SHHA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SHHA
Entrez Gene ID	30269
Full Name	sonic hedgehog a
Synonyms	ShhNC,fc83d08,shh,syu,vhh-1,vhh1,wu:fc83d08
General protein information	Preferred Names sonic hedgehog a Names sonic hedgehog protein A shh unprocessed N-terminal signaling and C-terminal autoprocessing domains sonic-you
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131063.3	NP_571138.1	sonic hedgehog protein A precursor
XM_009303382.2	XP_009301657.1	sonic hedgehog protein A isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	shh	XP_002932544.2
Homo sapiens (human)	SHH	NP_000184.1
Mus musculus (house mouse)	Shh	NP_033196.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001412	XP_321721.5
Bos taurus (cattle)	SHH	XP_002687031.2
Rattus norvegicus (Norway rat)	Shh	NP_058917.1
Gallus gallus (chicken)	SHH	NP_990152.1
Danio rerio (zebrafish)	shha	NP_571138.1
Danio rerio (zebrafish)	shhb	NP_571274.2
	SHH	XP_001106515.2
Canis lupus familiaris (dog)	SHH	XP_850450.1
Pan troglodytes (chimpanzee)	SHH	XP_001147185.2
Drosophila melanogaster (fruit fly)	hh	NP_001034065.1

Gene Ontology

The following SHHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02261	NM_131063.3 Latest version!	Danio rerio sonic hedgehog signaling molecule a (shha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa39205	XM_009303382.2 Latest version!	Danio rerio sonic hedgehog a (shha), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHHA ( NM_131063.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571138.1 Danio rerio sonic hedgehog signaling molecule a (shha), mRNA.

Price & Availability↑

CloneID	ODa02261
Clone ID Related Accession (Same CDS sequence)	NM_131063.3
Accession Version	NM_131063.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	sonic hedgehog protein A precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L27585.1, CU041319.16 and CT668143.2. On Jun 11, 2014 this sequence version replaced NM_131063.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L27585.1, BC162395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTTT TGACGAGAGT GCTGCTGGTG TCTCTTCTCA CTCTGTCCTT GGTGGTGTCC 
GGACTGGCCT GCGGTCCTGG CAGAGGCTAC GGCAGAAGAA GACATCCGAA GAAGCTGACA 
CCTCTCGCCT ACAAGCAGTT CATACCTAAT GTCGCGGAGA AGACCTTAGG GGCCAGCGGC 
AGATACGAGG GCAAGATAAC GCGCAATTCG GAGAGATTTA AAGAACTTAC TCCAAATTAT 
AATCCCGACA TTATCTTTAA GGATGAGGAG AACACGGGAG CGGACAGGCT CATGACACAG 
AGATGCAAAG ACAAGCTGAA CTCGCTGGCC ATCTCTGTAA TGAACCACTG GCCAGGGGTT 
AAGCTGCGTG TGACAGAGGG CTGGGATGAG GACGGTCACC ATTTTGAAGA ATCACTCCAC 
TACGAGGGAA GAGCTGTTGA TATTACCACC TCTGACCGAG ACAAGAGCAA ATACGGGACA 
CTGTCTCGCC TAGCTGTGGA GGCTGGATTT GACTGGGTCT ATTACGAGTC CAAAGCCCAC 
ATTCATTGCT CTGTCAAAGC AGAAAATTCG GTTGCTGCGA AATCTGGGGG CTGTTTCCCA 
GGTTCGGCTC TGGTCTCGCT CCAGGACGGA GGACAGAAGG CCGTGAAGGA CCTGAACCCC 
GGAGACAAGG TGCTGGCGGC AGACAGCGCG GGAAACCTGG TGTTCAGCGA CTTCATCATG 
TTCACAGACC GAGACTCCAC GACGCGACGT GTGTTTTACG TCATAGAAAC GCAAGAACCC 
GTTGAAAAGA TCACCCTCAC CGCCGCTCAC CTCCTTTTTG TCCTCGACAA CTCAACGGAA 
GATCTCCACA CCATGACCGC CGCGTATGCC AGCAGTGTCA GAGCCGGACA AAAGGTGATG 
GTTGTTGATG ATAGCGGCCA GCTTAAATCT GTCATCGTGC AGCGGATATA CACGGAGGAG 
CAGCGGGGCT CGTTCGCACC AGTGACTGCA CATGGGACCA TTGTGGTCGA CAGAATACTG 
GCGTCCTGTT ACGCCGTAAT AGAGGACCAG GGGCTTGCGC ATTTGGCCTT CGCGCCCGCC 
AGGCTGTATT ATTACGTGTC ATCATTCCTG TTCCCCCAAA ACTCCAGCAG TCGGTCCAAT 
GCGACTTTAC AACAGGAGGG GGTCCACTGG TACTCCAGGC TCCTGTATCA AATGGGAACG 
TGGCTTTTGG ACAGCAACAT GCTTCATCCT TTGGGGATGT CAGTAAACTC AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571138.1
CDS	320..1576
Translation	MRLLTRVLLVSLLTLSLVVSGLACGPGRGYGRRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNSLAISVMNHWPGVKLRVTEGWDEDGHHFEESLHYEGRAVDITTSDRDKSKYGTLSRLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSALVSLQDGGQKAVKDLNPGDKVLAADSAGNLVFSDFIMFTDRDSTTRRVFYVIETQEPVEKITLTAAHLLFVLDNSTEDLHTMTAAYASSVRAGQKVMVVDDSGQLKSVIVQRIYTEEQRGSFAPVTAHGTIVVDRILASCYAVIEDQGLAHLAFAPARLYYYVSSFLFPQNSSSRSNATLQQEGVHWYSRLLYQMGTWLLDSNMLHPLGMSVNSS

