msgn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	msgn1
Entrez Gene ID	360135
Full Name	mesogenin 1
Synonyms	Meso1a,mespo,wu:fc39h09,zgc:109769
General protein information	Preferred Names mesogenin 1 Names mesogenin-1 mesP-related bHLH factor pMeso1a pMesogenin1 pMsgn1 paraxial mesoderm-specific expression and regulatory capacities
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_182882.2	NP_878302.1	mesogenin-1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MSGN1	XP_525696.1
Xenopus tropicalis (tropical clawed frog)	msgn1	NP_001039104.1
Danio rerio (zebrafish)	msgn1	NP_878302.1
Homo sapiens (human)	MSGN1	NP_001099039.1
Rattus norvegicus (Norway rat)	Msgn1	NP_001103021.1
	LOC715386	XP_002808076.1
Canis lupus familiaris (dog)	MSGN1	XP_540094.2
Bos taurus (cattle)	MSGN1	XP_002691567.2
Mus musculus (house mouse)	Msgn1	NP_062417.1
Gallus gallus (chicken)	MSGN1	NP_990015.2

Gene Ontology
Bibliography

Related articles in PubMed

The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation.
Gross-Thebing T, Yigit S, Pfeiffer J, Reichman-Fried M, Bandemer J, Ruckert C, Rathmer C, Goudarzi M, Stehling M, Tarbashevich K, Seggewiss J, Raz E
Developmental cell43(6)704-715.e5(2017 Dec)

tbx6l and tbx16 are redundantly required for posterior paraxial mesoderm formation during zebrafish embryogenesis.
Morrow ZT, Maxwell AM, Hoshijima K, Talbot JC, Grunwald DJ, Amacher SL
Developmental dynamics : an official publication of the American Association of Anatomists246(10)759-769(2017 Oct)

FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step epithelial to mesenchymal transition.
Goto H, Kimmey SC, Row RH, Matus DQ, Martin BL
Development (Cambridge, England)144(8)1412-1424(2017 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tbx16 and Msgn1 are required to establish directional cell migration of zebrafish mesodermal progenitors.(
Title: Tbx16 and Msgn1 are required to establish directional cell migration of zebrafish mesodermal progenitors.

Through its combined effects on gene expression and cell movement, Msgn1 (with Spt) plays a key role both in genesis of the paraxial mesoderm and in maintenance of the progenitor population from which it derives.
Title: The differentiation and movement of presomitic mesoderm progenitor cells are controlled by Mesogenin 1.

Msgn1 expression in association with that of ntl may allow the differentiation of progenitor cells to proceed during development of somites in the tail.
Title: Mesogenin causes embryonic mesoderm progenitors to differentiate during development of zebrafish tail somites.

mespo expression is intensified in the involuting mesoderm at 60% epiboly, and then restricted to the presomitic mesoderm (PSM) at 95% epiboly. At the 1-somite stage, mespo expression becomes reduced in the most rostral PSM.
Title: Characterization and expression of a presomitic mesoderm-specific mespo gene in zebrafish.

The following msgn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msgn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98361	NM_182882.2 Latest version!	Danio rerio mesogenin 1 (msgn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

msgn1 ( NM_182882.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_878302.1 Danio rerio mesogenin 1 (msgn1), mRNA.

Price & Availability↑

CloneID	ODa98361
Clone ID Related Accession (Same CDS sequence)	NM_182882.2
Accession Version	NM_182882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Danio rerio(zebrafish)
Product	mesogenin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH582872.1 and BX890562.8. On Feb 19, 2016 this sequence version replaced NM_182882.1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by partial transcript alignments, RNA-seq data and orthologous alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCGCAAA TCGACGTGGA TGTGTTCACA GCCAAGGTTT TGTCCCATTG GGACTGGAGC 
CGTGAGGACA GGTCATTTGG AGACAGCGCG TCCTCGCCGG AGTCCGAGTC CTTCGATTCT 
GCCTGCTCGT CGCCCGACGC GCGCTCCAGC CCCACAGCAG GGTGCGAGCA TGCGGAGCAG 
CAGAAGCCGA AAGTGAAGAT GAGTATGAGG AGGAGGATGA AGGCCAGCGA GCGGGAGAAG 
CTCCGCATGA GGAGTCTGGC GGAGGCGCTG CATCAGCTAC GGGATTACCT TCCGCCGGGG 
TACAGCAGGA GAGGACAGCC GCTCACCAAG ATCCAGACGC TCAAATACAC CATCCAGTAC 
ATCAAAGAGT TATCCGGCAT CCTCGAGCAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_878302.1
CDS	20..415
Translation	MAQIDVDVFTAKVLSHWDWSREDRSFGDSASSPESESFDSACSSPDARSSPTAGCEHAEQQKPKVKMSMRRRMKASEREKLRMRSLAEALHQLRDYLPPGYSRRGQPLTKIQTLKYTIQYIKELSGILEQQ

Target ORF information:

RefSeq Version	NM_182882.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mesogenin 1 (msgn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182882.2

ORF Insert Sequence:

ATGGCGCAAA TCGACGTGGA TGTGTTCACA GCCAAGGTTT TGTCCCATTG GGACTGGAGC 
CGTGAGGACA GGTCATTTGG AGACAGCGCG TCCTCGCCGG AGTCCGAGTC CTTCGATTCT 
GCCTGCTCGT CGCCCGACGC GCGCTCCAGC CCCACAGCAG GGTGCGAGCA TGCGGAGCAG 
CAGAAGCCGA AAGTGAAGAT GAGTATGAGG AGGAGGATGA AGGCCAGCGA GCGGGAGAAG 
CTCCGCATGA GGAGTCTGGC GGAGGCGCTG CATCAGCTAC GGGATTACCT TCCGCCGGGG 
TACAGCAGGA GAGGACAGCC GCTCACCAAG ATCCAGACGC TCAAATACAC CATCCAGTAC 
ATCAAAGAGT TATCCGGCAT CCTCGAGCAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation.
Developmental cell43(6)704-715.e5(2017 Dec)
Gross-Thebing T,Yigit S,Pfeiffer J,Reichman-Fried M,Bandemer J,Ruckert C,Rathmer C,Goudarzi M,Stehling M,Tarbashevich K,Seggewiss J,Raz E

tbx6l and tbx16 are redundantly required for posterior paraxial mesoderm formation during zebrafish embryogenesis.
Developmental dynamics : an official publication of the American Association of Anatomists246(10)759-769(2017 Oct)
Morrow ZT,Maxwell AM,Hoshijima K,Talbot JC,Grunwald DJ,Amacher SL

FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step epithelial to mesenchymal transition.
Development (Cambridge, England)144(8)1412-1424(2017 04)
Goto H,Kimmey SC,Row RH,Matus DQ,Martin BL