evx2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	evx2
Entrez Gene ID	30479
Full Name	even-skipped homeobox 2
Synonyms	Evx-2
General protein information	Preferred Names even-skipped homeobox 2 Names homeobox even-skipped homolog protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131232.2	NP_571307.2	homeobox even-skipped homolog protein 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EVX2	XP_852353.1
Rattus norvegicus (Norway rat)	Evx2	XP_221512.5
Pan troglodytes (chimpanzee)	EVX2	XP_525966.2
Homo sapiens (human)	EVX2	NP_001073927.1
	EVX2	XP_001094253.1
Mus musculus (house mouse)	Evx2	NP_031993.1
Gallus gallus (chicken)	EVX2	XP_001234658.3
Xenopus tropicalis (tropical clawed frog)	evx2	XP_002935724.2
Danio rerio (zebrafish)	evx2	NP_571307.2

Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish transgenic constructs label specific neurons in Xenopus laevis spinal cord and identify frog V0v spinal neurons.
Ju?rez-Morales JL, Martinez-De Luna RI, Zuber ME, Roberts A, Lewis KE
Developmental neurobiology77(8)1007-1020(2017 Sep)

Lmx1b is required for the glutamatergic fates of a subset of spinal cord neurons.
Hilinski WC, Bostrom JR, England SJ, Ju?rez-Morales JL, de Jager S, Armant O, Legradi J, Str?hle U, Link BA, Lewis KE
Neural development11(1)16(2016 08)

Evx1 and Evx2 specify excitatory neurotransmitter fates and suppress inhibitory fates through a Pax2-independent mechanism.
Ju?rez-Morales JL, Schulte CJ, Pezoa SA, Vallejo GK, Hilinski WC, England SJ, de Jager S, Lewis KE
Neural development115(2016 Feb)

A 3D Searchable Database of Transgenic Zebrafish Gal4 and Cre Lines for Functional Neuroanatomy Studies.
Marquart GD, Tabor KM, Brown M, Strykowski JL, Varshney GK, LaFave MC, Mueller T, Burgess SM, Higashijima S, Burgess HA
Frontiers in neural circuits978(2015)

Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering.
Kimura Y, Hisano Y, Kawahara A, Higashijima S
Scientific reports46545(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrates that Evx1 and Evx2 are expressed by spinal cord V0v cells and show that these cells develop into excitatory (glutamatergic) Commissural Ascending (CoSA) interneurons. Evx1 and Evx2 are required, partially redundantly, for spinal cord V0v cells to become excitatory (glutamatergic) interneurons.
Title: Evx1 and Evx2 specify excitatory neurotransmitter fates and suppress inhibitory fates through a Pax2-independent mechanism.

The following evx2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the evx2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31202	NM_131232.2 Latest version!	Danio rerio even-skipped homeobox 2 (evx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

evx2 ( NM_131232.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571307.2 Danio rerio even-skipped homeobox 2 (evx2), mRNA.

