT GAAGAAGAGT AAATCTTCAG ACGACTCTCT GTGTCTCGCG 
GCTCTTGATG GAGTCAGAGA TTCTCACAGC ACTGCAGGAA GAGCAGAAGG CGCAGAGGAG 
GACGGAGAGG ACGATGACAT GTTTGAAACA GAATTTCGAC AATATAAACG CACCTACTAC 
ATGACCAAAA TGGGTGTTGA AGTCGTCTCA GATGAGTTTT TGGCCAATCA GGCTAAATGT 
TACGTAGAGG GAATCCAGTG GATCTTGCAT TACTACTATC ACGGAGTTCA GTCTTGGAGC 
TGGTATTATC CATACCACTA CGCTCCTTTC CTCTCTGACG TGAGGAACAT CTCTGGTCTC 
TCGCTGACCT TTGACCTGGC CAAACCCTTT ATGCCGTTTG AGCAGCTCTT GGCCGTCCTG 
CCATCAGCCA GCAAAGACCT TCTTCCTCAG TGCTACCAGC ATCTGATGAC GTCACTGAGC 
TCCCCCATTG TCGAGTATTA CCCACAGGAC TTCAAAACTG ACCTGAACGG CAAACAGCAG 
GAGTGGGAAG CAGTGGTGCT CATCCCGTTC ATTGACGAGA AATGCTTGTT AGCAGCTATG 
GAGCCCTACA CTCATAATCT GACCCCATCG GAGAAGGCCA GGAACAGGCA TACGGAGTGT 
GCAGTTTACT GCTACGATAA AGACCTGGAT TATCCCTATA ATTCCCCAGA TCCGCAGATC 
TTCCCCAATA TCGTCCACTG CCATGCCAGG CAAACACCGG TCCCGATGGA TGCCTGGCGT 
GTATCACCTG ATCATGTGCG ATGCAGGATC GACAGAGGAG CGCTTTACTT CTGCGGCTTC 
CCCACATTAC AGCACATCCG TCATAAATTT TACAAGAAGA AAAGTGGAGT GATGGTGTTT 
CAACAAAGCA GCAGAGGAGA AAACATGATC CTGGATATTT TGCCAAGCCA TGAGATGGTG 
CAGGTGGAAG AGGTTGCAGC GCTGGTTCTT GGAAGATCTT TGTTTGTGAA CTGGCCACAT 
TTGATGGAAG CTCGGGTTGT AGCCGTTTCA GATGGAGAGA CAAAATTTTC ATTAGAGGAG 
CCTGCAGGAT CACAGGTGTT GTACATGGAA AACAATCCAC CACCCACTAA AGTCAGCCGT 
GTGACTGATA AAGAGCAGAA GGACTGGGTC AAAGAAGTGC AAGGCCTCAC AGAGCATTTT 
AGCAAGCGTA AAGGCATAGC AATCAATGAG ACGGATGTGT TGGTTTACGG CCAGCTGCTG 
ACAGGCAGGA AGTATGTCAT CGGTCAGAAC GGAAAAGTCC ATTTGGAGAA GCAGTTTGCC 
AAGCAGGTCC TGCCCTTTCC ATATCAGACC ATTGTGAAGG ATATCAAGGC CTTTGATTCC 
TCATTGGCGC GCTTCAAAAC ACTGGAGGAG CTGTTTCCTC TGGGAACCAC AGTCTTTATG 
GTTGGAAATC CATATTACGG TGCAATGGGA GAAGTGCAAG ACTCTAGTGA TGTCATCAGT 
GAGGGACGAG TCAGAGTGGT TTTCTCTGTA CCGTGTGAAC CTCAGCTCGA TGCCTTAATA 
CAGAATCAGC ATAAATACTC TGTGAAGTAC AGTCCCGGGT ATGTACTCGC CTCACGCCTC 
GGTATCACTG GCTACCTCGT CTCAAGGTTC TCTGGTAGCA TCTTCATCGG AAGAGGATCC 
AAGAAAAATC CACATGGAGA GCAGAAAGTC AACGTAGGCC TTAATCTCAA GTTCAACAAA 
AAGAACGAGG AAGTTCCTGG CTACACCAAG AGAACAGAGA AGGAGTGGCT CTACTCTGCT 
GCTGTTGAGG AACTAATAGC TGAATACCTA GAACGATTTT CAGAGGTGTT TGACCATGTA 
GCACGAAACA GCCACGATGA CATATTTTAC GAAGACGACA TCTGGCCGGG AGAGGATGAG 
AATGGAGTGG AAAAGGTCCA AGAGATCCAG GGCTGGTTGA AAACGCATCC AGTGAGCTCC 
ATCTCCAGAG CATCATGTGA TCTGCAGATC CTGGATGCTG GTATTGTGGA GAAGATTGAG 
GAGGAGCTGG AGAAAACGAA GGCAAAGAAG ATGAACAAGA AAGTGCGGGT CACCGTGAAG 
CCCCATCTGC TTTTTAGGCC TCTGGAGCAG CAGCACGGTG TTGTTCCAGA TCCTGATTCA 
GAATATCATC TGTTTGACCG TGTGGTCAAT GTGAGAGAGA GCTTTTCTGT TCCACTCGGC 
CTTCGAGGAA CCATCATCGG CATCAAAGGA GCTGACCGTG AGGCTGAAAT TCTATATGAG 
GTGATATTTG ATGAGGAGTT TGCTGGAGGA CTCACTATCA GGTGTTCTCC TGGCCGTGGG 
TATCGTTTGC CCCCTAGTGC CTTAATCAAC CTGAGCCATG GCAGCAGGAA GGAGCTGGGC 
TCACACAAAC TCACTGCCAT CGTCAAACCT CAACCCTCAT CTTCATCACA AATCTCAAAC 
AAGAGCCAGC TGGGTGGCCT TAATCACTCA CCACGCTCAC CGTTCATACC TACACAGCAG 
CAGAATGGCA GACAAAGTTT TAATCTCAGG GGTGACACTC AGGGCAACAG GAACTCGCCA 
CATAAAGCCC TCAACCAGAG AAACCAACAA AAGGGGTCTA GTAATGAGAA AGAGTTCAGT 
AATGTATGGC AGTCCCTTCA GAATTCAGCA ATGCCCCAGA AACCACCAGC CCACTGGCAA 
AATAATGCTG GTAAAAGCCA GATGCCACAT CAGAATCAGC TGGAAGGACA AAATAAACAG 
AGCCAAGGCA ACGACACATC CATCAAACTT GGTTTGGGTG GTAATATTAG ACTCCTGAAG 
AGAAATGAGG ATCTAAATAC CGCTCTGCAG GGGCCTTCAA ATAAGGCATC TACAGAGTTT 
GAGGAGCTTA TAGCCAATCT TAAAATCTCA AAGGATCCTG AACCTCCTCA GCATGAACCA 
CCTAAAGAGC CCAAGGCCCC GTCAGCAGAG CCTCTCTCCC CACAATCCTT TGCCATGAAG 
GGAACTCTAG TATTGAAAGA GATGTTGAAG ATCGACAGCT CCGGAGCAAG TTCCTCTGCC 
CCTGATTCTG GCGGCCCCAA TGCCCAGCAG CAGTGCAAGA GACGCTCCAG CAAGAAACTA 
GCTGCTCGGA TAAACACTCC TCAGCCTGAA ACAGCTCCGG CTGCTGCTGC TGCTCCTCCT 
CCTCCTCCTC CTCCTCATCC TCTGGCTGGT CAGCCACCCC TCCTGCCCAG CATGGCCACT 
GAACTAGGAC GGGTGTGCAT GGGGCTCCGG ATGGGACCTC CAGAGTTTGC CTTCCTCCGC 
AACAGACAGA GTCTGATCTT GTGTCAGGTG AAGTTGTCGA ATGGTCTGAT GGTTCACGGC 
CCTCAGTGTC AGTCAGAAAC CGAAGCTAAA GAGAAAGCTG CTCTCTTCAC CCTTCAACGA 
TTGAACTCGC TGGGCTCTTT CCCATTGCCT CCTCCCATAT TCCCTGGAGT CCAGCAGATG 
AGACCGCTTG CACCTCATCC CTCCATGTTC GGACAGCCAG GAGGTGGGTT TCTGATGCCC 
CCTCAAGGGT ACGGGCCTCT TCCCTGGGGG ATGCAGTTTC CCCATCAGGG GCAGCCGTTC 
TTTGGAGGAA CTTTTCCTGG AGCACGAGGC CCTGTGCCAT CAGCAGCCGG CGGATCCCAT 
AACCAGTTTG TTCCCCTTCA GGTGACCAAG AAACGAGTTT CAGGCAGAAC CAAGAACCAA 
GAATCAAATC AAGCTCAGAG TGGCTCACCC TATGGCGTTC AGACCCCTGC AGTGCCTCCA 
TCAGCCCCTA AAACTCCACC ATCCACCAAC GCCCACGAGC ATGCAGAAAA GCCCACCGCA 
ACTACACCCA AGACCCCCAA GCAGAATGCC AATCCCCACA CCCCCGGCTC CGCATCCAAG 
AGGAAACACC GGAAACTGGC AGTCAACTTT GAGGCTGCTA AGGTGACGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957327.2
CDS	104..5197
Translation	MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDEDVHFRISEEKIFADIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEEKIKKALEKGEVLPTEARFDSNCITPGTDFMARLQEQLKYFVHNKLSTDKTWQGVNVYLSGHETPGEGEHKIMEFIRSESTKPGHNPNTRHCLYGLDADLIMLGLTSHEPHFSLLREEVRFGGKKSQKRITAPEETTFHLLHLSLMREYIDYEFSELRKKISFEYDLERIIDDWVLMGFLVGNDFIPHLPHLHINHDALPLLYRTYINVLPSLGGYLNENGHLNLTHFEKYLEKLSDFDREHFSEVFVDLKWFESKVGNKYVNEAAGLAAEEADGRDLKKSKSSDDSLCLAALDGVRDSHSTAGRAEGAEEDGEDDDMFETEFRQYKRTYYMTKMGVEVVSDEFLANQAKCYVEGIQWILHYYYHGVQSWSWYYPYHYAPFLSDVRNISGLSLTFDLAKPFMPFEQLLAVLPSASKDLLPQCYQHLMTSLSSPIVEYYPQDFKTDLNGKQQEWEAVVLIPFIDEKCLLAAMEPYTHNLTPSEKARNRHTECAVYCYDKDLDYPYNSPDPQIFPNIVHCHARQTPVPMDAWRVSPDHVRCRIDRGALYFCGFPTLQHIRHKFYKKKSGVMVFQQSSRGENMILDILPSHEMVQVEEVAALVLGRSLFVNWPHLMEARVVAVSDGETKFSLEEPAGSQVLYMENNPPPTKVSRVTDKEQKDWVKEVQGLTEHFSKRKGIAINETDVLVYGQLLTGRKYVIGQNGKVHLEKQFAKQVLPFPYQTIVKDIKAFDSSLARFKTLEELFPLGTTVFMVGNPYYGAMGEVQDSSDVISEGRVRVVFSVPCEPQLDALIQNQHKYSVKYSPGYVLASRLGITGYLVSRFSGSIFIGRGSKKNPHGEQKVNVGLNLKFNKKNEEVPGYTKRTEKEWLYSAAVEELIAEYLERFSEVFDHVARNSHDDIFYEDDIWPGEDENGVEKVQEIQGWLKTHPVSSISRASCDLQILDAGIVEKIEEELEKTKAKKMNKKVRVTVKPHLLFRPLEQQHGVVPDPDSEYHLFDRVVNVRESFSVPLGLRGTIIGIKGADREAEILYEVIFDEEFAGGLTIRCSPGRGYRLPPSALINLSHGSRKELGSHKLTAIVKPQPSSSSQISNKSQLGGLNHSPRSPFIPTQQQNGRQSFNLRGDTQGNRNSPHKALNQRNQQKGSSNEKEFSNVWQSLQNSAMPQKPPAHWQNNAGKSQMPHQNQLEGQNKQSQGNDTSIKLGLGGNIRLLKRNEDLNTALQGPSNKASTEFEELIANLKISKDPEPPQHEPPKEPKAPSAEPLSPQSFAMKGTLVLKEMLKIDSSGASSSAPDSGGPNAQQQCKRRSSKKLAARINTPQPETAPAAAAAPPPPPPPHPLAGQPPLLPSMATELGRVCMGLRMGPPEFAFLRNRQSLILCQVKLSNGLMVHGPQCQSETEAKEKAALFTLQRLNSLGSFPLPPPIFPGVQQMRPLAPHPSMFGQPGGGFLMPPQGYGPLPWGMQFPHQGQPFFGGTFPGARGPVPSAAGGSHNQFVPLQVTKKRVSGRTKNQESNQAQSGSPYGVQTPAVPPSAPKTPPSTNAHEHAEKPTATTPKTPKQNANPHTPGSASKRKHRKLAVNFEAAKVTD

Target ORF information:

RefSeq Version	NM_201033.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 5'-3' exoribonuclease 1 (xrn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201033.2

ORF Insert Sequence:

ATGGGAGTGC CTAAGTTTTA TCGATGGATA TCAGAGCGGT ATCCGTGTCT GAGTGAAGTT 
GTCAAAGAGC ATCAGATCCC AGAGTTCGAC AACCTGTATT TAGACATGAA CGGGATCATC 
CACCAGTGTT CGCACCCAAA TGATGAGGAT GTGCACTTCC GTATTTCTGA AGAGAAGATA 
TTCGCTGACA TTTTTCACTA TCTGGAAGTG CTCTTTCGCA TCATTAAGCC AAGGAAAGTG 
TTCTTCATGG CCGTCGATGG AGTCGCACCC AGAGCCAAAA TGAACCAGCA GCGTGGGAGA 
AGATTCAGGT CAGCAAAAGA GGCAGAGGAG AAAATCAAGA AGGCCTTGGA GAAGGGAGAA 
GTTTTGCCCA CAGAGGCCCG ATTTGACTCT AACTGCATCA CTCCGGGAAC GGATTTCATG 
GCCAGGCTAC AGGAACAGCT TAAATATTTC GTTCACAATA AACTGTCCAC AGACAAAACA 
TGGCAGGGAG TGAATGTGTA TTTGTCTGGT CATGAGACCC CTGGTGAAGG AGAGCACAAA 
ATCATGGAGT TCATTCGCTC TGAGAGCACC AAACCGGGTC ATAACCCAAA CACAAGACAC 
TGCCTTTATG GCCTGGATGC CGATCTGATA ATGCTTGGCT TGACCAGCCA TGAGCCTCAT 
TTTTCTCTCC TCAGAGAGGA GGTTCGCTTT GGAGGCAAAA AGTCACAAAA GAGGATAACA 
GCTCCTGAGG AGACGACCTT TCATCTGCTT CATCTCTCCC TTATGAGGGA ATACATTGAC 
TATGAGTTTT CAGAGCTCAG GAAAAAGATC TCATTTGAGT ATGACCTGGA GCGGATCATA 
GACGACTGGG TTCTGATGGG TTTCCTGGTG GGGAATGACT TCATCCCTCA TCTTCCTCAC 
CTGCACATCA ATCATGACGC ACTCCCGCTG CTCTATCGCA CCTACATTAA TGTGCTGCCA 
AGTCTAGGAG GATACCTGAA TGAAAACGGA CACCTCAACC TCACCCACTT TGAGAAGTAC 
CTAGAGAAGT TATCAGATTT TGACCGGGAG CACTTCAGTG AGGTGTTTGT GGACCTGAAG 
TGGTTTGAGA GTAAGGTGGG GAACAAATAT GTGAACGAAG CTGCGGGGCT GGCAGCTGAG 
GAGGCTGATG GCAGAGACTT GAAGAAGAGT AAATCTTCAG ACGACTCTCT GTGTCTCGCG 
GCTCTTGATG GAGTCAGAGA TTCTCACAGC ACTGCAGGAA GAGCAGAAGG CGCAGAGGAG 
GACGGAGAGG ACGATGACAT GTTTGAAACA GAATTTCGAC AATATAAACG CACCTACTAC 
ATGACCAAAA TGGGTGTTGA AGTCGTCTCA GATGAGTTTT TGGCCAATCA GGCTAAATGT 
TACGTAGAGG GAATCCAGTG GATCTTGCAT TACTACTATC ACGGAGTTCA GTCTTGGAGC 
TGGTATTATC CATACCACTA CGCTCCTTTC CTCTCTGACG TGAGGAACAT CTCTGGTCTC 
TCGCTGACCT TTGACCTGGC CAAACCCTTT ATGCCGTTTG AGCAGCTCTT GGCCGTCCTG 
CCATCAGCCA GCAAAGACCT TCTTCCTCAG TGCTACCAGC ATCTGATGAC GTCACTGAGC 
TCCCCCATTG TCGAGTATTA CCCACAGGAC TTCAAAACTG ACCTGAACGG CAAACAGCAG 
GAGTGGGAAG CAGTGGTGCT CATCCCGTTC ATTGACGAGA AATGCTTGTT AGCAGCTATG 
GAGCCCTACA CTCATAATCT GACCCCATCG GAGAAGGCCA GGAACAGGCA TACGGAGTGT 
GCAGTTTACT GCTACGATAA AGACCTGGAT TATCCCTATA ATTCCCCAGA TCCGCAGATC 
TTCCCCAATA TCGTCCACTG CCATGCCAGG CAAACACCGG TCCCGATGGA TGCCTGGCGT 
GTATCACCTG ATCATGTGCG ATGCAGGATC GACAGAGGAG CGCTTTACTT CTGCGGCTTC 
CCCACATTAC AGCACATCCG TCATAAATTT TACAAGAAGA AAAGTGGAGT GATGGTGTTT 
CAACAAAGCA GCAGAGGAGA AAACATGATC CTGGATATTT TGCCAAGCCA TGAGATGGTG 
CAGGTGGAAG AGGTTGCAGC GCTGGTTCTT GGAAGATCTT TGTTTGTGAA CTGGCCACAT 
TTGATGGAAG CTCGGGTTGT AGCCGTTTCA GATGGAGAGA CAAAATTTTC ATTAGAGGAG 
CCTGCAGGAT CACAGGTGTT GTACATGGAA AACAATCCAC CACCCACTAA AGTCAGCCGT 
GTGACTGATA AAGAGCAGAA GGACTGGGTC AAAGAAGTGC AAGGCCTCAC AGAGCATTTT 
AGCAAGCGTA AAGGCATAGC AATCAATGAG ACGGATGTGT TGGTTTACGG CCAGCTGCTG 
ACAGGCAGGA AGTATGTCAT CGGTCAGAAC GGAAAAGTCC ATTTGGAGAA GCAGTTTGCC 
AAGCAGGTCC TGCCCTTTCC ATATCAGACC ATTGTGAAGG ATATCAAGGC CTTTGATTCC 
TCATTGGCGC GCTTCAAAAC ACTGGAGGAG CTGTTTCCTC TGGGAACCAC AGTCTTTATG 
GTTGGAAATC CATATTACGG TGCAATGGGA GAAGTGCAAG ACTCTAGTGA TGTCATCAGT 
GAGGGACGAG TCAGAGTGGT TTTCTCTGTA CCGTGTGAAC CTCAGCTCGA TGCCTTAATA 
CAGAATCAGC ATAAATACTC TGTGAAGTAC AGTCCCGGGT ATGTACTCGC CTCACGCCTC 
GGTATCACTG GCTACCTCGT CTCAAGGTTC TCTGGTAGCA TCTTCATCGG AAGAGGATCC 
AAGAAAAATC CACATGGAGA GCAGAAAGTC AACGTAGGCC TTAATCTCAA GTTCAACAAA 
AAGAACGAGG AAGTTCCTGG CTACACCAAG AGAACAGAGA AGGAGTGGCT CTACTCTGCT 
GCTGTTGAGG AACTAATAGC TGAATACCTA GAACGATTTT CAGAGGTGTT TGACCATGTA 
GCACGAAACA GCCACGATGA CATATTTTAC GAAGACGACA TCTGGCCGGG AGAGGATGAG 
AATGGAGTGG AAAAGGTCCA AGAGATCCAG GGCTGGTTGA AAACGCATCC AGTGAGCTCC 
ATCTCCAGAG CATCATGTGA TCTGCAGATC CTGGATGCTG GTATTGTGGA GAAGATTGAG 
GAGGAGCTGG AGAAAACGAA GGCAAAGAAG ATGAACAAGA AAGTGCGGGT CACCGTGAAG 
CCCCATCTGC TTTTTAGGCC TCTGGAGCAG CAGCACGGTG TTGTTCCAGA TCCTGATTCA 
GAATATCATC TGTTTGACCG TGTGGTCAAT GTGAGAGAGA GCTTTTCTGT TCCACTCGGC 
CTTCGAGGAA CCATCATCGG CATCAAAGGA GCTGACCGTG AGGCTGAAAT TCTATATGAG 
GTGATATTTG ATGAGGAGTT TGCTGGAGGA CTCACTATCA GGTGTTCTCC TGGCCGTGGG 
TATCGTTTGC CCCCTAGTGC CTTAATCAAC CTGAGCCATG GCAGCAGGAA GGAGCTGGGC 
TCACACAAAC TCACTGCCAT CGTCAAACCT CAACCCTCAT CTTCATCACA AATCTCAAAC 
AAGAGCCAGC TGGGTGGCCT TAATCACTCA CCACGCTCAC CGTTCATACC TACACAGCAG 
CAGAATGGCA GACAAAGTTT TAATCTCAGG GGTGACACTC AGGGCAACAG GAACTCGCCA 
CATAAAGCCC TCAACCAGAG AAACCAACAA AAGGGGTCTA GTAATGAGAA AGAGTTCAGT 
AATGTATGGC AGTCCCTTCA GAATTCAGCA ATGCCCCAGA AACCACCAGC CCACTGGCAA 
AATAATGCTG GTAAAAGCCA GATGCCACAT CAGAATCAGC TGGAAGGACA AAATAAACAG 
AGCCAAGGCA ACGACACATC CATCAAACTT GGTTTGGGTG GTAATATTAG ACTCCTGAAG 
AGAAATGAGG ATCTAAATAC CGCTCTGCAG GGGCCTTCAA ATAAGGCATC TACAGAGTTT 
GAGGAGCTTA TAGCCAATCT TAAAATCTCA AAGGATCCTG AACCTCCTCA GCATGAACCA 
CCTAAAGAGC CCAAGGCCCC GTCAGCAGAG CCTCTCTCCC CACAATCCTT TGCCATGAAG 
GGAACTCTAG TATTGAAAGA GATGTTGAAG ATCGACAGCT CCGGAGCAAG TTCCTCTGCC 
CCTGATTCTG GCGGCCCCAA TGCCCAGCAG CAGTGCAAGA GACGCTCCAG CAAGAAACTA 
GCTGCTCGGA TAAACACTCC TCAGCCTGAA ACAGCTCCGG CTGCTGCTGC TGCTCCTCCT 
CCTCCTCCTC CTCCTCATCC TCTGGCTGGT CAGCCACCCC TCCTGCCCAG CATGGCCACT 
GAACTAGGAC GGGTGTGCAT GGGGCTCCGG ATGGGACCTC CAGAGTTTGC CTTCCTCCGC 
AACAGACAGA GTCTGATCTT GTGTCAGGTG AAGTTGTCGA ATGGTCTGAT GGTTCACGGC 
CCTCAGTGTC AGTCAGAAAC CGAAGCTAAA GAGAAAGCTG CTCTCTTCAC CCTTCAACGA 
TTGAACTCGC TGGGCTCTTT CCCATTGCCT CCTCCCATAT TCCCTGGAGT CCAGCAGATG 
AGACCGCTTG CACCTCATCC CTCCATGTTC GGACAGCCAG GAGGTGGGTT TCTGATGCCC 
CCTCAAGGGT ACGGGCCTCT TCCCTGGGGG ATGCAGTTTC CCCATCAGGG GCAGCCGTTC 
TTTGGAGGAA CTTTTCCTGG AGCACGAGGC CCTGTGCCAT CAGCAGCCGG CGGATCCCAT 
AACCAGTTTG TTCCCCTTCA GGTGACCAAG AAACGAGTTT CAGGCAGAAC CAAGAACCAA 
GAATCAAATC AAGCTCAGAG TGGCTCACCC TATGGCGTTC AGACCCCTGC AGTGCCTCCA 
TCAGCCCCTA AAACTCCACC ATCCACCAAC GCCCACGAGC ATGCAGAAAA GCCCACCGCA 
ACTACACCCA AGACCCCCAA GCAGAATGCC AATCCCCACA CCCCCGGCTC CGCATCCAAG 
AGGAAACACC GGAAACTGGC AGTCAACTTT GAGGCTGCTA AGGTGACGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

xrn1 ( XM_005163317.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163374.1 Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X1, mRNA.

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CloneID	ODa06835
Clone ID Related Accession (Same CDS sequence)	XM_005163317.4 , XM_005163317.3
Accession Version	XM_005163317.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	5'-3' exoribonuclease 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163317.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTGC CTAAGTTTTA TCGATGGATA TCAGAGCGGT ATCCGTGTCT GAGTGAAGTT 
GTCAAAGAGC ATCAGATCCC AGAGTTCGAC AACCTGTATT TAGACATGAA CGGGATCATC 
CACCAGTGTT CGCACCCAAA TGATGAGGAT GTGCACTTCC GTATTTCTGA AGAGAAGATA 
TTCGCTGACA TTTTTCACTA TCTGGAAGTG CTCTTTCGCA TCATTAAGCC AAGGAAAGTG 
TTCTTCATGG CCGTCGATGG AGTCGCACCC AGAGCCAAAA TGAACCAGCA GCGTGGGAGA 
AGATTCAGGT CAGCAAAAGA GGCAGAGGAG AAAATCAAGA AGGCCTTGGA GAAGGGAGAA 
GTTTTGCCCA CAGAGGCCCG ATTTGACTCT AACTGCATCA CTCCGGGAAC GGATTTCATG 
GCCAGGCTAC AGGAACAGCT TAAATATTTC GTTCACAATA AACTGTCCAC AGACAAAACA 
TGGCAGGGAG TGAATGTGTA TTTGTCTGGT CATGAGACCC CTGGTGAAGG AGAGCACAAA 
ATCATGGAGT TCATTCGCTC TGAGAGCACC AAACCGGGTC ATAACCCAAA CACAAGACAC 
TGCCTTTATG GCCTGGATGC CGATCTGATA ATGCTTGGCT TGACCAGCCA TGAGCCTCAT 
TTTTCTCTCC TCAGAGAGGA GGTTCGCTTT GGAGGCAAAA AGTCACAAAA GAGGATAACA 
GCTCCTGAGG AGACGACCTT TCATCTGCTT CATCTCTCCC TTATGAGGGA ATACATTGAC 
TATGAGTTTT CAGAGCTCAG GAAAAAGATC TCATTTGAGT ATGACCTGGA GCGGATCATA 
GACGACTGGG TTCTGATGGG TTTCCTGGTG GGGAATGACT TCATCCCTCA TCTTCCTCAC 
CTGCACATCA ATCATGACGC ACTCCCGCTG CTCTATCGCA CCTACATTAA TGTGCTGCCA 
AGTCTAGGAG GATACCTGAA TGAAAACGGA CACCTCAACC TCACCCACTT TGAGAAGTAC 
CTAGAGAAGT TATCAGATTT TGACCGGGAG CACTTCAGTG AGGTGTTTGT GGACCTGAAG 
TGGTTTGAGA GTAAGGTGGG GAACAAATAT GTGAACGAAG CTGCGGGGCT GGCAGCTGAG 
GAGGCTGATG GCAGAGACTT GAAGAAGAGT AAATCTTCAG ACGACTCTCT GTGTCTCGCG 
GCTCTTGATG GAGTCAGAGA TTCTCACAGC ACTGCAGGAA GAGAAGGCGC AGAGGAGGAC 
GGAGAGGACG ATGACATGTT TGAAACAGAA TTTCGACAAT ATAAACGCAC CTACTACATG 
ACCAAAATGG GTGTTGAAGT CGTCTCAGAT GAGTTTTTGG CCAATCAGGC TAAATGTTAC 
GTAGAGGGAA TCCAGTGGAT CTTGCATTAC TACTATCACG GAGTTCAGTC TTGGAGCTGG 
TATTATCCAT ACCACTACGC TCCTTTCCTC TCTGACGTGA GGAACATCTC TGGTCTCTCG 
CTGACCTTTG ACCTGGCCAA ACCCTTTATG CCGTTTGAGC AGCTCTTGGC CGTCCTGCCA 
TCAGCCAGCA AAGACCTTCT TCCTCAGTGC TACCAGCATC TGATGACGTC ACTGAGCTCC 
CCCATTGTCG AGTATTACCC ACAGGACTTC AAAACTGACC TGAACGGCAA ACAGCAGGAG 
TGGGAAGCAG TGGTGCTCAT CCCGTTCATT GACGAGAAAT GCTTGTTAGC AGCTATGGAG 
CCCTACACTC ATAATCTGAC CCCATCGGAG AAGGCCAGGA ACAGGCATAC GGAGTGTGCA 
GTTTACTGCT ACGATAAAGA CCTGGATTAT CCCTATAATT CCCCAGATCC GCAGATCTTC 
CCCAATATCG TCCACTGCCA TGCCAGGCAA ACACCGGTCC CGATGGATGC CTGGCGTGTA 
TCACCTGATC ATGTGCGATG CAGGATCGAC AGAGGAGCGC TTTACTTCTG CGGCTTCCCC 
ACATTACAGC ACATCCGTCA TAAATTTTAC AAGAAGAAAA GTGGAGTGAT GGTGTTTCAA 
CAAAGCAGCA GAGGAGAAAA CATGATCCTG GATATTTTGC CAAGCCATGA GATGGTGCAG 
GTGGAAGAGG TTGCAGCGCT GGTTCTTGGA AGATCTTTGT TTGTGAACTG GCCACATTTG 
ATGGAAGCTC GGGTTGTAGC CGTTTCAGAT GGAGAGACAA AATTTTCATT AGAGGAGCCT 
GCAGGATCAC AGGTGTTGTA CATGGAAAAC AATCCACCAC CCACTAAAGT CAGCCGTGTG 
ACTGATAAAG AGCAGAAGGA CTGGGTCAAA GAAGTGCAAG GCCTCACAGA GCATTTTAGC 
AAGCGTAAAG GCATAGCAAT CAATGAGACG GATGTGTTGG TTTACGGCCA GCTGCTGACA 
GGCAGGAAGT ATGTCATCGG TCAGAACGGA AAAGTCCATT TGGAGAAGCA GTTTGCCAAG 
CAGGTCCTGC CCTTTCCATA TCAGACCATT GTGAAGGATA TCAAGGCCTT TGATTCCTCA 
TTGGCGCGCT TCAAAACACT GGAGGAGCTG TTTCCTCTGG GAACCACAGT CTTTATGGTT 
GGAAATCCAT ATTACGGTGC AATGGGAGAA GTGCAAGACT CTAGTGATGT CATCAGTGAG 
GGACGAGTCA GAGTGGTTTT CTCTGTACCG TGTGAACCTC AGCTCGATGC CTTAATACAG 
AATCAGCATA AATACTCTGT GAAGTACAGT CCCGGGTATG TACTCGCCTC ACGCCTCGGT 
ATCACTGGCT ACCTCGTCTC AAGGTTCTCT GGTAGCATCT TCATCGGAAG AGGATCCAAG 
AAAAATCCAC ATGGAGAGCA GAAAGTCAAC GTAGGCCTTA ATCTCAAGTT CAACAAAAAG 
AACGAGGAAG TTCCTGGCTA CACCAAGAGA ACAGAGAAGG AGTGGCTCTA CTCTGCTGCT 
GTTGAGGAAC TAATAGCTGA ATACCTAGAA CGATTTTCAG AGGTGTTTGA CCATGTAGCA 
CGAAACAGCC ACGATGACAT ATTTTACGAA GACGACATCT GGCCGGGAGA GGATGAGAAT 
GGAGTGGAAA AGGTCCAAGA GATCCAGGGC TGGTTGAAAA CGCATCCAGT GAGCTCCATC 
TCCAGAGCAT CATGTGATCT GCAGATCCTG GATGCTGGTA TTGTGGAGAA GATTGAGGAG 
GAGCTGGAGA AAACGAAGGC AAAGAAGATG AACAAGAAAG TGCGGGTCAC CGTGAAGCCC 
CATCTGCTTT TTAGGCCTCT GGAGCAGCAG CACGGTGTTG TTCCAGATCC TGATTCAGAA 
TATCATCTGT TTGACCGTGT GGTCAATGTG AGAGAGAGCT TTTCTGTTCC ACTCGGCCTT 
CGAGGAACCA TCATCGGCAT CAAAGGAGCT GACCGTGAGG CTGAAATTCT ATATGAGGTG 
ATATTTGATG AGGAGTTTGC TGGAGGACTC ACTATCAGGT GTTCTCCTGG CCGTGGGTAT 
CGTTTGCCCC CTAGTGCCTT AATCAACCTG AGCCATGGCA GCAGGAAGGA GCTGGGCTCA 
CACAAACTCA CTGCCATCGT CAAACCTCAA CCCTCATCTT CATCACAAAT CTCAAACAAG 
AGCCAGCTGG GTGGCCTTAA TCACTCACCA CGCTCACCGT TCATACCTAC ACAGCAGCAG 
AATGGCAGAC AAAGTTTTAA TCTCAGGGGT GACACTCAGG GCAACAGGAA CTCGCCACAT 
AAAGCCCTCA ACCAGAGAAA CCAACAAAAG GGGTCTAGTA ATGAGAAAGA GTTCAGTAAT 
GTATGGCAGT CCCTTCAGAA TTCAGCAATG CCCCAGAAAC CACCAGCCCA CTGGCAAAAT 
AATGCTGGTA AAAGCCAGAT GCCACATCAG AATCAGCTGG AAGGACAAAA TAAACAGAGC 
CAAGGCAACG ACACATCCAT CAAACTTGGT TTGGGTGGTA ATATTAGACT CCTGAAGAGA 
AATGAGGATC TAAATACCGC TCTGCAGGGG CCTTCAAATA AGGCATCTAC AGAGTTTGAG 
GAGCTTATAG CCAATCTTAA AATCTCAAAG GATCCTGAAC CTCCTCAGCA TGAACCACCT 
AAAGAGCCCA AGGCCCCGTC AGCAGAGCCT CTCTCCCCAC AATCCTTTGC CATGAAGGGA 
ACTCTAGTAT TGAAAGAGAT GTTGAAGATC GACAGCTCCG GAGCAAGTTC CTCTGCCCCT 
GATTCTGGCG GCCCCAATGC CCAGCAGCAG TGCAAGAGAC GCTCCAGCAA GAAACTAGCT 
GCTCGGATAA ACACTCCTCA GCCTGAAACA GCTCCGGCTG CTGCTGCTGC TCCTCCTCCT 
CCTCCTCCTC CTCATCCTCT GGCTGGTCAG CCACCCCTCC TGCCCAGCAT GGCCACTGAA 
CTAGGACGGG TGTGCATGGG GCTCCGGATG GGACCTCCAG AGTTTGCCTT CCTCCGCAAC 
AGACAGAGTC TGATCTTGTG TCAGGTGAAG TTGTCGAATG GTCTGATGGT TCACGGCCCT 
CAGTGTCAGT CAGAAACCGA AGCTAAAGAG AAAGCTGCTC TCTTCACCCT TCAACGATTG 
AACTCGCTGG GCTCTTTCCC ATTGCCTCCT CCCATATTCC CTGGAGTCCA GCAGATGAGA 
CCGCTTGCAC CTCATCCCTC CATGTTCGGA CAGCCAGGAG GTGGGTTTCT GATGCCCCCT 
CAAGGGTACG GGCCTCTTCC CTGGGGGATG CAGTTTCCCC ATCAGGGGCA GCCGTTCTTT 
GGAGGAACTT TTCCTGGAGC ACGAGGCCCT GTGCCATCAG CAGCCGGCGG ATCCCATAAC 
CAGTTTGTTC CCCTTCAGGT GACCAAGAAA CGAGTTTCAG GCAGAACCAA GAACCAAGAA 
TCAAATCAAG CTCAGAGTGG CTCACCCTAT GGCGTTCAGA CCCCTGCAGT GCCTCCATCA 
GCCCCTAAAA CTCCACCATC CACCAACGCC CACGAGCATG CAGAAAAGCC CACCGCAACT 
ACACCCAAGA CCCCCAAGCA GAATGCCAAT CCCCACACCC CCGGCTCCGC ATCCAAGAGG 
AAACACCGGA AACTGGCAGT CAACTTTGAG GCTGCTAAGG TGACGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163374.1
CDS	309..5399
Translation	MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDEDVHFRISEEKIFADIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEEKIKKALEKGEVLPTEARFDSNCITPGTDFMARLQEQLKYFVHNKLSTDKTWQGVNVYLSGHETPGEGEHKIMEFIRSESTKPGHNPNTRHCLYGLDADLIMLGLTSHEPHFSLLREEVRFGGKKSQKRITAPEETTFHLLHLSLMREYIDYEFSELRKKISFEYDLERIIDDWVLMGFLVGNDFIPHLPHLHINHDALPLLYRTYINVLPSLGGYLNENGHLNLTHFEKYLEKLSDFDREHFSEVFVDLKWFESKVGNKYVNEAAGLAAEEADGRDLKKSKSSDDSLCLAALDGVRDSHSTAGREGAEEDGEDDDMFETEFRQYKRTYYMTKMGVEVVSDEFLANQAKCYVEGIQWILHYYYHGVQSWSWYYPYHYAPFLSDVRNISGLSLTFDLAKPFMPFEQLLAVLPSASKDLLPQCYQHLMTSLSSPIVEYYPQDFKTDLNGKQQEWEAVVLIPFIDEKCLLAAMEPYTHNLTPSEKARNRHTECAVYCYDKDLDYPYNSPDPQIFPNIVHCHARQTPVPMDAWRVSPDHVRCRIDRGALYFCGFPTLQHIRHKFYKKKSGVMVFQQSSRGENMILDILPSHEMVQVEEVAALVLGRSLFVNWPHLMEARVVAVSDGETKFSLEEPAGSQVLYMENNPPPTKVSRVTDKEQKDWVKEVQGLTEHFSKRKGIAINETDVLVYGQLLTGRKYVIGQNGKVHLEKQFAKQVLPFPYQTIVKDIKAFDSSLARFKTLEELFPLGTTVFMVGNPYYGAMGEVQDSSDVISEGRVRVVFSVPCEPQLDALIQNQHKYSVKYSPGYVLASRLGITGYLVSRFSGSIFIGRGSKKNPHGEQKVNVGLNLKFNKKNEEVPGYTKRTEKEWLYSAAVEELIAEYLERFSEVFDHVARNSHDDIFYEDDIWPGEDENGVEKVQEIQGWLKTHPVSSISRASCDLQILDAGIVEKIEEELEKTKAKKMNKKVRVTVKPHLLFRPLEQQHGVVPDPDSEYHLFDRVVNVRESFSVPLGLRGTIIGIKGADREAEILYEVIFDEEFAGGLTIRCSPGRGYRLPPSALINLSHGSRKELGSHKLTAIVKPQPSSSSQISNKSQLGGLNHSPRSPFIPTQQQNGRQSFNLRGDTQGNRNSPHKALNQRNQQKGSSNEKEFSNVWQSLQNSAMPQKPPAHWQNNAGKSQMPHQNQLEGQNKQSQGNDTSIKLGLGGNIRLLKRNEDLNTALQGPSNKASTEFEELIANLKISKDPEPPQHEPPKEPKAPSAEPLSPQSFAMKGTLVLKEMLKIDSSGASSSAPDSGGPNAQQQCKRRSSKKLAARINTPQPETAPAAAAAPPPPPPPHPLAGQPPLLPSMATELGRVCMGLRMGPPEFAFLRNRQSLILCQVKLSNGLMVHGPQCQSETEAKEKAALFTLQRLNSLGSFPLPPPIFPGVQQMRPLAPHPSMFGQPGGGFLMPPQGYGPLPWGMQFPHQGQPFFGGTFPGARGPVPSAAGGSHNQFVPLQVTKKRVSGRTKNQESNQAQSGSPYGVQTPAVPPSAPKTPPSTNAHEHAEKPTATTPKTPKQNANPHTPGSASKRKHRKLAVNFEAAKVTD

