rpa1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rpa1
Entrez Gene ID	327491
Full Name	replication protein A1
Synonyms	wu:fi14b08,zgc:55337,zgc:77092
General protein information	Preferred Names replication protein A1 Names replication protein A 70 kDa DNA-binding subunit RF-A protein 1 RP-A p70 replication factor A protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199811.2	NP_956105.2	replication protein A 70 kDa DNA-binding subunit

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RpA-70	NP_524274.1
Saccharomyces cerevisiae (baker's yeast)	RFA1	NP_009404.1
Kluyveromyces lactis	KLLA0A08844g	XP_451388.1
Eremothecium gossypii	AGOS_AFL008W	NP_985540.1
Arabidopsis thaliana (thale cress)	RPA70C	NP_199353.1
Mus musculus (house mouse)	Rpa1	NP_001157695.1
Rattus norvegicus (Norway rat)	Rpa1	NP_001041308.1
Danio rerio (zebrafish)	rpa1	NP_956105.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001421	XP_321709.4
Schizosaccharomyces pombe (fission yeast)	ssb1	NP_595092.1
Canis lupus familiaris (dog)	RPA1	XP_003639310.1
Oryza sativa (rice)	Os05g0111000	NP_001054445.1
Xenopus tropicalis (tropical clawed frog)	rpa1	NP_001015732.1
Homo sapiens (human)	RPA1	NP_002936.1
Pan troglodytes (chimpanzee)	RPA1	XP_511254.3
Bos taurus (cattle)	RPA1	NP_001068644.1
Gallus gallus (chicken)	RPA1	NP_001006221.1
Magnaporthe oryzae (rice blast fungus)	MGG_00685	XP_003718324.1
Neurospora crassa	NCU03606	XP_961333.1
Arabidopsis thaliana (thale cress)	AT4G19130	NP_567576.2

Gene Ontology
Bibliography

Related articles in PubMed

Xia H, Zhong C, Wu X, Chen J, Tao B, Xia X, Shi M, Zhu Z, Trudeau VL, Hu W
Genetics(2017 Dec)

The first characterization of multidrug and toxin extrusion (MATE/SLC47) proteins in zebrafish (Danio rerio).
Lon?ar J, Popovi? M, Krznar P, Zaja R, Smital T
Scientific reports628937(2016 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Distinct roles of Shh and Fgf signaling in regulating cell proliferation during zebrafish pectoral fin development.
Prykhozhij SV, Neumann CJ
BMC developmental biology891(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rpa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rpa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38838	NM_199811.2 Latest version!	Danio rerio replication protein A1 (rpa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rpa1 ( NM_199811.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956105.2 Danio rerio replication protein A1 (rpa1), mRNA.