Price & Availability↑

CloneID	ODa31202
Clone ID Related Accession (Same CDS sequence)	NM_131232.2
Accession Version	NM_131232.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-16
Organism	Danio rerio(zebrafish)
Product	homeobox even-skipped homolog protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057443.1. On Jan 25, 2004 this sequence version replaced NM_131232.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGATGGAGA GGATAAGAAA AGAAATGATT CTGATGGAAA GGGGACTACA CAGTCCAGTG 
GCAGGGAAGA GGCTCTCCAA CCTCACCGAC TCGGCCGGGA ACGCGGTGCT CGAGGCCCTG 
GAAAATTCCC AGCACGCAGG TCGCCTCAGC CCGAGAATAA CTTCCGCCTC GCTGCATGGG 
GATATTCCCA CCAAAGGCAA GTTCGAGATC GACAGCTTAT TTGGGAACCA CCACAACGAC 
AACACTTCCT CAACAGAAGT GTCGTCCTCC GAAAGCCGGA AGAAAATAAA CCTGTATCCT 
GAAGTTTCTC CTGACTCCGA CATGAACAGC GATGTGGAAG TGGGCTGCCC GTCTCATCGT 
TCCCCGGGCA GCATCAGCCA ACACAAGGAG AACAACAATA AAGGTTTCTC GGACAGCAAT 
TCAGGAGGCT CCAATACAAG TTCATCCTCT CTATCGAATC TTAACGGTAA TTCCATGGGA 
AACAGCAGCT CGAGCTCGGA CCAGGTGAGG AGGTACCGGA CTGCTTTTAC CCGAGAGCAG 
ATTGGAAGAC TGGAGAAAGA ATTTTACAGG GAAAACTACG TCTCGAGACC CAGGAGATGT 
GAATTAGCCG CAGCATTAAA CCTGCCCGAA ACAACAATAA AGGTGTGGTT TCAGAATCGA 
CGAATGAAGG ACAAGCGGCA GCGGCTTGCC ATGTCCTGGC CACATCCCGC CGATCCCAGC 
TTCTACACCT ATATGATGAC GCACGCCGCC GCCACCGGAA GTCTGCCTTA CCCTTTTCAT 
TCCCACATGC CACTACACTA TTACCCCCAT GTCGGTGTCA CGGCAGCAGC TGCTGCGGCA 
GCCGCCTCAG GAGCCGCATC CTCACCTTTT GCTACCTCTA TACGTCCCCT TGATACTTTC 
CGTGCGCTTT CTCATCCATA TTCGCGCCCA GAGCTGCTGT GTAGCTTTCG ACATCCAGGA 
CTGTACCAGT CACCCGCTGG TTTAAACAGC TCGGCGGCTG CGTCGGCGGC AGCGGCGGCG 
GCGGCAGCGG CTGCGGCGGT CAGCGCGCCC TCGGCTACCG GGCCGTGCTC GTGTCTCAGC 
TGCCACAGCA GTCAGGCCGC CAGCGCACTG GGCTCCAGGA GCTCCAGCTC TGATTTTACC 
TGCACGGCGA CCGGGCAAAG GTCTGAGAGC GGATTTTTGC CATACTCGGC GGCAGTCCTC 
AGCAAAACCG CGGTGCCCTC ACCGGACCAG AGAGAAGAAA GCGCGCTTAA TAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571307.2
CDS	363..1619
Translation	MMERIRKEMILMERGLHSPVAGKRLSNLTDSAGNAVLEALENSQHAGRLSPRITSASLHGDIPTKGKFEIDSLFGNHHNDNTSSTEVSSSESRKKINLYPEVSPDSDMNSDVEVGCPSHRSPGSISQHKENNNKGFSDSNSGGSNTSSSSLSNLNGNSMGNSSSSSDQVRRYRTAFTREQIGRLEKEFYRENYVSRPRRCELAAALNLPETTIKVWFQNRRMKDKRQRLAMSWPHPADPSFYTYMMTHAAATGSLPYPFHSHMPLHYYPHVGVTAAAAAAAASGAASSPFATSIRPLDTFRALSHPYSRPELLCSFRHPGLYQSPAGLNSSAAASAAAAAAAAAAAVSAPSATGPCSCLSCHSSQAASALGSRSSSSDFTCTATGQRSESGFLPYSAAVLSKTAVPSPDQREESALNR

Target ORF information:

RefSeq Version	NM_131232.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio even-skipped homeobox 2 (evx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131232.2

ORF Insert Sequence:

ATGATGGAGA GGATAAGAAA AGAAATGATT CTGATGGAAA GGGGACTACA CAGTCCAGTG 
GCAGGGAAGA GGCTCTCCAA CCTCACCGAC TCGGCCGGGA ACGCGGTGCT CGAGGCCCTG 
GAAAATTCCC AGCACGCAGG TCGCCTCAGC CCGAGAATAA CTTCCGCCTC GCTGCATGGG 
GATATTCCCA CCAAAGGCAA GTTCGAGATC GACAGCTTAT TTGGGAACCA CCACAACGAC 
AACACTTCCT CAACAGAAGT GTCGTCCTCC GAAAGCCGGA AGAAAATAAA CCTGTATCCT 
GAAGTTTCTC CTGACTCCGA CATGAACAGC GATGTGGAAG TGGGCTGCCC GTCTCATCGT 
TCCCCGGGCA GCATCAGCCA ACACAAGGAG AACAACAATA AAGGTTTCTC GGACAGCAAT 
TCAGGAGGCT CCAATACAAG TTCATCCTCT CTATCGAATC TTAACGGTAA TTCCATGGGA 
AACAGCAGCT CGAGCTCGGA CCAGGTGAGG AGGTACCGGA CTGCTTTTAC CCGAGAGCAG 
ATTGGAAGAC TGGAGAAAGA ATTTTACAGG GAAAACTACG TCTCGAGACC CAGGAGATGT 
GAATTAGCCG CAGCATTAAA CCTGCCCGAA ACAACAATAA AGGTGTGGTT TCAGAATCGA 
CGAATGAAGG ACAAGCGGCA GCGGCTTGCC ATGTCCTGGC CACATCCCGC CGATCCCAGC 
TTCTACACCT ATATGATGAC GCACGCCGCC GCCACCGGAA GTCTGCCTTA CCCTTTTCAT 
TCCCACATGC CACTACACTA TTACCCCCAT GTCGGTGTCA CGGCAGCAGC TGCTGCGGCA 
GCCGCCTCAG GAGCCGCATC CTCACCTTTT GCTACCTCTA TACGTCCCCT TGATACTTTC 
CGTGCGCTTT CTCATCCATA TTCGCGCCCA GAGCTGCTGT GTAGCTTTCG ACATCCAGGA 
CTGTACCAGT CACCCGCTGG TTTAAACAGC TCGGCGGCTG CGTCGGCGGC AGCGGCGGCG 
GCGGCAGCGG CTGCGGCGGT CAGCGCGCCC TCGGCTACCG GGCCGTGCTC GTGTCTCAGC 
TGCCACAGCA GTCAGGCCGC CAGCGCACTG GGCTCCAGGA GCTCCAGCTC TGATTTTACC 
TGCACGGCGA CCGGGCAAAG GTCTGAGAGC GGATTTTTGC CATACTCGGC GGCAGTCCTC 
AGCAAAACCG CGGTGCCCTC ACCGGACCAG AGAGAAGAAA GCGCGCTTAA TAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish transgenic constructs label specific neurons in Xenopus laevis spinal cord and identify frog V0v spinal neurons.
Developmental neurobiology77(8)1007-1020(2017 Sep)
Ju?rez-Morales JL,Martinez-De Luna RI,Zuber ME,Roberts A,Lewis KE

Lmx1b is required for the glutamatergic fates of a subset of spinal cord neurons.
Neural development11(1)16(2016 08)
Hilinski WC,Bostrom JR,England SJ,Ju?rez-Morales JL,de Jager S,Armant O,Legradi J,Str?hle U,Link BA,Lewis KE

Evx1 and Evx2 specify excitatory neurotransmitter fates and suppress inhibitory fates through a Pax2-independent mechanism.
Neural development115(2016 Feb)
Ju?rez-Morales JL,Schulte CJ,Pezoa SA,Vallejo GK,Hilinski WC,England SJ,de Jager S,Lewis KE

A 3D Searchable Database of Transgenic Zebrafish Gal4 and Cre Lines for Functional Neuroanatomy Studies.
Frontiers in neural circuits978(2015)
Marquart GD,Tabor KM,Brown M,Strykowski JL,Varshney GK,LaFave MC,Mueller T,Burgess SM,Higashijima S,Burgess HA

Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering.
Scientific reports46545(2014 Oct)
Kimura Y,Hisano Y,Kawahara A,Higashijima S