Target ORF information:

RefSeq Version	XM_005163317.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163317.4

ORF Insert Sequence:

ATGGGAGTGC CTAAGTTTTA TCGATGGATA TCAGAGCGGT ATCCGTGTCT GAGTGAAGTT 
GTCAAAGAGC ATCAGATCCC AGAGTTCGAC AACCTGTATT TAGACATGAA CGGGATCATC 
CACCAGTGTT CGCACCCAAA TGATGAGGAT GTGCACTTCC GTATTTCTGA AGAGAAGATA 
TTCGCTGACA TTTTTCACTA TCTGGAAGTG CTCTTTCGCA TCATTAAGCC AAGGAAAGTG 
TTCTTCATGG CCGTCGATGG AGTCGCACCC AGAGCCAAAA TGAACCAGCA GCGTGGGAGA 
AGATTCAGGT CAGCAAAAGA GGCAGAGGAG AAAATCAAGA AGGCCTTGGA GAAGGGAGAA 
GTTTTGCCCA CAGAGGCCCG ATTTGACTCT AACTGCATCA CTCCGGGAAC GGATTTCATG 
GCCAGGCTAC AGGAACAGCT TAAATATTTC GTTCACAATA AACTGTCCAC AGACAAAACA 
TGGCAGGGAG TGAATGTGTA TTTGTCTGGT CATGAGACCC CTGGTGAAGG AGAGCACAAA 
ATCATGGAGT TCATTCGCTC TGAGAGCACC AAACCGGGTC ATAACCCAAA CACAAGACAC 
TGCCTTTATG GCCTGGATGC CGATCTGATA ATGCTTGGCT TGACCAGCCA TGAGCCTCAT 
TTTTCTCTCC TCAGAGAGGA GGTTCGCTTT GGAGGCAAAA AGTCACAAAA GAGGATAACA 
GCTCCTGAGG AGACGACCTT TCATCTGCTT CATCTCTCCC TTATGAGGGA ATACATTGAC 
TATGAGTTTT CAGAGCTCAG GAAAAAGATC TCATTTGAGT ATGACCTGGA GCGGATCATA 
GACGACTGGG TTCTGATGGG TTTCCTGGTG GGGAATGACT TCATCCCTCA TCTTCCTCAC 
CTGCACATCA ATCATGACGC ACTCCCGCTG CTCTATCGCA CCTACATTAA TGTGCTGCCA 
AGTCTAGGAG GATACCTGAA TGAAAACGGA CACCTCAACC TCACCCACTT TGAGAAGTAC 
CTAGAGAAGT TATCAGATTT TGACCGGGAG CACTTCAGTG AGGTGTTTGT GGACCTGAAG 
TGGTTTGAGA GTAAGGTGGG GAACAAATAT GTGAACGAAG CTGCGGGGCT GGCAGCTGAG 
GAGGCTGATG GCAGAGACTT GAAGAAGAGT AAATCTTCAG ACGACTCTCT GTGTCTCGCG 
GCTCTTGATG GAGTCAGAGA TTCTCACAGC ACTGCAGGAA GAGAAGGCGC AGAGGAGGAC 
GGAGAGGACG ATGACATGTT TGAAACAGAA TTTCGACAAT ATAAACGCAC CTACTACATG 
ACCAAAATGG GTGTTGAAGT CGTCTCAGAT GAGTTTTTGG CCAATCAGGC TAAATGTTAC 
GTAGAGGGAA TCCAGTGGAT CTTGCATTAC TACTATCACG GAGTTCAGTC TTGGAGCTGG 
TATTATCCAT ACCACTACGC TCCTTTCCTC TCTGACGTGA GGAACATCTC TGGTCTCTCG 
CTGACCTTTG ACCTGGCCAA ACCCTTTATG CCGTTTGAGC AGCTCTTGGC CGTCCTGCCA 
TCAGCCAGCA AAGACCTTCT TCCTCAGTGC TACCAGCATC TGATGACGTC ACTGAGCTCC 
CCCATTGTCG AGTATTACCC ACAGGACTTC AAAACTGACC TGAACGGCAA ACAGCAGGAG 
TGGGAAGCAG TGGTGCTCAT CCCGTTCATT GACGAGAAAT GCTTGTTAGC AGCTATGGAG 
CCCTACACTC ATAATCTGAC CCCATCGGAG AAGGCCAGGA ACAGGCATAC GGAGTGTGCA 
GTTTACTGCT ACGATAAAGA CCTGGATTAT CCCTATAATT CCCCAGATCC GCAGATCTTC 
CCCAATATCG TCCACTGCCA TGCCAGGCAA ACACCGGTCC CGATGGATGC CTGGCGTGTA 
TCACCTGATC ATGTGCGATG CAGGATCGAC AGAGGAGCGC TTTACTTCTG CGGCTTCCCC 
ACATTACAGC ACATCCGTCA TAAATTTTAC AAGAAGAAAA GTGGAGTGAT GGTGTTTCAA 
CAAAGCAGCA GAGGAGAAAA CATGATCCTG GATATTTTGC CAAGCCATGA GATGGTGCAG 
GTGGAAGAGG TTGCAGCGCT GGTTCTTGGA AGATCTTTGT TTGTGAACTG GCCACATTTG 
ATGGAAGCTC GGGTTGTAGC CGTTTCAGAT GGAGAGACAA AATTTTCATT AGAGGAGCCT 
GCAGGATCAC AGGTGTTGTA CATGGAAAAC AATCCACCAC CCACTAAAGT CAGCCGTGTG 
ACTGATAAAG AGCAGAAGGA CTGGGTCAAA GAAGTGCAAG GCCTCACAGA GCATTTTAGC 
AAGCGTAAAG GCATAGCAAT CAATGAGACG GATGTGTTGG TTTACGGCCA GCTGCTGACA 
GGCAGGAAGT ATGTCATCGG TCAGAACGGA AAAGTCCATT TGGAGAAGCA GTTTGCCAAG 
CAGGTCCTGC CCTTTCCATA TCAGACCATT GTGAAGGATA TCAAGGCCTT TGATTCCTCA 
TTGGCGCGCT TCAAAACACT GGAGGAGCTG TTTCCTCTGG GAACCACAGT CTTTATGGTT 
GGAAATCCAT ATTACGGTGC AATGGGAGAA GTGCAAGACT CTAGTGATGT CATCAGTGAG 
GGACGAGTCA GAGTGGTTTT CTCTGTACCG TGTGAACCTC AGCTCGATGC CTTAATACAG 
AATCAGCATA AATACTCTGT GAAGTACAGT CCCGGGTATG TACTCGCCTC ACGCCTCGGT 
ATCACTGGCT ACCTCGTCTC AAGGTTCTCT GGTAGCATCT TCATCGGAAG AGGATCCAAG 
AAAAATCCAC ATGGAGAGCA GAAAGTCAAC GTAGGCCTTA ATCTCAAGTT CAACAAAAAG 
AACGAGGAAG TTCCTGGCTA CACCAAGAGA ACAGAGAAGG AGTGGCTCTA CTCTGCTGCT 
GTTGAGGAAC TAATAGCTGA ATACCTAGAA CGATTTTCAG AGGTGTTTGA CCATGTAGCA 
CGAAACAGCC ACGATGACAT ATTTTACGAA GACGACATCT GGCCGGGAGA GGATGAGAAT 
GGAGTGGAAA AGGTCCAAGA GATCCAGGGC TGGTTGAAAA CGCATCCAGT GAGCTCCATC 
TCCAGAGCAT CATGTGATCT GCAGATCCTG GATGCTGGTA TTGTGGAGAA GATTGAGGAG 
GAGCTGGAGA AAACGAAGGC AAAGAAGATG AACAAGAAAG TGCGGGTCAC CGTGAAGCCC 
CATCTGCTTT TTAGGCCTCT GGAGCAGCAG CACGGTGTTG TTCCAGATCC TGATTCAGAA 
TATCATCTGT TTGACCGTGT GGTCAATGTG AGAGAGAGCT TTTCTGTTCC ACTCGGCCTT 
CGAGGAACCA TCATCGGCAT CAAAGGAGCT GACCGTGAGG CTGAAATTCT ATATGAGGTG 
ATATTTGATG AGGAGTTTGC TGGAGGACTC ACTATCAGGT GTTCTCCTGG CCGTGGGTAT 
CGTTTGCCCC CTAGTGCCTT AATCAACCTG AGCCATGGCA GCAGGAAGGA GCTGGGCTCA 
CACAAACTCA CTGCCATCGT CAAACCTCAA CCCTCATCTT CATCACAAAT CTCAAACAAG 
AGCCAGCTGG GTGGCCTTAA TCACTCACCA CGCTCACCGT TCATACCTAC ACAGCAGCAG 
AATGGCAGAC AAAGTTTTAA TCTCAGGGGT GACACTCAGG GCAACAGGAA CTCGCCACAT 
AAAGCCCTCA ACCAGAGAAA CCAACAAAAG GGGTCTAGTA ATGAGAAAGA GTTCAGTAAT 
GTATGGCAGT CCCTTCAGAA TTCAGCAATG CCCCAGAAAC CACCAGCCCA CTGGCAAAAT 
AATGCTGGTA AAAGCCAGAT GCCACATCAG AATCAGCTGG AAGGACAAAA TAAACAGAGC 
CAAGGCAACG ACACATCCAT CAAACTTGGT TTGGGTGGTA ATATTAGACT CCTGAAGAGA 
AATGAGGATC TAAATACCGC TCTGCAGGGG CCTTCAAATA AGGCATCTAC AGAGTTTGAG 
GAGCTTATAG CCAATCTTAA AATCTCAAAG GATCCTGAAC CTCCTCAGCA TGAACCACCT 
AAAGAGCCCA AGGCCCCGTC AGCAGAGCCT CTCTCCCCAC AATCCTTTGC CATGAAGGGA 
ACTCTAGTAT TGAAAGAGAT GTTGAAGATC GACAGCTCCG GAGCAAGTTC CTCTGCCCCT 
GATTCTGGCG GCCCCAATGC CCAGCAGCAG TGCAAGAGAC GCTCCAGCAA GAAACTAGCT 
GCTCGGATAA ACACTCCTCA GCCTGAAACA GCTCCGGCTG CTGCTGCTGC TCCTCCTCCT 
CCTCCTCCTC CTCATCCTCT GGCTGGTCAG CCACCCCTCC TGCCCAGCAT GGCCACTGAA 
CTAGGACGGG TGTGCATGGG GCTCCGGATG GGACCTCCAG AGTTTGCCTT CCTCCGCAAC 
AGACAGAGTC TGATCTTGTG TCAGGTGAAG TTGTCGAATG GTCTGATGGT TCACGGCCCT 
CAGTGTCAGT CAGAAACCGA AGCTAAAGAG AAAGCTGCTC TCTTCACCCT TCAACGATTG 
AACTCGCTGG GCTCTTTCCC ATTGCCTCCT CCCATATTCC CTGGAGTCCA GCAGATGAGA 
CCGCTTGCAC CTCATCCCTC CATGTTCGGA CAGCCAGGAG GTGGGTTTCT GATGCCCCCT 
CAAGGGTACG GGCCTCTTCC CTGGGGGATG CAGTTTCCCC ATCAGGGGCA GCCGTTCTTT 
GGAGGAACTT TTCCTGGAGC ACGAGGCCCT GTGCCATCAG CAGCCGGCGG ATCCCATAAC 
CAGTTTGTTC CCCTTCAGGT GACCAAGAAA CGAGTTTCAG GCAGAACCAA GAACCAAGAA 
TCAAATCAAG CTCAGAGTGG CTCACCCTAT GGCGTTCAGA CCCCTGCAGT GCCTCCATCA 
GCCCCTAAAA CTCCACCATC CACCAACGCC CACGAGCATG CAGAAAAGCC CACCGCAACT 
ACACCCAAGA CCCCCAAGCA GAATGCCAAT CCCCACACCC CCGGCTCCGC ATCCAAGAGG 
AAACACCGGA AACTGGCAGT CAACTTTGAG GCTGCTAAGG TGACGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