Price & Availability↑

CloneID	ODa38838
Clone ID Related Accession (Same CDS sequence)	NM_199811.2
Accession Version	NM_199811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Danio rerio(zebrafish)
Product	replication protein A 70 kDa DNA-binding subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066711.1. On Feb 12, 2008 this sequence version replaced NM_199811.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066711.1, BC044372.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACTGTTA GACTGTCGGA AGGGGCAATT GAGTCCCTTT CAAAAGGAAC TGAAGTCAAC 
AACCCTATTC TTCAATGTGT GAACATCCGC AAGATTGATG GAGGTAATGG TGTGTCCCGT 
TTCCGTGTCA TGATGAGTGA TGGGCTGCAC ACTATGTCCT CGTTCATGCT GTCCACACAG 
CTCAATCCCA TGGCTGAGCA AAACCAGTTG GCCACTAACT GTGTTTGTGT GCTGAAAAGA 
AGCGTCACTA ATGTTCTCAA GGATGGACGA CGGGTGGTGG TTATACTGGA CATAGAAGTG 
CTCAAGTCTG CTGACCAAAT GCCGGGAAAG ATTGGAGACC CGACTCCTTA CGTTGAGGGT 
CAGTCAAAGG CACCGAGTAC AGCTCCAGCT CCAACTGCAC GTCCATTACA GCCACAGAAT 
GGAAGCGATG GTTCAACGTA CAGGCCTTCT GCTCAGAGCT TCGGGAAAAA ACCCATGGCT 
GCTCCCAACA CTCCTGGAGG CAGCTCCAAA GTTGTGCCTA TCGCCAGCCT CAATCCATAC 
CAGTCAAAAT GGACCATTAG AGCCAGAGTC ACTAACAAGA GTGCCATCCG CACATGGAGT 
AACTCCAGAG GGGATGGAAA ACTCTTCTCA ATGGAGCTTG TGGATGAAAG CGGGGAGATC 
AGAGCAACTG GATTCAATAA TGAGGTGGAC AAATTCTTCT CCCTCATCGA GCAGGGGAAA 
GTCTTCTACA TCTCCAAAGG CACCCTTAAG ATTGCTAACA AACAGTTCTC CTCACTCAAG 
AACGATTACG AGATGACTCT CAATGGCGAG ACAAGCATCA TCCCTTGTGA GGATAGTAAT 
GACGTCCCCA TGTTGCAGTG CGACTTTGTG TCCATTGCAG ACTTGGAGTC TCGGGAGAAA 
GACACCATCT TGGATGTGAT CGGAGTGTGT AAAAATGCAG AGGATGTGGC ACGCATCATG 
ACCAAGAACA GTCGTGAGGT ATCCAAGAGG AACATCCAAC TGATTGACAT GTCTGGCAGA 
GTCATCCAGC TGACCATGTG GGGCAGTGAT GCAGAGACGT TTGATGGCAG TGGTCAACCC 
ATCTTGGCCA TTAAGGGTGC AAGATTGTCT GACTTCGGTG GGCGTTCTCT CTCCACTCTC 
TATAGCAGCA CAGTCATGAT CAACCCTGAC ATTCCTGAGG CGTACAAACT GCGGGGCTGG 
TATGATAAGG AAGGACATGC ATTGGATGGA CAGTCCATGA CAGAGCTGAG AGGACCAGGT 
GGTGGTGGAA ACACAAACTG GAAGACCCTG GCGGAAGTCA AAAATGAGCA TCTTGGCCAT 
GGAGACAAAG CTGATTATTT CTCTTGCATC GCAACCATAG TCTACATCCG CAAAGAGAAC 
TGCTTGTATC AGGCCTGTCC CAGTAAAGAC TGCAATAAGA AGGTGGTGGA CCAGCAGAAT 
GGCATGTTCC GCTGTGAGAA ATGCGACAAA GAGTTCCCTG ACTTCAAATA TCGTCTCATG 
CTCTCGGCCA ACATTGCTGA TTTTGGAGAC AACCAGTGGG TGACTTGCTT TCAAGACACT 
GCCGAGACTC TTCTGGGCCA AAATTCTAGT TATCTTGGCC AACTAAAGGA CACAAATGAA 
GCTGCATTTG ATGAGGTCTT TCAGCATGCA AACTTCAATA CCTTTGTCTT CCGGAACCGA 
GTGAAGCTGG AGACCTACAA TGATGAATCG CGCATTAAAG TGACAGTGGT GGACGCCAAG 
CCTGTGGACC ACCGAGAATA CAGCAAACGG CTGATCATCA ACATAAGGAA GTTGGCTGCA 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956105.2
CDS	126..1931
Translation	MTVRLSEGAIESLSKGTEVNNPILQCVNIRKIDGGNGVSRFRVMMSDGLHTMSSFMLSTQLNPMAEQNQLATNCVCVLKRSVTNVLKDGRRVVVILDIEVLKSADQMPGKIGDPTPYVEGQSKAPSTAPAPTARPLQPQNGSDGSTYRPSAQSFGKKPMAAPNTPGGSSKVVPIASLNPYQSKWTIRARVTNKSAIRTWSNSRGDGKLFSMELVDESGEIRATGFNNEVDKFFSLIEQGKVFYISKGTLKIANKQFSSLKNDYEMTLNGETSIIPCEDSNDVPMLQCDFVSIADLESREKDTILDVIGVCKNAEDVARIMTKNSREVSKRNIQLIDMSGRVIQLTMWGSDAETFDGSGQPILAIKGARLSDFGGRSLSTLYSSTVMINPDIPEAYKLRGWYDKEGHALDGQSMTELRGPGGGGNTNWKTLAEVKNEHLGHGDKADYFSCIATIVYIRKENCLYQACPSKDCNKKVVDQQNGMFRCEKCDKEFPDFKYRLMLSANIADFGDNQWVTCFQDTAETLLGQNSSYLGQLKDTNEAAFDEVFQHANFNTFVFRNRVKLETYNDESRIKVTVVDAKPVDHREYSKRLIINIRKLAAQ

Target ORF information:

RefSeq Version	NM_199811.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio replication protein A1 (rpa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199811.2

ORF Insert Sequence:

ATGACTGTTA GACTGTCGGA AGGGGCAATT GAGTCCCTTT CAAAAGGAAC TGAAGTCAAC 
AACCCTATTC TTCAATGTGT GAACATCCGC AAGATTGATG GAGGTAATGG TGTGTCCCGT 
TTCCGTGTCA TGATGAGTGA TGGGCTGCAC ACTATGTCCT CGTTCATGCT GTCCACACAG 
CTCAATCCCA TGGCTGAGCA AAACCAGTTG GCCACTAACT GTGTTTGTGT GCTGAAAAGA 
AGCGTCACTA ATGTTCTCAA GGATGGACGA CGGGTGGTGG TTATACTGGA CATAGAAGTG 
CTCAAGTCTG CTGACCAAAT GCCGGGAAAG ATTGGAGACC CGACTCCTTA CGTTGAGGGT 
CAGTCAAAGG CACCGAGTAC AGCTCCAGCT CCAACTGCAC GTCCATTACA GCCACAGAAT 
GGAAGCGATG GTTCAACGTA CAGGCCTTCT GCTCAGAGCT TCGGGAAAAA ACCCATGGCT 
GCTCCCAACA CTCCTGGAGG CAGCTCCAAA GTTGTGCCTA TCGCCAGCCT CAATCCATAC 
CAGTCAAAAT GGACCATTAG AGCCAGAGTC ACTAACAAGA GTGCCATCCG CACATGGAGT 
AACTCCAGAG GGGATGGAAA ACTCTTCTCA ATGGAGCTTG TGGATGAAAG CGGGGAGATC 
AGAGCAACTG GATTCAATAA TGAGGTGGAC AAATTCTTCT CCCTCATCGA GCAGGGGAAA 
GTCTTCTACA TCTCCAAAGG CACCCTTAAG ATTGCTAACA AACAGTTCTC CTCACTCAAG 
AACGATTACG AGATGACTCT CAATGGCGAG ACAAGCATCA TCCCTTGTGA GGATAGTAAT 
GACGTCCCCA TGTTGCAGTG CGACTTTGTG TCCATTGCAG ACTTGGAGTC TCGGGAGAAA 
GACACCATCT TGGATGTGAT CGGAGTGTGT AAAAATGCAG AGGATGTGGC ACGCATCATG 
ACCAAGAACA GTCGTGAGGT ATCCAAGAGG AACATCCAAC TGATTGACAT GTCTGGCAGA 
GTCATCCAGC TGACCATGTG GGGCAGTGAT GCAGAGACGT TTGATGGCAG TGGTCAACCC 
ATCTTGGCCA TTAAGGGTGC AAGATTGTCT GACTTCGGTG GGCGTTCTCT CTCCACTCTC 
TATAGCAGCA CAGTCATGAT CAACCCTGAC ATTCCTGAGG CGTACAAACT GCGGGGCTGG 
TATGATAAGG AAGGACATGC ATTGGATGGA CAGTCCATGA CAGAGCTGAG AGGACCAGGT 
GGTGGTGGAA ACACAAACTG GAAGACCCTG GCGGAAGTCA AAAATGAGCA TCTTGGCCAT 
GGAGACAAAG CTGATTATTT CTCTTGCATC GCAACCATAG TCTACATCCG CAAAGAGAAC 
TGCTTGTATC AGGCCTGTCC CAGTAAAGAC TGCAATAAGA AGGTGGTGGA CCAGCAGAAT 
GGCATGTTCC GCTGTGAGAA ATGCGACAAA GAGTTCCCTG ACTTCAAATA TCGTCTCATG 
CTCTCGGCCA ACATTGCTGA TTTTGGAGAC AACCAGTGGG TGACTTGCTT TCAAGACACT 
GCCGAGACTC TTCTGGGCCA AAATTCTAGT TATCTTGGCC AACTAAAGGA CACAAATGAA 
GCTGCATTTG ATGAGGTCTT TCAGCATGCA AACTTCAATA CCTTTGTCTT CCGGAACCGA 
GTGAAGCTGG AGACCTACAA TGATGAATCG CGCATTAAAG TGACAGTGGT GGACGCCAAG 
CCTGTGGACC ACCGAGAATA CAGCAAACGG CTGATCATCA ACATAAGGAA GTTGGCTGCA 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetics(2017 Dec)
Xia H,Zhong C,Wu X,Chen J,Tao B,Xia X,Shi M,Zhu Z,Trudeau VL,Hu W

The first characterization of multidrug and toxin extrusion (MATE/SLC47) proteins in zebrafish (Danio rerio).
Scientific reports628937(2016 Jun)
Lon?ar J,Popovi? M,Krznar P,Zaja R,Smital T

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Distinct roles of Shh and Fgf signaling in regulating cell proliferation during zebrafish pectoral fin development.
BMC developmental biology891(2008 Sep)
Prykhozhij SV,Neumann CJ