Target ORF information:

RefSeq Version	NM_131063.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sonic hedgehog signaling molecule a (shha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131063.3

ORF Insert Sequence:

ATGCGGCTTT TGACGAGAGT GCTGCTGGTG TCTCTTCTCA CTCTGTCCTT GGTGGTGTCC 
GGACTGGCCT GCGGTCCTGG CAGAGGCTAC GGCAGAAGAA GACATCCGAA GAAGCTGACA 
CCTCTCGCCT ACAAGCAGTT CATACCTAAT GTCGCGGAGA AGACCTTAGG GGCCAGCGGC 
AGATACGAGG GCAAGATAAC GCGCAATTCG GAGAGATTTA AAGAACTTAC TCCAAATTAT 
AATCCCGACA TTATCTTTAA GGATGAGGAG AACACGGGAG CGGACAGGCT CATGACACAG 
AGATGCAAAG ACAAGCTGAA CTCGCTGGCC ATCTCTGTAA TGAACCACTG GCCAGGGGTT 
AAGCTGCGTG TGACAGAGGG CTGGGATGAG GACGGTCACC ATTTTGAAGA ATCACTCCAC 
TACGAGGGAA GAGCTGTTGA TATTACCACC TCTGACCGAG ACAAGAGCAA ATACGGGACA 
CTGTCTCGCC TAGCTGTGGA GGCTGGATTT GACTGGGTCT ATTACGAGTC CAAAGCCCAC 
ATTCATTGCT CTGTCAAAGC AGAAAATTCG GTTGCTGCGA AATCTGGGGG CTGTTTCCCA 
GGTTCGGCTC TGGTCTCGCT CCAGGACGGA GGACAGAAGG CCGTGAAGGA CCTGAACCCC 
GGAGACAAGG TGCTGGCGGC AGACAGCGCG GGAAACCTGG TGTTCAGCGA CTTCATCATG 
TTCACAGACC GAGACTCCAC GACGCGACGT GTGTTTTACG TCATAGAAAC GCAAGAACCC 
GTTGAAAAGA TCACCCTCAC CGCCGCTCAC CTCCTTTTTG TCCTCGACAA CTCAACGGAA 
GATCTCCACA CCATGACCGC CGCGTATGCC AGCAGTGTCA GAGCCGGACA AAAGGTGATG 
GTTGTTGATG ATAGCGGCCA GCTTAAATCT GTCATCGTGC AGCGGATATA CACGGAGGAG 
CAGCGGGGCT CGTTCGCACC AGTGACTGCA CATGGGACCA TTGTGGTCGA CAGAATACTG 
GCGTCCTGTT ACGCCGTAAT AGAGGACCAG GGGCTTGCGC ATTTGGCCTT CGCGCCCGCC 
AGGCTGTATT ATTACGTGTC ATCATTCCTG TTCCCCCAAA ACTCCAGCAG TCGGTCCAAT 
GCGACTTTAC AACAGGAGGG GGTCCACTGG TACTCCAGGC TCCTGTATCA AATGGGAACG 
TGGCTTTTGG ACAGCAACAT GCTTCATCCT TTGGGGATGT CAGTAAACTC AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHHA ( XM_009303382.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301657.1 Danio rerio sonic hedgehog a (shha), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39205
Clone ID Related Accession (Same CDS sequence)	XM_009303382.2
Accession Version	XM_009303382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sonic hedgehog protein A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACT GGCCAGGGGT TAAGCTGCGT GTGACAGAGG GCTGGGATGA GGACGGTCAC 
CATTTTGAAG AATCACTCCA CTACGAGGGA AGAGCTGTTG ATATTACCAC CTCTGACCGA 
GACAAGAGCA AATACGGGAC ACTGTCTCGC CTAGCTGTGG AGGCTGGATT TGACTGGGTC 
TATTACGAGT CCAAAGCCCA CATTCATTGC TCTGTCAAAG CAGAAAATTC GGTTGCTGCG 
AAATCTGGGG GCTGTTTCCC AGGTTCGGCT CTGGTCTCGC TCCAGGACGG AGGACAGAAG 
GCCGTGAAGG ACCTGAACCC CGGAGACAAG GTGCTGGCGG CAGACAGCGC GGGAAACCTG 
GTGTTCAGCG ACTTCATCAT GTTCACAGAC CGAGACTCCA CGACGCGACG TGTGTTTTAC 
GTCATAGAAA CGCAAGAACC CGTTGAAAAG ATCACCCTCA CCGCCGCTCA CCTCCTTTTT 
GTCCTCGACA ACTCAACGGA AGATCTCCAC ACCATGACCG CCGCGTATGC CAGCAGTGTC 
AGAGCCGGAC AAAAGGTGAT GGTTGTTGAT GATAGCGGCC AGCTTAAATC TGTCATCGTG 
CAGCGGATAT ACACGGAGGA GCAGCGGGGC TCGTTCGCAC CAGTGACTGC ACATGGGACC 
ATTGTGGTCG ACAGAATACT GGCGTCCTGT TACGCCGTAA TAGAGGACCA GGGGCTTGCG 
CATTTGGCCT TCGCGCCCGC CAGGCTGTAT TATTACGTGT CATCATTCCT GTTCCCCCAA 
AACTCCAGCA GTCGGTCCAA TGCGACTTTA CAACAGGAGG GGGTCCACTG GTACTCCAGG 
CTCCTGTATC AAATGGGAAC GTGGCTTTTG GACAGCAACA TGCTTCATCC TTTGGGGATG 
TCAGTAAACT CAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301657.1
CDS	183..1100
Translation	MNHWPGVKLRVTEGWDEDGHHFEESLHYEGRAVDITTSDRDKSKYGTLSRLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSALVSLQDGGQKAVKDLNPGDKVLAADSAGNLVFSDFIMFTDRDSTTRRVFYVIETQEPVEKITLTAAHLLFVLDNSTEDLHTMTAAYASSVRAGQKVMVVDDSGQLKSVIVQRIYTEEQRGSFAPVTAHGTIVVDRILASCYAVIEDQGLAHLAFAPARLYYYVSSFLFPQNSSSRSNATLQQEGVHWYSRLLYQMGTWLLDSNMLHPLGMSVNSS