xrn1 ( XM_005163317.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163374.1 Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06835
Clone ID Related Accession (Same CDS sequence)	XM_005163317.4 , XM_005163317.3
Accession Version	XM_005163317.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	5'-3' exoribonuclease 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163317.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTGC CTAAGTTTTA TCGATGGATA TCAGAGCGGT ATCCGTGTCT GAGTGAAGTT 
GTCAAAGAGC ATCAGATCCC AGAGTTCGAC AACCTGTATT TAGACATGAA CGGGATCATC 
CACCAGTGTT CGCACCCAAA TGATGAGGAT GTGCACTTCC GTATTTCTGA AGAGAAGATA 
TTCGCTGACA TTTTTCACTA TCTGGAAGTG CTCTTTCGCA TCATTAAGCC AAGGAAAGTG 
TTCTTCATGG CCGTCGATGG AGTCGCACCC AGAGCCAAAA TGAACCAGCA GCGTGGGAGA 
AGATTCAGGT CAGCAAAAGA GGCAGAGGAG AAAATCAAGA AGGCCTTGGA GAAGGGAGAA 
GTTTTGCCCA CAGAGGCCCG ATTTGACTCT AACTGCATCA CTCCGGGAAC GGATTTCATG 
GCCAGGCTAC AGGAACAGCT TAAATATTTC GTTCACAATA AACTGTCCAC AGACAAAACA 
TGGCAGGGAG TGAATGTGTA TTTGTCTGGT CATGAGACCC CTGGTGAAGG AGAGCACAAA 
ATCATGGAGT TCATTCGCTC TGAGAGCACC AAACCGGGTC ATAACCCAAA CACAAGACAC 
TGCCTTTATG GCCTGGATGC CGATCTGATA ATGCTTGGCT TGACCAGCCA TGAGCCTCAT 
TTTTCTCTCC TCAGAGAGGA GGTTCGCTTT GGAGGCAAAA AGTCACAAAA GAGGATAACA 
GCTCCTGAGG AGACGACCTT TCATCTGCTT CATCTCTCCC TTATGAGGGA ATACATTGAC 
TATGAGTTTT CAGAGCTCAG GAAAAAGATC TCATTTGAGT ATGACCTGGA GCGGATCATA 
GACGACTGGG TTCTGATGGG TTTCCTGGTG GGGAATGACT TCATCCCTCA TCTTCCTCAC 
CTGCACATCA ATCATGACGC ACTCCCGCTG CTCTATCGCA CCTACATTAA TGTGCTGCCA 
AGTCTAGGAG GATACCTGAA TGAAAACGGA CACCTCAACC TCACCCACTT TGAGAAGTAC 
CTAGAGAAGT TATCAGATTT TGACCGGGAG CACTTCAGTG AGGTGTTTGT GGACCTGAAG 
TGGTTTGAGA GTAAGGTGGG GAACAAATAT GTGAACGAAG CTGCGGGGCT GGCAGCTGAG 
GAGGCTGATG GCAGAGACTT GAAGAAGAGT AAATCTTCAG ACGACTCTCT GTGTCTCGCG 
GCTCTTGATG GAGTCAGAGA TTCTCACAGC ACTGCAGGAA GAGAAGGCGC AGAGGAGGAC 
GGAGAGGACG ATGACATGTT TGAAACAGAA TTTCGACAAT ATAAACGCAC CTACTACATG 
ACCAAAATGG GTGTTGAAGT CGTCTCAGAT GAGTTTTTGG CCAATCAGGC TAAATGTTAC 
GTAGAGGGAA TCCAGTGGAT CTTGCATTAC TACTATCACG GAGTTCAGTC TTGGAGCTGG 
TATTATCCAT ACCACTACGC TCCTTTCCTC TCTGACGTGA GGAACATCTC TGGTCTCTCG 
CTGACCTTTG ACCTGGCCAA ACCCTTTATG CCGTTTGAGC AGCTCTTGGC CGTCCTGCCA 
TCAGCCAGCA AAGACCTTCT TCCTCAGTGC TACCAGCATC TGATGACGTC ACTGAGCTCC 
CCCATTGTCG AGTATTACCC ACAGGACTTC AAAACTGACC TGAACGGCAA ACAGCAGGAG 
TGGGAAGCAG TGGTGCTCAT CCCGTTCATT GACGAGAAAT GCTTGTTAGC AGCTATGGAG 
CCCTACACTC ATAATCTGAC CCCATCGGAG AAGGCCAGGA ACAGGCATAC GGAGTGTGCA 
GTTTACTGCT ACGATAAAGA CCTGGATTAT CCCTATAATT CCCCAGATCC GCAGATCTTC 
CCCAATATCG TCCACTGCCA TGCCAGGCAA ACACCGGTCC CGATGGATGC CTGGCGTGTA 
TCACCTGATC ATGTGCGATG CAGGATCGAC AGAGGAGCGC TTTACTTCTG CGGCTTCCCC 
ACATTACAGC ACATCCGTCA TAAATTTTAC AAGAAGAAAA GTGGAGTGAT GGTGTTTCAA 
CAAAGCAGCA GAGGAGAAAA CATGATCCTG GATATTTTGC CAAGCCATGA GATGGTGCAG 
GTGGAAGAGG TTGCAGCGCT GGTTCTTGGA AGATCTTTGT TTGTGAACTG GCCACATTTG 
ATGGAAGCTC GGGTTGTAGC CGTTTCAGAT GGAGAGACAA AATTTTCATT AGAGGAGCCT 
GCAGGATCAC AGGTGTTGTA CATGGAAAAC AATCCACCAC CCACTAAAGT CAGCCGTGTG 
ACTGATAAAG AGCAGAAGGA CTGGGTCAAA GAAGTGCAAG GCCTCACAGA GCATTTTAGC 
AAGCGTAAAG GCATAGCAAT CAATGAGACG GATGTGTTGG TTTACGGCCA GCTGCTGACA 
GGCAGGAAGT ATGTCATCGG TCAGAACGGA AAAGTCCATT TGGAGAAGCA GTTTGCCAAG 
CAGGTCCTGC CCTTTCCATA TCAGACCATT GTGAAGGATA TCAAGGCCTT TGATTCCTCA 
TTGGCGCGCT TCAAAACACT GGAGGAGCTG TTTCCTCTGG GAACCACAGT CTTTATGGTT 
GGAAATCCAT ATTACGGTGC AATGGGAGAA GTGCAAGACT CTAGTGATGT CATCAGTGAG 
GGACGAGTCA GAGTGGTTTT CTCTGTACCG TGTGAACCTC AGCTCGATGC CTTAATACAG 
AATCAGCATA AATACTCTGT GAAGTACAGT CCCGGGTATG TACTCGCCTC ACGCCTCGGT 
ATCACTGGCT ACCTCGTCTC AAGGTTCTCT GGTAGCATCT TCATCGGAAG AGGATCCAAG 
AAAAATCCAC ATGGAGAGCA GAAAGTCAAC GTAGGCCTTA ATCTCAAGTT CAACAAAAAG 
AACGAGGAAG TTCCTGGCTA CACCAAGAGA ACAGAGAAGG AGTGGCTCTA CTCTGCTGCT 
GTTGAGGAAC TAATAGCTGA ATACCTAGAA CGATTTTCAG AGGTGTTTGA CCATGTAGCA 
CGAAACAGCC ACGATGACAT ATTTTACGAA GACGACATCT GGCCGGGAGA GGATGAGAAT 
GGAGTGGAAA AGGTCCAAGA GATCCAGGGC TGGTTGAAAA CGCATCCAGT GAGCTCCATC 
TCCAGAGCAT CATGTGATCT GCAGATCCTG GATGCTGGTA TTGTGGAGAA GATTGAGGAG 
GAGCTGGAGA AAACGAAGGC AAAGAAGATG AACAAGAAAG TGCGGGTCAC CGTGAAGCCC 
CATCTGCTTT TTAGGCCTCT GGAGCAGCAG CACGGTGTTG TTCCAGATCC TGATTCAGAA 
TATCATCTGT TTGACCGTGT GGTCAATGTG AGAGAGAGCT TTTCTGTTCC ACTCGGCCTT 
CGAGGAACCA TCATCGGCAT CAAAGGAGCT GACCGTGAGG CTGAAATTCT ATATGAGGTG 
ATATTTGATG AGGAGTTTGC TGGAGGACTC ACTATCAGGT GTTCTCCTGG CCGTGGGTAT 
CGTTTGCCCC CTAGTGCCTT AATCAACCTG AGCCATGGCA GCAGGAAGGA GCTGGGCTCA 
CACAAACTCA CTGCCATCGT CAAACCTCAA CCCTCATCTT CATCACAAAT CTCAAACAAG 
AGCCAGCTGG GTGGCCTTAA TCACTCACCA CGCTCACCGT TCATACCTAC ACAGCAGCAG 
AATGGCAGAC AAAGTTTTAA TCTCAGGGGT GACACTCAGG GCAACAGGAA CTCGCCACAT 
AAAGCCCTCA ACCAGAGAAA CCAACAAAAG GGGTCTAGTA ATGAGAAAGA GTTCAGTAAT 
GTATGGCAGT CCCTTCAGAA TTCAGCAATG CCCCAGAAAC CACCAGCCCA CTGGCAAAAT 
AATGCTGGTA AAAGCCAGAT GCCACATCAG AATCAGCTGG AAGGACAAAA TAAACAGAGC 
CAAGGCAACG ACACATCCAT CAAACTTGGT TTGGGTGGTA ATATTAGACT CCTGAAGAGA 
AATGAGGATC TAAATACCGC TCTGCAGGGG CCTTCAAATA AGGCATCTAC AGAGTTTGAG 
GAGCTTATAG CCAATCTTAA AATCTCAAAG GATCCTGAAC CTCCTCAGCA TGAACCACCT 
AAAGAGCCCA AGGCCCCGTC AGCAGAGCCT CTCTCCCCAC AATCCTTTGC CATGAAGGGA 
ACTCTAGTAT TGAAAGAGAT GTTGAAGATC GACAGCTCCG GAGCAAGTTC CTCTGCCCCT 
GATTCTGGCG GCCCCAATGC CCAGCAGCAG TGCAAGAGAC GCTCCAGCAA GAAACTAGCT 
GCTCGGATAA ACACTCCTCA GCCTGAAACA GCTCCGGCTG CTGCTGCTGC TCCTCCTCCT 
CCTCCTCCTC CTCATCCTCT GGCTGGTCAG CCACCCCTCC TGCCCAGCAT GGCCACTGAA 
CTAGGACGGG TGTGCATGGG GCTCCGGATG GGACCTCCAG AGTTTGCCTT CCTCCGCAAC 
AGACAGAGTC TGATCTTGTG TCAGGTGAAG TTGTCGAATG GTCTGATGGT TCACGGCCCT 
CAGTGTCAGT CAGAAACCGA AGCTAAAGAG AAAGCTGCTC TCTTCACCCT TCAACGATTG 
AACTCGCTGG GCTCTTTCCC ATTGCCTCCT CCCATATTCC CTGGAGTCCA GCAGATGAGA 
CCGCTTGCAC CTCATCCCTC CATGTTCGGA CAGCCAGGAG GTGGGTTTCT GATGCCCCCT 
CAAGGGTACG GGCCTCTTCC CTGGGGGATG CAGTTTCCCC ATCAGGGGCA GCCGTTCTTT 
GGAGGAACTT TTCCTGGAGC ACGAGGCCCT GTGCCATCAG CAGCCGGCGG ATCCCATAAC 
CAGTTTGTTC CCCTTCAGGT GACCAAGAAA CGAGTTTCAG GCAGAACCAA GAACCAAGAA 
TCAAATCAAG CTCAGAGTGG CTCACCCTAT GGCGTTCAGA CCCCTGCAGT GCCTCCATCA 
GCCCCTAAAA CTCCACCATC CACCAACGCC CACGAGCATG CAGAAAAGCC CACCGCAACT 
ACACCCAAGA CCCCCAAGCA GAATGCCAAT CCCCACACCC CCGGCTCCGC ATCCAAGAGG 
AAACACCGGA AACTGGCAGT CAACTTTGAG GCTGCTAAGG TGACGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163374.1
CDS	234..5324
Translation	MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDEDVHFRISEEKIFADIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEEKIKKALEKGEVLPTEARFDSNCITPGTDFMARLQEQLKYFVHNKLSTDKTWQGVNVYLSGHETPGEGEHKIMEFIRSESTKPGHNPNTRHCLYGLDADLIMLGLTSHEPHFSLLREEVRFGGKKSQKRITAPEETTFHLLHLSLMREYIDYEFSELRKKISFEYDLERIIDDWVLMGFLVGNDFIPHLPHLHINHDALPLLYRTYINVLPSLGGYLNENGHLNLTHFEKYLEKLSDFDREHFSEVFVDLKWFESKVGNKYVNEAAGLAAEEADGRDLKKSKSSDDSLCLAALDGVRDSHSTAGREGAEEDGEDDDMFETEFRQYKRTYYMTKMGVEVVSDEFLANQAKCYVEGIQWILHYYYHGVQSWSWYYPYHYAPFLSDVRNISGLSLTFDLAKPFMPFEQLLAVLPSASKDLLPQCYQHLMTSLSSPIVEYYPQDFKTDLNGKQQEWEAVVLIPFIDEKCLLAAMEPYTHNLTPSEKARNRHTECAVYCYDKDLDYPYNSPDPQIFPNIVHCHARQTPVPMDAWRVSPDHVRCRIDRGALYFCGFPTLQHIRHKFYKKKSGVMVFQQSSRGENMILDILPSHEMVQVEEVAALVLGRSLFVNWPHLMEARVVAVSDGETKFSLEEPAGSQVLYMENNPPPTKVSRVTDKEQKDWVKEVQGLTEHFSKRKGIAINETDVLVYGQLLTGRKYVIGQNGKVHLEKQFAKQVLPFPYQTIVKDIKAFDSSLARFKTLEELFPLGTTVFMVGNPYYGAMGEVQDSSDVISEGRVRVVFSVPCEPQLDALIQNQHKYSVKYSPGYVLASRLGITGYLVSRFSGSIFIGRGSKKNPHGEQKVNVGLNLKFNKKNEEVPGYTKRTEKEWLYSAAVEELIAEYLERFSEVFDHVARNSHDDIFYEDDIWPGEDENGVEKVQEIQGWLKTHPVSSISRASCDLQILDAGIVEKIEEELEKTKAKKMNKKVRVTVKPHLLFRPLEQQHGVVPDPDSEYHLFDRVVNVRESFSVPLGLRGTIIGIKGADREAEILYEVIFDEEFAGGLTIRCSPGRGYRLPPSALINLSHGSRKELGSHKLTAIVKPQPSSSSQISNKSQLGGLNHSPRSPFIPTQQQNGRQSFNLRGDTQGNRNSPHKALNQRNQQKGSSNEKEFSNVWQSLQNSAMPQKPPAHWQNNAGKSQMPHQNQLEGQNKQSQGNDTSIKLGLGGNIRLLKRNEDLNTALQGPSNKASTEFEELIANLKISKDPEPPQHEPPKEPKAPSAEPLSPQSFAMKGTLVLKEMLKIDSSGASSSAPDSGGPNAQQQCKRRSSKKLAARINTPQPETAPAAAAAPPPPPPPHPLAGQPPLLPSMATELGRVCMGLRMGPPEFAFLRNRQSLILCQVKLSNGLMVHGPQCQSETEAKEKAALFTLQRLNSLGSFPLPPPIFPGVQQMRPLAPHPSMFGQPGGGFLMPPQGYGPLPWGMQFPHQGQPFFGGTFPGARGPVPSAAGGSHNQFVPLQVTKKRVSGRTKNQESNQAQSGSPYGVQTPAVPPSAPKTPPSTNAHEHAEKPTATTPKTPKQNANPHTPGSASKRKHRKLAVNFEAAKVTD

