syt4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	syt4
Entrez Gene ID	335357
Full Name	synaptotagmin IV
Synonyms	wu:fj05d11,zgc:73240
General protein information	Preferred Names synaptotagmin IV Names synaptotagmin-4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199948.1	NP_956242.1	synaptotagmin-4

Pathways from BioSystems

Homologies

Homo sapiens (human)	SYT4	NP_065834.1
	SYT4	XP_001083607.1
Bos taurus (cattle)	SYT4	NP_001091577.1
Rattus norvegicus (Norway rat)	Syt4	NP_113881.1
Pan troglodytes (chimpanzee)	SYT4	XP_523915.3
Canis lupus familiaris (dog)	SYT4	XP_547596.2
Gallus gallus (chicken)	SYT4	XP_420894.1
Xenopus tropicalis (tropical clawed frog)	syt4	NP_001164985.1
Danio rerio (zebrafish)	syt4	NP_956242.1
Mus musculus (house mouse)	Syt4	NP_033334.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Maternal Rest/Nrsf Regulates Zebrafish Behavior through snap25a/b.
Moravec CE, Samuel J, Weng W, Wood IC, Sirotkin HI
The Journal of neuroscience : the official journal of the Society for Neuroscience36(36)9407-19(2016 09)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Rest represses maturation within migrating facial branchiomotor neurons.
Love CE, Prince VE
Developmental biology401(2)220-35(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following syt4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the syt4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31040	NM_199948.1 Latest version!	Danio rerio synaptotagmin IV (syt4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

syt4 ( NM_199948.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956242.1 Danio rerio synaptotagmin IV (syt4), mRNA.

Price & Availability↑

CloneID	ODa31040
Clone ID Related Accession (Same CDS sequence)	NM_199948.1
Accession Version	NM_199948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	synaptotagmin-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC059580.1. ##Evidence-Data-START## Transcript exon combination :: BC059580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCACCAG TGACGACAGA AGAAGCACAT TTTGCGGAGG TTCCTGTGAG CGTGGCAGTG 
GTCAGTGTGT TTGGTCTGGT CTTCAGTGTT TCCATCTTTG CATGGATTTG CTGTCAACGC 
AAAGCCAACA AGAGCAGCAA CAAGACCCCA CCCTACAAGT TCGTCCACAT GCTGAAGGGG 
GTTGACATCT ACCCTGAAAG CCTGAGTGGA AAGAAGAAGT TTGGAGGAGA AAAGACACCA 
GAAGCCCATG GAAAACAAAC CCTCAGTCCT ACTGGGGGAC GGCCAGACCT CCATCTAGAC 
CTGGAGAAAC GAGACCTAAA TGGAAACTTC ACCACAAAGC CACCGACACT CCAACTGAAG 
GTGCGTAGTT CTCCAGATAT TGACATCCCA GCTCTTCAGG GAGGATTTGC GAACCAGGAG 
GCTGGTACTC CAGAAAGCGT GGTATCCAGT CATACACCAA CACCAGCCGT GGAGAAATCC 
CAGGACAAGG AAGGTGGTCT GGGAACGCTC TTCTTTTCGG TTGAGTACAA CTTTGAGAAG 
AAGGCTTTCA TGGTTCACAT AAAGGAGGCA CATGGTCTGT CGCCCACAGA TGAACAGTCG 
CTGACCTCTG ACCCCTATAT TAAGCTGACC CTGCTGCCTG AGAAGAAGCA CAAGGTGAAG 
ACACGTGTTT TGAGGAAGAC TCTGGACCCG GCCTTTGACG AGACCTTCAG CTTCTATGGA 
ATCCCATTTG CACGAGTTTC CCAGCTGGCT CTGCACTTCA TGGTGCTGAG TTTCGACCGA 
TTTTCACGAG ATGAGGTCAT TGGAGAAACC CTTGTTCCCC TGGCTGATAT TGACCTGTCT 
GAGGGGCGCG TCCTCATGAG CCGAGACATT ATTAAGAAAA ATATCAGGAG GAGTGCTGGA 
CGAGGAGAGC TGCTTCTGTC CCTGTGTTAT CAGTCGACAA CCAGCACTCT GACTGTAGTG 
GTGCTGAAAG CCCGTCATTT GCCCAAAGCT GACTCCAGCG GACCCTCAGA CCCTTACGTG 
AAGGTGAACC TGTTCCAGGG GAAGAAGCGT GTATGCAAGA AGAAGACGCA TGTGAAAAAG 
TGTGCCCCCA ACCCAGTCTT CAACGAGCTC TTCGTCTTCG ACCTGCCCTC AGAAGACGGC 
CTGCGAGACA CCAGCGTGGA GCTTCTTCTG CTGGACTCCG ACAGGACGTC TCGTACGCCT 
GTCATTGGCC GGCTTGTTCT GGGAACCTCC TCTCCTGGCA CCGCAGGCGA GCACTGGCGC 
GAGATCTGTG ACCACCCGCG GCGACAGATC GCCAAGTGGC ATGCGCTTTC CGAAGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956242.1
CDS	192..1511
Translation	MAPVTTEEAHFAEVPVSVAVVSVFGLVFSVSIFAWICCQRKANKSSNKTPPYKFVHMLKGVDIYPESLSGKKKFGGEKTPEAHGKQTLSPTGGRPDLHLDLEKRDLNGNFTTKPPTLQLKVRSSPDIDIPALQGGFANQEAGTPESVVSSHTPTPAVEKSQDKEGGLGTLFFSVEYNFEKKAFMVHIKEAHGLSPTDEQSLTSDPYIKLTLLPEKKHKVKTRVLRKTLDPAFDETFSFYGIPFARVSQLALHFMVLSFDRFSRDEVIGETLVPLADIDLSEGRVLMSRDIIKKNIRRSAGRGELLLSLCYQSTTSTLTVVVLKARHLPKADSSGPSDPYVKVNLFQGKKRVCKKKTHVKKCAPNPVFNELFVFDLPSEDGLRDTSVELLLLDSDRTSRTPVIGRLVLGTSSPGTAGEHWREICDHPRRQIAKWHALSED