Target ORF information:

RefSeq Version	XM_009303382.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sonic hedgehog a (shha), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303382.2

ORF Insert Sequence:

ATGAACCACT GGCCAGGGGT TAAGCTGCGT GTGACAGAGG GCTGGGATGA GGACGGTCAC 
CATTTTGAAG AATCACTCCA CTACGAGGGA AGAGCTGTTG ATATTACCAC CTCTGACCGA 
GACAAGAGCA AATACGGGAC ACTGTCTCGC CTAGCTGTGG AGGCTGGATT TGACTGGGTC 
TATTACGAGT CCAAAGCCCA CATTCATTGC TCTGTCAAAG CAGAAAATTC GGTTGCTGCG 
AAATCTGGGG GCTGTTTCCC AGGTTCGGCT CTGGTCTCGC TCCAGGACGG AGGACAGAAG 
GCCGTGAAGG ACCTGAACCC CGGAGACAAG GTGCTGGCGG CAGACAGCGC GGGAAACCTG 
GTGTTCAGCG ACTTCATCAT GTTCACAGAC CGAGACTCCA CGACGCGACG TGTGTTTTAC 
GTCATAGAAA CGCAAGAACC CGTTGAAAAG ATCACCCTCA CCGCCGCTCA CCTCCTTTTT 
GTCCTCGACA ACTCAACGGA AGATCTCCAC ACCATGACCG CCGCGTATGC CAGCAGTGTC 
AGAGCCGGAC AAAAGGTGAT GGTTGTTGAT GATAGCGGCC AGCTTAAATC TGTCATCGTG 
CAGCGGATAT ACACGGAGGA GCAGCGGGGC TCGTTCGCAC CAGTGACTGC ACATGGGACC 
ATTGTGGTCG ACAGAATACT GGCGTCCTGT TACGCCGTAA TAGAGGACCA GGGGCTTGCG 
CATTTGGCCT TCGCGCCCGC CAGGCTGTAT TATTACGTGT CATCATTCCT GTTCCCCCAA 
AACTCCAGCA GTCGGTCCAA TGCGACTTTA CAACAGGAGG GGGTCCACTG GTACTCCAGG 
CTCCTGTATC AAATGGGAAC GTGGCTTTTG GACAGCAACA TGCTTCATCC TTTGGGGATG 
TCAGTAAACT CAAGCTGA