Target ORF information:

RefSeq Version	XM_005163317.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163317.3

ORF Insert Sequence:

ATGGGAGTGC CTAAGTTTTA TCGATGGATA TCAGAGCGGT ATCCGTGTCT GAGTGAAGTT 
GTCAAAGAGC ATCAGATCCC AGAGTTCGAC AACCTGTATT TAGACATGAA CGGGATCATC 
CACCAGTGTT CGCACCCAAA TGATGAGGAT GTGCACTTCC GTATTTCTGA AGAGAAGATA 
TTCGCTGACA TTTTTCACTA TCTGGAAGTG CTCTTTCGCA TCATTAAGCC AAGGAAAGTG 
TTCTTCATGG CCGTCGATGG AGTCGCACCC AGAGCCAAAA TGAACCAGCA GCGTGGGAGA 
AGATTCAGGT CAGCAAAAGA GGCAGAGGAG AAAATCAAGA AGGCCTTGGA GAAGGGAGAA 
GTTTTGCCCA CAGAGGCCCG ATTTGACTCT AACTGCATCA CTCCGGGAAC GGATTTCATG 
GCCAGGCTAC AGGAACAGCT TAAATATTTC GTTCACAATA AACTGTCCAC AGACAAAACA 
TGGCAGGGAG TGAATGTGTA TTTGTCTGGT CATGAGACCC CTGGTGAAGG AGAGCACAAA 
ATCATGGAGT TCATTCGCTC TGAGAGCACC AAACCGGGTC ATAACCCAAA CACAAGACAC 
TGCCTTTATG GCCTGGATGC CGATCTGATA ATGCTTGGCT TGACCAGCCA TGAGCCTCAT 
TTTTCTCTCC TCAGAGAGGA GGTTCGCTTT GGAGGCAAAA AGTCACAAAA GAGGATAACA 
GCTCCTGAGG AGACGACCTT TCATCTGCTT CATCTCTCCC TTATGAGGGA ATACATTGAC 
TATGAGTTTT CAGAGCTCAG GAAAAAGATC TCATTTGAGT ATGACCTGGA GCGGATCATA 
GACGACTGGG TTCTGATGGG TTTCCTGGTG GGGAATGACT TCATCCCTCA TCTTCCTCAC 
CTGCACATCA ATCATGACGC ACTCCCGCTG CTCTATCGCA CCTACATTAA TGTGCTGCCA 
AGTCTAGGAG GATACCTGAA TGAAAACGGA CACCTCAACC TCACCCACTT TGAGAAGTAC 
CTAGAGAAGT TATCAGATTT TGACCGGGAG CACTTCAGTG AGGTGTTTGT GGACCTGAAG 
TGGTTTGAGA GTAAGGTGGG GAACAAATAT GTGAACGAAG CTGCGGGGCT GGCAGCTGAG 
GAGGCTGATG GCAGAGACTT GAAGAAGAGT AAATCTTCAG ACGACTCTCT GTGTCTCGCG 
GCTCTTGATG GAGTCAGAGA TTCTCACAGC ACTGCAGGAA GAGAAGGCGC AGAGGAGGAC 
GGAGAGGACG ATGACATGTT TGAAACAGAA TTTCGACAAT ATAAACGCAC CTACTACATG 
ACCAAAATGG GTGTTGAAGT CGTCTCAGAT GAGTTTTTGG CCAATCAGGC TAAATGTTAC 
GTAGAGGGAA TCCAGTGGAT CTTGCATTAC TACTATCACG GAGTTCAGTC TTGGAGCTGG 
TATTATCCAT ACCACTACGC TCCTTTCCTC TCTGACGTGA GGAACATCTC TGGTCTCTCG 
CTGACCTTTG ACCTGGCCAA ACCCTTTATG CCGTTTGAGC AGCTCTTGGC CGTCCTGCCA 
TCAGCCAGCA AAGACCTTCT TCCTCAGTGC TACCAGCATC TGATGACGTC ACTGAGCTCC 
CCCATTGTCG AGTATTACCC ACAGGACTTC AAAACTGACC TGAACGGCAA ACAGCAGGAG 
TGGGAAGCAG TGGTGCTCAT CCCGTTCATT GACGAGAAAT GCTTGTTAGC AGCTATGGAG 
CCCTACACTC ATAATCTGAC CCCATCGGAG AAGGCCAGGA ACAGGCATAC GGAGTGTGCA 
GTTTACTGCT ACGATAAAGA CCTGGATTAT CCCTATAATT CCCCAGATCC GCAGATCTTC 
CCCAATATCG TCCACTGCCA TGCCAGGCAA ACACCGGTCC CGATGGATGC CTGGCGTGTA 
TCACCTGATC ATGTGCGATG CAGGATCGAC AGAGGAGCGC TTTACTTCTG CGGCTTCCCC 
ACATTACAGC ACATCCGTCA TAAATTTTAC AAGAAGAAAA GTGGAGTGAT GGTGTTTCAA 
CAAAGCAGCA GAGGAGAAAA CATGATCCTG GATATTTTGC CAAGCCATGA GATGGTGCAG 
GTGGAAGAGG TTGCAGCGCT GGTTCTTGGA AGATCTTTGT TTGTGAACTG GCCACATTTG 
ATGGAAGCTC GGGTTGTAGC CGTTTCAGAT GGAGAGACAA AATTTTCATT AGAGGAGCCT 
GCAGGATCAC AGGTGTTGTA CATGGAAAAC AATCCACCAC CCACTAAAGT CAGCCGTGTG 
ACTGATAAAG AGCAGAAGGA CTGGGTCAAA GAAGTGCAAG GCCTCACAGA GCATTTTAGC 
AAGCGTAAAG GCATAGCAAT CAATGAGACG GATGTGTTGG TTTACGGCCA GCTGCTGACA 
GGCAGGAAGT ATGTCATCGG TCAGAACGGA AAAGTCCATT TGGAGAAGCA GTTTGCCAAG 
CAGGTCCTGC CCTTTCCATA TCAGACCATT GTGAAGGATA TCAAGGCCTT TGATTCCTCA 
TTGGCGCGCT TCAAAACACT GGAGGAGCTG TTTCCTCTGG GAACCACAGT CTTTATGGTT 
GGAAATCCAT ATTACGGTGC AATGGGAGAA GTGCAAGACT CTAGTGATGT CATCAGTGAG 
GGACGAGTCA GAGTGGTTTT CTCTGTACCG TGTGAACCTC AGCTCGATGC CTTAATACAG 
AATCAGCATA AATACTCTGT GAAGTACAGT CCCGGGTATG TACTCGCCTC ACGCCTCGGT 
ATCACTGGCT ACCTCGTCTC AAGGTTCTCT GGTAGCATCT TCATCGGAAG AGGATCCAAG 
AAAAATCCAC ATGGAGAGCA GAAAGTCAAC GTAGGCCTTA ATCTCAAGTT CAACAAAAAG 
AACGAGGAAG TTCCTGGCTA CACCAAGAGA ACAGAGAAGG AGTGGCTCTA CTCTGCTGCT 
GTTGAGGAAC TAATAGCTGA ATACCTAGAA CGATTTTCAG AGGTGTTTGA CCATGTAGCA 
CGAAACAGCC ACGATGACAT ATTTTACGAA GACGACATCT GGCCGGGAGA GGATGAGAAT 
GGAGTGGAAA AGGTCCAAGA GATCCAGGGC TGGTTGAAAA CGCATCCAGT GAGCTCCATC 
TCCAGAGCAT CATGTGATCT GCAGATCCTG GATGCTGGTA TTGTGGAGAA GATTGAGGAG 
GAGCTGGAGA AAACGAAGGC AAAGAAGATG AACAAGAAAG TGCGGGTCAC CGTGAAGCCC 
CATCTGCTTT TTAGGCCTCT GGAGCAGCAG CACGGTGTTG TTCCAGATCC TGATTCAGAA 
TATCATCTGT TTGACCGTGT GGTCAATGTG AGAGAGAGCT TTTCTGTTCC ACTCGGCCTT 
CGAGGAACCA TCATCGGCAT CAAAGGAGCT GACCGTGAGG CTGAAATTCT ATATGAGGTG 
ATATTTGATG AGGAGTTTGC TGGAGGACTC ACTATCAGGT GTTCTCCTGG CCGTGGGTAT 
CGTTTGCCCC CTAGTGCCTT AATCAACCTG AGCCATGGCA GCAGGAAGGA GCTGGGCTCA 
CACAAACTCA CTGCCATCGT CAAACCTCAA CCCTCATCTT CATCACAAAT CTCAAACAAG 
AGCCAGCTGG GTGGCCTTAA TCACTCACCA CGCTCACCGT TCATACCTAC ACAGCAGCAG 
AATGGCAGAC AAAGTTTTAA TCTCAGGGGT GACACTCAGG GCAACAGGAA CTCGCCACAT 
AAAGCCCTCA ACCAGAGAAA CCAACAAAAG GGGTCTAGTA ATGAGAAAGA GTTCAGTAAT 
GTATGGCAGT CCCTTCAGAA TTCAGCAATG CCCCAGAAAC CACCAGCCCA CTGGCAAAAT 
AATGCTGGTA AAAGCCAGAT GCCACATCAG AATCAGCTGG AAGGACAAAA TAAACAGAGC 
CAAGGCAACG ACACATCCAT CAAACTTGGT TTGGGTGGTA ATATTAGACT CCTGAAGAGA 
AATGAGGATC TAAATACCGC TCTGCAGGGG CCTTCAAATA AGGCATCTAC AGAGTTTGAG 
GAGCTTATAG CCAATCTTAA AATCTCAAAG GATCCTGAAC CTCCTCAGCA TGAACCACCT 
AAAGAGCCCA AGGCCCCGTC AGCAGAGCCT CTCTCCCCAC AATCCTTTGC CATGAAGGGA 
ACTCTAGTAT TGAAAGAGAT GTTGAAGATC GACAGCTCCG GAGCAAGTTC CTCTGCCCCT 
GATTCTGGCG GCCCCAATGC CCAGCAGCAG TGCAAGAGAC GCTCCAGCAA GAAACTAGCT 
GCTCGGATAA ACACTCCTCA GCCTGAAACA GCTCCGGCTG CTGCTGCTGC TCCTCCTCCT 
CCTCCTCCTC CTCATCCTCT GGCTGGTCAG CCACCCCTCC TGCCCAGCAT GGCCACTGAA 
CTAGGACGGG TGTGCATGGG GCTCCGGATG GGACCTCCAG AGTTTGCCTT CCTCCGCAAC 
AGACAGAGTC TGATCTTGTG TCAGGTGAAG TTGTCGAATG GTCTGATGGT TCACGGCCCT 
CAGTGTCAGT CAGAAACCGA AGCTAAAGAG AAAGCTGCTC TCTTCACCCT TCAACGATTG 
AACTCGCTGG GCTCTTTCCC ATTGCCTCCT CCCATATTCC CTGGAGTCCA GCAGATGAGA 
CCGCTTGCAC CTCATCCCTC CATGTTCGGA CAGCCAGGAG GTGGGTTTCT GATGCCCCCT 
CAAGGGTACG GGCCTCTTCC CTGGGGGATG CAGTTTCCCC ATCAGGGGCA GCCGTTCTTT 
GGAGGAACTT TTCCTGGAGC ACGAGGCCCT GTGCCATCAG CAGCCGGCGG ATCCCATAAC 
CAGTTTGTTC CCCTTCAGGT GACCAAGAAA CGAGTTTCAG GCAGAACCAA GAACCAAGAA 
TCAAATCAAG CTCAGAGTGG CTCACCCTAT GGCGTTCAGA CCCCTGCAGT GCCTCCATCA 
GCCCCTAAAA CTCCACCATC CACCAACGCC CACGAGCATG CAGAAAAGCC CACCGCAACT 
ACACCCAAGA CCCCCAAGCA GAATGCCAAT CCCCACACCC CCGGCTCCGC ATCCAAGAGG 
AAACACCGGA AACTGGCAGT CAACTTTGAG GCTGCTAAGG TGACGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

xrn1 ( XM_005163318.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163375.1 Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X2, mRNA.