Target ORF information:

RefSeq Version	NM_199948.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synaptotagmin IV (syt4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199948.1

ORF Insert Sequence:

ATGGCACCAG TGACGACAGA AGAAGCACAT TTTGCGGAGG TTCCTGTGAG CGTGGCAGTG 
GTCAGTGTGT TTGGTCTGGT CTTCAGTGTT TCCATCTTTG CATGGATTTG CTGTCAACGC 
AAAGCCAACA AGAGCAGCAA CAAGACCCCA CCCTACAAGT TCGTCCACAT GCTGAAGGGG 
GTTGACATCT ACCCTGAAAG CCTGAGTGGA AAGAAGAAGT TTGGAGGAGA AAAGACACCA 
GAAGCCCATG GAAAACAAAC CCTCAGTCCT ACTGGGGGAC GGCCAGACCT CCATCTAGAC 
CTGGAGAAAC GAGACCTAAA TGGAAACTTC ACCACAAAGC CACCGACACT CCAACTGAAG 
GTGCGTAGTT CTCCAGATAT TGACATCCCA GCTCTTCAGG GAGGATTTGC GAACCAGGAG 
GCTGGTACTC CAGAAAGCGT GGTATCCAGT CATACACCAA CACCAGCCGT GGAGAAATCC 
CAGGACAAGG AAGGTGGTCT GGGAACGCTC TTCTTTTCGG TTGAGTACAA CTTTGAGAAG 
AAGGCTTTCA TGGTTCACAT AAAGGAGGCA CATGGTCTGT CGCCCACAGA TGAACAGTCG 
CTGACCTCTG ACCCCTATAT TAAGCTGACC CTGCTGCCTG AGAAGAAGCA CAAGGTGAAG 
ACACGTGTTT TGAGGAAGAC TCTGGACCCG GCCTTTGACG AGACCTTCAG CTTCTATGGA 
ATCCCATTTG CACGAGTTTC CCAGCTGGCT CTGCACTTCA TGGTGCTGAG TTTCGACCGA 
TTTTCACGAG ATGAGGTCAT TGGAGAAACC CTTGTTCCCC TGGCTGATAT TGACCTGTCT 
GAGGGGCGCG TCCTCATGAG CCGAGACATT ATTAAGAAAA ATATCAGGAG GAGTGCTGGA 
CGAGGAGAGC TGCTTCTGTC CCTGTGTTAT CAGTCGACAA CCAGCACTCT GACTGTAGTG 
GTGCTGAAAG CCCGTCATTT GCCCAAAGCT GACTCCAGCG GACCCTCAGA CCCTTACGTG 
AAGGTGAACC TGTTCCAGGG GAAGAAGCGT GTATGCAAGA AGAAGACGCA TGTGAAAAAG 
TGTGCCCCCA ACCCAGTCTT CAACGAGCTC TTCGTCTTCG ACCTGCCCTC AGAAGACGGC 
CTGCGAGACA CCAGCGTGGA GCTTCTTCTG CTGGACTCCG ACAGGACGTC TCGTACGCCT 
GTCATTGGCC GGCTTGTTCT GGGAACCTCC TCTCCTGGCA CCGCAGGCGA GCACTGGCGC 
GAGATCTGTG ACCACCCGCG GCGACAGATC GCCAAGTGGC ATGCGCTTTC CGAAGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Maternal Rest/Nrsf Regulates Zebrafish Behavior through snap25a/b.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(36)9407-19(2016 09)
Moravec CE,Samuel J,Weng W,Wood IC,Sirotkin HI

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Rest represses maturation within migrating facial branchiomotor neurons.
Developmental biology401(2)220-35(2015 May)
Love CE,Prince VE