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CloneID	ODa06836
Clone ID Related Accession (Same CDS sequence)	XM_005163318.3 , XM_005163318.4
Accession Version	XM_005163318.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	5'-3' exoribonuclease 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163318.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTGC CTAAGTTTTA TCGATGGATA TCAGAGCGGT ATCCGTGTCT GAGTGAAGTT 
GTCAAAGAGC ATCAGATCCC AGAGTTCGAC AACCTGTATT TAGACATGAA CGGGATCATC 
CACCAGTGTT CGCACCCAAA TGATGAGGAT GTGCACTTCC GTATTTCTGA AGAGAAGATA 
TTCGCTGACA TTTTTCACTA TCTGGAAGTG CTCTTTCGCA TCATTAAGCC AAGGAAAGTG 
TTCTTCATGG CCGTCGATGG AGTCGCACCC AGAGCCAAAA TGAACCAGCA GCGTGGGAGA 
AGATTCAGGT CAGCAAAAGA GGCAGAGGAG AAAATCAAGA AGGCCTTGGA GAAGGGAGAA 
GTTTTGCCCA CAGAGGCCCG ATTTGACTCT AACTGCATCA CTCCGGGAAC GGATTTCATG 
GCCAGGCTAC AGGAACAGCT TAAATATTTC GTTCACAATA AACTGTCCAC AGACAAAACA 
TGGCAGGGAG TGAATGTGTA TTTGTCTGGT CATGAGACCC CTGGTGAAGG AGAGCACAAA 
ATCATGGAGT TCATTCGCTC TGAGAGCACC AAACCGGGTC ATAACCCAAA CACAAGACAC 
TGCCTTTATG GCCTGGATGC CGATCTGATA ATGCTTGGCT TGACCAGCCA TGAGCCTCAT 
TTTTCTCTCC TCAGAGAGGA GGTTCGCTTT GGAGGCAAAA AGTCACAAAA GAGGATAACA 
GCTCCTGAGG AGACGACCTT TCATCTGCTT CATCTCTCCC TTATGAGGGA ATACATTGAC 
TATGAGTTTT CAGAGCTCAG GAAAAAGATC TCATTTGAGT ATGACCTGGA GCGGATCATA 
GACGACTGGG TTCTGATGGG TTTCCTGGTG GGGAATGACT TCATCCCTCA TCTTCCTCAC 
CTGCACATCA ATCATGACGC ACTCCCGCTG CTCTATCGCA CCTACATTAA TGTGCTGCCA 
AGTCTAGGAG GATACCTGAA TGAAAACGGA CACCTCAACC TCACCCACTT TGAGAAGTAC 
CTAGAGAAGT TATCAGATTT TGACCGGGAG CACTTCAGTG AGGTGTTTGT GGACCTGAAG 
TGGTTTGAGA GTAAGGTGGG GAACAAATAT GTGAACGAAG CTGCGGGGCT GGCAGCTGAG 
GAGGCTGATG GCAGAGACTT GAAGAAGAGT AAATCTTCAG ACGACTCTCT GTGTCTCGCG 
GCTCTTGATG GAGTCAGAGA TTCTCACAGC ACTGCAGGAA GAGCAGAAGG CGCAGAGGAG 
GACGGAGAGG ACGATGACAT GTTTGAAACA GAATTTCGAC AATATAAACG CACCTACTAC 
ATGACCAAAA TGGGTGTTGA AGTCGTCTCA GATGAGTTTT TGGCCAATCA GGCTAAATGT 
TACGTAGAGG GAATCCAGTG GATCTTGCAT TACTACTATC ACGGAGTTCA GTCTTGGAGC 
TGGTATTATC CATACCACTA CGCTCCTTTC CTCTCTGACG TGAGGAACAT CTCTGGTCTC 
TCGCTGACCT TTGACCTGGC CAAACCCTTT ATGCCGTTTG AGCAGCTCTT GGCCGTCCTG 
CCATCAGCCA GCAAAGACCT TCTTCCTCAG TGCTACCAGC ATCTGATGAC GTCACTGAGC 
TCCCCCATTG TCGAGTATTA CCCACAGGAC TTCAAAACTG ACCTGAACGG CAAACAGCAG 
GAGTGGGAAG CAGTGGTGCT CATCCCGTTC ATTGACGAGA AATGCTTGTT AGCAGCTATG 
GAGCCCTACA CTCATAATCT GACCCCATCG GAGAAGGCCA GGAACAGGCA TACGGAGTGT 
GCAGTTTACT GCTACGATAA AGACCTGGAT TATCCCTATA ATTCCCCAGA TCCGCAGATC 
TTCCCCAATA TCGTCCACTG CCATGCCAGG CAAACACCGG TCCCGATGGA TGCCTGGCGT 
GTATCACCTG ATCATGTGCG ATGCAGGATC GACAGAGGAG CGCTTTACTT CTGCGGCTTC 
CCCACATTAC AGCACATCCG TCATAAATTT TACAAGAAGA AAAGTGGAGT GATGGTGTTT 
CAACAAAGCA GCAGAGGAGA AAACATGATC CTGGATATTT TGCCAAGCCA TGAGATGGTG 
CAGGTGGAAG AGGTTGCAGC GCTGGTTCTT GGAAGATCTT TGTTTGTGAA CTGGCCACAT 
TTGATGGAAG CTCGGGTTGT AGCCGTTTCA GATGGAGAGA CAAAATTTTC ATTAGAGGAG 
CCTGCAGGAT CACAGGTGTT GTACATGGAA AACAATCCAC CACCCACTAA AGTCAGCCGT 
GTGACTGATA AAGAGCAGAA GGACTGGGTC AAAGAAGTGC AAGGCCTCAC AGAGCATTTT 
AGCAAGCGTA AAGGCATAGC AATCAATGAG ACGGATGTGT TGGTTTACGG CCAGCTGCTG 
ACAGGCAGGA AGTATGTCAT CGGTCAGAAC GGAAAAGTCC ATTTGGAGAA GCAGTTTGCC 
AAGCAGGTCC TGCCCTTTCC ATATCAGACC ATTGTGAAGG ATATCAAGGC CTTTGATTCC 
TCATTGGCGC GCTTCAAAAC ACTGGAGGAG CTGTTTCCTC TGGGAACCAC AGTCTTTATG 
GTTGGAAATC CATATTACGG TGCAATGGGA GAAGTGCAAG ACTCTAGTGA TGTCATCAGT 
GAGGGACGAG TCAGAGTGGT TTTCTCTGTA CCGTGTGAAC CTCAGCTCGA TGCCTTAATA 
CAGAATCAGC ATAAATACTC TGTGAAGTAC AGTCCCGGGT ATGTACTCGC CTCACGCCTC 
GGTATCACTG GCTACCTCGT CTCAAGGTTC TCTGGTAGCA TCTTCATCGG AAGAGGATCC 
AAGAAAAATC CACATGGAGA GCAGAAAGTC AACGTAGGCC TTAATCTCAA GTTCAACAAA 
AAGAACGAGG AAGTTCCTGG CTACACCAAG AGAACAGAGA AGGAGTGGCT CTACTCTGCT 
GCTGTTGAGG AACTAATAGC TGAATACCTA GAACGATTTT CAGAGGTGTT TGACCATGTA 
GCACGAAACA GCCACGATGA CATATTTTAC GAAGACGACA TCTGGCCGGG AGAGGATGAG 
AATGGAGTGG AAAAGGTCCA AGAGATCCAG GGCTGGTTGA AAACGCATCC AGTGAGCTCC 
ATCTCCAGAG CATCATGTGA TCTGCAGATC CTGGATGCTG GTATTGTGGA GAAGATTGAG 
GAGGAGCTGG AGAAAACGAA GGCAAAGAAG ATGAACAAGA AAGTGCGGGT CACCGTGAAG 
CCCCATCTGC TTTTTAGGCC TCTGGAGCAG CAGCACGGTG TTGTTCCAGA TCCTGATTCA 
GAATATCATC TGTTTGACCG TGTGGTCAAT GTGAGAGAGA GCTTTTCTGT TCCACTCGGC 
CTTCGAGGAA CCATCATCGG CATCAAAGGA GCTGACCGTG AGGCTGAAAT TCTATATGAG 
GTGATATTTG ATGAGGAGTT TGCTGGAGGA CTCACTATCA GGTGTTCTCC TGGCCGTGGG 
TATCGTTTGC CCCCTAGTGC CTTAATCAAC CTGAGCCATG GCAGCAGGAA GGAGCTGGGC 
TCACACAAAC TCACTGCCAT CGTCAAACCT CAACCCTCAT CTTCATCACA AATCTCAAAC 
AAGAGCCAGC TGGGTGGCCT TAATCACTCA CCACGCTCAC CGTTCATACC TACACAGCAG 
AATGGCAGAC AAAGTTTTAA TCTCAGGGGT GACACTCAGG GCAACAGGAA CTCGCCACAT 
AAAGCCCTCA ACCAGAGAAA CCAACAAAAG GGGTCTAGTA ATGAGAAAGA GTTCAGTAAT 
GTATGGCAGT CCCTTCAGAA TTCAGCAATG CCCCAGAAAC CACCAGCCCA CTGGCAAAAT 
AATGCTGGTA AAAGCCAGAT GCCACATCAG AATCAGCTGG AAGGACAAAA TAAACAGAGC 
CAAGGCAACG ACACATCCAT CAAACTTGGT TTGGGTGGTA ATATTAGACT CCTGAAGAGA 
AATGAGGATC TAAATACCGC TCTGCAGGGG CCTTCAAATA AGGCATCTAC AGAGTTTGAG 
GAGCTTATAG CCAATCTTAA AATCTCAAAG GATCCTGAAC CTCCTCAGCA TGAACCACCT 
AAAGAGCCCA AGGCCCCGTC AGCAGAGCCT CTCTCCCCAC AATCCTTTGC CATGAAGGGA 
ACTCTAGTAT TGAAAGAGAT GTTGAAGATC GACAGCTCCG GAGCAAGTTC CTCTGCCCCT 
GATTCTGGCG GCCCCAATGC CCAGCAGCAG TGCAAGAGAC GCTCCAGCAA GAAACTAGCT 
GCTCGGATAA ACACTCCTCA GCCTGAAACA GCTCCGGCTG CTGCTGCTGC TCCTCCTCCT 
CCTCCTCCTC CTCATCCTCT GGCTGGTCAG CCACCCCTCC TGCCCAGCAT GGCCACTGAA 
CTAGGACGGG TGTGCATGGG GCTCCGGATG GGACCTCCAG AGTTTGCCTT CCTCCGCAAC 
AGACAGAGTC TGATCTTGTG TCAGGTGAAG TTGTCGAATG GTCTGATGGT TCACGGCCCT 
CAGTGTCAGT CAGAAACCGA AGCTAAAGAG AAAGCTGCTC TCTTCACCCT TCAACGATTG 
AACTCGCTGG GCTCTTTCCC ATTGCCTCCT CCCATATTCC CTGGAGTCCA GCAGATGAGA 
CCGCTTGCAC CTCATCCCTC CATGTTCGGA CAGCCAGGAG GTGGGTTTCT GATGCCCCCT 
CAAGGGTACG GGCCTCTTCC CTGGGGGATG CAGTTTCCCC ATCAGGGGCA GCCGTTCTTT 
GGAGGAACTT TTCCTGGAGC ACGAGGCCCT GTGCCATCAG CAGCCGGCGG ATCCCATAAC 
CAGTTTGTTC CCCTTCAGGT GACCAAGAAA CGAGTTTCAG GCAGAACCAA GAACCAAGAA 
TCAAATCAAG CTCAGAGTGG CTCACCCTAT GGCGTTCAGA CCCCTGCAGT GCCTCCATCA 
GCCCCTAAAA CTCCACCATC CACCAACGCC CACGAGCATG CAGAAAAGCC CACCGCAACT 
ACACCCAAGA CCCCCAAGCA GAATGCCAAT CCCCACACCC CCGGCTCCGC ATCCAAGAGG 
AAACACCGGA AACTGGCAGT CAACTTTGAG GCTGCTAAGG TGACGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163375.1
CDS	235..5325
Translation	MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDEDVHFRISEEKIFADIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEEKIKKALEKGEVLPTEARFDSNCITPGTDFMARLQEQLKYFVHNKLSTDKTWQGVNVYLSGHETPGEGEHKIMEFIRSESTKPGHNPNTRHCLYGLDADLIMLGLTSHEPHFSLLREEVRFGGKKSQKRITAPEETTFHLLHLSLMREYIDYEFSELRKKISFEYDLERIIDDWVLMGFLVGNDFIPHLPHLHINHDALPLLYRTYINVLPSLGGYLNENGHLNLTHFEKYLEKLSDFDREHFSEVFVDLKWFESKVGNKYVNEAAGLAAEEADGRDLKKSKSSDDSLCLAALDGVRDSHSTAGRAEGAEEDGEDDDMFETEFRQYKRTYYMTKMGVEVVSDEFLANQAKCYVEGIQWILHYYYHGVQSWSWYYPYHYAPFLSDVRNISGLSLTFDLAKPFMPFEQLLAVLPSASKDLLPQCYQHLMTSLSSPIVEYYPQDFKTDLNGKQQEWEAVVLIPFIDEKCLLAAMEPYTHNLTPSEKARNRHTECAVYCYDKDLDYPYNSPDPQIFPNIVHCHARQTPVPMDAWRVSPDHVRCRIDRGALYFCGFPTLQHIRHKFYKKKSGVMVFQQSSRGENMILDILPSHEMVQVEEVAALVLGRSLFVNWPHLMEARVVAVSDGETKFSLEEPAGSQVLYMENNPPPTKVSRVTDKEQKDWVKEVQGLTEHFSKRKGIAINETDVLVYGQLLTGRKYVIGQNGKVHLEKQFAKQVLPFPYQTIVKDIKAFDSSLARFKTLEELFPLGTTVFMVGNPYYGAMGEVQDSSDVISEGRVRVVFSVPCEPQLDALIQNQHKYSVKYSPGYVLASRLGITGYLVSRFSGSIFIGRGSKKNPHGEQKVNVGLNLKFNKKNEEVPGYTKRTEKEWLYSAAVEELIAEYLERFSEVFDHVARNSHDDIFYEDDIWPGEDENGVEKVQEIQGWLKTHPVSSISRASCDLQILDAGIVEKIEEELEKTKAKKMNKKVRVTVKPHLLFRPLEQQHGVVPDPDSEYHLFDRVVNVRESFSVPLGLRGTIIGIKGADREAEILYEVIFDEEFAGGLTIRCSPGRGYRLPPSALINLSHGSRKELGSHKLTAIVKPQPSSSSQISNKSQLGGLNHSPRSPFIPTQQNGRQSFNLRGDTQGNRNSPHKALNQRNQQKGSSNEKEFSNVWQSLQNSAMPQKPPAHWQNNAGKSQMPHQNQLEGQNKQSQGNDTSIKLGLGGNIRLLKRNEDLNTALQGPSNKASTEFEELIANLKISKDPEPPQHEPPKEPKAPSAEPLSPQSFAMKGTLVLKEMLKIDSSGASSSAPDSGGPNAQQQCKRRSSKKLAARINTPQPETAPAAAAAPPPPPPPHPLAGQPPLLPSMATELGRVCMGLRMGPPEFAFLRNRQSLILCQVKLSNGLMVHGPQCQSETEAKEKAALFTLQRLNSLGSFPLPPPIFPGVQQMRPLAPHPSMFGQPGGGFLMPPQGYGPLPWGMQFPHQGQPFFGGTFPGARGPVPSAAGGSHNQFVPLQVTKKRVSGRTKNQESNQAQSGSPYGVQTPAVPPSAPKTPPSTNAHEHAEKPTATTPKTPKQNANPHTPGSASKRKHRKLAVNFEAAKVTD

Target ORF information:

RefSeq Version	XM_005163318.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163318.3

ORF Insert Sequence:

ATGGGAGTGC CTAAGTTTTA TCGATGGATA TCAGAGCGGT ATCCGTGTCT GAGTGAAGTT 
GTCAAAGAGC ATCAGATCCC AGAGTTCGAC AACCTGTATT TAGACATGAA CGGGATCATC 
CACCAGTGTT CGCACCCAAA TGATGAGGAT GTGCACTTCC GTATTTCTGA AGAGAAGATA 
TTCGCTGACA TTTTTCACTA TCTGGAAGTG CTCTTTCGCA TCATTAAGCC AAGGAAAGTG 
TTCTTCATGG CCGTCGATGG AGTCGCACCC AGAGCCAAAA TGAACCAGCA GCGTGGGAGA 
AGATTCAGGT CAGCAAAAGA GGCAGAGGAG AAAATCAAGA AGGCCTTGGA GAAGGGAGAA 
GTTTTGCCCA CAGAGGCCCG ATTTGACTCT AACTGCATCA CTCCGGGAAC GGATTTCATG 
GCCAGGCTAC AGGAACAGCT TAAATATTTC GTTCACAATA AACTGTCCAC AGACAAAACA 
TGGCAGGGAG TGAATGTGTA TTTGTCTGGT CATGAGACCC CTGGTGAAGG AGAGCACAAA 
ATCATGGAGT TCATTCGCTC TGAGAGCACC AAACCGGGTC ATAACCCAAA CACAAGACAC 
TGCCTTTATG GCCTGGATGC CGATCTGATA ATGCTTGGCT TGACCAGCCA TGAGCCTCAT 
TTTTCTCTCC TCAGAGAGGA GGTTCGCTTT GGAGGCAAAA AGTCACAAAA GAGGATAACA 
GCTCCTGAGG AGACGACCTT TCATCTGCTT CATCTCTCCC TTATGAGGGA ATACATTGAC 
TATGAGTTTT CAGAGCTCAG GAAAAAGATC TCATTTGAGT ATGACCTGGA GCGGATCATA 
GACGACTGGG TTCTGATGGG TTTCCTGGTG GGGAATGACT TCATCCCTCA TCTTCCTCAC 
CTGCACATCA ATCATGACGC ACTCCCGCTG CTCTATCGCA CCTACATTAA TGTGCTGCCA 
AGTCTAGGAG GATACCTGAA TGAAAACGGA CACCTCAACC TCACCCACTT TGAGAAGTAC 
CTAGAGAAGT TATCAGATTT TGACCGGGAG CACTTCAGTG AGGTGTTTGT GGACCTGAAG 
TGGTTTGAGA GTAAGGTGGG GAACAAATAT GTGAACGAAG CTGCGGGGCT GGCAGCTGAG 
GAGGCTGATG GCAGAGACTT GAAGAAGAGT AAATCTTCAG ACGACTCTCT GTGTCTCGCG 
GCTCTTGATG GAGTCAGAGA TTCTCACAGC ACTGCAGGAA GAGCAGAAGG CGCAGAGGAG 
GACGGAGAGG ACGATGACAT GTTTGAAACA GAATTTCGAC AATATAAACG CACCTACTAC 
ATGACCAAAA TGGGTGTTGA AGTCGTCTCA GATGAGTTTT TGGCCAATCA GGCTAAATGT 
TACGTAGAGG GAATCCAGTG GATCTTGCAT TACTACTATC ACGGAGTTCA GTCTTGGAGC 
TGGTATTATC CATACCACTA CGCTCCTTTC CTCTCTGACG TGAGGAACAT CTCTGGTCTC 
TCGCTGACCT TTGACCTGGC CAAACCCTTT ATGCCGTTTG AGCAGCTCTT GGCCGTCCTG 
CCATCAGCCA GCAAAGACCT TCTTCCTCAG TGCTACCAGC ATCTGATGAC GTCACTGAGC 
TCCCCCATTG TCGAGTATTA CCCACAGGAC TTCAAAACTG ACCTGAACGG CAAACAGCAG 
GAGTGGGAAG CAGTGGTGCT CATCCCGTTC ATTGACGAGA AATGCTTGTT AGCAGCTATG 
GAGCCCTACA CTCATAATCT GACCCCATCG GAGAAGGCCA GGAACAGGCA TACGGAGTGT 
GCAGTTTACT GCTACGATAA AGACCTGGAT TATCCCTATA ATTCCCCAGA TCCGCAGATC 
TTCCCCAATA TCGTCCACTG CCATGCCAGG CAAACACCGG TCCCGATGGA TGCCTGGCGT 
GTATCACCTG ATCATGTGCG ATGCAGGATC GACAGAGGAG CGCTTTACTT CTGCGGCTTC 
CCCACATTAC AGCACATCCG TCATAAATTT TACAAGAAGA AAAGTGGAGT GATGGTGTTT 
CAACAAAGCA GCAGAGGAGA AAACATGATC CTGGATATTT TGCCAAGCCA TGAGATGGTG 
CAGGTGGAAG AGGTTGCAGC GCTGGTTCTT GGAAGATCTT TGTTTGTGAA CTGGCCACAT 
TTGATGGAAG CTCGGGTTGT AGCCGTTTCA GATGGAGAGA CAAAATTTTC ATTAGAGGAG 
CCTGCAGGAT CACAGGTGTT GTACATGGAA AACAATCCAC CACCCACTAA AGTCAGCCGT 
GTGACTGATA AAGAGCAGAA GGACTGGGTC AAAGAAGTGC AAGGCCTCAC AGAGCATTTT 
AGCAAGCGTA AAGGCATAGC AATCAATGAG ACGGATGTGT TGGTTTACGG CCAGCTGCTG 
ACAGGCAGGA AGTATGTCAT CGGTCAGAAC GGAAAAGTCC ATTTGGAGAA GCAGTTTGCC 
AAGCAGGTCC TGCCCTTTCC ATATCAGACC ATTGTGAAGG ATATCAAGGC CTTTGATTCC 
TCATTGGCGC GCTTCAAAAC ACTGGAGGAG CTGTTTCCTC TGGGAACCAC AGTCTTTATG 
GTTGGAAATC CATATTACGG TGCAATGGGA GAAGTGCAAG ACTCTAGTGA TGTCATCAGT 
GAGGGACGAG TCAGAGTGGT TTTCTCTGTA CCGTGTGAAC CTCAGCTCGA TGCCTTAATA 
CAGAATCAGC ATAAATACTC TGTGAAGTAC AGTCCCGGGT ATGTACTCGC CTCACGCCTC 
GGTATCACTG GCTACCTCGT CTCAAGGTTC TCTGGTAGCA TCTTCATCGG AAGAGGATCC 
AAGAAAAATC CACATGGAGA GCAGAAAGTC AACGTAGGCC TTAATCTCAA GTTCAACAAA 
AAGAACGAGG AAGTTCCTGG CTACACCAAG AGAACAGAGA AGGAGTGGCT CTACTCTGCT 
GCTGTTGAGG AACTAATAGC TGAATACCTA GAACGATTTT CAGAGGTGTT TGACCATGTA 
GCACGAAACA GCCACGATGA CATATTTTAC GAAGACGACA TCTGGCCGGG AGAGGATGAG 
AATGGAGTGG AAAAGGTCCA AGAGATCCAG GGCTGGTTGA AAACGCATCC AGTGAGCTCC 
ATCTCCAGAG CATCATGTGA TCTGCAGATC CTGGATGCTG GTATTGTGGA GAAGATTGAG 
GAGGAGCTGG AGAAAACGAA GGCAAAGAAG ATGAACAAGA AAGTGCGGGT CACCGTGAAG 
CCCCATCTGC TTTTTAGGCC TCTGGAGCAG CAGCACGGTG TTGTTCCAGA TCCTGATTCA 
GAATATCATC TGTTTGACCG TGTGGTCAAT GTGAGAGAGA GCTTTTCTGT TCCACTCGGC 
CTTCGAGGAA CCATCATCGG CATCAAAGGA GCTGACCGTG AGGCTGAAAT TCTATATGAG 
GTGATATTTG ATGAGGAGTT TGCTGGAGGA CTCACTATCA GGTGTTCTCC TGGCCGTGGG 
TATCGTTTGC CCCCTAGTGC CTTAATCAAC CTGAGCCATG GCAGCAGGAA GGAGCTGGGC 
TCACACAAAC TCACTGCCAT CGTCAAACCT CAACCCTCAT CTTCATCACA AATCTCAAAC 
AAGAGCCAGC TGGGTGGCCT TAATCACTCA CCACGCTCAC CGTTCATACC TACACAGCAG 
AATGGCAGAC AAAGTTTTAA TCTCAGGGGT GACACTCAGG GCAACAGGAA CTCGCCACAT 
AAAGCCCTCA ACCAGAGAAA CCAACAAAAG GGGTCTAGTA ATGAGAAAGA GTTCAGTAAT 
GTATGGCAGT CCCTTCAGAA TTCAGCAATG CCCCAGAAAC CACCAGCCCA CTGGCAAAAT 
AATGCTGGTA AAAGCCAGAT GCCACATCAG AATCAGCTGG AAGGACAAAA TAAACAGAGC 
CAAGGCAACG ACACATCCAT CAAACTTGGT TTGGGTGGTA ATATTAGACT CCTGAAGAGA 
AATGAGGATC TAAATACCGC TCTGCAGGGG CCTTCAAATA AGGCATCTAC AGAGTTTGAG 
GAGCTTATAG CCAATCTTAA AATCTCAAAG GATCCTGAAC CTCCTCAGCA TGAACCACCT 
AAAGAGCCCA AGGCCCCGTC AGCAGAGCCT CTCTCCCCAC AATCCTTTGC CATGAAGGGA 
ACTCTAGTAT TGAAAGAGAT GTTGAAGATC GACAGCTCCG GAGCAAGTTC CTCTGCCCCT 
GATTCTGGCG GCCCCAATGC CCAGCAGCAG TGCAAGAGAC GCTCCAGCAA GAAACTAGCT 
GCTCGGATAA ACACTCCTCA GCCTGAAACA GCTCCGGCTG CTGCTGCTGC TCCTCCTCCT 
CCTCCTCCTC CTCATCCTCT GGCTGGTCAG CCACCCCTCC TGCCCAGCAT GGCCACTGAA 
CTAGGACGGG TGTGCATGGG GCTCCGGATG GGACCTCCAG AGTTTGCCTT CCTCCGCAAC 
AGACAGAGTC TGATCTTGTG TCAGGTGAAG TTGTCGAATG GTCTGATGGT TCACGGCCCT 
CAGTGTCAGT CAGAAACCGA AGCTAAAGAG AAAGCTGCTC TCTTCACCCT TCAACGATTG 
AACTCGCTGG GCTCTTTCCC ATTGCCTCCT CCCATATTCC CTGGAGTCCA GCAGATGAGA 
CCGCTTGCAC CTCATCCCTC CATGTTCGGA CAGCCAGGAG GTGGGTTTCT GATGCCCCCT 
CAAGGGTACG GGCCTCTTCC CTGGGGGATG CAGTTTCCCC ATCAGGGGCA GCCGTTCTTT 
GGAGGAACTT TTCCTGGAGC ACGAGGCCCT GTGCCATCAG CAGCCGGCGG ATCCCATAAC 
CAGTTTGTTC CCCTTCAGGT GACCAAGAAA CGAGTTTCAG GCAGAACCAA GAACCAAGAA 
TCAAATCAAG CTCAGAGTGG CTCACCCTAT GGCGTTCAGA CCCCTGCAGT GCCTCCATCA 
GCCCCTAAAA CTCCACCATC CACCAACGCC CACGAGCATG CAGAAAAGCC CACCGCAACT 
ACACCCAAGA CCCCCAAGCA GAATGCCAAT CCCCACACCC CCGGCTCCGC ATCCAAGAGG 
AAACACCGGA AACTGGCAGT CAACTTTGAG GCTGCTAAGG TGACGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

xrn1 ( XM_005163318.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163375.1 Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X2, mRNA.

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CloneID	ODa06836
Clone ID Related Accession (Same CDS sequence)	XM_005163318.3 , XM_005163318.4
Accession Version	XM_005163318.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	5'-3' exoribonuclease 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163318.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTGC CTAAGTTTTA TCGATGGATA TCAGAGCGGT ATCCGTGTCT GAGTGAAGTT 
GTCAAAGAGC ATCAGATCCC AGAGTTCGAC AACCTGTATT TAGACATGAA CGGGATCATC 
CACCAGTGTT CGCACCCAAA TGATGAGGAT GTGCACTTCC GTATTTCTGA AGAGAAGATA 
TTCGCTGACA TTTTTCACTA TCTGGAAGTG CTCTTTCGCA TCATTAAGCC AAGGAAAGTG 
TTCTTCATGG CCGTCGATGG AGTCGCACCC AGAGCCAAAA TGAACCAGCA GCGTGGGAGA 
AGATTCAGGT CAGCAAAAGA GGCAGAGGAG AAAATCAAGA AGGCCTTGGA GAAGGGAGAA 
GTTTTGCCCA CAGAGGCCCG ATTTGACTCT AACTGCATCA CTCCGGGAAC GGATTTCATG 
GCCAGGCTAC AGGAACAGCT TAAATATTTC GTTCACAATA AACTGTCCAC AGACAAAACA 
TGGCAGGGAG TGAATGTGTA TTTGTCTGGT CATGAGACCC CTGGTGAAGG AGAGCACAAA 
ATCATGGAGT TCATTCGCTC TGAGAGCACC AAACCGGGTC ATAACCCAAA CACAAGACAC 
TGCCTTTATG GCCTGGATGC CGATCTGATA ATGCTTGGCT TGACCAGCCA TGAGCCTCAT 
TTTTCTCTCC TCAGAGAGGA GGTTCGCTTT GGAGGCAAAA AGTCACAAAA GAGGATAACA 
GCTCCTGAGG AGACGACCTT TCATCTGCTT CATCTCTCCC TTATGAGGGA ATACATTGAC 
TATGAGTTTT CAGAGCTCAG GAAAAAGATC TCATTTGAGT ATGACCTGGA GCGGATCATA 
GACGACTGGG TTCTGATGGG TTTCCTGGTG GGGAATGACT TCATCCCTCA TCTTCCTCAC 
CTGCACATCA ATCATGACGC ACTCCCGCTG CTCTATCGCA CCTACATTAA TGTGCTGCCA 
AGTCTAGGAG GATACCTGAA TGAAAACGGA CACCTCAACC TCACCCACTT TGAGAAGTAC 
CTAGAGAAGT TATCAGATTT TGACCGGGAG CACTTCAGTG AGGTGTTTGT GGACCTGAAG 
TGGTTTGAGA GTAAGGTGGG GAACAAATAT GTGAACGAAG CTGCGGGGCT GGCAGCTGAG 
GAGGCTGATG GCAGAGACTT GAAGAAGAGT AAATCTTCAG ACGACTCTCT GTGTCTCGCG 
GCTCTTGATG GAGTCAGAGA TTCTCACAGC ACTGCAGGAA GAGCAGAAGG CGCAGAGGAG 
GACGGAGAGG ACGATGACAT GTTTGAAACA GAATTTCGAC AATATAAACG CACCTACTAC 
ATGACCAAAA TGGGTGTTGA AGTCGTCTCA GATGAGTTTT TGGCCAATCA GGCTAAATGT 
TACGTAGAGG GAATCCAGTG GATCTTGCAT TACTACTATC ACGGAGTTCA GTCTTGGAGC 
TGGTATTATC CATACCACTA CGCTCCTTTC CTCTCTGACG TGAGGAACAT CTCTGGTCTC 
TCGCTGACCT TTGACCTGGC CAAACCCTTT ATGCCGTTTG AGCAGCTCTT GGCCGTCCTG 
CCATCAGCCA GCAAAGACCT TCTTCCTCAG TGCTACCAGC ATCTGATGAC GTCACTGAGC 
TCCCCCATTG TCGAGTATTA CCCACAGGAC TTCAAAACTG ACCTGAACGG CAAACAGCAG 
GAGTGGGAAG CAGTGGTGCT CATCCCGTTC ATTGACGAGA AATGCTTGTT AGCAGCTATG 
GAGCCCTACA CTCATAATCT GACCCCATCG GAGAAGGCCA GGAACAGGCA TACGGAGTGT 
GCAGTTTACT GCTACGATAA AGACCTGGAT TATCCCTATA ATTCCCCAGA TCCGCAGATC 
TTCCCCAATA TCGTCCACTG CCATGCCAGG CAAACACCGG TCCCGATGGA TGCCTGGCGT 
GTATCACCTG ATCATGTGCG ATGCAGGATC GACAGAGGAG CGCTTTACTT CTGCGGCTTC 
CCCACATTAC AGCACATCCG TCATAAATTT TACAAGAAGA AAAGTGGAGT GATGGTGTTT 
CAACAAAGCA GCAGAGGAGA AAACATGATC CTGGATATTT TGCCAAGCCA TGAGATGGTG 
CAGGTGGAAG AGGTTGCAGC GCTGGTTCTT GGAAGATCTT TGTTTGTGAA CTGGCCACAT 
TTGATGGAAG CTCGGGTTGT AGCCGTTTCA GATGGAGAGA CAAAATTTTC ATTAGAGGAG 
CCTGCAGGAT CACAGGTGTT GTACATGGAA AACAATCCAC CACCCACTAA AGTCAGCCGT 
GTGACTGATA AAGAGCAGAA GGACTGGGTC AAAGAAGTGC AAGGCCTCAC AGAGCATTTT 
AGCAAGCGTA AAGGCATAGC AATCAATGAG ACGGATGTGT TGGTTTACGG CCAGCTGCTG 
ACAGGCAGGA AGTATGTCAT CGGTCAGAAC GGAAAAGTCC ATTTGGAGAA GCAGTTTGCC 
AAGCAGGTCC TGCCCTTTCC ATATCAGACC ATTGTGAAGG ATATCAAGGC CTTTGATTCC 
TCATTGGCGC GCTTCAAAAC ACTGGAGGAG CTGTTTCCTC TGGGAACCAC AGTCTTTATG 
GTTGGAAATC CATATTACGG TGCAATGGGA GAAGTGCAAG ACTCTAGTGA TGTCATCAGT 
GAGGGACGAG TCAGAGTGGT TTTCTCTGTA CCGTGTGAAC CTCAGCTCGA TGCCTTAATA 
CAGAATCAGC ATAAATACTC TGTGAAGTAC AGTCCCGGGT ATGTACTCGC CTCACGCCTC 
GGTATCACTG GCTACCTCGT CTCAAGGTTC TCTGGTAGCA TCTTCATCGG AAGAGGATCC 
AAGAAAAATC CACATGGAGA GCAGAAAGTC AACGTAGGCC TTAATCTCAA GTTCAACAAA 
AAGAACGAGG AAGTTCCTGG CTACACCAAG AGAACAGAGA AGGAGTGGCT CTACTCTGCT 
GCTGTTGAGG AACTAATAGC TGAATACCTA GAACGATTTT CAGAGGTGTT TGACCATGTA 
GCACGAAACA GCCACGATGA CATATTTTAC GAAGACGACA TCTGGCCGGG AGAGGATGAG 
AATGGAGTGG AAAAGGTCCA AGAGATCCAG GGCTGGTTGA AAACGCATCC AGTGAGCTCC 
ATCTCCAGAG CATCATGTGA TCTGCAGATC CTGGATGCTG GTATTGTGGA GAAGATTGAG 
GAGGAGCTGG AGAAAACGAA GGCAAAGAAG ATGAACAAGA AAGTGCGGGT CACCGTGAAG 
CCCCATCTGC TTTTTAGGCC TCTGGAGCAG CAGCACGGTG TTGTTCCAGA TCCTGATTCA 
GAATATCATC TGTTTGACCG TGTGGTCAAT GTGAGAGAGA GCTTTTCTGT TCCACTCGGC 
CTTCGAGGAA CCATCATCGG CATCAAAGGA GCTGACCGTG AGGCTGAAAT TCTATATGAG 
GTGATATTTG ATGAGGAGTT TGCTGGAGGA CTCACTATCA GGTGTTCTCC TGGCCGTGGG 
TATCGTTTGC CCCCTAGTGC CTTAATCAAC CTGAGCCATG GCAGCAGGAA GGAGCTGGGC 
TCACACAAAC TCACTGCCAT CGTCAAACCT CAACCCTCAT CTTCATCACA AATCTCAAAC 
AAGAGCCAGC TGGGTGGCCT TAATCACTCA CCACGCTCAC CGTTCATACC TACACAGCAG 
AATGGCAGAC AAAGTTTTAA TCTCAGGGGT GACACTCAGG GCAACAGGAA CTCGCCACAT 
AAAGCCCTCA ACCAGAGAAA CCAACAAAAG GGGTCTAGTA ATGAGAAAGA GTTCAGTAAT 
GTATGGCAGT CCCTTCAGAA TTCAGCAATG CCCCAGAAAC CACCAGCCCA CTGGCAAAAT 
AATGCTGGTA AAAGCCAGAT GCCACATCAG AATCAGCTGG AAGGACAAAA TAAACAGAGC 
CAAGGCAACG ACACATCCAT CAAACTTGGT TTGGGTGGTA ATATTAGACT CCTGAAGAGA 
AATGAGGATC TAAATACCGC TCTGCAGGGG CCTTCAAATA AGGCATCTAC AGAGTTTGAG 
GAGCTTATAG CCAATCTTAA AATCTCAAAG GATCCTGAAC CTCCTCAGCA TGAACCACCT 
AAAGAGCCCA AGGCCCCGTC AGCAGAGCCT CTCTCCCCAC AATCCTTTGC CATGAAGGGA 
ACTCTAGTAT TGAAAGAGAT GTTGAAGATC GACAGCTCCG GAGCAAGTTC CTCTGCCCCT 
GATTCTGGCG GCCCCAATGC CCAGCAGCAG TGCAAGAGAC GCTCCAGCAA GAAACTAGCT 
GCTCGGATAA ACACTCCTCA GCCTGAAACA GCTCCGGCTG CTGCTGCTGC TCCTCCTCCT 
CCTCCTCCTC CTCATCCTCT GGCTGGTCAG CCACCCCTCC TGCCCAGCAT GGCCACTGAA 
CTAGGACGGG TGTGCATGGG GCTCCGGATG GGACCTCCAG AGTTTGCCTT CCTCCGCAAC 
AGACAGAGTC TGATCTTGTG TCAGGTGAAG TTGTCGAATG GTCTGATGGT TCACGGCCCT 
CAGTGTCAGT CAGAAACCGA AGCTAAAGAG AAAGCTGCTC TCTTCACCCT TCAACGATTG 
AACTCGCTGG GCTCTTTCCC ATTGCCTCCT CCCATATTCC CTGGAGTCCA GCAGATGAGA 
CCGCTTGCAC CTCATCCCTC CATGTTCGGA CAGCCAGGAG GTGGGTTTCT GATGCCCCCT 
CAAGGGTACG GGCCTCTTCC CTGGGGGATG CAGTTTCCCC ATCAGGGGCA GCCGTTCTTT 
GGAGGAACTT TTCCTGGAGC ACGAGGCCCT GTGCCATCAG CAGCCGGCGG ATCCCATAAC 
CAGTTTGTTC CCCTTCAGGT GACCAAGAAA CGAGTTTCAG GCAGAACCAA GAACCAAGAA 
TCAAATCAAG CTCAGAGTGG CTCACCCTAT GGCGTTCAGA CCCCTGCAGT GCCTCCATCA 
GCCCCTAAAA CTCCACCATC CACCAACGCC CACGAGCATG CAGAAAAGCC CACCGCAACT 
ACACCCAAGA CCCCCAAGCA GAATGCCAAT CCCCACACCC CCGGCTCCGC ATCCAAGAGG 
AAACACCGGA AACTGGCAGT CAACTTTGAG GCTGCTAAGG TGACGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163375.1
CDS	310..5400
Translation	MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDEDVHFRISEEKIFADIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEEKIKKALEKGEVLPTEARFDSNCITPGTDFMARLQEQLKYFVHNKLSTDKTWQGVNVYLSGHETPGEGEHKIMEFIRSESTKPGHNPNTRHCLYGLDADLIMLGLTSHEPHFSLLREEVRFGGKKSQKRITAPEETTFHLLHLSLMREYIDYEFSELRKKISFEYDLERIIDDWVLMGFLVGNDFIPHLPHLHINHDALPLLYRTYINVLPSLGGYLNENGHLNLTHFEKYLEKLSDFDREHFSEVFVDLKWFESKVGNKYVNEAAGLAAEEADGRDLKKSKSSDDSLCLAALDGVRDSHSTAGRAEGAEEDGEDDDMFETEFRQYKRTYYMTKMGVEVVSDEFLANQAKCYVEGIQWILHYYYHGVQSWSWYYPYHYAPFLSDVRNISGLSLTFDLAKPFMPFEQLLAVLPSASKDLLPQCYQHLMTSLSSPIVEYYPQDFKTDLNGKQQEWEAVVLIPFIDEKCLLAAMEPYTHNLTPSEKARNRHTECAVYCYDKDLDYPYNSPDPQIFPNIVHCHARQTPVPMDAWRVSPDHVRCRIDRGALYFCGFPTLQHIRHKFYKKKSGVMVFQQSSRGENMILDILPSHEMVQVEEVAALVLGRSLFVNWPHLMEARVVAVSDGETKFSLEEPAGSQVLYMENNPPPTKVSRVTDKEQKDWVKEVQGLTEHFSKRKGIAINETDVLVYGQLLTGRKYVIGQNGKVHLEKQFAKQVLPFPYQTIVKDIKAFDSSLARFKTLEELFPLGTTVFMVGNPYYGAMGEVQDSSDVISEGRVRVVFSVPCEPQLDALIQNQHKYSVKYSPGYVLASRLGITGYLVSRFSGSIFIGRGSKKNPHGEQKVNVGLNLKFNKKNEEVPGYTKRTEKEWLYSAAVEELIAEYLERFSEVFDHVARNSHDDIFYEDDIWPGEDENGVEKVQEIQGWLKTHPVSSISRASCDLQILDAGIVEKIEEELEKTKAKKMNKKVRVTVKPHLLFRPLEQQHGVVPDPDSEYHLFDRVVNVRESFSVPLGLRGTIIGIKGADREAEILYEVIFDEEFAGGLTIRCSPGRGYRLPPSALINLSHGSRKELGSHKLTAIVKPQPSSSSQISNKSQLGGLNHSPRSPFIPTQQNGRQSFNLRGDTQGNRNSPHKALNQRNQQKGSSNEKEFSNVWQSLQNSAMPQKPPAHWQNNAGKSQMPHQNQLEGQNKQSQGNDTSIKLGLGGNIRLLKRNEDLNTALQGPSNKASTEFEELIANLKISKDPEPPQHEPPKEPKAPSAEPLSPQSFAMKGTLVLKEMLKIDSSGASSSAPDSGGPNAQQQCKRRSSKKLAARINTPQPETAPAAAAAPPPPPPPHPLAGQPPLLPSMATELGRVCMGLRMGPPEFAFLRNRQSLILCQVKLSNGLMVHGPQCQSETEAKEKAALFTLQRLNSLGSFPLPPPIFPGVQQMRPLAPHPSMFGQPGGGFLMPPQGYGPLPWGMQFPHQGQPFFGGTFPGARGPVPSAAGGSHNQFVPLQVTKKRVSGRTKNQESNQAQSGSPYGVQTPAVPPSAPKTPPSTNAHEHAEKPTATTPKTPKQNANPHTPGSASKRKHRKLAVNFEAAKVTD

Target ORF information:

RefSeq Version	XM_005163318.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 5'-3' exoribonuclease 1 (xrn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163318.4

ORF Insert Sequence:

ATGGGAGTGC CTAAGTTTTA TCGATGGATA TCAGAGCGGT ATCCGTGTCT GAGTGAAGTT 
GTCAAAGAGC ATCAGATCCC AGAGTTCGAC AACCTGTATT TAGACATGAA CGGGATCATC 
CACCAGTGTT CGCACCCAAA TGATGAGGAT GTGCACTTCC GTATTTCTGA AGAGAAGATA 
TTCGCTGACA TTTTTCACTA TCTGGAAGTG CTCTTTCGCA TCATTAAGCC AAGGAAAGTG 
TTCTTCATGG CCGTCGATGG AGTCGCACCC AGAGCCAAAA TGAACCAGCA GCGTGGGAGA 
AGATTCAGGT CAGCAAAAGA GGCAGAGGAG AAAATCAAGA AGGCCTTGGA GAAGGGAGAA 
GTTTTGCCCA CAGAGGCCCG ATTTGACTCT AACTGCATCA CTCCGGGAAC GGATTTCATG 
GCCAGGCTAC AGGAACAGCT TAAATATTTC GTTCACAATA AACTGTCCAC AGACAAAACA 
TGGCAGGGAG TGAATGTGTA TTTGTCTGGT CATGAGACCC CTGGTGAAGG AGAGCACAAA 
ATCATGGAGT TCATTCGCTC TGAGAGCACC AAACCGGGTC ATAACCCAAA CACAAGACAC 
TGCCTTTATG GCCTGGATGC CGATCTGATA ATGCTTGGCT TGACCAGCCA TGAGCCTCAT 
TTTTCTCTCC TCAGAGAGGA GGTTCGCTTT GGAGGCAAAA AGTCACAAAA GAGGATAACA 
GCTCCTGAGG AGACGACCTT TCATCTGCTT CATCTCTCCC TTATGAGGGA ATACATTGAC 
TATGAGTTTT CAGAGCTCAG GAAAAAGATC TCATTTGAGT ATGACCTGGA GCGGATCATA 
GACGACTGGG TTCTGATGGG TTTCCTGGTG GGGAATGACT TCATCCCTCA TCTTCCTCAC 
CTGCACATCA ATCATGACGC ACTCCCGCTG CTCTATCGCA CCTACATTAA TGTGCTGCCA 
AGTCTAGGAG GATACCTGAA TGAAAACGGA CACCTCAACC TCACCCACTT TGAGAAGTAC 
CTAGAGAAGT TATCAGATTT TGACCGGGAG CACTTCAGTG AGGTGTTTGT GGACCTGAAG 
TGGTTTGAGA GTAAGGTGGG GAACAAATAT GTGAACGAAG CTGCGGGGCT GGCAGCTGAG 
GAGGCTGATG GCAGAGACTT GAAGAAGAGT AAATCTTCAG ACGACTCTCT GTGTCTCGCG 
GCTCTTGATG GAGTCAGAGA TTCTCACAGC ACTGCAGGAA GAGCAGAAGG CGCAGAGGAG 
GACGGAGAGG ACGATGACAT GTTTGAAACA GAATTTCGAC AATATAAACG CACCTACTAC 
ATGACCAAAA TGGGTGTTGA AGTCGTCTCA GATGAGTTTT TGGCCAATCA GGCTAAATGT 
TACGTAGAGG GAATCCAGTG GATCTTGCAT TACTACTATC ACGGAGTTCA GTCTTGGAGC 
TGGTATTATC CATACCACTA CGCTCCTTTC CTCTCTGACG TGAGGAACAT CTCTGGTCTC 
TCGCTGACCT TTGACCTGGC CAAACCCTTT ATGCCGTTTG AGCAGCTCTT GGCCGTCCTG 
CCATCAGCCA GCAAAGACCT TCTTCCTCAG TGCTACCAGC ATCTGATGAC GTCACTGAGC 
TCCCCCATTG TCGAGTATTA CCCACAGGAC TTCAAAACTG ACCTGAACGG CAAACAGCAG 
GAGTGGGAAG CAGTGGTGCT CATCCCGTTC ATTGACGAGA AATGCTTGTT AGCAGCTATG 
GAGCCCTACA CTCATAATCT GACCCCATCG GAGAAGGCCA GGAACAGGCA TACGGAGTGT 
GCAGTTTACT GCTACGATAA AGACCTGGAT TATCCCTATA ATTCCCCAGA TCCGCAGATC 
TTCCCCAATA TCGTCCACTG CCATGCCAGG CAAACACCGG TCCCGATGGA TGCCTGGCGT 
GTATCACCTG ATCATGTGCG ATGCAGGATC GACAGAGGAG CGCTTTACTT CTGCGGCTTC 
CCCACATTAC AGCACATCCG TCATAAATTT TACAAGAAGA AAAGTGGAGT GATGGTGTTT 
CAACAAAGCA GCAGAGGAGA AAACATGATC CTGGATATTT TGCCAAGCCA TGAGATGGTG 
CAGGTGGAAG AGGTTGCAGC GCTGGTTCTT GGAAGATCTT TGTTTGTGAA CTGGCCACAT 
TTGATGGAAG CTCGGGTTGT AGCCGTTTCA GATGGAGAGA CAAAATTTTC ATTAGAGGAG 
CCTGCAGGAT CACAGGTGTT GTACATGGAA AACAATCCAC CACCCACTAA AGTCAGCCGT 
GTGACTGATA AAGAGCAGAA GGACTGGGTC AAAGAAGTGC AAGGCCTCAC AGAGCATTTT 
AGCAAGCGTA AAGGCATAGC AATCAATGAG ACGGATGTGT TGGTTTACGG CCAGCTGCTG 
ACAGGCAGGA AGTATGTCAT CGGTCAGAAC GGAAAAGTCC ATTTGGAGAA GCAGTTTGCC 
AAGCAGGTCC TGCCCTTTCC ATATCAGACC ATTGTGAAGG ATATCAAGGC CTTTGATTCC 
TCATTGGCGC GCTTCAAAAC ACTGGAGGAG CTGTTTCCTC TGGGAACCAC AGTCTTTATG 
GTTGGAAATC CATATTACGG TGCAATGGGA GAAGTGCAAG ACTCTAGTGA TGTCATCAGT 
GAGGGACGAG TCAGAGTGGT TTTCTCTGTA CCGTGTGAAC CTCAGCTCGA TGCCTTAATA 
CAGAATCAGC ATAAATACTC TGTGAAGTAC AGTCCCGGGT ATGTACTCGC CTCACGCCTC 
GGTATCACTG GCTACCTCGT CTCAAGGTTC TCTGGTAGCA TCTTCATCGG AAGAGGATCC 
AAGAAAAATC CACATGGAGA GCAGAAAGTC AACGTAGGCC TTAATCTCAA GTTCAACAAA 
AAGAACGAGG AAGTTCCTGG CTACACCAAG AGAACAGAGA AGGAGTGGCT CTACTCTGCT 
GCTGTTGAGG AACTAATAGC TGAATACCTA GAACGATTTT CAGAGGTGTT TGACCATGTA 
GCACGAAACA GCCACGATGA CATATTTTAC GAAGACGACA TCTGGCCGGG AGAGGATGAG 
AATGGAGTGG AAAAGGTCCA AGAGATCCAG GGCTGGTTGA AAACGCATCC AGTGAGCTCC 
ATCTCCAGAG CATCATGTGA TCTGCAGATC CTGGATGCTG GTATTGTGGA GAAGATTGAG 
GAGGAGCTGG AGAAAACGAA GGCAAAGAAG ATGAACAAGA AAGTGCGGGT CACCGTGAAG 
CCCCATCTGC TTTTTAGGCC TCTGGAGCAG CAGCACGGTG TTGTTCCAGA TCCTGATTCA 
GAATATCATC TGTTTGACCG TGTGGTCAAT GTGAGAGAGA GCTTTTCTGT TCCACTCGGC 
CTTCGAGGAA CCATCATCGG CATCAAAGGA GCTGACCGTG AGGCTGAAAT TCTATATGAG 
GTGATATTTG ATGAGGAGTT TGCTGGAGGA CTCACTATCA GGTGTTCTCC TGGCCGTGGG 
TATCGTTTGC CCCCTAGTGC CTTAATCAAC CTGAGCCATG GCAGCAGGAA GGAGCTGGGC 
TCACACAAAC TCACTGCCAT CGTCAAACCT CAACCCTCAT CTTCATCACA AATCTCAAAC 
AAGAGCCAGC TGGGTGGCCT TAATCACTCA CCACGCTCAC CGTTCATACC TACACAGCAG 
AATGGCAGAC AAAGTTTTAA TCTCAGGGGT GACACTCAGG GCAACAGGAA CTCGCCACAT 
AAAGCCCTCA ACCAGAGAAA CCAACAAAAG GGGTCTAGTA ATGAGAAAGA GTTCAGTAAT 
GTATGGCAGT CCCTTCAGAA TTCAGCAATG CCCCAGAAAC CACCAGCCCA CTGGCAAAAT 
AATGCTGGTA AAAGCCAGAT GCCACATCAG AATCAGCTGG AAGGACAAAA TAAACAGAGC 
CAAGGCAACG ACACATCCAT CAAACTTGGT TTGGGTGGTA ATATTAGACT CCTGAAGAGA 
AATGAGGATC TAAATACCGC TCTGCAGGGG CCTTCAAATA AGGCATCTAC AGAGTTTGAG 
GAGCTTATAG CCAATCTTAA AATCTCAAAG GATCCTGAAC CTCCTCAGCA TGAACCACCT 
AAAGAGCCCA AGGCCCCGTC AGCAGAGCCT CTCTCCCCAC AATCCTTTGC CATGAAGGGA 
ACTCTAGTAT TGAAAGAGAT GTTGAAGATC GACAGCTCCG GAGCAAGTTC CTCTGCCCCT 
GATTCTGGCG GCCCCAATGC CCAGCAGCAG TGCAAGAGAC GCTCCAGCAA GAAACTAGCT 
GCTCGGATAA ACACTCCTCA GCCTGAAACA GCTCCGGCTG CTGCTGCTGC TCCTCCTCCT 
CCTCCTCCTC CTCATCCTCT GGCTGGTCAG CCACCCCTCC TGCCCAGCAT GGCCACTGAA 
CTAGGACGGG TGTGCATGGG GCTCCGGATG GGACCTCCAG AGTTTGCCTT CCTCCGCAAC 
AGACAGAGTC TGATCTTGTG TCAGGTGAAG TTGTCGAATG GTCTGATGGT TCACGGCCCT 
CAGTGTCAGT CAGAAACCGA AGCTAAAGAG AAAGCTGCTC TCTTCACCCT TCAACGATTG 
AACTCGCTGG GCTCTTTCCC ATTGCCTCCT CCCATATTCC CTGGAGTCCA GCAGATGAGA 
CCGCTTGCAC CTCATCCCTC CATGTTCGGA CAGCCAGGAG GTGGGTTTCT GATGCCCCCT 
CAAGGGTACG GGCCTCTTCC CTGGGGGATG CAGTTTCCCC ATCAGGGGCA GCCGTTCTTT 
GGAGGAACTT TTCCTGGAGC ACGAGGCCCT GTGCCATCAG CAGCCGGCGG ATCCCATAAC 
CAGTTTGTTC CCCTTCAGGT GACCAAGAAA CGAGTTTCAG GCAGAACCAA GAACCAAGAA 
TCAAATCAAG CTCAGAGTGG CTCACCCTAT GGCGTTCAGA CCCCTGCAGT GCCTCCATCA 
GCCCCTAAAA CTCCACCATC CACCAACGCC CACGAGCATG CAGAAAAGCC CACCGCAACT 
ACACCCAAGA CCCCCAAGCA GAATGCCAAT CCCCACACCC CCGGCTCCGC ATCCAAGAGG 
AAACACCGGA AACTGGCAGT CAACTTTGAG GCTGCTAAGG TGACGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequential, Divergent, and Cooperative Requirements of
Genetics205(4)1551-1572(2017 04)
Yang YJ,Wang Y,Li Z,Zhou L,Gui JF

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

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xrn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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